Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005460

Physical Location & Seq

Genomic Coordinates Reverse (-)

31600113 .. 31608601

Gene Structure 8489 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1237800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3030 bp

ATGAACCAGAACCACAATTACAAGAGCAGCAGCAATGCCTCCAACAAAGAGATGGTAATCATGGACGCCCGACCCGGAAAGCTGAACAGCCGATCCAATTCCATATCCGACCCCAACACCAGCCAGAGCCTCGCCTCCATCCTCAACAACCCCAATGCCTCCGACGCCTCCTCCTGGGTCGGCTGGTGGTCCTCCTCCGCCTCCGTTGCCCCGCCCGAATTCGCACCTCTCGTGCCGAAATCTGCGTCCGACGCCGTGACCCGATCCGATTTCCAGCCCTACCTCGCCTCCGTCTCCGATCACTACAACCGCTTCGAGGACATCATCAACCACGTGAAGAAGGAGAAATCCGACGTCGATTCCATCGGTGGCCAAGGCGAAGCCCTCGTCGCGTGCCTCCGGGAGGTTCCGGCGCTCTATTTCAAGGAGGACTTCGCCCTTGAGGATGGCGCCACGTTCCGGTCGGCTTGCCCGTTCACGAACGTCTCAGAGAACCTGGGGCTGCAGGAGAAGCTGTCGCATTACTTGGACGTGGTGGAGCTTCACTTGGTGAAGGAGATCTCGCTGCGTTCCAACTCCTTCTTTGAGGCGCAGGGGCAGCTGGAGGACTTGAATGTCAAGATCGTCGAGGGGTGCAGTCGGATTCGAGAGCTCAAGGAGACGATTCTGCTCTTGGATGTCGATTTGGTCGAGTGCGCAACGCAGATTCATGATTTGAATGAAACCAGGAGCAATTTGTTGGCTCTGCAGCAGAAATTGAGGCTCATTTTATATGTTAATCAAGCTCTTTCGGCACTTAAACTGCTTGTTGCCTCTGCAGATTGTGCTGGAGCCTTGGACGTCACAGATGATTTACAGCATTTGCTGGATGGAGACGAGCTTACTGGTCTACATTGCTTCCATCACCTTAGGGATCGTGTAGCGGCTTCAATTGAATCCATAAACAGCATTCTTTCTGCAGAGTTTATGCGTGCTTCAATACACGATGCTGGAGATACAGATGTTATAATTATATCCAGAGCGAAAGCAAGGGCATCTAGTCTGATGAATGGAGAAGATGGTGAAATTAAGTTGGATGATGAAGAAACCTCCAATTTTCAAGATCGTCTTCTTCCTGTCATTATTGGGTTGCTTAGAACAGCCAAGCTCCCTTCTGTGTTGAGGTTATATCGTGACCAACTTACAGCTGACATGAAGTCTGCTATAAAAAATGCTGTTGCAGAGCTGCTTCCCATTCTTGTTTCGCGACCACTGGAGTCAGATTTTACACCGGGGGAGCGAATAGTTGATGCAGATGGTTTTGGCGCATCACTTGCGAGCAAGTTGAGGAGCCTGTCATCTGAAAGCTTTGTTCAACTTTTAAGTGCTATTTTTCTGATTGTAAGGGCACATCTAGTGCGGGCTGCTGAAGTGAAGAAGGCCATTGAATGGATTATGTGCAACCTTGACGGTCACTATGCAGCCGATTCTGTTGCTGCAGCAATTGCAGTTGGTGCCGCAGCTGCAGAAACAGCTCAAGAGAGTGATGGTCAAGGTGGCTTGCTTATGCCATATTCACCTCAGAGGGTTGCTACAAAGGCCCTTTCAATTCAAGGGAAGGCAAATGATGCAGCAAGTCCTTCAAACATCTCTAAGAATTTTAGAGCTGATGTATTACGAGAAAACACAGAAGCTGTTGTTGCAGCATGTGATGCTGCTCATGGAAGATGGGCAAAGCTACTCGGGGTTCGTGCTCTTCTTCATCCTAAGTTGAGATTGCAGGAGTTTTTGAGCATATATAACATCACACAAAACTTTATAACAGCTACGGAGAAGATAGGGGGAAGGCCAGGATTTAGCATCAGGGGAACACTACAGTCACAGGCCAAAGCCTTCATGGATTTTCAGCATGAATCTCGAATGGCAAAAATTAAAGCTGTTCTTGACCAAGAAACGTGGGTGGAAGTGGATGTTCCTGATGAATTTCAGGTCATTGTCACTTCACTATTTAGTTCTGAATCATTGGTAACCGAGAACCTGGATACTGTCCAAGATAATACGGAAACAAGCTACAATGAGGTGGCCACACCCAGTAATAGTTCACATGCAGCAGATACATCCGCAGGTATTACTGCAAAGGAGATGCCAAATTCTGATGGAACTGAGAAAAATAAGGCTGATGTTGCAAACTCTGTTGCTCAAAATAACCACAGCAACGTGAAGGAGCGAGGAAAATCAACCTCTCAAACCCTTTTCTACAAAGGTGTTGGTTTTCACATGGTAAACTGTGGCTTGATATTGATGAAGATGTTGTCAGAGTACATTGATATGAACAACTTTTTTCCAGCACTGTCCTCTGAAGTTGTTCATCGTATTGTAGAAATTTTAAAATTTTTCAATACAAGAACGTGTCAGCTTGTTCTTGGTGCCGGTGCCATGCAGGTATCCGGTTTAAAGTCTATCACTTCTAAACACTTGGCCTTGGCAAGTCAAGTCATCAGTTTTACATATGCTATTATTCCTGAAATCAGGCAAATTCTTTTTTTAAAAGTGCCAGAGACGAGAAAAGCATTGTTGTTATCTGAAATTGATCGAGTAGCTCAGGATTATAAGGTTCATAGAGATGAAATACACACCAAACTAGTTCAGATCATGAGAGAAAGGTTATTGGTTCATCTACGCGGGTTGCCTCAAATTGTAGAGAGCTGGAATAGACCTGAAGAGGCTGACCCACAGCCCAGCCAATTTGCTCGATCCCTTACCAAGGAAGTTGGATACTTGCAACGTGTTCTAACTCGAACTTTACATGAGGTCGATGTTCAAGCGATTTTCAGGCAAGTGATTTTGGTCTTCCATTCACAAATATCAGAGGCATTTTCACGGTTAGAGATCAGCACACCACAAGCCAAGGACAGGCTGCGCCGGGATGTCACACACATTCTTGGATGCATTCGTTCTTTGCCGTCTGATAAAATGAGCGAATCTAGTATTCCAAACTGGGGTCAGCTAGATGAATTCTTGGTGCAGAGATTTGGCGCGGAAGCTGGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000149 GO:0000938 GO:0001894 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005794 GO:0005802 GO:0006810 GO:0006892 GO:0006896 GO:0006996 GO:0007010 GO:0007034 GO:0007041 GO:0008150 GO:0009507 GO:0009536 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016192 GO:0016197 GO:0016482 GO:0019905 GO:0031090 GO:0031410 GO:0031974 GO:0031981 GO:0031982 GO:0031984 GO:0032501 GO:0032588 GO:0032991 GO:0040008 GO:0042147 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044431 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045103 GO:0045104 GO:0046907 GO:0048193 GO:0048471 GO:0048871 GO:0048872 GO:0048873 GO:0050789 GO:0050879 GO:0050881 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0060052 GO:0060249 GO:0065007 GO:0065008 GO:0070013 GO:0071840 GO:0097708 GO:0098588 GO:0098791 GO:0099023

