FvH4_5g35630

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007329
Physical Location & Seq
Reverse (-)
26079220 .. 26091194
11975 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35630.t3

Sequence Viewer

Length: 2655 bp
ATGACTAACATAATCCCGGATGCGCCTCAGGGGGATGATCAAGCTCCTCCTCCCCCTCCCCCAGCAGGCGGCCCTGATCATGCTGCTGATACTCAAGCTTCCCGCGGTGGCAATACGATTTTCAAGAGCGGGCCACTATTTATATCATCCAAAGGAATTGGATGGACATCCTGGAAGAAAAGATGGTTTATTTTAACGAAGACTTCGCTGGTTTTCTTCAGAAGTGATCCTAGTGCTGTTCAGAAGGGGAGTGAAGTGAATTTGACCCTAGGTGGTATTGACCTCAACAATTCAGGCAGTGTGGTTGTCAAAGAAGACAAGAAACTGTTGACTGTGCTCTTTCCAGATGGTCGTGATGGGCGAGCCTTCACACTGAAGGCTGAAACGTCGAAGGATCTACATGAATGGAAGGAAGCACTGGAGAACGCTTTGTCGCAAGCTCCAAGTGGCCCTCATGCTGTGGGAAAAAATGGTATCTTGGGGAATGATCAGAAAGACTCCAAAGATAGTACTTTGGATCAGCCAAAGGAGAAACCACCTGCGAAATCTACAATTATTGGGGTGCCTATTTTGCTGGCCTTGGAAGATGTTGATGGTGCTCCATGTTTCTTGGAAAAAGCCCTTAGGTTTGTAGAAGAATATGGAGTCAAGGTAGAAGGAATCTTGCGACAAGCTGCTGATGTTGATGATGTTGAACGTCGAATACGGGAATACGAGCAGGGGAAAGTTGAGTTTTCTCCTGATGAAGATCCACATATCATTGCTGATTGTGTCAAGTATGTTCTTCGGGAGTTGCCATCTTCTCCGGTCCCTGCATCTTGCTGCAACGCGCTGCTAGATTCCTTTCGCAAGTCCGGTAATGATCGGGGTGGAAGAATAAATGCCATGCGTACGACAATATGTGATACATTCCCGGAGCCAAACCGCTGTTTATTGCAAAGAATTCTATTAATGATGCAAACTATTGCTTCTCACAAAGCTGTGAATCGAATGAGCTGCTCAGCTGTGGCAGCTTGCATGGCCCCCTTACTTCTTCGTCCACTGCTGGCTGGGGATTGTGAAATCGACAATGATTTTAGCGTGGGTGGTGATAACTCTGTTCAACTTTTGCAAGCAGCTGCTGCAGCTAATCATGCTCAAGCAATTGTTATAACTTTGCTGGAGGAGTATGATAACATATTTGGGGAAGGTTCCCTGTCCCCTGAACTATACAGTGACACTGAAGATGATGAAACTGACACCGACGGGGCAACAGAAGATGATGAGTATTATGAAGATGATGAAACAGAATCTGGAGAATACGATGATGATGATGATGTTGAAATTGTCAGTGGGTCATGCAGTGAGAGTGATGGAGATGATGAACAATCTGACAATAAGGACGGACTTGGCTCAGGTTCCAAGGTTCCTGACGCAGGCGATGATAGCAAAGCTCATAAGAAATTGTCATCAAGATCACTTAAACATGCGAAGCCTCAAGTTGGTGTAGAAAGCAATGATGATAAACCGGATTTAAAGGAAAATAACTCAGCGGAGCTGGACAATGAGTTTACAGCAGTAGCAGATGTTTCTAGATCAACAAGTTTTGTGCACCAGTCAAGTGGCCATGGCCTTCCATGCATACAAAAATCTTCAAGTATATCCAATGGACCTGATTATGGTGCAAAGCATCGTTATAACTGGGGACGTACTGCTGCAAGGAAGAACCTTTCCATGGAATCCATTGGCTTCGGTGTTGAGGAGGAGGATGAACAGATCCAGAGGTTAGAGATCACAAAAGCTGACTTAGAAGATAGTATTGTAGAAGAGGTTCAAGATAATGTAGCTCTTCGAGCAAATCTAGAAATTGAGAAGAAGGCCTTGCGTGAGCGTCGTCGAGCTCTCGAACAAGATGTAGCAAGATTACAGGAACAGTTACAGAAGGAGAGAGATTTGGGAGCAGCCCTCGAGGCAGGTCTAAAAATATCCGAGGGCCATGTACCTAATTTAGCCAACGTTGATGAAATAACAAGGGCAGAGCTAGAGGAAATGGTTCAGGTAGAGGCAGATTGTACCAACTTGAAAAAGAAGGTTGATGATCTGGATGTGCAGCTTAATTTACAACGTGAACGAAACCATAGCTCTAGCTCTGCATCTGATGCATGCATTTTTTCTGCACATAGTCGGGATCATGATACAAAACAGCAGACGAAGAATAAAGAGAAGGATAGTGAAGCCGTAGCCCAGTTAGAACGGTCAAGAAGTAAGGATGGTCATCAAGAGGGCACAGAAAATGCAAATGAGAAAAAGGTGGAGCCAGTTGTTGCCAAGTCTAGCAAGAGAGGGAGTGAGGGGAGTAGAGGAATTACAAAGTTGACATCGCGACTAAACTTTTTGAAGGAGCGGCGGACTACTGAAGGTAGAGATAAAGTTCAATCGGTCCCAGATACTCTGGAAAAAGGCCAAAAGTCGGAAAAACTTAGGAAGGCAGAGAGTCATTCTGATCATAGAGGGAAAAAGTCAGAAGCCCAACAACAGCATATTCTGGACAGAGGAAAATCAGAAAGCCATCTGTCTATTGAAGTGGACAAAGGTCATGTCTTCGACAATAGTAGTAACAGTAGTTTTAGCACTAGTAAACCTTTCCCCCCTTCTCCAAGGAAAGACAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

885

Amino Acids

96.93

Weight (kDa)

4.99

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 42 - 144 3.6e-12 PH domain
RhoGAP PF00620 201 - 347 1.3e-27 RhoGAP domain
Lzipper-MIP1 PF14389 621 - 699 2.4e-15 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1151, 1677
AarI CACCTGC 1 cut(s) 547
AbsI CCTCGAGG 1 cut(s) 1946
Acc36I ACCTGC 2 cut(s) 547, 1943
AccB1I GGYRCC 1 cut(s) 562
AccBSI CCGCTC 2 cut(s) 129, 2383
AccII CGCG 3 cut(s) 105, 830, 2362
AciI CCGC 8 cut(s) 69, 103, 105, 129, 925, 1532, 2383, 2386
AclI AACGTT 1 cut(s) 1995
AclWI GGATC 6 cut(s) 221, 402, 525, 743, 1750, 2175
AcoI YGGCCR 1 cut(s) 1603
AcsI RAATTY 2 cut(s) 259, 942
AcuI CTGAAG 4 cut(s) 202, 395, 1242, 2415
AfaI GTAC 5 cut(s) 511, 892, 1690, 1980, 2053
AfiI CCNNNNNNNGG 7 cut(s) 65, 68, 1415, 1481, 1658, 1714, 2449
AhlI ACTAGT 1 cut(s) 2612
AjnI CCWGG 1 cut(s) 170
Alw21I GWGCWC 4 cut(s) 339, 601, 1593, 1882
Alw44I GTGCAC 1 cut(s) 1589
AlwI GGATC 6 cut(s) 221, 402, 525, 743, 1750, 2175
AlwNI CAGNNNCTG 2 cut(s) 1121, 1292
Ama87I CYCGRG 1 cut(s) 1946
ApaLI GTGCAC 1 cut(s) 1589
ApoI RAATTY 2 cut(s) 259, 942
ArsI GACNNNNNNTTYG 2 cut(s) 2562, 2594
AseI ATTAAT 1 cut(s) 950
Asp700I GAANNNNTTC 2 cut(s) 1809, 2031
AspA2I CCTAGG 1 cut(s) 268
AspLEI GCGC 2 cut(s) 25, 832
AspS9I GGNCC 8 cut(s) 71, 131, 449, 808, 1021, 1649, 1972, 2419
AsuC2I CCSGG 2 cut(s) 17, 914
AsuHPI GGTGA 1 cut(s) 1100
AvaI CYCGRG 1 cut(s) 1946
AvaII GGWCC 3 cut(s) 808, 1649, 2419
AvrII CCTAGG 1 cut(s) 268
AxyI CCTNAGG 2 cut(s) 27, 623
BaeGI GKGCMC 2 cut(s) 1593, 2267
BalI TGGCCA 1 cut(s) 1605
BanI GGYRCC 1 cut(s) 562
BanII GRGCYC 1 cut(s) 1882
