Rroxscaffold_3G00268170

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009222
Physical Location & Seq
Reverse (-)
61302646 .. 61304966
2321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00268170.1

Sequence Viewer

Length: 1011 bp
ATGCTCGAAACCGTGAAGTACCTGATCGGCTCCGTCGGCACCAGCGGTTACGGCTCCAAGAGCACGGCGGAGCAAGTCACCGACAGCTGCCCCGATCTCCGCTCCATCACCGCCATCATCACAGGTGCTACGTCGGGGATCGGAGCAGAGACGGCGCGAGTGTTAGCGAAGCGCGGCGCCAAGCTAGTCCTCCCCGCTCGGAGCCTTAAAGCCGCCGAGGACGCCAAGGCGCGAATCCTCTCCGAGTTTCCGGACTCGGAGATCACTGTCATGGCTCTTGATCTCAGCTCCCTTGCTTCCGTTAGAAACTTCGTGTCGGAGTTCGAGTCCCTCCACCTACCTCTTAATCTCCTCATAAACAACGCCGGTAAGTTCGCGCATGAGCATGCGATTTCCGAAGACGGAATAGAAATGACTTTTGCTACTAATTATCTGGGTCATTTTCTGTTGACGAAGCTGCTGCTGAACAGGATGATCCAAACAGCGAAGGCCACCGGCGTCCAAGGGCGAATCGTGAACGTGTCGTCTGCTATTCACGGCTGGTTTTCCGGCGATACAATCCGATACCTCGGAGAGATAAGCCGGAGCAGAAGCAGCCAATACGACGAGACACGTGCGTATGCGCTGTCGAAGCTCGCCAACGTTTTGCACACGAAGGAACTCGGTCGCAGGCTCAGGGAAATGGAGGCCAACGTGACTGTGAACTGTGTGCATCCGGGGATTGTTAGGACCCGACTCACCAGAGAACGTGAAGGCCTTGCTACTGATATGGTGTTCCTCTTTGCTTCCAAGCTCTTGAAAACAATACCTCAGGCTGCAGCCACAACATGTTACGTTGCGACTCATCCACGGCTGATCGGTGTGTCCGGGAAGTATTTTGCGGATTGCAACGAGACATCGCCGTCAAAATTGGGGTCTAATTTGGTGGAAGCTAGTCGGTTATGGTCTGCTTCTGAAGTCATGGTGTCCAAAGATTCCGGTGCTATTTTTGCAGATCAATTGAAGGACTAG

Protein Analysis

336

Amino Acids

36.42

Weight (kDa)

8.8

Isoelectric Point (pI)

