Rh7DG445400

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0007329
Physical Location & Seq
Reverse (-)
63185034 .. 63197403
12370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG445400.1

Sequence Viewer

Length: 2769 bp
ATGACTAACATAATCCCGGCAGCGCCTCAGGGGGATGGTCATGCTTCTCCTCCCCAACCCCCGCCTGGCGGCCCTGGTGGCGACCCCGATCATCATGCTGAATCTCATGGTTCTCGTGGTGGCAATACGGTTTTCAAGAGTGGCCCACTATTTATATCTTCCAAAGGAATTGGATGGACATCCTGGAAAAAGAGATGGTTTATTTTAACTCGTACTTCGCTGGTTTTCTTCAGAAGTGATCCTAGTGCTGTTCAGAAGGGGAGTGAAGTGAATTTGACCCTTGGTGGTATTGACCTCAACAATTCAGGCAGTGTGGTTGTCAAAGAAGACAAGAAACTCTTGACTGTACTCTTTCCAGATGGTCGTGATGGGCGAGCCTTCACACTTAAGGCTGAAACTTCGAAAGATCTACATGAATGGAAGGAAGCACTGGATAACGCTTTGGCACAAGCTCCAAGTGGCGCTCATGGTGTGAGGCAAAATGGTATCTTGGGGAATAATCAGACAGACTCCAAAAATGGTTCTTTGGACCAGCCAAAGGAGAAACCACCTGTGAAATCTACAATTATTGGGGTGCCTATTTTGCTGGCCTTGGAAGATGTTGATGGAGCTCCATGTTTCTTGGAAAAAGCCCTTAGGTTCCTAGAAGAACATGGAGTCAAGGTGGAAGGAATCTTGCGGCAAGCTGCAGATGTTGATGATGTTGAATCTCGAATACGGGAATATGAGAAGGGGAAAGTTGATTTTTCTCCCGAGGAGGATCCACATGTCATTGCTGATTGTGTCAAGTATGTCCTCCGAGAGTTGCCATCTTCTCCAGTCCCTGCATCTTGCTGTAACGCACTGCTAGATTCCTTTCGCAAGTCTGGTAATGATCGGGGTGGTAGAATCAATGCCATGCGTACGACAATATGTGATACATTCCCGGAACCAAACCGTCGTTTATTGCAAAGAATTCTATTGATGATGCAAACTATTGCTTCTCACAAAGCCGTGAATCGAATGAGCTGCTCAGCCGTGGCAGCTTGCATGGCTCCCTTACTTCTTCGTCCACTCCTAGCTGGGGATTGTGAAATCGACAATGATTTTAGTGTGGGTGGCGACAATTCGGTTCAGCTTTTGCAAGCAGCTGCTGCAGCTAATCATGCTCAAGCAATTGTTATAACTTTGCTGGAGGAGTATGATAACATATTTGGGGAAGGTTCCATATCCGCTGAACTATACAGTGACACAGACGATGATGAAACTGAAACCGAGGGGGAAACTGAAGATGATGAGTACTATGAAGATGATGAAACTGAGTCTGGAGAATACGATGACGATGATGATGTTGAAATTGTCAGTGGGTCATGCAGTGAGAGTGATGAAGATGATGAACAGTTTGACAATAAGGCTGGTTCCAAAGTTCCTGATGGAGGCAATGATAGCAAATCTCCAAAGAAGTTGTCATCAAGCTCACTTAAACATGTGAAGCATCAATATAGTGTAAAAAGCAATGATAATAAACAGAATGTAAGTGAAATTAACTCAGCAGAGCTGGCTAATGAGTCTACTGGAGTAGCAGGTGTTTCTAGAGAAACAAGTTCTGTGCATCAGTCAACTGGCCATGGCCTTCCATGCATGCAAAAATCTTCAACTATATCCAATGGACCTGATTATGGTGCAACGCATCGTCCTAACTGGGGACGTACCGGTGCAAGGAAGAACCTTTCCATGGAATCCATTGACTTCGGTGTTGAGGAGCAGGATGAAGAAATCCAGAGGCTAGAGATTACAAAAGCTGAGTTAGAAAATAAAATTGTAGAAGAGGTTCAAGGGAATGTAGCTCTGCAAGCAAATCTAGAAAAGCAGAAGAAGGCCTTGCTTGAACGTCGTCGAGCTCTCAAACAAGATGTGGCAAGACTACAGGAACAGTTACAGAAAGAGAGAGATTTGGGAGCAGCCCTTGAAGCAGGGCTAAAAATCTCCGAGGGCCATGTACCTAATTTAGCCGATGTTGATGAAAAAACAAGGGCAGAGCTTGAGGAAATGGTTCAGGCAGAGGCGGATTGTACCAACTTGAAAAAGATGGTTGATGATCTAGGTGTGCAGCTTAATCAACAACGTGAACGAAACTATGGCTCTAGCTCTGCGTCTGATGCATCCAATTTGTCGCAACATAGTCATAATACAAAACAGCAGACGAAGGATAAACAGAAGGATAGTGAAGCAGTAGTCCCTTCACATTTTGAATGGTCAAGAAGTAAGGATGGTCATCAAGATGGCACAGAAAATGCAAATGAGAGGAAGGTGGATGGACTACATAAACTCTCCCGACTTGTTGGCGGTATATCCGATTCTTCTGCAGTGGAACCAGTTGTTGCCAAGCATGGCAGGAAAGGGGGTGAGGGGGGTAGTACATCTTCAGCAATAACAAAGTTGACATCGCGACTAAACTTTTTGAAGGAGCGGCGGAGCCAAATAGCTAATGAAATTCAAAGCAGAGATAAAGTTCAATGTTCCCAAAACTTGGAGAAATGTCAATCGCTCCCAGATAATCCGGAAAAAGTTAGAGGATTGGAAGGGGCGGTATCAAGCCAAAAGTCAGAAAAACTCAGAAAGGCTGAGAGTCACTCTGAGAGTAGAGGGAAAAGGTCAGAAGCCCAACACCAGCATATCCTGGACAGAGGAAAATCAGAAAGCCATCTTTCTGTTGACGTGGACAAAGGTCGAGTCGTTGACAGTAGTAGGAGTAAGCCTTTCCCTTCTCCAAGGAAAGACAACAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

922

Amino Acids

100.66

Weight (kDa)

5.31

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 45 - 147 5.7e-12 PH domain
RhoGAP PF00620 205 - 351 1.2e-28 RhoGAP domain
Lzipper-MIP1 PF14389 623 - 701 2.5e-15 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1163
AarI CACCTGC 1 cut(s) 1553
Acc36I ACCTGC 1 cut(s) 1553
AccB1I GGYRCC 1 cut(s) 574
AccB7I CCANNNNNTGG 1 cut(s) 2509
AccBSI CCGCTC 1 cut(s) 2449
AccI GTMKAC 1 cut(s) 1550
AccII CGCG 1 cut(s) 2428
AccIII TCCGGA 1 cut(s) 2539
AciI CCGC 9 cut(s) 62, 69, 679, 1212, 2045, 2325, 2449, 2452, 2567
AclWI GGATC 3 cut(s) 233, 755, 768
AcoI YGGCCR 1 cut(s) 1603
AcsI RAATTY 3 cut(s) 271, 954, 2472
AcuI CTGAAG 3 cut(s) 214, 1287, 2388
AfaI GTAC 8 cut(s) 214, 348, 904, 1280, 1690, 1980, 2053, 2398
AflII CTTAAG 1 cut(s) 386
AflIII ACRYGT 2 cut(s) 766, 1465
AgeI ACCGGT 1 cut(s) 1691
AjiI CACGTC 1 cut(s) 2698
AjnI CCWGG 4 cut(s) 64, 73, 182, 2658
Alw21I GWGCWC 2 cut(s) 613, 1882
AlwI GGATC 3 cut(s) 233, 755, 768
AlwNI CAGNNNCTG 2 cut(s) 824, 1133
Ama87I CYCGRG 1 cut(s) 752
Aor13HI TCCGGA 1 cut(s) 2539
AoxI GGCC 7 cut(s) 70, 142, 588, 1603, 1609, 1857, 1972
ApoI RAATTY 3 cut(s) 271, 954, 2472
