Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009222

Physical Location & Seq

Genomic Coordinates Forward (+)

25186122 .. 25189438

Gene Structure 3317 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1113700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1014 bp

ATGCTCGACACCGTCAAGTTCCTAATCGGCTCCCCCGGCGCCAGCGGCTACGGCTCAAAGAGTACAGCCGAACAAGTCACCGACGCCTCCCCCGATCTCCGCCACATCACCGCCATCATCACAGGTGCTACGTCAGGGATCGGAGCCGAGACGGCTCGAGTTTTAGCCAAGCGCGGGGCCAAGCTGGTCCTCCCGGCTCGAAGCCTCAAAGCTGCCGAGGATGCCAAGGCTCGAATCCTCTCCGAGTTTCCCGGCTCCGAGATAATCGTCATGGCTCTCGATCTCAGCTCCCTCGCTTCCGTCAGGAGCTTCGCTTCCGAGTTCGAGTCCCTCCGGCTGCCTCTCAATCTTCTCATAAATAATGCCGGAAAGTTCGCGCACGAGCACGCGATTTCCGAAGACGGAATCGAAATGACCTTTGCTACTAATTATCTCGGTCATTTTCTGTTGACGAAGCTGTTGCTCAACACAATGATTGAAACGGCTGCGGCTAGCGGCGTTCAAGGGCGAATCGTGAACGTGTCGTCGGCCATTCACGGCTGGTTTTCTGGCGATATGATCCGATATCTTGGCGAGATAAGCAGAAGTGGAAGCCAGTACGACGAGACACGTGCGTACGCGCTCTCGAAGCTTGCCAACGTCTTGCACACCAAGGAACTCGCTCGCAGACTCAAGGAAATGGAGGCAAACGTGACCGTTAACTGTGTGCATCCGGGTATTGTTAAGACCCGCCTCACCCGAGAACGTGAAGGTCTCGCCACAGATTTGGTGTTCCTGTTTGCTTCAAAGCTCTTGAAAACAATCCCTCAGGCTAGTGCGACGACATGTTATGTTGCGACTCATCCTAGGCTGGTGGGCGTGTCCGGGAAGTACTTTGCTGATTGCAACGAGACATTGCCGTCGAAGATGGGGTCGAACTTAAGCGAAGCTGCTGGATTATGGTCCGCCTCTGAAATCATGGTGTCCAAGGATTCCAGTGCTATCTTGGATCCAAGCCACCAGCTGGAGCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00830 ko01100 map00830 map01100

Pfam Domains

Protein Families

adh_short

Protein Analysis

338

Amino Acids

36.15

Weight (kDa)

7.16

Isoelectric Point (pI)

