AT4G24050

short-chain dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009222
Physical Location & Seq
Forward (+)
12497055 .. 12500326
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24050.2

Sequence Viewer

Length: 999 bp
ATGATCGAGACTGGTAAATACCTTCTCGGCGCCGCCGGTGCTAGCGGTTTCGGCTCTAAATCCACCGCCGAGGAAGTTACCGAGAACTGCGACCTTCGTTCCATCACAGCGGTTATCACCGGTGCGACGTCGGGGATCGGAGCGGAGACGGCGAGAGTTCTGGCGAAACGAGGAGCGAGGTTGATTTTTCCGGCGAGGAATGTGAAAGCAGCGGAGGAAGCAAAAGAGAGAATCGTTTCTGAGTTTCCGGAGACAGAGATCGTCGTTATGAAGCTTGATCTTAGTTCTATCGCCTCCGTACGAAACTTCGTCGCCGATTTCGAGTCTCTTGATCTTCCTCTTAACCTTCTCATAAACAACGCAGGCAAGTTAGCACATGAACATGCAATATCTGAAGACGGGATCGAGATGACATTTGCTACTAATTATCTAGGCCATTTTCTCTTGACCAATCTTTTGCTAAACAAGATGATTCAAACGGCAGAGGAAACAGGAGTTCAAGGACGTATCGTTAACGTTACGTCCGGTATTCACGGATGGTTCTCCGGCGACTTGATCGAATATCTCCGACTCATTTCACAACCCAAGTGTCAATTCGATGCGACACGTGCGTATGCTCTCTCGAAGCTTGCCAACGTTTTGCATACCAAGGAGCTCTCTTCTAGACTCCAGAAAATTGGAGCGAATGTTACGGTAAATTGCGTACACCCAGGAGTTGTTAGAACCCGGTTAACAAGAGACCGAGAAGGTCTATTGACAGATCTCGTCTTCTTCCTGGCTTCCAAGCTCGTTAAGACCGTCCCTCAAGCAGCAGCAACGACGTGTTACGTGGCAACAAATCCAAGACTGGTGAACGTATCGGGAAAGTATTTTACGGATTGTAATGAAACGACACCGTCTGGACTCGGAACCAACTCCAGCGAAGCTACCAAATTATGGGCTGCTTCTGAGATTCTGGTTACTCAACATTCCAAGACCAGTTTCGACCCATGTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00830 ko01100 map00830 map01100
Pfam Domains
Protein Families
adh_short

Protein Analysis

332

Amino Acids

35.84

Weight (kDa)

6.67

Isoelectric Point (pI)

