RchiOBHm_Chr7g0196571
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0011031
Physical Location & Seq
Reverse (-)
14580087 .. 14581208
1122 bp
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UTR
Exon/CDS
Intron
PRQ17584

Sequence Viewer

Length: 672 bp
ATGGAGAACTGGGATTTGCAAGCTGTGGTGAGAGGGTGCAAAAACAATCTTGAAGGCCTTACTCATACCATGGAGTTCTCTCAATCATCTTGTTTTGGCTTTGCTCCTGTAACCATTGAAGAAGATGATGAGATTTTCCAGGCTTTTCCTCAACTGTTTGAAGCCACAACAGCTTTGGATGAATTGGAGGAGCTTTACAAGCCCTTCTACCCAATTTTGCAGCCTATTTCCCCACCAACCATTCCTATCGCCTCCGTATCGGTTCCGCAAGAAGTCACTGCAGAAGAAAAAGAGCCAAAGAAGCTGAAAAGCGTGAAAGAATCCTCACCCATGTCCAAAAGAAGCAGGAAGAATCAGAGCAAGAGGGTGGTGCAAGAGGTTCAGGAGAAAGATCTGTTCTCGGATGTGTGGGCGTGGCGAAAATATGGGCAAAAACCCATCAAGGGATCGCCATATCCGAGGAGCTATTACCGGTGCAGTAGCTCGAAAGGATGTTCAGCAAGAAAGCAAGTTGAAAGGAGCAATTCCAATCCAGAATTGTTCATCATAACCTACACTGCAGAACACAACCATGTTCATCCTACTCGGCGAAACTCTCTTGCTGGAAGCACAAGAACCAAGTTCCCCAAATCTCAGAAAAAGAAAAGAGCAAAAATGAGTTTGTGCCTGTAA

Protein Analysis

223

Amino Acids

25.54

Weight (kDa)

9.29

Isoelectric Point (pI)

