Rh3CG100600

Calcium-binding protein CML42-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0013009
Physical Location & Seq
Forward (+)
7942025 .. 7943349
1325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG100600.1

Sequence Viewer

Length: 639 bp
ATGGAAGCAGCAGCACCTTCTTCAGCCGGAACCAGTGAACGCACCCTTACCCGAAGGCCCTCAGTCTCTTCCTCCTTTCGCCTCCGCAGCCCGAGTCTTAATTCTCTCCGCCTCCTCCGCATCTTTGATGTTTTCGACAAGAACCGCGACGGCATGGTTAGCGTCGAAGAGATCAGCCAGGCCCTAGGGCTCCTCGGCCTGGACGTCGAAGTTTCCGAGCTGGAGCGCACCATCCGGACCTTCATCCAGCCCGGAAACGAAGGCCTCAAGTTCGAGGATTTCGTAGGGCTCCACCAGTCCCTTTACGATACATTCTGCTTTGACGGTGATGACATTGGAAATGCTGTGGAAGAGGCTGCGGCCGCGGTGGTGGCAGCGCGGGATGAGGAGGAGGCAAAGAAGTTGCAGGAGGAATCGGATCTAAGGGAGGCGTTCAAGGTGTTTGATGAAGATGGAGATGGGTACATATCGGCAAAGGAGCTGCAGACGGTGCTTGGGAAGCTAGGGTTTCCGGAAGGGAACGAGATTGATAGAGTGGAGAAGATGATTACTTCCGTTGACCGGAACCACGACGGCCTCGTTGACTTGTTCGAATTTAAGCACATGATGCGCAGTACCCTTCTTGTTCCTTCCTCTTGA

Protein Analysis

212

Amino Acids

23.54

Weight (kDa)

4.57

Isoelectric Point (pI)

