AT4G23550
WRKY Family

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011031
Physical Location & Seq
Forward (+)
12291632 .. 12293367
1736 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G23550.1

Sequence Viewer

Length: 933 bp
ATGGGTGAGGTGGCTTATATGGACGAAGGAGACCTAGAAGCAATAGTCAGAGGCTACTCCGGCTCCGGAGACGCGTTTTCCGGCGAAAGTTCCGGTACGTTTTCACCTTCGTTTTGCCTACCGATGGAGACGTCTAGTTTCTACGAACCGGAGATGGAGACAAGTGGCTTAGATGAGCTCGGTGAACTTTACAAACCCTTTTACCCTTTCTCCACACAAACGATCCTCACAAGCTCGGTCTCTCTCCCTGAAGATTCAAAACCTTTCCGAGATGACAAGAAACAACGATCACATGGTTGTCTTTTATCCAACGGATCAAGAGCTGATCATATCCGAATTTCAGAATCCAAATCAAAGAAAAGCAAGAAGAATCAACAGAAGAGAGTTGTTGAGCAAGTGAAAGAAGAGAATCTGTTGTCGGACGCATGGGCGTGGCGTAAATACGGGCAGAAACCCATCAAAGGATCTCCATACCCAAGGAGTTATTACAGATGCAGTAGCTCAAAAGGGTGTTTGGCAAGAAAACAAGTCGAAAGAAATCCTCAAAACCCGGAGAAATTCACCATAACATACACTAATGAGCACAATCATGAACTACCAACCCGGAGAAACTCATTAGCCGGTTCGACTCGAGCAAAAACTTCCCAACCCAAACCAACCTTAACCAAAAAATCCGAAAAAGAAGTTGTTTCTTCCCCTACAAGTAATCCTATGATCCCATCCGCTGATGAATCTTCTGTTGCGGTTCAAGAAATGAGCGTTGCGGAAACGAGTACGCACCAAGCGGCTGGAGCAATCGAGGGCCGCCGCTTGAGTAACGGTTTACCATCGGATTTGATGTCCGGGAGCGGAACTTTTCCAAGTTTTACCGGTGACTTCGATGAACTATTGAATAGCCAAGAGTTCTTCAGTGGGTATTTATGGAATTACTAG

Protein Analysis

310

Amino Acids

34.42

Weight (kDa)

6.27

Isoelectric Point (pI)