27.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 36 - 176 4e-26 short chain dehydrogenase
KR PF08659 37 - 128 7.7e-10 KR domain
adh_short_C2 PF13561 41 - 175 2.8e-13 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 131
AccB1I GGYRCC 1 cut(s) 29
AccB7I CCANNNNNTGG 1 cut(s) 936
AccBSI CCGCTC 1 cut(s) 143
AccIII TCCGGA 1 cut(s) 247
AciI CCGC 6 cut(s) 33, 45, 66, 110, 143, 212
AclI AACGTT 2 cut(s) 516, 636
AclWI GGATC 2 cut(s) 143, 410
AcuI CTGAAG 1 cut(s) 414
AcvI CACGTG 1 cut(s) 608
AcyI GRCGYC 2 cut(s) 30, 128
AfaI GTAC 2 cut(s) 300, 705
AfiI CCNNNNNNNGG 1 cut(s) 936
AflIII ACRYGT 2 cut(s) 605, 821
AgeI ACCGGT 1 cut(s) 119
AgsI TTSAA 2 cut(s) 476, 500
AjiI CACGTC 1 cut(s) 822
AjnI CCWGG 2 cut(s) 709, 774
AjuI GAANNNNNNNTTGG 4 cut(s) 578, 610, 965, 997
AluBI AGCT 6 cut(s) 274, 628, 655, 787, 926, 996
AluI AGCT 6 cut(s) 274, 628, 655, 787, 926, 996
Alw21I GWGCWC 1 cut(s) 657
Alw26I GTCTC 5 cut(s) 2, 140, 245, 330, 732
AlwI GGATC 2 cut(s) 143, 410
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 1 cut(s) 433
ApeKI GCWGC 4 cut(s) 209, 809, 812, 941
ArsI GACNNNNNNTTYG 2 cut(s) 439, 471
AsiGI ACCGGT 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 235
AspLEI GCGC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 727
AsuHPI GGTGA 2 cut(s) 109, 862
AsuNHI GCTAGC 1 cut(s) 41
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 657
BbrPI CACGTG 1 cut(s) 608
BbsI GAAGAC 2 cut(s) 402, 760
Bbv12I GWGCWC 1 cut(s) 657
BbvI GCAGC 4 cut(s) 221, 821, 824, 928
BccI CCATC 2 cut(s) 110, 531
BceAI ACGGC 2 cut(s) 165, 495
BciT130I CCWGG 2 cut(s) 711, 776
BcnI CCSGG 1 cut(s) 727
BcoDI GTCTC 5 cut(s) 2, 140, 245, 330, 732
BfaI CTAG 3 cut(s) 42, 431, 663
BfoI RGCGCY 1 cut(s) 33
BglII AGATCT 1 cut(s) 760
BisI GCNGC 5 cut(s) 33, 210, 810, 813, 942
BlsI GCNGC 5 cut(s) 34, 211, 811, 814, 943
Bme1390I CCNGG 3 cut(s) 711, 727, 776
BmgBI CACGTC 1 cut(s) 822
BmiI GGNNCC 2 cut(s) 31, 910
BmrFI CCNGG 3 cut(s) 711, 727, 776
BmsI GCATC 1 cut(s) 589
BmtI GCTAGC 1 cut(s) 45
BpiI GAAGAC 2 cut(s) 402, 760
BpmI CTGGAG 2 cut(s) 653, 901
BpuEI CTTGAG 1 cut(s) 789
BpuMI CCSGG 1 cut(s) 727
BsaAI YACGTR 2 cut(s) 608, 829
BsaHI GRCGYC 2 cut(s) 30, 128
BsaI GGTCTC 1 cut(s) 732
BsaJI CCNNGG 3 cut(s) 69, 648, 709
BsaWI WCCGGW 3 cut(s) 119, 247, 524
BsaXI ACNNNNNCTCC 2 cut(s) 672, 702
Bsc4I CCNNNNNNNGG 1 cut(s) 936
Bse118I RCCGGY 2 cut(s) 35, 119
Bse1I ACTGG 3 cut(s) 16, 852, 978
BseAI TCCGGA 1 cut(s) 247
BseBI CCWGG 2 cut(s) 711, 776
BseDI CCNNGG 3 cut(s) 69, 648, 709
BseGI GGATG 1 cut(s) 542
BseLI CCNNNNNNNGG 1 cut(s) 936
BseMII CTCAG 2 cut(s) 231, 939
BseNI ACTGG 3 cut(s) 16, 852, 978
BseRI GAGGAG 1 cut(s) 186