52.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 37 - 96 1.3e-06 EF-hand domain pair
EF-hand_6 PF13405 38 - 66 4.2e-06 EF-hand domain
EF-hand_7 PF13499 138 - 204 2e-14 EF-hand domain pair
EF-hand_1 PF00036 140 - 167 3.5e-08 EF hand domain
EF-hand_6 PF13405 140 - 169 5.4e-09 EF-hand domain
EF-hand_5 PF13202 141 - 165 3.3e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20780 AT5G44460
fragaria_vesca FvH4_6g09300
malus_domestica MD04G1178900.v1.1 MD07G1157000.v1.1 MD12G1194200.v1.1
prunus_persica Prupe.6G298900_v2.0.a1
pyrus_communis pycom04g15820 pycom12g18040
rosa_chinensis RchiOBHm_Chr3g0459681
rosa_laevigata RLG00000025067
rosa_multiflora Rmu_sc0000240.1_g000021
rosa_roxburghii Rroxscaffold_6G00420080
rosa_rugosa Rorug03G0037400
rosa_samantha Rh3AG096000 Rh3BG098600 Rh3CG100600 Rh3DG100000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 207
Acc16I TGCGCA 1 cut(s) 611
AccII CGCG 3 cut(s) 147, 365, 379
AccIII TCCGGA 2 cut(s) 234, 511
AciI CCGC 8 cut(s) 85, 109, 118, 145, 359, 363, 365, 379
AclWI GGATC 1 cut(s) 426
AcoI YGGCCR 1 cut(s) 360
AcsI RAATTY 1 cut(s) 593
AcuI CTGAAG 1 cut(s) 6
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 2 cut(s) 464, 616
AfiI CCNNNNNNNGG 2 cut(s) 199, 561
AgsI TTSAA 1 cut(s) 436
AjnI CCWGG 2 cut(s) 177, 198
AluBI AGCT 3 cut(s) 220, 481, 502
AluI AGCT 3 cut(s) 220, 481, 502
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 1 cut(s) 426
Ama87I CYCGRG 1 cut(s) 91
Aor13HI TCCGGA 2 cut(s) 234, 511
AoxI GGCC 6 cut(s) 56, 180, 196, 262, 360, 574
ApeKI GCWGC 6 cut(s) 8, 11, 87, 356, 374, 481
ApoI RAATTY 1 cut(s) 593
AspA2I CCTAGG 1 cut(s) 184
AspLEI GCGC 3 cut(s) 228, 379, 612
AspS9I GGNCC 3 cut(s) 57, 181, 237
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 1 cut(s) 338
AsuII TTCGAA 1 cut(s) 591
AvaI CYCGRG 1 cut(s) 91
AvaII GGWCC 1 cut(s) 237
AvrII CCTAGG 1 cut(s) 184
BanII GRGCYC 2 cut(s) 192, 291
BbvI GCAGC 6 cut(s) 20, 23, 99, 343, 386, 468
BccI CCATC 3 cut(s) 239, 446, 452
BceAI ACGGC 2 cut(s) 166, 589
BciT130I CCWGG 2 cut(s) 179, 200
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 70
BfaI CTAG 2 cut(s) 185, 503
BfmI CTRYAG 1 cut(s) 482
BisI GCNGC 8 cut(s) 9, 12, 88, 357, 360, 363, 375, 482
BlnI CCTAGG 1 cut(s) 184
BlsI GCNGC 8 cut(s) 10, 13, 89, 358, 361, 364, 376, 483
Bme1390I CCNGG 3 cut(s) 179, 200, 252
Bme18I GGWCC 1 cut(s) 237
BmeT110I CYCGRG 1 cut(s) 91
BmgT120I GGNCC 3 cut(s) 57, 181, 237
BmiI GGNNCC 4 cut(s) 31, 191, 290, 566
BmrFI CCNGG 3 cut(s) 179, 200, 252
BmsI GCATC 2 cut(s) 129, 597
BpmI CTGGAG 1 cut(s) 242
Bpu14I TTCGAA 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 251
BpuMI CCSGG 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 3 cut(s) 184, 193, 363
BsaWI WCCGGW 3 cut(s) 234, 511, 561
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 561
Bse1I ACTGG 2 cut(s) 33, 295
BseAI TCCGGA 2 cut(s) 234, 511
BseBI CCWGG 2 cut(s) 179, 200
BseDI CCNNGG 3 cut(s) 184, 193, 363
BseGI GGATG 3 cut(s) 231, 243, 388
BseLI CCNNNNNNNGG 2 cut(s) 199, 561
BseMII CTCAG 1 cut(s) 75
BseNI ACTGG 2 cut(s) 33, 295
BseRI GAGGAG 4 cut(s) 104, 182, 401, 404
BseX3I CGGCCG 1 cut(s) 360
BseXI GCAGC 6 cut(s) 20, 23, 99, 343, 386, 468
Bsh1236I CGCG 3 cut(s) 147, 365, 379
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 6 cut(s) 58, 182, 198, 264, 362, 576
BsiEI CGRYCG 1 cut(s) 363
BsiHKCI CYCGRG 1 cut(s) 91
BsiSI CCGG 5 cut(s) 27, 235, 252, 512, 562
BslFI GGGAC 1 cut(s) 283
