Rroxscaffold_3G00224500

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0007329
Physical Location & Seq
Forward (+)
7703410 .. 7717260
13851 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00224500.1

Sequence Viewer

Length: 2538 bp
ATGACTAACATAATCCCGGAAGCGCCTCAGGGGGATGGTCATGCTTCTCCTCCCCAACCCCCGCCTGGCGGCCCTGGTGGCGACCGCGATCATCATGCTGAATCTCATGGTTCTCGTGGTGGCAATACGGTTTTCAAGAGTGGCCCACTATTTATATCTTCCAAAGGAATTGGATGGACATCTTGGAAAAAGAGATGGTTTATTTTAACTCGTACTTCGCTGGTTTTCTTCAGAAGTGATCCTAGTGCTGTTCAGAAGGGGAGTGAAGTGAATTTGACCCTTGGTGGTATTGACCTCAACAATTCAGGCAGTGTGGTTGTCAAAGAAGACAAGAAACTCTTGACTGTACTCTTTCCAGATGGTCGTGATGGGCGAGCCTTCACACTTAAGGCTGAAACTTCGAAAGATCTACATGAATGGAAGGAAGCACTGGATAACGCTTTGGCACAAGCTCCAAGTGGCGCTCATGGTGTGGGGCAAAATGGTATCTTGGGGAATGATCAGACAGACTCCAAAAATGGTTCTTTGGACCAGCCAAAGGAGAAACCACCTGTGAAATCTACAATTATTGGGGTGCCTATTTTGCTGGCCTTGGAAGATGTTGATGGAGCTCCATGTTTCTTGGAAAAAGCCCTTAGGTTCCTAGAAGAACATGGAGTCAAGGTGGAAGGAATCTTGCGACAAGCTGCAGATGTTGATGATGTTGAATCTCGAATACGGGAATATGAGAAGGGGAAAGTTGATTTTTCTCCCGAGGAGGATCCACATGTCATTGCTGATTGTGTCAAGTATGTCCTCCGAGAGTTGCCATCTTCTCCAGTCCCTGCATCTTGCTGTAACGCACTGCTAGATTCCTTTCGCAAGTCTGCCTCTATGGCTGAAAATAATTTGGAAATGGAGATCATTGAAACTTCTGTTCTAATTCTATTGATGATGCAAACTATTGCTTCTCACAAAGCCGTGAATCGAATGAGCTGCTCAGCCGTGGCAGCTTGCATGGCTCCCTTACTTCTTCGTCCACTCCTAGCTGGGGATTGTGAAATCGACAATGATTTTAGTGTGGGTGGCGACAATTCAGTTCAGCTTTTGCAAGCAGCAGCTGCAGCTAATCATGCTCAAGCAATTGTTATAACTTTGCTGGAGGAGTATGATAACATATTTGGGGCTGGTTCCAAGGTTCCTGATGGAGGTAATGATAGCAAATCTCCAAAGAAGTTGTCATCAAGCTCACTTAAACATGTGAAGCATCAATATAGTGTAAAAAGCAATGATAATAAACAGAATGCAAGTGAAATTAACTCAGCAGAGCAGGCTAATGAGTCTACTGGAGTAGCAGGTGTTTCTAGAGAAACAAGTTCTGTGCATCAGTCAACTGGCCATGGCCTTCCATGCATGCAAAAATCTTCAACTATATCCAATGGACCTGATTATGGTGCAACGCATCGTCCTAACTGGGGACGTACCGGTGCAAGGAAGAACCTTTCCATGGAATCCATTGACTTTGGTGTTGAGGAGGAGGATGAAGAAATCCAGAGGCTAGAGATTACAAAAGCTGAGTTAGAAAATAAAATTGTAGAAGAGGTTCAAGGGAATGTAGCTCTGCAAGCAAATCTAGAAAAGCAGAAGAAGGCCTTGCTTGAACGTCGTCGAGCTCTCAAACAAGATGTGGCAAGACTACAGGAACAGTTACAGAAGGAGAGAGATTTGGGAGCAGCCCTTGAAGCAGGGCTAAAAATCTCCGAGGGCCATGTACCTAATTTAGCCGATGTTGATGAAAAAACAAGGGCAGAGCTTGAGGAAATGGTTCAGGCAGAGGCGGATTGTACCAACTTGAAAAAGAAGGTTGATGATCTAGGTGTGCAGCTTAATCAACAACGTGAACGAAACTATGGCTCTAGCTCTGCGCAGACGAAGGATAAACAGAAGGATAGTGAAGCAGTAGTCCCTTCACATTTTGAATGGTCAAGAAGTAAGGAGGGTCATCAAGATGGCACAGAAAATGCAAATGAGAGGAAGGTGGATGGACTACATAAACTCTCCCGACTTGTTGGCGGTATATCCGATTCTTCTGCAGTGGAACCACTTGTTGCCAAGCATGGCAAGAAAGGGGTTGAGCTGCGTGATGGTGGGTCCGGGGTTGATCTTAGTTTGGATCGGAGCATTAGTGATGTGGATTCTCCGGGGAGGATAGATGCGAGGATGGGGGGTGATGGTGGATCGGTCTTGCTTGTCGGGTCCGGGTTCTTGTGGATCGGATCGATTATGCGGTCTGTCCATGATTGTGATTATGCGGACGGTGGTCAAGGTTCCCGTCGGTGTGGTGGTGTTCATTATGGTGGCGGTGGTGGTCTAGTTGGGGGCGTTAGTTTCTATGGTGACGGCGGTGGCTTCTACGAGGAGGGCGACGGCGGAATCATACGGGAGGGTAGAGTTTCTTCTCAATGGGCTTGTTGGGCTCTAGTTAGGGGTTCGGCTCTAGGGTTATGGTCAATTTTGGCTGGGTTCTCGGGTCCTTGGGCGTATGTTTTCGTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0000904 GO:0001891 GO:0002009 GO:0002011 GO:0003006 GO:0003674 GO:0005096 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005938 GO:0006810 GO:0006897 GO:0006909 GO:0006911 GO:0006996 GO:0007010 GO:0007015 GO:0007154 GO:0007155 GO:0008047 GO:0008150 GO:0009653 GO:0009826 GO:0009846 GO:0009856 GO:0009860 GO:0009865 GO:0009875 GO:0009888 GO:0009932 GO:0009966 GO:0009968 GO:0009987 GO:0010324 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0016324 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022610 GO:0023051 GO:0023057 GO:0030029 GO:0030036 GO:0030133 GO:0030154 GO:0030234 GO:0030695 GO:0031267 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032989 GO:0035023 GO:0035024 GO:0040007 GO:0042995 GO:0043085 GO:0043087 GO:0043226 GO:0043227 GO:0043229 GO:0043547 GO:0044093 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0044703 GO:0044706 GO:0045177 GO:0046578 GO:0046580 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048588 GO:0048589 GO:0048729 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050790 GO:0050794 GO:0051020 GO:0051056 GO:0051058 GO:0051179 GO:0051234 GO:0051336 GO:0051345 GO:0051704 GO:0060429 GO:0060560 GO:0060589 GO:0061024 GO:0065007 GO:0065009 GO:0070382 GO:0071840 GO:0071944 GO:0090406 GO:0090630 GO:0097435 GO:0097708 GO:0098590 GO:0098609 GO:0098657 GO:0098740 GO:0098772 GO:0099024 GO:0099503 GO:0099568 GO:0120025 GO:1902531 GO:1902532
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lzipper-MIP1 PH RhoGAP

