MD08G1195300.v1.1

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007329
Physical Location & Seq
Forward (+)
25194384 .. 25208726
14343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1195300.v1.1.491

Sequence Viewer

Length: 2790 bp
ATGACTAACCGAATCACCAATGCGCCTCAGGGGGAAGGCGATGCTCCTCCTCCTCCTCCTCCTCCCCCATCTGGTGGCCCTGGCCCACCTGAATCTCAGCCTTCCCGTGGTGCCAACACGATATTCAAGAGTGGACCGTTATTCATATCATCCAAAGGAATTGGGTGGACGTCCTGGAAGAAAAGATGGTTTATCCTAACTCGCACATCACTCGTTTTCTTCAGAAGTGATCCAAGTGCTGCTCTAAAGGGGAGTGAAGTGAACTTGACCCTGGGTGGTATTGACCTCAACAATTCAGGCAGTGTGGTTGTCAAAGCAGACAAGAAACTCTTGACCGTGCTCTTTCCAGATGGTCGTGATGGACGAGCCTTCACACTTAAGGCTGAAACATTGGAAGATCTACATGAGTGGAAGACTGCACTTGAAAATGCGTTGGCGCAAGCCCCAAGTGGTGGCGGTCATGCTATGGGGCAAAATGGAACGTTAGGGAATGATAAGACTCAGGCTGTTGATGGTTCTGCGGACCAGTCAAAGGATAAGAAGCCAGTGAAATCTACAGTCGTTGGAGTGCCTGTTCTACTGGCCTTGGAAGATGTTGATGGAGCTCCGTCTTTCTTGGAAAAAGCCTTTAGGTTCGTAGAAGAATATGGAGTCAAAGTAGAAGGGATCTTACGACAAGCTGCGGATGTTGAAGATGTTCAAAGTAGAATAAGAGAGTATGAACAGGGGAAAACTGAGTTTTCTCCTCAGGAGGACGCACATGTTATCGCTGATTGTGTCAAGTATGTCCTCCGGGAGTTGCCATCTCCCCCTGTCCCTGCATCTTGTTGTAATGCACTGTTAGAAGCCTTTCGTAAATCTGGTAATGATCGTGGTGGTAGAGTCAATGCTATGCGCACAACAATATGTGATACATTCCCAGAGCCAAATCGCCGTTTACTGCAAAGAATTCTTTTGATGATGCAAAGTGTGGCTGCTCAGAAAGCTGTGAACCGAATGAGCTGCTCTGCTGTGGCAGCTTGTATGGCTCCTTTACTTCTTCGCCCGCTCTTAGCTGGGGATTGTGAAATTGACAATGATTTTGATGTGGGGGGTGATGGTTCTGTTCAACTATTGCAAGCTGCTGCTGCCGCTAATCATGCTCAAGCGATTGTTATAACTTTGTTGGAGGAGTATAATCACATATTTGGGGAAGGTGGTCCCCTGTCCCCGGAACTATACAGTGACACAGAAGAAAGTGAAAGTGAGACTGAGGGGGCAACAGATGATGATGATTACTATGAAGATGATGAAACTGATGCAGGAACAGAGGATGAAGATGTTGAAATTGTTTCTGATGGAGATGGAACATTTAGTGAGAGTGGTGAATCTGAAGACAATGATCTGCATGATAATAAGGATGGTGGTGACGGTTTAAGACCAGGAGTGAAGTCTCCTCATGGAGGCAACGATGGTAAAGCTCATGAGAAGTTGTCATCAAGCTCAACTAAAATATTGCAGCCTCAACCTGCTTTAGAGAGCAGTGAGAATAAACTGAGTTCAAGTAAAACTAATGCGCCAGAGCAGGCTAGCGAGTCTGCTGTAGTAGAAGAGGTTTCTAGAGAAATAAGTTCACGGCAACAGCCACCCAATCATGTTCTTCCATCCCCATTTATACAAAAATCTTCATCTATATCCAATGGATCTGGGCATGTTAGGAATCGTACTGGTTGGGGACGTATGGCTGCAAGGAAGAACCTTTCCATGGAATCCATTGACTACAGTCTTGAGGAAGAGGCTGAAATCCAGAATCTTGAGAGGACTAAATCTGAGTTAGAAAACAGAATTGCAGAAGAGGTTGAAGGGAATGCTGCTCTACAAGCAAGTCTGGAGAGAGAGAAGACGGCCTTGCGTGAGCGTCGCCTAGCTCTCAAGCAAGATGTGGCGAGACTACAGGAACAGTTACAGAAGGAGAAGGATTTAACAGCAGCTCTCGAAGCAGGACTAAAAATGTCGGGGGGACATGTGCCCAATTTAGCCACAGTTGATGAAAAAACAAGGGCAGATCTTATTGAAATAGCTCAGGCAGAAGAGAATGTTTCCAACTTGAAGATGAAGGTTGATGATCTAGGCATGCAGCTTAATCAACAACGTGAAAGAAACCGTGGCTTTATGTCTGATGCTTCCATTCCATCCCGAAATAGTGGCGATCATCAAGCAAAACAGCACACAACAGATAGACAGAAGGACAGTGAAAACATAGTCCCTTCCCATTTCGAATCGTCAAGAAGCAAGGATACTCATCAAGATGGAACGGAAAATGTGAATGGAAAGAAAACAGATGCAGCAAACAAAAGCAACTCTCGGTCTGTTGGGCCGCCAGCTGATTCTTCTGCAGTAGAGACAGTGGTAGCCAAGCCTACAGCTTCAACCAACTCCAAGAAAGGCGGTGGGGGTGAGGGAGGTAATTCCACAACTTCAGCAATAACAAAGTTGACATCACGACTGAATTTGTTGAAGGAGCGGCGGAGCCAAATAGCAAATGAGATTCAAGGCCGCGGTCCTGGTCAAAGTTTGGATAAAAGTCAGTCGCTCCAATACGACAAGAGTTATGAAACCTCGGAAAAGCCAGAGAAACTTAGGAAGGCGGAGAGTCATTCGGAGAGAGGAGGAAAGAGATCAGAAAGCCAGCAGCAGCAGCATAGCCTGGACAGAGGAAAATCGGAAAGCCATCTATCTGTAGCCGTGGAGAAGGGTCGAGTTGTAGAGGGTAGTAAACTGAAAACTTCTCCGAGGACGGACAGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

930

Amino Acids

100.4

Weight (kDa)

5.23

Isoelectric Point (pI)

56.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 43 - 145 1.2e-11 PH domain
RhoGAP PF00620 203 - 349 1.9e-29 RhoGAP domain
Lzipper-MIP1 PF14389 630 - 710 1.1e-18 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
AatII GACGTC 1 cut(s) 173
Acc16I TGCGCA 1 cut(s) 896
Acc36I ACCTGC 1 cut(s) 1516
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 2 cut(s) 74, 452
AccBSI CCGCTC 2 cut(s) 1048, 2503
AccII CGCG 1 cut(s) 2538
AclI AACGTT 1 cut(s) 482
AclWI GGATC 3 cut(s) 224, 674, 1690
AcsI RAATTY 2 cut(s) 948, 2488
AcuI CTGAAG 3 cut(s) 205, 1392, 2442
AcyI GRCGYC 1 cut(s) 170
AfaI GTAC 1 cut(s) 1705
AfiI CCNNNNNNNGG 8 cut(s) 71, 74, 107, 452, 532, 1210, 1442, 1744
AflII CTTAAG 1 cut(s) 377
AflIII ACRYGT 2 cut(s) 760, 2002
AjnI CCWGG 6 cut(s) 79, 173, 270, 1420, 2542, 2685
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
AloI GAACNNNNNNTCC 2 cut(s) 558, 590
Alw21I GWGCWC 2 cut(s) 342, 607
Alw26I GTCTC 4 cut(s) 1241, 1437, 1921, 2375
AlwI GGATC 3 cut(s) 224, 674, 1690
AoxI GGCC 6 cut(s) 76, 82, 582, 1884, 2354, 2533
ApoI RAATTY 2 cut(s) 948, 2488
ArsI GACNNNNNNTTYG 2 cut(s) 1064, 1096
Asp700I GAANNNNTTC 2 cut(s) 696, 849