ArsI GACNNNNNNTTYG 4 cut(s) 1430, 1462, 2697, 2729
AsiGI ACCGGT 1 cut(s) 1691
Asp700I GAANNNNTTC 2 cut(s) 1809, 2031
AspLEI GCGC 2 cut(s) 25, 464
AspS9I GGNCC 5 cut(s) 71, 143, 529, 1649, 1972
AsuC2I CCSGG 2 cut(s) 17, 926
AsuHPI GGTGA 1 cut(s) 2396
AsuII TTCGAA 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 752
AvaII GGWCC 2 cut(s) 529, 1649
AxyI CCTNAGG 2 cut(s) 27, 635
BalI TGGCCA 1 cut(s) 1605
BamHI GGATCC 1 cut(s) 760
BanI GGYRCC 1 cut(s) 574
BanII GRGCYC 2 cut(s) 613, 1882
BarI GAAGNNNNNNTAC 2 cut(s) 2386, 2418
BauI CACGAG 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 2 cut(s) 613, 1882
BceAI ACGGC 2 cut(s) 977, 1001
BcgI CGANNNNNNTGC 6 cut(s) 77, 111, 990, 1024, 2324, 2358
BciT130I CCWGG 4 cut(s) 66, 75, 184, 2660
BcnI CCSGG 2 cut(s) 17, 926
BfaI CTAG 9 cut(s) 243, 644, 848, 1058, 1572, 1766, 1841, 2081, 2124
BfmI CTRYAG 4 cut(s) 687, 1134, 1904, 2343
BfoI RGCGCY 2 cut(s) 26, 465
BfrI CTTAAG 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 1553
BglI GCCNNNNNGGC 1 cut(s) 78
BglII AGATCT 1 cut(s) 406
BlpI GCTNAGC 1 cut(s) 1012
BmcAI AGTACT 1 cut(s) 1280
Bme1390I CCNGG 6 cut(s) 17, 66, 75, 184, 926, 2660
Bme18I GGWCC 2 cut(s) 529, 1649
BmeT110I CYCGRG 1 cut(s) 752
BmgBI CACGTC 1 cut(s) 2698
BmgT120I GGNCC 5 cut(s) 71, 143, 529, 1649, 1972
BmiI GGNNCC 9 cut(s) 576, 641, 762, 930, 1035, 1204, 1399, 2352, 2456
BmrFI CCNGG 6 cut(s) 17, 66, 75, 184, 926, 2660
BmrI ACTGGG 1 cut(s) 1690
BmsI GCATC 7 cut(s) 836, 957, 1483, 1600, 1678, 2128, 2150
BmuI ACTGGG 1 cut(s) 1690
BoxI GACNNNNGTC 1 cut(s) 2706
BpiI GAAGAC 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 2597, 2629
BpmI CTGGAG 4 cut(s) 801, 1193, 1326, 1575
Bpu1102I GCTNAGC 1 cut(s) 1012
Bpu14I TTCGAA 1 cut(s) 401
BpuEI CTTGAG 2 cut(s) 1134, 2042
BpuMI CCSGG 2 cut(s) 17, 926
BsaBI GATNNNNATC 2 cut(s) 178, 2253
BsaWI WCCGGW 2 cut(s) 1691, 2539
BsaXI ACNNNNNCTCC 2 cut(s) 648, 678
Bse118I RCCGGY 1 cut(s) 1691
Bse1I ACTGG 6 cut(s) 435, 818, 1558, 1606, 1685, 2354
Bse21I CCTNAGG 2 cut(s) 27, 635
Bse3DI GCAATG 3 cut(s) 771, 1426, 1501
Bse8I GATNNNNATC 2 cut(s) 178, 2253
BseAI TCCGGA 1 cut(s) 2539
BseBI CCWGG 4 cut(s) 66, 75, 184, 2660
