AT5G44460

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013009
Physical Location & Seq
Forward (+)
17916993 .. 17918001
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44460.1

Sequence Viewer

Length: 546 bp
ATGGAGATCAATAACGAGAAGAAGAAACTCTCAAGACAATCTTCTTCCTTCAGACTACGAAGTCCAAGTCTAAACGCTCTTCGTCTCCACCGAGTCTTTGACCTATTCGACAAAAATAACGATGGATTCATAACCGTCGAAGAACTAAGTCAAGCACTCTCAAGACTCGGTCTCGACGCTGATTTCTCCGATCTCAAGTCAACAGTTGATTCTTTCATCAAACCGGACAAAACCGGACTCCGGTTCGATGACTTTGCTGCACTCCACAAAACCTTAGACGAATCTTTCTTTGGAGGAGAAGGAAGTTGCTGTGATGGGTCTCCGGAATCAGATCTTGAAGAAGCTTTTAATGTGTTCGATGAAGACGGAGATGGTTTTATCTCCGCTGTGGAATTGCAAAAGGTTTTGAAGAAGCTTGGATTACCTGAAGCAGGAGAGATTGAACAAGTGGAGAAGATGATTGTCTCTGTTGATAGTAATCATGATGGTCGCGTTGACTTTTTCGAATTCAAGAACATGATGCAAACTGTTGTTGTTCCTTCTTGA

Protein Analysis

181

Amino Acids

20.25

Weight (kDa)

