AT4G21065

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0013500
Physical Location & Seq
Forward (+)
11245790 .. 11247807
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G21065.1

Sequence Viewer

Length: 1788 bp
ATGTCTCCATTCTCAGAAACCTCTGTTCTGCTACTTCCCATGGTCGAGAAATGCATAAACCTCTTGCAAACCTACGGCGTTTCATCGATAACGAAACTCAGACAAATCCACGCTTTTTCAATCCGCCATGGAGTCTCAATCTCAGACGCCGAGCTTGGGAAACACCTCATCTTCTACCTTGTTTCTCTTCCTTCTCCACCTCCCATGTCTTACGCTCACAAAGTCTTCTCCAAGATTGAGAAACCCATCAATGTCTTTATTTGGAACACATTGATCAGAGGCTACGCCGAGATTGGTAATTCCATCTCTGCTTTTTCTCTATACCGTGAGATGAGAGTCTCTGGTTTGGTCGAGCCAGATACTCATACTTATCCGTTTCTTATTAAGGCTGTGACGACAATGGCGGATGTTAGATTAGGAGAGACGATTCATTCGGTTGTTATAAGAAGTGGCTTTGGTTCGTTGATCTATGTTCAGAACTCTCTGCTTCATTTATACGCAAATTGTGGAGATGTAGCTAGTGCGTACAAGGTGTTTGATAAAATGCCTGAGAAAGATTTAGTGGCTTGGAACTCTGTGATTAATGGGTTTGCGGAAAACGGTAAACCAGAAGAAGCTTTAGCGCTTTATACTGAGATGAATTCCAAGGGTATCAAGCCTGATGGGTTCACAATTGTTAGCTTGTTGTCTGCTTGTGCAAAGATTGGTGCTTTGACATTGGGTAAGAGAGTTCATGTCTATATGATCAAAGTAGGTTTGACACGAAACTTGCATTCCAGCAATGTGCTCTTGGACTTGTATGCGAGATGTGGAAGAGTGGAAGAAGCCAAAACACTTTTCGATGAAATGGTGGACAAGAACAGTGTCTCGTGGACTTCGTTGATAGTCGGTTTAGCTGTTAATGGTTTTGGGAAAGAAGCCATTGAGCTTTTCAAGTATATGGAATCAACGGAGGGATTGTTACCATGTGAGATCACGTTTGTTGGGATATTATACGCTTGTAGCCATTGCGGGATGGTGAAGGAAGGTTTTGAGTATTTCAGGAGAATGAGAGAGGAGTATAAGATTGAACCTAGGATCGAGCATTTTGGTTGTATGGTCGATTTGTTAGCTAGAGCTGGACAAGTGAAGAAAGCATATGAATACATCAAGAGTATGCCGATGCAGCCTAATGTTGTAATCTGGAGAACATTGTTAGGAGCTTGTACTGTTCATGGTGATTCAGATTTAGCAGAGTTTGCTAGAATCCAGATCTTACAACTAGAACCAAACCACAGCGGCGATTATGTTCTCTTGTCGAATATGTACGCATCCGAGCAGCGTTGGTCCGATGTGCAAAAGATACGGAAACAAATGTTAAGAGACGGTGTGAAAAAAGTCCCCGGTCACAGCTTAGTAGAAGTAGGAAACAGAGTTCATGAGTTTCTTATGGGTGATAAGTCTCACCCACAAAGCGATGCTATTTACGCTAAGCTGAAAGAAATGACTGGTAGATTGAGATCAGAAGGTTACGTTCCACAAATTTCTAATGTCTATGTAGATGTTGAAGAGGAAGAGAAGGAGAATGCTGTGGTTTATCACAGTGAGAAGATTGCTATTGCATTTATGTTAATTAGTACACCAGAAAGATCACCGATTACAGTCGTGAAGAATCTTAGAGTATGTGCAGATTGTCACTTAGCAATCAAACTTGTGTCAAAGGTTTATAACCGAGAGATTGTAGTCAGAGATCGTAGCAGGTTTCACCATTTTAAAAATGGTTCTTGTTCTTGCCAAGATTACTGGTAG

Protein Analysis

595

Amino Acids

67.1

Weight (kDa)