Protein Analysis

845

Amino Acids

90.63

Weight (kDa)

5.41

Isoelectric Point (pI)

44.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 45 - 147 5.1e-12 PH domain
RhoGAP PF00620 205 - 340 6.7e-20 RhoGAP domain
Lzipper-MIP1 PF14389 547 - 625 4.6e-16 Leucine-zipper of ternary complex factor MIP1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1130
AarI CACCTGC 1 cut(s) 1325
Acc16I TGCGCA 1 cut(s) 1905
Acc36I ACCTGC 1 cut(s) 1325
AccB1I GGYRCC 1 cut(s) 574
AccI GTMKAC 1 cut(s) 1322
AccII CGCG 1 cut(s) 87
AclWI GGATC 7 cut(s) 233, 755, 768, 2160, 2224, 2258, 2263
AcoI YGGCCR 1 cut(s) 1375
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 214
AfaI GTAC 5 cut(s) 214, 348, 1462, 1752, 1825
AfiI CCNNNNNNNGG 9 cut(s) 65, 68, 538, 1030, 1187, 1430, 1454, 1470, 1486
AflII CTTAAG 1 cut(s) 386
AflIII ACRYGT 2 cut(s) 766, 1237
AgeI ACCGGT 1 cut(s) 1463
AgsI TTSAA 9 cut(s) 136, 707, 908, 1407, 1586, 1640, 1721, 1834, 1958
AjnI CCWGG 2 cut(s) 64, 73
Alw21I GWGCWC 2 cut(s) 613, 1654
AlwI GGATC 7 cut(s) 233, 755, 768, 2160, 2224, 2258, 2263
AlwNI CAGNNNCTG 2 cut(s) 824, 1100
Ama87I CYCGRG 2 cut(s) 752, 2506
AoxI GGCC 7 cut(s) 70, 142, 588, 1375, 1381, 1629, 1744
ApoI RAATTY 1 cut(s) 271
ArsI GACNNNNNNTTYG 2 cut(s) 1202, 1234
AsiGI ACCGGT 1 cut(s) 1463
Asp700I GAANNNNTTC 2 cut(s) 1581, 1803
AspLEI GCGC 3 cut(s) 25, 464, 1906
AspS9I GGNCC 8 cut(s) 71, 143, 529, 1421, 1744, 2130, 2235, 2510
AsuC2I CCSGG 4 cut(s) 17, 2134, 2181, 2239
AsuHPI GGTGA 2 cut(s) 2219, 2387
AsuII TTCGAA 1 cut(s) 401
AvaI CYCGRG 2 cut(s) 752, 2506
AvaII GGWCC 5 cut(s) 529, 1421, 2130, 2235, 2510
AxyI CCTNAGG 2 cut(s) 27, 635
BalI TGGCCA 1 cut(s) 1377
BamHI GGATCC 1 cut(s) 760
BanI GGYRCC 1 cut(s) 574
BanII GRGCYC 3 cut(s) 613, 1654, 2458
BarI GAAGNNNNNNTAC 2 cut(s) 2419, 2451
BauI CACGAG 1 cut(s) 114
BbsI GAAGAC 1 cut(s) 333
Bbv12I GWGCWC 2 cut(s) 613, 1654
BceAI ACGGC 4 cut(s) 944, 968, 2395, 2422
BcgI CGANNNNNNTGC 6 cut(s) 77, 111, 957, 991, 2051, 2085
BciT130I CCWGG 2 cut(s) 66, 75
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 4 cut(s) 17, 2134, 2181, 2239
BfmI CTRYAG 4 cut(s) 687, 1101, 1676, 2070
BfoI RGCGCY 2 cut(s) 26, 465
BfrI CTTAAG 1 cut(s) 386
BfuAI ACCTGC 1 cut(s) 1325
BglI GCCNNNNNGGC 2 cut(s) 78, 875
BglII AGATCT 1 cut(s) 406
BlpI GCTNAGC 1 cut(s) 979
Bme1390I CCNGG 6 cut(s) 17, 66, 75, 2134, 2181, 2239
Bme18I GGWCC 5 cut(s) 529, 1421, 2130, 2235, 2510
BmeT110I CYCGRG 2 cut(s) 752, 2506
BmgT120I GGNCC 8 cut(s) 71, 143, 529, 1421, 1744, 2130, 2235, 2510
BmrFI CCNGG 6 cut(s) 17, 66, 75, 2134, 2181, 2239
BmrI ACTGGG 1 cut(s) 1462
