Rmu_sc0000272.1_g000001

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000272.1
OG0007329
Physical Location & Seq
Forward (+)
1 .. 9787
9787 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000272.1_g000001.1.cds

Sequence Viewer

Length: 2127 bp
ttttatgaatcagggaaagttgatttttctcccgaggaggatccacatgtcattgctgattgtgtcaagtatgtcctccgagagttgccatcttctccagtccctgcatcttgctgtaacgcactgctcgattcctttcgcaagtctggtaatgatcggggtggtagaatcaatgccatgcgtacgacaatatgtgatacattcccagaaccaaaccgtcgtttattgcaaagaattctattgatgatgcaaactattgcttctcacaaagccgtgaatcgaatgagctgctcagccgtggcagcttgcatggctcccttacttcttcgtccactcctggctggggattgtgaaatcgacaatgattttagtgtgggtggcgacaattcggttcagcttttgcaagcagctgctgcagctaatcatgctcaagcaattgttataactttgctggaggagtatgataacatatttggggaaggttccatatccgctgaactatacagtgacacagatgacgatgaaactgaaaccgagggggaaactgaagatgatgagtactatgaagatgatgaaactgagtctggagaatatgatgacgatgatgatgttgaaattgtcagtgggtcatgcagtgagagtgatatagatgatgaacagtttgacaataaggctggttccaaggttcctgatggaggcaatgatagcaaatctccaaagaagttgtcatcaagctcacttaaacatgtgaaacatcaatatagtgtaaaaagcaatgataataaacagaatgtaagtgaaattaactcagcagagctggctaatgagtctactggagtagcaggtgtttctagagaaaaaagttctgtgcatcagtcaactggccatggccttccatgcatgcaaaaatcttcaactatatccaatggacctgattatggtgcaacacatcgtcctaactggggacgtaccggtgcaaggaagaacctttccatggaatccattgactttggtgttgaggaggaggatgaagaaatccagaggctagagattacaaaagctgagttagaaaataaaattgtagaagaggttcaagggaatgtagctctgcaagcaaatctagaaaagcagaagaaggccttgcttgaacgtcgtcgagctctcaaacaagatgtggcaagactacaggaacagttacagaaggagagagatttgggagcagcccttgaagcagggctaaaaatctccgagggccatgtacctaatttagccgatgttgatgaaaaaacaagggcagagcttgaggaaatggttcaggcagaggcggattgtaccaacttgaaaaagaaggttgatgatctaggtgtgcagcttaatcaacaacgtgaacgaaactatggctctagctctgcgtctgatgcatccaatttgtcgcaacatagtcataatacaaaacagcagacgaaggataaacagaaggatagtgaagcagtagtcccttcacattttgaatggtcaagaagtaaggatggtcatcaagatggcgcagaaaatgcaaatgagaggaaggtggatggactacataaactctcccgacttgttggcggtatatccgattcttctgcagtggaaccagttgtttccaagcatggcaagaaagggggtgaggggggtagtacatcttcagcaataacaaagttgacatcgcgactaaactttttgaaggagcggcggagccaaatagctaatgaaattcaaagcagagataaagttcaatgttcccaaaacttggagaaatgtcaatcgctcccagataatccggaaaaagttagaggattggaaggggcgccttccaatcccagaaacctccgccgcccacctccaagatctcaactccaggagtccagaaccggcggccccacccccagaaccggccggaaaaccaccgaaccggccaccggaagtagcaaaagtccctttgcccggttcaaagccttcctcaccacctcctgcaaccatactcgaccacctgcagcgtctccaccccagaaaaccggaaccggaaatttcatcaccagaaccggccaaaattcccccgaaccggcccccggaatcctcctaacctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

