Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0013009

Physical Location & Seq

Genomic Coordinates Reverse (-)

27032232 .. 27032861

Gene Structure 630 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1178900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 630 bp

ATGGCCTCTACAGTTTCAGGGACTAGTGCTCCGTCGTTAAAACGGCCTTCGGGGTCGTTTCGCCTCCGCTGCACGAGTCTCAACTCCCTCCGCCTCCGCCGCATCTTTGATATGTTCGACAAGAACAAAGACGGCTTCATCACTGTCCACGAGATCAGCCAAGCCCTAGCACTTCTCGGGTTAGACACTGAAATCTCCGACCTCCATTCCACGATAAAATCCTTCATCCAACCCTCAAACGACGGCCTCAACTTCGACGACTTCCTCTCCCTCCACCAATCCCTCTATGACACCTATTTCGACAATAATAACAACGACGAAGAGGTTGTTGCTTCCGCTGCTGAGGGAGCATCAACAGCGGCATGCGACGGAAACTCAAAGGAGAATATGTTGTCGCAAGAGGAGTCGGATCTTTCGGAGGCGTTCAAGGTGTTTGATGAGGACGGCGACGGGTACATATCGGCTAAAGAGTTGCAGACGGTGCTTGCCAAGCTTGGATTCCAGGAAGGCAATGAGATTGATAGAGTTCAGAAGATGATCACCTCCGTTGACCGCAACCATGACGGCCTCGTTGACTTGTTCGAGTTTAAGCACATGATGCGCACTACCGTACTTGTTCACTCCTGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010035 GO:0010038 GO:0010090 GO:0010091 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0042221 GO:0043167 GO:0043169 GO:0046686 GO:0046872 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0071840 GO:0090558 GO:0090626

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

210

Amino Acids

23.2

Weight (kDa)

4.66

Isoelectric Point (pI)

