MD05G1277300.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0013500
Physical Location & Seq
Reverse (-)
41211108 .. 41213269
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1277300.v1.1.491

Sequence Viewer

Length: 2031 bp
ATGCTCCCAAAGTATTCCACACTCATCCACCCAACTTTTGCACATAAATTCTCAACCTCCTTAACTCACAACCTCAAAACCCACTTCTCTACCTTTGCACCCTCCTCACAAACTTCCCCACAAAACCCAATTGGTCACCTTCTCCAAAAATGCATTGCTTTCCTGCAATGCTGTGCATCCTCCAAGTCCAAACTGCAGCAAATCCATGCCTTCTCCATAAGGCATGGGGTACCACTCAGCAACCCAGACATGGGCAAACACCTCATCTTCTCCATAGTCTCTCTCTCTGCTCCAGTTTCCTATGCCCACCAAATATTTTCCCAAATAAAAAACCCCAATGTGTTCACATGGAACACCATGATCAGAGGCTATGCTGAGTGTCAGAACCCAGTTCCTGTCATCCAACTTTACCACCAGATGCATCAAAGTTCCGTGCAACCTGATACACACACTTACCCTTTTCTTTTGAAGGCTGTGGCTAAGTTGATGGATGTTGGAGAGGGTGAGAAGATACATTCTGTTGTCGTAAGAAATGGGTTTGAGTCATTGGTGTTTGTTAAGAACGCATTGCTTCATGTTTATGCCTGTTGTGGGCATGTTGAGAGTGCACACAAGGTGTTTGAATCAATGTCTGAGAGAGACCTTGTCGCTTGGAATTCTGTGATTAATGCGTTTGCTTTAAACGGGAGGCCGAACGAGGCTCTGACGATTTTCAGGGATATGAGTTTGGAAGGTGTTGAGCCGGATGGGTTCACCATGGTTAGCTTGTTATCTGCTTGTGCTGAGATTGGGACTTTGGCTTTGGGTAGGAGGATCCATGTGTATATGTTGAAGGTGGGATTGACGGGAAATTCGCATGCTACGAATGCGCTGCTGGATCTTTATGCAAAGTGCGGAAGCATTAGAGAGGCGCAGAAGGTGTTTGAAGTGATGGACGAAAGGAGTGTGGTTTCTTGGACTGCTTTAGTTGTTGGGTTGGCTGTTAATGGGTTTGGTATGGAAGCACTTGAGCTTTTCAAGGAGTTGAAGGGAGAGGGATTGGTGCCCACTGAGATCACTTTTGTCGGCGTATTGTATGCCTGCAGCCATTGTGGGATGGTTGATGAAGGCTTTGAATACTTTCGAATGATGAAAGAGGGGTATGGAATTGCGCCGAGAATAGAACATTATGGTTGTATGGTTGATTTGTTAGGCAGGGCGGGCTTAGTGAAACGAGCATATGAATACATTATGAACATGCCCATGAAGCCGAATGCTGTTATTTGGAGGACCTTATTAGGAGCGTGCACGATACACGGGCAGTTGGCTCTTGGGGAGACTGCAAGAGCACGCATCCGCGAGTTAGAGCCTGGACACAGTGGGGATTATGTGCTCCTCTCCAATCTCTATGCATCTGAACACCGTTGGTCAGACGTCCAAAAGGTCAGGAGGACAATGCTTAGTGAAGGGGTGAGGAAAACTCCCGGATATAGTATTGTTGAGCTGAGGAATTGTATTTATGAGTTCACTATGGGTGACAGATCTCATCCACAAAGTGAAGAGATATACGCAATGCTAGCAGAGATCGCGCGATTGTTGAAACCGAAAGGGTACGTGCCACATACGGCAAATGTTCTTGCGGACATAGAGGAGGAGGAGAAAGAAAATGCTTTGTCGTATCATAGTGAAAAGATTGCAGTTGCCTTTATGCTCCTAAATACTGCACCAGGGGTCCCGATTAGGATTTGGAAGAACTTGAGAGTTTGTGCAGATTGTCATCTTGCATTTAAACTCATATCGAAGGTTTATGATCGGGAGATTGTTGTGAGGGATCGTAGTCGGTTTCACCATTTTAGAGATGGTTCTTGTTCTTGTAGAGATTACTGTGGCGAATTCATGATTCAAATCTTAAGATGCTCGGGGGACAGGCGAAGGGAAAGGGCCCAAGGGCTGGTGGATAGGGCTCCAGTGTTGGGCAGCCCGAGTGAAGGTGGGAGCCTGAGGGCCAGGTCCGTGGAGGATTGTCAGCCCTGCAGTCCGAGGTGGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 X8