34.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
adh_short	PF00106	37 - 177	3.8e-26	short chain dehydrogenase
KR	PF08659	37 - 154	3.7e-10	KR domain
adh_short_C2	PF13561	42 - 251	1.5e-18	Enoyl-(Acyl carrier protein) reductase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009222)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64590 AT4G24050 AT4G24050
fragaria_vesca	FvH4_5g11450 FvH4_5g11450 FvH4_5g11450
malus_domestica	MD06G1113700.v1.1 MD14G1134700.v1.1
prunus_persica	Prupe.5G126400_v2.0.a1 Prupe.5G126400_v2.0.a1
pyrus_communis	pycom06g10620
rosa_chinensis	RchiOBHm_Chr7g0186391
rosa_laevigata	RLG00000004847
rosa_multiflora	Rmu_sc0000645.1_g000045
rosa_roxburghii	Rroxscaffold_3G00268170
rosa_rugosa	Rorug06G0473800 Rorug06G0473900
rosa_samantha	Rh7AG067500 Rh7CG080500 Rh7DG081100
rosa_wichuraiana	Rw7G006760 Rw7G006990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	898
AccB1I	GGYRCC	1 cut(s)	38
AccB7I	CCANNNNNTGG	1 cut(s)	1003
AccII	CGCG	4 cut(s)	174, 377, 389, 620
AciI	CCGC	8 cut(s)	45, 100, 111, 174, 488, 495, 730, 945
AclWI	GGATC	4 cut(s)	146, 553, 983, 996
AcoI	YGGCCR	1 cut(s)	528
AcvI	CACGTG	1 cut(s)	611
AcyI	GRCGYC	2 cut(s)	39, 84
AfaI	GTAC	4 cut(s)	64, 599, 617, 872
AfiI	CCNNNNNNNGG	2 cut(s)	174, 1003
AflII	CTTAAG	1 cut(s)	919
AflIII	ACRYGT	3 cut(s)	519, 608, 824
AgsI	TTSAA	4 cut(s)	479, 503, 786, 796
AluBI	AGCT	9 cut(s)	184, 212, 288, 309, 457, 631, 790, 929, 1003
AluI	AGCT	9 cut(s)	184, 212, 288, 309, 457, 631, 790, 929, 1003
Alw21I	GWGCWC	2 cut(s)	387, 1011
Alw26I	GTCTC	4 cut(s)	143, 599, 758, 884
AlwI	GGATC	4 cut(s)	146, 553, 983, 996
Ama87I	CYCGRG	2 cut(s)	156, 738
AoxI	GGCC	2 cut(s)	177, 528
ApeKI	GCWGC	4 cut(s)	212, 337, 485, 929
ArsI	GACNNNNNNTTYG	2 cut(s)	896, 928
AspA2I	CCTAGG	1 cut(s)	845
AspLEI	GCGC	4 cut(s)	41, 174, 379, 622
AspS9I	GGNCC	3 cut(s)	177, 187, 942
AsuC2I	CCSGG	5 cut(s)	36, 194, 252, 714, 865
AsuHPI	GGTGA	3 cut(s)	70, 100, 727
AsuNHI	GCTAGC	1 cut(s)	491
AvaI	CYCGRG	2 cut(s)	156, 738
AvaII	GGWCC	2 cut(s)	187, 942
AvrII	CCTAGG	1 cut(s)	845
AxyI	CCTNAGG	1 cut(s)	807
BamHI	GGATCC	1 cut(s)	988
BanI	GGYRCC	1 cut(s)	38
BauI	CACGAG	1 cut(s)	380
BbrPI	CACGTG	1 cut(s)	611
BbsI	GAAGAC	1 cut(s)	405
Bbv12I	GWGCWC	2 cut(s)	387, 1011
BbvI	GCAGC	4 cut(s)	199, 324, 472, 916
BccI	CCATC	2 cut(s)	122, 901
BceAI	ACGGC	5 cut(s)	67, 168, 498, 553, 883
BcgI	CGANNNNNNTGC	4 cut(s)	442, 476, 593, 627
