RLG00000001032

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0007329
Physical Location & Seq
Forward (+)
9461907 .. 9473485
11579 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001032

Sequence Viewer

Length: 2856 bp
ATGGTTGTGCAGGGTGTTGATCTATATTGGCTGAGGTGTGGGGGATGTTTCTCTAACGTTTGGGATCAGGAAATGACTAACATAATCCCGGAAGCGCCTCAGGGGGATAGACATGCTTCTCCTCCCCAACCCCCGCCTGGCGGCCCTGGTGGCGACCCCGATCATCATGCTGAATCTCATGGTTCTCGTGGTGGCAATACGGTTTTCAAGAGTGGGCCACTATTTATATCTTCCAAAGGAATTGGATGGACATCCTGGAAAAAGAGATGGTTTATTTTAACTCGTACTTCGCTGGTTTTCTTCAGAAGTGATCCTAGTGCTGTTCAGAAGGGGAGTGAAGTGAATTTGACCCTTGGTGGTATTGACCTCAACAATTCAGGCAGTGTGGTTGTCAAAGAAGACAAGAAACTCTTGACTGTACTCTTTCCAGATGGTCGTGATGGGCGAGCCTTCACACTTAAGGCTGAAACCTTGAAAGATCTACATGAATGGAAGGAAGCACTGGATAACGCTTTGGCACAAGCTCCAAGTGGCGCTCATGGTGTGGGGCAAAATGGTATCTTGGGGAATGATCAGACAGACTCCAAAAATGGTTCTTTGGACCAGCTAAAGGAGAAACCACCTGTGAAATCTACAATTATTGGGGTGCCTATTTTGCTGGCCTTGGAAGATGTTGATGGAGCTCCATGTTTCTTGGAAAAAGCCCTTAGGTTCCTAGAAGAACATGGAGTCAAGGTAGAAGGAATCTTGCGACAAGCTGCAGATGTTGATGACGTTGAATCTCGAATACGGGAATATGAGAAGGGGAAAGTTGATTTTTCGCCCGAGGAGGATCCACATGTCATTGCTGATTGTGTCAAGTATGTCCTCCGAGAGTTGCCATCTTCTCCAGTCCCTGCATCTTGCTGTAACGCACTGCTAGATTCCTTTCGCAAGTCTGGTAATGATCGGGGTGGTAGAATCAATGCCATGCGTACGACAATATGTGATACATTCCCGGAGCCAAACCGTTGTTTATTGCAAAGAATTCTATTGATGATGCAAACTATTGCTTCTCACAAAGCCGTGAATCGAATGAGCTGCTCAGCCGTGGCAGCTTGCATGGCTCCCTTACTTCTTCGTCCACTCCTAGCTGGGGATTGTGAAATCGACAATGATTTTAGTGTGGGTGGCGACAATTCGGTTCAGCTTTTGCAAGCAGCTGCTGCAGCTAATCATGCTCAAGCAATTGTTATAACTTTGCTGGAGGAGTATGATAACATATTTGGGGAAGGTTCCATATCCGCTGAACTATACAGTGACACAGACGATGATGAAACTGAAACCGAGGGGGAAACTGAAGATGATGAGTACTATGAAGATGATGAAACTGAGTCTGGAGAATACGATGATGATGATGATGTTGAAATTGTCAGTGGGTCATGCAGTGAGAGTGATGAAGATGATGAACAGTTTGACAATAAGGCTGGTTCCAAGGTTCCGGATGGAGGCAATGATAGCAAATCTCCAAAGTTGTCATCAAGCTCACTTAAACATGTGAAGCATCAATATAGTGTAAAAAGCAATGATAATAAACAGAATGCAAGTGAAATTAACTCAGCAGAGCTGGCTAATGAGTCGACTGGAGTAGCAGGTGTTTCTAGAGAAACAAGTTCTGTGCATCAGTCAACTGGCCATGGCCTTCCATGCATGCAAAAATCTTCAACTATATCCAATGGACCTGATTATGGTGCAACGCATCGTCCTAACTGGGGACGTACCGGTGCAAGGAAGAACCTTTCCATGGAATCCATTGACTTCGGTGTTGAGGAGGAGGATGAAGAAATCCAGAGGCTAGAGATTACAAAAGCTGAGTTAGAAAATAAAATTGTAGAAGAGGTTCAAGGGAATGTAGCTCTGCAAGCAAATCTAGAAAAGCAGAAGGCGGCCTTGCTTGAACGTCGTCAAGCTCTCAAACAAGATGTGGCAAGACTACAGGAACAGTTACAGAAGGAGAGAGATTTGGGAGCAGCCCTTGAAGCAGGGCTAAAAATCTCCGAGGGCCATGTACCTAATTTAGCCGATGTTGATGAAAAAACAAGGGCAGAGCTTGAGGAAATGGTTCAGGCAGAGGCAGATTGTACCAACATGAAAAAGAAGGTTGATGATCTAGGTGTGCAGCTTAATCAACAACGTGAACGAAACTATGGCTCTAGCTCTGCGTCTGATGCAACCAATTTGTCGCGACATAGTCATAATATAAAACAGCAGATGAAGGATAAACAGAAGGATAGTGAAGCAGTGGTCCCTTCACATTTTGAACGGTCAAGAAGTAAGGATGATCATCAAGATGGCACAGAAAATGCAAATGAGAGGAAGGTGGATGGACTACATAAACTCTCCCGACTTGTTGGCGGTATATCCGATTCTTCTGCAGTGGAACCAGTTGTTGCCAAGCATGGCAAGAAAGGGGGTGAGGGGGGTAGTACATCTTCAGCAATAACAAAGTTGACATCGCGACTAAACTTTTTGAAGGAGCGGCGGAGCCAAATAGCCAATGAAATTCAAAGCAGAGATAAAGTTCAGTGTTCCCAAAACTTGGAGAAATGTCAATTGCTCCCAGATAATCTGGAAAAAGTTAGAGGATTGGAAGGGGCAGTATCAAGCCAAAAGTCAGAAAAACTCAGAAAGGCTGAGAGTCACTCTGAGAGTAGAGGGAAAAAGTCAGAAGCCCAACAACAGCATATCCTGGACAGAGGAAAATCAGAAAGCCATCTTTCTGTTGACGTGGACAAAGGTCGAGTCATTGACAGTAGTAGGAGTAGTAGCAGCAGCAGTAGTAAACCTTTCCCTTCTCCAAGGAAAGACAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

952

Amino Acids

103.9

Weight (kDa)

5.13

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 69 - 171 2.8e-11 PH domain
RhoGAP PF00620 229 - 375 6.6e-28 RhoGAP domain
Lzipper-MIP1 PF14389 646 - 724 9.2e-16 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1235
AarI CACCTGC 1 cut(s) 1622
Acc36I ACCTGC 1 cut(s) 1622
AccB1I GGYRCC 1 cut(s) 646
AccB7I CCANNNNNTGG 1 cut(s) 2578
AccBSI CCGCTC 1 cut(s) 2518
AccI GTMKAC 1 cut(s) 1619
AccII CGCG 2 cut(s) 2224, 2497
AccIII TCCGGA 1 cut(s) 1480
AciI CCGC 7 cut(s) 134, 141, 1284, 1925, 2394, 2518, 2521
AclI AACGTT 1 cut(s) 57
AclWI GGATC 4 cut(s) 72, 305, 827, 840
AcoI YGGCCR 1 cut(s) 1672
AcsI RAATTY 3 cut(s) 343, 1026, 2541
AcuI CTGAAG 3 cut(s) 286, 1359, 2457
AfaI GTAC 8 cut(s) 286, 420, 976, 1352, 1759, 2049, 2122, 2467
AflII CTTAAG 1 cut(s) 458
AflIII ACRYGT 2 cut(s) 838, 1534
AgeI ACCGGT 1 cut(s) 1760
AjiI CACGTC 1 cut(s) 2767
AjnI CCWGG 4 cut(s) 136, 145, 254, 2727
Alw21I GWGCWC 1 cut(s) 685
AlwI GGATC 4 cut(s) 72, 305, 827, 840
AlwNI CAGNNNCTG 2 cut(s) 896, 1205
Ama87I CYCGRG 1 cut(s) 824
Aor13HI TCCGGA 1 cut(s) 1480
AoxI GGCC 7 cut(s) 142, 215, 660, 1672, 1678, 1926, 2041
ApoI RAATTY 3 cut(s) 343, 1026, 2541
ArsI GACNNNNNNTTYG 2 cut(s) 2766, 2798
AsiGI ACCGGT 1 cut(s) 1760
Asp700I GAANNNNTTC 2 cut(s) 1878, 2100
AspLEI GCGC 2 cut(s) 97, 536
AspS9I GGNCC 6 cut(s) 143, 215, 601, 1718, 2041, 2284
AsuC2I CCSGG 2 cut(s) 89, 998
AsuHPI GGTGA 1 cut(s) 2465
