MD14G1112200.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011031
Physical Location & Seq
Forward (+)
18037604 .. 18039324
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1112200.v1.1.491

Sequence Viewer

Length: 948 bp
ATGGAGAACTGGGATTTGCAAGCTGTGGTAAGAGGGTGCAATACCGATATTGATGAAGTCATGGAGGACTCCCAAATACCATATTTCTTTGCTCCTTTAAGCCTTCAGGAAGACGACGAGCTTTTTCAAGCTTATTTTCCTCAGGCCTTTGAAGCCACAACAACTTTAGCTGAATTGGAGGAGCTTTACAAGCCTTTTTACCCAGTTAATCAAATTACATCCCCACCAACTATTCTTGCCTCCTCCATATCTGTTCCCCAAGAAGTCATCACAGAAGCACCCAAGCTGATAAAAAACATGAAGGAATCAGCACCTATTTGTAAAAGAATAAGGAAGAATCAGAACAAAAGGGTTGTGAAAGAGGTTAAAGCAGAAGATCTCTTTTCGGATGTGTGGGCGTGGCGTAAGTATGGGCAAAAACCCATCAAGGGATCGCCACATCCGAGGAGCTATTACAGATGCAGCAGCTCAAAAGGATGTTCAGCAAGAAAACAAGTAGAAAGGAGCTGTTCCAATCCTGAAACGTTCATCATAACCTACACTGCAGAACACAACCATGTTCATCCAACTCGTCGAAACTCTCTTGCTGGCAGCACCAGAAGCAAGTTCCCTTCATCTAGAAACAAGGACAAATGTAGTAGTTCTTCGAATTCTCCAGCTACGCCTAATCTTGCCGCATCCATTGAAGATAACCGTGAGTTTGTGGAAAGCGCGGATATTAATATCGTAAAAGAGGAAGCGAAATTCTTGGAAGAGGATGAAATTAGTAATGAAAATGTCGTGTTGGGTACTATGGTGAGTGATGAAATGTTCCAAAGCTTAGAGGATTTGGATAGAAAGTTGGAACCAGTCATGGAAGGTTTGTTTTCGGATCAATTTTTGGACAATTTTTCAAGTCCTTGGTTCAATATTAATGGCCACTCTAGTACTGTAACCGGTGCTTGCTGA

Protein Analysis

316

Amino Acids

35.65

Weight (kDa)

5.05

Isoelectric Point (pI)

