FvH4_5g03210
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011031
Physical Location & Seq
Forward (+)
1907771 .. 1909327
1557 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g03210.t1

Sequence Viewer

Length: 930 bp
ATGGAGAACAGCTGGGATTTGCAAGCAGTAGTGAGAGGATGCAATAACAATCTTGAAGGGTTTGCTAATACCATGGAGTTCTCTCAATCATCTTGTGTTGGATTTGCTCCTGCAACCATTGAAGAAGATGATGATATTTTCCGGACCTTTCCTCAGCTGTTTGATACCACAACAGCTTTGGATGAATTGGAGGAGCTTTACAAGCCCTTCTACCCAAACTTGCAGCCTATTTCCCCTATCATTCCTAATCCTACCCCCGCCGCATCGGTTCTTCAGGAAGTCGCTAAAGAAGAAAAAGAAGAGCCAAAGAAGCTGAAAAGCTTGAAAGAATCCTCACCGATATGCAAAAGAAGCAGGAAGAATCAGAGCAAAAGGGTGGTGAAAGAGGTTAAGGAACAGGATCTGTTTTCGGACGTGTGGGCGTGGCGAAAATATGGTCAAAAACCCATCAAGGGATCGCCATATCCAAGAAGCTATTACAGGTGCAGTAGCTCAAAAGGATGTTCAGCAAGAAAACAAGTTGAGAGGAGCAATTCCAACCCAGAATTGTTCATCATAACCTTCACTGCAGAACACAACCATGATCATCCTACTCGGCGAAACTCTCTAGCTGGCAGCACTAGAACAAAGTTCCCAAAATCTCAGAACAATGAAAAGAGCAAACATGCATTTGTTCCTGTGAAAGAAGAAGCACATAGACTGTTGGAAGAGGATGAAGTTAGTCAAGAGAGCGCCACAGATATGATGTTGGGAGATGAAGCTGTTCCAAGCTTTGAAGATTTCGATAGAAAGTTGGAGGAATTGAACGAATTGATGGCGGAACCAGTCATGACAGATGATTTCTTTTCGGATCAGTACCTTTCAGATAGTTACTTTCCAAGTTCTTGGTTCCTTGGTCACTCTTCAAGTACTGTGACCGGAGCTTGCTGA

Protein Analysis

310

Amino Acids

35.22

Weight (kDa)

5.14

Isoelectric Point (pI)