AvaI CYCGRG 1 cut(s) 824
AvaII GGWCC 3 cut(s) 601, 1718, 2284
AxyI CCTNAGG 2 cut(s) 99, 707
BalI TGGCCA 1 cut(s) 1674
BamHI GGATCC 1 cut(s) 832
BanI GGYRCC 1 cut(s) 646
BanII GRGCYC 1 cut(s) 685
BarI GAAGNNNNNNTAC 2 cut(s) 2455, 2487
BauI CACGAG 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 685
BbvCI CCTCAGC 1 cut(s) 32
BceAI ACGGC 2 cut(s) 1049, 1073
BcgI CGANNNNNNTGC 6 cut(s) 149, 183, 1062, 1096, 2393, 2427
BciT130I CCWGG 4 cut(s) 138, 147, 256, 2729
BclI TGATCA 2 cut(s) 571, 2320
BcnI CCSGG 2 cut(s) 89, 998
BfaI CTAG 9 cut(s) 315, 716, 920, 1130, 1641, 1835, 1910, 2150, 2193
BfmI CTRYAG 4 cut(s) 759, 1206, 1973, 2412
BfoI RGCGCY 2 cut(s) 98, 537
BfrI CTTAAG 1 cut(s) 458
BfuAI ACCTGC 1 cut(s) 1622
BglI GCCNNNNNGGC 1 cut(s) 150
BglII AGATCT 1 cut(s) 478
BlpI GCTNAGC 1 cut(s) 1084
BmcAI AGTACT 1 cut(s) 1352
Bme1390I CCNGG 6 cut(s) 89, 138, 147, 256, 998, 2729
Bme18I GGWCC 3 cut(s) 601, 1718, 2284
BmeT110I CYCGRG 1 cut(s) 824
BmgBI CACGTC 1 cut(s) 2767
BmgT120I GGNCC 6 cut(s) 143, 215, 601, 1718, 2041, 2284
BmrFI CCNGG 6 cut(s) 89, 138, 147, 256, 998, 2729
BmrI ACTGGG 1 cut(s) 1759
BmsI GCATC 6 cut(s) 908, 1029, 1552, 1669, 1747, 2197
BmuI ACTGGG 1 cut(s) 1759
BoxI GACNNNNGTC 1 cut(s) 2775
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 2666, 2698
BpmI CTGGAG 4 cut(s) 873, 1265, 1398, 1644
Bpu10I CCTNAGC 1 cut(s) 32
Bpu1102I GCTNAGC 1 cut(s) 1084
BpuEI CTTGAG 2 cut(s) 1206, 2111
BpuMI CCSGG 2 cut(s) 89, 998
BsaBI GATNNNNATC 2 cut(s) 250, 2322
BsaWI WCCGGW 2 cut(s) 1480, 1760
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bse118I RCCGGY 1 cut(s) 1760
Bse1I ACTGG 6 cut(s) 507, 890, 1627, 1675, 1754, 2423
Bse21I CCTNAGG 2 cut(s) 99, 707
Bse3DI GCAATG 3 cut(s) 843, 1498, 1570
Bse8I GATNNNNATC 2 cut(s) 250, 2322
BseAI TCCGGA 1 cut(s) 1480
BseBI CCWGG 4 cut(s) 138, 147, 256, 2729
BseGI GGATG 7 cut(s) 50, 251, 251, 1489, 1822, 2323, 2368
BseJI GATNNNNATC 2 cut(s) 250, 2322
BseMI GCAATG 3 cut(s) 843, 1498, 1570
BseMII CTCAG 9 cut(s) 23, 113, 1098, 1362, 1611, 1842, 2664, 2676, 2677
BseNI ACTGG 6 cut(s) 507, 890, 1627, 1675, 1754, 2423
BseRI GAGGAG 5 cut(s) 111, 842, 1262, 1823, 1826
BseYI CCCAGC 1 cut(s) 1133
BsgI GTGCAG 2 cut(s) 29, 2177
Bsh1236I CGCG 2 cut(s) 2224, 2497
BshFI GGCC 7 cut(s) 144, 217, 662, 1674, 1680, 1928, 2043
BshNI GGYRCC 1 cut(s) 646