BbsI GAAGAC 3 cut(s) 206, 321, 2572
Bbv12I GWGCWC 4 cut(s) 339, 601, 1593, 1882
BccI CCATC 9 cut(s) 156, 177, 341, 350, 587, 805, 1346, 2243, 2556
BceAI ACGGC 1 cut(s) 2201
BcgI CGANNNNNNTGC 2 cut(s) 978, 1012
BciT130I CCWGG 1 cut(s) 172
BclI TGATCA 4 cut(s) 37, 76, 487, 2482
BcnI CCSGG 2 cut(s) 17, 914
BcuI ACTAGT 1 cut(s) 2612
BfaI CTAG 9 cut(s) 231, 269, 836, 1572, 1841, 2021, 2124, 2313, 2613
BfmI CTRYAG 1 cut(s) 1122
BfuAI ACCTGC 2 cut(s) 547, 1943
BglI GCCNNNNNGGC 1 cut(s) 1949
BlnI CCTAGG 1 cut(s) 268
BlpI GCTNAGC 1 cut(s) 1000
BmcAI AGTACT 1 cut(s) 511
Bme1390I CCNGG 3 cut(s) 17, 172, 914
Bme18I GGWCC 3 cut(s) 808, 1649, 2419
BmeT110I CYCGRG 1 cut(s) 1946
BmgT120I GGNCC 8 cut(s) 71, 131, 449, 808, 1021, 1649, 1972, 2419
BmiI GGNNCC 9 cut(s) 564, 810, 918, 1023, 1192, 1399, 1407, 2295, 2421
BmrFI CCNGG 3 cut(s) 17, 172, 914
BmrI ACTGGG 2 cut(s) 1690, 2218
BmsI GCATC 6 cut(s) 10, 824, 945, 1678, 2128, 2141
BmuI ACTGGG 2 cut(s) 1690, 2218
BoxI GACNNNNGTC 1 cut(s) 2571
BpiI GAAGAC 3 cut(s) 206, 321, 2572
BplI GAGNNNNNCTC 2 cut(s) 1929, 1961
BpmI CTGGAG 3 cut(s) 440, 1181, 1314
Bpu10I CCTNAGC 1 cut(s) 1393
Bpu1102I GCTNAGC 1 cut(s) 1000
BpuEI CTTGAG 3 cut(s) 78, 1122, 1461
BpuMI CCSGG 2 cut(s) 17, 914
BsaBI GATNNNNATC 3 cut(s) 166, 747, 2253
BsaJI CCNNGG 8 cut(s) 103, 268, 579, 1401, 1606, 1713, 1968, 2636
BsaWI WCCGGW 3 cut(s) 805, 854, 1507
BsaXI ACNNNNNCTCC 2 cut(s) 636, 666
Bsc4I CCNNNNNNNGG 7 cut(s) 65, 68, 1415, 1481, 1658, 1714, 2449
Bse1I ACTGG 5 cut(s) 423, 1594, 1685, 2224, 2297
Bse21I CCTNAGG 2 cut(s) 27, 623
Bse3DI GCAATG 2 cut(s) 759, 1501
Bse8I GATNNNNATC 3 cut(s) 166, 747, 2253
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 8 cut(s) 103, 268, 579, 1401, 1606, 1713, 1968, 2636
BseGI GGATG 8 cut(s) 25, 40, 146, 167, 167, 1753, 2089, 2254
BseJI GATNNNNATC 3 cut(s) 166, 747, 2253
BseLI CCNNNNNNNGG 7 cut(s) 65, 68, 1415, 1481, 1658, 1714, 2449
BseMI GCAATG 2 cut(s) 759, 1501
BseMII CTCAG 4 cut(s) 41, 1014, 1407, 1542
BseNI ACTGG 5 cut(s) 423, 1594, 1685, 2224, 2297
BseRI GAGGAG 5 cut(s) 36, 39, 1178, 1754, 1757
BseSI GKGCMC 2 cut(s) 1593, 2267
BseYI CCCAGC 2 cut(s) 61, 1049
BsgI GTGCAG 2 cut(s) 2108, 2139
Bsh1236I CGCG 3 cut(s) 105, 830, 2362
BshNI GGYRCC 1 cut(s) 562
BsiHKAI GWGCWC 4 cut(s) 339, 601, 1593, 1882