53.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 141 - 198 2.3e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23550
fragaria_vesca FvH4_5g03210 FvH4_5g03210
malus_domestica MD06G1091200.v1.1 MD14G1112200.v1.1
prunus_persica Prupe.5G106700_v2.0.a1 Prupe.5G106700_v2.0.a1
pyrus_communis pycom06g08670 pycom14g10690
rosa_chinensis RchiOBHm_Chr7g0196571
rosa_laevigata RLG00000004005
rosa_multiflora Rmu_sc0006080.1_g000001
rosa_roxburghii Rroxscaffold_3G00259200
rosa_rugosa Rorug07G0034700 Rorug07G0034800
rosa_samantha Rh7AG158800 Rh7BG160800 Rh7CG167200 Rh7DG160000
rosa_wichuraiana Rw7G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 134
AccBSI CCGCTC 1 cut(s) 849
AccII CGCG 1 cut(s) 74
AccIII TCCGGA 1 cut(s) 65
AciI CCGC 7 cut(s) 723, 743, 764, 785, 805, 808, 849
AclWI GGATC 4 cut(s) 217, 322, 472, 709
AcsI RAATTY 2 cut(s) 336, 557
AcuI CTGAAG 2 cut(s) 270, 892
AcyI GRCGYC 1 cut(s) 131
AfaI GTAC 2 cut(s) 97, 775
AfiI CCNNNNNNNGG 2 cut(s) 124, 461
AflIII ACRYGT 1 cut(s) 72
AgeI ACCGGT 1 cut(s) 869
AgsI TTSAA 3 cut(s) 258, 749, 892
AluBI AGCT 4 cut(s) 178, 234, 323, 501
AluI AGCT 4 cut(s) 178, 234, 323, 501
Alw21I GWGCWC 2 cut(s) 180, 585
Alw26I GTCTC 5 cut(s) 24, 63, 122, 152, 244
AlwI GGATC 4 cut(s) 217, 322, 472, 709
Ama87I CYCGRG 1 cut(s) 630
Aor13HI TCCGGA 1 cut(s) 65
AoxI GGCC 1 cut(s) 802
ApoI RAATTY 2 cut(s) 336, 557
AsiGI ACCGGT 1 cut(s) 869
AspS9I GGNCC 1 cut(s) 802
AsuC2I CCSGG 3 cut(s) 551, 604, 844
AsuHPI GGTGA 5 cut(s) 17, 96, 194, 553, 884
AvaI CYCGRG 1 cut(s) 630
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 180, 585
BccI CCATC 5 cut(s) 118, 148, 464, 727, 835
BclI TGATCA 1 cut(s) 325
BcnI CCSGG 3 cut(s) 551, 604, 844
BcoDI GTCTC 5 cut(s) 24, 63, 122, 152, 244
BfaI CTAG 3 cut(s) 35, 135, 931
BisI GCNGC 3 cut(s) 786, 805, 808
BlsI GCNGC 3 cut(s) 787, 806, 809
Bme1390I CCNGG 3 cut(s) 551, 604, 844
BmeT110I CYCGRG 1 cut(s) 630
BmgT120I GGNCC 1 cut(s) 802
BmiI GGNNCC 1 cut(s) 64
BmrFI CCNGG 3 cut(s) 551, 604, 844
BmsI GCATC 1 cut(s) 482
BpmI CTGGAG 1 cut(s) 810
BpuEI CTTGAG 1 cut(s) 832
BpuMI CCSGG 3 cut(s) 551, 604, 844
BsaHI GRCGYC 1 cut(s) 131
BsaI GGTCTC 2 cut(s) 24, 244
BsaJI CCNNGG 1 cut(s) 476
BsaWI WCCGGW 4 cut(s) 65, 92, 148, 869
BsaXI ACNNNNNCTCC 4 cut(s) 47, 77, 194, 224
Bsc4I CCNNNNNNNGG 2 cut(s) 124, 461
Bse118I RCCGGY 2 cut(s) 620, 869
BseAI TCCGGA 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 476
BseGI GGATG 1 cut(s) 719
BseLI CCNNNNNNNGG 2 cut(s) 124, 461
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 1 cut(s) 804
BshTI ACCGGT 1 cut(s) 869
BsiHKAI GWGCWC 2 cut(s) 180, 585
BsiHKCI CYCGRG 1 cut(s) 630
BslI CCNNNNNNNGG 2 cut(s) 124, 461
BsmAI GTCTC 5 cut(s) 24, 63, 122, 152, 244
BsmBI CGTCTC 2 cut(s) 63, 122
BsnI GGCC 1 cut(s) 804
Bso31I GGTCTC 2 cut(s) 24, 244
BsoBI CYCGRG 1 cut(s) 630
Bsp1286I GDGCHC 2 cut(s) 180, 585
