FvH4_6g09300

Calcium-binding protein CML42-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013009
Physical Location & Seq
Forward (+)
5516781 .. 5518331
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g09300.t1

Sequence Viewer

Length: 657 bp
ATGGAAGCAGCAGCAGCAGCGCCTTCTTCAGCAGGAACGTCAAGTGAACGCACCCTCGCCAAGAGGCCCTCCGCCTCTTCCTCCTTTCGTCTCCGCTGCCCGAGTCTCAACTCTCTCCGCCTCCTCCGCATCTTTGACATTTTCGACAAGAACCGCGATGGCATGGTCAGCGTCGACGAGATCAACCAAGCCCTAAGACTCCTCGGCCTCGACGTCGAAGTCTCCGACCTCGAGGCCACCATAAAGACCTTCATCCAGCCCGGAAACGAAGGTCTTACGTACGATGACTTTGTAGGGCTACACCAGTCGCTTTATGATACGTTCTTCTTCGACGGTGATGACATTGAAAGCGCGGTGGAAGAGGCAGCGGCGTTGGTGGTGGCTGCGAGGGACGAGGAGGAAGCGAAGAAGTTGTTGCAGGAGGAGTCGGATCTAAGGGAGGCGTTCAAGGTGTTTGATGAAGACGGAGACGGGTTCATATCGGCAAAGGAGCTGCAGACGGTGCTTGGGAAGCTAGGGTTCCCGGAAGGGAATGAGATTGATAGAGTGGAGAGGATGATTACTTCCGTTGATCGGAACCACGACGGCCTTGTTGATTTGTTCGAGTTTAAGGACATGATGCGCAGTACCGTTCTTGTTCCTTCCTCTTGTTCCTAA

Protein Analysis

219

Amino Acids

24.08

Weight (kDa)

4.4

Isoelectric Point (pI)