Protein Analysis

677

Amino Acids

75.69

Weight (kDa)

8.26

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 112 - 161 7.8e-12 PPR repeat family
PPR_2 PF13041 214 - 261 1.1e-10 PPR repeat family
PPR PF01535 216 - 246 2.5e-06 PPR repeat
E_motif PF20431 432 - 494 1.1e-17 E motif
Eplus_motif PF20430 499 - 522 1.5e-07 E+ motif
DYW_deaminase PF14432 530 - 621 1.1e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21065 AT4G21065
fragaria_vesca FvH4_3g09500
malus_domestica MD05G1277300.v1.1 MD10G1256200.v1.1
prunus_persica Prupe.4G087400_v2.0.a1
pyrus_communis pycom05g25840 pycom10g21200
rosa_chinensis RchiOBHm_Chr5g0015081
rosa_laevigata RLG00000032168
rosa_multiflora Rmu_co8160296.1_g000001 Rmu_sc0002209.1_g000002 Rmu_sc0009777.1_g000014 Rmu_sc0036572.1_g000001
rosa_roxburghii Rroxscaffold_1G00061450
rosa_rugosa Rorug05G0024200
rosa_samantha Rh5CG127500
rosa_wichuraiana Rw5G010250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1416
Acc65I GGTACC 1 cut(s) 229
AccB1I GGYRCC 2 cut(s) 229, 1042
AccB7I CCANNNNNTGG 1 cut(s) 1930
AccII CGCG 3 cut(s) 1338, 1568, 1570
AciI CCGC 4 cut(s) 894, 1199, 1336, 1619
AclWI GGATC 4 cut(s) 808, 821, 885, 1818
AcsI RAATTY 4 cut(s) 47, 655, 850, 1871
AcyI GRCGYC 1 cut(s) 1413
AdeI CACNNNGTG 2 cut(s) 616, 1535
AfaI GTAC 2 cut(s) 231, 1592
AfiI CCNNNNNNNGG 6 cut(s) 250, 251, 591, 1930, 1952, 1967
AflII CTTAAG 1 cut(s) 1888
AgsI TTSAA 9 cut(s) 469, 623, 832, 926, 1018, 1027, 1115, 1579, 1883
AjnI CCWGG 3 cut(s) 1348, 1705, 1985
AluBI AGCT 3 cut(s) 765, 1012, 1483
AluI AGCT 3 cut(s) 765, 1012, 1483
Alw21I GWGCWC 4 cut(s) 610, 1289, 1330, 1374
Alw26I GTCTC 3 cut(s) 283, 633, 1310
Alw44I GTGCAC 2 cut(s) 606, 1285
AlwI GGATC 4 cut(s) 808, 821, 885, 1818
AlwNI CAGNNNCTG 1 cut(s) 395
Ama87I CYCGRG 2 cut(s) 1897, 1960
AoxI GGCC 3 cut(s) 689, 1920, 1983