30.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 37 - 177 8.4e-27 short chain dehydrogenase
KR PF08659 37 - 129 4.1e-10 KR domain
Epimerase PF01370 39 - 223 8.3e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 42 - 252 4.4e-18 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 901
AccB1I GGYRCC 2 cut(s) 38, 176
AccBSI CCGCTC 2 cut(s) 102, 197
AccII CGCG 4 cut(s) 157, 174, 232, 377
AccIII TCCGGA 1 cut(s) 250
AciI CCGC 8 cut(s) 45, 68, 100, 111, 174, 195, 213, 881
AclI AACGTT 1 cut(s) 642
AclWI GGATC 2 cut(s) 146, 469
AcuI CTGAAG 1 cut(s) 975
AcvI CACGTG 1 cut(s) 614
AcyI GRCGYC 3 cut(s) 177, 222, 498
AfaI GTAC 1 cut(s) 20
AflIII ACRYGT 3 cut(s) 519, 611, 827
AgsI TTSAA 2 cut(s) 799, 1003
AluBI AGCT 7 cut(s) 87, 184, 288, 457, 634, 793, 932
AluI AGCT 7 cut(s) 87, 184, 288, 457, 634, 793, 932
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 3 cut(s) 143, 602, 887
AlwI GGATC 2 cut(s) 146, 469
Aor13HI TCCGGA 1 cut(s) 250
AoxI GGCC 3 cut(s) 489, 687, 754
ApeKI GCWGC 6 cut(s) 87, 457, 460, 594, 815, 818
ArsI GACNNNNNNTTYG 2 cut(s) 899, 931
AspLEI GCGC 6 cut(s) 157, 174, 179, 232, 379, 625
AspS9I GGNCC 1 cut(s) 729
AsuC2I CCSGG 2 cut(s) 717, 868
AsuHPI GGTGA 3 cut(s) 70, 100, 730
AvaII GGWCC 1 cut(s) 729
AxyI CCTNAGG 1 cut(s) 810
BanI GGYRCC 2 cut(s) 38, 176
BbrPI CACGTG 1 cut(s) 614
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 6 cut(s) 74, 444, 447, 606, 802, 830
BccI CCATC 2 cut(s) 113, 122
BceAI ACGGC 6 cut(s) 67, 81, 168, 553, 866, 886
BcgI CGANNNNNNTGC 4 cut(s) 442, 476, 596, 630
BcnI CCSGG 2 cut(s) 717, 868
BcoDI GTCTC 3 cut(s) 143, 602, 887
BfaI CTAG 3 cut(s) 185, 933, 1009
BfmI CTRYAG 1 cut(s) 816
BfoI RGCGCY 1 cut(s) 180
BisI GCNGC 8 cut(s) 88, 175, 213, 458, 461, 595, 816, 819
BlsI GCNGC 8 cut(s) 89, 176, 214, 459, 462, 596, 817, 820
Bme1390I CCNGG 2 cut(s) 717, 868
Bme18I GGWCC 1 cut(s) 729
BmgT120I GGNCC 1 cut(s) 729
BmiI GGNNCC 6 cut(s) 31, 40, 55, 178, 203, 731
BmrFI CCNGG 2 cut(s) 717, 868
BmsI GCATC 1 cut(s) 721
BpiI GAAGAC 1 cut(s) 405
Bpu10I CCTNAGC 1 cut(s) 674
BpuMI CCSGG 2 cut(s) 717, 868
BsaAI YACGTR 1 cut(s) 614
BsaHI GRCGYC 3 cut(s) 177, 222, 498
BsaJI CCNNGG 6 cut(s) 216, 225, 502, 568, 716, 848
BsaWI WCCGGW 2 cut(s) 250, 977
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bse118I RCCGGY 2 cut(s) 365, 494
Bse21I CCTNAGG 1 cut(s) 810
BseAI TCCGGA 1 cut(s) 250
BseDI CCNNGG 6 cut(s) 216, 225, 502, 568, 716, 848
BseGI GGATG 3 cut(s) 477, 712, 844
BseMII CTCAG 3 cut(s) 298, 688, 824
BseRI GAGGAG 1 cut(s) 341
BseXI GCAGC 6 cut(s) 74, 444, 447, 606, 802, 830
Bsh1236I CGCG 4 cut(s) 157, 174, 232, 377
Bsh1285I CGRYCG 1 cut(s) 667
BshFI GGCC 3 cut(s) 491, 689, 756
BshNI GGYRCC 2 cut(s) 38, 176