BshTI ACCGGT 1 cut(s) 1760
BsiHKAI GWGCWC 1 cut(s) 685
BsiHKCI CYCGRG 1 cut(s) 824
BsiSI CCGG 4 cut(s) 89, 998, 1481, 1761
BsiWI CGTACG 1 cut(s) 974
BslFI GGGAC 3 cut(s) 878, 1767, 2270
BsmFI GGGAC 3 cut(s) 878, 1767, 2270
BsmI GAATGC 1 cut(s) 1585
BsnI GGCC 7 cut(s) 144, 217, 662, 1674, 1680, 1928, 2043
BsoBI CYCGRG 1 cut(s) 824
Bsp1286I GDGCHC 1 cut(s) 685
Bsp13I TCCGGA 1 cut(s) 1480
Bsp1720I GCTNAGC 1 cut(s) 1084
Bsp19I CCATGG 2 cut(s) 1675, 1782
Bsp68I TCGCGA 2 cut(s) 2224, 2497
BspACI CCGC 7 cut(s) 134, 141, 1284, 1925, 2394, 2518, 2521
BspANI GGCC 7 cut(s) 144, 217, 662, 1674, 1680, 1928, 2043
BspCNI CTCAG 9 cut(s) 24, 112, 1097, 1363, 1610, 1843, 2665, 2676, 2677
BspEI TCCGGA 1 cut(s) 1480
BspFNI CGCG 2 cut(s) 2224, 2497
BspMAI CTGCAG 3 cut(s) 763, 1210, 2416
BspMI ACCTGC 1 cut(s) 1622
BspPI GGATC 4 cut(s) 72, 305, 827, 840
BspT107I GGYRCC 1 cut(s) 646
BspTI CTTAAG 1 cut(s) 458
BsrBI CCGCTC 1 cut(s) 2518
BsrDI GCAATG 3 cut(s) 843, 1498, 1570
BsrFI RCCGGY 1 cut(s) 1760
BsrI ACTGG 6 cut(s) 507, 890, 1627, 1675, 1754, 2423
BssAI RCCGGY 1 cut(s) 1760
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 6 cut(s) 352, 663, 1473, 1675, 1782, 2837
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 4 cut(s) 138, 147, 256, 2729
Bst4CI ACNGT 8 cut(s) 202, 418, 1010, 1298, 1452, 1983, 2304, 2792
Bst6I CTCTTC 1 cut(s) 1869
BstAFI CTTAAG 1 cut(s) 458
BstAPI GCANNNNNTGC 1 cut(s) 1205
BstC8I GCNNGC 7 cut(s) 447, 660, 1099, 1197, 1608, 1691, 1902
BstDSI CCRYGG 3 cut(s) 1089, 1675, 1782
BstF5I GGATG 7 cut(s) 50, 251, 251, 1489, 1822, 2323, 2368
BstFNI CGCG 2 cut(s) 2224, 2497
BstH2I RGCGCY 2 cut(s) 98, 537
BstHHI GCGC 2 cut(s) 97, 536
BstNI CCWGG 4 cut(s) 138, 147, 256, 2729
BstNSI RCATGY 4 cut(s) 116, 842, 1538, 1693
BstPAI GACNNNNGTC 1 cut(s) 2775
BstSCI CCNGG 6 cut(s) 87, 136, 145, 254, 996, 2727
BstSFI CTRYAG 4 cut(s) 759, 1206, 1973, 2412
BstUI CGCG 2 cut(s) 2224, 2497
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 2 cut(s) 478, 832
BstYI RGATCY 2 cut(s) 478, 832
Bsu36I CCTNAGG 2 cut(s) 99, 707
BsuRI GGCC 7 cut(s) 144, 217, 662, 1674, 1680, 1928, 2043
BtgI CCRYGG 3 cut(s) 1089, 1675, 1782
BtgZI GCGATG 1 cut(s) 2478
BtrI CACGTC 1 cut(s) 2767
BtsCI GGATG 7 cut(s) 50, 251, 251, 1489, 1822, 2323, 2368
BtsI GCAGTG 5 cut(s) 388, 914, 1432, 2286, 2421