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Vps54

Protein Analysis

1010

Amino Acids

111.26

Weight (kDa)

5.64

Isoelectric Point (pI)

41.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps54	PF07928	751 - 881	3.7e-47	Vps54-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005460)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19490 AT4G19490
fragaria_vesca	FvH4_6g01010 FvH4_6g01010 FvH4_6g01010
malus_domestica	MD04G1237800.v1.1 MD12G1256000.v1.1
prunus_persica	Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1 Prupe.6G356900_v2.0.a1
pyrus_communis	pycom04g21100 pycom12g23480
rosa_chinensis	RchiOBHm_Chr3g0448451
rosa_laevigata	RLG00000000687 RLG00000025905
rosa_multiflora	Rmu_sc0003308.1_g000012 Rmu_sc0011979.1_g000005
rosa_roxburghii	Rroxscaffold_6G00426680
rosa_rugosa	Rorug02G0612500 Rorug02G0612600 Rorug02G0612600 Rorug07G0334800
rosa_samantha	Rh3AG012000 Rh3BG011800 Rh3CG011500 Rh3DG012200 Rh7BG461900
rosa_wichuraiana	Rw3G000930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1007, 1799, 2589
AasI	GACNNNNNNGTC	1 cut(s)	614
AatII	GACGTC	2 cut(s)	357, 843
Acc16I	TGCGCA	1 cut(s)	697
Acc36I	ACCTGC	2 cut(s)	2093, 2410
AccB1I	GGYRCC	4 cut(s)	449, 1493, 2405, 2411
AccB7I	CCANNNNNTGG	1 cut(s)	1428
AccI	GTMKAC	1 cut(s)	889
AccII	CGCG	4 cut(s)	392, 1246, 2661, 3017
AciI	CCGC	9 cut(s)	198, 212, 310, 923, 1399, 1497, 2100, 2661, 3017
AclWI	GGATC	4 cut(s)	87, 258, 921, 2727
AcoI	YGGCCR	2 cut(s)	370, 2061
AcsI	RAATTY	8 cut(s)	218, 1636, 1961, 2128, 2361, 2369, 2514, 2993
AcuI	CTGAAG	3 cut(s)	1428, 2358, 2718
AcvI	CACGTG	1 cut(s)	334
AcyI	GRCGYC	6 cut(s)	66, 165, 252, 354, 450, 840
AdeI	CACNNNGTG	1 cut(s)	550
AfaI	GTAC	1 cut(s)	2300
AfiI	CCNNNNNNNGG	8 cut(s)	174, 316, 403, 459, 1428, 2743, 2892, 2978
AflIII	ACRYGT	2 cut(s)	2387, 2764
AhdI	GACNNNNNGTC	1 cut(s)	1195
AhlI	ACTAGT	1 cut(s)	2620
AjiI	CACGTC	1 cut(s)	532
AjnI	CCWGG	5 cut(s)	173, 495, 725, 1828, 2016
AloI	GAACNNNNNNTCC	2 cut(s)	77, 109
Alw21I	GWGCWC	2 cut(s)	654, 1735
Alw26I	GTCTC	5 cut(s)	298, 490, 653, 867, 2531
AlwI	GGATC	4 cut(s)	87, 258, 921, 2727
AlwNI	CAGNNNCTG	1 cut(s)	1673
Ama87I	CYCGRG	1 cut(s)	1721
AoxI	GGCC	7 cut(s)	370, 1419, 1578, 1826, 1863, 2061, 2457
ApoI	RAATTY	8 cut(s)	218, 1636, 1961, 2128, 2361, 2369, 2514, 2993
ArsI	GACNNNNNNTTYG	4 cut(s)	230, 262, 372, 404
Asp700I	GAANNNNTTC	1 cut(s)	2343
AspLEI	GCGC	7 cut(s)	415, 452, 592, 698, 1307, 2901, 3017
AspS9I	GGNCC	2 cut(s)	189, 1579
AsuC2I	CCSGG	4 cut(s)	75, 401, 1272, 2903
AsuHPI	GGTGA	4 cut(s)	562, 896, 1073, 1548