BseXI GCAGC 4 cut(s) 221, 821, 824, 928
BshFI GGCC 1 cut(s) 435
BshNI GGYRCC 1 cut(s) 29
BshTI ACCGGT 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 657
BsiSI CCGG 7 cut(s) 36, 120, 191, 248, 525, 546, 727
BsiWI CGTACG 1 cut(s) 298
BslFI GGGAC 1 cut(s) 785
BslI CCNNNNNNNGG 1 cut(s) 936
BsmAI GTCTC 5 cut(s) 2, 140, 245, 330, 732
BsmBI CGTCTC 1 cut(s) 140
BsmFI GGGAC 1 cut(s) 785
BsnI GGCC 1 cut(s) 435
Bso31I GGTCTC 1 cut(s) 732
Bsp1286I GDGCHC 1 cut(s) 657
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 8 cut(s) 3, 135, 258, 277, 331, 402, 555, 760
BspACI CCGC 6 cut(s) 33, 45, 66, 110, 143, 212
BspANI GGCC 1 cut(s) 435
BspCNI CTCAG 2 cut(s) 232, 940
BspEI TCCGGA 1 cut(s) 247
BspLI GGNNCC 2 cut(s) 31, 910
BspOI GCTAGC 1 cut(s) 45
BspPI GGATC 2 cut(s) 143, 410
BspT107I GGYRCC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 732
BsrBI CCGCTC 1 cut(s) 143
BsrFI RCCGGY 2 cut(s) 35, 119
BsrI ACTGG 3 cut(s) 16, 852, 978
BssAI RCCGGY 2 cut(s) 35, 119
BssECI CCNNGG 3 cut(s) 69, 648, 709
BssMI GATC 8 cut(s) 3, 135, 258, 277, 331, 402, 555, 760
BssNI GRCGYC 2 cut(s) 30, 128
BssT1I CCWWGG 1 cut(s) 648
Bst2UI CCWGG 2 cut(s) 711, 776
Bst4CI ACNGT 3 cut(s) 694, 799, 897
Bst6I CTCTTC 1 cut(s) 664
BstACI GRCGYC 2 cut(s) 30, 128
BstBAI YACGTR 2 cut(s) 608, 829
BstC8I GCNNGC 3 cut(s) 43, 364, 630
BstDEI CTNAG 3 cut(s) 240, 281, 948
BstF5I GGATG 1 cut(s) 542
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 1 cut(s) 32
BstKTI GATC 8 cut(s) 6, 138, 261, 280, 334, 405, 558, 763
BstMAI GTCTC 5 cut(s) 2, 140, 245, 330, 732
BstMBI GATC 8 cut(s) 3, 135, 258, 277, 331, 402, 555, 760
BstMWI GCNNNNNNNGC 5 cut(s) 38, 51, 149, 218, 608
BstNI CCWGG 2 cut(s) 711, 776
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 3 cut(s) 709, 725, 774
BstV1I GCAGC 4 cut(s) 221, 821, 824, 928
BstV2I GAAGAC 2 cut(s) 402, 760
BstX2I RGATCY 1 cut(s) 760
BstXI CCANNNNNNTGG 1 cut(s) 677
BstYI RGATCY 1 cut(s) 760
BsuRI GGCC 1 cut(s) 435
BtrI CACGTC 1 cut(s) 822
BtsCI GGATG 1 cut(s) 542
Cac8I GCNNGC 3 cut(s) 43, 364, 630
CfoI GCGC 1 cut(s) 32
Cfr10I RCCGGY 2 cut(s) 35, 119
Csp6I GTAC 2 cut(s) 299, 704
CspAI ACCGGT 1 cut(s) 119
CviAII CATG 3 cut(s) 377, 383, 990
CviQI GTAC 2 cut(s) 299, 704
DdeI CTNAG 3 cut(s) 240, 281, 948
DinI GGCGCC 1 cut(s) 31
DpnI GATC 8 cut(s) 5, 137, 260, 279, 333, 404, 557, 762
DpnII GATC 8 cut(s) 3, 135, 258, 277, 331, 402, 555, 760
Eam1104I CTCTTC 1 cut(s) 664
EarI CTCTTC 1 cut(s) 664
Ecl136II GAGCTC 1 cut(s) 655
Eco130I CCWWGG 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 732
Eco53kI GAGCTC 1 cut(s) 655
Eco57I CTGAAG 1 cut(s) 414
Eco72I CACGTG 1 cut(s) 608
EcoICRI GAGCTC 1 cut(s) 655
EcoRII CCWGG 2 cut(s) 709, 774