42.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	31 - 91	4.2e-08	EF-hand domain pair
EF-hand_6	PF13405	31 - 60	3.2e-08	EF-hand domain
EF-hand_1	PF00036	32 - 57	5.8e-07	EF hand domain
EF-hand_7	PF13499	135 - 201	3.8e-14	EF-hand domain pair
EF-hand_1	PF00036	138 - 165	5.3e-08	EF hand domain
EF-hand_6	PF13405	138 - 166	2.9e-08	EF-hand domain
EF-hand_5	PF13202	141 - 162	9.4e-07	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0013009)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20780 AT5G44460
fragaria_vesca	FvH4_6g09300
malus_domestica	MD04G1178900.v1.1 MD07G1157000.v1.1 MD12G1194200.v1.1
prunus_persica	Prupe.6G298900_v2.0.a1
pyrus_communis	pycom04g15820 pycom12g18040
rosa_chinensis	RchiOBHm_Chr3g0459681
rosa_laevigata	RLG00000025067
rosa_multiflora	Rmu_sc0000240.1_g000021
rosa_roxburghii	Rroxscaffold_6G00420080
rosa_rugosa	Rorug03G0037400
rosa_samantha	Rh3AG096000 Rh3BG098600 Rh3CG100600 Rh3DG100000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	602
AciI	CCGC	7 cut(s)	67, 91, 97, 100, 336, 359, 553
AclWI	GGATC	1 cut(s)	417
AfaI	GTAC	2 cut(s)	455, 612
AgsI	TTSAA	1 cut(s)	427
AhlI	ACTAGT	1 cut(s)	23
AjnI	CCWGG	1 cut(s)	501
AjuI	GAANNNNNNNTTGG	2 cut(s)	482, 514
AluBI	AGCT	1 cut(s)	493
AluI	AGCT	1 cut(s)	493
Alw21I	GWGCWC	1 cut(s)	31
Alw26I	GTCTC	1 cut(s)	83
AlwI	GGATC	1 cut(s)	417
Ama87I	CYCGRG	1 cut(s)	176
AoxI	GGCC	4 cut(s)	3, 44, 244, 565
ApeKI	GCWGC	2 cut(s)	69, 338
ArsI	GACNNNNNNTTYG	2 cut(s)	281, 313
AspLEI	GCGC	1 cut(s)	603
AsuHPI	GGTGA	1 cut(s)	532
AvaI	CYCGRG	1 cut(s)	176
BauI	CACGAG	2 cut(s)	73, 149
Bbv12I	GWGCWC	1 cut(s)	31
BbvCI	CCTCAGC	1 cut(s)	342
BbvI	GCAGC	2 cut(s)	56, 325
BceAI	ACGGC	5 cut(s)	59, 148, 259, 460, 580
BciT130I	CCWGG	1 cut(s)	503
BclI	TGATCA	1 cut(s)	537
BcoDI	GTCTC	1 cut(s)	83
BcuI	ACTAGT	1 cut(s)	23
BfaI	CTAG	2 cut(s)	24, 167
BfmI	CTRYAG	1 cut(s)	9
BisI	GCNGC	4 cut(s)	70, 100, 339, 360
BlsI	GCNGC	4 cut(s)	71, 101, 340, 361
Bme1390I	CCNGG	1 cut(s)	503
BmeT110I	CYCGRG	1 cut(s)	176
BmrFI	CCNGG	1 cut(s)	503
BmsI	GCATC	3 cut(s)	111, 359, 588
Bpu10I	CCTNAGC	1 cut(s)	342
BsaXI	ACNNNNNCTCC	6 cut(s)	13, 43, 251, 281, 374, 404
Bse3DI	GCAATG	1 cut(s)	517
BseBI	CCWGG	1 cut(s)	503
BseGI	GGATG	1 cut(s)	225
BseMI	GCAATG	1 cut(s)	517
BseMII	CTCAG	1 cut(s)	333
BseRI	GAGGAG	1 cut(s)	416
BseXI	GCAGC	2 cut(s)	56, 325
BsgI	GTGCAG	1 cut(s)	55
BshFI	GGCC	4 cut(s)	5, 46, 246, 567
BsiHKAI	GWGCWC	1 cut(s)	31
