MD15G1383100.v1.1

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007329
Physical Location & Seq
Forward (+)
47191320 .. 47201251
9932 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1383100.v1.1.491

Sequence Viewer

Length: 2760 bp
ATGACTAACCGAATCACCGATGCGCCTCAGGGAGAAGGCGATGCTCCTCCTCCTCCTCCTCCTCCCCCATCTGGTGGCCCTGGTCCACCTGAATCTCAGCCTTCTCGTGGTGGCAACACGATTTTCAAGAGTGGGCCGCTGTTTATATCATCCAAAGGAATTGGATGGACGTCCTGGAAGAAAAGATGGTTTATCTTAACCCGCACTTCACTCGTTTTCTTCAGAAGTGATCCAAGTGCTGCTCTAAAGGGAAGTGAAGTGAATTTGACCCTGGGTGGTATTGACCTCAACAATTCAGGCAGTGTGGTTGTCAAAGCAGACAAGAAACTCTTGACCGTGCTCTTTCCAGATGGCCGTGATGGACGAGCCTTCACACTTAAGGCTGAAACTTTGGAGGATCTACATGAGTGGAAGACTGCACTTGAAAATGCGTTGTCACAATCCCCAAGTGGTGGCGCTCATGCTATGGGGCAAAATGGAATCTTAGGGAATGATAAGACCGAGGCAGTTGATGGTTCTGCGGATCAGTCGAAGGATAAGAAGCCAGTGAAATCTACAGTCATTGGAGTGCCTGTTCTGCTGGCCTTGGAAGAAGTTGATGGAGCTCCGTCTTTCTTGGAAAAAGCCCTTAGGTTTGTAGAAGAATATGGAGTCAAAGTAGAAGGGATCTTACGACAAGCTGCAGATGTTGAAGATGTGGAGAGTCGAATAAGAGAGTATGAACAGGGGAAAACAGAGTTTTCTTCTGAGGAGGATGCGCATGTTATTGCTGATTGTGTCAAGTATGTCCTCCGGGCGTTGCCATCTTCCCCGGTCCCTGCATCTTGTTGTAACGCACTACTAGATGCCTTTCGTAAATCTGGTAATGATCGTGGTGGTAGAGTCAATGCTATGCGCACAACAATATGTGATACGTTCCCGGAGCCAAATCGCCATTTATTGCAAAGAATTCTTTTGATGATGCAAAGTGTGGCTGCCCAGAAAGCTGTGAACCGGATGAGCTGCTCAGCTGTTGCAGCTTGTATGGCTCCCTTACTTCTTCGTCCACTCTTAGCTGGGGATTGTGAAGTTGACAATGATATTGATGTGGGGGGTGATGGTTCTGTTCAACTATTGCAAGCAGCTGCTGCCGCTAATCATGCTCAAGCAATTGTTATAACTTTGTTGGAGGAGTATGATCACATATTTGGGGAAGGTGGTCCCCTGTCCCAGGAACTATACAGTGACACAGAAGAGAGTGAAAGTGAGACCGAGGGGGCAACCGATGATGACGATTACTATGAAGATGATGAAACTGATGCAGAAACAGAGGATGAAGATGTTGAAATTGTTTCTGATGGAGATAGAACGTGCAGTGAGAGTGGTCAATCTGAAGACAAGGATCGGCATGATGATAAGGATGATGATGGTTTAAGACTAGGTGTGAAGACTCCTCATGGAGGCAATGATGGCAAAGCTCACGAGAAATTGTCATCAAGCTCAATTAAAGTTTTGCAGCCTCAACATGCTGTAGAGAGCAGTAAGAATGAACTAAGCTTAAGTAAAACCAATGCGCTAGAGCAGGCTAATGAGTCATCTGTAGTAGAAGAGGTTTCTAGAGAAATAAGTTCACCGAACCAGCCACCCGTTCCATCCCCATCCATCCAAAAATCTTCAACTATATCCAATGGACCTGAGCATGGCAGGAATCGTACTGATTGGGGACGTACCGCTGCAAAGAAGAACCTTTCCATGGAGTCCATTGACTACAGCCTTGAGGAAGAGGCTGAAATCCAGAACCTCGAGATGACTAAATCTGAGTTAGAAAACAGAATTGCCGAAGAGGCTGAAGCGAATGCTGCTCTACAAGCAAGTCTGGAGAGACAGAAGATGGCCTTGCATGAGCGTCGCCTAGCTCTTGAGCAAGATGTGGCAAGACTACAGGAACAGTTACTGAAGGAGAAGGATTTAACAGCAGCTCTTGAAGCAGGACTAAAAGGAAAGGGGGGACGTGTGCCCAATTTAGCCACAGTTGATGAAAAAACAAGGGCAGATCTTACTGAAATAGCTCAGGCAGAGGAGAATGTTGCCAACTTGAACAAGAAGGTTGATGATCTTGGCGTGCAGCTTAATCAACAGTGTGATCGAAACCATGGCTCTATGGCTGATCCTTCCATTTCATTCAGAAATAGTCGCGATCATCTAGCAAAACTGCACACGACAGGTAGACAGGACAGTGAAACCATAGTCCCTTCACATTCAGAATCGTCAAGAAGCAAGGATACTCATCAAGATGGAACAGAAAATGTGAATGAAAAGAAGATAGATGCAGCAAACAAAAGCAACTCCCGGTCCGTTGGGCTGCCAGCTGATTCATCTGCAGTAGAGCCAGTGGTAGCGAAGCCTACTGCTTCAACGAACTCCAAGAAAGGTGGTGGGGGTGAGGGAGGTAGTTCCACAACTTCAGCAATAACGAAGTTGACATCGCGACTGAATTTTTTGAAGGAGCGGCGGAGCCAAATAGCAAATGAGCTTCAAGGCCGTGGCCCTGCTCAAAATTTGGATAAAAGCCAGTCGCTCCAATACACATCAGAGAAACCGGAGAAACTTAGGAAAGCGGAGAGTCATTCAGAGAGAGGAGGAAAGAGATCTGAAAGCCAACAGCAGCAGCATAGCCTGGACAGAGGAAAATCGGAGAGCCATTTGTCTGTTACCGTGGAGAAAGGTCGAGTTGTAGAGAGTAGTAAACAGAAAACTTCTCCGAGGACGGACGCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

920

Amino Acids

99.36

Weight (kDa)

5.07

Isoelectric Point (pI)

53.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 43 - 145 1.1e-11 PH domain
RhoGAP PF00620 203 - 349 2e-28 RhoGAP domain
Lzipper-MIP1 PF14389 626 - 706 3.8e-18 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
AatII GACGTC 1 cut(s) 173
Acc16I TGCGCA 2 cut(s) 759, 896
AccB7I CCANNNNNTGG 2 cut(s) 74, 452
AccBSI CCGCTC 1 cut(s) 2488
AccI GTMKAC 1 cut(s) 2206
AccII CGCG 2 cut(s) 2175, 2467
AciI CCGC 8 cut(s) 137, 202, 521, 1131, 1710, 2488, 2491, 2597
AclWI GGATC 6 cut(s) 224, 405, 531, 674, 1389, 2141
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 4 cut(s) 262, 948, 2473, 2536
AcuI CTGAAG 5 cut(s) 205, 1392, 1848, 1955, 2427
AcyI GRCGYC 2 cut(s) 170, 2751
AfaI GTAC 2 cut(s) 1693, 1708
AfiI CCNNNNNNNGG 8 cut(s) 71, 74, 107, 452, 1210, 1439, 1679, 1732
AflII CTTAAG 2 cut(s) 377, 1537
AflIII ACRYGT 1 cut(s) 1990
AjiI CACGTC 1 cut(s) 1991
AjnI CCWGG 5 cut(s) 79, 173, 270, 1209, 2655
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
Alw21I GWGCWC 2 cut(s) 342, 607
Alw26I GTCTC 2 cut(s) 1241, 1855
AlwI GGATC 6 cut(s) 224, 405, 531, 674, 1389, 2141
AlwNI CAGNNNCTG 2 cut(s) 1127, 1933
Ama87I CYCGRG 1 cut(s) 1781
AoxI GGCC 7 cut(s) 76, 134, 352, 582, 1872, 2518, 2524
ApoI RAATTY 4 cut(s) 262, 948, 2473, 2536
AspLEI GCGC 5 cut(s) 25, 458, 760, 897, 1555
AspS9I GGNCC 8 cut(s) 77, 83, 134, 814, 1199, 1670, 2331, 2525
AsuC2I CCSGG 4 cut(s) 794, 812, 920, 2329
AsuHPI GGTGA 4 cut(s) 7, 1106, 1602, 2432
AvaI CYCGRG 1 cut(s) 1781
AvaII GGWCC 5 cut(s) 83, 814, 1199, 1670, 2331
