AT4G20780

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013009
Physical Location & Seq
Reverse (-)
11133095 .. 11134075
981 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20780.1

Sequence Viewer

Length: 576 bp
ATGGAGAGTAACAACAACGAGAAGAAGAAAGTTGCAAGACAATCATCATCCTTCAGACTACGTAGCCCAAGCCTAAACGCACTTCGTCTCCAACGAATCTTCGACTTATTCGACAAAAACGGCGACGGATTCATAACCGTCGAAGAGCTAAGCCAAGCTCTAACACGTCTTGGTCTCAACGCTGATCTCTCCGATCTCAAATCCACCGTTGAATCATACATCCAACCTGGTAACACTGGTCTCAATTTCGATGATTTCTCTTCTCTTCACAAAACACTCGACGATTCTTTCTTCGGTGGAGCTTGTGGAGGAGGAGAAAACGAAGATGATCCTTCTTCCGCAGCTGAAAATGAATCGGATCTCGCCGAGGCGTTTAAGGTGTTTGATGAGAACGGTGATGGATTCATCTCCGCTAGAGAACTGCAAACTGTTCTGAAGAAACTTGGTTTACCTGAAGGTGGAGAAATGGAGAGAGTGGAGAAGATGATCGTTTCCGTTGACCGGAATCAAGATGGTCGCGTTGACTTTTTTGAATTCAAGAACATGATGCGTACTGTTGTCATCCCTTCTTCTTGA

Protein Analysis

191

Amino Acids

21.15

Weight (kDa)

