AT4G24580

Rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0007329
Physical Location & Seq
Reverse (-)
12687879 .. 12694656
6778 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24580.2

Sequence Viewer

Length: 2763 bp
ATGGCTAACAAAAACGCAGAATCATCTTCACAGCCTCCTCCTCATGTGCAGCCAAACCAACAACAGCAGCAGCAGCCACCGATTGCCAATGAACAAGAGCAGGAGCCTCATGGTGACACCTGCAGTATTCCTCCTGCGCAGTCTGGAAACACAGACTCGCGTTCCCGTGGTGGCAACACGGTTTTCAAGAGTGGACCGCTATCTATATCTTCAAAAGGGATTGGCTGGACATCATGGAAGAAAAGATGGTTTATTTTGACGCGTACTTCACTAGTTTTCTTTCGAAGTGACCCGAGTGCAGTCCAACAGAAGGGCAGTGAGGTCAATCTGACCCTTGGGGGAATTGATCTCAACAATTCAGGCAGTGTGGTTGTTAAGGCGGACAAGAAGCTGTTGACTGTTCTCTTTCCCGATGGTCGTGATGGACGGGCCTTTACACTTAAGGCTGACACTATGGAGGATCTACACGAGTGGAAAGCTGCTCTGGAAAACGCTCTGACACAAGCACCTAGTGCTTCCCATGTTATGGGTCAAAATGGTATTTTCAGGAATGATCATGCAGATCCTGCTGTTGGTGTTGATGAAAAGAAAGATGAGACACCAACAAAATCAACTGTCCTTGGAAGACCAGTATTACTTGCTCTAGAAGATGTTGATGGAGCTCCATCTTTCTTGGAAAAGGCCCTTAGATTTGTTGAAAATCATGGAGTCAGGATAGAAGGAATCTTGAGACAAGCTGCTGATGTTGATGATGTTGAACACCGAATTCGCGAATATGAGAAAGGAAAAAATGAGTTCAGTCCCGAGGAGGATGCTCATATTATTGCTGATTGCCTTAAGTATTTTCTCAGAGAGTTGCCTTCCTCTCCAGTGCCTGCATCTTGTTGCAACGCCCTTCTGGAAGCTTGCCGTACTGACCGTGGCAATAGGGTAAATGCTATGCGGGCAGCAATCTGTGAATCATTCCCTGAACCAAATCGTCGCCTATTGCAAAGAATCCTGATGATGATGCAAACTGTAGCCTCTAACAAAACTGTGAACCGGATGAACACAAACGCCGTTGCAGCTTGTATGGCACCTTTACTTCTTCGACCCCTTCTGGCTGGTGACTGTGAAATTGAAAATGATTTTGATGTCGGCGGTGATGGTTCTATGCAACTTCTGCAAGCAGCTGCTGCAGCGAACCATGCTCAGGCTATAGTGATAACATTATTAGAAGAATATGAAAGTATATTTGGAGAAGGTTCCTTGTCACCCGGCCTGTACTCTGACTCAGAAGAGAGTGGTAGTGGGACAGAGGAGGGATCTGATGATGAAGAGTATGATGATGACGATGATGGCTCACAGGGATCTGAGGACTACACGGATGAGGAAGAGGACCTTGAAAATGAATCTAATGGATCCTACAGTGAGAGTGCCGCCTCAGAAGATAAATATGCAGACAGCATTGATCCTGATGATCATAAGATAAATGATAATTTAAGCACTGAATCAAAATCCCCAAAAAGAAGTAAGGAGCCTAAGAAGCTTTTATCTGGCTCCAGACGATCTTCACTACCCCGACATGATGACGGCAAAAAGGATGAGGATATCGTGGTTAAGGGAGTTAATAATACAGAGGTAAAGGCTGTTGTAGAGGTCTCGACAAGTGAGGATAAGAATTCATCAACGTCAGATGTGGCATCTGACACTCAGAAACCGTCTAAGTTATCCGATGCACCGGGAGGAAGTAAAAGGCACTGGGGCCGTACCCCTGGGAAGAAAAACTTGTCAATGGAATCTATTGATTTCTCAGTGGAAGTGGACGAGGACAATGCTGACATTGAGAGACTTGAGTCAACCAAATTAGAGCTGCAAAGCAGAATTACAGAAGAGGTAAAAAGCAATGCAGTTCTACAGGCTAGTTTGGAGCGACGGAAGAAGGCTTTGTACGGGCGGCGCCAAGCACTAGAGCAAGATGTGGGAAGACTACAAGAACAGTTGCAGCAAGAGAGAGACAGGAAATTAGCTCTGGAAACAGGACTGAACATGTCAAAAGGGAATCAACCTATTCCAGAAACAATCGATGAAAATTTGAAGAAAGATCTGCAAGAGGTAGCTCAAGCGGAGGCTGATATTGCCAAGTTGGAACATAAGGTTGATGATCTTGAAAATAGGCTTGGTCACCATGACGGAAAAGCCTCTGGTTCTACGCATAGTGCCAGCAAAGAATCTCGGAAATTGCCAGAGCACAATGCGAAGATGAAGGAGAAACAAAAAGATACTGAAGCAGCCTCTACTCACATTTCTGAAAGATCAACGTCCAAGGATGGGCAAGGGGCTGCAAGAGAAAATGAGACAGAGAAGCAACAGGATTCACGGAGCAAAAGCTCGCAACAAGAAACATCACGTGGCAGCTCAAAACTGGTGGGCTTGTCAAAGAGGTCTGGCACAAAGGGAGAGGGAAGCACAACAACAACTTCTGCACTTTCCAAATTGACAATGAGACTCAACTTCCTCAAGGAGAGACGTAGTCAGATCGCGAACGAGCTCCAAAACATGGACAAAGGAAAAACCCTAGGGCAACCCAGTCCAACCTCGGGACAAAACCGTGTATCAGAGGAAACTGAAAAAGGATCAGGGTCGAACCAGGATCCAGACAGCAGCAAACTACAGAGCCCTCACATTCTTGACAGAGGAAGATCCGAGAACGGAGGAGACAGAGGCAGAGGATCATCTGGAGGAAACCATCCCAACACTACACCAAGGACCTTCTCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

920

Amino Acids

100.63

Weight (kDa)

5.22

Isoelectric Point (pI)

55.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 62 - 166 1.2e-11 PH domain
RhoGAP PF00620 222 - 365 2.6e-32 RhoGAP domain
Lzipper-MIP1 PF14389 645 - 720 1e-19 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 128
Acc16I TGCGCA 1 cut(s) 138
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 2 cut(s) 1075, 1938
AccB7I CCANNNNNTGG 2 cut(s) 526, 2539
AccII CGCG 4 cut(s) 160, 262, 771, 2522
AciI CCGC 7 cut(s) 197, 380, 943, 1140, 1419, 1936, 2105
AcsI RAATTY 3 cut(s) 765, 1660, 2071
AcuI CTGAAG 1 cut(s) 2286
AcvI CACGTG 1 cut(s) 2390
AcyI GRCGYC 1 cut(s) 1939
AdeI CACNNNGTG 1 cut(s) 512
AfaI GTAC 5 cut(s) 265, 913, 1265, 1750, 1931
AfiI CCNNNNNNNGG 7 cut(s) 310, 526, 572, 1192, 2310, 2539, 2579
AflII CTTAAG 2 cut(s) 440, 836
AflIII ACRYGT 2 cut(s) 260, 2028
AgsI TTSAA 8 cut(s) 187, 213, 698, 758, 1121, 1385, 2077, 2150
AhlI ACTAGT 1 cut(s) 271
AjnI CCWGG 2 cut(s) 1753, 2628
AjuI GAANNNNNNNTTGG 4 cut(s) 1218, 1250, 2142, 2174
Alw21I GWGCWC 3 cut(s) 664, 2232, 2532
Alw26I GTCTC 9 cut(s) 590, 724, 1645, 1822, 1989, 2330, 2479, 2500, 2691
AlwNI CAGNNNCTG 3 cut(s) 566, 875, 1175
Ama87I CYCGRG 3 cut(s) 292, 803, 2578
AoxI GGCC 4 cut(s) 429, 681, 1258, 1744
ApoI RAATTY 3 cut(s) 765, 1660, 2071
AspA2I CCTAGG 1 cut(s) 2557
AspLEI GCGC 2 cut(s) 139, 1941
AspS9I GGNCC 6 cut(s) 194, 429, 682, 1378, 1744, 2748
AsuC2I CCSGG 2 cut(s) 1257, 1722
AsuHPI GGTGA 5 cut(s) 125, 1118, 1154, 1245, 2156
AsuII TTCGAA 1 cut(s) 283
AvaI CYCGRG 3 cut(s) 292, 803, 2578
AvaII GGWCC 3 cut(s) 194, 1378, 2748