BcnI	CCSGG	5 cut(s)	36, 194, 252, 714, 865
BcoDI	GTCTC	4 cut(s)	143, 599, 758, 884
BfaI	CTAG	3 cut(s)	492, 813, 846
BfoI	RGCGCY	1 cut(s)	42
BfrI	CTTAAG	1 cut(s)	919
BglI	GCCNNNNNGGC	1 cut(s)	152
BisI	GCNGC	7 cut(s)	46, 213, 338, 486, 489, 496, 930
BlnI	CCTAGG	1 cut(s)	845
BlsI	GCNGC	7 cut(s)	47, 214, 339, 487, 490, 497, 931
BmcAI	AGTACT	1 cut(s)	872
Bme1390I	CCNGG	5 cut(s)	36, 194, 252, 714, 865
Bme18I	GGWCC	2 cut(s)	187, 942
BmeT110I	CYCGRG	2 cut(s)	156, 738
BmgT120I	GGNCC	3 cut(s)	177, 187, 942
BmiI	GGNNCC	6 cut(s)	31, 40, 145, 178, 256, 990
BmrFI	CCNGG	5 cut(s)	36, 194, 252, 714, 865
BmsI	GCATC	2 cut(s)	211, 718
BmtI	GCTAGC	1 cut(s)	495
BpiI	GAAGAC	1 cut(s)	405
BpuEI	CTTGAG	1 cut(s)	656
BpuMI	CCSGG	5 cut(s)	36, 194, 252, 714, 865
BsaAI	YACGTR	1 cut(s)	611
BsaHI	GRCGYC	2 cut(s)	39, 84
BsaI	GGTCTC	1 cut(s)	758
BsaJI	CCNNGG	6 cut(s)	34, 216, 225, 651, 845, 966
Bsc4I	CCNNNNNNNGG	2 cut(s)	174, 1003
Bse1I	ACTGG	2 cut(s)	595, 975
Bse21I	CCTNAGG	1 cut(s)	807
Bse3DI	GCAATG	1 cut(s)	893
BseDI	CCNNGG	6 cut(s)	34, 216, 225, 651, 845, 966
BseGI	GGATG	3 cut(s)	226, 709, 841
BseLI	CCNNNNNNNGG	2 cut(s)	174, 1003
BseMI	GCAATG	1 cut(s)	893
BseMII	CTCAG	2 cut(s)	298, 821
BseNI	ACTGG	2 cut(s)	595, 975
BseXI	GCAGC	4 cut(s)	199, 324, 472, 916
Bsh1236I	CGCG	4 cut(s)	174, 377, 389, 620
BshFI	GGCC	2 cut(s)	179, 530
BshNI	GGYRCC	1 cut(s)	38
BsiHKAI	GWGCWC	2 cut(s)	387, 1011
BsiHKCI	CYCGRG	2 cut(s)	156, 738
BsiSI	CCGG	7 cut(s)	36, 194, 252, 334, 366, 713, 864
BsiWI	CGTACG	1 cut(s)	615
BslFI	GGGAC	1 cut(s)	313
BslI	CCNNNNNNNGG	2 cut(s)	174, 1003
BsmAI	GTCTC	4 cut(s)	143, 599, 758, 884
BsmBI	CGTCTC	1 cut(s)	143
BsmFI	GGGAC	1 cut(s)	313
BsnI	GGCC	2 cut(s)	179, 530
Bso31I	GGTCTC	1 cut(s)	758
BsoBI	CYCGRG	2 cut(s)	156, 738
Bsp1286I	GDGCHC	2 cut(s)	387, 1011
Bsp143I	GATC	5 cut(s)	94, 138, 280, 558, 988
BspACI	CCGC	8 cut(s)	45, 100, 111, 174, 488, 495, 730, 945
BspANI	GGCC	2 cut(s)	179, 530
BspCNI	CTCAG	2 cut(s)	297, 820
BspFNI	CGCG	4 cut(s)	174, 377, 389, 620
BspLI	GGNNCC	6 cut(s)	31, 40, 145, 178, 256, 990
BspOI	GCTAGC	1 cut(s)	495
BspPI	GGATC	4 cut(s)	146, 553, 983, 996
BspT107I	GGYRCC	1 cut(s)	38
BspTI	CTTAAG	1 cut(s)	919
BspTNI	GGTCTC	1 cut(s)	758
BsrDI	GCAATG	1 cut(s)	893
BsrI	ACTGG	2 cut(s)	595, 975
BssECI	CCNNGG	6 cut(s)	34, 216, 225, 651, 845, 966
BssMI	GATC	5 cut(s)	94, 138, 280, 558, 988
BssNI	GRCGYC	2 cut(s)	39, 84
