Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0013009

Physical Location & Seq

Genomic Coordinates Reverse (-)

27576231 .. 27576848

Gene Structure 618 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1194200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 618 bp

ATGGCCTCTACAGTTTCAGGGACTGGCGTTCCTTCGTTAAAACGGCCTTCGCGGTCGTTTCGCCTCCGCTGCTCAAGTCTCAACTCTCTCCGGCTCCGCCGCATCTTCGATATGTACGACAAGAACAAGGACGGCTTCATCAGCGTCCACGAGATTAGCCAAGCCCTAGCCCTTCTCGGGTTGGACACTGAAATCTCCGACCTCCGTTCCACGATAAAATCCTTCATCCAACCCTCAAACGACGGCCTCAACTTCGACGACTTCGTCTCTCTCCACCAATCCCTCTATGACACCTATTTGGACAATAACAACGGCGATGAAGAAGCTGTCGCTGCCGCTGCTGAGGCAGCATCACCAGCTGCATGCGACAAGGAGAGTATGTTGTCGCAGGAGGAGTCTGATCTTTCGGAGGCGTTCAAGGTGTTTGATGAGGACGGCGACGGGTACATATCGGCTAAAGAGTTGCAGGTGGTGCTTGCCAAGCTCGGATTCCAGGAAGGCAACGAGATCGATAGAGTTGAGAAGATGATTACCTCCGTTGACCAGAACCACGACGGCCTCGTTGACTTGTTCGAGTTTAAGAACATGATGCGCACTGTTCTTGTTCCCAGCTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0009653 GO:0009888 GO:0009987 GO:0010026 GO:0010035 GO:0010038 GO:0010090 GO:0010091 GO:0016043 GO:0030154 GO:0032502 GO:0032989 GO:0042221 GO:0043167 GO:0043169 GO:0046686 GO:0046872 GO:0048468 GO:0048856 GO:0048869 GO:0050896 GO:0071840 GO:0090558 GO:0090626

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

206

Amino Acids

22.68

Weight (kDa)

4.52

Isoelectric Point (pI)

54.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_7	PF13499	31 - 90	4.5e-09	EF-hand domain pair
EF-hand_6	PF13405	31 - 60	5e-08	EF-hand domain
EF-hand_1	PF00036	32 - 57	9.2e-07	EF hand domain
EF-hand_7	PF13499	132 - 198	1.1e-14	EF-hand domain pair
EF-hand_1	PF00036	135 - 162	1.1e-07	EF hand domain
EF-hand_6	PF13405	135 - 163	7.3e-08	EF-hand domain
EF-hand_5	PF13202	138 - 158	3.9e-06	EF hand

