pycom12g18040

Calcium-binding protein CML42-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013009
Physical Location & Seq
Reverse (-)
20332723 .. 20333343
621 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g18040.1

Sequence Viewer

Length: 621 bp
ATGGCCTCTACAGTTTCAGGGACTGGCGCTCCTTCGTTCAAACGGCCTTCGCCGTCGTTTCGCCTCCGCTGTACTAGTCTCAACTCTCTCCGGCTCCGCCGCATCTTCGATATGTACGACAAGAACAAGGACGGCTTCATCAGCGTCCACGAGATTAGCCAAGCCCTAGCCCTTCTCGGGTTGGACACTGAAATCTCCGACCTCCATTCCACTATAAAATCCTTCATCCAACCCTCAAACGACGGCCTCAACTTCGACGACTTCGTCTCTCTCCACCAATCCCTCTATGACACCTATTTCGACAATAACAACGGCGATGAAGAGGCTGTCGCTGCCGCTGCTGAGGCAGCATCAACAGCTGCATGCGACAAGGAGACTATGTTGTCGCAGGAGGAGTCTGATCTTTCGGAGGCGTTCAAGGTGTTTGATGAGGACGGCGACGGGTACATATCGGCTAAAGAGTTGCAGGTGGTGCTTGCCAAGCTCGGATTCCAGGAAAGCAACGAGATCGATAGAGTTGAGAAGATGATCACCTCCGTTGACCAGAACCACGACGGCCTCGTTGACTTGTTCGAGTTCAAGAACATGATGCGCACTACTGTTCTTGTTCCCAGCTCTTGA

Protein Analysis

207

Amino Acids

22.8

Weight (kDa)

4.46

Isoelectric Point (pI)

