RLG00000004005
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011031
Physical Location & Seq
Forward (+)
55177261 .. 55178342
1082 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004005

Sequence Viewer

Length: 915 bp
ATGGAGAACTGGGATTTGCAAGCTGTGGTGAGAGGGTGCAAAAACACTCTCGAAGGCCTTGCTAATACCATGGAGTTCTCTCAATCATCTTGTCTTGGCTTTGCTCCTGTAACCATTGAAGAAGATGATGAGATTTTCCAGGCTTTTCCTCAACTGTTTGAAGCCACAACAGCTTTGGATGAATTGGAGGAGCTTTACAAGCCCTTCTACCCAATTTTGCAGCCTATTTCCCCACCAACCATTCCTACCGCCTCCGTATCGGTTCCGCAAGAAGTCACTGCAGAAGAAAAAGAGCCAAAGAAGCTGAAAAGCGTGAAAGAATCCTCACCCATGTCCAAAAGAAGGAAGAATCAGAGCAAGAGGGTGGTGAAAGAGGTTCAGGAGAAAGATCTGTTCTCGGATGTGTGGGCGTGGCGAAAATATGGGCAAAAACCCATCAAGGGATCGCCATATCCGAGGAGCTATTACCGGTGCAGTAGCTCGAAAGGTTGTTCAGCAAGAAAGCAAGTTGAAAGGAGCAACTCCAATCCAGAATTGTTCATCATAACCTACACTGCAGAACACAACCATGTTCATCCTACTCGGCGAAACTCTCTTGCTGGAAGCACAAGAACCAAGTTCCCCAAATCTCAGAAAAAAGAAGAGAGCAAAAATGAGTTTGTGCCTGTGAAAGAGGAAGCACAGAGACTGCCGGAAGAGGATGAAGTTAGTCAAGAGAGTGTGATGGATATGATGTTGGGAGATGAAGTTGTTCTGGGATTTGAGGATTTTGATCAAAAGTTGGAGGAATTGAGCGAATTGATGGCGGAACCAGTCATGGCCGATGGTTTCTTTTCGGATCAGTTTCCAGATAATTACTTTCCAAGTTCTTGGTTCCTTGGTCACTCTACAAGTACTGTAACTGGAGCTTGCTGA

Protein Analysis

305

Amino Acids

34.6

Weight (kDa)

5.04

Isoelectric Point (pI)