ApaI GGGCCC 1 cut(s) 1924
ApaLI GTGCAC 2 cut(s) 606, 1285
ApeKI GCWGC 4 cut(s) 196, 871, 1083, 1956
ApoI RAATTY 4 cut(s) 47, 655, 850, 1871
ArsI GACNNNNNNTTYG 4 cut(s) 784, 816, 835, 867
AseI ATTAAT 1 cut(s) 666
Asp700I GAANNNNTTC 1 cut(s) 1119
Asp718I GGTACC 1 cut(s) 229
AspLEI GCGC 4 cut(s) 871, 913, 1153, 1570
AspS9I GGNCC 6 cut(s) 1269, 1711, 1920, 1921, 1983, 1989
AsuC2I CCSGG 1 cut(s) 1464
AsuHPI GGTGA 6 cut(s) 128, 515, 745, 1462, 1526, 1817
AsuII TTCGAA 1 cut(s) 1123
AsuNHI GCTAGC 1 cut(s) 1555
AvaI CYCGRG 2 cut(s) 1897, 1960
AvaII GGWCC 3 cut(s) 1269, 1711, 1989
AxyI CCTNAGG 1 cut(s) 1979
BaeGI GKGCMC 4 cut(s) 610, 1047, 1289, 1924
BamHI GGATCC 1 cut(s) 813
BanI GGYRCC 2 cut(s) 229, 1042
BanII GRGCYC 2 cut(s) 1924, 1945
Bbv12I GWGCWC 4 cut(s) 610, 1289, 1330, 1374
BbvCI CCTCAGC 1 cut(s) 1484
BbvI GCAGC 4 cut(s) 208, 858, 1095, 1968
BccI CCATC 5 cut(s) 481, 740, 925, 1090, 1832
BceAI ACGGC 1 cut(s) 1620
BcgI CGANNNNNNTGC 2 cut(s) 853, 887
BciT130I CCWGG 3 cut(s) 1350, 1707, 1987
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 1 cut(s) 1464
BcoDI GTCTC 3 cut(s) 283, 633, 1310
BfaI CTAG 1 cut(s) 1556
BfmI CTRYAG 3 cut(s) 194, 1081, 2011
BfrI CTTAAG 1 cut(s) 1888
BglII AGATCT 1 cut(s) 1520
BisI GCNGC 4 cut(s) 197, 872, 1084, 1957
BlsI GCNGC 4 cut(s) 198, 873, 1085, 1958
Bme1390I CCNGG 4 cut(s) 1350, 1464, 1707, 1987
Bme18I GGWCC 3 cut(s) 1269, 1711, 1989
BmeT110I CYCGRG 2 cut(s) 1897, 1960
BmgT120I GGNCC 6 cut(s) 1269, 1711, 1920, 1921, 1983, 1989
BmiI GGNNCC 8 cut(s) 231, 815, 1044, 1712, 1713, 1922, 1944, 1976
BmrFI CCNGG 4 cut(s) 1350, 1464, 1707, 1987
BmrI ACTGGG 1 cut(s) 383
BmsI GCATC 6 cut(s) 185, 408, 430, 1341, 1400, 1883
BmtI GCTAGC 1 cut(s) 1559
BmuI ACTGGG 1 cut(s) 383
BplI GAGNNNNNCTC 2 cut(s) 1444, 1476
BpmI CTGGAG 2 cut(s) 276, 1929
Bpu10I CCTNAGC 1 cut(s) 1484
Bpu14I TTCGAA 1 cut(s) 1123
BpuEI CTTGAG 2 cut(s) 1028, 1756
BpuMI CCSGG 1 cut(s) 1464
BsaAI YACGTR 1 cut(s) 1594
BsaBI GATNNNNATC 2 cut(s) 1755, 1883
BsaHI GRCGYC 1 cut(s) 1413
BsaI GGTCTC 1 cut(s) 633
BsaJI CCNNGG 5 cut(s) 756, 1706, 1924, 1992, 2018