BslI CCNNNNNNNGG 2 cut(s) 199, 561
BsmAI GTCTC 1 cut(s) 70
BsmFI GGGAC 1 cut(s) 283
BsnI GGCC 6 cut(s) 58, 182, 198, 264, 362, 576
BsoBI CYCGRG 1 cut(s) 91
Bsp119I TTCGAA 1 cut(s) 591
Bsp1286I GDGCHC 2 cut(s) 192, 291
Bsp13I TCCGGA 2 cut(s) 234, 511
Bsp143I GATC 2 cut(s) 171, 418
BspACI CCGC 8 cut(s) 85, 109, 118, 145, 359, 363, 365, 379
BspANI GGCC 6 cut(s) 58, 182, 198, 264, 362, 576
BspCNI CTCAG 1 cut(s) 74
BspEI TCCGGA 2 cut(s) 234, 511
BspFNI CGCG 3 cut(s) 147, 365, 379
BspLI GGNNCC 4 cut(s) 31, 191, 290, 566
BspMAI CTGCAG 1 cut(s) 486
BspPI GGATC 1 cut(s) 426
BspT104I TTCGAA 1 cut(s) 591
BsrI ACTGG 2 cut(s) 33, 295
BssECI CCNNGG 3 cut(s) 184, 193, 363
BssMI GATC 2 cut(s) 171, 418
BssNI GRCGYC 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 184
Bst2UI CCWGG 2 cut(s) 179, 200
Bst4CI ACNGT 2 cut(s) 326, 490
Bst6I CTCTTC 3 cut(s) 73, 162, 345
BstACI GRCGYC 1 cut(s) 204
BstAPI GCANNNNNTGC 2 cut(s) 490, 607
BstBI TTCGAA 1 cut(s) 591
BstDEI CTNAG 2 cut(s) 61, 422
BstDSI CCRYGG 1 cut(s) 363
BstF5I GGATG 3 cut(s) 231, 243, 388
BstFNI CGCG 3 cut(s) 147, 365, 379
BstHHI GCGC 3 cut(s) 228, 379, 612
BstKTI GATC 2 cut(s) 174, 421
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 2 cut(s) 171, 418
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 8 cut(s) 87, 117, 159, 362, 371, 490, 499, 607
BstNI CCWGG 2 cut(s) 179, 200
BstSCI CCNGG 3 cut(s) 177, 198, 250
BstSFI CTRYAG 1 cut(s) 482
BstUI CGCG 3 cut(s) 147, 365, 379
BstV1I GCAGC 6 cut(s) 20, 23, 99, 343, 386, 468
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BstZI CGGCCG 1 cut(s) 360
BsuRI GGCC 6 cut(s) 58, 182, 198, 264, 362, 576
BtgI CCRYGG 1 cut(s) 363
BtsCI GGATG 3 cut(s) 231, 243, 388
BtsIMutI CAGTG 1 cut(s) 40
CciNI GCGGCCGC 1 cut(s) 360
CfoI GCGC 3 cut(s) 228, 379, 612
Cfr13I GGNCC 3 cut(s) 57, 181, 237
Cfr42I CCGCGG 1 cut(s) 366
CseI GACGC 1 cut(s) 151
Csp6I GTAC 2 cut(s) 463, 615
CviAII CATG 2 cut(s) 154, 604
CviQI GTAC 2 cut(s) 463, 615
DdeI CTNAG 2 cut(s) 61, 422
DpnI GATC 2 cut(s) 173, 420
DpnII GATC 2 cut(s) 171, 418
EaeI YGGCCR 1 cut(s) 360
EagI CGGCCG 1 cut(s) 360
Eam1104I CTCTTC 3 cut(s) 73, 162, 345
EarI CTCTTC 3 cut(s) 73, 162, 345
EciI GGCGGA 1 cut(s) 98
EclXI CGGCCG 1 cut(s) 360
Eco130I CCWWGG 1 cut(s) 184
Eco147I AGGCCT 1 cut(s) 264
Eco24I GRGCYC 2 cut(s) 192, 291
Eco47I GGWCC 1 cut(s) 237
Eco52I CGGCCG 1 cut(s) 360
Eco57I CTGAAG 1 cut(s) 6
Eco88I CYCGRG 1 cut(s) 91
EcoO109I RGGNCCY 2 cut(s) 57, 181
EcoRII CCWGG 2 cut(s) 177, 198
EcoT14I CCWWGG 1 cut(s) 184
EcoT38I GRGCYC 2 cut(s) 192, 291
ErhI CCWWGG 1 cut(s) 184
FaeI CATG 2 cut(s) 157, 607
FaiI YATR 3 cut(s) 155, 467, 605
FaqI GGGAC 1 cut(s) 283
FatI CATG 2 cut(s) 153, 603
FauI CCCGC 1 cut(s) 372
Fnu4HI GCNGC 8 cut(s) 9, 12, 88, 357, 360, 363, 375, 482
FokI GGATG 3 cut(s) 218, 230, 395
FriOI GRGCYC 2 cut(s) 192, 291
Fsp4HI GCNGC 8 cut(s) 9, 12, 88, 357, 360, 363, 375, 482
FspBI CTAG 2 cut(s) 185, 503
FspI TGCGCA 1 cut(s) 611
GlaI GCGC 3 cut(s) 227, 378, 611
GluI GCNGC 8 cut(s) 9, 12, 88, 357, 360, 363, 375, 482
GsuI CTGGAG 1 cut(s) 242
HaeIII GGCC 6 cut(s) 58, 182, 198, 264, 362, 576
HapII CCGG 5 cut(s) 27, 235, 252, 512, 562
HgaI GACGC 1 cut(s) 151
HhaI GCGC 3 cut(s) 228, 379, 612
Hin1I GRCGYC 1 cut(s) 204
Hin1II CATG 2 cut(s) 157, 607
Hin6I GCGC 3 cut(s) 226, 377, 610
HinP1I GCGC 3 cut(s) 226, 377, 610
HincII GTYRAC 2 cut(s) 559, 583
HindII GTYRAC 2 cut(s) 559, 583
HinfI GANTC 2 cut(s) 94, 413