BseGI GGATG 7 cut(s) 40, 179, 179, 1753, 2141, 2254, 2299
BseJI GATNNNNATC 2 cut(s) 178, 2253
BseMI GCAATG 3 cut(s) 771, 1426, 1501
BseMII CTCAG 8 cut(s) 41, 1026, 1290, 1542, 1773, 2595, 2607, 2608
BseNI ACTGG 6 cut(s) 435, 818, 1558, 1606, 1685, 2354
BseRI GAGGAG 4 cut(s) 39, 770, 1190, 1754
BseYI CCCAGC 1 cut(s) 1061
BsgI GTGCAG 1 cut(s) 2108
Bsh1236I CGCG 1 cut(s) 2428
BshFI GGCC 7 cut(s) 72, 144, 590, 1605, 1611, 1859, 1974
BshNI GGYRCC 1 cut(s) 574
BshTI ACCGGT 1 cut(s) 1691
BsiHKAI GWGCWC 2 cut(s) 613, 1882
BsiHKCI CYCGRG 1 cut(s) 752
BsiSI CCGG 4 cut(s) 17, 926, 1692, 2540
BsiWI CGTACG 1 cut(s) 902
BslFI GGGAC 3 cut(s) 806, 1698, 2201
BsmFI GGGAC 3 cut(s) 806, 1698, 2201
BsnI GGCC 7 cut(s) 72, 144, 590, 1605, 1611, 1859, 1974
BsoBI CYCGRG 1 cut(s) 752
Bsp119I TTCGAA 1 cut(s) 401
Bsp1286I GDGCHC 2 cut(s) 613, 1882
Bsp13I TCCGGA 1 cut(s) 2539
Bsp143I GATC 6 cut(s) 88, 238, 406, 760, 874, 2077
Bsp1720I GCTNAGC 1 cut(s) 1012
Bsp19I CCATGG 2 cut(s) 1606, 1713
Bsp68I TCGCGA 1 cut(s) 2428
BspACI CCGC 9 cut(s) 62, 69, 679, 1212, 2045, 2325, 2449, 2452, 2567
BspANI GGCC 7 cut(s) 72, 144, 590, 1605, 1611, 1859, 1974
BspCNI CTCAG 8 cut(s) 40, 1025, 1291, 1541, 1774, 2596, 2607, 2608
BspEI TCCGGA 1 cut(s) 2539
BspFNI CGCG 1 cut(s) 2428
BspLI GGNNCC 9 cut(s) 576, 641, 762, 930, 1035, 1204, 1399, 2352, 2456
BspMAI CTGCAG 3 cut(s) 691, 1138, 2347
BspMI ACCTGC 1 cut(s) 1553
BspPI GGATC 3 cut(s) 233, 755, 768
BspT104I TTCGAA 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 574
BspTI CTTAAG 1 cut(s) 386
BsrBI CCGCTC 1 cut(s) 2449
BsrDI GCAATG 3 cut(s) 771, 1426, 1501
BsrFI RCCGGY 1 cut(s) 1691
BsrI ACTGG 6 cut(s) 435, 818, 1558, 1606, 1685, 2354
BssAI RCCGGY 1 cut(s) 1691
BssMI GATC 6 cut(s) 88, 238, 406, 760, 874, 2077
BssSI CACGAG 1 cut(s) 114
BssT1I CCWWGG 5 cut(s) 280, 591, 1606, 1713, 2750
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 4 cut(s) 66, 75, 184, 2660
Bst4CI ACNGT 7 cut(s) 130, 346, 938, 1226, 1380, 1914, 2723
Bst6I CTCTTC 1 cut(s) 1800
BstAFI CTTAAG 1 cut(s) 386
BstAPI GCANNNNNTGC 1 cut(s) 1133
BstBI TTCGAA 1 cut(s) 401
BstC8I GCNNGC 8 cut(s) 375, 588, 684, 1027, 1125, 1539, 1622, 1833