AvrII CCTAGG 1 cut(s) 2557
BamHI GGATCC 2 cut(s) 1400, 2632
BanI GGYRCC 2 cut(s) 1075, 1938
BanII GRGCYC 3 cut(s) 664, 2532, 2660
BarI GAAGNNNNNNTAC 2 cut(s) 1913, 1945
BauI CACGAG 1 cut(s) 467
BbrPI CACGTG 1 cut(s) 2390
BbsI GAAGAC 2 cut(s) 631, 1972
Bbv12I GWGCWC 3 cut(s) 664, 2232, 2532
BccI CCATC 9 cut(s) 240, 407, 416, 650, 673, 1139, 1331, 2303, 2736
BceAI ACGGC 4 cut(s) 894, 1043, 1588, 1731
BcgI CGANNNNNNTGC 4 cut(s) 794, 828, 1796, 1830
BciT130I CCWGG 2 cut(s) 1755, 2630
BclI TGATCA 2 cut(s) 553, 1459
BcnI CCSGG 2 cut(s) 1257, 1722
BcoDI GTCTC 9 cut(s) 590, 724, 1645, 1822, 1989, 2330, 2479, 2500, 2691
BcuI ACTAGT 1 cut(s) 271
BfaI CTAG 6 cut(s) 272, 510, 644, 1902, 1949, 2558
BfmI CTRYAG 7 cut(s) 121, 1017, 1176, 1197, 1405, 1895, 2651
BfoI RGCGCY 1 cut(s) 1942
BfrI CTTAAG 2 cut(s) 440, 836
BfuAI ACCTGC 1 cut(s) 128
BglII AGATCT 1 cut(s) 2083
BlnI CCTAGG 1 cut(s) 2557
Bme1390I CCNGG 4 cut(s) 1257, 1722, 1755, 2630
Bme18I GGWCC 3 cut(s) 194, 1378, 2748
BmeT110I CYCGRG 3 cut(s) 292, 803, 2578
BmgT120I GGNCC 6 cut(s) 194, 429, 682, 1378, 1744, 2748
BmiI GGNNCC 9 cut(s) 105, 1077, 1246, 1402, 1518, 1540, 1745, 1940, 2634
BmrFI CCNGG 4 cut(s) 1257, 1722, 1755, 2630
BmrI ACTGGG 2 cut(s) 1750, 2562
BmsI GCATC 5 cut(s) 802, 887, 999, 1691, 1705
BmuI ACTGGG 2 cut(s) 1750, 2562
BoxI GACNNNNGTC 1 cut(s) 1834
BpiI GAAGAC 2 cut(s) 631, 1972
BpmI CTGGAG 4 cut(s) 852, 1525, 2739, 2740
Bpu10I CCTNAGC 1 cut(s) 1191
Bpu14I TTCGAA 1 cut(s) 283
BpuEI CTTGAG 4 cut(s) 748, 1853, 2085, 2483
BpuMI CCSGG 2 cut(s) 1257, 1722
Bsa29I ATCGAT 1 cut(s) 2064
BsaAI YACGTR 1 cut(s) 2390
BsaHI GRCGYC 1 cut(s) 1939
BsaI GGTCTC 1 cut(s) 1645
BsaWI WCCGGW 1 cut(s) 1041
Bsc4I CCNNNNNNNGG 7 cut(s) 310, 526, 572, 1192, 2310, 2539, 2579
Bse1I ACTGG 5 cut(s) 629, 869, 1745, 2409, 2568
Bse3DI GCAATG 1 cut(s) 1891
BseBI CCWGG 2 cut(s) 1755, 2630
BseCI ATCGAT 1 cut(s) 2064
BseGI GGATG 6 cut(s) 817, 1050, 1372, 1588, 2314, 2728
BseLI CCNNNNNNNGG 7 cut(s) 310, 526, 572, 1192, 2310, 2539, 2579
BseMI GCAATG 1 cut(s) 1891
BseMII CTCAG 7 cut(s) 862, 1205, 1287, 1344, 1437, 1706, 1806
BseNI ACTGG 5 cut(s) 629, 869, 1745, 2409, 2568
BseRI GAGGAG 5 cut(s) 27, 30, 821, 1313, 2709
BsgI GTGCAG 3 cut(s) 68, 318, 2448
Bsh1236I CGCG 4 cut(s) 160, 262, 771, 2522
BshFI GGCC 4 cut(s) 431, 683, 1260, 1746