AvaI	CYCGRG	1 cut(s)	1721
AvaII	GGWCC	1 cut(s)	189
AxyI	CCTNAGG	1 cut(s)	908
BaeGI	GKGCMC	1 cut(s)	1390
BaeI	ACNNNNGTAYC	2 cut(s)	987, 1020
BalI	TGGCCA	2 cut(s)	372, 2063
BanI	GGYRCC	4 cut(s)	449, 1493, 2405, 2411
BanII	GRGCYC	1 cut(s)	654
BauI	CACGAG	1 cut(s)	230
BbrPI	CACGTG	1 cut(s)	334
BbsI	GAAGAC	2 cut(s)	1100, 2821
Bbv12I	GWGCWC	2 cut(s)	654, 1735
BceAI	ACGGC	2 cut(s)	239, 2926
BcgI	CGANNNNNNTGC	4 cut(s)	1454, 1488, 1877, 1911
BciT130I	CCWGG	5 cut(s)	175, 497, 727, 1830, 2018
BciVI	GTATCC	3 cut(s)	2014, 2434, 2747
BcnI	CCSGG	4 cut(s)	75, 401, 1272, 2903
BcoDI	GTCTC	5 cut(s)	298, 490, 653, 867, 2531
BcuI	ACTAGT	1 cut(s)	2620
BfaI	CTAG	5 cut(s)	1038, 1394, 2621, 2964, 2987
BfmI	CTRYAG	7 cut(s)	503, 746, 816, 957, 1476, 1503, 1853
BfoI	RGCGCY	2 cut(s)	416, 453
BfuAI	ACCTGC	2 cut(s)	2093, 2410
BfuI	GTATCC	3 cut(s)	2014, 2434, 2747
BglII	AGATCT	1 cut(s)	558
Bme1390I	CCNGG	9 cut(s)	75, 175, 401, 497, 727, 1272, 1830, 2018, 2903
Bme18I	GGWCC	1 cut(s)	189
BmeRI	GACNNNNNGTC	1 cut(s)	1195
BmeT110I	CYCGRG	1 cut(s)	1721
BmgBI	CACGTC	1 cut(s)	532
BmgT120I	GGNCC	2 cut(s)	189, 1579
BmiI	GGNNCC	7 cut(s)	408, 451, 832, 1331, 1495, 2407, 2413
BmrFI	CCNGG	9 cut(s)	75, 175, 401, 497, 727, 1272, 1830, 2018, 2903
BmrI	ACTGGG	2 cut(s)	2064, 2986
BmsI	GCATC	9 cut(s)	976, 1043, 1279, 1316, 1597, 1681, 1848, 2112, 2915
BmuI	ACTGGG	2 cut(s)	2064, 2986
BpiI	GAAGAC	2 cut(s)	1100, 2821
BpmI	CTGGAG	4 cut(s)	623, 849, 1011, 1274
Bpu10I	CCTNAGC	1 cut(s)	2580
BpuEI	CTTGAG	3 cut(s)	461, 638, 1500
BpuMI	CCSGG	4 cut(s)	75, 401, 1272, 2903
BsaAI	YACGTR	1 cut(s)	334
BsaBI	GATNNNNATC	1 cut(s)	56
BsaHI	GRCGYC	6 cut(s)	66, 165, 252, 354, 450, 840
BsaJI	CCNNGG	8 cut(s)	174, 373, 496, 834, 1271, 2460, 2742, 2886
BsaWI	WCCGGW	2 cut(s)	459, 2426
BsaXI	ACNNNNNCTCC	6 cut(s)	155, 185, 272, 302, 500, 530
Bsc4I	CCNNNNNNNGG	8 cut(s)	174, 316, 403, 459, 1428, 2743, 2892, 2978
Bse118I	RCCGGY	1 cut(s)	2408
Bse1I	ACTGG	4 cut(s)	889, 1257, 2070, 2981
Bse21I	CCTNAGG	1 cut(s)	908
Bse3DI	GCAATG	2 cut(s)	40, 892
Bse8I	GATNNNNATC	1 cut(s)	56
BseBI	CCWGG	5 cut(s)	175, 497, 727, 1830, 2018
BseDI	CCNNGG	8 cut(s)	174, 373, 496, 834, 1271, 2460, 2742, 2886
BseJI	GATNNNNATC	1 cut(s)	56
BseLI	CCNNNNNNNGG	8 cut(s)	174, 316, 403, 459, 1428, 2743, 2892, 2978
BseMI	GCAATG	2 cut(s)	40, 892
BseMII	CTCAG	4 cut(s)	501, 1574, 2133, 2594
BseNI	ACTGG	4 cut(s)	889, 1257, 2070, 2981
BseRI	GAGGAG	3 cut(s)	160, 184, 1342
BseSI	GKGCMC	1 cut(s)	1390
BseYI	CCCAGC	1 cut(s)	2717
BsgI	GTGCAG	2 cut(s)	655, 3023
Bsh1236I	CGCG	4 cut(s)	392, 1246, 2661, 3017