8.1

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 84 - 130 8.9e-10 PPR repeat family
PPR PF01535 87 - 116 3.5e-06 PPR repeat
PPR_2 PF13041 186 - 234 2.4e-14 PPR repeat family
PPR PF01535 188 - 218 3e-08 PPR repeat
PPR_3 PF13812 209 - 267 3.3e-06 Pentatricopeptide repeat domain
PPR PF01535 261 - 287 3e-06 PPR repeat
E_motif PF20431 405 - 467 2.8e-17 E motif
Eplus_motif PF20430 472 - 499 7.6e-09 E+ motif
DYW_deaminase PF14432 503 - 595 8.4e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21065 AT4G21065
fragaria_vesca FvH4_3g09500
malus_domestica MD05G1277300.v1.1 MD10G1256200.v1.1
prunus_persica Prupe.4G087400_v2.0.a1
pyrus_communis pycom05g25840 pycom10g21200
rosa_chinensis RchiOBHm_Chr5g0015081
rosa_laevigata RLG00000032168
rosa_multiflora Rmu_co8160296.1_g000001 Rmu_sc0002209.1_g000002 Rmu_sc0009777.1_g000014 Rmu_sc0036572.1_g000001
rosa_roxburghii Rroxscaffold_1G00061450
rosa_rugosa Rorug05G0024200
rosa_samantha Rh5CG127500
rosa_wichuraiana Rw5G010250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 443, 1707
Acc36I ACCTGC 1 cut(s) 1728
AciI CCGC 5 cut(s) 124, 404, 593, 1011, 1278
AclWI GGATC 1 cut(s) 1085
AcsI RAATTY 2 cut(s) 640, 1521
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 4 cut(s) 527, 1207, 1307, 1618
AfeI AGCGCT 1 cut(s) 624
AfiI CCNNNNNNNGG 1 cut(s) 156
AgsI TTSAA 4 cut(s) 120, 934, 1070, 1547
AjuI GAANNNNNNNTTGG 2 cut(s) 821, 853
AloI GAACNNNNNNTCC 2 cut(s) 1398, 1430
Alw21I GWGCWC 1 cut(s) 789
Alw26I GTCTC 7 cut(s) 9, 139, 343, 416, 871, 1356, 1446
AlwI GGATC 1 cut(s) 1085
Aor51HI AGCGCT 1 cut(s) 624
ApeKI GCWGC 2 cut(s) 1165, 1318
ApoI RAATTY 2 cut(s) 640, 1521
ArsI GACNNNNNNTTYG 2 cut(s) 415, 447
AseI ATTAAT 1 cut(s) 582
AspA2I CCTAGG 1 cut(s) 1073
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 1 cut(s) 1326
AsuC2I CCSGG 1 cut(s) 1383
AsuHPI GGTGA 6 cut(s) 1030, 1229, 1436, 1445, 1623, 1736
AvaII GGWCC 1 cut(s) 1326
AvrII CCTAGG 1 cut(s) 1073
BauI CACGAG 1 cut(s) 868
BbsI GAAGAC 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 789
BbvI GCAGC 2 cut(s) 1177, 1330
BccI CCATC 4 cut(s) 254, 311, 656, 1009
BceAI ACGGC 1 cut(s) 91
BclI TGATCA 2 cut(s) 273, 744
BcnI CCSGG 1 cut(s) 1383
BcoDI GTCTC 7 cut(s) 9, 139, 343, 416, 871, 1356, 1446
BfaI CTAG 5 cut(s) 519, 1074, 1113, 1242, 1262
BfoI RGCGCY 1 cut(s) 626
BfuAI ACCTGC 1 cut(s) 1728
BglII AGATCT 1 cut(s) 1251
BisI GCNGC 3 cut(s) 1166, 1279, 1319
BlnI CCTAGG 1 cut(s) 1073
BlpI GCTNAGC 1 cut(s) 1470
BlsI GCNGC 3 cut(s) 1167, 1280, 1320
Bme1390I CCNGG 1 cut(s) 1383
Bme18I GGWCC 1 cut(s) 1326
BmgT120I GGNCC 1 cut(s) 1326
BmrFI CCNGG 1 cut(s) 1383
BmsI GCATC 3 cut(s) 1152, 1319, 1447
BpiI GAAGAC 1 cut(s) 217
BpmI CTGGAG 1 cut(s) 1204
Bpu1102I GCTNAGC 1 cut(s) 1470