BsiEI CGRYCG 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 8 cut(s) 251, 366, 495, 549, 583, 716, 867, 978
BslFI GGGAC 1 cut(s) 313
BsmAI GTCTC 3 cut(s) 143, 602, 887
BsmBI CGTCTC 1 cut(s) 143
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 3 cut(s) 491, 689, 756
Bsp1286I GDGCHC 1 cut(s) 65
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 8 cut(s) 24, 94, 138, 261, 280, 474, 855, 994
BspACI CCGC 8 cut(s) 45, 68, 100, 111, 174, 195, 213, 881
BspANI GGCC 3 cut(s) 491, 689, 756
BspCNI CTCAG 3 cut(s) 297, 687, 823
BspEI TCCGGA 1 cut(s) 250
BspFNI CGCG 4 cut(s) 157, 174, 232, 377
BspLI GGNNCC 6 cut(s) 31, 40, 55, 178, 203, 731
BspMAI CTGCAG 1 cut(s) 820
BspPI GGATC 2 cut(s) 146, 469
BspT107I GGYRCC 2 cut(s) 38, 176
BsrBI CCGCTC 2 cut(s) 102, 197
BsrFI RCCGGY 2 cut(s) 365, 494
BssAI RCCGGY 2 cut(s) 365, 494
BssECI CCNNGG 6 cut(s) 216, 225, 502, 568, 716, 848
BssMI GATC 8 cut(s) 24, 94, 138, 261, 280, 474, 855, 994
BssNI GRCGYC 3 cut(s) 177, 222, 498
BssT1I CCWWGG 2 cut(s) 225, 502
Bst4CI ACNGT 4 cut(s) 13, 268, 700, 707
BstACI GRCGYC 3 cut(s) 177, 222, 498
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 3 cut(s) 387, 636, 671
BstDEI CTNAG 3 cut(s) 284, 674, 810
BstDSI CCRYGG 1 cut(s) 848
BstF5I GGATG 3 cut(s) 477, 712, 844
BstFNI CGCG 4 cut(s) 157, 174, 232, 377
BstH2I RGCGCY 1 cut(s) 180
BstHHI GCGC 6 cut(s) 157, 174, 179, 232, 379, 625
BstKTI GATC 8 cut(s) 27, 97, 141, 264, 283, 477, 858, 997
BstMAI GTCTC 3 cut(s) 143, 602, 887
BstMBI GATC 8 cut(s) 24, 94, 138, 261, 280, 474, 855, 994
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 8 cut(s) 36, 51, 60, 152, 221, 594, 631, 989
BstNSI RCATGY 2 cut(s) 389, 831
BstSCI CCNGG 2 cut(s) 715, 866
BstSFI CTRYAG 1 cut(s) 816
BstUI CGCG 4 cut(s) 157, 174, 232, 377
BstV1I GCAGC 6 cut(s) 74, 444, 447, 606, 802, 830
BstV2I GAAGAC 1 cut(s) 405
Bsu36I CCTNAGG 1 cut(s) 810
BsuRI GGCC 3 cut(s) 491, 689, 756
BtgI CCRYGG 1 cut(s) 848
BtgZI GCGATG 1 cut(s) 882
BtsCI GGATG 3 cut(s) 477, 712, 844
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 3 cut(s) 387, 636, 671
CfoI GCGC 6 cut(s) 157, 174, 179, 232, 379, 625
Cfr10I RCCGGY 2 cut(s) 365, 494
Cfr13I GGNCC 1 cut(s) 729
CseI GACGC 2 cut(s) 230, 487
Csp6I GTAC 1 cut(s) 19
CviAII CATG 5 cut(s) 271, 380, 386, 828, 961
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 3 cut(s) 284, 674, 810
DinI GGCGCC 1 cut(s) 178
DpnI GATC 8 cut(s) 26, 96, 140, 263, 282, 476, 857, 996
DpnII GATC 8 cut(s) 24, 94, 138, 261, 280, 474, 855, 994
DrdI GACNNNNNNGTC 1 cut(s) 901
DseDI GACNNNNNNGTC 1 cut(s) 901
EciI GGCGGA 1 cut(s) 83
Eco130I CCWWGG 2 cut(s) 225, 502
Eco147I AGGCCT 1 cut(s) 756
Eco47I GGWCC 1 cut(s) 729
Eco57I CTGAAG 1 cut(s) 975
Eco72I CACGTG 1 cut(s) 614
Eco81I CCTNAGG 1 cut(s) 810
EcoO109I RGGNCCY 1 cut(s) 729