EcoT14I CCWWGG 1 cut(s) 648
EcoT38I GRGCYC 1 cut(s) 657
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 648
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 3 cut(s) 380, 386, 993
FaiI YATR 8 cut(s) 269, 353, 378, 384, 615, 645, 937, 991
FaqI GGGAC 1 cut(s) 785
FatI CATG 3 cut(s) 376, 382, 989
Fnu4HI GCNGC 5 cut(s) 33, 210, 810, 813, 942
FokI GGATG 1 cut(s) 549
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 5 cut(s) 33, 210, 810, 813, 942
FspBI CTAG 3 cut(s) 42, 431, 663
GlaI GCGC 1 cut(s) 31
GluI GCNGC 5 cut(s) 33, 210, 810, 813, 942
GsuI CTGGAG 2 cut(s) 653, 901
HaeII RGCGCY 1 cut(s) 33
HaeIII GGCC 1 cut(s) 435
HapII CCGG 7 cut(s) 36, 120, 191, 248, 525, 546, 727
HhaI GCGC 1 cut(s) 32
Hin1I GRCGYC 2 cut(s) 30, 128
Hin1II CATG 3 cut(s) 380, 386, 993
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
HincII GTYRAC 2 cut(s) 514, 732
HindII GTYRAC 2 cut(s) 514, 732
HindIII AAGCTT 2 cut(s) 272, 626
HinfI GANTC 7 cut(s) 231, 323, 472, 570, 666, 903, 952
HpaI GTTAAC 2 cut(s) 514, 732
HpaII CCGG 7 cut(s) 36, 120, 191, 248, 525, 546, 727
HphI GGTGA 2 cut(s) 109, 862
Hpy166II GTNNAC 4 cut(s) 514, 706, 732, 853
Hpy188I TCNGA 6 cut(s) 140, 241, 394, 569, 908, 949
Hpy8I GTNNAC 4 cut(s) 514, 706, 732, 853
Hpy99I CGWCG 5 cut(s) 130, 133, 266, 314, 823
HpyAV CCTTC 4 cut(s) 32, 104, 356, 740
HpyCH4III ACNGT 3 cut(s) 694, 799, 897
HpyCH4IV ACGT 9 cut(s) 128, 505, 516, 521, 607, 636, 821, 828, 855
HpyCH4V TGCA 2 cut(s) 386, 643
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 51, 149, 218, 608
HpyF3I CTNAG 3 cut(s) 240, 281, 948
HpySE526I ACGT 9 cut(s) 128, 505, 516, 521, 607, 636, 821, 828, 855
Hsp92I GRCGYC 2 cut(s) 30, 128
Hsp92II CATG 3 cut(s) 380, 386, 993
HspAI GCGC 1 cut(s) 30
KasI GGCGCC 1 cut(s) 29
Kpn2I TCCGGA 1 cut(s) 247
KspAI GTTAAC 2 cut(s) 514, 732
Kzo9I GATC 8 cut(s) 3, 135, 258, 277, 331, 402, 555, 760
LmnI GCTCC 4 cut(s) 140, 173, 652, 680
Lsp1109I GCAGC 4 cut(s) 221, 821, 824, 928
LweI GCATC 1 cut(s) 589
MaeI CTAG 3 cut(s) 42, 431, 663
MaeII ACGT 9 cut(s) 128, 505, 516, 521, 607, 636, 821, 828, 855
MaeIII GTNAC 5 cut(s) 76, 517, 688, 824, 958
MalI GATC 8 cut(s) 5, 137, 260, 279, 333, 404, 557, 762
MbiI CCGCTC 1 cut(s) 143
MboI GATC 8 cut(s) 3, 135, 258, 277, 331, 402, 555, 760
MboII GAAGA 5 cut(s) 326, 407, 651, 760, 763
MflI RGATCY 1 cut(s) 760
MhlI GDGCHC 1 cut(s) 657
MluCI AATT 5 cut(s) 424, 593, 675, 697, 932
Mly113I GGCGCC 1 cut(s) 30
MlyI GAGTC 4 cut(s) 332, 564, 660, 897
MmeI TCCRAC 1 cut(s) 592
MnlI CCTC 9 cut(s) 64, 164, 171, 189, 208, 304, 348, 478, 813
MroI TCCGGA 1 cut(s) 247
MroXI GAANNNNTTC 1 cut(s) 235
MseI TTAA 4 cut(s) 342, 513, 731, 792