Bsp13I TCCGGA 1 cut(s) 65
Bsp143I GATC 6 cut(s) 222, 287, 314, 325, 464, 714
BspACI CCGC 7 cut(s) 723, 743, 764, 785, 805, 808, 849
BspANI GGCC 1 cut(s) 804
BspEI TCCGGA 1 cut(s) 65
BspFNI CGCG 1 cut(s) 74
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 1 cut(s) 64
BspPI GGATC 4 cut(s) 217, 322, 472, 709
BspTNI GGTCTC 2 cut(s) 24, 244
BsrBI CCGCTC 1 cut(s) 849
BsrFI RCCGGY 2 cut(s) 620, 869
BssAI RCCGGY 2 cut(s) 620, 869
BssECI CCNNGG 1 cut(s) 476
BssMI GATC 6 cut(s) 222, 287, 314, 325, 464, 714
BssNI GRCGYC 1 cut(s) 131
BssT1I CCWWGG 1 cut(s) 476
Bst4CI ACNGT 1 cut(s) 821
Bst6I CTCTTC 2 cut(s) 374, 399
BstACI GRCGYC 1 cut(s) 131
BstDEI CTNAG 1 cut(s) 169
BstF5I GGATG 1 cut(s) 719
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 6 cut(s) 225, 290, 317, 328, 467, 717
BstMAI GTCTC 5 cut(s) 24, 63, 122, 152, 244
BstMBI GATC 6 cut(s) 222, 287, 314, 325, 464, 714
BstMWI GCNNNNNNNGC 2 cut(s) 60, 791
BstSCI CCNGG 3 cut(s) 549, 602, 842
BstUI CGCG 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 464
BstXI CCANNNNNNTGG 1 cut(s) 788
BstYI RGATCY 1 cut(s) 464
BsuRI GGCC 1 cut(s) 804
BtsCI GGATG 1 cut(s) 719
BtsIMutI CAGTG 1 cut(s) 916
CciI TCATGA 1 cut(s) 589
Cfr10I RCCGGY 2 cut(s) 620, 869
Cfr13I GGNCC 1 cut(s) 802
CseI GACGC 2 cut(s) 80, 431
Csp6I GTAC 2 cut(s) 96, 774
CspAI ACCGGT 1 cut(s) 869
CviAII CATG 3 cut(s) 293, 426, 590
CviQI GTAC 2 cut(s) 96, 774
DdeI CTNAG 1 cut(s) 169
DpnI GATC 6 cut(s) 224, 289, 316, 327, 466, 716
DpnII GATC 6 cut(s) 222, 287, 314, 325, 464, 714
Eam1104I CTCTTC 2 cut(s) 374, 399
EarI CTCTTC 2 cut(s) 374, 399
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 180
Eco31I GGTCTC 2 cut(s) 24, 244
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 2 cut(s) 270, 892
Eco88I CYCGRG 1 cut(s) 630
EcoICRI GAGCTC 1 cut(s) 178
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 476
Esp3I CGTCTC 2 cut(s) 63, 122
FaeI CATG 3 cut(s) 296, 429, 593
FatI CATG 3 cut(s) 292, 425, 589
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 3 cut(s) 786, 805, 808
FokI GGATG 1 cut(s) 706
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 3 cut(s) 786, 805, 808
FspBI CTAG 3 cut(s) 35, 135, 931
GluI GCNGC 3 cut(s) 786, 805, 808
GsuI CTGGAG 1 cut(s) 810
HaeIII GGCC 1 cut(s) 804
HgaI GACGC 2 cut(s) 80, 431
Hin1I GRCGYC 1 cut(s) 131
Hin1II CATG 3 cut(s) 296, 429, 593
HinfI GANTC 6 cut(s) 254, 344, 370, 409, 628, 731
HphI GGTGA 5 cut(s) 17, 96, 194, 553, 884
Hpy166II GTNNAC 2 cut(s) 185, 824
Hpy188I TCNGA 7 cut(s) 50, 269, 335, 343, 421, 676, 832
Hpy188III TCNNGA 4 cut(s) 66, 318, 590, 749
Hpy8I GTNNAC 2 cut(s) 185, 824
HpyAV CCTTC 2 cut(s) 20, 117
HpyCH4III ACNGT 1 cut(s) 821
HpyCH4IV ACGT 2 cut(s) 98, 131
HpyCH4V TGCA 1 cut(s) 495
HpyF10VI GCNNNNNNNGC 2 cut(s) 60, 791
HpyF3I CTNAG 1 cut(s) 169
HpySE526I ACGT 2 cut(s) 98, 131
Hsp92I GRCGYC 1 cut(s) 131
