pycom04g15820

Calcium-binding protein CML42-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013009
Physical Location & Seq
Reverse (-)
18582035 .. 18582664
630 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g15820.1

Sequence Viewer

Length: 630 bp
ATGGCCTCTACAGTTTCAGGGACTGGTGCTCCTTCGTTAAAACGGCCTTCGGCGTCGTTTCGCCTCCGCTGCACGAGTCTCAACTCCCTCCGCCTCCGCCGCATCTTTGATATGTTCGACAAGAACAAAGACGGCTTCATCACTGTCCACGAGATCAGCCAAGCCCTAGCACTTCTCGGGTTAGACACTGAAATCTCCGACCTCCATTCCACGATAAAATCCTTCATCCAACCCTCAAACGACGGCCTCAACTTCGACGACTTCCTCTCCCTCCACCAATCCCTCTATGACACCTATTTGGACAATAACAACAACGACGACGAGGTTGTTGCTTCCGCTGCTGAGGGAGCATCAACAGCGGCACGCGACGGAAACTCAAAGGAGAATATGTTGTCGCAAGAGGAGTCGGATCTTTCGGAGGCGTTCAAGGTGTTTGATGAGGACGGCGACGGGTACATATCGGCTAAAGAGTTGCAGACGGTTCTTGCCAAGCTTGGATTCCAGGAAGGCAATGAGATTGATAGAGTTCAGAAGATGATCACCTCCGTTGACCATAATCATGACGGTCTCGTCGACTTGTTCGAGTTTAAGCACATGATGCGCACTTCCGTTCTTGTTCCCTCCTCTTAA

Protein Analysis

210

Amino Acids

23.1

Weight (kDa)

4.65

Isoelectric Point (pI)

