MD10G1256200.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0013500
Physical Location & Seq
Reverse (-)
35058752 .. 35060770
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1256200.v1.1.491

Sequence Viewer

Length: 858 bp
ATGCTCCCAAAAGAAGCAGGCTCAGTAAAAGAAGCATACGAATACATTATGAATATGCCTGTGCAGCCAAATGCTGTTATTTGGAGGACCTTATTAGGAGCGTGCACGATACACGGGCAGCTCGCCCTTGGAGAGACTGCAAGAGCGCACATTCGAGAGTTAGAGCCCGGACATAGTGGGGATTATGTGCTCCTATCCAATCTCTACGCATCTGAACGCCGTTGGTTAGATGTTCAAAAGGTGAGGAGGACAATGTTTAGTGAAGGGGTGAGGAAAACTCCCGGGTACAGTATTGTTGAGCTGAGGAATTGTATTTGTGAGTTCACTATGGGTGACAGATCTCATCCACAAAGTGAAGAGATATACGCAATGCTAGCAGATATCACACGATTGTTGAAACTGGAAGGGTATGTGCCTCATACAACAAATGTTCTTGCAGACATAGAGGAGGAGGAAAAGGAAAATGCTTTGTCCTATCATAGTGAAAATATCGCAATTGCATTTATGCTCCTTAATACTGCACCAGGGATGCCGATTAGGATTTGGAAGAATTTGAGAGTTTGCGCAGATTGTCATCTGGCAATTAAACTCGTATCGAAGGTTTATGACCGGGAGATTGTTGTGAGGGATCGTAGTCGGTTTCACCATTTTAGAGATGGTTCTTGTTCTTGTAGATATTACTGCTTTAGGATGGAAGGTGGTCCGGATGCCGGATCTAAGAATTTCTCCCAGTTTAGCCCTGGTGTACAATTTAATGGATATTCGAAAAAGACGAAAAAGGAAAGACAAAAGAAGAAGGGAGGAAATAGTGCATTCGAATCCGTTAATCCAAACAAGCCCTTAAGTCGAAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2 X8

Protein Analysis

286

Amino Acids

32.5

Weight (kDa)