HpaII CCGG 5 cut(s) 27, 235, 252, 512, 562
HphI GGTGA 1 cut(s) 338
Hpy166II GTNNAC 3 cut(s) 38, 559, 583
Hpy188I TCNGA 2 cut(s) 217, 418
Hpy188III TCNNGA 3 cut(s) 235, 512, 636
Hpy8I GTNNAC 3 cut(s) 38, 559, 583
Hpy99I CGWCG 4 cut(s) 152, 167, 209, 575
HpyAV CCTTC 7 cut(s) 27, 48, 250, 254, 509, 629, 639
HpyCH4III ACNGT 2 cut(s) 326, 490
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 2 cut(s) 406, 484
HpyF10VI GCNNNNNNNGC 8 cut(s) 87, 117, 159, 362, 371, 490, 499, 607
HpyF3I CTNAG 2 cut(s) 61, 422
HpySE526I ACGT 1 cut(s) 204
Hsp92I GRCGYC 1 cut(s) 204
Hsp92II CATG 2 cut(s) 157, 607
HspAI GCGC 3 cut(s) 226, 377, 610
Kpn2I TCCGGA 2 cut(s) 234, 511
KspI CCGCGG 1 cut(s) 366
Kzo9I GATC 2 cut(s) 171, 418
LmnI GCTCC 4 cut(s) 195, 223, 294, 478
Lsp1109I GCAGC 6 cut(s) 20, 23, 99, 343, 386, 468
LweI GCATC 2 cut(s) 129, 597
MaeI CTAG 2 cut(s) 185, 503
MaeII ACGT 1 cut(s) 204
MalI GATC 2 cut(s) 173, 420
MboI GATC 2 cut(s) 171, 418
MboII GAAGA 6 cut(s) 12, 60, 179, 362, 461, 553
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 2 cut(s) 192, 291
MluCI AATT 2 cut(s) 100, 593
MlyI GAGTC 1 cut(s) 103
MroI TCCGGA 2 cut(s) 234, 511
MseI TTAA 2 cut(s) 99, 597
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 5 cut(s) 27, 235, 252, 512, 562
MspR9I CCNGG 3 cut(s) 179, 200, 252
MvaI CCWGG 2 cut(s) 179, 200
MvnI CGCG 3 cut(s) 147, 365, 379
MwoI GCNNNNNNNGC 8 cut(s) 87, 117, 159, 362, 371, 490, 499, 607
NciI CCSGG 1 cut(s) 252
NdeII GATC 2 cut(s) 171, 418
NlaIII CATG 2 cut(s) 157, 607
NlaIV GGNNCC 4 cut(s) 31, 191, 290, 566
NmeAIII GCCGAG 1 cut(s) 174
NotI GCGGCCGC 1 cut(s) 360
NsbI TGCGCA 1 cut(s) 611
NspV TTCGAA 1 cut(s) 591
PceI AGGCCT 1 cut(s) 264
PcsI WCGNNNNNNNCGW 4 cut(s) 201, 213, 279, 576
PfeI GAWTC 1 cut(s) 413
PkrI GCNGC 8 cut(s) 10, 13, 89, 358, 361, 364, 376, 483
PleI GAGTC 1 cut(s) 102
PpsI GAGTC 1 cut(s) 102
Psp6I CCWGG 2 cut(s) 177, 198
PspGI CCWGG 2 cut(s) 177, 198
PspN4I GGNNCC 4 cut(s) 31, 191, 290, 566
PspPI GGNCC 3 cut(s) 57, 181, 237
PstI CTGCAG 1 cut(s) 486
PsuI RGATCY 1 cut(s) 418
RsaI GTAC 2 cut(s) 464, 616
RsaNI GTAC 2 cut(s) 463, 615
SacII CCGCGG 1 cut(s) 366
SaqAI TTAA 2 cut(s) 99, 597
SatI GCNGC 8 cut(s) 9, 12, 88, 357, 360, 363, 375, 482
Sau3AI GATC 2 cut(s) 171, 418
Sau96I GGNCC 3 cut(s) 57, 181, 237
SchI GAGTC 1 cut(s) 103
ScrFI CCNGG 3 cut(s) 179, 200, 252
SduI GDGCHC 2 cut(s) 192, 291
SetI ASST 7 cut(s) 19, 207, 222, 242, 441, 483, 504
SfaNI GCATC 2 cut(s) 129, 597
SfcI CTRYAG 1 cut(s) 482
Sfr303I CCGCGG 1 cut(s) 366
SfuI TTCGAA 1 cut(s) 591
SgrBI CCGCGG 1 cut(s) 366
SinI GGWCC 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 2 cut(s) 100, 593
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 8 cut(s) 85, 109, 118, 145, 359, 363, 365, 379
SspMI CTAG 2 cut(s) 185, 503
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 3 cut(s) 177, 198, 250
StyI CCWWGG 1 cut(s) 184
TaaI ACNGT 2 cut(s) 326, 490
TaiI ACGT 1 cut(s) 207
TaqI TCGA 5 cut(s) 135, 165, 207, 273, 591
TasI AATT 2 cut(s) 100, 593
TauI GCSGC 2 cut(s) 362, 365
TfiI GAWTC 1 cut(s) 413
Tru1I TTAA 2 cut(s) 99, 597
Tru9I TTAA 2 cut(s) 99, 597
TscAI CASTG 1 cut(s) 40
TseI GCWGC 6 cut(s) 8, 11, 87, 356, 374, 481
TspDTI ATGAA 2 cut(s) 232, 462
TspGWI ACGGA 1 cut(s) 544
TspRI CASTG 1 cut(s) 40
VpaK11BI GGWCC 1 cut(s) 237
XapI RAATTY 1 cut(s) 593
XmaJI CCTAGG 1 cut(s) 184
XspI CTAG 2 cut(s) 185, 503
ZraI GACGTC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.