BssSI	CACGAG	1 cut(s)	380
BssT1I	CCWWGG	4 cut(s)	225, 651, 845, 966
Bst2BI	CACGAG	1 cut(s)	380
Bst4CI	ACNGT	3 cut(s)	13, 697, 704
BstACI	GRCGYC	2 cut(s)	39, 84
BstAFI	CTTAAG	1 cut(s)	919
BstBAI	YACGTR	1 cut(s)	611
BstC8I	GCNNGC	5 cut(s)	43, 387, 493, 633, 664
BstDEI	CTNAG	2 cut(s)	284, 807
BstF5I	GGATG	3 cut(s)	226, 709, 841
BstFNI	CGCG	4 cut(s)	174, 377, 389, 620
BstH2I	RGCGCY	1 cut(s)	42
BstHHI	GCGC	4 cut(s)	41, 174, 379, 622
BstKTI	GATC	5 cut(s)	97, 141, 283, 561, 991
BstMAI	GTCTC	4 cut(s)	143, 599, 758, 884
BstMBI	GATC	5 cut(s)	94, 138, 280, 558, 988
BstMWI	GCNNNNNNNGC	7 cut(s)	36, 45, 51, 152, 221, 579, 628
BstNSI	RCATGY	1 cut(s)	828
BstSCI	CCNGG	5 cut(s)	34, 192, 250, 712, 863
BstUI	CGCG	4 cut(s)	174, 377, 389, 620
BstV1I	GCAGC	4 cut(s)	199, 324, 472, 916
BstV2I	GAAGAC	1 cut(s)	405
BstX2I	RGATCY	1 cut(s)	988
BstXI	CCANNNNNNTGG	1 cut(s)	766
BstYI	RGATCY	1 cut(s)	988
Bsu36I	CCTNAGG	1 cut(s)	807
BsuRI	GGCC	2 cut(s)	179, 530
BtsCI	GGATG	3 cut(s)	226, 709, 841
BtsIMutI	CAGTG	1 cut(s)	982
Cac8I	GCNNGC	5 cut(s)	43, 387, 493, 633, 664
CfoI	GCGC	4 cut(s)	41, 174, 379, 622
Cfr13I	GGNCC	3 cut(s)	177, 187, 942
CseI	GACGC	1 cut(s)	92
Csp6I	GTAC	4 cut(s)	63, 598, 616, 871
CviAII	CATG	3 cut(s)	271, 825, 958
CviQI	GTAC	4 cut(s)	63, 598, 616, 871
DdeI	CTNAG	2 cut(s)	284, 807
DinI	GGCGCC	1 cut(s)	40
DpnI	GATC	5 cut(s)	96, 140, 282, 560, 990
DpnII	GATC	5 cut(s)	94, 138, 280, 558, 988
DrdI	GACNNNNNNGTC	1 cut(s)	898
DseDI	GACNNNNNNGTC	1 cut(s)	898
EaeI	YGGCCR	1 cut(s)	528
EciI	GGCGGA	2 cut(s)	89, 934
Eco130I	CCWWGG	4 cut(s)	225, 651, 845, 966
Eco31I	GGTCTC	1 cut(s)	758
Eco32I	GATATC	1 cut(s)	566
Eco47I	GGWCC	2 cut(s)	187, 942
Eco72I	CACGTG	1 cut(s)	611
Eco81I	CCTNAGG	1 cut(s)	807
Eco88I	CYCGRG	2 cut(s)	156, 738
EcoRV	GATATC	1 cut(s)	566
EcoT14I	CCWWGG	4 cut(s)	225, 651, 845, 966
EgeI	GGCGCC	1 cut(s)	40
EheI	GGCGCC	1 cut(s)	40
ErhI	CCWWGG	4 cut(s)	225, 651, 845, 966
Esp3I	CGTCTC	1 cut(s)	143
FaeI	CATG	3 cut(s)	274, 828, 961
FaiI	YATR	7 cut(s)	272, 356, 557, 826, 831, 940, 959
FaqI	GGGAC	1 cut(s)	313
FatI	CATG	3 cut(s)	270, 824, 957
FauI	CCCGC	2 cut(s)	167, 737
Fnu4HI	GCNGC	7 cut(s)	46, 213, 338, 486, 489, 496, 930
FokI	GGATG	3 cut(s)	233, 696, 828
Fsp4HI	GCNGC	7 cut(s)	46, 213, 338, 486, 489, 496, 930
FspBI	CTAG	3 cut(s)	492, 813, 846
GlaI	GCGC	4 cut(s)	40, 173, 378, 621
GluI	GCNGC	7 cut(s)	46, 213, 338, 486, 489, 496, 930
HaeII	RGCGCY	1 cut(s)	42