9.02

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 38 - 100 1.6e-18 E motif
Eplus_motif PF20430 107 - 130 6.9e-08 E+ motif
DYW_deaminase PF14432 136 - 226 6.3e-38 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013500)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G21065 AT4G21065
fragaria_vesca FvH4_3g09500
malus_domestica MD05G1277300.v1.1 MD10G1256200.v1.1
prunus_persica Prupe.4G087400_v2.0.a1
pyrus_communis pycom05g25840 pycom10g21200
rosa_chinensis RchiOBHm_Chr5g0015081
rosa_laevigata RLG00000032168
rosa_multiflora Rmu_co8160296.1_g000001 Rmu_sc0002209.1_g000002 Rmu_sc0009777.1_g000014 Rmu_sc0036572.1_g000001
rosa_roxburghii Rroxscaffold_1G00061450
rosa_rugosa Rorug05G0024200
rosa_samantha Rh5CG127500
rosa_wichuraiana Rw5G010250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 565
AccIII TCCGGA 1 cut(s) 703
AclWI GGATC 2 cut(s) 636, 721
AcsI RAATTY 3 cut(s) 550, 721, 851
AdeI CACNNNGTG 1 cut(s) 353
AfaI GTAC 2 cut(s) 287, 747
AfiI CCNNNNNNNGG 1 cut(s) 710
AflII CTTAAG 1 cut(s) 841
AgsI TTSAA 2 cut(s) 236, 397
AjnI CCWGG 2 cut(s) 523, 739
AluBI AGCT 2 cut(s) 121, 301
AluI AGCT 2 cut(s) 121, 301
Alw21I GWGCWC 2 cut(s) 107, 192
Alw26I GTCTC 1 cut(s) 128
Alw44I GTGCAC 1 cut(s) 103
AlwI GGATC 2 cut(s) 636, 721
Ama87I CYCGRG 1 cut(s) 281
Aor13HI TCCGGA 1 cut(s) 703
ApaLI GTGCAC 1 cut(s) 103
ApeKI GCWGC 2 cut(s) 64, 118
ApoI RAATTY 3 cut(s) 550, 721, 851
AspLEI GCGC 2 cut(s) 148, 566
AspS9I GGNCC 2 cut(s) 87, 701
AsuC2I CCSGG 4 cut(s) 168, 282, 283, 611
AsuHPI GGTGA 4 cut(s) 253, 280, 344, 635
AsuII TTCGAA 2 cut(s) 764, 816
AsuNHI GCTAGC 1 cut(s) 373
AvaI CYCGRG 1 cut(s) 281
AvaII GGWCC 2 cut(s) 87, 701
BaeGI GKGCMC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 2 cut(s) 107, 192
BbvCI CCTCAGC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 76, 130
BccI CCATC 2 cut(s) 650, 685
BceAI ACGGC 1 cut(s) 204
BciT130I CCWGG 2 cut(s) 525, 741
BcnI CCSGG 4 cut(s) 168, 282, 283, 611
BcoDI GTCTC 1 cut(s) 128
BfaI CTAG 1 cut(s) 374
BfrI CTTAAG 1 cut(s) 841
BglII AGATCT 1 cut(s) 338
BisI GCNGC 2 cut(s) 65, 119
BlsI GCNGC 2 cut(s) 66, 120
Bme1390I CCNGG 6 cut(s) 168, 282, 283, 525, 611, 741
Bme18I GGWCC 2 cut(s) 87, 701
BmeT110I CYCGRG 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 87, 701
BmrFI CCNGG 6 cut(s) 168, 282, 283, 525, 611, 741
BmrI ACTGGG 1 cut(s) 724
BmsI GCATC 3 cut(s) 218, 519, 697
BmtI GCTAGC 1 cut(s) 377
BmuI ACTGGG 1 cut(s) 724
BplI GAGNNNNNCTC 2 cut(s) 262, 294
Bpu10I CCTNAGC 1 cut(s) 302
Bpu14I TTCGAA 2 cut(s) 764, 816
BpuMI CCSGG 4 cut(s) 168, 282, 283, 611
BsaBI GATNNNNATC 1 cut(s) 573
BsaJI CCNNGG 4 cut(s) 127, 281, 524, 739
BsaWI WCCGGW 1 cut(s) 703
Bsc4I CCNNNNNNNGG 1 cut(s) 710
Bse1I ACTGG 2 cut(s) 405, 730
Bse3DI GCAATG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 573
BseAI TCCGGA 1 cut(s) 703
BseBI CCWGG 2 cut(s) 525, 741
BseDI CCNNGG 4 cut(s) 127, 281, 524, 739
BseGI GGATG 4 cut(s) 343, 534, 696, 712
BseJI GATNNNNATC 1 cut(s) 573
BseLI CCNNNNNNNGG 1 cut(s) 710