AspLEI GCGC 4 cut(s) 25, 439, 897, 1558
AspS9I GGNCC 7 cut(s) 77, 83, 134, 523, 1199, 2354, 2540
AsuC2I CCSGG 2 cut(s) 794, 1211
AsuHPI GGTGA 5 cut(s) 7, 1106, 1376, 1418, 2447
AsuII TTCGAA 1 cut(s) 2256
AsuNHI GCTAGC 1 cut(s) 1568
AvaII GGWCC 4 cut(s) 134, 523, 1199, 2540
AxyI CCTNAGG 2 cut(s) 27, 747
BaeGI GKGCMC 1 cut(s) 2010
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 607
BarI GAAGNNNNNNTAC 4 cut(s) 654, 686, 2260, 2292
BbsI GAAGAC 3 cut(s) 419, 1380, 1886
Bbv12I GWGCWC 2 cut(s) 342, 607
BceAI ACGGC 4 cut(s) 918, 1631, 1899, 2708
BcgI CGANNNNNNTGC 4 cut(s) 193, 227, 984, 1018
BciT130I CCWGG 6 cut(s) 81, 175, 272, 1422, 2544, 2687
BciVI GTATCC 1 cut(s) 2269
BcnI CCSGG 2 cut(s) 794, 1211
BcoDI GTCTC 4 cut(s) 1241, 1437, 1921, 2375
BfaI CTAG 4 cut(s) 1569, 1599, 1904, 2108
BfmI CTRYAG 7 cut(s) 555, 1581, 1759, 1931, 2373, 2400, 2718
BfrI CTTAAG 1 cut(s) 377
BfuAI ACCTGC 1 cut(s) 1516
BfuI GTATCC 1 cut(s) 2269
BglII AGATCT 2 cut(s) 397, 2044
Bme1390I CCNGG 8 cut(s) 81, 175, 272, 794, 1211, 1422, 2544, 2687
Bme18I GGWCC 4 cut(s) 134, 523, 1199, 2540
BmgT120I GGNCC 7 cut(s) 77, 83, 134, 523, 1199, 2354, 2540
BmiI GGNNCC 4 cut(s) 112, 1029, 1201, 2510
BmrFI CCNGG 8 cut(s) 81, 175, 272, 794, 1211, 1422, 2544, 2687
BmsI GCATC 6 cut(s) 31, 830, 951, 1288, 2149, 2311
BmtI GCTAGC 1 cut(s) 1572
BoxI GACNNNNGTC 1 cut(s) 1761
BpiI GAAGAC 3 cut(s) 419, 1380, 1886
BpmI CTGGAG 1 cut(s) 1889
Bpu10I CCTNAGC 1 cut(s) 2061
Bpu14I TTCGAA 1 cut(s) 2256
BpuEI CTTGAG 4 cut(s) 1128, 1787, 1814, 1895
BpuMI CCSGG 2 cut(s) 794, 1211
BsaBI GATNNNNATC 1 cut(s) 2280
BsaHI GRCGYC 1 cut(s) 170
BsaXI ACNNNNNCTCC 4 cut(s) 558, 588, 642, 672
Bsc4I CCNNNNNNNGG 8 cut(s) 71, 74, 107, 452, 532, 1210, 1442, 1744
Bse1I ACTGG 4 cut(s) 526, 545, 585, 1711
Bse21I CCTNAGG 2 cut(s) 27, 747
Bse8I GATNNNNATC 1 cut(s) 2280
BseBI CCWGG 6 cut(s) 81, 175, 272, 1422, 2544, 2687
BseGI GGATG 6 cut(s) 149, 691, 1318, 1405, 1643, 2171
BseJI GATNNNNATC 1 cut(s) 2280
BseLI CCNNNNNNNGG 8 cut(s) 71, 74, 107, 452, 532, 1210, 1442, 1744
BseNI ACTGG 4 cut(s) 526, 545, 585, 1711
BseSI GKGCMC 1 cut(s) 2010
BseYI CCCAGC 1 cut(s) 1055
BsgI GTGCAG 1 cut(s) 402
Bsh1236I CGCG 1 cut(s) 2538