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0013009)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G20780 AT5G44460
fragaria_vesca	FvH4_6g09300
malus_domestica	MD04G1178900.v1.1 MD07G1157000.v1.1 MD12G1194200.v1.1
prunus_persica	Prupe.6G298900_v2.0.a1
pyrus_communis	pycom04g15820 pycom12g18040
rosa_chinensis	RchiOBHm_Chr3g0459681
rosa_laevigata	RLG00000025067
rosa_multiflora	Rmu_sc0000240.1_g000021
rosa_roxburghii	Rroxscaffold_6G00420080
rosa_rugosa	Rorug03G0037400
rosa_samantha	Rh3AG096000 Rh3BG098600 Rh3CG100600 Rh3DG100000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	457
AasI	GACNNNNNNGTC	1 cut(s)	263
Acc16I	TGCGCA	1 cut(s)	593
Acc36I	ACCTGC	1 cut(s)	457
AccII	CGCG	1 cut(s)	52
AciI	CCGC	5 cut(s)	52, 67, 97, 100, 336
AfaI	GTAC	2 cut(s)	116, 446
AfiI	CCNNNNNNNGG	1 cut(s)	177
AgsI	TTSAA	1 cut(s)	418
AjnI	CCWGG	1 cut(s)	492
AjuI	GAANNNNNNNTTGG	2 cut(s)	473, 505
AluBI	AGCT	4 cut(s)	326, 359, 484, 612
AluI	AGCT	4 cut(s)	326, 359, 484, 612
Alw26I	GTCTC	2 cut(s)	83, 271
AlwNI	CAGNNNCTG	1 cut(s)	23
Ama87I	CYCGRG	1 cut(s)	176
AoxI	GGCC	4 cut(s)	3, 44, 244, 556
ApeKI	GCWGC	5 cut(s)	69, 332, 338, 347, 359
AspLEI	GCGC	1 cut(s)	594
AsuHPI	GGTGA	1 cut(s)	345
AvaI	CYCGRG	1 cut(s)	176
BauI	CACGAG	1 cut(s)	149
BbvCI	CCTCAGC	1 cut(s)	342
BbvI	GCAGC	5 cut(s)	56, 319, 325, 346, 359
BceAI	ACGGC	6 cut(s)	59, 148, 259, 328, 451, 571
BcgI	CGANNNNNNTGC	2 cut(s)	490, 524
BciT130I	CCWGG	1 cut(s)	494
BcoDI	GTCTC	2 cut(s)	83, 271
BfaI	CTAG	1 cut(s)	167
BfmI	CTRYAG	1 cut(s)	9
BfuAI	ACCTGC	1 cut(s)	457
BisI	GCNGC	7 cut(s)	70, 100, 333, 336, 339, 348, 360
BlsI	GCNGC	7 cut(s)	71, 101, 334, 337, 340, 349, 361
Bme1390I	CCNGG	1 cut(s)	494
BmeT110I	CYCGRG	1 cut(s)	176
BmiI	GGNNCC	1 cut(s)	95
BmrFI	CCNGG	1 cut(s)	494
BmsI	GCATC	3 cut(s)	111, 359, 579
Bpu10I	CCTNAGC	1 cut(s)	342
BpuEI	CTTGAG	1 cut(s)	58
Bsa29I	ATCGAT	1 cut(s)	510
BsaXI	ACNNNNNCTCC	2 cut(s)	365, 395
Bsc4I	CCNNNNNNNGG	1 cut(s)	177
Bse1I	ACTGG	1 cut(s)	28
BseBI	CCWGG	1 cut(s)	494
BseCI	ATCGAT	1 cut(s)	510
BseGI	GGATG	1 cut(s)	225
BseLI	CCNNNNNNNGG	1 cut(s)	177
BseMII	CTCAG	1 cut(s)	333
BseNI	ACTGG	1 cut(s)	28
BseRI	GAGGAG	1 cut(s)	407
BseXI	GCAGC	5 cut(s)	56, 319, 325, 346, 359
BseYI	CCCAGC	1 cut(s)	608
Bsh1236I	CGCG	1 cut(s)	52
Bsh1285I	CGRYCG	1 cut(s)	56
BshFI	GGCC	4 cut(s)	5, 46, 246, 558
BshVI	ATCGAT	1 cut(s)	510
BsiEI	CGRYCG	1 cut(s)	56
BsiHKCI	CYCGRG	1 cut(s)	176
BsiSI	CCGG	1 cut(s)	91
BslFI	GGGAC	1 cut(s)	34
BslI	CCNNNNNNNGG	1 cut(s)	177
BsmAI	GTCTC	2 cut(s)	83, 271
BsmBI	CGTCTC	1 cut(s)	271
BsmFI	GGGAC	1 cut(s)	34