BshNI GGYRCC 2 cut(s) 1075, 1938
BshVI ATCGAT 1 cut(s) 2064
BsiHKAI GWGCWC 3 cut(s) 664, 2232, 2532
BsiHKCI CYCGRG 3 cut(s) 292, 803, 2578
BsiSI CCGG 3 cut(s) 1042, 1257, 1721
BslFI GGGAC 3 cut(s) 786, 1306, 2595
BslI CCNNNNNNNGG 7 cut(s) 310, 526, 572, 1192, 2310, 2539, 2579
BsmAI GTCTC 9 cut(s) 590, 724, 1645, 1822, 1989, 2330, 2479, 2500, 2691
BsmBI CGTCTC 1 cut(s) 2500
BsmFI GGGAC 3 cut(s) 786, 1306, 2595
BsnI GGCC 4 cut(s) 431, 683, 1260, 1746
Bso31I GGTCTC 1 cut(s) 1645
BsoBI CYCGRG 3 cut(s) 292, 803, 2578
Bsp119I TTCGAA 1 cut(s) 283
Bsp1286I GDGCHC 4 cut(s) 664, 2232, 2532, 2660
Bsp68I TCGCGA 2 cut(s) 771, 2522
BspACI CCGC 7 cut(s) 197, 380, 943, 1140, 1419, 1936, 2105
BspANI GGCC 4 cut(s) 431, 683, 1260, 1746
BspCNI CTCAG 7 cut(s) 861, 1204, 1286, 1345, 1436, 1705, 1805
BspDI ATCGAT 1 cut(s) 2064
BspFNI CGCG 4 cut(s) 160, 262, 771, 2522
BspLI GGNNCC 9 cut(s) 105, 1077, 1246, 1402, 1518, 1540, 1745, 1940, 2634
BspMAI CTGCAG 2 cut(s) 125, 1180
BspMI ACCTGC 1 cut(s) 128
BspT104I TTCGAA 1 cut(s) 283
BspT107I GGYRCC 2 cut(s) 1075, 1938
BspTI CTTAAG 2 cut(s) 440, 836
BspTNI GGTCTC 1 cut(s) 1645
BsrDI GCAATG 1 cut(s) 1891
BsrI ACTGG 5 cut(s) 629, 869, 1745, 2409, 2568
BssNI GRCGYC 1 cut(s) 1939
BssSI CACGAG 1 cut(s) 467
BssT1I CCWWGG 5 cut(s) 334, 619, 2304, 2557, 2744
Bst2BI CACGAG 1 cut(s) 467
Bst2UI CCWGG 2 cut(s) 1755, 2630
Bst6I CTCTTC 4 cut(s) 1272, 1311, 1368, 1866
BstACI GRCGYC 1 cut(s) 1939
BstAFI CTTAAG 2 cut(s) 440, 836
BstAPI GCANNNNNTGC 4 cut(s) 512, 566, 1162, 1175
BstBAI YACGTR 1 cut(s) 2390
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 6 cut(s) 876, 907, 945, 1167, 2203, 2372
BstDSI CCRYGG 2 cut(s) 166, 919
BstEII GGTNACC 1 cut(s) 2162
BstF5I GGATG 6 cut(s) 817, 1050, 1372, 1588, 2314, 2728
BstFNI CGCG 4 cut(s) 160, 262, 771, 2522
BstH2I RGCGCY 1 cut(s) 1942
BstHHI GCGC 2 cut(s) 139, 1941
BstMAI GTCTC 9 cut(s) 590, 724, 1645, 1822, 1989, 2330, 2479, 2500, 2691
BstNI CCWGG 2 cut(s) 1755, 2630
BstNSI RCATGY 1 cut(s) 2032
BstPAI GACNNNNGTC 1 cut(s) 1834
BstPI GGTNACC 1 cut(s) 2162
BstSCI CCNGG 4 cut(s) 1255, 1720, 1753, 2628
BstSFI CTRYAG 7 cut(s) 121, 1017, 1176, 1197, 1405, 1895, 2651
BstUI CGCG 4 cut(s) 160, 262, 771, 2522
BstV2I GAAGAC 2 cut(s) 631, 1972
BstX2I RGATCY 8 cut(s) 460, 562, 1304, 1349, 1400, 2083, 2632, 2681