AxyI CCTNAGG 2 cut(s) 27, 629
BaeGI GKGCMC 1 cut(s) 1998
BanII GRGCYC 1 cut(s) 607
BarI GAAGNNNNNNTAC 4 cut(s) 654, 686, 2245, 2277
BauI CACGAG 2 cut(s) 105, 1460
BbsI GAAGAC 3 cut(s) 419, 1380, 1433
Bbv12I GWGCWC 2 cut(s) 342, 607
BceAI ACGGC 2 cut(s) 339, 2505
BcgI CGANNNNNNTGC 4 cut(s) 193, 227, 1868, 1902
BciT130I CCWGG 5 cut(s) 81, 175, 272, 1211, 2657
BciVI GTATCC 1 cut(s) 2254
BclI TGATCA 1 cut(s) 1177
BcnI CCSGG 4 cut(s) 794, 812, 920, 2329
BcoDI GTCTC 2 cut(s) 1241, 1855
BfaI CTAG 6 cut(s) 842, 1418, 1556, 1596, 1892, 2183
BfmI CTRYAG 7 cut(s) 555, 681, 1509, 1578, 1747, 1919, 2358
BfoI RGCGCY 1 cut(s) 459
BfrI CTTAAG 2 cut(s) 377, 1537
BfuI GTATCC 1 cut(s) 2254
BglI GCCNNNNNGGC 1 cut(s) 1823
BglII AGATCT 2 cut(s) 2032, 2627
BlpI GCTNAGC 1 cut(s) 1006
Bme1390I CCNGG 9 cut(s) 81, 175, 272, 794, 812, 920, 1211, 2329, 2657
Bme18I GGWCC 5 cut(s) 83, 814, 1199, 1670, 2331
BmeT110I CYCGRG 1 cut(s) 1781
BmgBI CACGTC 1 cut(s) 1991
BmgT120I GGNCC 8 cut(s) 77, 83, 134, 814, 1199, 1670, 2331, 2525
BmiI GGNNCC 5 cut(s) 816, 924, 1029, 1201, 2495
BmrFI CCNGG 9 cut(s) 81, 175, 272, 794, 812, 920, 1211, 2329, 2657
BmsI GCATC 8 cut(s) 10, 31, 745, 830, 835, 951, 1288, 2296
BpiI GAAGAC 3 cut(s) 419, 1380, 1433
BpmI CTGGAG 1 cut(s) 1877
Bpu10I CCTNAGC 2 cut(s) 1674, 2049
Bpu1102I GCTNAGC 1 cut(s) 1006
BpuEI CTTGAG 3 cut(s) 1128, 1775, 1919
BpuMI CCSGG 4 cut(s) 794, 812, 920, 2329
BsaBI GATNNNNATC 1 cut(s) 2265
BsaHI GRCGYC 2 cut(s) 170, 2751
BsaI GGTCTC 1 cut(s) 1241
BsaWI WCCGGW 2 cut(s) 993, 2578
BsaXI ACNNNNNCTCC 4 cut(s) 558, 588, 642, 672
Bsc4I CCNNNNNNNGG 8 cut(s) 71, 74, 107, 452, 1210, 1439, 1679, 1732
Bse1I ACTGG 3 cut(s) 545, 2369, 2551
Bse21I CCTNAGG 2 cut(s) 27, 629
Bse3DI GCAATG 1 cut(s) 1450
Bse8I GATNNNNATC 1 cut(s) 2265
BseBI CCWGG 5 cut(s) 81, 175, 272, 1211, 2657
BseGI GGATG 9 cut(s) 149, 170, 760, 1002, 1318, 1405, 1631, 1637, 1641
BseJI GATNNNNATC 1 cut(s) 2265
BseLI CCNNNNNNNGG 8 cut(s) 71, 74, 107, 452, 1210, 1439, 1679, 1732
BseMI GCAATG 1 cut(s) 1450
BseMII CTCAG 7 cut(s) 41, 110, 738, 1020, 1665, 1788, 2063
BseNI ACTGG 3 cut(s) 545, 2369, 2551
BseSI GKGCMC 1 cut(s) 1998
BseYI CCCAGC 1 cut(s) 1055
BsgI GTGCAG 4 cut(s) 402, 1372, 2123, 2177
Bsh1236I CGCG 2 cut(s) 2175, 2467
BshFI GGCC 7 cut(s) 78, 136, 354, 584, 1874, 2520, 2526
BsiHKAI GWGCWC 2 cut(s) 342, 607