58.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 130 - 186 1.8e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011031)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G23550
fragaria_vesca FvH4_5g03210 FvH4_5g03210
malus_domestica MD06G1091200.v1.1 MD14G1112200.v1.1
prunus_persica Prupe.5G106700_v2.0.a1 Prupe.5G106700_v2.0.a1
pyrus_communis pycom06g08670 pycom14g10690
rosa_chinensis RchiOBHm_Chr7g0196571
rosa_laevigata RLG00000004005
rosa_multiflora Rmu_sc0006080.1_g000001
rosa_roxburghii Rroxscaffold_3G00259200
rosa_rugosa Rorug07G0034700 Rorug07G0034800
rosa_samantha Rh7AG158800 Rh7BG160800 Rh7CG167200 Rh7DG160000
rosa_wichuraiana Rw7G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 713
AciI CCGC 2 cut(s) 675, 713
AclI AACGTT 1 cut(s) 524
AclWI GGATC 2 cut(s) 439, 879
AcoI YGGCCR 1 cut(s) 916
AcsI RAATTY 2 cut(s) 649, 743
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 2 cut(s) 790, 928
AfiI CCNNNNNNNGG 1 cut(s) 428
AgeI ACCGGT 1 cut(s) 935
AgsI TTSAA 5 cut(s) 128, 152, 686, 894, 907
AlwI GGATC 2 cut(s) 439, 879
AoxI GGCC 2 cut(s) 144, 916
ApeKI GCWGC 3 cut(s) 462, 465, 591
ApoI RAATTY 2 cut(s) 649, 743
AseI ATTAAT 2 cut(s) 720, 912
AsiGI ACCGGT 1 cut(s) 935
AspLEI GCGC 1 cut(s) 713
AsuHPI GGTGA 1 cut(s) 808
AsuII TTCGAA 1 cut(s) 647
AxyI CCTNAGG 1 cut(s) 141
BaeI ACNNNNGTAYC 2 cut(s) 780, 813
BalI TGGCCA 1 cut(s) 918
BarI GAAGNNNNNNTAC 2 cut(s) 628, 660
BbsI GAAGAC 1 cut(s) 117
BbvI GCAGC 3 cut(s) 474, 477, 603
BccI CCATC 1 cut(s) 431
BfaI CTAG 2 cut(s) 618, 924
BfmI CTRYAG 1 cut(s) 543
BglII AGATCT 1 cut(s) 376
BisI GCNGC 4 cut(s) 463, 466, 592, 675
BlsI GCNGC 4 cut(s) 464, 467, 593, 676
BmcAI AGTACT 1 cut(s) 928
BmiI GGNNCC 1 cut(s) 846
BmrI ACTGGG 2 cut(s) 19, 197
BmsI GCATC 2 cut(s) 449, 686
BmuI ACTGGG 2 cut(s) 19, 197
BpiI GAAGAC 1 cut(s) 117
BpmI CTGGAG 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 647
BsaJI CCNNGG 2 cut(s) 443, 899
BsaWI WCCGGW 1 cut(s) 935
Bsc4I CCNNNNNNNGG 1 cut(s) 428
Bse118I RCCGGY 1 cut(s) 935
Bse1I ACTGG 3 cut(s) 14, 203, 848
Bse21I CCTNAGG 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 443, 899
BseGI GGATG 7 cut(s) 218, 394, 439, 482, 562, 677, 763
BseLI CCNNNNNNNGG 1 cut(s) 428
BseMII CTCAG 1 cut(s) 155
BseNI ACTGG 3 cut(s) 14, 203, 848
BseRI GAGGAG 3 cut(s) 194, 232, 460
BseXI GCAGC 3 cut(s) 474, 477, 603
Bsh1236I CGCG 1 cut(s) 713
BshFI GGCC 2 cut(s) 146, 918
BshTI ACCGGT 1 cut(s) 935
BsiSI CCGG 1 cut(s) 936
BslI CCNNNNNNNGG 1 cut(s) 428
BsnI GGCC 2 cut(s) 146, 918
Bsp119I TTCGAA 1 cut(s) 647
Bsp143I GATC 3 cut(s) 376, 431, 871
BspACI CCGC 2 cut(s) 675, 713
BspANI GGCC 2 cut(s) 146, 918
BspCNI CTCAG 1 cut(s) 154
BspFNI CGCG 1 cut(s) 713