708

Amino Acids

77.29

Weight (kDa)

5.22

Isoelectric Point (pI)

54.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 443
AarI CACCTGC 2 cut(s) 833, 2038
Acc36I ACCTGC 2 cut(s) 833, 2038
AccB1I GGYRCC 1 cut(s) 1846
AccB7I CCANNNNNTGG 1 cut(s) 1789
AccBSI CCGCTC 1 cut(s) 1729
AccI GTMKAC 1 cut(s) 830
AccII CGCG 1 cut(s) 1708
AccIII TCCGGA 1 cut(s) 1819
AciI CCGC 8 cut(s) 492, 1325, 1605, 1729, 1732, 1870, 1873, 1914
AclWI GGATC 2 cut(s) 35, 48
AcoI YGGCCR 4 cut(s) 883, 1933, 1953, 2083
AcsI RAATTY 4 cut(s) 234, 1752, 2065, 2089
AcuI CTGAAG 2 cut(s) 567, 1668
AcyI GRCGYC 1 cut(s) 1847
AfaI GTAC 6 cut(s) 184, 560, 970, 1260, 1333, 1678
AflIII ACRYGT 2 cut(s) 46, 745
AgeI ACCGGT 1 cut(s) 971
AjnI CCWGG 2 cut(s) 336, 1896
Alw21I GWGCWC 1 cut(s) 1162
Alw26I GTCTC 1 cut(s) 2043
AlwI GGATC 2 cut(s) 35, 48
AlwNI CAGNNNCTG 2 cut(s) 104, 413
Ama87I CYCGRG 1 cut(s) 32
Aor13HI TCCGGA 1 cut(s) 1819
AoxI GGCC 9 cut(s) 883, 889, 1137, 1252, 1915, 1933, 1953, 2083, 2103
ApeKI GCWGC 9 cut(s) 288, 302, 407, 410, 413, 416, 1220, 1369, 2033
ApoI RAATTY 4 cut(s) 234, 1752, 2065, 2089
ArsI GACNNNNNNTTYG 2 cut(s) 710, 742
AsiGI ACCGGT 1 cut(s) 971
Asp700I GAANNNNTTC 2 cut(s) 1089, 1311
AspLEI GCGC 2 cut(s) 1547, 1849
AspS9I GGNCC 4 cut(s) 929, 1252, 1916, 2104
AsuC2I CCSGG 2 cut(s) 1984, 2109
AsuHPI GGTGA 3 cut(s) 1676, 1993, 2065
AvaI CYCGRG 1 cut(s) 32
AvaII GGWCC 1 cut(s) 929
BalI TGGCCA 1 cut(s) 885
BamHI GGATCC 1 cut(s) 40
BanI GGYRCC 1 cut(s) 1846
BanII GRGCYC 1 cut(s) 1162
BarI GAAGNNNNNNTAC 2 cut(s) 1666, 1698
Bbv12I GWGCWC 1 cut(s) 1162
BbvI GCAGC 9 cut(s) 275, 314, 397, 400, 419, 428, 1232, 1381, 2045
BccI CCATC 5 cut(s) 97, 686, 1523, 1535, 1568
BceAI ACGGC 2 cut(s) 257, 281
BcgI CGANNNNNNTGC 4 cut(s) 270, 304, 1604, 1638
BciT130I CCWGG 2 cut(s) 338, 1898
BcnI CCSGG 2 cut(s) 1984, 2109
BcoDI GTCTC 1 cut(s) 2043
BfaI CTAG 5 cut(s) 852, 1046, 1121, 1361, 1404