Bsh1285I	CGRYCG	1 cut(s)	464
BshFI	GGCC	7 cut(s)	372, 1421, 1580, 1828, 1865, 2063, 2459
BshNI	GGYRCC	4 cut(s)	449, 1493, 2405, 2411
BsiEI	CGRYCG	1 cut(s)	464
BsiHKAI	GWGCWC	2 cut(s)	654, 1735
BsiHKCI	CYCGRG	1 cut(s)	1721
BsiSI	CCGG	8 cut(s)	75, 400, 410, 460, 1271, 2409, 2427, 2902
BslI	CCNNNNNNNGG	8 cut(s)	174, 316, 403, 459, 1428, 2743, 2892, 2978
BsmAI	GTCTC	5 cut(s)	298, 490, 653, 867, 2531
BsmBI	CGTCTC	5 cut(s)	298, 490, 653, 867, 2531
BsmI	GAATGC	2 cut(s)	948, 2928
BsnI	GGCC	7 cut(s)	372, 1421, 1580, 1828, 1865, 2063, 2459
BsoBI	CYCGRG	1 cut(s)	1721
Bsp1286I	GDGCHC	3 cut(s)	654, 1390, 1735
Bsp68I	TCGCGA	1 cut(s)	1246
BspACI	CCGC	9 cut(s)	198, 212, 310, 923, 1399, 1497, 2100, 2661, 3017
BspANI	GGCC	7 cut(s)	372, 1421, 1580, 1828, 1865, 2063, 2459
BspCNI	CTCAG	4 cut(s)	500, 1573, 2134, 2593
BspFNI	CGCG	4 cut(s)	392, 1246, 2661, 3017
BspHI	TCATGA	2 cut(s)	709, 2631
BspLI	GGNNCC	7 cut(s)	408, 451, 832, 1331, 1495, 2407, 2413
BspMAI	CTGCAG	6 cut(s)	507, 750, 820, 961, 1480, 1507
BspMI	ACCTGC	2 cut(s)	2093, 2410
BspPI	GGATC	4 cut(s)	87, 258, 921, 2727
BspQI	GCTCTTC	1 cut(s)	1740
BspT107I	GGYRCC	4 cut(s)	449, 1493, 2405, 2411
BsrDI	GCAATG	2 cut(s)	40, 892
BsrFI	RCCGGY	1 cut(s)	2408
BsrI	ACTGG	4 cut(s)	889, 1257, 2070, 2981
BssAI	RCCGGY	1 cut(s)	2408
BssECI	CCNNGG	8 cut(s)	174, 373, 496, 834, 1271, 2460, 2742, 2886
BssNI	GRCGYC	6 cut(s)	66, 165, 252, 354, 450, 840
BssSI	CACGAG	1 cut(s)	230
BssT1I	CCWWGG	5 cut(s)	373, 834, 2460, 2742, 2886
Bst2BI	CACGAG	1 cut(s)	230
Bst2UI	CCWGG	5 cut(s)	175, 497, 727, 1830, 2018
Bst4CI	ACNGT	6 cut(s)	1451, 1857, 2026, 2267, 2331, 2862
Bst6I	CTCTTC	2 cut(s)	1740, 2694
BstACI	GRCGYC	6 cut(s)	66, 165, 252, 354, 450, 840
BstAPI	GCANNNNNTGC	6 cut(s)	824, 1313, 1484, 1493, 1607, 1691
BstBAI	YACGTR	1 cut(s)	334
BstC8I	GCNNGC	6 cut(s)	394, 469, 972, 1318, 1401, 1541
BstDEI	CTNAG	8 cut(s)	487, 908, 1133, 1560, 1632, 1746, 2142, 2580
BstEII	GGTNACC	1 cut(s)	2005
BstENI	CCTNNNNNAGG	2 cut(s)	401, 2741
BstFNI	CGCG	4 cut(s)	392, 1246, 2661, 3017
BstH2I	RGCGCY	2 cut(s)	416, 453
BstHHI	GCGC	7 cut(s)	415, 452, 592, 698, 1307, 2901, 3017
BstMAI	GTCTC	5 cut(s)	298, 490, 653, 867, 2531
BstMCI	CGRYCG	1 cut(s)	464
BstNI	CCWGG	5 cut(s)	175, 497, 727, 1830, 2018
BstNSI	RCATGY	2 cut(s)	1689, 2087
BstPI	GGTNACC	1 cut(s)	2005
BstSCI	CCNGG	9 cut(s)	73, 173, 399, 495, 725, 1270, 1828, 2016, 2901
BstSFI	CTRYAG	7 cut(s)	503, 746, 816, 957, 1476, 1503, 1853
BstSLI	GKGCMC	1 cut(s)	1390
BstUI	CGCG	4 cut(s)	392, 1246, 2661, 3017
BstV2I	GAAGAC	2 cut(s)	1100, 2821