Hsp92II CATG 3 cut(s) 296, 429, 593
Kpn2I TCCGGA 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 6 cut(s) 222, 287, 314, 325, 464, 714
LmnI GCTCC 3 cut(s) 68, 791, 846
LweI GCATC 1 cut(s) 482
MaeI CTAG 3 cut(s) 35, 135, 931
MaeII ACGT 2 cut(s) 98, 131
MaeIII GTNAC 2 cut(s) 815, 872
MalI GATC 6 cut(s) 224, 289, 316, 327, 466, 716
MbiI CCGCTC 1 cut(s) 849
MboI GATC 6 cut(s) 222, 287, 314, 325, 464, 714
MboII GAAGA 7 cut(s) 263, 379, 391, 416, 684, 726, 898
MflI RGATCY 1 cut(s) 464
MhlI GDGCHC 2 cut(s) 180, 585
MluCI AATT 3 cut(s) 336, 557, 925
MluI ACGCGT 1 cut(s) 72
MlyI GAGTC 1 cut(s) 622
MmeI TCCRAC 2 cut(s) 333, 399
MnlI CCTC 4 cut(s) 44, 236, 552, 793
MroI TCCGGA 1 cut(s) 65
MseI TTAA 1 cut(s) 662
MslI CAYNNNNRTG 2 cut(s) 430, 588
MspA1I CMGCKG 1 cut(s) 725
MspR9I CCNGG 3 cut(s) 551, 604, 844
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 2 cut(s) 60, 791
NciI CCSGG 3 cut(s) 551, 604, 844
NdeII GATC 6 cut(s) 222, 287, 314, 325, 464, 714
NlaIII CATG 3 cut(s) 296, 429, 593
NlaIV GGNNCC 1 cut(s) 64
NmuCI GTSAC 1 cut(s) 872
PaeR7I CTCGAG 1 cut(s) 630
PagI TCATGA 1 cut(s) 589
PfeI GAWTC 5 cut(s) 254, 344, 370, 409, 731
PfoI TCCNGGA 1 cut(s) 842
PinAI ACCGGT 1 cut(s) 869
PkrI GCNGC 3 cut(s) 787, 806, 809
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
Psp124BI GAGCTC 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 1 cut(s) 802
PspXI VCTCGAGB 1 cut(s) 630
PsuI RGATCY 1 cut(s) 464
RsaI GTAC 2 cut(s) 97, 775
RsaNI GTAC 2 cut(s) 96, 774
RseI CAYNNNNRTG 2 cut(s) 430, 588
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 1 cut(s) 662
SatI GCNGC 3 cut(s) 786, 805, 808
Sau3AI GATC 6 cut(s) 222, 287, 314, 325, 464, 714
Sau96I GGNCC 1 cut(s) 802
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 3 cut(s) 551, 604, 844
SduI GDGCHC 2 cut(s) 180, 585
SfaNI GCATC 1 cut(s) 482
Sfr274I CTCGAG 1 cut(s) 630
SlaI CTCGAG 1 cut(s) 630
SmiMI CAYNNNNRTG 2 cut(s) 430, 588
SmlI CTYRAG 2 cut(s) 630, 811
SmoI CTYRAG 2 cut(s) 630, 811
Sse9I AATT 3 cut(s) 336, 557, 925
SsiI CCGC 7 cut(s) 723, 743, 764, 785, 805, 808, 849
SspMI CTAG 3 cut(s) 35, 135, 931
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 3 cut(s) 549, 602, 842
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 821
TaiI ACGT 2 cut(s) 101, 134
TaqI TCGA 5 cut(s) 531, 626, 631, 798, 879
TaqII GACCGA 1 cut(s) 226
TasI AATT 3 cut(s) 336, 557, 925
TauI GCSGC 3 cut(s) 788, 807, 810
TfiI GAWTC 5 cut(s) 254, 344, 370, 409, 731
Tru1I TTAA 1 cut(s) 662
Tru9I TTAA 1 cut(s) 662
TscAI CASTG 1 cut(s) 916
TseFI GTSAC 1 cut(s) 872
Tsp45I GTSAC 1 cut(s) 872
TspDTI ATGAA 3 cut(s) 606, 744, 897
TspGWI ACGGA 1 cut(s) 327
TspRI CASTG 1 cut(s) 916
XapI RAATTY 2 cut(s) 336, 557
XhoI CTCGAG 1 cut(s) 630
XspI CTAG 3 cut(s) 35, 135, 931
ZraI GACGTC 1 cut(s) 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.