BsnI	GGCC	4 cut(s)	5, 46, 246, 558
BsoBI	CYCGRG	1 cut(s)	176
Bsp143I	GATC	2 cut(s)	400, 507
BspACI	CCGC	5 cut(s)	52, 67, 97, 100, 336
BspANI	GGCC	4 cut(s)	5, 46, 246, 558
BspCNI	CTCAG	1 cut(s)	334
BspDI	ATCGAT	1 cut(s)	510
BspFNI	CGCG	1 cut(s)	52
BspLI	GGNNCC	1 cut(s)	95
BspMI	ACCTGC	1 cut(s)	457
BsrI	ACTGG	1 cut(s)	28
BssMI	GATC	2 cut(s)	400, 507
BssSI	CACGAG	1 cut(s)	149
Bst2BI	CACGAG	1 cut(s)	149
Bst2UI	CCWGG	1 cut(s)	494
Bst4CI	ACNGT	2 cut(s)	13, 598
BstAPI	GCANNNNNTGC	1 cut(s)	472
BstC8I	GCNNGC	2 cut(s)	364, 477
BstDEI	CTNAG	1 cut(s)	342
BstF5I	GGATG	1 cut(s)	225
BstFNI	CGCG	1 cut(s)	52
BstHHI	GCGC	1 cut(s)	594
BstKTI	GATC	2 cut(s)	403, 510
BstMAI	GTCTC	2 cut(s)	83, 271
BstMBI	GATC	2 cut(s)	400, 507
BstMCI	CGRYCG	1 cut(s)	56
BstMWI	GCNNNNNNNGC	9 cut(s)	69, 141, 332, 338, 344, 347, 356, 472, 481
BstNI	CCWGG	1 cut(s)	494
BstNSI	RCATGY	1 cut(s)	366
BstSCI	CCNGG	1 cut(s)	492
BstSFI	CTRYAG	1 cut(s)	9
BstUI	CGCG	1 cut(s)	52
BstV1I	GCAGC	5 cut(s)	56, 319, 325, 346, 359
Bsu15I	ATCGAT	1 cut(s)	510
BsuRI	GGCC	4 cut(s)	5, 46, 246, 558
BsuTUI	ATCGAT	1 cut(s)	510
BtgZI	GCGATG	1 cut(s)	330
BtsCI	GGATG	1 cut(s)	225
BtsIMutI	CAGTG	2 cut(s)	186, 594
BveI	ACCTGC	1 cut(s)	457
Cac8I	GCNNGC	2 cut(s)	364, 477
CaiI	CAGNNNCTG	1 cut(s)	23
CfoI	GCGC	1 cut(s)	594
ClaI	ATCGAT	1 cut(s)	510
CseI	GACGC	1 cut(s)	133
Csp6I	GTAC	2 cut(s)	115, 445
CviAII	CATG	2 cut(s)	363, 586
CviQI	GTAC	2 cut(s)	115, 445
DdeI	CTNAG	1 cut(s)	342
DpnI	GATC	2 cut(s)	402, 509
DpnII	GATC	2 cut(s)	400, 507
DrdI	GACNNNNNNGTC	1 cut(s)	263
DseDI	GACNNNNNNGTC	1 cut(s)	263
EciI	GGCGGA	1 cut(s)	86
Eco88I	CYCGRG	1 cut(s)	176
EcoRII	CCWGG	1 cut(s)	492
Esp3I	CGTCTC	1 cut(s)	271
FaeI	CATG	2 cut(s)	366, 589
FaiI	YATR	6 cut(s)	113, 288, 364, 380, 449, 587
FalI	AAGNNNNNCTT	2 cut(s)	119, 151
FaqI	GGGAC	1 cut(s)	34
FatI	CATG	2 cut(s)	362, 585
Fnu4HI	GCNGC	7 cut(s)	70, 100, 333, 336, 339, 348, 360
FokI	GGATG	1 cut(s)	212
Fsp4HI	GCNGC	7 cut(s)	70, 100, 333, 336, 339, 348, 360
FspAI	RTGCGCAY	1 cut(s)	593
FspBI	CTAG	1 cut(s)	167
FspI	TGCGCA	1 cut(s)	593
GlaI	GCGC	1 cut(s)	593
GluI	GCNGC	7 cut(s)	70, 100, 333, 336, 339, 348, 360
GsaI	CCCAGC	1 cut(s)	612
HaeIII	GGCC	4 cut(s)	5, 46, 246, 558
HapII	CCGG	1 cut(s)	91
HgaI	GACGC	1 cut(s)	133
HhaI	GCGC	1 cut(s)	594
Hin1II	CATG	2 cut(s)	366, 589
Hin6I	GCGC	1 cut(s)	592
HinP1I	GCGC	1 cut(s)	592
HincII	GTYRAC	2 cut(s)	541, 565
HindII	GTYRAC	2 cut(s)	541, 565
HinfI	GANTC	2 cut(s)	395, 489
HpaII	CCGG	1 cut(s)	91
HphI	GGTGA	1 cut(s)	345
Hpy166II	GTNNAC	3 cut(s)	148, 541, 565
Hpy188I	TCNGA	4 cut(s)	199, 400, 409, 488