BstX2I	RGATCY	1 cut(s)	558
BstYI	RGATCY	1 cut(s)	558
Bsu36I	CCTNAGG	1 cut(s)	908
BsuI	GTATCC	3 cut(s)	2014, 2434, 2747
BsuRI	GGCC	7 cut(s)	372, 1421, 1580, 1828, 1865, 2063, 2459
BtrI	CACGTC	1 cut(s)	532
BtsIMutI	CAGTG	2 cut(s)	1250, 2327
BtuMI	TCGCGA	1 cut(s)	1246
BveI	ACCTGC	2 cut(s)	2093, 2410
Cac8I	GCNNGC	6 cut(s)	394, 469, 972, 1318, 1401, 1541
CaiI	CAGNNNCTG	1 cut(s)	1673
CciI	TCATGA	2 cut(s)	709, 2631
CfoI	GCGC	7 cut(s)	415, 452, 592, 698, 1307, 2901, 3017
Cfr10I	RCCGGY	1 cut(s)	2408
Cfr13I	GGNCC	2 cut(s)	189, 1579
CseI	GACGC	4 cut(s)	74, 173, 234, 260
Csp6I	GTAC	1 cut(s)	2299
CspCI	CAANNNNNGTGG	2 cut(s)	1916, 1951
CviQI	GTAC	1 cut(s)	2299
DdeI	CTNAG	8 cut(s)	487, 908, 1133, 1560, 1632, 1746, 2142, 2580
DinI	GGCGCC	1 cut(s)	451
DraI	TTTAAA	3 cut(s)	2367, 2433, 2526
DraIII	CACNNNGTG	1 cut(s)	550
DrdI	GACNNNNNNGTC	1 cut(s)	614
DriI	GACNNNNNGTC	1 cut(s)	1195
DseDI	GACNNNNNNGTC	1 cut(s)	614
EaeI	YGGCCR	2 cut(s)	370, 2061
Eam1104I	CTCTTC	2 cut(s)	1740, 2694
Eam1105I	GACNNNNNGTC	1 cut(s)	1195
EarI	CTCTTC	2 cut(s)	1740, 2694
EciI	GGCGGA	1 cut(s)	187
Ecl136II	GAGCTC	1 cut(s)	652
Eco130I	CCWWGG	5 cut(s)	373, 834, 2460, 2742, 2886
Eco24I	GRGCYC	1 cut(s)	654
Eco47I	GGWCC	1 cut(s)	189
Eco53kI	GAGCTC	1 cut(s)	652
Eco57I	CTGAAG	3 cut(s)	1428, 2358, 2718
Eco72I	CACGTG	1 cut(s)	334
Eco81I	CCTNAGG	1 cut(s)	908
Eco88I	CYCGRG	1 cut(s)	1721
Eco91I	GGTNACC	1 cut(s)	2005
EcoICRI	GAGCTC	1 cut(s)	652
EcoNI	CCTNNNNNAGG	2 cut(s)	401, 2741
EcoO109I	RGGNCCY	1 cut(s)	1579
EcoO65I	GGTNACC	1 cut(s)	2005
EcoRI	GAATTC	2 cut(s)	218, 2993
EcoRII	CCWGG	5 cut(s)	173, 495, 725, 1828, 2016
EcoT14I	CCWWGG	5 cut(s)	373, 834, 2460, 2742, 2886
EcoT22I	ATGCAT	1 cut(s)	2930
EcoT38I	GRGCYC	1 cut(s)	654
EgeI	GGCGCC	1 cut(s)	451
EheI	GGCGCC	1 cut(s)	451
ErhI	CCWWGG	5 cut(s)	373, 834, 2460, 2742, 2886
Esp3I	CGTCTC	5 cut(s)	298, 490, 653, 867, 2531
FalI	AAGNNNNNCTT	6 cut(s)	416, 448, 1092, 1124, 1905, 1937
FauI	CCCGC	3 cut(s)	219, 1392, 2654
FauNDI	CATATG	1 cut(s)	2488
FblI	GTMKAC	1 cut(s)	889
FriOI	GRGCYC	1 cut(s)	654
FspBI	CTAG	5 cut(s)	1038, 1394, 2621, 2964, 2987
FspI	TGCGCA	1 cut(s)	697
GlaI	GCGC	7 cut(s)	414, 451, 591, 697, 1306, 2900, 3016
GsaI	CCCAGC	1 cut(s)	2721
GsuI	CTGGAG	4 cut(s)	623, 849, 1011, 1274
HaeII	RGCGCY	2 cut(s)	416, 453
HaeIII	GGCC	7 cut(s)	372, 1421, 1580, 1828, 1865, 2063, 2459
HapII	CCGG	8 cut(s)	75, 400, 410, 460, 1271, 2409, 2427, 2902
HgaI	GACGC	4 cut(s)	74, 173, 234, 260
HhaI	GCGC	7 cut(s)	415, 452, 592, 698, 1307, 2901, 3017
Hin1I	GRCGYC	6 cut(s)	66, 165, 252, 354, 450, 840