48.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 40 - 99 8.1e-08 EF-hand domain pair
EF-hand_6 PF13405 41 - 69 6.4e-07 EF-hand domain
EF-hand_7 PF13499 142 - 208 2.2e-13 EF-hand domain pair
EF-hand_6 PF13405 144 - 173 1.3e-08 EF-hand domain
EF-hand_1 PF00036 144 - 171 6.4e-08 EF hand domain
EF-hand_5 PF13202 145 - 169 2.4e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20780 AT5G44460
fragaria_vesca FvH4_6g09300
malus_domestica MD04G1178900.v1.1 MD07G1157000.v1.1 MD12G1194200.v1.1
prunus_persica Prupe.6G298900_v2.0.a1
pyrus_communis pycom04g15820 pycom12g18040
rosa_chinensis RchiOBHm_Chr3g0459681
rosa_laevigata RLG00000025067
rosa_multiflora Rmu_sc0000240.1_g000021
rosa_roxburghii Rroxscaffold_6G00420080
rosa_rugosa Rorug03G0037400
rosa_samantha Rh3AG096000 Rh3BG098600 Rh3CG100600 Rh3DG100000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AatII GACGTC 1 cut(s) 216
AbsI CCTCGAGG 1 cut(s) 230
Acc16I TGCGCA 1 cut(s) 623
AccI GTMKAC 1 cut(s) 174
AccII CGCG 2 cut(s) 156, 353
AciI CCGC 7 cut(s) 72, 94, 118, 127, 154, 353, 368
AclWI GGATC 1 cut(s) 438
AcuI CTGAAG 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 213
AfaI GTAC 2 cut(s) 281, 628
AfiI CCNNNNNNNGG 1 cut(s) 573
AgsI TTSAA 2 cut(s) 347, 448
AluBI AGCT 2 cut(s) 493, 514
AluI AGCT 2 cut(s) 493, 514
Alw26I GTCTC 4 cut(s) 95, 110, 226, 462
AlwI GGATC 1 cut(s) 438
Ama87I CYCGRG 2 cut(s) 100, 230
AoxI GGCC 4 cut(s) 65, 205, 234, 586
ApeKI GCWGC 8 cut(s) 8, 11, 14, 17, 96, 365, 383, 493
AspLEI GCGC 3 cut(s) 22, 353, 624
AspS9I GGNCC 1 cut(s) 66
AsuC2I CCSGG 2 cut(s) 261, 524
AsuHPI GGTGA 1 cut(s) 347
AvaI CYCGRG 2 cut(s) 100, 230
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 8 cut(s) 20, 23, 26, 29, 83, 370, 377, 480
BccI CCATC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 601
BcnI CCSGG 2 cut(s) 261, 524
BcoDI GTCTC 4 cut(s) 95, 110, 226, 462
BfaI CTAG 1 cut(s) 515
BfmI CTRYAG 1 cut(s) 494
BfoI RGCGCY 1 cut(s) 23
BisI GCNGC 9 cut(s) 9, 12, 15, 18, 97, 366, 369, 384, 494
BlsI GCNGC 9 cut(s) 10, 13, 16, 19, 98, 367, 370, 385, 495
Bme1390I CCNGG 2 cut(s) 261, 524
BmeT110I CYCGRG 2 cut(s) 100, 230
BmgT120I GGNCC 1 cut(s) 66
BmiI GGNNCC 2 cut(s) 521, 578
BmrFI CCNGG 2 cut(s) 261, 524
BmsI GCATC 2 cut(s) 138, 609
BpiI GAAGAC 1 cut(s) 468
BpuMI CCSGG 2 cut(s) 261, 524
BsaAI YACGTR 1 cut(s) 279
BsaHI GRCGYC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 202
Bsc4I CCNNNNNNNGG 1 cut(s) 573
Bse1I ACTGG 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 202
BseGI GGATG 2 cut(s) 252, 561
BseLI CCNNNNNNNGG 1 cut(s) 573
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 4 cut(s) 113, 191, 410, 437
BseXI GCAGC 8 cut(s) 20, 23, 26, 29, 83, 370, 377, 480
Bsh1236I CGCG 2 cut(s) 156, 353
BshFI GGCC 4 cut(s) 67, 207, 236, 588
BsiHKCI CYCGRG 2 cut(s) 100, 230
BsiSI CCGG 2 cut(s) 261, 524
BsiWI CGTACG 1 cut(s) 279
BslFI GGGAC 1 cut(s) 404