BspLI GGNNCC 1 cut(s) 846
BspMAI CTGCAG 1 cut(s) 547
BspPI GGATC 2 cut(s) 439, 879
BspT104I TTCGAA 1 cut(s) 647
BsrFI RCCGGY 1 cut(s) 935
BsrI ACTGG 3 cut(s) 14, 203, 848
BssAI RCCGGY 1 cut(s) 935
BssECI CCNNGG 2 cut(s) 443, 899
BssMI GATC 3 cut(s) 376, 431, 871
BssT1I CCWWGG 1 cut(s) 899
Bst4CI ACNGT 2 cut(s) 695, 931
Bst6I CTCTTC 1 cut(s) 747
BstBI TTCGAA 1 cut(s) 647
BstC8I GCNNGC 3 cut(s) 21, 589, 943
BstDEI CTNAG 2 cut(s) 141, 820
BstF5I GGATG 7 cut(s) 218, 394, 439, 482, 562, 677, 763
BstFNI CGCG 1 cut(s) 713
BstHHI GCGC 1 cut(s) 713
BstKTI GATC 3 cut(s) 379, 434, 874
BstMBI GATC 3 cut(s) 376, 431, 871
BstMWI GCNNNNNNNGC 3 cut(s) 152, 190, 600
BstSFI CTRYAG 1 cut(s) 543
BstUI CGCG 1 cut(s) 713
BstV1I GCAGC 3 cut(s) 474, 477, 603
BstV2I GAAGAC 1 cut(s) 117
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
Bsu36I CCTNAGG 1 cut(s) 141
BsuRI GGCC 2 cut(s) 146, 918
BtsCI GGATG 7 cut(s) 218, 394, 439, 482, 562, 677, 763
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 1 cut(s) 540
Cac8I GCNNGC 3 cut(s) 21, 589, 943
CfoI GCGC 1 cut(s) 713
Cfr10I RCCGGY 1 cut(s) 935
Csp6I GTAC 2 cut(s) 789, 927
CspAI ACCGGT 1 cut(s) 935
CviAII CATG 4 cut(s) 61, 298, 557, 853
CviQI GTAC 2 cut(s) 789, 927
DdeI CTNAG 2 cut(s) 141, 820
DpnI GATC 3 cut(s) 378, 433, 873
DpnII GATC 3 cut(s) 376, 431, 871
EaeI YGGCCR 1 cut(s) 916
Eam1104I CTCTTC 1 cut(s) 747
EarI CTCTTC 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 899
Eco147I AGGCCT 1 cut(s) 146
Eco57I CTGAAG 1 cut(s) 89
Eco81I CCTNAGG 1 cut(s) 141
EcoRI GAATTC 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 899
ErhI CCWWGG 1 cut(s) 899
FaeI CATG 4 cut(s) 64, 301, 560, 856
FaiI YATR 9 cut(s) 62, 82, 248, 299, 411, 533, 558, 794, 854
FatI CATG 4 cut(s) 60, 297, 556, 852
Fnu4HI GCNGC 4 cut(s) 463, 466, 592, 675
FokI GGATG 7 cut(s) 205, 401, 426, 489, 549, 664, 770
Fsp4HI GCNGC 4 cut(s) 463, 466, 592, 675
FspBI CTAG 2 cut(s) 618, 924
GlaI GCGC 1 cut(s) 712
GluI GCNGC 4 cut(s) 463, 466, 592, 675
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 2 cut(s) 146, 918
HapII CCGG 1 cut(s) 936
HhaI GCGC 1 cut(s) 713
Hin1II CATG 4 cut(s) 64, 301, 560, 856
Hin6I GCGC 1 cut(s) 711
HinP1I GCGC 1 cut(s) 711
HindIII AAGCTT 2 cut(s) 129, 817
HinfI GANTC 3 cut(s) 68, 305, 337
HpaII CCGG 1 cut(s) 936
HphI GGTGA 1 cut(s) 808
Hpy188I TCNGA 4 cut(s) 342, 388, 444, 871
Hpy188III TCNNGA 3 cut(s) 107, 518, 618
Hpy99I CGWCG 2 cut(s) 119, 576
HpyAV CCTTC 4 cut(s) 113, 295, 621, 851
HpyCH4III ACNGT 2 cut(s) 695, 931
HpyCH4IV ACGT 1 cut(s) 524
HpyCH4V TGCA 4 cut(s) 19, 39, 462, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 190, 600
HpyF3I CTNAG 2 cut(s) 141, 820