BsiHKCI	CYCGRG	1 cut(s)	176
BslFI	GGGAC	1 cut(s)	34
BsmAI	GTCTC	1 cut(s)	83
BsmFI	GGGAC	1 cut(s)	34
BsnI	GGCC	4 cut(s)	5, 46, 246, 567
BsoBI	CYCGRG	1 cut(s)	176
Bsp1286I	GDGCHC	1 cut(s)	31
Bsp143I	GATC	3 cut(s)	153, 409, 537
BspACI	CCGC	7 cut(s)	67, 91, 97, 100, 336, 359, 553
BspANI	GGCC	4 cut(s)	5, 46, 246, 567
BspCNI	CTCAG	1 cut(s)	334
BspPI	GGATC	1 cut(s)	417
BsrDI	GCAATG	1 cut(s)	517
BssMI	GATC	3 cut(s)	153, 409, 537
BssSI	CACGAG	2 cut(s)	73, 149
Bst2BI	CACGAG	2 cut(s)	73, 149
Bst2UI	CCWGG	1 cut(s)	503
Bst4CI	ACNGT	4 cut(s)	13, 145, 481, 610
Bst6I	CTCTTC	1 cut(s)	315
BstAPI	GCANNNNNTGC	2 cut(s)	481, 598
BstC8I	GCNNGC	2 cut(s)	364, 486
BstDEI	CTNAG	1 cut(s)	342
BstF5I	GGATG	1 cut(s)	225
BstHHI	GCGC	1 cut(s)	603
BstKTI	GATC	3 cut(s)	156, 412, 540
BstMAI	GTCTC	1 cut(s)	83
BstMBI	GATC	3 cut(s)	153, 409, 537
BstMWI	GCNNNNNNNGC	8 cut(s)	69, 99, 338, 347, 356, 481, 490, 598
BstNI	CCWGG	1 cut(s)	503
BstNSI	RCATGY	1 cut(s)	366
BstSCI	CCNGG	1 cut(s)	501
BstSFI	CTRYAG	1 cut(s)	9
BstV1I	GCAGC	2 cut(s)	56, 325
BstX2I	RGATCY	1 cut(s)	409
BstYI	RGATCY	1 cut(s)	409
BsuRI	GGCC	4 cut(s)	5, 46, 246, 567
BtsCI	GGATG	1 cut(s)	225
BtsIMutI	CAGTG	2 cut(s)	141, 186
Cac8I	GCNNGC	2 cut(s)	364, 486
CfoI	GCGC	1 cut(s)	603
Csp6I	GTAC	2 cut(s)	454, 611
CviAII	CATG	3 cut(s)	363, 560, 595
CviJI	RGCY	9 cut(s)	5, 46, 135, 159, 164, 246, 464, 493, 567
CviKI_1	RGCY	9 cut(s)	5, 46, 135, 159, 164, 246, 464, 493, 567
CviQI	GTAC	2 cut(s)	454, 611
DdeI	CTNAG	1 cut(s)	342
DpnI	GATC	3 cut(s)	155, 411, 539
DpnII	GATC	3 cut(s)	153, 409, 537
Eam1104I	CTCTTC	1 cut(s)	315
EarI	CTCTTC	1 cut(s)	315
EciI	GGCGGA	2 cut(s)	80, 86
Eco88I	CYCGRG	1 cut(s)	176
EcoRII	CCWGG	1 cut(s)	501
FaeI	CATG	3 cut(s)	366, 563, 598
FaiI	YATR	7 cut(s)	113, 288, 364, 389, 458, 561, 596
FaqI	GGGAC	1 cut(s)	34
FatI	CATG	3 cut(s)	362, 559, 594
FbaI	TGATCA	1 cut(s)	537
Fnu4HI	GCNGC	4 cut(s)	70, 100, 339, 360
FokI	GGATG	1 cut(s)	212
Fsp4HI	GCNGC	4 cut(s)	70, 100, 339, 360
FspAI	RTGCGCAY	1 cut(s)	602
FspBI	CTAG	2 cut(s)	24, 167
FspI	TGCGCA	1 cut(s)	602
GlaI	GCGC	1 cut(s)	602
GluI	GCNGC	4 cut(s)	70, 100, 339, 360
HaeIII	GGCC	4 cut(s)	5, 46, 246, 567
HhaI	GCGC	1 cut(s)	603
Hin1II	CATG	3 cut(s)	366, 563, 598
Hin6I	GCGC	1 cut(s)	601
HinP1I	GCGC	1 cut(s)	601
HincII	GTYRAC	2 cut(s)	550, 574
HindII	GTYRAC	2 cut(s)	550, 574
HindIII	AAGCTT	1 cut(s)	491
HinfI	GANTC	3 cut(s)	76, 404, 498
HphI	GGTGA	1 cut(s)	532