BstYI RGATCY 8 cut(s) 460, 562, 1304, 1349, 1400, 2083, 2632, 2681
Bsu15I ATCGAT 1 cut(s) 2064
BsuRI GGCC 4 cut(s) 431, 683, 1260, 1746
BsuTUI ATCGAT 1 cut(s) 2064
BtgI CCRYGG 2 cut(s) 166, 919
BtsCI GGATG 6 cut(s) 817, 1050, 1372, 1588, 2314, 2728
BtsI GCAGTG 2 cut(s) 322, 370
BtsIMutI CAGTG 7 cut(s) 322, 370, 876, 1414, 1485, 1738, 1800
BtuMI TCGCGA 2 cut(s) 771, 2522
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 6 cut(s) 876, 907, 945, 1167, 2203, 2372
CaiI CAGNNNCTG 3 cut(s) 566, 875, 1175
CfoI GCGC 2 cut(s) 139, 1941
Cfr13I GGNCC 6 cut(s) 194, 429, 682, 1378, 1744, 2748
ClaI ATCGAT 1 cut(s) 2064
CseI GACGC 1 cut(s) 268
Csp6I GTAC 5 cut(s) 264, 912, 1264, 1749, 1930
CspCI CAANNNNNGTGG 2 cut(s) 2388, 2423
CviQI GTAC 5 cut(s) 264, 912, 1264, 1749, 1930
DinI GGCGCC 1 cut(s) 1940
DraIII CACNNNGTG 1 cut(s) 512
Eam1104I CTCTTC 4 cut(s) 1272, 1311, 1368, 1866
EarI CTCTTC 4 cut(s) 1272, 1311, 1368, 1866
EciI GGCGGA 1 cut(s) 395
Ecl136II GAGCTC 2 cut(s) 662, 2530
Eco130I CCWWGG 5 cut(s) 334, 619, 2304, 2557, 2744
Eco24I GRGCYC 3 cut(s) 664, 2532, 2660
Eco31I GGTCTC 1 cut(s) 1645
Eco32I GATATC 1 cut(s) 1591
Eco47I GGWCC 3 cut(s) 194, 1378, 2748
Eco53kI GAGCTC 2 cut(s) 662, 2530
Eco57I CTGAAG 1 cut(s) 2286
Eco72I CACGTG 1 cut(s) 2390
Eco88I CYCGRG 3 cut(s) 292, 803, 2578
Eco91I GGTNACC 1 cut(s) 2162
EcoICRI GAGCTC 2 cut(s) 662, 2530
EcoO109I RGGNCCY 3 cut(s) 682, 1378, 2748
EcoO65I GGTNACC 1 cut(s) 2162
EcoRI GAATTC 2 cut(s) 765, 1660
EcoRII CCWGG 2 cut(s) 1753, 2628
EcoRV GATATC 1 cut(s) 1591
EcoT14I CCWWGG 5 cut(s) 334, 619, 2304, 2557, 2744
EcoT38I GRGCYC 3 cut(s) 664, 2532, 2660
EgeI GGCGCC 1 cut(s) 1940
EheI GGCGCC 1 cut(s) 1940
ErhI CCWWGG 5 cut(s) 334, 619, 2304, 2557, 2744
Esp3I CGTCTC 1 cut(s) 2500
FalI AAGNNNNNCTT 4 cut(s) 1365, 1397, 1751, 1783
FaqI GGGAC 3 cut(s) 786, 1306, 2595
FauI CCCGC 1 cut(s) 936
FbaI TGATCA 2 cut(s) 553, 1459
FokI GGATG 6 cut(s) 824, 1057, 1379, 1595, 2321, 2715
FriOI GRGCYC 3 cut(s) 664, 2532, 2660
FspBI CTAG 6 cut(s) 272, 510, 644, 1902, 1949, 2558
FspI TGCGCA 1 cut(s) 138
GlaI GCGC 2 cut(s) 138, 1940
GsuI CTGGAG 4 cut(s) 852, 1525, 2739, 2740
HaeII RGCGCY 1 cut(s) 1942
HaeIII GGCC 4 cut(s) 431, 683, 1260, 1746
HapII CCGG 3 cut(s) 1042, 1257, 1721
HgaI GACGC 1 cut(s) 268
HhaI GCGC 2 cut(s) 139, 1941
Hin1I GRCGYC 1 cut(s) 1939
Hin6I GCGC 2 cut(s) 137, 1939