39.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 31 - 91 4.2e-08 EF-hand domain pair
EF-hand_6 PF13405 31 - 60 3.2e-08 EF-hand domain
EF-hand_1 PF00036 32 - 57 5.8e-07 EF hand domain
EF-hand_7 PF13499 135 - 201 9.9e-15 EF-hand domain pair
EF-hand_1 PF00036 138 - 165 5.3e-08 EF hand domain
EF-hand_6 PF13405 138 - 166 2.9e-08 EF-hand domain
EF-hand_5 PF13202 141 - 162 9.4e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20780 AT5G44460
fragaria_vesca FvH4_6g09300
malus_domestica MD04G1178900.v1.1 MD07G1157000.v1.1 MD12G1194200.v1.1
prunus_persica Prupe.6G298900_v2.0.a1
pyrus_communis pycom04g15820 pycom12g18040
rosa_chinensis RchiOBHm_Chr3g0459681
rosa_laevigata RLG00000025067
rosa_multiflora Rmu_sc0000240.1_g000021
rosa_roxburghii Rroxscaffold_6G00420080
rosa_rugosa Rorug03G0037400
rosa_samantha Rh3AG096000 Rh3BG098600 Rh3CG100600 Rh3DG100000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 569
Acc16I TGCGCA 1 cut(s) 602
AccI GTMKAC 1 cut(s) 573
AccII CGCG 1 cut(s) 366
AciI CCGC 6 cut(s) 67, 91, 97, 100, 336, 359
AclWI GGATC 1 cut(s) 417
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 1 cut(s) 455
AgsI TTSAA 1 cut(s) 427
AjnI CCWGG 1 cut(s) 501
AjuI GAANNNNNNNTTGG 2 cut(s) 482, 514
AluBI AGCT 1 cut(s) 493
AluI AGCT 1 cut(s) 493
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 2 cut(s) 83, 572
AlwI GGATC 1 cut(s) 417
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 3 cut(s) 3, 44, 244
ApeKI GCWGC 2 cut(s) 69, 338
AspLEI GCGC 1 cut(s) 603
AsuHPI GGTGA 1 cut(s) 532
AvaI CYCGRG 1 cut(s) 176
BauI CACGAG 2 cut(s) 73, 149
Bbv12I GWGCWC 1 cut(s) 31
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 56, 325
BceAI ACGGC 4 cut(s) 59, 148, 259, 460
BcgI CGANNNNNNTGC 2 cut(s) 311, 345
BciT130I CCWGG 1 cut(s) 503
BclI TGATCA 1 cut(s) 537
BcoDI GTCTC 2 cut(s) 83, 572
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 4 cut(s) 70, 100, 339, 360
BlsI GCNGC 4 cut(s) 71, 101, 340, 361
Bme1390I CCNGG 1 cut(s) 503
BmeT110I CYCGRG 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 503
BmsI GCATC 3 cut(s) 111, 359, 588
Bpu10I CCTNAGC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 53
BsaI GGTCTC 1 cut(s) 572
BsaXI ACNNNNNCTCC 6 cut(s) 13, 43, 251, 281, 374, 404
Bse1I ACTGG 1 cut(s) 28
Bse3DI GCAATG 1 cut(s) 517
BseBI CCWGG 1 cut(s) 503
BseGI GGATG 1 cut(s) 225
BseMI GCAATG 1 cut(s) 517
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 2 cut(s) 416, 613
BseXI GCAGC 2 cut(s) 56, 325
BsgI GTGCAG 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 366
BshFI GGCC 3 cut(s) 5, 46, 246
BsiHKAI GWGCWC 1 cut(s) 31
BsiHKCI CYCGRG 1 cut(s) 176
BslFI GGGAC 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 83, 572
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 3 cut(s) 5, 46, 246
Bso31I GGTCTC 1 cut(s) 572
BsoBI CYCGRG 1 cut(s) 176
Bsp1286I GDGCHC 1 cut(s) 31
Bsp143I GATC 3 cut(s) 153, 409, 537
BspACI CCGC 6 cut(s) 67, 91, 97, 100, 336, 359
BspANI GGCC 3 cut(s) 5, 46, 246
BspCNI CTCAG 1 cut(s) 334
BspFNI CGCG 1 cut(s) 366
BspHI TCATGA 1 cut(s) 559
BspPI GGATC 1 cut(s) 417
BspTNI GGTCTC 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 517
BsrI ACTGG 1 cut(s) 28
BssMI GATC 3 cut(s) 153, 409, 537
BssNI GRCGYC 1 cut(s) 53
BssSI CACGAG 2 cut(s) 73, 149
Bst2BI CACGAG 2 cut(s) 73, 149
Bst2UI CCWGG 1 cut(s) 503
Bst4CI ACNGT 4 cut(s) 13, 145, 481, 566
BstACI GRCGYC 1 cut(s) 53
BstAPI GCANNNNNTGC 1 cut(s) 598
BstC8I GCNNGC 1 cut(s) 364
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 225
BstFNI CGCG 1 cut(s) 366
BstHHI GCGC 1 cut(s) 603
BstKTI GATC 3 cut(s) 156, 412, 540
BstMAI GTCTC 2 cut(s) 83, 572
BstMBI GATC 3 cut(s) 153, 409, 537
BstMWI GCNNNNNNNGC 6 cut(s) 69, 99, 338, 347, 356, 598
BstNI CCWGG 1 cut(s) 503
BstSCI CCNGG 1 cut(s) 501
BstSFI CTRYAG 1 cut(s) 9
BstUI CGCG 1 cut(s) 366
BstV1I GCAGC 2 cut(s) 56, 325
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BsuRI GGCC 3 cut(s) 5, 46, 246
BtsCI GGATG 1 cut(s) 225
BtsIMutI CAGTG 2 cut(s) 141, 186