BstDEI CTNAG 9 cut(s) 27, 635, 1012, 1299, 1528, 1782, 2594, 2604, 2616
BstDSI CCRYGG 3 cut(s) 1017, 1606, 1713
BstENI CCTNNNNNAGG 1 cut(s) 1413
BstF5I GGATG 7 cut(s) 40, 179, 179, 1753, 2141, 2254, 2299
BstFNI CGCG 1 cut(s) 2428
BstH2I RGCGCY 2 cut(s) 26, 465
BstHHI GCGC 2 cut(s) 25, 464
BstKTI GATC 6 cut(s) 91, 241, 409, 763, 877, 2080
BstMBI GATC 6 cut(s) 88, 238, 406, 760, 874, 2077
BstNI CCWGG 4 cut(s) 66, 75, 184, 2660
BstNSI RCATGY 3 cut(s) 770, 1469, 1624
BstPAI GACNNNNGTC 1 cut(s) 2706
BstSCI CCNGG 6 cut(s) 15, 64, 73, 182, 924, 2658
BstSFI CTRYAG 4 cut(s) 687, 1134, 1904, 2343
BstUI CGCG 1 cut(s) 2428
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 2 cut(s) 406, 760
BstYI RGATCY 2 cut(s) 406, 760
Bsu36I CCTNAGG 2 cut(s) 27, 635
BsuRI GGCC 7 cut(s) 72, 144, 590, 1605, 1611, 1859, 1974
BtgI CCRYGG 3 cut(s) 1017, 1606, 1713
BtgZI GCGATG 1 cut(s) 2409
BtrI CACGTC 1 cut(s) 2698
BtsCI GGATG 7 cut(s) 40, 179, 179, 1753, 2141, 2254, 2299
BtsI GCAGTG 4 cut(s) 316, 842, 1360, 2352
BtsIMutI CAGTG 7 cut(s) 316, 428, 842, 1231, 1348, 1360, 2352
BtuMI TCGCGA 1 cut(s) 2428
BveI ACCTGC 1 cut(s) 1553
Cac8I GCNNGC 8 cut(s) 375, 588, 684, 1027, 1125, 1539, 1622, 1833
CaiI CAGNNNCTG 2 cut(s) 824, 1133
CfoI GCGC 2 cut(s) 25, 464
Cfr10I RCCGGY 1 cut(s) 1691
Cfr13I GGNCC 5 cut(s) 71, 143, 529, 1649, 1972
CseI GACGC 1 cut(s) 2121
Csp6I GTAC 8 cut(s) 213, 347, 903, 1279, 1689, 1979, 2052, 2397
CspAI ACCGGT 1 cut(s) 1691
CviQI GTAC 8 cut(s) 213, 347, 903, 1279, 1689, 1979, 2052, 2397
DdeI CTNAG 9 cut(s) 27, 635, 1012, 1299, 1528, 1782, 2594, 2604, 2616
DpnI GATC 6 cut(s) 90, 240, 408, 762, 876, 2079
DpnII GATC 6 cut(s) 88, 238, 406, 760, 874, 2077
EaeI YGGCCR 1 cut(s) 1603
Eam1104I CTCTTC 1 cut(s) 1800
EarI CTCTTC 1 cut(s) 1800
EciI GGCGGA 2 cut(s) 2060, 2467
Ecl136II GAGCTC 2 cut(s) 611, 1880
Eco130I CCWWGG 5 cut(s) 280, 591, 1606, 1713, 2750
Eco147I AGGCCT 1 cut(s) 1859
Eco24I GRGCYC 2 cut(s) 613, 1882
Eco47I GGWCC 2 cut(s) 529, 1649
Eco53kI GAGCTC 2 cut(s) 611, 1880
Eco57I CTGAAG 3 cut(s) 214, 1287, 2388
Eco81I CCTNAGG 2 cut(s) 27, 635
Eco88I CYCGRG 1 cut(s) 752