BtsIMutI CAGTG 9 cut(s) 388, 500, 914, 1303, 1420, 1432, 2286, 2421, 2570
BtuMI TCGCGA 2 cut(s) 2224, 2497
BveI ACCTGC 1 cut(s) 1622
Cac8I GCNNGC 7 cut(s) 447, 660, 1099, 1197, 1608, 1691, 1902
CaiI CAGNNNCTG 2 cut(s) 896, 1205
CfoI GCGC 2 cut(s) 97, 536
Cfr10I RCCGGY 1 cut(s) 1760
Cfr13I GGNCC 6 cut(s) 143, 215, 601, 1718, 2041, 2284
CseI GACGC 1 cut(s) 2190
Csp6I GTAC 8 cut(s) 285, 419, 975, 1351, 1758, 2048, 2121, 2466
CspAI ACCGGT 1 cut(s) 1760
CviQI GTAC 8 cut(s) 285, 419, 975, 1351, 1758, 2048, 2121, 2466
EaeI YGGCCR 1 cut(s) 1672
Eam1104I CTCTTC 1 cut(s) 1869
EarI CTCTTC 1 cut(s) 1869
EciI GGCGGA 1 cut(s) 2536
Ecl136II GAGCTC 1 cut(s) 683
Eco130I CCWWGG 6 cut(s) 352, 663, 1473, 1675, 1782, 2837
Eco24I GRGCYC 1 cut(s) 685
Eco47I GGWCC 3 cut(s) 601, 1718, 2284
Eco53kI GAGCTC 1 cut(s) 683
Eco57I CTGAAG 3 cut(s) 286, 1359, 2457
Eco81I CCTNAGG 2 cut(s) 99, 707
Eco88I CYCGRG 1 cut(s) 824
EcoICRI GAGCTC 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 1026
EcoRII CCWGG 4 cut(s) 136, 145, 254, 2727
EcoT14I CCWWGG 6 cut(s) 352, 663, 1473, 1675, 1782, 2837
EcoT22I ATGCAT 1 cut(s) 1691
EcoT38I GRGCYC 1 cut(s) 685
ErhI CCWWGG 6 cut(s) 352, 663, 1473, 1675, 1782, 2837
FalI AAGNNNNNCTT 4 cut(s) 395, 427, 1913, 1945
FaqI GGGAC 3 cut(s) 878, 1767, 2270
FauI CCCGC 1 cut(s) 141
FbaI TGATCA 2 cut(s) 571, 2320
FblI GTMKAC 1 cut(s) 1619
FokI GGATG 7 cut(s) 57, 238, 258, 1496, 1829, 2330, 2375
FriOI GRGCYC 1 cut(s) 685
FspBI CTAG 9 cut(s) 315, 716, 920, 1130, 1641, 1835, 1910, 2150, 2193
GlaI GCGC 2 cut(s) 96, 535
GsaI CCCAGC 1 cut(s) 1137
GsuI CTGGAG 4 cut(s) 873, 1265, 1398, 1644
HaeII RGCGCY 2 cut(s) 98, 537
HaeIII GGCC 7 cut(s) 144, 217, 662, 1674, 1680, 1928, 2043
HapII CCGG 4 cut(s) 89, 998, 1481, 1761
HgaI GACGC 1 cut(s) 2190
HhaI GCGC 2 cut(s) 97, 536
Hin6I GCGC 2 cut(s) 95, 534
HinP1I GCGC 2 cut(s) 95, 534
HincII GTYRAC 4 cut(s) 1620, 1668, 2490, 2764
HindII GTYRAC 4 cut(s) 1620, 1668, 2490, 2764
HpaII CCGG 4 cut(s) 89, 998, 1481, 1761
HphI GGTGA 1 cut(s) 2465
Hpy166II GTNNAC 8 cut(s) 1124, 1620, 1668, 2177, 2490, 2764, 2770, 2822
Hpy8I GTNNAC 8 cut(s) 1124, 1620, 1668, 2177, 2490, 2764, 2770, 2822
Hpy99I CGWCG 1 cut(s) 1944
HpyCH4III ACNGT 8 cut(s) 202, 418, 1010, 1298, 1452, 1983, 2304, 2792
HpyCH4IV ACGT 6 cut(s) 57, 774, 1756, 1939, 2173, 2766