BfmI CTRYAG 4 cut(s) 414, 1184, 1623, 2031
BfoI RGCGCY 1 cut(s) 1850
BfuAI ACCTGC 2 cut(s) 833, 2038
BglII AGATCT 1 cut(s) 1886
BlpI GCTNAGC 1 cut(s) 292
BmcAI AGTACT 1 cut(s) 560
Bme1390I CCNGG 4 cut(s) 338, 1898, 1984, 2109
Bme18I GGWCC 1 cut(s) 929
BmeT110I CYCGRG 1 cut(s) 32
BmgT120I GGNCC 4 cut(s) 929, 1252, 1916, 2104
BmrFI CCNGG 4 cut(s) 338, 1898, 1984, 2109
BmrI ACTGGG 1 cut(s) 970
BmsI GCATC 5 cut(s) 116, 237, 880, 1408, 1430
BmuI ACTGGG 1 cut(s) 970
BpmI CTGGAG 5 cut(s) 81, 473, 606, 855, 1880
Bpu1102I GCTNAGC 1 cut(s) 292
BpuEI CTTGAG 2 cut(s) 414, 1322
BpuMI CCSGG 2 cut(s) 1984, 2109
BsaBI GATNNNNATC 1 cut(s) 1533
BsaHI GRCGYC 1 cut(s) 1847
BsaJI CCNNGG 8 cut(s) 33, 297, 534, 681, 886, 993, 1248, 2107
BsaWI WCCGGW 5 cut(s) 971, 1819, 1958, 2054, 2060
Bse118I RCCGGY 6 cut(s) 971, 1910, 1931, 1951, 2081, 2101
Bse1I ACTGG 5 cut(s) 98, 838, 886, 965, 1634
Bse3DI GCAATG 3 cut(s) 51, 706, 781
Bse8I GATNNNNATC 1 cut(s) 1533
BseAI TCCGGA 1 cut(s) 1819
BseBI CCWGG 2 cut(s) 338, 1898
BseDI CCNNGG 8 cut(s) 33, 297, 534, 681, 886, 993, 1248, 2107
BseGI GGATG 4 cut(s) 1033, 1421, 1534, 1579
BseJI GATNNNNATC 1 cut(s) 1533
BseMI GCAATG 3 cut(s) 51, 706, 781
BseMII CTCAG 4 cut(s) 306, 570, 822, 1053
BseNI ACTGG 5 cut(s) 98, 838, 886, 965, 1634
BseRI GAGGAG 4 cut(s) 50, 470, 1034, 1037
BseX3I CGGCCG 1 cut(s) 1933
BseXI GCAGC 9 cut(s) 275, 314, 397, 400, 419, 428, 1232, 1381, 2045
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 1388
Bsh1236I CGCG 1 cut(s) 1708
Bsh1285I CGRYCG 1 cut(s) 1936
BshFI GGCC 9 cut(s) 885, 891, 1139, 1254, 1917, 1935, 1955, 2085, 2105
BshNI GGYRCC 1 cut(s) 1846
BshTI ACCGGT 1 cut(s) 971
BsiEI CGRYCG 1 cut(s) 1936
BsiHKAI GWGCWC 1 cut(s) 1162
BsiHKCI CYCGRG 1 cut(s) 32
BsiWI CGTACG 1 cut(s) 182
BslFI GGGAC 4 cut(s) 86, 978, 1481, 1959
BsmAI GTCTC 1 cut(s) 2043
BsmBI CGTCTC 1 cut(s) 2043
BsmFI GGGAC 4 cut(s) 86, 978, 1481, 1959
BsnI GGCC 9 cut(s) 885, 891, 1139, 1254, 1917, 1935, 1955, 2085, 2105
BsoBI CYCGRG 1 cut(s) 32
Bsp1286I GDGCHC 1 cut(s) 1162
Bsp13I TCCGGA 1 cut(s) 1819
Bsp143I GATC 4 cut(s) 40, 154, 1357, 1886
Bsp1720I GCTNAGC 1 cut(s) 292
Bsp19I CCATGG 2 cut(s) 886, 993