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 36, 293
BseNI ACTGG 2 cut(s) 405, 730
BseRI GAGGAG 3 cut(s) 259, 461, 464
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 2 cut(s) 76, 130
BsgI GTGCAG 2 cut(s) 83, 504
BsiHKAI GWGCWC 2 cut(s) 107, 192
BsiHKCI CYCGRG 1 cut(s) 281
BsiSI CCGG 5 cut(s) 168, 282, 610, 704, 711
BslI CCNNNNNNNGG 1 cut(s) 710
BsmAI GTCTC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 812
BsoBI CYCGRG 1 cut(s) 281
Bsp119I TTCGAA 2 cut(s) 764, 816
Bsp1286I GDGCHC 3 cut(s) 107, 168, 192
Bsp13I TCCGGA 1 cut(s) 703
Bsp1407I TGTACA 1 cut(s) 745
Bsp143I GATC 3 cut(s) 338, 628, 713
BspCNI CTCAG 2 cut(s) 35, 294
BspEI TCCGGA 1 cut(s) 703
BspOI GCTAGC 1 cut(s) 377
BspPI GGATC 2 cut(s) 636, 721
BspT104I TTCGAA 2 cut(s) 764, 816
BspTI CTTAAG 1 cut(s) 841
BsrDI GCAATG 1 cut(s) 375
BsrGI TGTACA 1 cut(s) 745
BsrI ACTGG 2 cut(s) 405, 730
BssECI CCNNGG 4 cut(s) 127, 281, 524, 739
BssMI GATC 3 cut(s) 338, 628, 713
BssT1I CCWWGG 1 cut(s) 127
Bst2UI CCWGG 2 cut(s) 525, 741
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 351
BstAFI CTTAAG 1 cut(s) 841
BstAUI TGTACA 1 cut(s) 745
BstBI TTCGAA 2 cut(s) 764, 816
BstC8I GCNNGC 4 cut(s) 19, 103, 123, 375
BstDEI CTNAG 3 cut(s) 22, 302, 717
BstF5I GGATG 4 cut(s) 343, 534, 696, 712
BstHHI GCGC 2 cut(s) 148, 566
BstKTI GATC 3 cut(s) 341, 631, 716
BstMAI GTCTC 1 cut(s) 128
BstMBI GATC 3 cut(s) 338, 628, 713
BstMWI GCNNNNNNNGC 2 cut(s) 64, 374
BstNI CCWGG 2 cut(s) 525, 741
BstSCI CCNGG 6 cut(s) 166, 280, 281, 523, 609, 739
BstSLI GKGCMC 1 cut(s) 107
BstV1I GCAGC 2 cut(s) 76, 130
BstX2I RGATCY 2 cut(s) 338, 713
BstYI RGATCY 2 cut(s) 338, 713
BtsCI GGATG 4 cut(s) 343, 534, 696, 712
Cac8I GCNNGC 4 cut(s) 19, 103, 123, 375
CfoI GCGC 2 cut(s) 148, 566
Cfr13I GGNCC 2 cut(s) 87, 701
Cfr9I CCCGGG 1 cut(s) 281
Csp6I GTAC 2 cut(s) 286, 746
CviJI RGCY 7 cut(s) 21, 67, 121, 166, 301, 738, 838
CviKI_1 RGCY 7 cut(s) 21, 67, 121, 166, 301, 738, 838
CviQI GTAC 2 cut(s) 286, 746
DdeI CTNAG 3 cut(s) 22, 302, 717
DpnI GATC 3 cut(s) 340, 630, 715
DpnII GATC 3 cut(s) 338, 628, 713
DraIII CACNNNGTG 1 cut(s) 353
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 127
Eco24I GRGCYC 1 cut(s) 168
Eco32I GATATC 1 cut(s) 382
Eco47I GGWCC 2 cut(s) 87, 701
Eco88I CYCGRG 1 cut(s) 281
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 523, 739
EcoRV GATATC 1 cut(s) 382
EcoT14I CCWWGG 1 cut(s) 127
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 127
Fnu4HI GCNGC 2 cut(s) 65, 119
FokI GGATG 4 cut(s) 330, 541, 703, 719
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 2 cut(s) 65, 119
FspBI CTAG 1 cut(s) 374
FspI TGCGCA 1 cut(s) 565
GlaI GCGC 2 cut(s) 147, 565
GluI GCNGC 2 cut(s) 65, 119
HapII CCGG 5 cut(s) 168, 282, 610, 704, 711
HhaI GCGC 2 cut(s) 148, 566
Hin6I GCGC 2 cut(s) 146, 564
HinP1I GCGC 2 cut(s) 146, 564
HinfI GANTC 1 cut(s) 818
HpaII CCGG 5 cut(s) 168, 282, 610, 704, 711
HphI GGTGA 4 cut(s) 253, 280, 344, 635
Hpy166II GTNNAC 3 cut(s) 105, 324, 746