HpySE526I ACGT 6 cut(s) 57, 774, 1756, 1939, 2173, 2766
HspAI GCGC 2 cut(s) 95, 534
Kpn2I TCCGGA 1 cut(s) 1480
Ksp22I TGATCA 2 cut(s) 571, 2320
LmnI GCTCC 9 cut(s) 529, 680, 688, 1000, 1111, 2006, 2515, 2523, 2601
LweI GCATC 6 cut(s) 908, 1029, 1552, 1669, 1747, 2197
MaeI CTAG 9 cut(s) 315, 716, 920, 1130, 1641, 1835, 1910, 2150, 2193
MaeII ACGT 6 cut(s) 57, 774, 1756, 1939, 2173, 2766
MaeIII GTNAC 4 cut(s) 908, 1298, 1983, 2678
MbiI CCGCTC 1 cut(s) 2518
MfeI CAATTG 2 cut(s) 1227, 2591
MflI RGATCY 2 cut(s) 478, 832
MhlI GDGCHC 1 cut(s) 685
MlsI TGGCCA 1 cut(s) 1674
MluNI TGGCCA 1 cut(s) 1674
MlyI GAGTC 6 cut(s) 575, 738, 1382, 1625, 2686, 2790
Mox20I TGGCCA 1 cut(s) 1674
Mph1103I ATGCAT 1 cut(s) 1691
MroI TCCGGA 1 cut(s) 1480
MroXI GAANNNNTTC 2 cut(s) 1878, 2100
MscI TGGCCA 1 cut(s) 1674
MseI TTAA 5 cut(s) 278, 459, 1530, 1593, 2163
MslI CAYNNNNRTG 1 cut(s) 2328
Msp20I TGGCCA 1 cut(s) 1674
MspA1I CMGCKG 2 cut(s) 1202, 1286
MspCI CTTAAG 1 cut(s) 458
MspI CCGG 4 cut(s) 89, 998, 1481, 1761
MspR9I CCNGG 6 cut(s) 89, 138, 147, 256, 998, 2729
MunI CAATTG 2 cut(s) 1227, 2591
Mva1269I GAATGC 1 cut(s) 1585
MvaI CCWGG 4 cut(s) 138, 147, 256, 2729
MvnI CGCG 2 cut(s) 2224, 2497
NciI CCSGG 2 cut(s) 89, 998
NcoI CCATGG 2 cut(s) 1675, 1782
NmuCI GTSAC 2 cut(s) 1298, 2678
NruI TCGCGA 2 cut(s) 2224, 2497
NsiI ATGCAT 1 cut(s) 1691
NspI RCATGY 4 cut(s) 116, 842, 1538, 1693
PaeI GCATGC 1 cut(s) 1693
PaqCI CACCTGC 1 cut(s) 1622
PciI ACATGT 2 cut(s) 838, 1534
PcsI WCGNNNNNNNCGW 1 cut(s) 442
PctI GAATGC 1 cut(s) 1585
PdmI GAANNNNTTC 2 cut(s) 1878, 2100
PfeI GAWTC 8 cut(s) 173, 744, 779, 923, 960, 1069, 1787, 2405
Pfl23II CGTACG 1 cut(s) 974
PflFI GACNNNGTC 1 cut(s) 2229
PflMI CCANNNNNTGG 1 cut(s) 2578
PfoI TCCNGGA 4 cut(s) 87, 254, 996, 2727
PinAI ACCGGT 1 cut(s) 1760
PleI GAGTC 6 cut(s) 575, 737, 1381, 1624, 2685, 2789
PpsI GAGTC 6 cut(s) 575, 737, 1381, 1624, 2685, 2789
PscI ACATGT 2 cut(s) 838, 1534
PshAI GACNNNNGTC 1 cut(s) 2775
PsiI TTATAA 1 cut(s) 1235
Psp124BI GAGCTC 1 cut(s) 685
Psp1406I AACGTT 1 cut(s) 57
Psp6I CCWGG 4 cut(s) 136, 145, 254, 2727
PspFI CCCAGC 1 cut(s) 1133
PspGI CCWGG 4 cut(s) 136, 145, 254, 2727
PspLI CGTACG 1 cut(s) 974
PspPI GGNCC 6 cut(s) 143, 215, 601, 1718, 2041, 2284
PsrI GAACNNNNNNTAC 2 cut(s) 1863, 1895
PstI CTGCAG 3 cut(s) 763, 1210, 2416