Hpy188III	TCNNGA	1 cut(s)	615
Hpy8I	GTNNAC	3 cut(s)	148, 541, 565
Hpy99I	CGWCG	4 cut(s)	245, 260, 443, 557
HpyAV	CCTTC	5 cut(s)	42, 57, 182, 232, 491
HpyCH4III	ACNGT	2 cut(s)	13, 598
HpyCH4V	TGCA	2 cut(s)	362, 466
HpyF10VI	GCNNNNNNNGC	9 cut(s)	69, 141, 332, 338, 344, 347, 356, 472, 481
HpyF3I	CTNAG	1 cut(s)	342
Hsp92II	CATG	2 cut(s)	366, 589
HspAI	GCGC	1 cut(s)	592
Kzo9I	GATC	2 cut(s)	400, 507
LmnI	GCTCC	1 cut(s)	99
LpnPI	CCDG	9 cut(s)	3, 9, 104, 369, 374, 452, 479, 506, 557
Lsp1109I	GCAGC	5 cut(s)	56, 319, 325, 346, 359
LweI	GCATC	3 cut(s)	111, 359, 579
MaeI	CTAG	1 cut(s)	167
MalI	GATC	2 cut(s)	402, 509
MboI	GATC	2 cut(s)	400, 507
MboII	GAAGA	3 cut(s)	97, 332, 535
MlyI	GAGTC	1 cut(s)	404
MmeI	TCCRAC	3 cut(s)	162, 222, 253
MseI	TTAA	2 cut(s)	38, 579
MspA1I	CMGCKG	3 cut(s)	69, 338, 359
MspI	CCGG	1 cut(s)	91
MspR9I	CCNGG	1 cut(s)	494
MvaI	CCWGG	1 cut(s)	494
MvnI	CGCG	1 cut(s)	52
MwoI	GCNNNNNNNGC	9 cut(s)	69, 141, 332, 338, 344, 347, 356, 472, 481
NdeII	GATC	2 cut(s)	400, 507
NlaIII	CATG	2 cut(s)	366, 589
NlaIV	GGNNCC	1 cut(s)	95
NsbI	TGCGCA	1 cut(s)	593
NspI	RCATGY	1 cut(s)	366
PaeI	GCATGC	1 cut(s)	366
PaqCI	CACCTGC	1 cut(s)	457
PcsI	WCGNNNNNNNCGW	3 cut(s)	114, 261, 558
PfeI	GAWTC	1 cut(s)	489
PflFI	GACNNNGTC	1 cut(s)	263
PfoI	TCCNGGA	1 cut(s)	492
PkrI	GCNGC	7 cut(s)	71, 101, 334, 337, 340, 349, 361
PleI	GAGTC	1 cut(s)	403
PpsI	GAGTC	1 cut(s)	403
Psp6I	CCWGG	1 cut(s)	492
PspFI	CCCAGC	1 cut(s)	608
PspGI	CCWGG	1 cut(s)	492
PspN4I	GGNNCC	1 cut(s)	95
PstNI	CAGNNNCTG	1 cut(s)	23
PsyI	GACNNNGTC	1 cut(s)	263
PvuII	CAGCTG	1 cut(s)	359
RsaI	GTAC	2 cut(s)	116, 446
RsaNI	GTAC	2 cut(s)	115, 445
SaqAI	TTAA	2 cut(s)	38, 579
SatI	GCNGC	7 cut(s)	70, 100, 333, 336, 339, 348, 360
Sau3AI	GATC	2 cut(s)	400, 507
SchI	GAGTC	1 cut(s)	404
ScrFI	CCNGG	1 cut(s)	494
SetI	ASST	9 cut(s)	204, 296, 328, 361, 423, 471, 486, 536, 614
SfaNI	GCATC	3 cut(s)	111, 359, 579
SfcI	CTRYAG	1 cut(s)	9
SmlI	CTYRAG	1 cut(s)	73
SmoI	CTYRAG	1 cut(s)	73
SphI	GCATGC	1 cut(s)	366
SsiI	CCGC	5 cut(s)	52, 67, 97, 100, 336
SspMI	CTAG	1 cut(s)	167
StyD4I	CCNGG	1 cut(s)	492
TaaI	ACNGT	2 cut(s)	13, 598
TaqI	TCGA	4 cut(s)	108, 255, 510, 573
TauI	GCSGC	2 cut(s)	102, 338
TfiI	GAWTC	1 cut(s)	489
Tru1I	TTAA	2 cut(s)	38, 579
Tru9I	TTAA	2 cut(s)	38, 579
TscAI	CASTG	2 cut(s)	193, 601
TseI	GCWGC	5 cut(s)	69, 332, 338, 347, 359
TspDTI	ATGAA	3 cut(s)	127, 214, 333
TspGWI	ACGGA	2 cut(s)	194, 526
TspRI	CASTG	2 cut(s)	193, 601
Tth111I	GACNNNGTC	1 cut(s)	263
XceI	RCATGY	1 cut(s)	366
XspI	CTAG	1 cut(s)	167

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.