BpuMI CCSGG 1 cut(s) 1383
Bsa29I ATCGAT 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 1498
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 5 cut(s) 39, 127, 645, 1073, 1381
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse1I ACTGG 2 cut(s) 1492, 1787
Bse3DI GCAATG 2 cut(s) 787, 1006
Bse8I GATNNNNATC 1 cut(s) 1498
BseCI ATCGAT 1 cut(s) 86
BseDI CCNNGG 5 cut(s) 39, 127, 645, 1073, 1381
BseGI GGATG 3 cut(s) 412, 1020, 1310
BseJI GATNNNNATC 1 cut(s) 1498
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 2 cut(s) 787, 1006
BseMII CTCAG 5 cut(s) 27, 112, 156, 540, 624
BseNI ACTGG 2 cut(s) 1492, 1787
BseRI GAGGAG 1 cut(s) 1070
BseXI GCAGC 2 cut(s) 1177, 1330
BsgI GTGCAG 1 cut(s) 1686
BshVI ATCGAT 1 cut(s) 86
BsiHKAI GWGCWC 1 cut(s) 789
BsiSI CCGG 1 cut(s) 1383
BslFI GGGAC 1 cut(s) 1364
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 7 cut(s) 9, 139, 343, 416, 871, 1356, 1446
BsmBI CGTCTC 2 cut(s) 416, 1356
BsmFI GGGAC 1 cut(s) 1364
BsmI GAATGC 2 cut(s) 772, 1570
Bsp1286I GDGCHC 1 cut(s) 789
Bsp143I GATC 9 cut(s) 273, 465, 744, 972, 1077, 1251, 1499, 1628, 1729
Bsp1720I GCTNAGC 1 cut(s) 1470
Bsp19I CCATGG 2 cut(s) 39, 127
BspACI CCGC 5 cut(s) 124, 404, 593, 1011, 1278
BspCNI CTCAG 5 cut(s) 26, 111, 155, 541, 625
BspDI ATCGAT 1 cut(s) 86
BspHI TCATGA 1 cut(s) 1417
BspMI ACCTGC 1 cut(s) 1728
BspPI GGATC 1 cut(s) 1085
BsrDI GCAATG 2 cut(s) 787, 1006
BsrI ACTGG 2 cut(s) 1492, 1787
BssECI CCNNGG 5 cut(s) 39, 127, 645, 1073, 1381
BssMI GATC 9 cut(s) 273, 465, 744, 972, 1077, 1251, 1499, 1628, 1729
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 868
BssT1I CCWWGG 4 cut(s) 39, 127, 645, 1073
Bst2BI CACGAG 1 cut(s) 868
Bst4CI ACNGT 7 cut(s) 326, 602, 863, 1210, 1367, 1583, 1642
Bst6I CTCTTC 4 cut(s) 192, 808, 1542, 1548
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 1238
BstDEI CTNAG 9 cut(s) 13, 98, 142, 549, 633, 1393, 1470, 1655, 1678
BstDSI CCRYGG 2 cut(s) 39, 127
BstF5I GGATG 3 cut(s) 412, 1020, 1310
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 9 cut(s) 276, 468, 747, 975, 1080, 1254, 1502, 1631, 1732
BstMAI GTCTC 7 cut(s) 9, 139, 343, 416, 871, 1356, 1446
BstMBI GATC 9 cut(s) 273, 465, 744, 972, 1077, 1251, 1499, 1628, 1729
BstMWI GCNNNNNNNGC 3 cut(s) 1165, 1238, 1466
BstSCI CCNGG 1 cut(s) 1381
BstV1I GCAGC 2 cut(s) 1177, 1330
BstV2I GAAGAC 1 cut(s) 217
BstX2I RGATCY 1 cut(s) 1251
BstYI RGATCY 1 cut(s) 1251
Bsu15I ATCGAT 1 cut(s) 86
BsuTUI ATCGAT 1 cut(s) 86
BtgI CCRYGG 2 cut(s) 39, 127
BtgZI GCGATG 1 cut(s) 1470
BtsCI GGATG 3 cut(s) 412, 1020, 1310
BtsIMutI CAGTG 2 cut(s) 868, 1588
BveI ACCTGC 1 cut(s) 1728
CciI TCATGA 1 cut(s) 1417
CfoI GCGC 1 cut(s) 625
Cfr13I GGNCC 1 cut(s) 1326
ClaI ATCGAT 1 cut(s) 86