4.59

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 28 - 87 2e-09 EF-hand domain pair
EF-hand_6 PF13405 28 - 57 1.4e-08 EF-hand domain
EF-hand_1 PF00036 29 - 56 8.5e-08 EF hand domain
EF-hand_5 PF13202 32 - 51 2e-06 EF hand
EF-hand_7 PF13499 109 - 175 7.9e-16 EF-hand domain pair
EF-hand_1 PF00036 111 - 139 1.2e-07 EF hand domain
EF-hand_6 PF13405 111 - 140 1.3e-08 EF-hand domain
EF-hand_5 PF13202 112 - 136 6.5e-06 EF hand
EF-hand_1 PF00036 150 - 176 8.2e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20780 AT5G44460
fragaria_vesca FvH4_6g09300
malus_domestica MD04G1178900.v1.1 MD07G1157000.v1.1 MD12G1194200.v1.1
prunus_persica Prupe.6G298900_v2.0.a1
pyrus_communis pycom04g15820 pycom12g18040
rosa_chinensis RchiOBHm_Chr3g0459681
rosa_laevigata RLG00000025067
rosa_multiflora Rmu_sc0000240.1_g000021
rosa_roxburghii Rroxscaffold_6G00420080
rosa_rugosa Rorug03G0037400
rosa_samantha Rh3AG096000 Rh3BG098600 Rh3CG100600 Rh3DG100000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 60
AccII CGCG 1 cut(s) 492
AccIII TCCGGA 1 cut(s) 322
AciI CCGC 1 cut(s) 384
AcsI RAATTY 1 cut(s) 506
AcuI CTGAAG 2 cut(s) 34, 447
AfiI CCNNNNNNNGG 2 cut(s) 240, 431
AgsI TTSAA 4 cut(s) 338, 409, 443, 511
AjuI GAANNNNNNNTTGG 2 cut(s) 273, 305
AluBI AGCT 2 cut(s) 344, 415
AluI AGCT 2 cut(s) 344, 415
Alw26I GTCTC 4 cut(s) 89, 176, 324, 469
Aor13HI TCCGGA 1 cut(s) 322
ApeKI GCWGC 1 cut(s) 257
ApoI RAATTY 1 cut(s) 506
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
AsuII TTCGAA 1 cut(s) 504
BbsI GAAGAC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 244
BccI CCATC 4 cut(s) 116, 308, 365, 479
BcgI CGANNNNNNTGC 2 cut(s) 236, 270
BcoDI GTCTC 4 cut(s) 89, 176, 324, 469
BglII AGATCT 1 cut(s) 331
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
BmsI GCATC 1 cut(s) 510
BpiI GAAGAC 1 cut(s) 369
Bpu14I TTCGAA 1 cut(s) 504
BpuEI CTTGAG 3 cut(s) 16, 145, 179
BsaBI GATNNNNATC 1 cut(s) 477
BsaI GGTCTC 2 cut(s) 176, 324
BsaWI WCCGGW 4 cut(s) 223, 233, 240, 322
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 431
Bse8I GATNNNNATC 1 cut(s) 477
BseAI TCCGGA 1 cut(s) 322
BseJI GATNNNNATC 1 cut(s) 477
BseLI CCNNNNNNNGG 2 cut(s) 240, 431
BseRI GAGGAG 1 cut(s) 309
BseXI GCAGC 1 cut(s) 244
BsgI GTGCAG 1 cut(s) 243
Bsh1236I CGCG 1 cut(s) 492
BsiSI CCGG 4 cut(s) 224, 234, 241, 323
BslI CCNNNNNNNGG 2 cut(s) 240, 431
BsmAI GTCTC 4 cut(s) 89, 176, 324, 469
BsmBI CGTCTC 1 cut(s) 89
Bso31I GGTCTC 2 cut(s) 176, 324
Bsp119I TTCGAA 1 cut(s) 504
Bsp13I TCCGGA 1 cut(s) 322
Bsp143I GATC 3 cut(s) 6, 190, 331
BspACI CCGC 1 cut(s) 384
BspEI TCCGGA 1 cut(s) 322
BspFNI CGCG 1 cut(s) 492
BspHI TCATGA 1 cut(s) 481
BspQI GCTCTTC 1 cut(s) 84
BspT104I TTCGAA 1 cut(s) 504
BspTNI GGTCTC 2 cut(s) 176, 324
BssMI GATC 3 cut(s) 6, 190, 331
Bst4CI ACNGT 3 cut(s) 136, 205, 529
Bst6I CTCTTC 1 cut(s) 84
BstBI TTCGAA 1 cut(s) 504
BstDEI CTNAG 2 cut(s) 146, 274
BstENI CCTNNNNNAGG 1 cut(s) 429
BstFNI CGCG 1 cut(s) 492
BstKTI GATC 3 cut(s) 9, 193, 334
BstMAI GTCTC 4 cut(s) 89, 176, 324, 469
BstMBI GATC 3 cut(s) 6, 190, 331
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
CciI TCATGA 1 cut(s) 481
CseI GACGC 1 cut(s) 185
CviAII CATG 2 cut(s) 482, 517
CviJI RGCY 2 cut(s) 344, 415
CviKI_1 RGCY 2 cut(s) 344, 415
DdeI CTNAG 2 cut(s) 146, 274
DpnI GATC 3 cut(s) 8, 192, 333
DpnII GATC 3 cut(s) 6, 190, 331
DrdI GACNNNNNNGTC 1 cut(s) 60
DseDI GACNNNNNNGTC 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco31I GGTCTC 2 cut(s) 176, 324
Eco57I CTGAAG 2 cut(s) 34, 447
EcoNI CCTNNNNNAGG 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 506
Esp3I CGTCTC 1 cut(s) 89
FaeI CATG 2 cut(s) 485, 520
FaiI YATR 3 cut(s) 131, 483, 518
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FatI CATG 2 cut(s) 481, 516
Fnu4HI GCNGC 1 cut(s) 258
Fsp4HI GCNGC 1 cut(s) 258
GluI GCNGC 1 cut(s) 258
HapII CCGG 4 cut(s) 224, 234, 241, 323
HgaI GACGC 1 cut(s) 185
Hin1II CATG 2 cut(s) 485, 520
HincII GTYRAC 2 cut(s) 201, 496
HindII GTYRAC 2 cut(s) 201, 496
HindIII AAGCTT 2 cut(s) 342, 413
HinfI GANTC 7 cut(s) 93, 126, 165, 209, 237, 281, 326
HpaII CCGG 4 cut(s) 224, 234, 241, 323
Hpy166II GTNNAC 2 cut(s) 201, 496
Hpy188I TCNGA 3 cut(s) 53, 190, 331
Hpy188III TCNNGA 8 cut(s) 33, 162, 173, 323, 335, 482, 511, 543
Hpy8I GTNNAC 2 cut(s) 201, 496
Hpy99I CGWCG 2 cut(s) 140, 179
HpyAV CCTTC 2 cut(s) 58, 293
HpyCH4III ACNGT 3 cut(s) 136, 205, 529
HpyCH4V TGCA 3 cut(s) 260, 397, 523
HpyF3I CTNAG 2 cut(s) 146, 274
Hsp92II CATG 2 cut(s) 485, 520
Kpn2I TCCGGA 1 cut(s) 322
Kzo9I GATC 3 cut(s) 6, 190, 331
LguI GCTCTTC 1 cut(s) 84
LpnPI CCDG 6 cut(s) 237, 247, 254, 336, 417, 438
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 1 cut(s) 510
MalI GATC 3 cut(s) 8, 192, 333
MboI GATC 3 cut(s) 6, 190, 331
MflI RGATCY 1 cut(s) 331
MluCI AATT 2 cut(s) 392, 506
MlyI GAGTC 3 cut(s) 102, 159, 231
MnlI CCTC 1 cut(s) 287
MroI TCCGGA 1 cut(s) 322
MseI TTAA 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 224, 234, 241, 323
MvnI CGCG 1 cut(s) 492
NdeII GATC 3 cut(s) 6, 190, 331
NlaIII CATG 2 cut(s) 485, 520
NspV TTCGAA 1 cut(s) 504
PagI TCATGA 1 cut(s) 481
PciSI GCTCTTC 1 cut(s) 84
PcsI WCGNNNNNNNCGW 1 cut(s) 88
PfeI GAWTC 4 cut(s) 126, 209, 281, 326
PflFI GACNNNGTC 1 cut(s) 168
PkrI GCNGC 1 cut(s) 259
PleI GAGTC 3 cut(s) 101, 159, 231
PpsI GAGTC 3 cut(s) 101, 159, 231
PsuI RGATCY 1 cut(s) 331
PsyI GACNNNGTC 1 cut(s) 168
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 3 cut(s) 6, 190, 331
SchI GAGTC 3 cut(s) 102, 159, 231
SetI ASST 6 cut(s) 105, 275, 346, 405, 417, 427
SfaNI GCATC 1 cut(s) 510
SfuI TTCGAA 1 cut(s) 504
SmlI CTYRAG 3 cut(s) 31, 160, 194
SmoI CTYRAG 3 cut(s) 31, 160, 194
Sse9I AATT 2 cut(s) 392, 506
SsiI CCGC 1 cut(s) 384
TaaI ACNGT 3 cut(s) 136, 205, 529
TaqI TCGA 6 cut(s) 108, 138, 174, 246, 357, 504
TaqII GACCGA 1 cut(s) 158
TasI AATT 2 cut(s) 392, 506
TfiI GAWTC 4 cut(s) 126, 209, 281, 326
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 3 cut(s) 118, 205, 375
TspGWI ACGGA 1 cut(s) 381
Tth111I GACNNNGTC 1 cut(s) 168
XagI CCTNNNNNAGG 1 cut(s) 429
XapI RAATTY 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.