Hin6I	GCGC	7 cut(s)	413, 450, 590, 696, 1305, 2899, 3015
HinP1I	GCGC	7 cut(s)	413, 450, 590, 696, 1305, 2899, 3015
HindIII	AAGCTT	1 cut(s)	1345
HpaII	CCGG	8 cut(s)	75, 400, 410, 460, 1271, 2409, 2427, 2902
HphI	GGTGA	4 cut(s)	562, 896, 1073, 1548
Hpy166II	GTNNAC	4 cut(s)	477, 890, 2081, 2263
Hpy8I	GTNNAC	4 cut(s)	477, 890, 2081, 2263
Hpy99I	CGWCG	6 cut(s)	167, 254, 356, 359, 392, 629
HpyCH4III	ACNGT	6 cut(s)	1451, 1857, 2026, 2267, 2331, 2862
HpyF3I	CTNAG	8 cut(s)	487, 908, 1133, 1560, 1632, 1746, 2142, 2580
Hsp92I	GRCGYC	6 cut(s)	66, 165, 252, 354, 450, 840
HspAI	GCGC	7 cut(s)	413, 450, 590, 696, 1305, 2899, 3015
KasI	GGCGCC	1 cut(s)	449
LguI	GCTCTTC	1 cut(s)	1740
LmnI	GCTCC	7 cut(s)	538, 729, 830, 1152, 1276, 1329, 2203
LweI	GCATC	9 cut(s)	976, 1043, 1279, 1316, 1597, 1681, 1848, 2112, 2915
MaeI	CTAG	5 cut(s)	1038, 1394, 2621, 2964, 2987
MaeIII	GTNAC	8 cut(s)	256, 841, 1172, 1451, 1857, 1975, 2005, 2908
MfeI	CAATTG	2 cut(s)	930, 1482
MflI	RGATCY	1 cut(s)	558
MhlI	GDGCHC	3 cut(s)	654, 1390, 1735
MlsI	TGGCCA	2 cut(s)	372, 2063
MluNI	TGGCCA	2 cut(s)	372, 2063
Mly113I	GGCGCC	1 cut(s)	450
MlyI	GAGTC	1 cut(s)	1265
MmeI	TCCRAC	9 cut(s)	66, 132, 186, 273, 375, 597, 620, 1053, 2731
Mox20I	TGGCCA	2 cut(s)	372, 2063
Mph1103I	ATGCAT	1 cut(s)	2930
MroXI	GAANNNNTTC	1 cut(s)	2343
MscI	TGGCCA	2 cut(s)	372, 2063
MseI	TTAA	9 cut(s)	777, 798, 1068, 1361, 1911, 2366, 2432, 2525, 3028
MslI	CAYNNNNRTG	2 cut(s)	2306, 2601
Msp20I	TGGCCA	2 cut(s)	372, 2063
MspA1I	CMGCKG	3 cut(s)	601, 1187, 1502
MspI	CCGG	8 cut(s)	75, 400, 410, 460, 1271, 2409, 2427, 2902
MspR9I	CCNGG	9 cut(s)	75, 175, 401, 497, 727, 1272, 1830, 2018, 2903
MunI	CAATTG	2 cut(s)	930, 1482
Mva1269I	GAATGC	2 cut(s)	948, 2928
MvaI	CCWGG	5 cut(s)	175, 497, 727, 1830, 2018
MvnI	CGCG	4 cut(s)	392, 1246, 2661, 3017
NarI	GGCGCC	1 cut(s)	450
NciI	CCSGG	4 cut(s)	75, 401, 1272, 2903
NdeI	CATATG	1 cut(s)	2488
NlaIV	GGNNCC	7 cut(s)	408, 451, 832, 1331, 1495, 2407, 2413
NmuCI	GTSAC	7 cut(s)	256, 841, 1172, 1451, 1857, 1975, 2908
NruI	TCGCGA	1 cut(s)	1246
NsbI	TGCGCA	1 cut(s)	697
NsiI	ATGCAT	1 cut(s)	2930
NspI	RCATGY	2 cut(s)	1689, 2087
PagI	TCATGA	2 cut(s)	709, 2631
PciSI	GCTCTTC	1 cut(s)	1740
PcsI	WCGNNNNNNNCGW	3 cut(s)	228, 470, 687
PctI	GAATGC	2 cut(s)	948, 2928
PdmI	GAANNNNTTC	1 cut(s)	2343
PfeI	GAWTC	9 cut(s)	359, 643, 664, 706, 935, 1466, 1892, 1997, 2960
PflMI	CCANNNNNTGG	1 cut(s)	1428
PfoI	TCCNGGA	1 cut(s)	399
PleI	GAGTC	1 cut(s)	1264
PluTI	GGCGCC	1 cut(s)	453
PmaCI	CACGTG	1 cut(s)	334
PmlI	CACGTG	1 cut(s)	334
PpsI	GAGTC	1 cut(s)	1264
Ppu21I	YACGTR	1 cut(s)	334