CseI GACGC 1 cut(s) 155
Csp6I GTAC 4 cut(s) 526, 1206, 1306, 1617
CviAII CATG 7 cut(s) 40, 128, 205, 734, 966, 1214, 1418
CviQI GTAC 4 cut(s) 526, 1206, 1306, 1617
DdeI CTNAG 9 cut(s) 13, 98, 142, 549, 633, 1393, 1470, 1655, 1678
DpnI GATC 9 cut(s) 275, 467, 746, 974, 1079, 1253, 1501, 1630, 1731
DpnII GATC 9 cut(s) 273, 465, 744, 972, 1077, 1251, 1499, 1628, 1729
DraI TTTAAA 1 cut(s) 1753
Eam1104I CTCTTC 4 cut(s) 192, 808, 1542, 1548
EarI CTCTTC 4 cut(s) 192, 808, 1542, 1548
EciI GGCGGA 2 cut(s) 113, 419
Eco130I CCWWGG 4 cut(s) 39, 127, 645, 1073
Eco47I GGWCC 1 cut(s) 1326
Eco47III AGCGCT 1 cut(s) 624
EcoRI GAATTC 1 cut(s) 640
EcoT14I CCWWGG 4 cut(s) 39, 127, 645, 1073
EcoT22I ATGCAT 1 cut(s) 56
ErhI CCWWGG 4 cut(s) 39, 127, 645, 1073
Esp3I CGTCTC 2 cut(s) 416, 1356
FaeI CATG 7 cut(s) 43, 131, 208, 737, 969, 1217, 1421
FaqI GGGAC 1 cut(s) 1364
FatI CATG 7 cut(s) 39, 127, 204, 733, 965, 1213, 1417
FauI CCCGC 1 cut(s) 1004
FauNDI CATATG 1 cut(s) 1138
FbaI TGATCA 2 cut(s) 273, 744
Fnu4HI GCNGC 3 cut(s) 1166, 1279, 1319
FokI GGATG 3 cut(s) 419, 1027, 1297
Fsp4HI GCNGC 3 cut(s) 1166, 1279, 1319
FspBI CTAG 5 cut(s) 519, 1074, 1113, 1242, 1262
GlaI GCGC 1 cut(s) 624
GluI GCNGC 3 cut(s) 1166, 1279, 1319
GsuI CTGGAG 1 cut(s) 1204
HaeII RGCGCY 1 cut(s) 626
HapII CCGG 1 cut(s) 1383
HgaI GACGC 1 cut(s) 155
HhaI GCGC 1 cut(s) 625
Hin1I GRCGYC 1 cut(s) 147
Hin1II CATG 7 cut(s) 43, 131, 208, 737, 969, 1217, 1421
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HindIII AAGCTT 1 cut(s) 615
HinfI GANTC 7 cut(s) 132, 336, 427, 944, 1220, 1245, 1651
HpaII CCGG 1 cut(s) 1383
HphI GGTGA 6 cut(s) 1030, 1229, 1436, 1445, 1623, 1736
Hpy166II GTNNAC 5 cut(s) 605, 669, 853, 873, 1619
Hpy188III TCNNGA 7 cut(s) 46, 1042, 1150, 1183, 1249, 1418, 1645
Hpy8I GTNNAC 5 cut(s) 605, 669, 853, 873, 1619
HpyAV CCTTC 5 cut(s) 201, 1015, 1019, 1499, 1552
HpyCH4III ACNGT 7 cut(s) 326, 602, 863, 1210, 1367, 1583, 1642
HpyCH4IV ACGT 2 cut(s) 977, 1512
HpyCH4V TGCA 8 cut(s) 54, 67, 698, 772, 1165, 1336, 1601, 1667
HpyF10VI GCNNNNNNNGC 3 cut(s) 1165, 1238, 1466
HpyF3I CTNAG 9 cut(s) 13, 98, 142, 549, 633, 1393, 1470, 1655, 1678
HpySE526I ACGT 2 cut(s) 977, 1512
Hsp92I GRCGYC 1 cut(s) 147
Hsp92II CATG 7 cut(s) 43, 131, 208, 737, 969, 1217, 1421
HspAI GCGC 1 cut(s) 623
Ksp22I TGATCA 2 cut(s) 273, 744
Kzo9I GATC 9 cut(s) 273, 465, 744, 972, 1077, 1251, 1499, 1628, 1729
LmnI GCTCC 1 cut(s) 1199
Lsp1109I GCAGC 2 cut(s) 1177, 1330
LweI GCATC 3 cut(s) 1152, 1319, 1447
MaeI CTAG 5 cut(s) 519, 1074, 1113, 1242, 1262
MaeII ACGT 2 cut(s) 977, 1512
MaeIII GTNAC 5 cut(s) 391, 960, 1385, 1508, 1673
MalI GATC 9 cut(s) 275, 467, 746, 974, 1079, 1253, 1501, 1630, 1731
MboI GATC 9 cut(s) 273, 465, 744, 972, 1077, 1251, 1499, 1628, 1729