EcoT14I CCWWGG 2 cut(s) 225, 502
EgeI GGCGCC 1 cut(s) 178
EheI GGCGCC 1 cut(s) 178
ErhI CCWWGG 2 cut(s) 225, 502
Esp3I CGTCTC 1 cut(s) 143
FaeI CATG 5 cut(s) 274, 383, 389, 831, 964
FaiI YATR 9 cut(s) 272, 356, 381, 387, 621, 770, 829, 943, 962
FaqI GGGAC 1 cut(s) 313
FatI CATG 5 cut(s) 270, 379, 385, 827, 960
FauI CCCGC 1 cut(s) 202
Fnu4HI GCNGC 8 cut(s) 88, 175, 213, 458, 461, 595, 816, 819
FokI GGATG 3 cut(s) 484, 699, 831
Fsp4HI GCNGC 8 cut(s) 88, 175, 213, 458, 461, 595, 816, 819
FspBI CTAG 3 cut(s) 185, 933, 1009
GlaI GCGC 6 cut(s) 156, 173, 178, 231, 378, 624
GluI GCNGC 8 cut(s) 88, 175, 213, 458, 461, 595, 816, 819
HaeII RGCGCY 1 cut(s) 180
HaeIII GGCC 3 cut(s) 491, 689, 756
HapII CCGG 8 cut(s) 251, 366, 495, 549, 583, 716, 867, 978
HgaI GACGC 2 cut(s) 230, 487
HhaI GCGC 6 cut(s) 157, 174, 179, 232, 379, 625
Hin1I GRCGYC 3 cut(s) 177, 222, 498
Hin1II CATG 5 cut(s) 274, 383, 389, 831, 964
Hin6I GCGC 6 cut(s) 155, 172, 177, 230, 377, 623
HinP1I GCGC 6 cut(s) 155, 172, 177, 230, 377, 623
HincII GTYRAC 1 cut(s) 450
HindII GTYRAC 1 cut(s) 450
HinfI GANTC 7 cut(s) 234, 254, 326, 510, 735, 841, 974
HpaII CCGG 8 cut(s) 251, 366, 495, 549, 583, 716, 867, 978
HphI GGTGA 3 cut(s) 70, 100, 730
Hpy166II GTNNAC 3 cut(s) 450, 517, 703
Hpy188I TCNGA 9 cut(s) 143, 201, 244, 259, 319, 397, 563, 572, 955
Hpy188III TCNNGA 4 cut(s) 251, 278, 514, 796
Hpy8I GTNNAC 3 cut(s) 450, 517, 703
Hpy99I CGWCG 3 cut(s) 38, 136, 608
HpyAV CCTTC 4 cut(s) 481, 649, 746, 997
HpyCH4III ACNGT 4 cut(s) 13, 268, 700, 707
HpyCH4IV ACGT 7 cut(s) 131, 519, 613, 642, 693, 748, 834
HpyCH4V TGCA 5 cut(s) 649, 712, 818, 888, 992
HpyF10VI GCNNNNNNNGC 8 cut(s) 36, 51, 60, 152, 221, 594, 631, 989
HpyF3I CTNAG 3 cut(s) 284, 674, 810
HpySE526I ACGT 7 cut(s) 131, 519, 613, 642, 693, 748, 834
Hsp92I GRCGYC 3 cut(s) 177, 222, 498
Hsp92II CATG 5 cut(s) 274, 383, 389, 831, 964
HspAI GCGC 6 cut(s) 155, 172, 177, 230, 377, 623
KasI GGCGCC 1 cut(s) 176
Kpn2I TCCGGA 1 cut(s) 250
Kzo9I GATC 8 cut(s) 24, 94, 138, 261, 280, 474, 855, 994
LmnI GCTCC 8 cut(s) 35, 59, 70, 107, 143, 201, 293, 585
Lsp1109I GCAGC 6 cut(s) 74, 444, 447, 606, 802, 830
LweI GCATC 1 cut(s) 721
MaeI CTAG 3 cut(s) 185, 933, 1009
MaeII ACGT 7 cut(s) 131, 519, 613, 642, 693, 748, 834
MaeIII GTNAC 4 cut(s) 47, 76, 694, 830
MalI GATC 8 cut(s) 26, 96, 140, 263, 282, 476, 857, 996
MbiI CCGCTC 2 cut(s) 102, 197
MboI GATC 8 cut(s) 24, 94, 138, 261, 280, 474, 855, 994
MboII GAAGA 1 cut(s) 410
MfeI CAATTG 1 cut(s) 998
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 4 cut(s) 427, 908, 919, 998
Mly113I GGCGCC 1 cut(s) 177
MlyI GAGTC 4 cut(s) 248, 335, 729, 835
MmeI TCCRAC 1 cut(s) 297
MroI TCCGGA 1 cut(s) 250
MseI TTAA 2 cut(s) 207, 345
MslI CAYNNNNRTG 2 cut(s) 269, 384