Bsp68I TCGCGA 1 cut(s) 1708
BspACI CCGC 8 cut(s) 492, 1325, 1605, 1729, 1732, 1870, 1873, 1914
BspANI GGCC 9 cut(s) 885, 891, 1139, 1254, 1917, 1935, 1955, 2085, 2105
BspCNI CTCAG 4 cut(s) 305, 571, 821, 1054
BspEI TCCGGA 1 cut(s) 1819
BspFNI CGCG 1 cut(s) 1708
BspMAI CTGCAG 3 cut(s) 418, 1627, 2035
BspMI ACCTGC 2 cut(s) 833, 2038
BspPI GGATC 2 cut(s) 35, 48
BspT107I GGYRCC 1 cut(s) 1846
BsrBI CCGCTC 1 cut(s) 1729
BsrDI GCAATG 3 cut(s) 51, 706, 781
BsrFI RCCGGY 6 cut(s) 971, 1910, 1931, 1951, 2081, 2101
BsrI ACTGG 5 cut(s) 98, 838, 886, 965, 1634
BssAI RCCGGY 6 cut(s) 971, 1910, 1931, 1951, 2081, 2101
BssECI CCNNGG 8 cut(s) 33, 297, 534, 681, 886, 993, 1248, 2107
BssMI GATC 4 cut(s) 40, 154, 1357, 1886
BssNI GRCGYC 1 cut(s) 1847
BssT1I CCWWGG 3 cut(s) 681, 886, 993
Bst2UI CCWGG 2 cut(s) 338, 1898
Bst4CI ACNGT 4 cut(s) 218, 506, 660, 1194
Bst6I CTCTTC 1 cut(s) 1080
BstACI GRCGYC 1 cut(s) 1847
BstAPI GCANNNNNTGC 2 cut(s) 413, 1553
BstC8I GCNNGC 5 cut(s) 307, 405, 819, 902, 1113
BstDEI CTNAG 4 cut(s) 292, 579, 808, 1062
BstDSI CCRYGG 3 cut(s) 297, 886, 993
BstENI CCTNNNNNAGG 1 cut(s) 693
BstF5I GGATG 4 cut(s) 1033, 1421, 1534, 1579
BstFNI CGCG 1 cut(s) 1708
BstH2I RGCGCY 1 cut(s) 1850
BstHHI GCGC 2 cut(s) 1547, 1849
BstKTI GATC 4 cut(s) 43, 157, 1360, 1889
BstMAI GTCTC 1 cut(s) 2043
BstMBI GATC 4 cut(s) 40, 154, 1357, 1886
BstMCI CGRYCG 1 cut(s) 1936
BstNI CCWGG 2 cut(s) 338, 1898
BstNSI RCATGY 3 cut(s) 50, 749, 904
BstSCI CCNGG 4 cut(s) 336, 1896, 1982, 2107
BstSFI CTRYAG 4 cut(s) 414, 1184, 1623, 2031
BstUI CGCG 1 cut(s) 1708
BstV1I GCAGC 9 cut(s) 275, 314, 397, 400, 419, 428, 1232, 1381, 2045
BstX2I RGATCY 2 cut(s) 40, 1886
BstYI RGATCY 2 cut(s) 40, 1886
BstZI CGGCCG 1 cut(s) 1933
BsuRI GGCC 9 cut(s) 885, 891, 1139, 1254, 1917, 1935, 1955, 2085, 2105
BtgI CCRYGG 3 cut(s) 297, 886, 993
BtgZI GCGATG 1 cut(s) 1689
BtsCI GGATG 4 cut(s) 1033, 1421, 1534, 1579
BtsI GCAGTG 3 cut(s) 122, 640, 1632
BtsIMutI CAGTG 5 cut(s) 122, 511, 628, 640, 1632
BtuMI TCGCGA 1 cut(s) 1708
BveI ACCTGC 2 cut(s) 833, 2038
Cac8I GCNNGC 5 cut(s) 307, 405, 819, 902, 1113
CaiI CAGNNNCTG 2 cut(s) 104, 413
CfoI GCGC 2 cut(s) 1547, 1849
Cfr10I RCCGGY 6 cut(s) 971, 1910, 1931, 1951, 2081, 2101