MfeI CAATTG 1 cut(s) 672
MflI RGATCY 1 cut(s) 1251
MhlI GDGCHC 1 cut(s) 789
MluCI AATT 6 cut(s) 298, 502, 640, 672, 1521, 1611
MlyI GAGTC 2 cut(s) 141, 345
MnlI CCTC 8 cut(s) 31, 71, 176, 210, 272, 946, 1048, 1543
Mph1103I ATGCAT 1 cut(s) 56
MseI TTAA 6 cut(s) 384, 582, 900, 1358, 1610, 1752
MspA1I CMGCKG 1 cut(s) 1278
MspI CCGG 1 cut(s) 1383
MspR9I CCNGG 1 cut(s) 1383
MunI CAATTG 1 cut(s) 672
Mva1269I GAATGC 2 cut(s) 772, 1570
MwoI GCNNNNNNNGC 3 cut(s) 1165, 1238, 1466
NciI CCSGG 1 cut(s) 1383
NcoI CCATGG 2 cut(s) 39, 127
NdeI CATATG 1 cut(s) 1138
NdeII GATC 9 cut(s) 273, 465, 744, 972, 1077, 1251, 1499, 1628, 1729
NlaIII CATG 7 cut(s) 43, 131, 208, 737, 969, 1217, 1421
NmeAIII GCCGAG 2 cut(s) 175, 313
NmuCI GTSAC 3 cut(s) 391, 1385, 1673
NsiI ATGCAT 1 cut(s) 56
PagI TCATGA 1 cut(s) 1417
PcsI WCGNNNNNNNCGW 1 cut(s) 875
PctI GAATGC 2 cut(s) 772, 1570
PfeI GAWTC 5 cut(s) 427, 944, 1220, 1245, 1651
PkrI GCNGC 3 cut(s) 1167, 1280, 1320
PleI GAGTC 2 cut(s) 140, 344
PpsI GAGTC 2 cut(s) 140, 344
PshBI ATTAAT 1 cut(s) 582
PsiI TTATAA 2 cut(s) 443, 1707
PspPI GGNCC 1 cut(s) 1326
PsuI RGATCY 1 cut(s) 1251
RsaI GTAC 4 cut(s) 527, 1207, 1307, 1618
RsaNI GTAC 4 cut(s) 526, 1206, 1306, 1617
SaqAI TTAA 6 cut(s) 384, 582, 900, 1358, 1610, 1752
SatI GCNGC 3 cut(s) 1166, 1279, 1319
Sau3AI GATC 9 cut(s) 273, 465, 744, 972, 1077, 1251, 1499, 1628, 1729
Sau96I GGNCC 1 cut(s) 1326
SchI GAGTC 2 cut(s) 141, 345
ScrFI CCNGG 1 cut(s) 1383
SduI GDGCHC 1 cut(s) 789
SfaNI GCATC 3 cut(s) 1152, 1319, 1447
SinI GGWCC 1 cut(s) 1326
Sse9I AATT 6 cut(s) 298, 502, 640, 672, 1521, 1611
SsiI CCGC 5 cut(s) 124, 404, 593, 1011, 1278
SspMI CTAG 5 cut(s) 519, 1074, 1113, 1242, 1262
StyD4I CCNGG 1 cut(s) 1381
StyI CCWWGG 4 cut(s) 39, 127, 645, 1073
TaaI ACNGT 7 cut(s) 326, 602, 863, 1210, 1367, 1583, 1642
TaiI ACGT 2 cut(s) 980, 1515
TaqI TCGA 7 cut(s) 45, 86, 351, 840, 1080, 1101, 1298
TasI AATT 6 cut(s) 298, 502, 640, 672, 1521, 1611
TatI WGTACW 2 cut(s) 1205, 1616
TauI GCSGC 1 cut(s) 1281
TfiI GAWTC 5 cut(s) 427, 944, 1220, 1245, 1651
Tru1I TTAA 6 cut(s) 384, 582, 900, 1358, 1610, 1752
Tru9I TTAA 6 cut(s) 384, 582, 900, 1358, 1610, 1752
TscAI CASTG 2 cut(s) 868, 1588
TseFI GTSAC 3 cut(s) 391, 1385, 1673
TseI GCWGC 2 cut(s) 1165, 1318
Tsp45I GTSAC 3 cut(s) 391, 1385, 1673
TspDTI ATGAA 9 cut(s) 72, 419, 479, 653, 722, 858, 1155, 1202, 1406
TspGWI ACGGA 3 cut(s) 363, 965, 1360
TspRI CASTG 2 cut(s) 868, 1588
VpaK11BI GGWCC 1 cut(s) 1326
VspI ATTAAT 1 cut(s) 582
XapI RAATTY 2 cut(s) 640, 1521
XcmI CCANNNNNNNNNTGG 1 cut(s) 1754
XmaJI CCTAGG 1 cut(s) 1073
XspI CTAG 5 cut(s) 519, 1074, 1113, 1242, 1262
Zsp2I ATGCAT 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.