63.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 134 - 190 1.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23550
fragaria_vesca FvH4_5g03210 FvH4_5g03210
malus_domestica MD06G1091200.v1.1 MD14G1112200.v1.1
prunus_persica Prupe.5G106700_v2.0.a1 Prupe.5G106700_v2.0.a1
pyrus_communis pycom06g08670 pycom14g10690
rosa_chinensis RchiOBHm_Chr7g0196571
rosa_laevigata RLG00000004005
rosa_multiflora Rmu_sc0006080.1_g000001
rosa_roxburghii Rroxscaffold_3G00259200
rosa_rugosa Rorug07G0034700 Rorug07G0034800
rosa_samantha Rh7AG158800 Rh7BG160800 Rh7CG167200 Rh7DG160000
rosa_wichuraiana Rw7G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 249, 266, 806
AclWI GGATC 2 cut(s) 451, 846
AcoI YGGCCR 1 cut(s) 819
AfaI GTAC 1 cut(s) 895
AfiI CCNNNNNNNGG 2 cut(s) 342, 440
AgeI ACCGGT 1 cut(s) 468
AgsI TTSAA 3 cut(s) 119, 161, 512
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 7 cut(s) 23, 173, 193, 304, 462, 480, 908
AluI AGCT 7 cut(s) 23, 173, 193, 304, 462, 480, 908
Alw26I GTCTC 1 cut(s) 679
AlwI GGATC 2 cut(s) 451, 846
AlwNI CAGNNNCTG 1 cut(s) 688
AoxI GGCC 2 cut(s) 55, 819
ApeKI GCWGC 1 cut(s) 220
AsiGI ACCGGT 1 cut(s) 468
Asp700I GAANNNNTTC 1 cut(s) 750
AsuHPI GGTGA 3 cut(s) 40, 318, 379
BbvI GCAGC 1 cut(s) 232
BccI CCATC 4 cut(s) 443, 718, 796, 818
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 772
BcoDI GTCTC 1 cut(s) 679
BfmI CTRYAG 2 cut(s) 279, 555
BglII AGATCT 1 cut(s) 388
BisI GCNGC 1 cut(s) 221
BlsI GCNGC 1 cut(s) 222
BmcAI AGTACT 1 cut(s) 895
Bme1390I CCNGG 1 cut(s) 140
BmiI GGNNCC 3 cut(s) 264, 810, 875
BmrFI CCNGG 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 19
BsaBI GATNNNNATC 1 cut(s) 771
BsaJI CCNNGG 3 cut(s) 69, 455, 877
BsaWI WCCGGW 1 cut(s) 468
BsaXI ACNNNNNCTCC 2 cut(s) 732, 762
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 440
Bse118I RCCGGY 1 cut(s) 468
Bse1I ACTGG 3 cut(s) 14, 812, 907
Bse8I GATNNNNATC 1 cut(s) 771
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 3 cut(s) 69, 455, 877
BseGI GGATG 4 cut(s) 184, 406, 574, 706
BseJI GATNNNNATC 1 cut(s) 771
BseLI CCNNNNNNNGG 2 cut(s) 342, 440
BseMII CTCAG 1 cut(s) 644
BseNI ACTGG 3 cut(s) 14, 812, 907
BseRI GAGGAG 2 cut(s) 203, 472
BseXI GCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 2 cut(s) 57, 821
BshTI ACCGGT 1 cut(s) 468
BsiSI CCGG 2 cut(s) 469, 692
BslI CCNNNNNNNGG 2 cut(s) 342, 440
BsmAI GTCTC 1 cut(s) 679
BsnI GGCC 2 cut(s) 57, 821
Bsp143I GATC 4 cut(s) 388, 443, 772, 838
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 3 cut(s) 249, 266, 806
BspANI GGCC 2 cut(s) 57, 821
BspCNI CTCAG 1 cut(s) 643
BspLI GGNNCC 3 cut(s) 264, 810, 875
BspMAI CTGCAG 2 cut(s) 283, 559
BspPI GGATC 2 cut(s) 451, 846
BsrFI RCCGGY 1 cut(s) 468
BsrI ACTGG 3 cut(s) 14, 812, 907
BssAI RCCGGY 1 cut(s) 468
BssECI CCNNGG 3 cut(s) 69, 455, 877
BssMI GATC 4 cut(s) 388, 443, 772, 838
BssT1I CCWWGG 2 cut(s) 69, 877
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 156, 898
Bst6I CTCTTC 2 cut(s) 636, 690
BstC8I GCNNGC 2 cut(s) 21, 910
BstDEI CTNAG 1 cut(s) 630
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 4 cut(s) 184, 406, 574, 706
BstKTI GATC 4 cut(s) 391, 446, 775, 841
BstMAI GTCTC 1 cut(s) 679
BstMBI GATC 4 cut(s) 388, 443, 772, 838
BstMWI GCNNNNNNNGC 3 cut(s) 170, 199, 301
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 2 cut(s) 279, 555
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 388
BstXI CCANNNNNNTGG 1 cut(s) 870
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 2 cut(s) 57, 821