Cfr13I GGNCC 4 cut(s) 929, 1252, 1916, 2104
CseI GACGC 2 cut(s) 1401, 2025
Csp6I GTAC 6 cut(s) 183, 559, 969, 1259, 1332, 1677
CspAI ACCGGT 1 cut(s) 971
CviQI GTAC 6 cut(s) 183, 559, 969, 1259, 1332, 1677
DdeI CTNAG 4 cut(s) 292, 579, 808, 1062
DinI GGCGCC 1 cut(s) 1848
DpnI GATC 4 cut(s) 42, 156, 1359, 1888
DpnII GATC 4 cut(s) 40, 154, 1357, 1886
EaeI YGGCCR 4 cut(s) 883, 1933, 1953, 2083
EagI CGGCCG 1 cut(s) 1933
Eam1104I CTCTTC 1 cut(s) 1080
EarI CTCTTC 1 cut(s) 1080
EciI GGCGGA 3 cut(s) 1340, 1747, 1859
Ecl136II GAGCTC 1 cut(s) 1160
EclXI CGGCCG 1 cut(s) 1933
Eco130I CCWWGG 3 cut(s) 681, 886, 993
Eco147I AGGCCT 1 cut(s) 1139
Eco24I GRGCYC 1 cut(s) 1162
Eco47I GGWCC 1 cut(s) 929
Eco52I CGGCCG 1 cut(s) 1933
Eco53kI GAGCTC 1 cut(s) 1160
Eco57I CTGAAG 2 cut(s) 567, 1668
Eco88I CYCGRG 1 cut(s) 32
EcoICRI GAGCTC 1 cut(s) 1160
EcoNI CCTNNNNNAGG 1 cut(s) 693
EcoRI GAATTC 1 cut(s) 234
EcoRII CCWGG 2 cut(s) 336, 1896
EcoT14I CCWWGG 3 cut(s) 681, 886, 993
EcoT22I ATGCAT 2 cut(s) 902, 1423
EcoT38I GRGCYC 1 cut(s) 1162
EgeI GGCGCC 1 cut(s) 1848
EheI GGCGCC 1 cut(s) 1848
ErhI CCWWGG 3 cut(s) 681, 886, 993
Esp3I CGTCTC 1 cut(s) 2043
FalI AAGNNNNNCTT 2 cut(s) 1124, 1156
FaqI GGGAC 4 cut(s) 86, 978, 1481, 1959
FblI GTMKAC 1 cut(s) 830
FokI GGATG 4 cut(s) 1040, 1408, 1541, 1586
FriOI GRGCYC 1 cut(s) 1162
FspBI CTAG 5 cut(s) 852, 1046, 1121, 1361, 1404
GlaI GCGC 2 cut(s) 1546, 1848
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 5 cut(s) 81, 473, 606, 855, 1880
HaeII RGCGCY 1 cut(s) 1850
HaeIII GGCC 9 cut(s) 885, 891, 1139, 1254, 1917, 1935, 1955, 2085, 2105
HgaI GACGC 2 cut(s) 1401, 2025
HhaI GCGC 2 cut(s) 1547, 1849
Hin1I GRCGYC 1 cut(s) 1847
Hin6I GCGC 2 cut(s) 1545, 1847
HinP1I GCGC 2 cut(s) 1545, 1847
HincII GTYRAC 2 cut(s) 879, 1701
HindII GTYRAC 2 cut(s) 879, 1701
HphI GGTGA 3 cut(s) 1676, 1993, 2065
Hpy166II GTNNAC 5 cut(s) 332, 831, 879, 1388, 1701
Hpy188I TCNGA 4 cut(s) 80, 1249, 1417, 1615
Hpy8I GTNNAC 5 cut(s) 332, 831, 879, 1388, 1701
Hpy99I CGWCG 3 cut(s) 222, 1155, 1158
HpyCH4III ACNGT 4 cut(s) 218, 506, 660, 1194
HpyCH4IV ACGT 3 cut(s) 967, 1150, 1384
HpyF3I CTNAG 4 cut(s) 292, 579, 808, 1062