61.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 138 - 195 8.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23550
fragaria_vesca FvH4_5g03210 FvH4_5g03210
malus_domestica MD06G1091200.v1.1 MD14G1112200.v1.1
prunus_persica Prupe.5G106700_v2.0.a1 Prupe.5G106700_v2.0.a1
pyrus_communis pycom06g08670 pycom14g10690
rosa_chinensis RchiOBHm_Chr7g0196571
rosa_laevigata RLG00000004005
rosa_multiflora Rmu_sc0006080.1_g000001
rosa_roxburghii Rroxscaffold_3G00259200
rosa_rugosa Rorug07G0034700 Rorug07G0034800
rosa_samantha Rh7AG158800 Rh7BG160800 Rh7CG167200 Rh7DG160000
rosa_wichuraiana Rw7G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 141
AciI CCGC 3 cut(s) 258, 261, 818
AclWI GGATC 3 cut(s) 408, 463, 858
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 2 cut(s) 857, 910
AfiI CCNNNNNNNGG 1 cut(s) 452
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 6 cut(s) 56, 122, 325, 776, 805, 906
AjiI CACGTC 1 cut(s) 415
AlwI GGATC 3 cut(s) 408, 463, 858
AlwNI CAGNNNCTG 1 cut(s) 403
Aor13HI TCCGGA 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 223, 615
ArsI GACNNNNNNTTYG 2 cut(s) 421, 453
Asp700I GAANNNNTTC 1 cut(s) 762
AspLEI GCGC 1 cut(s) 734
AspS9I GGNCC 1 cut(s) 144
AsuHPI GGTGA 2 cut(s) 327, 391
AvaII GGWCC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 153
BbvI GCAGC 2 cut(s) 235, 627
BccI CCATC 2 cut(s) 455, 808
BclI TGATCA 1 cut(s) 583
BfaI CTAG 2 cut(s) 608, 621
BfmI CTRYAG 1 cut(s) 567
BfoI RGCGCY 1 cut(s) 735
BisI GCNGC 3 cut(s) 224, 261, 616
BlsI GCNGC 3 cut(s) 225, 262, 617
BmcAI AGTACT 1 cut(s) 910
Bme18I GGWCC 1 cut(s) 144
BmgBI CACGTC 1 cut(s) 415
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 2 cut(s) 822, 890
BmsI GCATC 2 cut(s) 29, 272
Bpu10I CCTNAGC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 72, 892
BsaWI WCCGGW 2 cut(s) 141, 917
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse1I ACTGG 1 cut(s) 824
BseAI TCCGGA 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 72, 892
BseGI GGATG 5 cut(s) 44, 187, 506, 586, 718
BseLI CCNNNNNNNGG 1 cut(s) 452
BseMII CTCAG 2 cut(s) 167, 656
BseNI ACTGG 1 cut(s) 824
BseRI GAGGAG 2 cut(s) 206, 541
BseXI GCAGC 2 cut(s) 235, 627
BseYI CCCAGC 1 cut(s) 12
BsgI GTGCAG 1 cut(s) 505
BsiSI CCGG 2 cut(s) 142, 918
BslI CCNNNNNNNGG 1 cut(s) 452
Bsp13I TCCGGA 1 cut(s) 141
Bsp143I GATC 4 cut(s) 400, 455, 583, 850
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 3 cut(s) 258, 261, 818
BspCNI CTCAG 2 cut(s) 166, 655
BspEI TCCGGA 1 cut(s) 141
BspHI TCATGA 1 cut(s) 828
BspLI GGNNCC 2 cut(s) 822, 890
BspMAI CTGCAG 1 cut(s) 571
BspPI GGATC 3 cut(s) 408, 463, 858
BspQI GCTCTTC 1 cut(s) 294
BsrI ACTGG 1 cut(s) 824
BssECI CCNNGG 2 cut(s) 72, 892
BssMI GATC 4 cut(s) 400, 455, 583, 850
BssT1I CCWWGG 2 cut(s) 72, 892
Bst4CI ACNGT 2 cut(s) 702, 913
Bst6I CTCTTC 3 cut(s) 294, 702, 907
BstC8I GCNNGC 3 cut(s) 24, 613, 925
BstDEI CTNAG 2 cut(s) 153, 642
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 5 cut(s) 44, 187, 506, 586, 718
BstH2I RGCGCY 1 cut(s) 735
BstHHI GCGC 1 cut(s) 734
BstKTI GATC 4 cut(s) 403, 458, 586, 853
BstMBI GATC 4 cut(s) 400, 455, 583, 850
BstMWI GCNNNNNNNGC 3 cut(s) 202, 310, 351
BstNSI RCATGY 1 cut(s) 668
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 235, 627
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 885
BstYI RGATCY 1 cut(s) 400
BtgI CCRYGG 1 cut(s) 72
BtrI CACGTC 1 cut(s) 415
BtsCI GGATG 5 cut(s) 44, 187, 506, 586, 718
BtsI GCAGTG 1 cut(s) 564
BtsIMutI CAGTG 1 cut(s) 564
Cac8I GCNNGC 3 cut(s) 24, 613, 925
CaiI CAGNNNCTG 1 cut(s) 403
CciI TCATGA 1 cut(s) 828
CfoI GCGC 1 cut(s) 734
Cfr13I GGNCC 1 cut(s) 144
Csp6I GTAC 2 cut(s) 856, 909
CviAII CATG 4 cut(s) 73, 581, 665, 829
CviQI GTAC 2 cut(s) 856, 909
DdeI CTNAG 2 cut(s) 153, 642
DpnI GATC 4 cut(s) 402, 457, 585, 852
DpnII GATC 4 cut(s) 400, 455, 583, 850
Eam1104I CTCTTC 3 cut(s) 294, 702, 907
EarI CTCTTC 3 cut(s) 294, 702, 907
EciI GGCGGA 1 cut(s) 833
Eco130I CCWWGG 2 cut(s) 72, 892