BshFI GGCC 6 cut(s) 78, 84, 584, 1886, 2356, 2535
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 2 cut(s) 342, 607
BsiSI CCGG 2 cut(s) 793, 1211
BslFI GGGAC 6 cut(s) 800, 1185, 1192, 1728, 2013, 2228
BslI CCNNNNNNNGG 8 cut(s) 71, 74, 107, 452, 532, 1210, 1442, 1744
BsmAI GTCTC 4 cut(s) 1241, 1437, 1921, 2375
BsmFI GGGAC 6 cut(s) 800, 1185, 1192, 1728, 2013, 2228
BsmI GAATGC 1 cut(s) 1852
BsnI GGCC 6 cut(s) 78, 84, 584, 1886, 2356, 2535
Bsp119I TTCGAA 1 cut(s) 2256
Bsp1286I GDGCHC 3 cut(s) 342, 607, 2010
Bsp19I CCATGG 1 cut(s) 1743
BspANI GGCC 6 cut(s) 78, 84, 584, 1886, 2356, 2535
BspFNI CGCG 1 cut(s) 2538
BspHI TCATGA 1 cut(s) 1462
BspLI GGNNCC 4 cut(s) 112, 1029, 1201, 2510
BspMAI CTGCAG 1 cut(s) 2377
BspMI ACCTGC 1 cut(s) 1516
BspOI GCTAGC 1 cut(s) 1572
BspPI GGATC 3 cut(s) 224, 674, 1690
BspT104I TTCGAA 1 cut(s) 2256
BspT107I GGYRCC 1 cut(s) 110
BspTI CTTAAG 1 cut(s) 377
BsrBI CCGCTC 2 cut(s) 1048, 2503
BsrI ACTGG 4 cut(s) 526, 545, 585, 1711
BssNI GRCGYC 1 cut(s) 170
BssT1I CCWWGG 2 cut(s) 585, 1743
Bst2UI CCWGG 6 cut(s) 81, 175, 272, 1422, 2544, 2687
Bst6I CTCTTC 4 cut(s) 1584, 1767, 1827, 2064
BstACI GRCGYC 1 cut(s) 170
BstAFI CTTAAG 1 cut(s) 377
BstBI TTCGAA 1 cut(s) 2256
BstC8I GCNNGC 8 cut(s) 441, 1046, 1119, 1566, 1570, 2114, 2361, 2669
BstDSI CCRYGG 5 cut(s) 106, 1743, 2143, 2536, 2724
BstENI CCTNNNNNAGG 1 cut(s) 1440
BstF5I GGATG 6 cut(s) 149, 691, 1318, 1405, 1643, 2171
BstFNI CGCG 1 cut(s) 2538
BstHHI GCGC 4 cut(s) 25, 439, 897, 1558
BstMAI GTCTC 4 cut(s) 1241, 1437, 1921, 2375
BstMWI GCNNNNNNNGC 9 cut(s) 983, 1016, 1025, 1127, 1130, 1139, 1859, 1976, 2677
BstNI CCWGG 6 cut(s) 81, 175, 272, 1422, 2544, 2687
BstNSI RCATGY 4 cut(s) 764, 1694, 2006, 2116
BstPAI GACNNNNGTC 1 cut(s) 1761
BstSCI CCNGG 8 cut(s) 79, 173, 270, 792, 1209, 1420, 2542, 2685
BstSFI CTRYAG 7 cut(s) 555, 1581, 1759, 1931, 2373, 2400, 2718
BstSLI GKGCMC 1 cut(s) 2010
BstUI CGCG 1 cut(s) 2538
BstV2I GAAGAC 3 cut(s) 419, 1380, 1886
BstX2I RGATCY 4 cut(s) 397, 666, 1682, 2044
BstYI RGATCY 4 cut(s) 397, 666, 1682, 2044
Bsu36I CCTNAGG 2 cut(s) 27, 747
BsuI GTATCC 1 cut(s) 2269
BsuRI GGCC 6 cut(s) 78, 84, 584, 1886, 2356, 2535