4.59

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 29 - 87 5.5e-09 EF-hand domain pair
EF-hand_6 PF13405 29 - 58 3.3e-09 EF-hand domain
EF-hand_1 PF00036 30 - 57 1.2e-08 EF hand domain
EF-hand_5 PF13202 31 - 52 6.2e-07 EF hand
EF-hand_7 PF13499 118 - 184 2.7e-15 EF-hand domain pair
EF-hand_1 PF00036 121 - 148 1.5e-07 EF hand domain
EF-hand_6 PF13405 121 - 149 6.8e-08 EF-hand domain
EF-hand_5 PF13202 122 - 145 3.2e-07 EF hand
EF-hand_1 PF00036 159 - 185 4.3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20780 AT5G44460
fragaria_vesca FvH4_6g09300
malus_domestica MD04G1178900.v1.1 MD07G1157000.v1.1 MD12G1194200.v1.1
prunus_persica Prupe.6G298900_v2.0.a1
pyrus_communis pycom04g15820 pycom12g18040
rosa_chinensis RchiOBHm_Chr3g0459681
rosa_laevigata RLG00000025067
rosa_multiflora Rmu_sc0000240.1_g000021
rosa_roxburghii Rroxscaffold_6G00420080
rosa_rugosa Rorug03G0037400
rosa_samantha Rh3AG096000 Rh3BG098600 Rh3CG100600 Rh3DG100000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 519
AciI CCGC 2 cut(s) 339, 411
AclWI GGATC 2 cut(s) 323, 366
AcsI RAATTY 1 cut(s) 533
AcuI CTGAAG 3 cut(s) 37, 455, 474
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 2 cut(s) 458, 501
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 3 cut(s) 212, 533, 538
AjiI CACGTC 1 cut(s) 167
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 4 cut(s) 148, 158, 302, 344
AluI AGCT 4 cut(s) 148, 158, 302, 344
Alw26I GTCTC 3 cut(s) 92, 179, 245
AlwI GGATC 2 cut(s) 323, 366
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 1 cut(s) 533
AsuHPI GGTGA 1 cut(s) 407
BbvI GCAGC 1 cut(s) 353
BccI CCATC 2 cut(s) 392, 506
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 228
BcoDI GTCTC 3 cut(s) 92, 179, 245
BfaI CTAG 1 cut(s) 414
BisI GCNGC 1 cut(s) 342
BlpI GCTNAGC 1 cut(s) 149
BlsI GCNGC 1 cut(s) 343
Bme1390I CCNGG 1 cut(s) 228
BmgBI CACGTC 1 cut(s) 167
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 537
Bpu1102I GCTNAGC 1 cut(s) 149
BsaAI YACGTR 1 cut(s) 62
BsaI GGTCTC 2 cut(s) 179, 245
BsaJI CCNNGG 1 cut(s) 366
BsaWI WCCGGW 1 cut(s) 501
BsaXI ACNNNNNCTCC 2 cut(s) 72, 102
Bsc4I CCNNNNNNNGG 2 cut(s) 458, 501
Bse1I ACTGG 1 cut(s) 241
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 3 cut(s) 47, 219, 561
BseLI CCNNNNNNNGG 2 cut(s) 458, 501
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 2 cut(s) 324, 327
BseXI GCAGC 1 cut(s) 353
Bsh1236I CGCG 1 cut(s) 519
BsiSI CCGG 1 cut(s) 502
BslI CCNNNNNNNGG 2 cut(s) 458, 501
BsmAI GTCTC 3 cut(s) 92, 179, 245
BsmBI CGTCTC 1 cut(s) 92
Bso31I GGTCTC 2 cut(s) 179, 245
Bsp143I GATC 5 cut(s) 184, 193, 328, 358, 486
Bsp1720I GCTNAGC 1 cut(s) 149
BspACI CCGC 2 cut(s) 339, 411
BspFNI CGCG 1 cut(s) 519
BspPI GGATC 2 cut(s) 323, 366
BspQI GCTCTTC 1 cut(s) 138
BspTNI GGTCTC 2 cut(s) 179, 245
BsrI ACTGG 1 cut(s) 241
BssECI CCNNGG 1 cut(s) 366
BssMI GATC 5 cut(s) 184, 193, 328, 358, 486
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 5 cut(s) 139, 208, 395, 430, 556
Bst6I CTCTTC 3 cut(s) 138, 265, 270
BstBAI YACGTR 1 cut(s) 62
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 3 cut(s) 47, 219, 561
BstFNI CGCG 1 cut(s) 519
BstKTI GATC 5 cut(s) 187, 196, 331, 361, 489
BstMAI GTCTC 3 cut(s) 92, 179, 245
BstMBI GATC 5 cut(s) 184, 193, 328, 358, 486
BstNI CCWGG 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 226
BstSNI TACGTA 1 cut(s) 62
BstUI CGCG 1 cut(s) 519
BstV1I GCAGC 1 cut(s) 353
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BtrI CACGTC 1 cut(s) 167