Bsc4I CCNNNNNNNGG 6 cut(s) 250, 251, 591, 1930, 1952, 1967
Bse1I ACTGG 3 cut(s) 293, 389, 1946
Bse21I CCTNAGG 1 cut(s) 1979
Bse3DI GCAATG 4 cut(s) 153, 173, 566, 1557
Bse8I GATNNNNATC 2 cut(s) 1755, 1883
BseBI CCWGG 3 cut(s) 1350, 1707, 1987
BseDI CCNNGG 5 cut(s) 756, 1706, 1924, 1992, 2018
BseGI GGATG 8 cut(s) 24, 176, 399, 496, 751, 1101, 1332, 1525
BseJI GATNNNNATC 2 cut(s) 1755, 1883
BseLI CCNNNNNNNGG 6 cut(s) 250, 251, 591, 1930, 1952, 1967
BseMI GCAATG 4 cut(s) 153, 173, 566, 1557
BseMII CTCAG 7 cut(s) 250, 366, 624, 774, 1041, 1475, 1970
BseNI ACTGG 3 cut(s) 293, 389, 1946
BseRI GAGGAG 5 cut(s) 94, 1364, 1643, 1646, 1649
BseSI GKGCMC 4 cut(s) 610, 1047, 1289, 1924
BseXI GCAGC 4 cut(s) 208, 858, 1095, 1968
BsgI GTGCAG 2 cut(s) 1686, 1767
Bsh1236I CGCG 3 cut(s) 1338, 1568, 1570
BshFI GGCC 3 cut(s) 691, 1922, 1985
BshNI GGYRCC 2 cut(s) 229, 1042
BsiHKAI GWGCWC 4 cut(s) 610, 1289, 1330, 1374
BsiHKCI CYCGRG 2 cut(s) 1897, 1960
BsiSI CCGG 2 cut(s) 743, 1464
BslFI GGGAC 3 cut(s) 805, 1697, 1916
BslI CCNNNNNNNGG 6 cut(s) 250, 251, 591, 1930, 1952, 1967
BsmAI GTCTC 3 cut(s) 283, 633, 1310
BsmFI GGGAC 3 cut(s) 805, 1697, 1916
BsmI GAATGC 2 cut(s) 871, 1258
BsnI GGCC 3 cut(s) 691, 1922, 1985
Bso31I GGTCTC 1 cut(s) 633
BsoBI CYCGRG 2 cut(s) 1897, 1960
Bsp119I TTCGAA 1 cut(s) 1123
Bsp120I GGGCCC 1 cut(s) 1920
Bsp1286I GDGCHC 7 cut(s) 610, 1047, 1289, 1330, 1374, 1924, 1945
Bsp143I GATC 8 cut(s) 360, 813, 877, 1053, 1520, 1563, 1789, 1810
Bsp19I CCATGG 1 cut(s) 756
BspACI CCGC 4 cut(s) 894, 1199, 1336, 1619
BspANI GGCC 3 cut(s) 691, 1922, 1985
BspCNI CTCAG 7 cut(s) 249, 367, 625, 775, 1042, 1476, 1971
BspFNI CGCG 3 cut(s) 1338, 1568, 1570
BspHI TCATGA 1 cut(s) 1875
BspLI GGNNCC 8 cut(s) 231, 815, 1044, 1712, 1713, 1922, 1944, 1976
BspMAI CTGCAG 3 cut(s) 198, 1085, 2015
BspOI GCTAGC 1 cut(s) 1559
BspPI GGATC 4 cut(s) 808, 821, 885, 1818
BspT104I TTCGAA 1 cut(s) 1123
BspT107I GGYRCC 2 cut(s) 229, 1042
BspTI CTTAAG 1 cut(s) 1888
BspTNI GGTCTC 1 cut(s) 633
BsrDI GCAATG 4 cut(s) 153, 173, 566, 1557
BsrI ACTGG 3 cut(s) 293, 389, 1946