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 2 cut(s) 155, 704
Hpy8I GTNNAC 3 cut(s) 105, 324, 746
HpyAV CCTTC 5 cut(s) 257, 398, 592, 689, 790
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4V TGCA 7 cut(s) 64, 105, 140, 437, 500, 521, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 64, 374
HpyF3I CTNAG 3 cut(s) 22, 302, 717
HspAI GCGC 2 cut(s) 146, 564
Kpn2I TCCGGA 1 cut(s) 703
Kzo9I GATC 3 cut(s) 338, 628, 713
LmnI GCTCC 4 cut(s) 9, 98, 195, 513
Lsp1109I GCAGC 2 cut(s) 76, 130
LweI GCATC 3 cut(s) 218, 519, 697
MaeI CTAG 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 332
MalI GATC 3 cut(s) 340, 630, 715
MboI GATC 3 cut(s) 338, 628, 713
MboII GAAGA 3 cut(s) 368, 559, 805
MfeI CAATTG 1 cut(s) 495
MflI RGATCY 2 cut(s) 338, 713
MhlI GDGCHC 3 cut(s) 107, 168, 192
MluCI AATT 7 cut(s) 307, 495, 550, 582, 721, 749, 851
MroI TCCGGA 1 cut(s) 703
MseI TTAA 5 cut(s) 513, 585, 753, 825, 842
MspCI CTTAAG 1 cut(s) 841
MspI CCGG 5 cut(s) 168, 282, 610, 704, 711
MspR9I CCNGG 6 cut(s) 168, 282, 283, 525, 611, 741
MunI CAATTG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 812
MvaI CCWGG 2 cut(s) 525, 741
MwoI GCNNNNNNNGC 2 cut(s) 64, 374
NciI CCSGG 4 cut(s) 168, 282, 283, 611
NdeII GATC 3 cut(s) 338, 628, 713
NheI GCTAGC 1 cut(s) 373
NmuCI GTSAC 1 cut(s) 332
NsbI TGCGCA 1 cut(s) 565
NspV TTCGAA 2 cut(s) 764, 816
PcsI WCGNNNNNNNCGW 1 cut(s) 770
PctI GAATGC 1 cut(s) 812
PfeI GAWTC 1 cut(s) 818
PkrI GCNGC 2 cut(s) 66, 120
PpuMI RGGWCCY 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 523, 739
PspGI CCWGG 2 cut(s) 523, 739
PspPI GGNCC 2 cut(s) 87, 701
PspPPI RGGWCCY 1 cut(s) 87
PsuI RGATCY 2 cut(s) 338, 713
RsaI GTAC 2 cut(s) 287, 747
RsaNI GTAC 2 cut(s) 286, 746
SaqAI TTAA 5 cut(s) 513, 585, 753, 825, 842
SatI GCNGC 2 cut(s) 65, 119
Sau3AI GATC 3 cut(s) 338, 628, 713
Sau96I GGNCC 2 cut(s) 87, 701
ScrFI CCNGG 6 cut(s) 168, 282, 283, 525, 611, 741
SduI GDGCHC 3 cut(s) 107, 168, 192
SetI ASST 6 cut(s) 92, 123, 243, 303, 603, 700
SfaNI GCATC 3 cut(s) 218, 519, 697
SfuI TTCGAA 2 cut(s) 764, 816
SinI GGWCC 2 cut(s) 87, 701
SmaI CCCGGG 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 841
SmoI CTYRAG 1 cut(s) 841
Sse9I AATT 7 cut(s) 307, 495, 550, 582, 721, 749, 851
SspMI CTAG 1 cut(s) 374
StyD4I CCNGG 6 cut(s) 166, 280, 281, 523, 609, 739
StyI CCWWGG 1 cut(s) 127
TaaI ACNGT 1 cut(s) 290
TaqI TCGA 5 cut(s) 154, 596, 764, 816, 847
TasI AATT 7 cut(s) 307, 495, 550, 582, 721, 749, 851
TatI WGTACW 1 cut(s) 745
TfiI GAWTC 1 cut(s) 818
Tru1I TTAA 5 cut(s) 513, 585, 753, 825, 842
Tru9I TTAA 5 cut(s) 513, 585, 753, 825, 842
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 64, 118
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 65
TspGWI ACGGA 1 cut(s) 811
TspMI CCCGGG 1 cut(s) 281
Vha464I CTTAAG 1 cut(s) 841
VneI GTGCAC 1 cut(s) 103
VpaK11BI GGWCC 2 cut(s) 87, 701
XapI RAATTY 3 cut(s) 550, 721, 851
XcmI CCANNNNNNNNNTGG 2 cut(s) 653, 737
XmaI CCCGGG 1 cut(s) 281
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.