EcoICRI GAGCTC 2 cut(s) 611, 1880
EcoNI CCTNNNNNAGG 1 cut(s) 1413
EcoRI GAATTC 1 cut(s) 954
EcoRII CCWGG 4 cut(s) 64, 73, 182, 2658
EcoT14I CCWWGG 5 cut(s) 280, 591, 1606, 1713, 2750
EcoT22I ATGCAT 2 cut(s) 1622, 2143
EcoT38I GRGCYC 2 cut(s) 613, 1882
ErhI CCWWGG 5 cut(s) 280, 591, 1606, 1713, 2750
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 1844, 1876
FaqI GGGAC 3 cut(s) 806, 1698, 2201
FauI CCCGC 1 cut(s) 69
FblI GTMKAC 1 cut(s) 1550
FokI GGATG 7 cut(s) 47, 166, 186, 1760, 2128, 2261, 2306
FriOI GRGCYC 2 cut(s) 613, 1882
FspBI CTAG 9 cut(s) 243, 644, 848, 1058, 1572, 1766, 1841, 2081, 2124
GlaI GCGC 2 cut(s) 24, 463
GsaI CCCAGC 1 cut(s) 1065
GsuI CTGGAG 4 cut(s) 801, 1193, 1326, 1575
HaeII RGCGCY 2 cut(s) 26, 465
HaeIII GGCC 7 cut(s) 72, 144, 590, 1605, 1611, 1859, 1974
HapII CCGG 4 cut(s) 17, 926, 1692, 2540
HgaI GACGC 1 cut(s) 2121
HhaI GCGC 2 cut(s) 25, 464
Hin6I GCGC 2 cut(s) 23, 462
HinP1I GCGC 2 cut(s) 23, 462
HincII GTYRAC 4 cut(s) 1599, 2421, 2695, 2719
HindII GTYRAC 4 cut(s) 1599, 2421, 2695, 2719
HpaII CCGG 4 cut(s) 17, 926, 1692, 2540
HphI GGTGA 1 cut(s) 2396
Hpy166II GTNNAC 8 cut(s) 1052, 1551, 1599, 2108, 2421, 2695, 2701, 2719
Hpy8I GTNNAC 8 cut(s) 1052, 1551, 1599, 2108, 2421, 2695, 2701, 2719
Hpy99I CGWCG 3 cut(s) 942, 1875, 1878
HpyCH4III ACNGT 7 cut(s) 130, 346, 938, 1226, 1380, 1914, 2723
HpyCH4IV ACGT 4 cut(s) 1687, 1870, 2104, 2697
HpyF3I CTNAG 9 cut(s) 27, 635, 1012, 1299, 1528, 1782, 2594, 2604, 2616
HpySE526I ACGT 4 cut(s) 1687, 1870, 2104, 2697
HspAI GCGC 2 cut(s) 23, 462
Kpn2I TCCGGA 1 cut(s) 2539
Kzo9I GATC 6 cut(s) 88, 238, 406, 760, 874, 2077
LmnI GCTCC 9 cut(s) 457, 608, 616, 1039, 1741, 1937, 2446, 2454, 2532
LweI GCATC 7 cut(s) 836, 957, 1483, 1600, 1678, 2128, 2150
MaeI CTAG 9 cut(s) 243, 644, 848, 1058, 1572, 1766, 1841, 2081, 2124
MaeII ACGT 4 cut(s) 1687, 1870, 2104, 2697
MaeIII GTNAC 4 cut(s) 836, 1226, 1914, 2609
MalI GATC 6 cut(s) 90, 240, 408, 762, 876, 2079
MbiI CCGCTC 1 cut(s) 2449
MboI GATC 6 cut(s) 88, 238, 406, 760, 874, 2077
MfeI CAATTG 1 cut(s) 1155
MflI RGATCY 2 cut(s) 406, 760
MhlI GDGCHC 2 cut(s) 613, 1882