BssECI CCNNGG 5 cut(s) 756, 1706, 1924, 1992, 2018
BssMI GATC 8 cut(s) 360, 813, 877, 1053, 1520, 1563, 1789, 1810
BssNI GRCGYC 1 cut(s) 1413
BssT1I CCWWGG 2 cut(s) 756, 1924
Bst2UI CCWGG 3 cut(s) 1350, 1707, 1987
Bst4CI ACNGT 3 cut(s) 1358, 1403, 1865
Bst6I CTCTTC 1 cut(s) 1533
BstACI GRCGYC 1 cut(s) 1413
BstAFI CTTAAG 1 cut(s) 1888
BstBAI YACGTR 1 cut(s) 1594
BstBI TTCGAA 1 cut(s) 1123
BstC8I GCNNGC 6 cut(s) 858, 1081, 1201, 1285, 1330, 1557
BstDSI CCRYGG 2 cut(s) 756, 1992
BstEII GGTNACC 1 cut(s) 134
BstF5I GGATG 8 cut(s) 24, 176, 399, 496, 751, 1101, 1332, 1525
BstFNI CGCG 3 cut(s) 1338, 1568, 1570
BstHHI GCGC 4 cut(s) 871, 913, 1153, 1570
BstKTI GATC 8 cut(s) 363, 816, 880, 1056, 1523, 1566, 1792, 1813
BstMAI GTCTC 3 cut(s) 283, 633, 1310
BstMBI GATC 8 cut(s) 360, 813, 877, 1053, 1520, 1563, 1789, 1810
BstMWI GCNNNNNNNGC 5 cut(s) 866, 1200, 1246, 1556, 1565
BstNI CCWGG 3 cut(s) 1350, 1707, 1987
BstNSI RCATGY 3 cut(s) 599, 860, 1240
BstPI GGTNACC 1 cut(s) 134
BstSCI CCNGG 4 cut(s) 1348, 1462, 1705, 1985
BstSFI CTRYAG 3 cut(s) 194, 1081, 2011
BstSLI GKGCMC 4 cut(s) 610, 1047, 1289, 1924
BstUI CGCG 3 cut(s) 1338, 1568, 1570
BstV1I GCAGC 4 cut(s) 208, 858, 1095, 1968
BstX2I RGATCY 3 cut(s) 813, 877, 1520
BstXI CCANNNNNNTGG 1 cut(s) 1993
BstYI RGATCY 3 cut(s) 813, 877, 1520
Bsu36I CCTNAGG 1 cut(s) 1979
BsuRI GGCC 3 cut(s) 691, 1922, 1985
BtgI CCRYGG 2 cut(s) 756, 1992
BtsCI GGATG 8 cut(s) 24, 176, 399, 496, 751, 1101, 1332, 1525
BtsIMutI CAGTG 3 cut(s) 1047, 1363, 1953
Cac8I GCNNGC 6 cut(s) 858, 1081, 1201, 1285, 1330, 1557
CaiI CAGNNNCTG 1 cut(s) 395
CciI TCATGA 1 cut(s) 1875
CfoI GCGC 4 cut(s) 871, 913, 1153, 1570
Cfr13I GGNCC 6 cut(s) 1269, 1711, 1920, 1921, 1983, 1989
Csp6I GTAC 2 cut(s) 230, 1591
CviQI GTAC 2 cut(s) 230, 1591
DpnI GATC 8 cut(s) 362, 815, 879, 1055, 1522, 1565, 1791, 1812
DpnII GATC 8 cut(s) 360, 813, 877, 1053, 1520, 1563, 1789, 1810
DraI TTTAAA 2 cut(s) 681, 1768
DraIII CACNNNGTG 2 cut(s) 616, 1535
Eam1104I CTCTTC 1 cut(s) 1533
EarI CTCTTC 1 cut(s) 1533
Eco130I CCWWGG 2 cut(s) 756, 1924
Eco24I GRGCYC 2 cut(s) 1924, 1945