BmsI GCATC 6 cut(s) 836, 924, 1255, 1372, 1450, 2182
BmuI ACTGGG 1 cut(s) 1462
BoxI GACNNNNGTC 1 cut(s) 2298
BpiI GAAGAC 1 cut(s) 333
BpmI CTGGAG 3 cut(s) 801, 1160, 1347
Bpu1102I GCTNAGC 1 cut(s) 979
Bpu14I TTCGAA 1 cut(s) 401
BpuEI CTTGAG 2 cut(s) 1101, 1814
BpuMI CCSGG 4 cut(s) 17, 2134, 2181, 2239
Bsa29I ATCGAT 1 cut(s) 2258
BsaBI GATNNNNATC 1 cut(s) 178
BsaWI WCCGGW 1 cut(s) 1463
BsaXI ACNNNNNCTCC 4 cut(s) 648, 678, 2149, 2179
Bsc4I CCNNNNNNNGG 9 cut(s) 65, 68, 538, 1030, 1187, 1430, 1454, 1470, 1486
Bse118I RCCGGY 1 cut(s) 1463
Bse1I ACTGG 5 cut(s) 435, 818, 1330, 1378, 1457
Bse21I CCTNAGG 2 cut(s) 27, 635
Bse3DI GCAATG 2 cut(s) 771, 1273
Bse8I GATNNNNATC 1 cut(s) 178
BseBI CCWGG 2 cut(s) 66, 75
BseCI ATCGAT 1 cut(s) 2258
BseGI GGATG 5 cut(s) 40, 179, 1525, 2026, 2205
BseJI GATNNNNATC 1 cut(s) 178
BseLI CCNNNNNNNGG 9 cut(s) 65, 68, 538, 1030, 1187, 1430, 1454, 1470, 1486
BseMI GCAATG 2 cut(s) 771, 1273
BseMII CTCAG 4 cut(s) 41, 993, 1314, 1545
BseNI ACTGG 5 cut(s) 435, 818, 1330, 1378, 1457
BseRI GAGGAG 6 cut(s) 39, 770, 1157, 1526, 1529, 2411
BseYI CCCAGC 2 cut(s) 1028, 2498
BsgI GTGCAG 1 cut(s) 1880
Bsh1236I CGCG 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 85
BshFI GGCC 7 cut(s) 72, 144, 590, 1377, 1383, 1631, 1746
BshNI GGYRCC 1 cut(s) 574
BshTI ACCGGT 1 cut(s) 1463
BshVI ATCGAT 1 cut(s) 2258
BsiEI CGRYCG 1 cut(s) 85
BsiHKAI GWGCWC 2 cut(s) 613, 1654
BsiHKCI CYCGRG 2 cut(s) 752, 2506
BsiSI CCGG 5 cut(s) 17, 1464, 2133, 2180, 2238
BslFI GGGAC 3 cut(s) 806, 1470, 1928
BslI CCNNNNNNNGG 9 cut(s) 65, 68, 538, 1030, 1187, 1430, 1454, 1470, 1486
BsmFI GGGAC 3 cut(s) 806, 1470, 1928
BsmI GAATGC 1 cut(s) 1288
BsnI GGCC 7 cut(s) 72, 144, 590, 1377, 1383, 1631, 1746
BsoBI CYCGRG 2 cut(s) 752, 2506
Bsp119I TTCGAA 1 cut(s) 401
Bsp1286I GDGCHC 3 cut(s) 613, 1654, 2458
Bsp1720I GCTNAGC 1 cut(s) 979
Bsp19I CCATGG 2 cut(s) 1378, 1485
BspANI GGCC 7 cut(s) 72, 144, 590, 1377, 1383, 1631, 1746
BspCNI CTCAG 4 cut(s) 40, 992, 1313, 1546
BspDI ATCGAT 1 cut(s) 2258
BspFNI CGCG 1 cut(s) 87
BspMAI CTGCAG 3 cut(s) 691, 1105, 2074
BspMI ACCTGC 1 cut(s) 1325
BspPI GGATC 7 cut(s) 233, 755, 768, 2160, 2224, 2258, 2263
BspT104I TTCGAA 1 cut(s) 401
BspT107I GGYRCC 1 cut(s) 574
BspTI CTTAAG 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 771, 1273
BsrFI RCCGGY 1 cut(s) 1463
BsrI ACTGG 5 cut(s) 435, 818, 1330, 1378, 1457
BssAI RCCGGY 1 cut(s) 1463
BssSI CACGAG 1 cut(s) 114
BssT1I CCWWGG 6 cut(s) 280, 591, 1173, 1378, 1485, 2513
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 66, 75
Bst4CI ACNGT 4 cut(s) 130, 346, 1686, 2297
Bst6I CTCTTC 1 cut(s) 1572