BslI CCNNNNNNNGG 1 cut(s) 573
BsmAI GTCTC 4 cut(s) 95, 110, 226, 462
BsmBI CGTCTC 2 cut(s) 95, 462
BsmFI GGGAC 1 cut(s) 404
BsnI GGCC 4 cut(s) 67, 207, 236, 588
BsoBI CYCGRG 2 cut(s) 100, 230
Bsp143I GATC 3 cut(s) 180, 430, 571
BspACI CCGC 7 cut(s) 72, 94, 118, 127, 154, 353, 368
BspANI GGCC 4 cut(s) 67, 207, 236, 588
BspFNI CGCG 2 cut(s) 156, 353
BspLI GGNNCC 2 cut(s) 521, 578
BspMAI CTGCAG 1 cut(s) 498
BspPI GGATC 1 cut(s) 438
BsrI ACTGG 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 202
BssMI GATC 3 cut(s) 180, 430, 571
BssNI GRCGYC 1 cut(s) 213
Bst4CI ACNGT 3 cut(s) 335, 502, 631
Bst6I CTCTTC 2 cut(s) 82, 354
BstACI GRCGYC 1 cut(s) 213
BstAPI GCANNNNNTGC 1 cut(s) 502
BstBAI YACGTR 1 cut(s) 279
BstDEI CTNAG 2 cut(s) 194, 434
BstF5I GGATG 2 cut(s) 252, 561
BstFNI CGCG 2 cut(s) 156, 353
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 3 cut(s) 22, 353, 624
BstKTI GATC 3 cut(s) 183, 433, 574
BstMAI GTCTC 4 cut(s) 95, 110, 226, 462
BstMBI GATC 3 cut(s) 180, 430, 571
BstMWI GCNNNNNNNGC 6 cut(s) 14, 17, 126, 168, 502, 511
BstSCI CCNGG 2 cut(s) 259, 522
BstSFI CTRYAG 1 cut(s) 494
BstSNI TACGTA 1 cut(s) 279
BstUI CGCG 2 cut(s) 156, 353
BstV1I GCAGC 8 cut(s) 20, 23, 26, 29, 83, 370, 377, 480
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 1 cut(s) 430
BstYI RGATCY 1 cut(s) 430
BsuRI GGCC 4 cut(s) 67, 207, 236, 588
BtgZI GCGATG 1 cut(s) 171
BtsCI GGATG 2 cut(s) 252, 561
CfoI GCGC 3 cut(s) 22, 353, 624
Cfr13I GGNCC 1 cut(s) 66
CseI GACGC 1 cut(s) 160
Csp6I GTAC 2 cut(s) 280, 627
CviAII CATG 2 cut(s) 163, 616
CviQI GTAC 2 cut(s) 280, 627
DdeI CTNAG 2 cut(s) 194, 434
DpnI GATC 3 cut(s) 182, 432, 573
DpnII GATC 3 cut(s) 180, 430, 571
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Eam1104I CTCTTC 2 cut(s) 82, 354
EarI CTCTTC 2 cut(s) 82, 354
EciI GGCGGA 2 cut(s) 61, 107
Eco105I TACGTA 1 cut(s) 279
Eco57I CTGAAG 1 cut(s) 12
Eco88I CYCGRG 2 cut(s) 100, 230
EcoO109I RGGNCCY 1 cut(s) 66
Esp3I CGTCTC 2 cut(s) 95, 462
FaeI CATG 2 cut(s) 166, 619
FaiI YATR 5 cut(s) 164, 242, 315, 479, 617
FaqI GGGAC 1 cut(s) 404
FatI CATG 2 cut(s) 162, 615
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 9 cut(s) 9, 12, 15, 18, 97, 366, 369, 384, 494
FokI GGATG 2 cut(s) 239, 568
Fsp4HI GCNGC 9 cut(s) 9, 12, 15, 18, 97, 366, 369, 384, 494
FspBI CTAG 1 cut(s) 515
FspI TGCGCA 1 cut(s) 623
GlaI GCGC 3 cut(s) 21, 352, 623
GluI GCNGC 9 cut(s) 9, 12, 15, 18, 97, 366, 369, 384, 494
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 4 cut(s) 67, 207, 236, 588
HapII CCGG 2 cut(s) 261, 524
HgaI GACGC 1 cut(s) 160
HhaI GCGC 3 cut(s) 22, 353, 624
Hin1I GRCGYC 1 cut(s) 213
Hin1II CATG 2 cut(s) 166, 619
Hin6I GCGC 3 cut(s) 20, 351, 622
HinP1I GCGC 3 cut(s) 20, 351, 622
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 3 cut(s) 103, 198, 425
HpaII CCGG 2 cut(s) 261, 524
HphI GGTGA 1 cut(s) 347
Hpy166II GTNNAC 2 cut(s) 47, 175
Hpy188I TCNGA 3 cut(s) 226, 430, 576