Eco31I GGTCTC 1 cut(s) 633
Eco47I GGWCC 3 cut(s) 1269, 1711, 1989
Eco81I CCTNAGG 1 cut(s) 1979
Eco88I CYCGRG 2 cut(s) 1897, 1960
Eco91I GGTNACC 1 cut(s) 134
EcoO109I RGGNCCY 3 cut(s) 1269, 1711, 1920
EcoO65I GGTNACC 1 cut(s) 134
EcoRI GAATTC 2 cut(s) 655, 1871
EcoRII CCWGG 3 cut(s) 1348, 1705, 1985
EcoT14I CCWWGG 2 cut(s) 756, 1924
EcoT22I ATGCAT 3 cut(s) 155, 423, 1393
EcoT38I GRGCYC 2 cut(s) 1924, 1945
ErhI CCWWGG 2 cut(s) 756, 1924
FaqI GGGAC 3 cut(s) 805, 1697, 1916
FauI CCCGC 1 cut(s) 1192
FauNDI CATATG 1 cut(s) 1219
FbaI TGATCA 1 cut(s) 360
Fnu4HI GCNGC 4 cut(s) 197, 872, 1084, 1957
FokI GGATG 8 cut(s) 11, 163, 386, 503, 758, 1108, 1319, 1512
FriOI GRGCYC 2 cut(s) 1924, 1945
Fsp4HI GCNGC 4 cut(s) 197, 872, 1084, 1957
FspBI CTAG 1 cut(s) 1556
GlaI GCGC 4 cut(s) 870, 912, 1152, 1569
GluI GCNGC 4 cut(s) 197, 872, 1084, 1957
GsuI CTGGAG 2 cut(s) 276, 1929
HaeIII GGCC 3 cut(s) 691, 1922, 1985
HapII CCGG 2 cut(s) 743, 1464
HhaI GCGC 4 cut(s) 871, 913, 1153, 1570
Hin1I GRCGYC 1 cut(s) 1413
Hin6I GCGC 4 cut(s) 869, 911, 1151, 1568
HinP1I GCGC 4 cut(s) 869, 911, 1151, 1568
HinfI GANTC 3 cut(s) 542, 623, 1879
HpaII CCGG 2 cut(s) 743, 1464
HphI GGTGA 6 cut(s) 128, 515, 745, 1462, 1526, 1817
Hpy166II GTNNAC 5 cut(s) 345, 608, 753, 1287, 1506
Hpy188I TCNGA 7 cut(s) 365, 384, 634, 705, 1396, 1411, 2019
Hpy188III TCNNGA 4 cut(s) 1426, 1714, 1793, 1876
Hpy8I GTNNAC 5 cut(s) 345, 608, 753, 1287, 1506
HpyCH4III ACNGT 3 cut(s) 1358, 1403, 1865
HpyCH4IV ACGT 2 cut(s) 1413, 1593
HpyF10VI GCNNNNNNNGC 5 cut(s) 866, 1200, 1246, 1556, 1565
HpySE526I ACGT 2 cut(s) 1413, 1593
Hsp92I GRCGYC 1 cut(s) 1413
HspAI GCGC 4 cut(s) 869, 911, 1151, 1568
KflI GGGWCCC 1 cut(s) 1711
KpnI GGTACC 1 cut(s) 233
Ksp22I TGATCA 1 cut(s) 360
Kzo9I GATC 8 cut(s) 360, 813, 877, 1053, 1520, 1563, 1789, 1810
LmnI GCTCC 7 cut(s) 9, 295, 1280, 1377, 1695, 1948, 1974
Lsp1109I GCAGC 4 cut(s) 208, 858, 1095, 1968
LweI GCATC 6 cut(s) 185, 408, 430, 1341, 1400, 1883
MaeI CTAG 1 cut(s) 1556
MaeII ACGT 2 cut(s) 1413, 1593
MaeIII GTNAC 2 cut(s) 134, 1514