68.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 135 - 191 8.9e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23550
fragaria_vesca FvH4_5g03210 FvH4_5g03210
malus_domestica MD06G1091200.v1.1 MD14G1112200.v1.1
prunus_persica Prupe.5G106700_v2.0.a1 Prupe.5G106700_v2.0.a1
pyrus_communis pycom06g08670 pycom14g10690
rosa_chinensis RchiOBHm_Chr7g0196571
rosa_laevigata RLG00000004005
rosa_multiflora Rmu_sc0006080.1_g000001
rosa_roxburghii Rroxscaffold_3G00259200
rosa_rugosa Rorug07G0034700 Rorug07G0034800
rosa_samantha Rh7AG158800 Rh7BG160800 Rh7CG167200 Rh7DG160000
rosa_wichuraiana Rw7G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 266
AclWI GGATC 1 cut(s) 454
AfiI CCNNNNNNNGG 1 cut(s) 443
AgeI ACCGGT 1 cut(s) 471
AgsI TTSAA 4 cut(s) 53, 119, 161, 515
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 6 cut(s) 23, 173, 193, 304, 465, 483
AluI AGCT 6 cut(s) 23, 173, 193, 304, 465, 483
AlwI GGATC 1 cut(s) 454
AoxI GGCC 1 cut(s) 55
ApeKI GCWGC 1 cut(s) 220
AsiGI ACCGGT 1 cut(s) 471
AsuHPI GGTGA 2 cut(s) 40, 318
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 446
BciT130I CCWGG 1 cut(s) 140
BfmI CTRYAG 2 cut(s) 279, 558
BglII AGATCT 1 cut(s) 391
BisI GCNGC 1 cut(s) 221
BlsI GCNGC 1 cut(s) 222
Bme1390I CCNGG 1 cut(s) 140
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 140
BmrI ACTGGG 1 cut(s) 19
BmuI ACTGGG 1 cut(s) 19
BsaJI CCNNGG 2 cut(s) 69, 458
BsaWI WCCGGW 1 cut(s) 471
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse118I RCCGGY 1 cut(s) 471
Bse1I ACTGG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 2 cut(s) 69, 458
BseGI GGATG 4 cut(s) 184, 409, 497, 577
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 1 cut(s) 14
BseRI GAGGAG 2 cut(s) 203, 475
BseXI GCAGC 1 cut(s) 232
BsgI GTGCAG 1 cut(s) 496
BshFI GGCC 1 cut(s) 57
BshTI ACCGGT 1 cut(s) 471
BsiSI CCGG 1 cut(s) 472
BslI CCNNNNNNNGG 1 cut(s) 443
BsnI GGCC 1 cut(s) 57
Bsp143I GATC 2 cut(s) 391, 446
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 1 cut(s) 266
BspANI GGCC 1 cut(s) 57
BspCNI CTCAG 1 cut(s) 646
BspLI GGNNCC 1 cut(s) 264
BspMAI CTGCAG 2 cut(s) 283, 562
BspPI GGATC 1 cut(s) 454
BsrFI RCCGGY 1 cut(s) 471
BsrI ACTGG 1 cut(s) 14
BssAI RCCGGY 1 cut(s) 471
BssECI CCNNGG 2 cut(s) 69, 458
BssMI GATC 2 cut(s) 391, 446
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 156
BstC8I GCNNGC 1 cut(s) 21
BstDEI CTNAG 1 cut(s) 633
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 4 cut(s) 184, 409, 497, 577
BstKTI GATC 2 cut(s) 394, 449
BstMBI GATC 2 cut(s) 391, 446
BstMWI GCNNNNNNNGC 3 cut(s) 170, 199, 301
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BstSFI CTRYAG 2 cut(s) 279, 558
BstV1I GCAGC 1 cut(s) 232
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
BsuRI GGCC 1 cut(s) 57
BtgI CCRYGG 1 cut(s) 69
BtsCI GGATG 4 cut(s) 184, 409, 497, 577
BtsI GCAGTG 2 cut(s) 276, 555
BtsIMutI CAGTG 2 cut(s) 276, 555
Cac8I GCNNGC 1 cut(s) 21
Cfr10I RCCGGY 1 cut(s) 471
CspAI ACCGGT 1 cut(s) 471
CviAII CATG 3 cut(s) 70, 331, 572
DdeI CTNAG 1 cut(s) 633
DpnI GATC 2 cut(s) 393, 448
DpnII GATC 2 cut(s) 391, 446
Eco130I CCWWGG 1 cut(s) 69
Eco147I AGGCCT 1 cut(s) 57
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 3 cut(s) 73, 334, 575
FaiI YATR 7 cut(s) 66, 71, 332, 426, 454, 548, 573
FatI CATG 3 cut(s) 69, 330, 571
Fnu4HI GCNGC 1 cut(s) 221
FokI GGATG 4 cut(s) 191, 416, 504, 564
Fsp4HI GCNGC 1 cut(s) 221
GluI GCNGC 1 cut(s) 221
HaeIII GGCC 1 cut(s) 57
HapII CCGG 1 cut(s) 472
Hin1II CATG 3 cut(s) 73, 334, 575
HinfI GANTC 2 cut(s) 320, 352
HpaII CCGG 1 cut(s) 472
HphI GGTGA 2 cut(s) 40, 318
Hpy188I TCNGA 4 cut(s) 357, 403, 459, 636
Hpy188III TCNNGA 3 cut(s) 50, 383, 533
HpyAV CCTTC 2 cut(s) 47, 214
HpyCH4III ACNGT 1 cut(s) 156
HpyCH4V TGCA 7 cut(s) 19, 39, 220, 281, 373, 477, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 199, 301
HpyF3I CTNAG 1 cut(s) 633
Hsp92II CATG 3 cut(s) 73, 334, 575
Kzo9I GATC 2 cut(s) 391, 446
LmnI GCTCC 4 cut(s) 109, 190, 462, 519
LpnPI CCDG 8 cut(s) 120, 125, 152, 331, 368, 485, 546, 588
Lsp1109I GCAGC 1 cut(s) 232
MaeIII GTNAC 2 cut(s) 109, 274
MalI GATC 2 cut(s) 393, 448
MboI GATC 2 cut(s) 391, 446
MboII GAAGA 4 cut(s) 131, 134, 296, 361
MflI RGATCY 1 cut(s) 391
MluCI AATT 4 cut(s) 182, 213, 523, 536
MnlI CCTC 8 cut(s) 26, 159, 181, 262, 334, 357, 370, 453
MslI CAYNNNNRTG 1 cut(s) 570
MspI CCGG 1 cut(s) 472
MspR9I CCNGG 1 cut(s) 140
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 3 cut(s) 170, 199, 301
NcoI CCATGG 1 cut(s) 69
NdeII GATC 2 cut(s) 391, 446
NlaIII CATG 3 cut(s) 73, 334, 575
NlaIV GGNNCC 1 cut(s) 264
NmeAIII GCCGAG 1 cut(s) 565
NmuCI GTSAC 1 cut(s) 274
PceI AGGCCT 1 cut(s) 57
PcsI WCGNNNNNNNCGW 1 cut(s) 455
PfeI GAWTC 2 cut(s) 320, 352
PinAI ACCGGT 1 cut(s) 471
PkrI GCNGC 1 cut(s) 222
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 264
PstI CTGCAG 2 cut(s) 283, 562
PsuI RGATCY 1 cut(s) 391
RseI CAYNNNNRTG 1 cut(s) 570
SatI GCNGC 1 cut(s) 221
Sau3AI GATC 2 cut(s) 391, 446
ScrFI CCNGG 1 cut(s) 140
SetI ASST 8 cut(s) 25, 175, 195, 306, 381, 467, 485, 554
SfcI CTRYAG 2 cut(s) 279, 558
SmiMI CAYNNNNRTG 1 cut(s) 570
Sse9I AATT 4 cut(s) 182, 213, 523, 536
SseBI AGGCCT 1 cut(s) 57
SsiI CCGC 1 cut(s) 266
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 138
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 1 cut(s) 156
TaqI TCGA 1 cut(s) 485
TasI AATT 4 cut(s) 182, 213, 523, 536
TfiI GAWTC 2 cut(s) 320, 352
TscAI CASTG 2 cut(s) 283, 562
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 1 cut(s) 220
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 195, 532, 566
TspGWI ACGGA 1 cut(s) 244
TspRI CASTG 2 cut(s) 283, 562
XcmI CCANNNNNNNNNTGG 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.