BsiHKCI CYCGRG 1 cut(s) 1781
BsiSI CCGG 6 cut(s) 793, 812, 920, 994, 2329, 2579
BslFI GGGAC 6 cut(s) 800, 1185, 1192, 1716, 2001, 2213
BslI CCNNNNNNNGG 8 cut(s) 71, 74, 107, 452, 1210, 1439, 1679, 1732
BsmAI GTCTC 2 cut(s) 1241, 1855
BsmFI GGGAC 6 cut(s) 800, 1185, 1192, 1716, 2001, 2213
BsmI GAATGC 1 cut(s) 1840
BsnI GGCC 7 cut(s) 78, 136, 354, 584, 1874, 2520, 2526
Bso31I GGTCTC 1 cut(s) 1241
BsoBI CYCGRG 1 cut(s) 1781
Bsp1286I GDGCHC 3 cut(s) 342, 607, 1998
Bsp1720I GCTNAGC 1 cut(s) 1006
Bsp19I CCATGG 2 cut(s) 1731, 2131
Bsp68I TCGCGA 2 cut(s) 2175, 2467
BspACI CCGC 8 cut(s) 137, 202, 521, 1131, 1710, 2488, 2491, 2597
BspANI GGCC 7 cut(s) 78, 136, 354, 584, 1874, 2520, 2526
BspCNI CTCAG 7 cut(s) 40, 109, 739, 1019, 1666, 1789, 2062
BspFNI CGCG 2 cut(s) 2175, 2467
BspLI GGNNCC 5 cut(s) 816, 924, 1029, 1201, 2495
BspMAI CTGCAG 2 cut(s) 685, 2362
BspPI GGATC 6 cut(s) 224, 405, 531, 674, 1389, 2141
BspTI CTTAAG 2 cut(s) 377, 1537
BspTNI GGTCTC 1 cut(s) 1241
BsrBI CCGCTC 1 cut(s) 2488
BsrDI GCAATG 1 cut(s) 1450
BsrI ACTGG 3 cut(s) 545, 2369, 2551
BssNI GRCGYC 2 cut(s) 170, 2751
BssSI CACGAG 2 cut(s) 105, 1460
BssT1I CCWWGG 3 cut(s) 585, 1731, 2131
Bst2BI CACGAG 2 cut(s) 105, 1460
Bst2UI CCWGG 5 cut(s) 81, 175, 272, 1211, 2657
Bst4CI ACNGT 8 cut(s) 337, 559, 1223, 1929, 2011, 2118, 2216, 2695
Bst6I CTCTTC 4 cut(s) 1227, 1581, 1755, 1815
BstACI GRCGYC 2 cut(s) 170, 2751
BstAFI CTTAAG 2 cut(s) 377, 1537
BstAPI GCANNNNNTGC 1 cut(s) 1127
BstC8I GCNNGC 5 cut(s) 582, 1119, 1563, 2102, 2346
BstDSI CCRYGG 4 cut(s) 1731, 2131, 2521, 2694
BstENI CCTNNNNNAGG 2 cut(s) 1208, 1437
BstF5I GGATG 9 cut(s) 149, 170, 760, 1002, 1318, 1405, 1631, 1637, 1641
BstFNI CGCG 2 cut(s) 2175, 2467
BstH2I RGCGCY 1 cut(s) 459
BstHHI GCGC 5 cut(s) 25, 458, 760, 897, 1555
BstMAI GTCTC 2 cut(s) 1241, 1855
BstNI CCWGG 5 cut(s) 81, 175, 272, 1211, 2657
BstNSI RCATGY 2 cut(s) 764, 1508
BstSCI CCNGG 9 cut(s) 79, 173, 270, 792, 810, 918, 1209, 2327, 2655
BstSFI CTRYAG 7 cut(s) 555, 681, 1509, 1578, 1747, 1919, 2358
BstSLI GKGCMC 1 cut(s) 1998
BstUI CGCG 2 cut(s) 2175, 2467
BstV2I GAAGAC 3 cut(s) 419, 1380, 1433
BstX2I RGATCY 4 cut(s) 397, 666, 2032, 2627
BstYI RGATCY 4 cut(s) 397, 666, 2032, 2627
Bsu36I CCTNAGG 2 cut(s) 27, 629
BsuI GTATCC 1 cut(s) 2254
BsuRI GGCC 7 cut(s) 78, 136, 354, 584, 1874, 2520, 2526