BtsCI GGATG 3 cut(s) 47, 219, 561
BtsIMutI CAGTG 1 cut(s) 234
CsiI ACCWGGT 1 cut(s) 226
Csp6I GTAC 1 cut(s) 552
CviAII CATG 1 cut(s) 544
CviJI RGCY 7 cut(s) 66, 72, 148, 153, 158, 302, 344
CviKI_1 RGCY 7 cut(s) 66, 72, 148, 153, 158, 302, 344
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 1 cut(s) 149
DpnI GATC 5 cut(s) 186, 195, 330, 360, 488
DpnII GATC 5 cut(s) 184, 193, 328, 358, 486
Eam1104I CTCTTC 3 cut(s) 138, 265, 270
EarI CTCTTC 3 cut(s) 138, 265, 270
Eco105I TACGTA 1 cut(s) 62
Eco31I GGTCTC 2 cut(s) 179, 245
Eco57I CTGAAG 3 cut(s) 37, 455, 474
EcoRI GAATTC 1 cut(s) 533
EcoRII CCWGG 1 cut(s) 226
Esp3I CGTCTC 1 cut(s) 92
FaeI CATG 1 cut(s) 547
FaiI YATR 3 cut(s) 134, 217, 545
FatI CATG 1 cut(s) 543
Fnu4HI GCNGC 1 cut(s) 342
FokI GGATG 3 cut(s) 34, 206, 548
Fsp4HI GCNGC 1 cut(s) 342
FspBI CTAG 1 cut(s) 414
GluI GCNGC 1 cut(s) 342
HapII CCGG 1 cut(s) 502
Hin1II CATG 1 cut(s) 547
HincII GTYRAC 2 cut(s) 499, 523
HindII GTYRAC 2 cut(s) 499, 523
HinfI GANTC 7 cut(s) 96, 129, 212, 284, 353, 402, 505
HpaII CCGG 1 cut(s) 502
HphI GGTGA 1 cut(s) 407
Hpy166II GTNNAC 3 cut(s) 449, 499, 523
Hpy188I TCNGA 4 cut(s) 56, 193, 358, 435
Hpy188III TCNNGA 3 cut(s) 509, 538, 573
Hpy8I GTNNAC 3 cut(s) 449, 499, 523
Hpy99I CGWCG 3 cut(s) 128, 143, 284
HpyAV CCTTC 4 cut(s) 61, 342, 449, 576
HpyCH4III ACNGT 5 cut(s) 139, 208, 395, 430, 556
HpyCH4IV ACGT 2 cut(s) 61, 166
HpyCH4V TGCA 2 cut(s) 35, 424
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 2 cut(s) 61, 166
Hsp92II CATG 1 cut(s) 547
Kzo9I GATC 5 cut(s) 184, 193, 328, 358, 486
LguI GCTCTTC 1 cut(s) 138
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 5 cut(s) 213, 222, 240, 465, 515
Lsp1109I GCAGC 1 cut(s) 353
LweI GCATC 1 cut(s) 537
MabI ACCWGGT 1 cut(s) 226
MaeI CTAG 1 cut(s) 414
MaeII ACGT 2 cut(s) 61, 166
MaeIII GTNAC 2 cut(s) 8, 230
MalI GATC 5 cut(s) 186, 195, 330, 360, 488
MboI GATC 5 cut(s) 184, 193, 328, 358, 486
MflI RGATCY 1 cut(s) 358
MluCI AATT 2 cut(s) 244, 533
MmeI TCCRAC 2 cut(s) 115, 247
MnlI CCTC 3 cut(s) 302, 305, 361
MseI TTAA 1 cut(s) 375
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 1 cut(s) 502
MspR9I CCNGG 1 cut(s) 228
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 519
NdeII GATC 5 cut(s) 184, 193, 328, 358, 486
NlaIII CATG 1 cut(s) 547
NmeAIII GCCGAG 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 138
PcsI WCGNNNNNNNCGW 2 cut(s) 91, 108
PfeI GAWTC 7 cut(s) 96, 129, 212, 284, 353, 402, 505
PkrI GCNGC 1 cut(s) 343
Ppu21I YACGTR 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 226
PspGI CCWGG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 358
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
SapI GCTCTTC 1 cut(s) 138
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 5 cut(s) 184, 193, 328, 358, 486
ScrFI CCNGG 1 cut(s) 228
SexAI ACCWGGT 1 cut(s) 226
SfaNI GCATC 1 cut(s) 537
SnaBI TACGTA 1 cut(s) 62
Sse9I AATT 2 cut(s) 244, 533
SsiI CCGC 2 cut(s) 339, 411
SspMI CTAG 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 226
TaaI ACNGT 5 cut(s) 139, 208, 395, 430, 556
TaiI ACGT 2 cut(s) 64, 169
TaqI TCGA 5 cut(s) 102, 111, 141, 249, 279
TasI AATT 2 cut(s) 244, 533
TfiI GAWTC 7 cut(s) 96, 129, 212, 284, 353, 402, 505
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 1 cut(s) 241
TseI GCWGC 1 cut(s) 341
TspDTI ATGAA 3 cut(s) 121, 366, 394
TspGWI ACGGA 2 cut(s) 141, 484
TspRI CASTG 1 cut(s) 241
XapI RAATTY 1 cut(s) 533
XspI CTAG 1 cut(s) 414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.