48.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 31 - 90 2.9e-08 EF-hand domain pair
EF-hand_6 PF13405 31 - 60 5e-08 EF-hand domain
EF-hand_1 PF00036 32 - 57 9.2e-07 EF hand domain
EF-hand_7 PF13499 132 - 198 3.1e-14 EF-hand domain pair
EF-hand_1 PF00036 135 - 162 1.1e-07 EF hand domain
EF-hand_6 PF13405 135 - 163 7.4e-08 EF-hand domain
EF-hand_5 PF13202 138 - 158 3.9e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013009)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G20780 AT5G44460
fragaria_vesca FvH4_6g09300
malus_domestica MD04G1178900.v1.1 MD07G1157000.v1.1 MD12G1194200.v1.1
prunus_persica Prupe.6G298900_v2.0.a1
pyrus_communis pycom04g15820 pycom12g18040
rosa_chinensis RchiOBHm_Chr3g0459681
rosa_laevigata RLG00000025067
rosa_multiflora Rmu_sc0000240.1_g000021
rosa_roxburghii Rroxscaffold_6G00420080
rosa_rugosa Rorug03G0037400
rosa_samantha Rh3AG096000 Rh3BG098600 Rh3CG100600 Rh3DG100000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 457
AasI GACNNNNNNGTC 2 cut(s) 263, 382
Acc16I TGCGCA 1 cut(s) 593
Acc36I ACCTGC 1 cut(s) 457
AciI CCGC 4 cut(s) 67, 97, 100, 336
AfaI GTAC 3 cut(s) 73, 116, 446
AfiI CCNNNNNNNGG 1 cut(s) 177
AgsI TTSAA 3 cut(s) 40, 418, 580
AhlI ACTAGT 1 cut(s) 74
AjnI CCWGG 1 cut(s) 492
AjuI GAANNNNNNNTTGG 2 cut(s) 473, 505
AluBI AGCT 3 cut(s) 359, 484, 615
AluI AGCT 3 cut(s) 359, 484, 615
Alw26I GTCTC 3 cut(s) 83, 271, 368
AlwNI CAGNNNCTG 1 cut(s) 23
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 4 cut(s) 3, 44, 244, 556
ApeKI GCWGC 4 cut(s) 332, 338, 347, 359
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AspLEI GCGC 2 cut(s) 29, 594
AsuHPI GGTGA 1 cut(s) 523
AvaI CYCGRG 1 cut(s) 176
BauI CACGAG 1 cut(s) 149
BbvCI CCTCAGC 1 cut(s) 342
BbvI GCAGC 4 cut(s) 319, 325, 346, 359
BceAI ACGGC 7 cut(s) 37, 59, 148, 259, 328, 451, 571
BcgI CGANNNNNNTGC 2 cut(s) 490, 524
BciT130I CCWGG 1 cut(s) 494
BclI TGATCA 1 cut(s) 528
BcoDI GTCTC 3 cut(s) 83, 271, 368
BcuI ACTAGT 1 cut(s) 74
BfaI CTAG 2 cut(s) 75, 167
BfmI CTRYAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 30
BfuAI ACCTGC 1 cut(s) 457
BisI GCNGC 6 cut(s) 100, 333, 336, 339, 348, 360
BlsI GCNGC 6 cut(s) 101, 334, 337, 340, 349, 361
Bme1390I CCNGG 1 cut(s) 494
BmeT110I CYCGRG 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 494
BmsI GCATC 3 cut(s) 111, 359, 579
Bpu10I CCTNAGC 1 cut(s) 342
Bsa29I ATCGAT 1 cut(s) 510
BsaXI ACNNNNNCTCC 4 cut(s) 13, 43, 365, 395
Bsc4I CCNNNNNNNGG 1 cut(s) 177
Bse1I ACTGG 1 cut(s) 28
BseBI CCWGG 1 cut(s) 494
BseCI ATCGAT 1 cut(s) 510
BseGI GGATG 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 177
BseMII CTCAG 1 cut(s) 333
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 407
BseXI GCAGC 4 cut(s) 319, 325, 346, 359
BseYI CCCAGC 1 cut(s) 611
BshFI GGCC 4 cut(s) 5, 46, 246, 558
BshVI ATCGAT 1 cut(s) 510
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 177
BsmAI GTCTC 3 cut(s) 83, 271, 368
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 4 cut(s) 5, 46, 246, 558
BsoBI CYCGRG 1 cut(s) 176
Bsp143I GATC 3 cut(s) 400, 507, 528
BspACI CCGC 4 cut(s) 67, 97, 100, 336
BspANI GGCC 4 cut(s) 5, 46, 246, 558
BspCNI CTCAG 1 cut(s) 334
BspDI ATCGAT 1 cut(s) 510
BspLI GGNNCC 1 cut(s) 95
BspMI ACCTGC 1 cut(s) 457
BsrI ACTGG 1 cut(s) 28
BssMI GATC 3 cut(s) 400, 507, 528
BssSI CACGAG 1 cut(s) 149
Bst2BI CACGAG 1 cut(s) 149
Bst2UI CCWGG 1 cut(s) 494
Bst4CI ACNGT 2 cut(s) 13, 601
Bst6I CTCTTC 1 cut(s) 315
BstAPI GCANNNNNTGC 1 cut(s) 472
BstC8I GCNNGC 2 cut(s) 364, 477
BstDEI CTNAG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 225
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 2 cut(s) 29, 594
BstKTI GATC 3 cut(s) 403, 510, 531
BstMAI GTCTC 3 cut(s) 83, 271, 368
BstMBI GATC 3 cut(s) 400, 507, 528
BstMWI GCNNNNNNNGC 8 cut(s) 141, 332, 338, 344, 347, 356, 472, 481
BstNI CCWGG 1 cut(s) 494
BstNSI RCATGY 1 cut(s) 366
BstSCI CCNGG 1 cut(s) 492
BstSFI CTRYAG 1 cut(s) 9
BstV1I GCAGC 4 cut(s) 319, 325, 346, 359
Bsu15I ATCGAT 1 cut(s) 510
BsuRI GGCC 4 cut(s) 5, 46, 246, 558
BsuTUI ATCGAT 1 cut(s) 510
BtgZI GCGATG 1 cut(s) 330
BtsCI GGATG 1 cut(s) 225
BtsIMutI CAGTG 1 cut(s) 186