BtgI CCRYGG 4 cut(s) 1731, 2131, 2521, 2694
BtgZI GCGATG 2 cut(s) 54, 2448
BtrI CACGTC 1 cut(s) 1991
BtsCI GGATG 9 cut(s) 149, 170, 760, 1002, 1318, 1405, 1631, 1637, 1641
BtsI GCAGTG 2 cut(s) 307, 1360
BtsIMutI CAGTG 7 cut(s) 307, 552, 1228, 1360, 2123, 2221, 2376
BtuMI TCGCGA 2 cut(s) 2175, 2467
Cac8I GCNNGC 5 cut(s) 582, 1119, 1563, 2102, 2346
CaiI CAGNNNCTG 2 cut(s) 1127, 1933
CfoI GCGC 5 cut(s) 25, 458, 760, 897, 1555
Cfr13I GGNCC 8 cut(s) 77, 83, 134, 814, 1199, 1670, 2331, 2525
CpoI CGGWCCG 1 cut(s) 2331
CseI GACGC 1 cut(s) 1874
Csp6I GTAC 2 cut(s) 1692, 1707
CspCI CAANNNNNGTGG 2 cut(s) 2392, 2427
CspI CGGWCCG 1 cut(s) 2331
CviQI GTAC 2 cut(s) 1692, 1707
EaeI YGGCCR 1 cut(s) 352
Eam1104I CTCTTC 4 cut(s) 1227, 1581, 1755, 1815
EarI CTCTTC 4 cut(s) 1227, 1581, 1755, 1815
EciI GGCGGA 1 cut(s) 2506
Ecl136II GAGCTC 1 cut(s) 605
Eco130I CCWWGG 3 cut(s) 585, 1731, 2131
Eco24I GRGCYC 1 cut(s) 607
Eco31I GGTCTC 1 cut(s) 1241
Eco47I GGWCC 5 cut(s) 83, 814, 1199, 1670, 2331
Eco53kI GAGCTC 1 cut(s) 605
Eco57I CTGAAG 5 cut(s) 205, 1392, 1848, 1955, 2427
Eco81I CCTNAGG 2 cut(s) 27, 629
Eco88I CYCGRG 1 cut(s) 1781
EcoICRI GAGCTC 1 cut(s) 605
EcoNI CCTNNNNNAGG 2 cut(s) 1208, 1437
EcoRI GAATTC 1 cut(s) 948
EcoRII CCWGG 5 cut(s) 79, 173, 270, 1209, 2655
EcoT14I CCWWGG 3 cut(s) 585, 1731, 2131
EcoT38I GRGCYC 1 cut(s) 607
ErhI CCWWGG 3 cut(s) 585, 1731, 2131
FalI AAGNNNNNCTT 4 cut(s) 314, 346, 1859, 1891
FaqI GGGAC 6 cut(s) 800, 1185, 1192, 1716, 2001, 2213
FauI CCCGC 1 cut(s) 209
FbaI TGATCA 1 cut(s) 1177
FblI GTMKAC 1 cut(s) 2206
FokI GGATG 9 cut(s) 136, 177, 767, 1009, 1325, 1412, 1618, 1624, 1628
FriOI GRGCYC 1 cut(s) 607
FspAI RTGCGCAY 2 cut(s) 759, 896
FspBI CTAG 6 cut(s) 842, 1418, 1556, 1596, 1892, 2183
FspI TGCGCA 2 cut(s) 759, 896
GlaI GCGC 5 cut(s) 24, 457, 759, 896, 1554
GsaI CCCAGC 1 cut(s) 1059
GsuI CTGGAG 1 cut(s) 1877
HaeII RGCGCY 1 cut(s) 459
HaeIII GGCC 7 cut(s) 78, 136, 354, 584, 1874, 2520, 2526
HapII CCGG 6 cut(s) 793, 812, 920, 994, 2329, 2579
HgaI GACGC 1 cut(s) 1874
HhaI GCGC 5 cut(s) 25, 458, 760, 897, 1555
Hin1I GRCGYC 2 cut(s) 170, 2751
Hin6I GCGC 5 cut(s) 23, 456, 758, 895, 1553
HinP1I GCGC 5 cut(s) 23, 456, 758, 895, 1553
HincII GTYRAC 2 cut(s) 1072, 2460
HindII GTYRAC 2 cut(s) 1072, 2460
HindIII AAGCTT 1 cut(s) 1534
HpaII CCGG 6 cut(s) 793, 812, 920, 994, 2329, 2579