Cac8I GCNNGC 1 cut(s) 364
CaiI CAGNNNCTG 1 cut(s) 23
CciI TCATGA 1 cut(s) 559
CfoI GCGC 1 cut(s) 603
CseI GACGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 454
CviAII CATG 2 cut(s) 560, 595
CviJI RGCY 8 cut(s) 5, 46, 135, 159, 164, 246, 464, 493
CviKI_1 RGCY 8 cut(s) 5, 46, 135, 159, 164, 246, 464, 493
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 1 cut(s) 342
DpnI GATC 3 cut(s) 155, 411, 539
DpnII GATC 3 cut(s) 153, 409, 537
DrdI GACNNNNNNGTC 1 cut(s) 569
DseDI GACNNNNNNGTC 1 cut(s) 569
EciI GGCGGA 2 cut(s) 80, 86
Eco31I GGTCTC 1 cut(s) 572
Eco88I CYCGRG 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 501
FaeI CATG 2 cut(s) 563, 598
FaiI YATR 7 cut(s) 113, 288, 389, 458, 555, 561, 596
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 559, 594
FbaI TGATCA 1 cut(s) 537
FblI GTMKAC 1 cut(s) 573
Fnu4HI GCNGC 4 cut(s) 70, 100, 339, 360
FokI GGATG 1 cut(s) 212
Fsp4HI GCNGC 4 cut(s) 70, 100, 339, 360
FspAI RTGCGCAY 1 cut(s) 602
FspBI CTAG 1 cut(s) 167
FspI TGCGCA 1 cut(s) 602
GlaI GCGC 1 cut(s) 602
GluI GCNGC 4 cut(s) 70, 100, 339, 360
HaeIII GGCC 3 cut(s) 5, 46, 246
HgaI GACGC 1 cut(s) 42
HhaI GCGC 1 cut(s) 603
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 2 cut(s) 563, 598
Hin6I GCGC 1 cut(s) 601
HinP1I GCGC 1 cut(s) 601
HincII GTYRAC 2 cut(s) 550, 574
HindII GTYRAC 2 cut(s) 550, 574
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 3 cut(s) 76, 404, 498
HphI GGTGA 1 cut(s) 532
Hpy166II GTNNAC 3 cut(s) 148, 550, 574
Hpy188I TCNGA 4 cut(s) 199, 409, 418, 531
Hpy188III TCNNGA 1 cut(s) 560
Hpy8I GTNNAC 3 cut(s) 148, 550, 574
Hpy99I CGWCG 8 cut(s) 58, 245, 260, 320, 323, 371, 452, 575
HpyAV CCTTC 4 cut(s) 42, 57, 232, 500
HpyCH4III ACNGT 4 cut(s) 13, 145, 481, 566
HpyCH4V TGCA 2 cut(s) 72, 475
HpyF10VI GCNNNNNNNGC 6 cut(s) 69, 99, 338, 347, 356, 598
HpyF3I CTNAG 1 cut(s) 342
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 2 cut(s) 563, 598
HspAI GCGC 1 cut(s) 601
Ksp22I TGATCA 1 cut(s) 537
Kzo9I GATC 3 cut(s) 153, 409, 537
LmnI GCTCC 2 cut(s) 34, 347
LpnPI CCDG 4 cut(s) 3, 9, 488, 515
Lsp1109I GCAGC 2 cut(s) 56, 325
LweI GCATC 3 cut(s) 111, 359, 588
MaeI CTAG 1 cut(s) 167
MalI GATC 3 cut(s) 155, 411, 539
MboI GATC 3 cut(s) 153, 409, 537
MboII GAAGA 1 cut(s) 544
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 31
MlyI GAGTC 2 cut(s) 85, 413
MmeI TCCRAC 3 cut(s) 222, 253, 387
MseI TTAA 3 cut(s) 38, 588, 628
MslI CAYNNNNRTG 1 cut(s) 558
MspA1I CMGCKG 3 cut(s) 69, 338, 359
MspR9I CCNGG 1 cut(s) 503
MvaI CCWGG 1 cut(s) 503
MvnI CGCG 1 cut(s) 366
MwoI GCNNNNNNNGC 6 cut(s) 69, 99, 338, 347, 356, 598
NdeII GATC 3 cut(s) 153, 409, 537
NlaIII CATG 2 cut(s) 563, 598
NsbI TGCGCA 1 cut(s) 602
PagI TCATGA 1 cut(s) 559
PcsI WCGNNNNNNNCGW 2 cut(s) 570, 579
PfeI GAWTC 1 cut(s) 498
PfoI TCCNGGA 1 cut(s) 501
PkrI GCNGC 4 cut(s) 71, 101, 340, 361
PleI GAGTC 2 cut(s) 84, 412
PpsI GAGTC 2 cut(s) 84, 412
Psp6I CCWGG 1 cut(s) 501
PspGI CCWGG 1 cut(s) 501
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 409
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
RseI CAYNNNNRTG 1 cut(s) 558
SalI GTCGAC 1 cut(s) 572
SaqAI TTAA 3 cut(s) 38, 588, 628
SatI GCNGC 4 cut(s) 70, 100, 339, 360
Sau3AI GATC 3 cut(s) 153, 409, 537
SchI GAGTC 2 cut(s) 85, 413
ScrFI CCNGG 1 cut(s) 503
SduI GDGCHC 1 cut(s) 31
SetI ASST 6 cut(s) 204, 296, 327, 432, 495, 545
SfaNI GCATC 3 cut(s) 111, 359, 588
SfcI CTRYAG 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 558
SsiI CCGC 6 cut(s) 67, 91, 97, 100, 336, 359
SspMI CTAG 1 cut(s) 167
StyD4I CCNGG 1 cut(s) 501
TaaI ACNGT 4 cut(s) 13, 145, 481, 566
TaqI TCGA 4 cut(s) 117, 255, 573, 582
TauI GCSGC 2 cut(s) 102, 362
TfiI GAWTC 1 cut(s) 498
Tru1I TTAA 3 cut(s) 38, 588, 628
Tru9I TTAA 3 cut(s) 38, 588, 628
TscAI CASTG 2 cut(s) 148, 193
TseI GCWGC 2 cut(s) 69, 338
TspDTI ATGAA 2 cut(s) 127, 214
TspGWI ACGGA 3 cut(s) 384, 535, 598
TspRI CASTG 2 cut(s) 148, 193
XmiI GTMKAC 1 cut(s) 573
XspI CTAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.