HaeIII	GGCC	2 cut(s)	179, 530
HapII	CCGG	7 cut(s)	36, 194, 252, 334, 366, 713, 864
HgaI	GACGC	1 cut(s)	92
HhaI	GCGC	4 cut(s)	41, 174, 379, 622
Hin1I	GRCGYC	2 cut(s)	39, 84
Hin1II	CATG	3 cut(s)	274, 828, 961
Hin6I	GCGC	4 cut(s)	39, 172, 377, 620
HinP1I	GCGC	4 cut(s)	39, 172, 377, 620
HincII	GTYRAC	2 cut(s)	450, 700
HindII	GTYRAC	2 cut(s)	450, 700
HindIII	AAGCTT	1 cut(s)	629
HinfI	GANTC	7 cut(s)	234, 326, 405, 510, 669, 838, 971
HpaI	GTTAAC	1 cut(s)	700
HpaII	CCGG	7 cut(s)	36, 194, 252, 334, 366, 713, 864
HphI	GGTGA	3 cut(s)	70, 100, 727
Hpy166II	GTNNAC	3 cut(s)	450, 517, 700
Hpy188I	TCNGA	7 cut(s)	143, 244, 259, 319, 397, 563, 952
Hpy188III	TCNNGA	5 cut(s)	278, 304, 514, 625, 793
Hpy8I	GTNNAC	3 cut(s)	450, 517, 700
Hpy99I	CGWCG	5 cut(s)	86, 529, 605, 823, 904
HpyAV	CCTTC	1 cut(s)	743
HpyCH4III	ACNGT	3 cut(s)	13, 697, 704
HpyCH4IV	ACGT	6 cut(s)	131, 519, 610, 639, 690, 745
HpyCH4V	TGCA	3 cut(s)	646, 709, 885
HpyF10VI	GCNNNNNNNGC	7 cut(s)	36, 45, 51, 152, 221, 579, 628
HpyF3I	CTNAG	2 cut(s)	284, 807
HpySE526I	ACGT	6 cut(s)	131, 519, 610, 639, 690, 745
Hsp92I	GRCGYC	2 cut(s)	39, 84
Hsp92II	CATG	3 cut(s)	274, 828, 961
HspAI	GCGC	4 cut(s)	39, 172, 377, 620
KasI	GGCGCC	1 cut(s)	38
KspAI	GTTAAC	1 cut(s)	700
Kzo9I	GATC	5 cut(s)	94, 138, 280, 558, 988
LmnI	GCTCC	6 cut(s)	35, 143, 260, 293, 306, 1006
Lsp1109I	GCAGC	4 cut(s)	199, 324, 472, 916
LweI	GCATC	2 cut(s)	211, 718
MaeI	CTAG	3 cut(s)	492, 813, 846
MaeII	ACGT	6 cut(s)	131, 519, 610, 639, 690, 745
MaeIII	GTNAC	2 cut(s)	76, 691
MalI	GATC	5 cut(s)	96, 140, 282, 560, 990
MboI	GATC	5 cut(s)	94, 138, 280, 558, 988
MboII	GAAGA	3 cut(s)	341, 410, 916
MflI	RGATCY	1 cut(s)	988
MhlI	GDGCHC	2 cut(s)	387, 1011
MluCI	AATT	1 cut(s)	427
Mly113I	GGCGCC	1 cut(s)	39
MlyI	GAGTC	3 cut(s)	335, 663, 832
MseI	TTAA	3 cut(s)	699, 723, 920
MspA1I	CMGCKG	2 cut(s)	45, 1003
MspCI	CTTAAG	1 cut(s)	919
MspI	CCGG	7 cut(s)	36, 194, 252, 334, 366, 713, 864
MspR9I	CCNGG	5 cut(s)	36, 194, 252, 714, 865
MvnI	CGCG	4 cut(s)	174, 377, 389, 620
MwoI	GCNNNNNNNGC	7 cut(s)	36, 45, 51, 152, 221, 579, 628
NarI	GGCGCC	1 cut(s)	39
NciI	CCSGG	5 cut(s)	36, 194, 252, 714, 865
NdeII	GATC	5 cut(s)	94, 138, 280, 558, 988
NheI	GCTAGC	1 cut(s)	491
NlaIII	CATG	3 cut(s)	274, 828, 961
NlaIV	GGNNCC	6 cut(s)	31, 40, 145, 178, 256, 990
NmeAIII	GCCGAG	2 cut(s)	172, 241
NmuCI	GTSAC	2 cut(s)	76, 691
NspI	RCATGY	1 cut(s)	828