HpySE526I ACGT 3 cut(s) 967, 1150, 1384
Hsp92I GRCGYC 1 cut(s) 1847
HspAI GCGC 2 cut(s) 1545, 1847
KasI GGCGCC 1 cut(s) 1846
Kpn2I TCCGGA 1 cut(s) 1819
Kzo9I GATC 4 cut(s) 40, 154, 1357, 1886
LmnI GCTCC 5 cut(s) 319, 1217, 1726, 1734, 1812
Lsp1109I GCAGC 9 cut(s) 275, 314, 397, 400, 419, 428, 1232, 1381, 2045
LweI GCATC 5 cut(s) 116, 237, 880, 1408, 1430
MaeI CTAG 5 cut(s) 852, 1046, 1121, 1361, 1404
MaeII ACGT 3 cut(s) 967, 1150, 1384
MaeIII GTNAC 3 cut(s) 116, 506, 1194
MalI GATC 4 cut(s) 42, 156, 1359, 1888
MbiI CCGCTC 1 cut(s) 1729
MboI GATC 4 cut(s) 40, 154, 1357, 1886
MfeI CAATTG 1 cut(s) 435
MflI RGATCY 2 cut(s) 40, 1886
MhlI GDGCHC 1 cut(s) 1162
MlsI TGGCCA 1 cut(s) 885
MluNI TGGCCA 1 cut(s) 885
Mly113I GGCGCC 1 cut(s) 1847
MlyI GAGTC 3 cut(s) 590, 836, 1910
Mox20I TGGCCA 1 cut(s) 885
Mph1103I ATGCAT 2 cut(s) 902, 1423
MroI TCCGGA 1 cut(s) 1819
MroXI GAANNNNTTC 2 cut(s) 1089, 1311
MscI TGGCCA 1 cut(s) 885
MseI TTAA 3 cut(s) 741, 804, 1374
MslI CAYNNNNRTG 1 cut(s) 1539
Msp20I TGGCCA 1 cut(s) 885
MspA1I CMGCKG 2 cut(s) 410, 494
MspR9I CCNGG 4 cut(s) 338, 1898, 1984, 2109
MunI CAATTG 1 cut(s) 435
MvaI CCWGG 2 cut(s) 338, 1898
MvnI CGCG 1 cut(s) 1708
NarI GGCGCC 1 cut(s) 1847
NciI CCSGG 2 cut(s) 1984, 2109
NcoI CCATGG 2 cut(s) 886, 993
NdeII GATC 4 cut(s) 40, 154, 1357, 1886
NmuCI GTSAC 1 cut(s) 506
NruI TCGCGA 1 cut(s) 1708
NsiI ATGCAT 2 cut(s) 902, 1423
NspI RCATGY 3 cut(s) 50, 749, 904
PaeI GCATGC 1 cut(s) 904
PaqCI CACCTGC 2 cut(s) 833, 2038
PceI AGGCCT 1 cut(s) 1139
PciI ACATGT 2 cut(s) 46, 745
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PdmI GAANNNNTTC 2 cut(s) 1089, 1311
PfeI GAWTC 7 cut(s) 8, 131, 168, 277, 998, 1616, 2112
Pfl23II CGTACG 1 cut(s) 182
PflMI CCANNNNNTGG 1 cut(s) 1789
PfoI TCCNGGA 1 cut(s) 1896
PinAI ACCGGT 1 cut(s) 971
PleI GAGTC 3 cut(s) 589, 835, 1909
PluTI GGCGCC 1 cut(s) 1850
PpsI GAGTC 3 cut(s) 589, 835, 1909
PscI ACATGT 2 cut(s) 46, 745
PsiI TTATAA 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 1162
Psp6I CCWGG 2 cut(s) 336, 1896
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 336, 1896
PspLI CGTACG 1 cut(s) 182
PspPI GGNCC 4 cut(s) 929, 1252, 1916, 2104