BveI ACCTGC 1 cut(s) 457
Cac8I GCNNGC 2 cut(s) 364, 477
CaiI CAGNNNCTG 1 cut(s) 23
CfoI GCGC 2 cut(s) 29, 594
ClaI ATCGAT 1 cut(s) 510
CseI GACGC 1 cut(s) 133
Csp6I GTAC 3 cut(s) 72, 115, 445
CviAII CATG 2 cut(s) 363, 586
CviQI GTAC 3 cut(s) 72, 115, 445
DdeI CTNAG 1 cut(s) 342
DpnI GATC 3 cut(s) 402, 509, 530
DpnII GATC 3 cut(s) 400, 507, 528
DrdI GACNNNNNNGTC 2 cut(s) 263, 382
DseDI GACNNNNNNGTC 2 cut(s) 263, 382
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
EciI GGCGGA 1 cut(s) 86
Eco88I CYCGRG 1 cut(s) 176
EcoRII CCWGG 1 cut(s) 492
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 2 cut(s) 366, 589
FaiI YATR 7 cut(s) 113, 215, 288, 364, 380, 449, 587
FalI AAGNNNNNCTT 2 cut(s) 119, 151
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 362, 585
FbaI TGATCA 1 cut(s) 528
Fnu4HI GCNGC 6 cut(s) 100, 333, 336, 339, 348, 360
FokI GGATG 1 cut(s) 212
Fsp4HI GCNGC 6 cut(s) 100, 333, 336, 339, 348, 360
FspAI RTGCGCAY 1 cut(s) 593
FspBI CTAG 2 cut(s) 75, 167
FspI TGCGCA 1 cut(s) 593
GlaI GCGC 2 cut(s) 28, 593
GluI GCNGC 6 cut(s) 100, 333, 336, 339, 348, 360
GsaI CCCAGC 1 cut(s) 615
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 4 cut(s) 5, 46, 246, 558
HapII CCGG 1 cut(s) 91
HgaI GACGC 1 cut(s) 133
HhaI GCGC 2 cut(s) 29, 594
Hin1II CATG 2 cut(s) 366, 589
Hin6I GCGC 2 cut(s) 27, 592
HinP1I GCGC 2 cut(s) 27, 592
HincII GTYRAC 2 cut(s) 541, 565
HindII GTYRAC 2 cut(s) 541, 565
HinfI GANTC 2 cut(s) 395, 489
HpaII CCGG 1 cut(s) 91
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 3 cut(s) 148, 541, 565
Hpy188I TCNGA 4 cut(s) 199, 400, 409, 488
Hpy188III TCNNGA 2 cut(s) 580, 618
Hpy8I GTNNAC 3 cut(s) 148, 541, 565
Hpy99I CGWCG 5 cut(s) 58, 245, 260, 443, 557
HpyAV CCTTC 4 cut(s) 42, 57, 182, 232
HpyCH4III ACNGT 2 cut(s) 13, 601
HpyCH4V TGCA 2 cut(s) 362, 466
HpyF10VI GCNNNNNNNGC 8 cut(s) 141, 332, 338, 344, 347, 356, 472, 481
HpyF3I CTNAG 1 cut(s) 342
Hsp92II CATG 2 cut(s) 366, 589
HspAI GCGC 2 cut(s) 27, 592
Ksp22I TGATCA 1 cut(s) 528
Kzo9I GATC 3 cut(s) 400, 507, 528
LmnI GCTCC 2 cut(s) 34, 99
LpnPI CCDG 8 cut(s) 3, 9, 104, 374, 452, 479, 506, 557
Lsp1109I GCAGC 4 cut(s) 319, 325, 346, 359
LweI GCATC 3 cut(s) 111, 359, 579
MaeI CTAG 2 cut(s) 75, 167
MalI GATC 3 cut(s) 402, 509, 530
MboI GATC 3 cut(s) 400, 507, 528
MboII GAAGA 3 cut(s) 97, 332, 535
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 3 cut(s) 162, 222, 253
MspA1I CMGCKG 3 cut(s) 69, 338, 359
MspI CCGG 1 cut(s) 91
MspR9I CCNGG 1 cut(s) 494
MvaI CCWGG 1 cut(s) 494
MwoI GCNNNNNNNGC 8 cut(s) 141, 332, 338, 344, 347, 356, 472, 481
NdeII GATC 3 cut(s) 400, 507, 528
NlaIII CATG 2 cut(s) 366, 589
NlaIV GGNNCC 1 cut(s) 95
NsbI TGCGCA 1 cut(s) 593
NspI RCATGY 1 cut(s) 366
PaeI GCATGC 1 cut(s) 366
PaqCI CACCTGC 1 cut(s) 457
PcsI WCGNNNNNNNCGW 3 cut(s) 114, 261, 558
PfeI GAWTC 1 cut(s) 489
PflFI GACNNNGTC 1 cut(s) 263
PfoI TCCNGGA 1 cut(s) 492
PkrI GCNGC 6 cut(s) 101, 334, 337, 340, 349, 361
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
Psp6I CCWGG 1 cut(s) 492
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 1 cut(s) 492
PspN4I GGNNCC 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 23
PsyI GACNNNGTC 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 3 cut(s) 73, 116, 446
RsaNI GTAC 3 cut(s) 72, 115, 445
SatI GCNGC 6 cut(s) 100, 333, 336, 339, 348, 360
Sau3AI GATC 3 cut(s) 400, 507, 528
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 494
SetI ASST 8 cut(s) 204, 296, 361, 423, 471, 486, 536, 617
SfaNI GCATC 3 cut(s) 111, 359, 579
SfcI CTRYAG 1 cut(s) 9
SpeI ACTAGT 1 cut(s) 74
SphI GCATGC 1 cut(s) 366
SsiI CCGC 4 cut(s) 67, 97, 100, 336
SspMI CTAG 2 cut(s) 75, 167
StyD4I CCNGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 13, 601
TaqI TCGA 5 cut(s) 108, 255, 300, 510, 573
TatI WGTACW 1 cut(s) 71
TauI GCSGC 2 cut(s) 102, 338
TfiI GAWTC 1 cut(s) 489
TscAI CASTG 1 cut(s) 193
TseI GCWGC 4 cut(s) 332, 338, 347, 359
TspDTI ATGAA 3 cut(s) 127, 214, 333
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 1 cut(s) 193
Tth111I GACNNNGTC 1 cut(s) 263
XceI RCATGY 1 cut(s) 366
XspI CTAG 2 cut(s) 75, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.