MalI GATC 8 cut(s) 362, 815, 879, 1055, 1522, 1565, 1791, 1812
MboI GATC 8 cut(s) 360, 813, 877, 1053, 1520, 1563, 1789, 1810
MboII GAAGA 4 cut(s) 259, 520, 1550, 1741
MfeI CAATTG 1 cut(s) 129
MflI RGATCY 3 cut(s) 813, 877, 1520
MhlI GDGCHC 7 cut(s) 610, 1047, 1289, 1330, 1374, 1924, 1945
MluCI AATT 7 cut(s) 47, 129, 655, 850, 1146, 1489, 1871
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 2 cut(s) 427, 475
Mph1103I ATGCAT 3 cut(s) 155, 423, 1393
MroXI GAANNNNTTC 1 cut(s) 1119
MseI TTAA 7 cut(s) 62, 558, 666, 680, 984, 1767, 1889
MslI CAYNNNNRTG 2 cut(s) 579, 1241
MspCI CTTAAG 1 cut(s) 1888
MspI CCGG 2 cut(s) 743, 1464
MspR9I CCNGG 4 cut(s) 1350, 1464, 1707, 1987
MunI CAATTG 1 cut(s) 129
Mva1269I GAATGC 2 cut(s) 871, 1258
MvaI CCWGG 3 cut(s) 1350, 1707, 1987
MvnI CGCG 3 cut(s) 1338, 1568, 1570
MwoI GCNNNNNNNGC 5 cut(s) 866, 1200, 1246, 1556, 1565
NciI CCSGG 1 cut(s) 1464
NcoI CCATGG 1 cut(s) 756
NdeI CATATG 1 cut(s) 1219
NdeII GATC 8 cut(s) 360, 813, 877, 1053, 1520, 1563, 1789, 1810
NheI GCTAGC 1 cut(s) 1555
NlaIV GGNNCC 8 cut(s) 231, 815, 1044, 1712, 1713, 1922, 1944, 1976
NmeAIII GCCGAG 1 cut(s) 1179
NmuCI GTSAC 2 cut(s) 134, 1514
NsiI ATGCAT 3 cut(s) 155, 423, 1393
NspI RCATGY 3 cut(s) 599, 860, 1240
NspV TTCGAA 1 cut(s) 1123
PaeI GCATGC 1 cut(s) 860
PagI TCATGA 1 cut(s) 1875
PcsI WCGNNNNNNNCGW 1 cut(s) 860
PctI GAATGC 2 cut(s) 871, 1258
PdmI GAANNNNTTC 1 cut(s) 1119
PfeI GAWTC 2 cut(s) 623, 1879
PflFI GACNNNGTC 1 cut(s) 644
PflMI CCANNNNNTGG 1 cut(s) 1930
PfoI TCCNGGA 1 cut(s) 1462
PkrI GCNGC 4 cut(s) 198, 873, 1085, 1958
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
Ppu21I YACGTR 1 cut(s) 1594
PpuMI RGGWCCY 2 cut(s) 1269, 1711
PshBI ATTAAT 1 cut(s) 666
Psp5II RGGWCCY 2 cut(s) 1269, 1711
Psp6I CCWGG 3 cut(s) 1348, 1705, 1985
PspEI GGTNACC 1 cut(s) 134
PspGI CCWGG 3 cut(s) 1348, 1705, 1985
PspN4I GGNNCC 8 cut(s) 231, 815, 1044, 1712, 1713, 1922, 1944, 1976
PspOMI GGGCCC 1 cut(s) 1920
PspPI GGNCC 6 cut(s) 1269, 1711, 1920, 1921, 1983, 1989
PspPPI RGGWCCY 2 cut(s) 1269, 1711
PstI CTGCAG 3 cut(s) 198, 1085, 2015
PstNI CAGNNNCTG 1 cut(s) 395