BtgI CCRYGG 5 cut(s) 106, 1743, 2143, 2536, 2724
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 6 cut(s) 149, 691, 1318, 1405, 1643, 2171
BtsI GCAGTG 2 cut(s) 307, 1528
BtsIMutI CAGTG 7 cut(s) 307, 552, 836, 1228, 1528, 2236, 2391
BveI ACCTGC 1 cut(s) 1516
Cac8I GCNNGC 8 cut(s) 441, 1046, 1119, 1566, 1570, 2114, 2361, 2669
CciI TCATGA 1 cut(s) 1462
CfoI GCGC 4 cut(s) 25, 439, 897, 1558
Cfr13I GGNCC 7 cut(s) 77, 83, 134, 523, 1199, 2354, 2540
Cfr42I CCGCGG 1 cut(s) 2539
CseI GACGC 2 cut(s) 764, 1886
Csp6I GTAC 1 cut(s) 1704
CviQI GTAC 1 cut(s) 1704
Eam1104I CTCTTC 4 cut(s) 1584, 1767, 1827, 2064
EarI CTCTTC 4 cut(s) 1584, 1767, 1827, 2064
EciI GGCGGA 2 cut(s) 2521, 2642
Ecl136II GAGCTC 1 cut(s) 605
Eco130I CCWWGG 2 cut(s) 585, 1743
Eco24I GRGCYC 1 cut(s) 607
Eco47I GGWCC 4 cut(s) 134, 523, 1199, 2540
Eco53kI GAGCTC 1 cut(s) 605
Eco57I CTGAAG 3 cut(s) 205, 1392, 2442
Eco81I CCTNAGG 2 cut(s) 27, 747
EcoICRI GAGCTC 1 cut(s) 605
EcoNI CCTNNNNNAGG 1 cut(s) 1440
EcoRI GAATTC 1 cut(s) 948
EcoRII CCWGG 6 cut(s) 79, 173, 270, 1420, 2542, 2685
EcoT14I CCWWGG 2 cut(s) 585, 1743
EcoT38I GRGCYC 1 cut(s) 607
ErhI CCWWGG 2 cut(s) 585, 1743
FalI AAGNNNNNCTT 4 cut(s) 314, 346, 1871, 1903
FaqI GGGAC 6 cut(s) 800, 1185, 1192, 1728, 2013, 2228
FauI CCCGC 1 cut(s) 1053
FokI GGATG 6 cut(s) 136, 698, 1325, 1412, 1630, 2158
FriOI GRGCYC 1 cut(s) 607
FspAI RTGCGCAY 1 cut(s) 896
FspBI CTAG 4 cut(s) 1569, 1599, 1904, 2108
FspI TGCGCA 1 cut(s) 896
GlaI GCGC 4 cut(s) 24, 438, 896, 1557
GsaI CCCAGC 1 cut(s) 1059
GsuI CTGGAG 1 cut(s) 1889
HaeIII GGCC 6 cut(s) 78, 84, 584, 1886, 2356, 2535
HapII CCGG 2 cut(s) 793, 1211
HgaI GACGC 2 cut(s) 764, 1886
HhaI GCGC 4 cut(s) 25, 439, 897, 1558
Hin1I GRCGYC 1 cut(s) 170
Hin6I GCGC 4 cut(s) 23, 437, 895, 1556
HinP1I GCGC 4 cut(s) 23, 437, 895, 1556
HincII GTYRAC 1 cut(s) 2475
HindII GTYRAC 1 cut(s) 2475
HpaII CCGG 2 cut(s) 793, 1211
HphI GGTGA 5 cut(s) 7, 1106, 1376, 1418, 2447
Hpy166II GTNNAC 8 cut(s) 134, 168, 262, 938, 991, 1613, 2475, 2756
Hpy8I GTNNAC 8 cut(s) 134, 168, 262, 938, 991, 1613, 2475, 2756
Hpy99I CGWCG 1 cut(s) 1902
HpyCH4IV ACGT 4 cut(s) 170, 482, 1717, 2131
HpyF10VI GCNNNNNNNGC 9 cut(s) 983, 1016, 1025, 1127, 1130, 1139, 1859, 1976, 2677