Eco47I GGWCC 1 cut(s) 144
Eco57I CTGAAG 1 cut(s) 257
EcoT14I CCWWGG 2 cut(s) 72, 892
EcoT22I ATGCAT 1 cut(s) 670
ErhI CCWWGG 2 cut(s) 72, 892
FaeI CATG 4 cut(s) 76, 584, 668, 832
FatI CATG 4 cut(s) 72, 580, 664, 828
FauI CCCGC 1 cut(s) 265
FbaI TGATCA 1 cut(s) 583
Fnu4HI GCNGC 3 cut(s) 224, 261, 616
FokI GGATG 5 cut(s) 51, 194, 513, 573, 725
Fsp4HI GCNGC 3 cut(s) 224, 261, 616
FspBI CTAG 2 cut(s) 608, 621
GlaI GCGC 1 cut(s) 733
GluI GCNGC 3 cut(s) 224, 261, 616
GsaI CCCAGC 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 735
HapII CCGG 2 cut(s) 142, 918
HhaI GCGC 1 cut(s) 734
Hin1II CATG 4 cut(s) 76, 584, 668, 832
Hin6I GCGC 1 cut(s) 732
HinP1I GCGC 1 cut(s) 732
HindIII AAGCTT 2 cut(s) 319, 769
HinfI GANTC 2 cut(s) 329, 361
HpaII CCGG 2 cut(s) 142, 918
HphI GGTGA 2 cut(s) 327, 391
Hpy188I TCNGA 5 cut(s) 366, 412, 645, 850, 865
Hpy188III TCNNGA 5 cut(s) 53, 142, 275, 725, 829
HpyAV CCTTC 3 cut(s) 50, 217, 571
HpyCH4III ACNGT 2 cut(s) 702, 913
HpyCH4IV ACGT 1 cut(s) 414
HpyCH4V TGCA 8 cut(s) 22, 42, 113, 223, 345, 486, 569, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 202, 310, 351
HpyF3I CTNAG 2 cut(s) 153, 642
HpySE526I ACGT 1 cut(s) 414
Hsp92II CATG 4 cut(s) 76, 584, 668, 832
HspAI GCGC 1 cut(s) 732
Kpn2I TCCGGA 1 cut(s) 141
Ksp22I TGATCA 1 cut(s) 583
Kzo9I GATC 4 cut(s) 400, 455, 583, 850
LguI GCTCTTC 1 cut(s) 294
LmnI GCTCC 4 cut(s) 112, 193, 528, 920
Lsp1109I GCAGC 2 cut(s) 235, 627
LweI GCATC 2 cut(s) 29, 272
MaeI CTAG 2 cut(s) 608, 621
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 3 cut(s) 869, 896, 913
MalI GATC 4 cut(s) 402, 457, 585, 852
MboI GATC 4 cut(s) 400, 455, 583, 850
MflI RGATCY 1 cut(s) 400
MluCI AATT 5 cut(s) 185, 532, 545, 800, 809
MmeI TCCRAC 4 cut(s) 79, 561, 684, 774
MnlI CCTC 8 cut(s) 29, 162, 184, 343, 379, 519, 703, 790
Mph1103I ATGCAT 1 cut(s) 670
MroI TCCGGA 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 762
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 3 cut(s) 340, 579, 740
MspA1I CMGCKG 2 cut(s) 12, 157
MspI CCGG 2 cut(s) 142, 918
MwoI GCNNNNNNNGC 3 cut(s) 202, 310, 351
NcoI CCATGG 1 cut(s) 72
NdeII GATC 4 cut(s) 400, 455, 583, 850
NlaIII CATG 4 cut(s) 76, 584, 668, 832
NlaIV GGNNCC 2 cut(s) 822, 890
NmeAIII GCCGAG 1 cut(s) 574
NmuCI GTSAC 2 cut(s) 896, 913
NsiI ATGCAT 1 cut(s) 670
NspI RCATGY 1 cut(s) 668
PagI TCATGA 1 cut(s) 828
PciSI GCTCTTC 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 762
PfeI GAWTC 2 cut(s) 329, 361
PkrI GCNGC 3 cut(s) 225, 262, 617
PspFI CCCAGC 1 cut(s) 12
PspN4I GGNNCC 2 cut(s) 822, 890
PspPI GGNCC 1 cut(s) 144
PstI CTGCAG 1 cut(s) 571
PstNI CAGNNNCTG 1 cut(s) 403
PsuI RGATCY 1 cut(s) 400
PvuII CAGCTG 2 cut(s) 12, 157
RsaI GTAC 2 cut(s) 857, 910
RsaNI GTAC 2 cut(s) 856, 909
RseI CAYNNNNRTG 3 cut(s) 340, 579, 740
SapI GCTCTTC 1 cut(s) 294
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 3 cut(s) 224, 261, 616
Sau3AI GATC 4 cut(s) 400, 455, 583, 850
Sau96I GGNCC 1 cut(s) 144
ScaI AGTACT 1 cut(s) 910
SfaNI GCATC 2 cut(s) 29, 272
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 1 cut(s) 144
SmiMI CAYNNNNRTG 3 cut(s) 340, 579, 740
Sse9I AATT 5 cut(s) 185, 532, 545, 800, 809
SsiI CCGC 3 cut(s) 258, 261, 818
SspMI CTAG 2 cut(s) 608, 621
StyI CCWWGG 2 cut(s) 72, 892
TaaI ACNGT 2 cut(s) 702, 913
TaiI ACGT 1 cut(s) 417
TaqI TCGA 1 cut(s) 783
TasI AATT 5 cut(s) 185, 532, 545, 800, 809
TatI WGTACW 1 cut(s) 908
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 2 cut(s) 329, 361
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 2 cut(s) 896, 913
TseI GCWGC 2 cut(s) 223, 615
Tsp45I GTSAC 2 cut(s) 896, 913
TspDTI ATGAA 5 cut(s) 198, 541, 666, 729, 771
TspRI CASTG 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 144
XceI RCATGY 1 cut(s) 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XmnI GAANNNNTTC 1 cut(s) 762
XspI CTAG 2 cut(s) 608, 621
ZrmI AGTACT 1 cut(s) 910
Zsp2I ATGCAT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.