PstNI CAGNNNCTG 2 cut(s) 896, 1205
PsuI RGATCY 2 cut(s) 478, 832
PsyI GACNNNGTC 1 cut(s) 2229
PvuII CAGCTG 1 cut(s) 1202
RruI TCGCGA 2 cut(s) 2224, 2497
RsaI GTAC 8 cut(s) 286, 420, 976, 1352, 1759, 2049, 2122, 2467
RsaNI GTAC 8 cut(s) 285, 419, 975, 1351, 1758, 2048, 2121, 2466
RseI CAYNNNNRTG 1 cut(s) 2328
SacI GAGCTC 1 cut(s) 685
SalI GTCGAC 1 cut(s) 1618
SaqAI TTAA 5 cut(s) 278, 459, 1530, 1593, 2163
Sau96I GGNCC 6 cut(s) 143, 215, 601, 1718, 2041, 2284
ScaI AGTACT 1 cut(s) 1352
SchI GAGTC 6 cut(s) 575, 738, 1382, 1625, 2686, 2790
ScrFI CCNGG 6 cut(s) 89, 138, 147, 256, 998, 2729
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 6 cut(s) 908, 1029, 1552, 1669, 1747, 2197
SfcI CTRYAG 4 cut(s) 759, 1206, 1973, 2412
SinI GGWCC 3 cut(s) 601, 1718, 2284
SmiMI CAYNNNNRTG 1 cut(s) 2328
SmlI CTYRAG 3 cut(s) 458, 1221, 2090
SmoI CTYRAG 3 cut(s) 458, 1221, 2090
SphI GCATGC 1 cut(s) 1693
SsiI CCGC 7 cut(s) 134, 141, 1284, 1925, 2394, 2518, 2521
SspMI CTAG 9 cut(s) 315, 716, 920, 1130, 1641, 1835, 1910, 2150, 2193
SstI GAGCTC 1 cut(s) 685
StyD4I CCNGG 6 cut(s) 87, 136, 145, 254, 996, 2727
StyI CCWWGG 6 cut(s) 352, 663, 1473, 1675, 1782, 2837
TaaI ACNGT 8 cut(s) 202, 418, 1010, 1298, 1452, 1983, 2304, 2792
TaiI ACGT 6 cut(s) 60, 777, 1759, 1942, 2176, 2769
TaqI TCGA 5 cut(s) 784, 1072, 1149, 1619, 2779
TatI WGTACW 3 cut(s) 418, 1350, 2465
TauI GCSGC 3 cut(s) 144, 1928, 2521
TfiI GAWTC 8 cut(s) 173, 744, 779, 923, 960, 1069, 1787, 2405
Tru1I TTAA 5 cut(s) 278, 459, 1530, 1593, 2163
Tru9I TTAA 5 cut(s) 278, 459, 1530, 1593, 2163
TscAI CASTG 9 cut(s) 388, 507, 921, 1303, 1420, 1432, 2286, 2421, 2570
TseFI GTSAC 2 cut(s) 1298, 2678
Tsp45I GTSAC 2 cut(s) 1298, 2678
TspRI CASTG 9 cut(s) 388, 507, 921, 1303, 1420, 1432, 2286, 2421, 2570
Tth111I GACNNNGTC 1 cut(s) 2229
Van91I CCANNNNNTGG 1 cut(s) 2578
Vha464I CTTAAG 1 cut(s) 458
VpaK11BI GGWCC 3 cut(s) 601, 1718, 2284
XapI RAATTY 3 cut(s) 343, 1026, 2541
XbaI TCTAGA 2 cut(s) 1640, 1909
XceI RCATGY 4 cut(s) 116, 842, 1538, 1693
XcmI CCANNNNNNNNNTGG 1 cut(s) 134
XmiI GTMKAC 1 cut(s) 1619
XmnI GAANNNNTTC 2 cut(s) 1878, 2100
XspI CTAG 9 cut(s) 315, 716, 920, 1130, 1641, 1835, 1910, 2150, 2193
ZrmI AGTACT 1 cut(s) 1352
Zsp2I ATGCAT 1 cut(s) 1691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.