HinP1I GCGC 2 cut(s) 137, 1939
HincII GTYRAC 2 cut(s) 396, 1839
HindII GTYRAC 2 cut(s) 396, 1839
HindIII AAGCTT 2 cut(s) 903, 1526
HpaII CCGG 3 cut(s) 1042, 1257, 1721
HphI GGTGA 5 cut(s) 125, 1118, 1154, 1245, 2156
Hpy166II GTNNAC 5 cut(s) 194, 396, 1039, 1804, 1839
Hpy8I GTNNAC 5 cut(s) 194, 396, 1039, 1804, 1839
Hpy99I CGWCG 2 cut(s) 984, 1917
HpyAV CCTTC 9 cut(s) 304, 713, 870, 905, 1106, 1235, 1915, 2239, 2761
HpyCH4IV ACGT 4 cut(s) 1670, 2300, 2389, 2509
HpySE526I ACGT 4 cut(s) 1670, 2300, 2389, 2509
Hsp92I GRCGYC 1 cut(s) 1939
HspAI GCGC 2 cut(s) 137, 1939
KasI GGCGCC 1 cut(s) 1938
Ksp22I TGATCA 2 cut(s) 553, 1459
LmnI GCTCC 8 cut(s) 103, 659, 667, 1516, 1544, 1909, 2361, 2535
LweI GCATC 5 cut(s) 802, 887, 999, 1691, 1705
MaeI CTAG 6 cut(s) 272, 510, 644, 1902, 1949, 2558
MaeII ACGT 4 cut(s) 1670, 2300, 2389, 2509
MaeIII GTNAC 5 cut(s) 113, 287, 1106, 1251, 2162
MflI RGATCY 8 cut(s) 460, 562, 1304, 1349, 1400, 2083, 2632, 2681
MhlI GDGCHC 4 cut(s) 664, 2232, 2532, 2660
MluI ACGCGT 1 cut(s) 260
Mly113I GGCGCC 1 cut(s) 1939
MlyI GAGTC 5 cut(s) 149, 717, 1265, 1844, 2481
MmeI TCCRAC 3 cut(s) 328, 2106, 2597
MseI TTAA 6 cut(s) 375, 441, 837, 1481, 1599, 1608
MspA1I CMGCKG 1 cut(s) 1172
MspCI CTTAAG 2 cut(s) 440, 836
MspI CCGG 3 cut(s) 1042, 1257, 1721
MspR9I CCNGG 4 cut(s) 1257, 1722, 1755, 2630
MvaI CCWGG 2 cut(s) 1755, 2630
MvnI CGCG 4 cut(s) 160, 262, 771, 2522
NarI GGCGCC 1 cut(s) 1939
NciI CCSGG 2 cut(s) 1257, 1722
NlaIV GGNNCC 9 cut(s) 105, 1077, 1246, 1402, 1518, 1540, 1745, 1940, 2634
NmuCI GTSAC 5 cut(s) 113, 287, 1106, 1251, 2162
NruI TCGCGA 2 cut(s) 771, 2522
NsbI TGCGCA 1 cut(s) 138
NspI RCATGY 1 cut(s) 2032
NspV TTCGAA 1 cut(s) 283
PaqCI CACCTGC 1 cut(s) 128
PasI CCCWGGG 1 cut(s) 1754
PciI ACATGT 1 cut(s) 2028
PflFI GACNNNGTC 1 cut(s) 2511
PflMI CCANNNNNTGG 2 cut(s) 526, 2539
PleI GAGTC 5 cut(s) 149, 716, 1265, 1843, 2481
PluTI GGCGCC 1 cut(s) 1942
PmaCI CACGTG 1 cut(s) 2390
PmlI CACGTG 1 cut(s) 2390
PpsI GAGTC 5 cut(s) 149, 716, 1265, 1843, 2481
Ppu21I YACGTR 1 cut(s) 2390
PpuMI RGGWCCY 2 cut(s) 1378, 2748
PscI ACATGT 1 cut(s) 2028
PshAI GACNNNNGTC 1 cut(s) 1834
Psp124BI GAGCTC 2 cut(s) 664, 2532
Psp5II RGGWCCY 2 cut(s) 1378, 2748
Psp6I CCWGG 2 cut(s) 1753, 2628
PspCI CACGTG 1 cut(s) 2390
PspEI GGTNACC 1 cut(s) 2162
PspGI CCWGG 2 cut(s) 1753, 2628