Hpy8I GTNNAC 2 cut(s) 47, 175
Hpy99I CGWCG 6 cut(s) 176, 179, 215, 218, 335, 587
HpyAV CCTTC 5 cut(s) 33, 259, 263, 521, 651
HpyCH4III ACNGT 3 cut(s) 335, 502, 631
HpyCH4IV ACGT 4 cut(s) 38, 213, 278, 320
HpyCH4V TGCA 2 cut(s) 418, 496
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 17, 126, 168, 502, 511
HpyF3I CTNAG 2 cut(s) 194, 434
HpySE526I ACGT 4 cut(s) 38, 213, 278, 320
Hsp92I GRCGYC 1 cut(s) 213
Hsp92II CATG 2 cut(s) 166, 619
HspAI GCGC 3 cut(s) 20, 351, 622
Kzo9I GATC 3 cut(s) 180, 430, 571
LmnI GCTCC 1 cut(s) 490
LpnPI CCDG 6 cut(s) 18, 269, 274, 317, 404, 537
Lsp1109I GCAGC 8 cut(s) 20, 23, 26, 29, 83, 370, 377, 480
LweI GCATC 2 cut(s) 138, 609
MaeI CTAG 1 cut(s) 515
MaeII ACGT 4 cut(s) 38, 213, 278, 320
MalI GATC 3 cut(s) 182, 432, 573
MboI GATC 3 cut(s) 180, 430, 571
MboII GAAGA 7 cut(s) 18, 69, 316, 319, 371, 418, 473
MflI RGATCY 1 cut(s) 430
MlyI GAGTC 3 cut(s) 112, 192, 434
MmeI TCCRAC 2 cut(s) 249, 408
MseI TTAA 1 cut(s) 609
MspA1I CMGCKG 2 cut(s) 96, 368
MspI CCGG 2 cut(s) 261, 524
MspR9I CCNGG 2 cut(s) 261, 524
MvnI CGCG 2 cut(s) 156, 353
MwoI GCNNNNNNNGC 6 cut(s) 14, 17, 126, 168, 502, 511
NciI CCSGG 2 cut(s) 261, 524
NdeII GATC 3 cut(s) 180, 430, 571
NlaIII CATG 2 cut(s) 166, 619
NlaIV GGNNCC 2 cut(s) 521, 578
NmeAIII GCCGAG 1 cut(s) 183
NsbI TGCGCA 1 cut(s) 623
PaeR7I CTCGAG 1 cut(s) 230
PcsI WCGNNNNNNNCGW 2 cut(s) 210, 222
Pfl23II CGTACG 1 cut(s) 279
PfoI TCCNGGA 1 cut(s) 522
PkrI GCNGC 9 cut(s) 10, 13, 16, 19, 98, 367, 370, 385, 495
PleI GAGTC 3 cut(s) 111, 192, 433
PpsI GAGTC 3 cut(s) 111, 192, 433
Ppu21I YACGTR 1 cut(s) 279
PspLI CGTACG 1 cut(s) 279
PspN4I GGNNCC 2 cut(s) 521, 578
PspPI GGNCC 1 cut(s) 66
PspXI VCTCGAGB 1 cut(s) 230
PstI CTGCAG 1 cut(s) 498
PsuI RGATCY 1 cut(s) 430
RsaI GTAC 2 cut(s) 281, 628
RsaNI GTAC 2 cut(s) 280, 627
SalI GTCGAC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 609
SatI GCNGC 9 cut(s) 9, 12, 15, 18, 97, 366, 369, 384, 494
Sau3AI GATC 3 cut(s) 180, 430, 571
Sau96I GGNCC 1 cut(s) 66
SchI GAGTC 3 cut(s) 112, 192, 434
ScrFI CCNGG 2 cut(s) 261, 524
SfaNI GCATC 2 cut(s) 138, 609
SfcI CTRYAG 1 cut(s) 494
Sfr274I CTCGAG 1 cut(s) 230
SgrDI CGTCGACG 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 230
SmlI CTYRAG 1 cut(s) 230
SmoI CTYRAG 1 cut(s) 230
SnaBI TACGTA 1 cut(s) 279
SsiI CCGC 7 cut(s) 72, 94, 118, 127, 154, 353, 368
SspMI CTAG 1 cut(s) 515
StyD4I CCNGG 2 cut(s) 259, 522
TaaI ACNGT 3 cut(s) 335, 502, 631
TaiI ACGT 4 cut(s) 41, 216, 281, 323
TaqI TCGA 7 cut(s) 144, 174, 210, 216, 231, 330, 603
TauI GCSGC 1 cut(s) 371
Tru1I TTAA 1 cut(s) 609
Tru9I TTAA 1 cut(s) 609
TseI GCWGC 8 cut(s) 8, 11, 14, 17, 96, 365, 383, 493
TspDTI ATGAA 3 cut(s) 241, 466, 474
TspGWI ACGGA 2 cut(s) 480, 556
XhoI CTCGAG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 174
XspI CTAG 1 cut(s) 515
ZraI GACGTC 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.