BstAFI CTTAAG 1 cut(s) 386
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstBI TTCGAA 1 cut(s) 401
BstC8I GCNNGC 7 cut(s) 375, 588, 994, 1092, 1311, 1394, 1605
BstDEI CTNAG 6 cut(s) 27, 635, 979, 1300, 1554, 2144
BstDSI CCRYGG 3 cut(s) 984, 1378, 1485
BstENI CCTNNNNNAGG 1 cut(s) 1185
BstF5I GGATG 5 cut(s) 40, 179, 1525, 2026, 2205
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 2 cut(s) 26, 465
BstHHI GCGC 3 cut(s) 25, 464, 1906
BstMCI CGRYCG 1 cut(s) 85
BstNI CCWGG 2 cut(s) 66, 75
BstNSI RCATGY 3 cut(s) 770, 1241, 1396
BstPAI GACNNNNGTC 1 cut(s) 2298
BstSCI CCNGG 6 cut(s) 15, 64, 73, 2132, 2179, 2237
BstSFI CTRYAG 4 cut(s) 687, 1101, 1676, 2070
BstUI CGCG 1 cut(s) 87
BstV2I GAAGAC 1 cut(s) 333
BstX2I RGATCY 2 cut(s) 406, 760
BstYI RGATCY 2 cut(s) 406, 760
Bsu15I ATCGAT 1 cut(s) 2258
Bsu36I CCTNAGG 2 cut(s) 27, 635
BsuRI GGCC 7 cut(s) 72, 144, 590, 1377, 1383, 1631, 1746
BsuTUI ATCGAT 1 cut(s) 2258
BtgI CCRYGG 3 cut(s) 984, 1378, 1485
BtsCI GGATG 5 cut(s) 40, 179, 1525, 2026, 2205
BtsI GCAGTG 3 cut(s) 316, 842, 2079
BtsIMutI CAGTG 4 cut(s) 316, 428, 842, 2079
BveI ACCTGC 1 cut(s) 1325
Cac8I GCNNGC 7 cut(s) 375, 588, 994, 1092, 1311, 1394, 1605
CaiI CAGNNNCTG 2 cut(s) 824, 1100
CfoI GCGC 3 cut(s) 25, 464, 1906
Cfr10I RCCGGY 1 cut(s) 1463
Cfr13I GGNCC 8 cut(s) 71, 143, 529, 1421, 1744, 2130, 2235, 2510
ClaI ATCGAT 1 cut(s) 2258
Csp6I GTAC 5 cut(s) 213, 347, 1461, 1751, 1824
CspAI ACCGGT 1 cut(s) 1463
CviQI GTAC 5 cut(s) 213, 347, 1461, 1751, 1824
DdeI CTNAG 6 cut(s) 27, 635, 979, 1300, 1554, 2144
EaeI YGGCCR 1 cut(s) 1375
Eam1104I CTCTTC 1 cut(s) 1572
EarI CTCTTC 1 cut(s) 1572
EciI GGCGGA 2 cut(s) 1832, 2424
Ecl136II GAGCTC 2 cut(s) 611, 1652
Eco130I CCWWGG 6 cut(s) 280, 591, 1173, 1378, 1485, 2513
Eco147I AGGCCT 1 cut(s) 1631
Eco24I GRGCYC 3 cut(s) 613, 1654, 2458
Eco47I GGWCC 5 cut(s) 529, 1421, 2130, 2235, 2510
Eco53kI GAGCTC 2 cut(s) 611, 1652
Eco57I CTGAAG 1 cut(s) 214
Eco81I CCTNAGG 2 cut(s) 27, 635
Eco88I CYCGRG 2 cut(s) 752, 2506
EcoICRI GAGCTC 2 cut(s) 611, 1652
EcoNI CCTNNNNNAGG 1 cut(s) 1185
EcoO109I RGGNCCY 1 cut(s) 2510
EcoRII CCWGG 2 cut(s) 64, 73
EcoT14I CCWWGG 6 cut(s) 280, 591, 1173, 1378, 1485, 2513
EcoT22I ATGCAT 1 cut(s) 1394
EcoT38I GRGCYC 3 cut(s) 613, 1654, 2458
ErhI CCWWGG 6 cut(s) 280, 591, 1173, 1378, 1485, 2513
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 1616, 1648
FaqI GGGAC 3 cut(s) 806, 1470, 1928
FauI CCCGC 1 cut(s) 69
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 1 cut(s) 1322
FokI GGATG 5 cut(s) 47, 186, 1532, 2033, 2212
FriOI GRGCYC 3 cut(s) 613, 1654, 2458