HpySE526I ACGT 4 cut(s) 170, 482, 1717, 2131
Hsp92I GRCGYC 1 cut(s) 170
HspAI GCGC 4 cut(s) 23, 437, 895, 1556
KspI CCGCGG 1 cut(s) 2539
LmnI GCTCC 7 cut(s) 49, 602, 610, 1033, 2500, 2508, 2577
LweI GCATC 6 cut(s) 31, 830, 951, 1288, 2149, 2311
MaeI CTAG 4 cut(s) 1569, 1599, 1904, 2108
MaeII ACGT 4 cut(s) 170, 482, 1717, 2131
MaeIII GTNAC 3 cut(s) 1223, 1406, 1941
MbiI CCGCTC 2 cut(s) 1048, 2503
MflI RGATCY 4 cut(s) 397, 666, 1682, 2044
MhlI GDGCHC 3 cut(s) 342, 607, 2010
MluCI AATT 9 cut(s) 159, 292, 948, 1068, 1326, 1824, 2011, 2446, 2488
MlyI GAGTC 5 cut(s) 493, 660, 891, 1583, 2641
MmeI TCCRAC 3 cut(s) 544, 1146, 2106
MroXI GAANNNNTTC 2 cut(s) 696, 849
MseI TTAA 4 cut(s) 378, 1415, 1961, 2121
MslI CAYNNNNRTG 1 cut(s) 2286
MspA1I CMGCKG 2 cut(s) 2363, 2538
MspCI CTTAAG 1 cut(s) 377
MspI CCGG 2 cut(s) 793, 1211
MspR9I CCNGG 8 cut(s) 81, 175, 272, 794, 1211, 1422, 2544, 2687
Mva1269I GAATGC 1 cut(s) 1852
MvaI CCWGG 6 cut(s) 81, 175, 272, 1422, 2544, 2687
MvnI CGCG 1 cut(s) 2538
MwoI GCNNNNNNNGC 9 cut(s) 983, 1016, 1025, 1127, 1130, 1139, 1859, 1976, 2677
NciI CCSGG 2 cut(s) 794, 1211
NcoI CCATGG 1 cut(s) 1743
NheI GCTAGC 1 cut(s) 1568
NlaIV GGNNCC 4 cut(s) 112, 1029, 1201, 2510
NmuCI GTSAC 2 cut(s) 1223, 1406
NsbI TGCGCA 1 cut(s) 896
NspI RCATGY 4 cut(s) 764, 1694, 2006, 2116
NspV TTCGAA 1 cut(s) 2256
PaeI GCATGC 1 cut(s) 2116
PagI TCATGA 1 cut(s) 1462
PasI CCCWGGG 1 cut(s) 271
PciI ACATGT 2 cut(s) 760, 2002
PcsI WCGNNNNNNNCGW 1 cut(s) 361
PctI GAATGC 1 cut(s) 1852
PdmI GAANNNNTTC 2 cut(s) 696, 849
PfeI GAWTC 9 cut(s) 12, 92, 1367, 1699, 1748, 1789, 2258, 2366, 2527
PflMI CCANNNNNTGG 2 cut(s) 74, 452
PfoI TCCNGGA 2 cut(s) 173, 792
PleI GAGTC 5 cut(s) 493, 659, 890, 1582, 2640
PpsI GAGTC 5 cut(s) 493, 659, 890, 1582, 2640
PscI ACATGT 2 cut(s) 760, 2002
PshAI GACNNNNGTC 1 cut(s) 1761
PsiI TTATAA 1 cut(s) 1157
Psp124BI GAGCTC 1 cut(s) 607
Psp1406I AACGTT 1 cut(s) 482
Psp6I CCWGG 6 cut(s) 79, 173, 270, 1420, 2542, 2685
PspFI CCCAGC 1 cut(s) 1055
PspGI CCWGG 6 cut(s) 79, 173, 270, 1420, 2542, 2685
PspN4I GGNNCC 4 cut(s) 112, 1029, 1201, 2510
PspPI GGNCC 7 cut(s) 77, 83, 134, 523, 1199, 2354, 2540