MlsI TGGCCA 1 cut(s) 1605
MluNI TGGCCA 1 cut(s) 1605
MlyI GAGTC 6 cut(s) 503, 666, 1310, 1556, 2617, 2721
Mox20I TGGCCA 1 cut(s) 1605
Mph1103I ATGCAT 2 cut(s) 1622, 2143
MroI TCCGGA 1 cut(s) 2539
MroXI GAANNNNTTC 2 cut(s) 1809, 2031
MscI TGGCCA 1 cut(s) 1605
MseI TTAA 5 cut(s) 206, 387, 1461, 1524, 2094
MslI CAYNNNNRTG 1 cut(s) 2259
Msp20I TGGCCA 1 cut(s) 1605
MspA1I CMGCKG 2 cut(s) 1130, 1214
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 4 cut(s) 17, 926, 1692, 2540
MspR9I CCNGG 6 cut(s) 17, 66, 75, 184, 926, 2660
MunI CAATTG 1 cut(s) 1155
MvaI CCWGG 4 cut(s) 66, 75, 184, 2660
MvnI CGCG 1 cut(s) 2428
NciI CCSGG 2 cut(s) 17, 926
NcoI CCATGG 2 cut(s) 1606, 1713
NdeII GATC 6 cut(s) 88, 238, 406, 760, 874, 2077
NlaIV GGNNCC 9 cut(s) 576, 641, 762, 930, 1035, 1204, 1399, 2352, 2456
NmuCI GTSAC 2 cut(s) 1226, 2609
NruI TCGCGA 1 cut(s) 2428
NsiI ATGCAT 2 cut(s) 1622, 2143
NspI RCATGY 3 cut(s) 770, 1469, 1624
NspV TTCGAA 1 cut(s) 401
PaeI GCATGC 1 cut(s) 1624
PaqCI CACCTGC 1 cut(s) 1553
PceI AGGCCT 1 cut(s) 1859
PciI ACATGT 2 cut(s) 766, 1465
PcsI WCGNNNNNNNCGW 1 cut(s) 370
PdmI GAANNNNTTC 2 cut(s) 1809, 2031
PfeI GAWTC 8 cut(s) 101, 672, 707, 851, 888, 997, 1718, 2336
Pfl23II CGTACG 1 cut(s) 902
PflMI CCANNNNNTGG 1 cut(s) 2509
PfoI TCCNGGA 3 cut(s) 182, 924, 2658
PinAI ACCGGT 1 cut(s) 1691
PleI GAGTC 6 cut(s) 503, 665, 1309, 1555, 2616, 2720
PpsI GAGTC 6 cut(s) 503, 665, 1309, 1555, 2616, 2720
PscI ACATGT 2 cut(s) 766, 1465
PshAI GACNNNNGTC 1 cut(s) 2706
PsiI TTATAA 1 cut(s) 1163
Psp124BI GAGCTC 2 cut(s) 613, 1882
Psp6I CCWGG 4 cut(s) 64, 73, 182, 2658
PspFI CCCAGC 1 cut(s) 1061
PspGI CCWGG 4 cut(s) 64, 73, 182, 2658
PspLI CGTACG 1 cut(s) 902
PspN4I GGNNCC 9 cut(s) 576, 641, 762, 930, 1035, 1204, 1399, 2352, 2456
PspPI GGNCC 5 cut(s) 71, 143, 529, 1649, 1972
PsrI GAACNNNNNNTAC 2 cut(s) 1794, 1826
PstI CTGCAG 3 cut(s) 691, 1138, 2347
PstNI CAGNNNCTG 2 cut(s) 824, 1133
PsuI RGATCY 2 cut(s) 406, 760
PvuII CAGCTG 1 cut(s) 1130
RruI TCGCGA 1 cut(s) 2428
RsaI GTAC 8 cut(s) 214, 348, 904, 1280, 1690, 1980, 2053, 2398
RsaNI GTAC 8 cut(s) 213, 347, 903, 1279, 1689, 1979, 2052, 2397
RseI CAYNNNNRTG 1 cut(s) 2259