HphI GGTGA 4 cut(s) 7, 1106, 1602, 2432
Hpy166II GTNNAC 8 cut(s) 86, 991, 1046, 1072, 1610, 2207, 2460, 2726
Hpy8I GTNNAC 8 cut(s) 86, 991, 1046, 1072, 1610, 2207, 2460, 2726
Hpy99I CGWCG 1 cut(s) 1890
HpyCH4III ACNGT 8 cut(s) 337, 559, 1223, 1929, 2011, 2118, 2216, 2695
HpyCH4IV ACGT 5 cut(s) 170, 914, 1349, 1705, 1990
HpySE526I ACGT 5 cut(s) 170, 914, 1349, 1705, 1990
Hsp92I GRCGYC 2 cut(s) 170, 2751
HspAI GCGC 5 cut(s) 23, 456, 758, 895, 1553
Ksp22I TGATCA 1 cut(s) 1177
LmnI GCTCC 8 cut(s) 49, 602, 610, 922, 1033, 2485, 2493, 2562
LweI GCATC 8 cut(s) 10, 31, 745, 830, 835, 951, 1288, 2296
MaeI CTAG 6 cut(s) 842, 1418, 1556, 1596, 1892, 2183
MaeII ACGT 5 cut(s) 170, 914, 1349, 1705, 1990
MaeIII GTNAC 5 cut(s) 435, 830, 1223, 1929, 2689
MbiI CCGCTC 1 cut(s) 2488
MfeI CAATTG 1 cut(s) 1149
MflI RGATCY 4 cut(s) 397, 666, 2032, 2627
MhlI GDGCHC 3 cut(s) 342, 607, 1998
MlyI GAGTC 7 cut(s) 660, 712, 891, 1423, 1580, 1745, 2611
MmeI TCCRAC 1 cut(s) 1146
MseI TTAA 7 cut(s) 197, 378, 1412, 1485, 1538, 1949, 2109
MslI CAYNNNNRTG 2 cut(s) 566, 2271
MspA1I CMGCKG 5 cut(s) 139, 1010, 1124, 1712, 2348
MspCI CTTAAG 2 cut(s) 377, 1537
MspI CCGG 6 cut(s) 793, 812, 920, 994, 2329, 2579
MspR9I CCNGG 9 cut(s) 81, 175, 272, 794, 812, 920, 1211, 2329, 2657
MunI CAATTG 1 cut(s) 1149
Mva1269I GAATGC 1 cut(s) 1840
MvaI CCWGG 5 cut(s) 81, 175, 272, 1211, 2657
MvnI CGCG 2 cut(s) 2175, 2467
NciI CCSGG 4 cut(s) 794, 812, 920, 2329
NcoI CCATGG 2 cut(s) 1731, 2131
NlaIV GGNNCC 5 cut(s) 816, 924, 1029, 1201, 2495
NmuCI GTSAC 2 cut(s) 435, 1223
NruI TCGCGA 2 cut(s) 2175, 2467
NsbI TGCGCA 2 cut(s) 759, 896
NspI RCATGY 2 cut(s) 764, 1508
PaeR7I CTCGAG 1 cut(s) 1781
PasI CCCWGGG 1 cut(s) 271
PctI GAATGC 1 cut(s) 1840
PfeI GAWTC 6 cut(s) 12, 92, 480, 1687, 2243, 2351
PflMI CCANNNNNTGG 2 cut(s) 74, 452
PfoI TCCNGGA 2 cut(s) 173, 918
PleI GAGTC 7 cut(s) 659, 711, 890, 1423, 1579, 1744, 2610
PpsI GAGTC 7 cut(s) 659, 711, 890, 1423, 1579, 1744, 2610
PsiI TTATAA 1 cut(s) 1157
Psp124BI GAGCTC 1 cut(s) 607
Psp6I CCWGG 5 cut(s) 79, 173, 270, 1209, 2655
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 5 cut(s) 79, 173, 270, 1209, 2655
PspN4I GGNNCC 5 cut(s) 816, 924, 1029, 1201, 2495
PspPI GGNCC 8 cut(s) 77, 83, 134, 814, 1199, 1670, 2331, 2525
PstI CTGCAG 2 cut(s) 685, 2362
PstNI CAGNNNCTG 2 cut(s) 1127, 1933