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 4 cut(s) 184, 406, 574, 706
BtsI GCAGTG 2 cut(s) 276, 552
BtsIMutI CAGTG 2 cut(s) 276, 552
Cac8I GCNNGC 2 cut(s) 21, 910
CaiI CAGNNNCTG 1 cut(s) 688
Cfr10I RCCGGY 1 cut(s) 468
Csp6I GTAC 1 cut(s) 894
CspAI ACCGGT 1 cut(s) 468
CviAII CATG 4 cut(s) 70, 331, 569, 817
CviQI GTAC 1 cut(s) 894
DdeI CTNAG 1 cut(s) 630
DpnI GATC 4 cut(s) 390, 445, 774, 840
DpnII GATC 4 cut(s) 388, 443, 772, 838
EaeI YGGCCR 1 cut(s) 819
Eam1104I CTCTTC 2 cut(s) 636, 690
EarI CTCTTC 2 cut(s) 636, 690
EciI GGCGGA 1 cut(s) 821
Eco130I CCWWGG 2 cut(s) 69, 877
Eco147I AGGCCT 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 69, 877
ErhI CCWWGG 2 cut(s) 69, 877
FaeI CATG 4 cut(s) 73, 334, 572, 820
FaiI YATR 8 cut(s) 71, 332, 423, 451, 545, 570, 731, 818
FatI CATG 4 cut(s) 69, 330, 568, 816
FbaI TGATCA 1 cut(s) 772
Fnu4HI GCNGC 1 cut(s) 221
FokI GGATG 4 cut(s) 191, 413, 561, 713
Fsp4HI GCNGC 1 cut(s) 221
GluI GCNGC 1 cut(s) 221
HaeIII GGCC 2 cut(s) 57, 821
HapII CCGG 2 cut(s) 469, 692
Hin1II CATG 4 cut(s) 73, 334, 572, 820
HinfI GANTC 2 cut(s) 320, 349
HpaII CCGG 2 cut(s) 469, 692
HphI GGTGA 3 cut(s) 40, 318, 379
Hpy188I TCNGA 5 cut(s) 354, 400, 456, 633, 838
Hpy188III TCNNGA 5 cut(s) 50, 380, 530, 713, 848
HpyAV CCTTC 3 cut(s) 47, 214, 336
HpyCH4III ACNGT 2 cut(s) 156, 898
HpyCH4V TGCA 6 cut(s) 19, 39, 220, 281, 474, 557
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 199, 301
HpyF3I CTNAG 1 cut(s) 630
Hsp92II CATG 4 cut(s) 73, 334, 572, 820
Ksp22I TGATCA 1 cut(s) 772
Kzo9I GATC 4 cut(s) 388, 443, 772, 838
LmnI GCTCC 5 cut(s) 109, 190, 459, 516, 905
Lsp1109I GCAGC 1 cut(s) 232
MaeIII GTNAC 4 cut(s) 109, 274, 881, 898
MalI GATC 4 cut(s) 390, 445, 774, 840
MboI GATC 4 cut(s) 388, 443, 772, 838
MboII GAAGA 6 cut(s) 131, 134, 296, 358, 653, 707
MflI RGATCY 1 cut(s) 388
MluCI AATT 6 cut(s) 182, 213, 533, 788, 797, 853
MmeI TCCRAC 1 cut(s) 762
MroXI GAANNNNTTC 1 cut(s) 750
MslI CAYNNNNRTG 1 cut(s) 567
MspI CCGG 2 cut(s) 469, 692
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 170, 199, 301
NcoI CCATGG 1 cut(s) 69
NdeII GATC 4 cut(s) 388, 443, 772, 838
NlaIII CATG 4 cut(s) 73, 334, 572, 820
NlaIV GGNNCC 3 cut(s) 264, 810, 875
NmeAIII GCCGAG 1 cut(s) 562
NmuCI GTSAC 2 cut(s) 274, 881
PceI AGGCCT 1 cut(s) 57
PcsI WCGNNNNNNNCGW 1 cut(s) 452
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 2 cut(s) 320, 349
PinAI ACCGGT 1 cut(s) 468
PkrI GCNGC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 264, 810, 875
PstI CTGCAG 2 cut(s) 283, 559
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 1 cut(s) 895
RsaNI GTAC 1 cut(s) 894
RseI CAYNNNNRTG 1 cut(s) 567
SatI GCNGC 1 cut(s) 221
Sau3AI GATC 4 cut(s) 388, 443, 772, 838
ScaI AGTACT 1 cut(s) 895
ScrFI CCNGG 1 cut(s) 140
SfcI CTRYAG 2 cut(s) 279, 555
SmiMI CAYNNNNRTG 1 cut(s) 567
Sse9I AATT 6 cut(s) 182, 213, 533, 788, 797, 853
SseBI AGGCCT 1 cut(s) 57
SsiI CCGC 3 cut(s) 249, 266, 806
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 2 cut(s) 69, 877
TaaI ACNGT 2 cut(s) 156, 898
TaqI TCGA 2 cut(s) 51, 482
TasI AATT 6 cut(s) 182, 213, 533, 788, 797, 853
TatI WGTACW 1 cut(s) 893
TfiI GAWTC 2 cut(s) 320, 349
TscAI CASTG 2 cut(s) 283, 559
TseFI GTSAC 2 cut(s) 274, 881
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 2 cut(s) 274, 881
TspDTI ATGAA 5 cut(s) 195, 529, 563, 717, 759
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 2 cut(s) 283, 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
XmnI GAANNNNTTC 1 cut(s) 750
ZrmI AGTACT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.