Hpy166II	GTNNAC	4 cut(s)	148, 550, 574, 619
Hpy188I	TCNGA	4 cut(s)	199, 409, 418, 531
Hpy8I	GTNNAC	4 cut(s)	148, 550, 574, 619
Hpy99I	CGWCG	6 cut(s)	37, 245, 260, 320, 371, 452
HpyAV	CCTTC	3 cut(s)	57, 232, 500
HpyCH4III	ACNGT	4 cut(s)	13, 145, 481, 610
HpyCH4V	TGCA	2 cut(s)	72, 475
HpyF10VI	GCNNNNNNNGC	8 cut(s)	69, 99, 338, 347, 356, 481, 490, 598
HpyF3I	CTNAG	1 cut(s)	342
Hsp92II	CATG	3 cut(s)	366, 563, 598
HspAI	GCGC	1 cut(s)	601
Ksp22I	TGATCA	1 cut(s)	537
Kzo9I	GATC	3 cut(s)	153, 409, 537
LmnI	GCTCC	2 cut(s)	34, 347
LpnPI	CCDG	3 cut(s)	3, 488, 515
Lsp1109I	GCAGC	2 cut(s)	56, 325
LweI	GCATC	3 cut(s)	111, 359, 588
MaeI	CTAG	2 cut(s)	24, 167
MalI	GATC	3 cut(s)	155, 411, 539
MboI	GATC	3 cut(s)	153, 409, 537
MboII	GAAGA	2 cut(s)	332, 544
MflI	RGATCY	1 cut(s)	409
MhlI	GDGCHC	1 cut(s)	31
MlyI	GAGTC	2 cut(s)	85, 413
MmeI	TCCRAC	3 cut(s)	222, 253, 387
MseI	TTAA	3 cut(s)	38, 588, 628
MspA1I	CMGCKG	3 cut(s)	69, 338, 359
MspR9I	CCNGG	1 cut(s)	503
MvaI	CCWGG	1 cut(s)	503
MwoI	GCNNNNNNNGC	8 cut(s)	69, 99, 338, 347, 356, 481, 490, 598
NdeII	GATC	3 cut(s)	153, 409, 537
NlaIII	CATG	3 cut(s)	366, 563, 598
NsbI	TGCGCA	1 cut(s)	602
NspI	RCATGY	1 cut(s)	366
PaeI	GCATGC	1 cut(s)	366
PfeI	GAWTC	1 cut(s)	498
PfoI	TCCNGGA	1 cut(s)	501
PkrI	GCNGC	4 cut(s)	71, 101, 340, 361
PleI	GAGTC	2 cut(s)	84, 412
PpsI	GAGTC	2 cut(s)	84, 412
Psp6I	CCWGG	1 cut(s)	501
PspGI	CCWGG	1 cut(s)	501
PsuI	RGATCY	1 cut(s)	409
RsaI	GTAC	2 cut(s)	455, 612
RsaNI	GTAC	2 cut(s)	454, 611
SaqAI	TTAA	3 cut(s)	38, 588, 628
SatI	GCNGC	4 cut(s)	70, 100, 339, 360
Sau3AI	GATC	3 cut(s)	153, 409, 537
SchI	GAGTC	2 cut(s)	85, 413
ScrFI	CCNGG	1 cut(s)	503
SduI	GDGCHC	1 cut(s)	31
SetI	ASST	6 cut(s)	204, 296, 327, 432, 495, 545
SfaNI	GCATC	3 cut(s)	111, 359, 588
SfcI	CTRYAG	1 cut(s)	9
SpeI	ACTAGT	1 cut(s)	23
SphI	GCATGC	1 cut(s)	366
SsiI	CCGC	7 cut(s)	67, 91, 97, 100, 336, 359, 553
SspMI	CTAG	2 cut(s)	24, 167
StyD4I	CCNGG	1 cut(s)	501
TaaI	ACNGT	4 cut(s)	13, 145, 481, 610
TaqI	TCGA	4 cut(s)	117, 255, 300, 582
TauI	GCSGC	2 cut(s)	102, 362
TfiI	GAWTC	1 cut(s)	498
Tru1I	TTAA	3 cut(s)	38, 588, 628
Tru9I	TTAA	3 cut(s)	38, 588, 628
TscAI	CASTG	2 cut(s)	148, 193
TseI	GCWGC	2 cut(s)	69, 338
TspDTI	ATGAA	2 cut(s)	127, 214
TspGWI	ACGGA	3 cut(s)	21, 384, 535
TspRI	CASTG	2 cut(s)	148, 193
XceI	RCATGY	1 cut(s)	366
XspI	CTAG	2 cut(s)	24, 167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.