PsrI GAACNNNNNNTAC 2 cut(s) 1074, 1106
PstI CTGCAG 3 cut(s) 418, 1627, 2035
PstNI CAGNNNCTG 2 cut(s) 104, 413
PsuI RGATCY 2 cut(s) 40, 1886
PvuII CAGCTG 1 cut(s) 410
RruI TCGCGA 1 cut(s) 1708
RsaI GTAC 6 cut(s) 184, 560, 970, 1260, 1333, 1678
RsaNI GTAC 6 cut(s) 183, 559, 969, 1259, 1332, 1677
RseI CAYNNNNRTG 1 cut(s) 1539
SacI GAGCTC 1 cut(s) 1162
SaqAI TTAA 3 cut(s) 741, 804, 1374
Sau3AI GATC 4 cut(s) 40, 154, 1357, 1886
Sau96I GGNCC 4 cut(s) 929, 1252, 1916, 2104
ScaI AGTACT 1 cut(s) 560
SchI GAGTC 3 cut(s) 590, 836, 1910
ScrFI CCNGG 4 cut(s) 338, 1898, 1984, 2109
SduI GDGCHC 1 cut(s) 1162
SfaNI GCATC 5 cut(s) 116, 237, 880, 1408, 1430
SfcI CTRYAG 4 cut(s) 414, 1184, 1623, 2031
SfoI GGCGCC 1 cut(s) 1848
SinI GGWCC 1 cut(s) 929
SmiMI CAYNNNNRTG 1 cut(s) 1539
SmlI CTYRAG 2 cut(s) 429, 1301
SmoI CTYRAG 2 cut(s) 429, 1301
SphI GCATGC 1 cut(s) 904
SseBI AGGCCT 1 cut(s) 1139
SsiI CCGC 8 cut(s) 492, 1325, 1605, 1729, 1732, 1870, 1873, 1914
SspDI GGCGCC 1 cut(s) 1846
SspMI CTAG 5 cut(s) 852, 1046, 1121, 1361, 1404
SstI GAGCTC 1 cut(s) 1162
StuI AGGCCT 1 cut(s) 1139
StyD4I CCNGG 4 cut(s) 336, 1896, 1982, 2107
StyI CCWWGG 3 cut(s) 681, 886, 993
TaaI ACNGT 4 cut(s) 218, 506, 660, 1194
TaiI ACGT 3 cut(s) 970, 1153, 1387
TaqI TCGA 5 cut(s) 129, 280, 357, 1156, 2023
TatI WGTACW 2 cut(s) 558, 1676
TauI GCSGC 3 cut(s) 1732, 1875, 1917
TfiI GAWTC 7 cut(s) 8, 131, 168, 277, 998, 1616, 2112
Tru1I TTAA 3 cut(s) 741, 804, 1374
Tru9I TTAA 3 cut(s) 741, 804, 1374
TscAI CASTG 5 cut(s) 129, 511, 628, 640, 1632
TseFI GTSAC 1 cut(s) 506
TseI GCWGC 9 cut(s) 288, 302, 407, 410, 413, 416, 1220, 1369, 2033
Tsp45I GTSAC 1 cut(s) 506
TspDTI ATGAA 9 cut(s) 21, 537, 579, 588, 669, 1044, 1296, 1764, 2059
TspRI CASTG 5 cut(s) 129, 511, 628, 640, 1632
Van91I CCANNNNNTGG 1 cut(s) 1789
VpaK11BI GGWCC 1 cut(s) 929
XagI CCTNNNNNAGG 1 cut(s) 693
XapI RAATTY 4 cut(s) 234, 1752, 2065, 2089
XbaI TCTAGA 2 cut(s) 851, 1120
XceI RCATGY 3 cut(s) 50, 749, 904
XmiI GTMKAC 1 cut(s) 830
XmnI GAANNNNTTC 2 cut(s) 1089, 1311
XspI CTAG 5 cut(s) 852, 1046, 1121, 1361, 1404
ZrmI AGTACT 1 cut(s) 560
Zsp2I ATGCAT 2 cut(s) 902, 1423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.