BsiHKCI CYCGRG 1 cut(s) 1946
BsiSI CCGG 5 cut(s) 17, 806, 855, 914, 1508
BsiWI CGTACG 1 cut(s) 890
BslFI GGGAC 4 cut(s) 794, 1183, 1698, 2405
BslI CCNNNNNNNGG 7 cut(s) 65, 68, 1415, 1481, 1658, 1714, 2449
BsmFI GGGAC 4 cut(s) 794, 1183, 1698, 2405
BsoBI CYCGRG 1 cut(s) 1946
Bsp1286I GDGCHC 5 cut(s) 339, 601, 1593, 1882, 2267
Bsp1720I GCTNAGC 1 cut(s) 1000
Bsp19I CCATGG 2 cut(s) 1606, 1713
Bsp68I TCGCGA 1 cut(s) 2362
BspACI CCGC 8 cut(s) 69, 103, 105, 129, 925, 1532, 2383, 2386
BspCNI CTCAG 4 cut(s) 40, 1013, 1406, 1541
BspFNI CGCG 3 cut(s) 105, 830, 2362
BspHI TCATGA 1 cut(s) 2170
BspLI GGNNCC 9 cut(s) 564, 810, 918, 1023, 1192, 1399, 1407, 2295, 2421
BspMAI CTGCAG 1 cut(s) 1126
BspMI ACCTGC 2 cut(s) 547, 1943
BspPI GGATC 6 cut(s) 221, 402, 525, 743, 1750, 2175
BspQI GCTCTTC 1 cut(s) 1833
BspT107I GGYRCC 1 cut(s) 562
BsrBI CCGCTC 2 cut(s) 129, 2383
BsrDI GCAATG 2 cut(s) 759, 1501
BsrI ACTGG 5 cut(s) 423, 1594, 1685, 2224, 2297
BssECI CCNNGG 8 cut(s) 103, 268, 579, 1401, 1606, 1713, 1968, 2636
BssT1I CCWWGG 6 cut(s) 268, 579, 1401, 1606, 1713, 2636
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 6 cut(s) 327, 334, 1214, 1914, 2235, 2600
Bst6I CTCTTC 2 cut(s) 1800, 1833
BstAPI GCANNNNNTGC 2 cut(s) 1121, 2138
BstDEI CTNAG 7 cut(s) 27, 623, 1000, 1393, 1528, 1786, 2459
BstDSI CCRYGG 3 cut(s) 103, 1606, 1713
BstENI CCTNNNNNAGG 1 cut(s) 1413
BstF5I GGATG 8 cut(s) 25, 40, 146, 167, 167, 1753, 2089, 2254
BstFNI CGCG 3 cut(s) 105, 830, 2362
BstHHI GCGC 2 cut(s) 25, 832
BstNI CCWGG 1 cut(s) 172
BstNSI RCATGY 2 cut(s) 1469, 2145
BstPAI GACNNNNGTC 1 cut(s) 2571
BstSCI CCNGG 3 cut(s) 15, 170, 912
BstSFI CTRYAG 1 cut(s) 1122
BstSLI GKGCMC 2 cut(s) 1593, 2267
BstUI CGCG 3 cut(s) 105, 830, 2362
BstV2I GAAGAC 3 cut(s) 206, 321, 2572
BstX2I RGATCY 3 cut(s) 394, 748, 1755
BstXI CCANNNNNNTGG 1 cut(s) 1601
BstYI RGATCY 3 cut(s) 394, 748, 1755
Bsu36I CCTNAGG 2 cut(s) 27, 623
BtgI CCRYGG 3 cut(s) 103, 1606, 1713
BtgZI GCGATG 2 cut(s) 1434, 2343
BtsCI GGATG 8 cut(s) 25, 40, 146, 167, 167, 1753, 2089, 2254
BtsI GCAGTG 3 cut(s) 304, 1040, 1348
BtsIMutI CAGTG 8 cut(s) 304, 371, 416, 1040, 1218, 1219, 1336, 1348
BtuMI TCGCGA 1 cut(s) 2362
BveI ACCTGC 2 cut(s) 547, 1943
CaiI CAGNNNCTG 2 cut(s) 1121, 1292
CciI TCATGA 1 cut(s) 2170
CfoI GCGC 2 cut(s) 25, 832