PspN4I GGNNCC 9 cut(s) 105, 1077, 1246, 1402, 1518, 1540, 1745, 1940, 2634
PspPI GGNCC 6 cut(s) 194, 429, 682, 1378, 1744, 2748
PspPPI RGGWCCY 2 cut(s) 1378, 2748
PstI CTGCAG 2 cut(s) 125, 1180
PstNI CAGNNNCTG 3 cut(s) 566, 875, 1175
PsuI RGATCY 8 cut(s) 460, 562, 1304, 1349, 1400, 2083, 2632, 2681
PsyI GACNNNGTC 1 cut(s) 2511
PvuII CAGCTG 1 cut(s) 1172
RruI TCGCGA 2 cut(s) 771, 2522
RsaI GTAC 5 cut(s) 265, 913, 1265, 1750, 1931
RsaNI GTAC 5 cut(s) 264, 912, 1264, 1749, 1930
SacI GAGCTC 2 cut(s) 664, 2532
SaqAI TTAA 6 cut(s) 375, 441, 837, 1481, 1599, 1608
Sau96I GGNCC 6 cut(s) 194, 429, 682, 1378, 1744, 2748
SchI GAGTC 5 cut(s) 149, 717, 1265, 1844, 2481
ScrFI CCNGG 4 cut(s) 1257, 1722, 1755, 2630
SduI GDGCHC 4 cut(s) 664, 2232, 2532, 2660
SfaNI GCATC 5 cut(s) 802, 887, 999, 1691, 1705
SfcI CTRYAG 7 cut(s) 121, 1017, 1176, 1197, 1405, 1895, 2651
SfoI GGCGCC 1 cut(s) 1940
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 3 cut(s) 194, 1378, 2748
SmlI CTYRAG 6 cut(s) 440, 727, 836, 1832, 2100, 2498
SmoI CTYRAG 6 cut(s) 440, 727, 836, 1832, 2100, 2498
SpeI ACTAGT 1 cut(s) 271
SsiI CCGC 7 cut(s) 197, 380, 943, 1140, 1419, 1936, 2105
SspDI GGCGCC 1 cut(s) 1938
SspMI CTAG 6 cut(s) 272, 510, 644, 1902, 1949, 2558
SstI GAGCTC 2 cut(s) 664, 2532
StyD4I CCNGG 4 cut(s) 1255, 1720, 1753, 2628
StyI CCWWGG 5 cut(s) 334, 619, 2304, 2557, 2744
TaiI ACGT 4 cut(s) 1673, 2303, 2392, 2512
TaqI TCGA 5 cut(s) 283, 1090, 1643, 2064, 2624
TatI WGTACW 1 cut(s) 1263
TauI GCSGC 2 cut(s) 1421, 1939
Tru1I TTAA 6 cut(s) 375, 441, 837, 1481, 1599, 1608
Tru9I TTAA 6 cut(s) 375, 441, 837, 1481, 1599, 1608
TscAI CASTG 7 cut(s) 322, 370, 876, 1414, 1492, 1745, 1800
TseFI GTSAC 5 cut(s) 113, 287, 1106, 1251, 2162
Tsp45I GTSAC 5 cut(s) 113, 287, 1106, 1251, 2162
TspDTI ATGAA 9 cut(s) 105, 597, 1061, 1239, 1329, 1404, 1653, 2082, 2258
TspGWI ACGGA 5 cut(s) 1379, 1930, 2187, 2374, 2706
TspRI CASTG 7 cut(s) 322, 370, 876, 1414, 1492, 1745, 1800
Tth111I GACNNNGTC 1 cut(s) 2511
Van91I CCANNNNNTGG 2 cut(s) 526, 2539
Vha464I CTTAAG 2 cut(s) 440, 836
VpaK11BI GGWCC 3 cut(s) 194, 1378, 2748
XapI RAATTY 3 cut(s) 765, 1660, 2071
XbaI TCTAGA 1 cut(s) 643
XceI RCATGY 1 cut(s) 2032
XmaJI CCTAGG 1 cut(s) 2557
XspI CTAG 6 cut(s) 272, 510, 644, 1902, 1949, 2558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.