PaeR7I	CTCGAG	1 cut(s)	156
PciI	ACATGT	1 cut(s)	824
PcsI	WCGNNNNNNNCGW	2 cut(s)	90, 393
PfeI	GAWTC	4 cut(s)	234, 405, 510, 971
Pfl23II	CGTACG	1 cut(s)	615
PflFI	GACNNNGTC	1 cut(s)	11
PflMI	CCANNNNNTGG	1 cut(s)	1003
PfoI	TCCNGGA	1 cut(s)	863
PkrI	GCNGC	7 cut(s)	47, 214, 339, 487, 490, 497, 931
PleI	GAGTC	3 cut(s)	334, 663, 832
PluTI	GGCGCC	1 cut(s)	42
PmaCI	CACGTG	1 cut(s)	611
PmlI	CACGTG	1 cut(s)	611
PpsI	GAGTC	3 cut(s)	334, 663, 832
Ppu21I	YACGTR	1 cut(s)	611
PscI	ACATGT	1 cut(s)	824
PspCI	CACGTG	1 cut(s)	611
PspLI	CGTACG	1 cut(s)	615
PspN4I	GGNNCC	6 cut(s)	31, 40, 145, 178, 256, 990
PspPI	GGNCC	3 cut(s)	177, 187, 942
PspXI	VCTCGAGB	1 cut(s)	156
PsuI	RGATCY	1 cut(s)	988
PsyI	GACNNNGTC	1 cut(s)	11
PvuII	CAGCTG	1 cut(s)	1003
RsaI	GTAC	4 cut(s)	64, 599, 617, 872
RsaNI	GTAC	4 cut(s)	63, 598, 616, 871
SaqAI	TTAA	3 cut(s)	699, 723, 920
SatI	GCNGC	7 cut(s)	46, 213, 338, 486, 489, 496, 930
Sau3AI	GATC	5 cut(s)	94, 138, 280, 558, 988
Sau96I	GGNCC	3 cut(s)	177, 187, 942
ScaI	AGTACT	1 cut(s)	872
SchI	GAGTC	3 cut(s)	335, 663, 832
ScrFI	CCNGG	5 cut(s)	36, 194, 252, 714, 865
SduI	GDGCHC	2 cut(s)	387, 1011
SfaNI	GCATC	2 cut(s)	211, 718
SfoI	GGCGCC	1 cut(s)	40
Sfr274I	CTCGAG	1 cut(s)	156
SinI	GGWCC	2 cut(s)	187, 942
SlaI	CTCGAG	1 cut(s)	156
SmlI	CTYRAG	3 cut(s)	156, 671, 919
SmoI	CTYRAG	3 cut(s)	156, 671, 919
Sse9I	AATT	1 cut(s)	427
SsiI	CCGC	8 cut(s)	45, 100, 111, 174, 488, 495, 730, 945
SspDI	GGCGCC	1 cut(s)	38
SspMI	CTAG	3 cut(s)	492, 813, 846
StyD4I	CCNGG	5 cut(s)	34, 192, 250, 712, 863
StyI	CCWWGG	4 cut(s)	225, 651, 845, 966
TaaI	ACNGT	3 cut(s)	13, 697, 704
TaiI	ACGT	6 cut(s)	134, 522, 613, 642, 693, 748
TaqII	GACCGA	1 cut(s)	425
TasI	AATT	1 cut(s)	427
TatI	WGTACW	2 cut(s)	62, 870
TauI	GCSGC	3 cut(s)	48, 491, 498
TfiI	GAWTC	4 cut(s)	234, 405, 510, 971
Tru1I	TTAA	3 cut(s)	699, 723, 920
Tru9I	TTAA	3 cut(s)	699, 723, 920
TscAI	CASTG	1 cut(s)	982
TseFI	GTSAC	2 cut(s)	76, 691
TseI	GCWGC	4 cut(s)	212, 337, 485, 929
Tsp45I	GTSAC	2 cut(s)	76, 691
TspGWI	ACGGA	2 cut(s)	289, 417
TspRI	CASTG	1 cut(s)	982
Tth111I	GACNNNGTC	1 cut(s)	11
Van91I	CCANNNNNTGG	1 cut(s)	1003
Vha464I	CTTAAG	1 cut(s)	919
VpaK11BI	GGWCC	2 cut(s)	187, 942
XceI	RCATGY	1 cut(s)	828
XcmI	CCANNNNNNNNNTGG	1 cut(s)	982
XhoI	CTCGAG	1 cut(s)	156
XmaJI	CCTAGG	1 cut(s)	845
XspI	CTAG	3 cut(s)	492, 813, 846
ZrmI	AGTACT	1 cut(s)	872

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.