MspA1I CMGCKG 2 cut(s) 45, 87
MspI CCGG 8 cut(s) 251, 366, 495, 549, 583, 716, 867, 978
MspR9I CCNGG 2 cut(s) 717, 868
MteI GCGCNGCGC 1 cut(s) 175
MunI CAATTG 1 cut(s) 998
MvnI CGCG 4 cut(s) 157, 174, 232, 377
MwoI GCNNNNNNNGC 8 cut(s) 36, 51, 60, 152, 221, 594, 631, 989
NarI GGCGCC 1 cut(s) 177
NciI CCSGG 2 cut(s) 717, 868
NdeII GATC 8 cut(s) 24, 94, 138, 261, 280, 474, 855, 994
NlaIII CATG 5 cut(s) 274, 383, 389, 831, 964
NlaIV GGNNCC 6 cut(s) 31, 40, 55, 178, 203, 731
NmeAIII GCCGAG 1 cut(s) 241
NmuCI GTSAC 2 cut(s) 76, 694
NspI RCATGY 2 cut(s) 389, 831
PaeI GCATGC 1 cut(s) 389
PceI AGGCCT 1 cut(s) 756
PciI ACATGT 1 cut(s) 827
PfeI GAWTC 3 cut(s) 234, 510, 974
PfoI TCCNGGA 1 cut(s) 866
PkrI GCNGC 8 cut(s) 89, 176, 214, 459, 462, 596, 817, 820
PleI GAGTC 4 cut(s) 248, 334, 729, 835
PluTI GGCGCC 1 cut(s) 180
PmaCI CACGTG 1 cut(s) 614
PmlI CACGTG 1 cut(s) 614
PpsI GAGTC 4 cut(s) 248, 334, 729, 835
Ppu21I YACGTR 1 cut(s) 614
PpuMI RGGWCCY 1 cut(s) 729
PscI ACATGT 1 cut(s) 827
Psp1406I AACGTT 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 729
PspCI CACGTG 1 cut(s) 614
PspN4I GGNNCC 6 cut(s) 31, 40, 55, 178, 203, 731
PspPI GGNCC 1 cut(s) 729
PspPPI RGGWCCY 1 cut(s) 729
PstI CTGCAG 1 cut(s) 820
PvuII CAGCTG 1 cut(s) 87
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
RseI CAYNNNNRTG 2 cut(s) 269, 384
SaqAI TTAA 2 cut(s) 207, 345
SatI GCNGC 8 cut(s) 88, 175, 213, 458, 461, 595, 816, 819
Sau3AI GATC 8 cut(s) 24, 94, 138, 261, 280, 474, 855, 994
Sau96I GGNCC 1 cut(s) 729
SchI GAGTC 4 cut(s) 248, 335, 729, 835
ScrFI CCNGG 2 cut(s) 717, 868
SduI GDGCHC 1 cut(s) 65
SfaNI GCATC 1 cut(s) 721
SfcI CTRYAG 1 cut(s) 816
SfoI GGCGCC 1 cut(s) 178
SgrAI CRCCGGYG 1 cut(s) 494
SinI GGWCC 1 cut(s) 729
SmiMI CAYNNNNRTG 2 cut(s) 269, 384
SphI GCATGC 1 cut(s) 389
Sse9I AATT 4 cut(s) 427, 908, 919, 998
SseBI AGGCCT 1 cut(s) 756
SsiI CCGC 8 cut(s) 45, 68, 100, 111, 174, 195, 213, 881
SspDI GGCGCC 1 cut(s) 176
SspMI CTAG 3 cut(s) 185, 933, 1009
StuI AGGCCT 1 cut(s) 756
StyD4I CCNGG 2 cut(s) 715, 866
StyI CCWWGG 2 cut(s) 225, 502
TaaI ACNGT 4 cut(s) 13, 268, 700, 707
TaiI ACGT 7 cut(s) 134, 522, 616, 645, 696, 751, 837
TaqI TCGA 3 cut(s) 6, 324, 629
TaqII GACCGA 1 cut(s) 653
TasI AATT 4 cut(s) 427, 908, 919, 998
TauI GCSGC 2 cut(s) 177, 215
TfiI GAWTC 3 cut(s) 234, 510, 974
Tru1I TTAA 2 cut(s) 207, 345
Tru9I TTAA 2 cut(s) 207, 345
TscAI CASTG 1 cut(s) 271
TseFI GTSAC 2 cut(s) 76, 694
TseI GCWGC 6 cut(s) 87, 457, 460, 594, 815, 818
Tsp45I GTSAC 2 cut(s) 76, 694
TspGWI ACGGA 3 cut(s) 22, 289, 417
TspRI CASTG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 729
XceI RCATGY 2 cut(s) 389, 831
XspI CTAG 3 cut(s) 185, 933, 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.