Cfr13I GGNCC 8 cut(s) 71, 131, 449, 808, 1021, 1649, 1972, 2419
Cfr42I CCGCGG 1 cut(s) 106
CseI GACGC 2 cut(s) 1421, 1859
Csp6I GTAC 5 cut(s) 510, 891, 1689, 1979, 2052
CviQI GTAC 5 cut(s) 510, 891, 1689, 1979, 2052
DdeI CTNAG 7 cut(s) 27, 623, 1000, 1393, 1528, 1786, 2459
DraI TTTAAA 1 cut(s) 1515
EaeI YGGCCR 1 cut(s) 1603
Eam1104I CTCTTC 2 cut(s) 1800, 1833
EarI CTCTTC 2 cut(s) 1800, 1833
EciI GGCGGA 1 cut(s) 2401
Ecl136II GAGCTC 1 cut(s) 1880
Eco130I CCWWGG 6 cut(s) 268, 579, 1401, 1606, 1713, 2636
Eco147I AGGCCT 1 cut(s) 1859
Eco24I GRGCYC 1 cut(s) 1882
Eco47I GGWCC 3 cut(s) 808, 1649, 2419
Eco53kI GAGCTC 1 cut(s) 1880
Eco57I CTGAAG 4 cut(s) 202, 395, 1242, 2415
Eco81I CCTNAGG 2 cut(s) 27, 623
Eco88I CYCGRG 1 cut(s) 1946
EcoICRI GAGCTC 1 cut(s) 1880
EcoNI CCTNNNNNAGG 1 cut(s) 1413
EcoRI GAATTC 1 cut(s) 942
EcoRII CCWGG 1 cut(s) 170
EcoT14I CCWWGG 6 cut(s) 268, 579, 1401, 1606, 1713, 2636
EcoT22I ATGCAT 3 cut(s) 1622, 2143, 2147
EcoT38I GRGCYC 1 cut(s) 1882
ErhI CCWWGG 6 cut(s) 268, 579, 1401, 1606, 1713, 2636
FalI AAGNNNNNCTT 2 cut(s) 1844, 1876
FaqI GGGAC 4 cut(s) 794, 1183, 1698, 2405
FauI CCCGC 2 cut(s) 110, 122
FbaI TGATCA 4 cut(s) 37, 76, 487, 2482
FokI GGATG 8 cut(s) 32, 47, 133, 154, 174, 1760, 2096, 2261
FriOI GRGCYC 1 cut(s) 1882
FspBI CTAG 9 cut(s) 231, 269, 836, 1572, 1841, 2021, 2124, 2313, 2613
GlaI GCGC 2 cut(s) 24, 831
GsaI CCCAGC 2 cut(s) 65, 1053
GsuI CTGGAG 3 cut(s) 440, 1181, 1314
HapII CCGG 5 cut(s) 17, 806, 855, 914, 1508
HgaI GACGC 2 cut(s) 1421, 1859
HhaI GCGC 2 cut(s) 25, 832
Hin6I GCGC 2 cut(s) 23, 830
HinP1I GCGC 2 cut(s) 23, 830
HincII GTYRAC 2 cut(s) 330, 2355
HindII GTYRAC 2 cut(s) 330, 2355
HindIII AAGCTT 1 cut(s) 96
HinfI GANTC 8 cut(s) 497, 645, 660, 839, 985, 1289, 1718, 2473
HpaII CCGG 5 cut(s) 17, 806, 855, 914, 1508
HphI GGTGA 1 cut(s) 1100
Hpy166II GTNNAC 8 cut(s) 330, 1040, 1551, 1591, 2108, 2355, 2566, 2618
Hpy8I GTNNAC 8 cut(s) 330, 1040, 1551, 1591, 2108, 2355, 2566, 2618
Hpy99I CGWCG 5 cut(s) 391, 702, 1247, 1875, 1878
HpyCH4III ACNGT 6 cut(s) 327, 334, 1214, 1914, 2235, 2600
HpyCH4IV ACGT 5 cut(s) 386, 697, 1687, 1995, 2104
HpyF3I CTNAG 7 cut(s) 27, 623, 1000, 1393, 1528, 1786, 2459
HpySE526I ACGT 5 cut(s) 386, 697, 1687, 1995, 2104
HspAI GCGC 2 cut(s) 23, 830