PstI CTGCAG 1 cut(s) 2377
PsuI RGATCY 4 cut(s) 397, 666, 1682, 2044
PvuII CAGCTG 1 cut(s) 2363
RsaI GTAC 1 cut(s) 1705
RsaNI GTAC 1 cut(s) 1704
RseI CAYNNNNRTG 1 cut(s) 2286
SacI GAGCTC 1 cut(s) 607
SacII CCGCGG 1 cut(s) 2539
SaqAI TTAA 4 cut(s) 378, 1415, 1961, 2121
Sau96I GGNCC 7 cut(s) 77, 83, 134, 523, 1199, 2354, 2540
SchI GAGTC 5 cut(s) 493, 660, 891, 1583, 2641
ScrFI CCNGG 8 cut(s) 81, 175, 272, 794, 1211, 1422, 2544, 2687
SduI GDGCHC 3 cut(s) 342, 607, 2010
SfaNI GCATC 6 cut(s) 31, 830, 951, 1288, 2149, 2311
SfcI CTRYAG 7 cut(s) 555, 1581, 1759, 1931, 2373, 2400, 2718
Sfr303I CCGCGG 1 cut(s) 2539
SfuI TTCGAA 1 cut(s) 2256
SgrBI CCGCGG 1 cut(s) 2539
SinI GGWCC 4 cut(s) 134, 523, 1199, 2540
SmiMI CAYNNNNRTG 1 cut(s) 2286
SmlI CTYRAG 5 cut(s) 377, 1143, 1766, 1793, 1910
SmoI CTYRAG 5 cut(s) 377, 1143, 1766, 1793, 1910
SphI GCATGC 1 cut(s) 2116
Sse9I AATT 9 cut(s) 159, 292, 948, 1068, 1326, 1824, 2011, 2446, 2488
SspI AATATT 1 cut(s) 1494
SspMI CTAG 4 cut(s) 1569, 1599, 1904, 2108
SstI GAGCTC 1 cut(s) 607
StyD4I CCNGG 8 cut(s) 79, 173, 270, 792, 1209, 1420, 2542, 2685
StyI CCWWGG 2 cut(s) 585, 1743
TaiI ACGT 4 cut(s) 173, 485, 1720, 2134
TaqI TCGA 3 cut(s) 1974, 2256, 2737
TaqII GACCGA 1 cut(s) 2334
TasI AATT 9 cut(s) 159, 292, 948, 1068, 1326, 1824, 2011, 2446, 2488
TauI GCSGC 4 cut(s) 1133, 2359, 2506, 2538
TfiI GAWTC 9 cut(s) 12, 92, 1367, 1699, 1748, 1789, 2258, 2366, 2527
Tru1I TTAA 4 cut(s) 378, 1415, 1961, 2121
Tru9I TTAA 4 cut(s) 378, 1415, 1961, 2121
TscAI CASTG 7 cut(s) 307, 552, 843, 1228, 1528, 2236, 2391
TseFI GTSAC 2 cut(s) 1223, 1406
Tsp45I GTSAC 2 cut(s) 1223, 1406
TspDTI ATGAA 9 cut(s) 133, 735, 1296, 1305, 1329, 1656, 2043, 2108, 2607
TspGWI ACGGA 2 cut(s) 597, 2309
TspRI CASTG 7 cut(s) 307, 552, 843, 1228, 1528, 2236, 2391
Van91I CCANNNNNTGG 2 cut(s) 74, 452
Vha464I CTTAAG 1 cut(s) 377
VpaK11BI GGWCC 4 cut(s) 134, 523, 1199, 2540
XagI CCTNNNNNAGG 1 cut(s) 1440
XapI RAATTY 2 cut(s) 948, 2488
XbaI TCTAGA 1 cut(s) 1598
XceI RCATGY 4 cut(s) 764, 1694, 2006, 2116
XmnI GAANNNNTTC 2 cut(s) 696, 849
XspI CTAG 4 cut(s) 1569, 1599, 1904, 2108
ZraI GACGTC 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.