PsuI RGATCY 3 cut(s) 813, 877, 1520
PsyI GACNNNGTC 1 cut(s) 644
RsaI GTAC 2 cut(s) 231, 1592
RsaNI GTAC 2 cut(s) 230, 1591
RseI CAYNNNNRTG 2 cut(s) 579, 1241
SaqAI TTAA 7 cut(s) 62, 558, 666, 680, 984, 1767, 1889
SatI GCNGC 4 cut(s) 197, 872, 1084, 1957
Sau3AI GATC 8 cut(s) 360, 813, 877, 1053, 1520, 1563, 1789, 1810
Sau96I GGNCC 6 cut(s) 1269, 1711, 1920, 1921, 1983, 1989
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 4 cut(s) 1350, 1464, 1707, 1987
SduI GDGCHC 7 cut(s) 610, 1047, 1289, 1330, 1374, 1924, 1945
SfaNI GCATC 6 cut(s) 185, 408, 430, 1341, 1400, 1883
SfcI CTRYAG 3 cut(s) 194, 1081, 2011
SfuI TTCGAA 1 cut(s) 1123
SinI GGWCC 3 cut(s) 1269, 1711, 1989
SmiMI CAYNNNNRTG 2 cut(s) 579, 1241
SmlI CTYRAG 3 cut(s) 1007, 1735, 1888
SmoI CTYRAG 3 cut(s) 1007, 1735, 1888
SphI GCATGC 1 cut(s) 860
Sse9I AATT 7 cut(s) 47, 129, 655, 850, 1146, 1489, 1871
SsiI CCGC 4 cut(s) 894, 1199, 1336, 1619
SspI AATATT 1 cut(s) 315
SspMI CTAG 1 cut(s) 1556
StyD4I CCNGG 4 cut(s) 1348, 1462, 1705, 1985
StyI CCWWGG 2 cut(s) 756, 1924
TaaI ACNGT 3 cut(s) 1358, 1403, 1865
TaiI ACGT 2 cut(s) 1416, 1596
TaqI TCGA 2 cut(s) 1123, 1778
TasI AATT 7 cut(s) 47, 129, 655, 850, 1146, 1489, 1871
TfiI GAWTC 2 cut(s) 623, 1879
Tru1I TTAA 7 cut(s) 62, 558, 666, 680, 984, 1767, 1889
Tru9I TTAA 7 cut(s) 62, 558, 666, 680, 984, 1767, 1889
TscAI CASTG 3 cut(s) 1054, 1363, 1953
TseFI GTSAC 2 cut(s) 134, 1514
TseI GCWGC 4 cut(s) 196, 871, 1083, 1956
Tsp45I GTSAC 2 cut(s) 134, 1514
TspDTI ATGAA 7 cut(s) 563, 1119, 1145, 1236, 1247, 1259, 1864
TspGWI ACGGA 2 cut(s) 421, 1981
TspRI CASTG 3 cut(s) 1054, 1363, 1953
Tth111I GACNNNGTC 1 cut(s) 644
Van91I CCANNNNNTGG 1 cut(s) 1930
Vha464I CTTAAG 1 cut(s) 1888
VneI GTGCAC 2 cut(s) 606, 1285
VpaK11BI GGWCC 3 cut(s) 1269, 1711, 1989
VspI ATTAAT 1 cut(s) 666
XapI RAATTY 4 cut(s) 47, 655, 850, 1871
XceI RCATGY 3 cut(s) 599, 860, 1240
XcmI CCANNNNNNNNNTGG 1 cut(s) 1835
XmnI GAANNNNTTC 1 cut(s) 1119
XspI CTAG 1 cut(s) 1556
ZraI GACGTC 1 cut(s) 1414
Zsp2I ATGCAT 3 cut(s) 155, 423, 1393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.