Ksp22I TGATCA 4 cut(s) 37, 76, 487, 2482
KspI CCGCGG 1 cut(s) 106
LguI GCTCTTC 1 cut(s) 1833
LmnI GCTCC 8 cut(s) 49, 445, 604, 916, 1534, 1937, 2293, 2380
LweI GCATC 6 cut(s) 10, 824, 945, 1678, 2128, 2141
MaeI CTAG 9 cut(s) 231, 269, 836, 1572, 1841, 2021, 2124, 2313, 2613
MaeII ACGT 5 cut(s) 386, 697, 1687, 1995, 2104
MaeIII GTNAC 3 cut(s) 1214, 1914, 2594
MbiI CCGCTC 2 cut(s) 129, 2383
MfeI CAATTG 1 cut(s) 1143
MflI RGATCY 3 cut(s) 394, 748, 1755
MhlI GDGCHC 5 cut(s) 339, 601, 1593, 1882, 2267
MlsI TGGCCA 1 cut(s) 1605
MluNI TGGCCA 1 cut(s) 1605
MlyI GAGTC 3 cut(s) 491, 654, 2482
MmeI TCCRAC 1 cut(s) 2430
Mox20I TGGCCA 1 cut(s) 1605
Mph1103I ATGCAT 3 cut(s) 1622, 2143, 2147
MroXI GAANNNNTTC 2 cut(s) 1809, 2031
MscI TGGCCA 1 cut(s) 1605
MseI TTAA 5 cut(s) 194, 950, 1461, 1514, 2094
Msp20I TGGCCA 1 cut(s) 1605
MspA1I CMGCKG 5 cut(s) 105, 927, 1004, 1118, 1532
MspI CCGG 5 cut(s) 17, 806, 855, 914, 1508
MspR9I CCNGG 3 cut(s) 17, 172, 914
MunI CAATTG 1 cut(s) 1143
MvaI CCWGG 1 cut(s) 172
MvnI CGCG 3 cut(s) 105, 830, 2362
NciI CCSGG 2 cut(s) 17, 914
NcoI CCATGG 2 cut(s) 1606, 1713
NlaIV GGNNCC 9 cut(s) 564, 810, 918, 1023, 1192, 1399, 1407, 2295, 2421
NmuCI GTSAC 1 cut(s) 1214
NruI TCGCGA 1 cut(s) 2362
NsiI ATGCAT 3 cut(s) 1622, 2143, 2147
NspI RCATGY 2 cut(s) 1469, 2145
PaeI GCATGC 1 cut(s) 2145
PaeR7I CTCGAG 1 cut(s) 1946
PagI TCATGA 1 cut(s) 2170
PaqCI CACCTGC 1 cut(s) 547
PceI AGGCCT 1 cut(s) 1859
PciSI GCTCTTC 1 cut(s) 1833
PcsI WCGNNNNNNNCGW 1 cut(s) 358
PdmI GAANNNNTTC 2 cut(s) 1809, 2031
PfeI GAWTC 5 cut(s) 660, 839, 985, 1289, 1718
Pfl23II CGTACG 1 cut(s) 890
PfoI TCCNGGA 3 cut(s) 15, 170, 912
PleI GAGTC 3 cut(s) 491, 653, 2481
PpsI GAGTC 3 cut(s) 491, 653, 2481
PshAI GACNNNNGTC 1 cut(s) 2571
PshBI ATTAAT 1 cut(s) 950
PsiI TTATAA 2 cut(s) 1151, 1677
Psp124BI GAGCTC 1 cut(s) 1882
Psp1406I AACGTT 1 cut(s) 1995
Psp6I CCWGG 1 cut(s) 170
PspFI CCCAGC 2 cut(s) 61, 1049
PspGI CCWGG 1 cut(s) 170
PspLI CGTACG 1 cut(s) 890
PspN4I GGNNCC 9 cut(s) 564, 810, 918, 1023, 1192, 1399, 1407, 2295, 2421
PspPI GGNCC 8 cut(s) 71, 131, 449, 808, 1021, 1649, 1972, 2419
PspXI VCTCGAGB 1 cut(s) 1946
PsrI GAACNNNNNNTAC 4 cut(s) 687, 719, 1794, 1826
PstI CTGCAG 1 cut(s) 1126
PstNI CAGNNNCTG 2 cut(s) 1121, 1292
PsuI RGATCY 3 cut(s) 394, 748, 1755
PvuII CAGCTG 2 cut(s) 1004, 1118