PsuI RGATCY 4 cut(s) 397, 666, 2032, 2627
PvuII CAGCTG 3 cut(s) 1010, 1124, 2348
RruI TCGCGA 2 cut(s) 2175, 2467
RsaI GTAC 2 cut(s) 1693, 1708
RsaNI GTAC 2 cut(s) 1692, 1707
RseI CAYNNNNRTG 2 cut(s) 566, 2271
Rsr2I CGGWCCG 1 cut(s) 2331
RsrII CGGWCCG 1 cut(s) 2331
SacI GAGCTC 1 cut(s) 607
SaqAI TTAA 7 cut(s) 197, 378, 1412, 1485, 1538, 1949, 2109
Sau96I GGNCC 8 cut(s) 77, 83, 134, 814, 1199, 1670, 2331, 2525
SchI GAGTC 7 cut(s) 660, 712, 891, 1423, 1580, 1745, 2611
ScrFI CCNGG 9 cut(s) 81, 175, 272, 794, 812, 920, 1211, 2329, 2657
SduI GDGCHC 3 cut(s) 342, 607, 1998
SfaNI GCATC 8 cut(s) 10, 31, 745, 830, 835, 951, 1288, 2296
SfcI CTRYAG 7 cut(s) 555, 681, 1509, 1578, 1747, 1919, 2358
Sfr274I CTCGAG 1 cut(s) 1781
SinI GGWCC 5 cut(s) 83, 814, 1199, 1670, 2331
SlaI CTCGAG 1 cut(s) 1781
SmiMI CAYNNNNRTG 2 cut(s) 566, 2271
SmlI CTYRAG 6 cut(s) 377, 1143, 1537, 1754, 1781, 1898
SmoI CTYRAG 6 cut(s) 377, 1143, 1537, 1754, 1781, 1898
SsiI CCGC 8 cut(s) 137, 202, 521, 1131, 1710, 2488, 2491, 2597
SspMI CTAG 6 cut(s) 842, 1418, 1556, 1596, 1892, 2183
SstI GAGCTC 1 cut(s) 607
StyD4I CCNGG 9 cut(s) 79, 173, 270, 792, 810, 918, 1209, 2327, 2655
StyI CCWWGG 3 cut(s) 585, 1731, 2131
TaaI ACNGT 8 cut(s) 337, 559, 1223, 1929, 2011, 2118, 2216, 2695
TaiI ACGT 5 cut(s) 173, 917, 1352, 1708, 1993
TaqI TCGA 5 cut(s) 530, 706, 1782, 2125, 2707
TaqII GACCGA 2 cut(s) 515, 1265
TauI GCSGC 3 cut(s) 139, 1133, 2491
TfiI GAWTC 6 cut(s) 12, 92, 480, 1687, 2243, 2351
Tru1I TTAA 7 cut(s) 197, 378, 1412, 1485, 1538, 1949, 2109
Tru9I TTAA 7 cut(s) 197, 378, 1412, 1485, 1538, 1949, 2109
TscAI CASTG 7 cut(s) 307, 552, 1228, 1360, 2123, 2221, 2376
TseFI GTSAC 2 cut(s) 435, 1223
Tsp45I GTSAC 2 cut(s) 435, 1223
TspDTI ATGAA 9 cut(s) 735, 1296, 1305, 1329, 1542, 2031, 2148, 2307, 2343
TspGWI ACGGA 2 cut(s) 597, 2323
TspRI CASTG 7 cut(s) 307, 552, 1228, 1360, 2123, 2221, 2376
Van91I CCANNNNNTGG 2 cut(s) 74, 452
Vha464I CTTAAG 2 cut(s) 377, 1537
VpaK11BI GGWCC 5 cut(s) 83, 814, 1199, 1670, 2331
XagI CCTNNNNNAGG 2 cut(s) 1208, 1437
XapI RAATTY 4 cut(s) 262, 948, 2473, 2536
XbaI TCTAGA 1 cut(s) 1595
XceI RCATGY 2 cut(s) 764, 1508
XhoI CTCGAG 1 cut(s) 1781
XmiI GTMKAC 1 cut(s) 2206
XspI CTAG 6 cut(s) 842, 1418, 1556, 1596, 1892, 2183
ZraI GACGTC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.