PsiI	TTATAA	3 cut(s)	1007, 1799, 2589
Psp124BI	GAGCTC	1 cut(s)	654
Psp6I	CCWGG	5 cut(s)	173, 495, 725, 1828, 2016
PspCI	CACGTG	1 cut(s)	334
PspEI	GGTNACC	1 cut(s)	2005
PspFI	CCCAGC	1 cut(s)	2717
PspGI	CCWGG	5 cut(s)	173, 495, 725, 1828, 2016
PspN4I	GGNNCC	7 cut(s)	408, 451, 832, 1331, 1495, 2407, 2413
PspPI	GGNCC	2 cut(s)	189, 1579
PsrI	GAACNNNNNNTAC	1 cut(s)	34
PstI	CTGCAG	6 cut(s)	507, 750, 820, 961, 1480, 1507
PstNI	CAGNNNCTG	1 cut(s)	1673
PsuI	RGATCY	1 cut(s)	558
PvuII	CAGCTG	3 cut(s)	601, 1187, 1502
RruI	TCGCGA	1 cut(s)	1246
RsaI	GTAC	1 cut(s)	2300
RsaNI	GTAC	1 cut(s)	2299
RseI	CAYNNNNRTG	2 cut(s)	2306, 2601
SacI	GAGCTC	1 cut(s)	654
SapI	GCTCTTC	1 cut(s)	1740
SaqAI	TTAA	9 cut(s)	777, 798, 1068, 1361, 1911, 2366, 2432, 2525, 3028
Sau96I	GGNCC	2 cut(s)	189, 1579
SchI	GAGTC	1 cut(s)	1265
ScrFI	CCNGG	9 cut(s)	75, 175, 401, 497, 727, 1272, 1830, 2018, 2903
SduI	GDGCHC	3 cut(s)	654, 1390, 1735
SfaNI	GCATC	9 cut(s)	976, 1043, 1279, 1316, 1597, 1681, 1848, 2112, 2915
SfcI	CTRYAG	7 cut(s)	503, 746, 816, 957, 1476, 1503, 1853
SfoI	GGCGCC	1 cut(s)	451
SinI	GGWCC	1 cut(s)	189
SmiMI	CAYNNNNRTG	2 cut(s)	2306, 2601
SmlI	CTYRAG	3 cut(s)	440, 653, 1515
SmoI	CTYRAG	3 cut(s)	440, 653, 1515
SpeI	ACTAGT	1 cut(s)	2620
SsiI	CCGC	9 cut(s)	198, 212, 310, 923, 1399, 1497, 2100, 2661, 3017
SspDI	GGCGCC	1 cut(s)	449
SspMI	CTAG	5 cut(s)	1038, 1394, 2621, 2964, 2987
SstI	GAGCTC	1 cut(s)	654
StyD4I	CCNGG	9 cut(s)	73, 173, 399, 495, 725, 1270, 1828, 2016, 2901
StyI	CCWWGG	5 cut(s)	373, 834, 2460, 2742, 2886
TaaI	ACNGT	6 cut(s)	1451, 1857, 2026, 2267, 2331, 2862
TatI	WGTACW	1 cut(s)	2298
TauI	GCSGC	2 cut(s)	926, 1499
TfiI	GAWTC	9 cut(s)	359, 643, 664, 706, 935, 1466, 1892, 1997, 2960
Tru1I	TTAA	9 cut(s)	777, 798, 1068, 1361, 1911, 2366, 2432, 2525, 3028
Tru9I	TTAA	9 cut(s)	777, 798, 1068, 1361, 1911, 2366, 2432, 2525, 3028
TscAI	CASTG	2 cut(s)	1257, 2334
TseFI	GTSAC	7 cut(s)	256, 841, 1172, 1451, 1857, 1975, 2908
Tsp45I	GTSAC	7 cut(s)	256, 841, 1172, 1451, 1857, 1975, 2908
TspGWI	ACGGA	4 cut(s)	193, 280, 1823, 2054
TspRI	CASTG	2 cut(s)	1257, 2334
Van91I	CCANNNNNTGG	1 cut(s)	1428
VpaK11BI	GGWCC	1 cut(s)	189
XagI	CCTNNNNNAGG	2 cut(s)	401, 2741
XapI	RAATTY	8 cut(s)	218, 1636, 1961, 2128, 2361, 2369, 2514, 2993
XceI	RCATGY	2 cut(s)	1689, 2087
XcmI	CCANNNNNNNNNTGG	2 cut(s)	49, 1873
XmiI	GTMKAC	1 cut(s)	889
XmnI	GAANNNNTTC	1 cut(s)	2343
XspI	CTAG	5 cut(s)	1038, 1394, 2621, 2964, 2987
ZraI	GACGTC	2 cut(s)	355, 841
Zsp2I	ATGCAT	1 cut(s)	2930

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.