RruI TCGCGA 1 cut(s) 2362
RsaI GTAC 5 cut(s) 511, 892, 1690, 1980, 2053
RsaNI GTAC 5 cut(s) 510, 891, 1689, 1979, 2052
SacI GAGCTC 1 cut(s) 1882
SacII CCGCGG 1 cut(s) 106
SapI GCTCTTC 1 cut(s) 1833
SaqAI TTAA 5 cut(s) 194, 950, 1461, 1514, 2094
Sau96I GGNCC 8 cut(s) 71, 131, 449, 808, 1021, 1649, 1972, 2419
ScaI AGTACT 1 cut(s) 511
SchI GAGTC 3 cut(s) 491, 654, 2482
ScrFI CCNGG 3 cut(s) 17, 172, 914
SduI GDGCHC 5 cut(s) 339, 601, 1593, 1882, 2267
SfaNI GCATC 6 cut(s) 10, 824, 945, 1678, 2128, 2141
SfcI CTRYAG 1 cut(s) 1122
Sfr274I CTCGAG 1 cut(s) 1946
Sfr303I CCGCGG 1 cut(s) 106
SgrBI CCGCGG 1 cut(s) 106
SinI GGWCC 3 cut(s) 808, 1649, 2419
SlaI CTCGAG 1 cut(s) 1946
SmlI CTYRAG 4 cut(s) 93, 1137, 1476, 1946
SmoI CTYRAG 4 cut(s) 93, 1137, 1476, 1946
SpeI ACTAGT 1 cut(s) 2612
SphI GCATGC 1 cut(s) 2145
SseBI AGGCCT 1 cut(s) 1859
SsiI CCGC 8 cut(s) 69, 103, 105, 129, 925, 1532, 2383, 2386
SspMI CTAG 9 cut(s) 231, 269, 836, 1572, 1841, 2021, 2124, 2313, 2613
SstI GAGCTC 1 cut(s) 1882
StuI AGGCCT 1 cut(s) 1859
StyD4I CCNGG 3 cut(s) 15, 170, 912
StyI CCWWGG 6 cut(s) 268, 579, 1401, 1606, 1713, 2636
TaaI ACNGT 6 cut(s) 327, 334, 1214, 1914, 2235, 2600
TaiI ACGT 5 cut(s) 389, 700, 1690, 1998, 2107
TaqI TCGA 9 cut(s) 389, 700, 988, 1065, 1831, 1876, 1884, 1947, 2583
TaqII GACCGA 1 cut(s) 2407
TatI WGTACW 1 cut(s) 509
TauI GCSGC 2 cut(s) 72, 2386
TfiI GAWTC 5 cut(s) 660, 839, 985, 1289, 1718
Tru1I TTAA 5 cut(s) 194, 950, 1461, 1514, 2094
Tru9I TTAA 5 cut(s) 194, 950, 1461, 1514, 2094
TscAI CASTG 8 cut(s) 304, 378, 423, 1047, 1219, 1225, 1336, 1348
TseFI GTSAC 1 cut(s) 1214
Tsp45I GTSAC 1 cut(s) 1214
TspDTI ATGAA 8 cut(s) 417, 759, 1245, 1287, 1296, 1377, 1764, 2016
TspGWI ACGGA 1 cut(s) 1398
TspRI CASTG 8 cut(s) 304, 378, 423, 1047, 1219, 1225, 1336, 1348
VneI GTGCAC 1 cut(s) 1589
VpaK11BI GGWCC 3 cut(s) 808, 1649, 2419
VspI ATTAAT 1 cut(s) 950
XagI CCTNNNNNAGG 1 cut(s) 1413
XapI RAATTY 2 cut(s) 259, 942
XbaI TCTAGA 2 cut(s) 1571, 1840
XceI RCATGY 2 cut(s) 1469, 2145
XhoI CTCGAG 1 cut(s) 1946
XmaJI CCTAGG 1 cut(s) 268
XmnI GAANNNNTTC 2 cut(s) 1809, 2031
XspI CTAG 9 cut(s) 231, 269, 836, 1572, 1841, 2021, 2124, 2313, 2613
ZrmI AGTACT 1 cut(s) 511
Zsp2I ATGCAT 3 cut(s) 1622, 2143, 2147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.