HpySE526I ACGT 1 cut(s) 524
Hsp92II CATG 4 cut(s) 64, 301, 560, 856
HspAI GCGC 1 cut(s) 711
Kzo9I GATC 3 cut(s) 376, 431, 871
LmnI GCTCC 4 cut(s) 97, 181, 447, 504
LpnPI CCDG 8 cut(s) 92, 128, 216, 531, 573, 610, 669, 861
Lsp1109I GCAGC 3 cut(s) 474, 477, 603
LweI GCATC 2 cut(s) 449, 686
MaeI CTAG 2 cut(s) 618, 924
MaeII ACGT 1 cut(s) 524
MaeIII GTNAC 1 cut(s) 931
MalI GATC 3 cut(s) 378, 433, 873
MboI GATC 3 cut(s) 376, 431, 871
MboII GAAGA 6 cut(s) 122, 346, 386, 636, 698, 764
MflI RGATCY 1 cut(s) 376
MlsI TGGCCA 1 cut(s) 918
MluCI AATT 7 cut(s) 173, 213, 649, 743, 762, 875, 886
MluNI TGGCCA 1 cut(s) 918
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 2 cut(s) 590, 822
Mox20I TGGCCA 1 cut(s) 918
MscI TGGCCA 1 cut(s) 918
MseI TTAA 5 cut(s) 98, 207, 366, 720, 912
MslI CAYNNNNRTG 1 cut(s) 555
Msp20I TGGCCA 1 cut(s) 918
MspI CCGG 1 cut(s) 936
MvnI CGCG 1 cut(s) 713
MwoI GCNNNNNNNGC 3 cut(s) 152, 190, 600
NdeII GATC 3 cut(s) 376, 431, 871
NlaIII CATG 4 cut(s) 64, 301, 560, 856
NlaIV GGNNCC 1 cut(s) 846
NspV TTCGAA 1 cut(s) 647
PceI AGGCCT 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 440
PfeI GAWTC 2 cut(s) 305, 337
PinAI ACCGGT 1 cut(s) 935
PkrI GCNGC 4 cut(s) 464, 467, 593, 676
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
PshBI ATTAAT 2 cut(s) 720, 912
Psp1406I AACGTT 1 cut(s) 524
PspN4I GGNNCC 1 cut(s) 846
PstI CTGCAG 1 cut(s) 547
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 2 cut(s) 790, 928
RsaNI GTAC 2 cut(s) 789, 927
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 5 cut(s) 98, 207, 366, 720, 912
SatI GCNGC 4 cut(s) 463, 466, 592, 675
Sau3AI GATC 3 cut(s) 376, 431, 871
ScaI AGTACT 1 cut(s) 928
SchI GAGTC 1 cut(s) 62
SfaNI GCATC 2 cut(s) 449, 686
SfcI CTRYAG 1 cut(s) 543
SfuI TTCGAA 1 cut(s) 647
SmiMI CAYNNNNRTG 1 cut(s) 555
Sse9I AATT 7 cut(s) 173, 213, 649, 743, 762, 875, 886
SseBI AGGCCT 1 cut(s) 146
SsiI CCGC 2 cut(s) 675, 713
SspI AATATT 1 cut(s) 910
SspMI CTAG 2 cut(s) 618, 924
StuI AGGCCT 1 cut(s) 146
StyI CCWWGG 1 cut(s) 899
TaaI ACNGT 2 cut(s) 695, 931
TaiI ACGT 1 cut(s) 527
TaqI TCGA 2 cut(s) 574, 647
TasI AATT 7 cut(s) 173, 213, 649, 743, 762, 875, 886
TatI WGTACW 1 cut(s) 926
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 2 cut(s) 305, 337
Tru1I TTAA 5 cut(s) 98, 207, 366, 720, 912
Tru9I TTAA 5 cut(s) 98, 207, 366, 720, 912
TscAI CASTG 1 cut(s) 547
TseI GCWGC 3 cut(s) 462, 465, 591
TspDTI ATGAA 8 cut(s) 69, 314, 517, 551, 603, 774, 786, 819
TspRI CASTG 1 cut(s) 547
VspI ATTAAT 2 cut(s) 720, 912
XapI RAATTY 2 cut(s) 649, 743
XbaI TCTAGA 1 cut(s) 617
XspI CTAG 2 cut(s) 618, 924
ZrmI AGTACT 1 cut(s) 928
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.