MslI CAYNNNNRTG 1 cut(s) 381
MspA1I CMGCKG 2 cut(s) 110, 212
MspI CCGG 7 cut(s) 36, 120, 191, 248, 525, 546, 727
MspR9I CCNGG 3 cut(s) 711, 727, 776
MvaI CCWGG 2 cut(s) 711, 776
MwoI GCNNNNNNNGC 5 cut(s) 38, 51, 149, 218, 608
NarI GGCGCC 1 cut(s) 30
NciI CCSGG 1 cut(s) 727
NdeII GATC 8 cut(s) 3, 135, 258, 277, 331, 402, 555, 760
NheI GCTAGC 1 cut(s) 41
NlaIII CATG 3 cut(s) 380, 386, 993
NlaIV GGNNCC 2 cut(s) 31, 910
NmeAIII GCCGAG 2 cut(s) 6, 94
NspI RCATGY 1 cut(s) 386
PcsI WCGNNNNNNNCGW 2 cut(s) 318, 795
PdmI GAANNNNTTC 1 cut(s) 235
PfeI GAWTC 3 cut(s) 231, 472, 952
Pfl23II CGTACG 1 cut(s) 298
PflFI GACNNNGTC 1 cut(s) 895
PflMI CCANNNNNTGG 1 cut(s) 936
PinAI ACCGGT 1 cut(s) 119
PkrI GCNGC 5 cut(s) 34, 211, 811, 814, 943
PleI GAGTC 4 cut(s) 331, 564, 660, 897
PluTI GGCGCC 1 cut(s) 33
PmaCI CACGTG 1 cut(s) 608
PmlI CACGTG 1 cut(s) 608
PpsI GAGTC 4 cut(s) 331, 564, 660, 897
Ppu21I YACGTR 2 cut(s) 608, 829
Psp124BI GAGCTC 1 cut(s) 657
Psp1406I AACGTT 2 cut(s) 516, 636
Psp6I CCWGG 2 cut(s) 709, 774
PspCI CACGTG 1 cut(s) 608
PspGI CCWGG 2 cut(s) 709, 774
PspLI CGTACG 1 cut(s) 298
PspN4I GGNNCC 2 cut(s) 31, 910
PsuI RGATCY 1 cut(s) 760
PsyI GACNNNGTC 1 cut(s) 895
RsaI GTAC 2 cut(s) 300, 705
RsaNI GTAC 2 cut(s) 299, 704
RseI CAYNNNNRTG 1 cut(s) 381
SacI GAGCTC 1 cut(s) 657
SaqAI TTAA 4 cut(s) 342, 513, 731, 792
SatI GCNGC 5 cut(s) 33, 210, 810, 813, 942
Sau3AI GATC 8 cut(s) 3, 135, 258, 277, 331, 402, 555, 760
SchI GAGTC 4 cut(s) 332, 564, 660, 897
ScrFI CCNGG 3 cut(s) 711, 727, 776
SduI GDGCHC 1 cut(s) 657
SfaNI GCATC 1 cut(s) 589
SfoI GGCGCC 1 cut(s) 31
SgrAI CRCCGGYG 2 cut(s) 35, 119
SmiMI CAYNNNNRTG 1 cut(s) 381
SmlI CTYRAG 1 cut(s) 804
SmoI CTYRAG 1 cut(s) 804
Sse9I AATT 5 cut(s) 424, 593, 675, 697, 932
SsiI CCGC 6 cut(s) 33, 45, 66, 110, 143, 212
SspDI GGCGCC 1 cut(s) 29
SspMI CTAG 3 cut(s) 42, 431, 663
SstI GAGCTC 1 cut(s) 657
StyD4I CCNGG 3 cut(s) 709, 725, 774
StyI CCWWGG 1 cut(s) 648
TaaI ACNGT 3 cut(s) 694, 799, 897
TaiI ACGT 9 cut(s) 131, 508, 519, 524, 610, 639, 824, 831, 858
TaqI TCGA 7 cut(s) 6, 321, 405, 558, 597, 623, 984
TaqII GACCGA 1 cut(s) 756
TasI AATT 5 cut(s) 424, 593, 675, 697, 932
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 3 cut(s) 231, 472, 952
Tru1I TTAA 4 cut(s) 342, 513, 731, 792
Tru9I TTAA 4 cut(s) 342, 513, 731, 792
TseI GCWGC 4 cut(s) 209, 809, 812, 941
TspDTI ATGAA 3 cut(s) 284, 393, 900
TspGWI ACGGA 3 cut(s) 286, 549, 890
Tth111I GACNNNGTC 1 cut(s) 895
Van91I CCANNNNNTGG 1 cut(s) 936
XbaI TCTAGA 1 cut(s) 662
XceI RCATGY 1 cut(s) 386
XmnI GAANNNNTTC 1 cut(s) 235
XspI CTAG 3 cut(s) 42, 431, 663
ZraI GACGTC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.