SacI GAGCTC 2 cut(s) 613, 1882
SaqAI TTAA 5 cut(s) 206, 387, 1461, 1524, 2094
Sau3AI GATC 6 cut(s) 88, 238, 406, 760, 874, 2077
Sau96I GGNCC 5 cut(s) 71, 143, 529, 1649, 1972
ScaI AGTACT 1 cut(s) 1280
SchI GAGTC 6 cut(s) 503, 666, 1310, 1556, 2617, 2721
ScrFI CCNGG 6 cut(s) 17, 66, 75, 184, 926, 2660
SduI GDGCHC 2 cut(s) 613, 1882
SfaNI GCATC 7 cut(s) 836, 957, 1483, 1600, 1678, 2128, 2150
SfcI CTRYAG 4 cut(s) 687, 1134, 1904, 2343
SfuI TTCGAA 1 cut(s) 401
SinI GGWCC 2 cut(s) 529, 1649
SmiMI CAYNNNNRTG 1 cut(s) 2259
SmlI CTYRAG 3 cut(s) 386, 1149, 2021
SmoI CTYRAG 3 cut(s) 386, 1149, 2021
SphI GCATGC 1 cut(s) 1624
SseBI AGGCCT 1 cut(s) 1859
SsiI CCGC 9 cut(s) 62, 69, 679, 1212, 2045, 2325, 2449, 2452, 2567
SspMI CTAG 9 cut(s) 243, 644, 848, 1058, 1572, 1766, 1841, 2081, 2124
SstI GAGCTC 2 cut(s) 613, 1882
StuI AGGCCT 1 cut(s) 1859
StyD4I CCNGG 6 cut(s) 15, 64, 73, 182, 924, 2658
StyI CCWWGG 5 cut(s) 280, 591, 1606, 1713, 2750
TaaI ACNGT 7 cut(s) 130, 346, 938, 1226, 1380, 1914, 2723
TaiI ACGT 4 cut(s) 1690, 1873, 2107, 2700
TaqI TCGA 6 cut(s) 401, 712, 1000, 1077, 1876, 2710
TatI WGTACW 3 cut(s) 346, 1278, 2396
TauI GCSGC 3 cut(s) 72, 682, 2452
TfiI GAWTC 8 cut(s) 101, 672, 707, 851, 888, 997, 1718, 2336
Tru1I TTAA 5 cut(s) 206, 387, 1461, 1524, 2094
Tru9I TTAA 5 cut(s) 206, 387, 1461, 1524, 2094
TscAI CASTG 7 cut(s) 316, 435, 849, 1231, 1348, 1360, 2352
TseFI GTSAC 2 cut(s) 1226, 2609
Tsp45I GTSAC 2 cut(s) 1226, 2609
TspDTI ATGAA 9 cut(s) 429, 1257, 1299, 1308, 1380, 1389, 1764, 2016, 2484
TspRI CASTG 7 cut(s) 316, 435, 849, 1231, 1348, 1360, 2352
Van91I CCANNNNNTGG 1 cut(s) 2509
Vha464I CTTAAG 1 cut(s) 386
VpaK11BI GGWCC 2 cut(s) 529, 1649
XagI CCTNNNNNAGG 1 cut(s) 1413
XapI RAATTY 3 cut(s) 271, 954, 2472
XbaI TCTAGA 2 cut(s) 1571, 1840
XceI RCATGY 3 cut(s) 770, 1469, 1624
XcmI CCANNNNNNNNNTGG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 1550
XmnI GAANNNNTTC 2 cut(s) 1809, 2031
XspI CTAG 9 cut(s) 243, 644, 848, 1058, 1572, 1766, 1841, 2081, 2124
ZrmI AGTACT 1 cut(s) 1280
Zsp2I ATGCAT 2 cut(s) 1622, 2143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.