FspI TGCGCA 1 cut(s) 1905
GlaI GCGC 3 cut(s) 24, 463, 1905
GsaI CCCAGC 2 cut(s) 1032, 2502
GsuI CTGGAG 3 cut(s) 801, 1160, 1347
HaeII RGCGCY 2 cut(s) 26, 465
HaeIII GGCC 7 cut(s) 72, 144, 590, 1377, 1383, 1631, 1746
HapII CCGG 5 cut(s) 17, 1464, 2133, 2180, 2238
HhaI GCGC 3 cut(s) 25, 464, 1906
Hin6I GCGC 3 cut(s) 23, 462, 1904
HinP1I GCGC 3 cut(s) 23, 462, 1904
HincII GTYRAC 1 cut(s) 1371
HindII GTYRAC 1 cut(s) 1371
HpaII CCGG 5 cut(s) 17, 1464, 2133, 2180, 2238
HphI GGTGA 2 cut(s) 2219, 2387
Hpy166II GTNNAC 4 cut(s) 1019, 1323, 1371, 1880
Hpy188I TCNGA 8 cut(s) 233, 255, 504, 800, 1741, 2062, 2157, 2255
Hpy8I GTNNAC 4 cut(s) 1019, 1323, 1371, 1880
Hpy99I CGWCG 4 cut(s) 1647, 1650, 2316, 2408
HpyCH4III ACNGT 4 cut(s) 130, 346, 1686, 2297
HpyCH4IV ACGT 3 cut(s) 1459, 1642, 1876
HpyF3I CTNAG 6 cut(s) 27, 635, 979, 1300, 1554, 2144
HpySE526I ACGT 3 cut(s) 1459, 1642, 1876
HspAI GCGC 3 cut(s) 23, 462, 1904
Ksp22I TGATCA 1 cut(s) 499
LmnI GCTCC 6 cut(s) 457, 608, 616, 1006, 1709, 2157
LweI GCATC 6 cut(s) 836, 924, 1255, 1372, 1450, 2182
MaeII ACGT 3 cut(s) 1459, 1642, 1876
MaeIII GTNAC 3 cut(s) 836, 1686, 2375
MfeI CAATTG 1 cut(s) 1122
MflI RGATCY 2 cut(s) 406, 760
MhlI GDGCHC 3 cut(s) 613, 1654, 2458
MlsI TGGCCA 1 cut(s) 1377
MluNI TGGCCA 1 cut(s) 1377
MlyI GAGTC 3 cut(s) 503, 666, 1328
Mox20I TGGCCA 1 cut(s) 1377
Mph1103I ATGCAT 1 cut(s) 1394
MroXI GAANNNNTTC 2 cut(s) 1581, 1803
MscI TGGCCA 1 cut(s) 1377
MseI TTAA 6 cut(s) 206, 387, 1233, 1296, 1866, 2536
MslI CAYNNNNRTG 3 cut(s) 1986, 2280, 2334
Msp20I TGGCCA 1 cut(s) 1377
MspA1I CMGCKG 1 cut(s) 1100
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 5 cut(s) 17, 1464, 2133, 2180, 2238
MspR9I CCNGG 6 cut(s) 17, 66, 75, 2134, 2181, 2239
MunI CAATTG 1 cut(s) 1122
Mva1269I GAATGC 1 cut(s) 1288
MvaI CCWGG 2 cut(s) 66, 75
MvnI CGCG 1 cut(s) 87
NciI CCSGG 4 cut(s) 17, 2134, 2181, 2239
NcoI CCATGG 2 cut(s) 1378, 1485
NmuCI GTSAC 1 cut(s) 2375
NsbI TGCGCA 1 cut(s) 1905
NsiI ATGCAT 1 cut(s) 1394
NspI RCATGY 3 cut(s) 770, 1241, 1396
NspV TTCGAA 1 cut(s) 401
PaeI GCATGC 1 cut(s) 1396
PaqCI CACCTGC 1 cut(s) 1325
PceI AGGCCT 1 cut(s) 1631
PciI ACATGT 2 cut(s) 766, 1237
PcsI WCGNNNNNNNCGW 2 cut(s) 370, 2400
PctI GAATGC 1 cut(s) 1288
PdmI GAANNNNTTC 2 cut(s) 1581, 1803
PfeI GAWTC 9 cut(s) 101, 672, 707, 851, 964, 1490, 2063, 2174, 2412
PfoI TCCNGGA 1 cut(s) 15
PinAI ACCGGT 1 cut(s) 1463
PleI GAGTC 3 cut(s) 503, 665, 1327
PpsI GAGTC 3 cut(s) 503, 665, 1327
PpuMI RGGWCCY 1 cut(s) 2510
PscI ACATGT 2 cut(s) 766, 1237
PshAI GACNNNNGTC 1 cut(s) 2298
PsiI TTATAA 1 cut(s) 1130
Psp124BI GAGCTC 2 cut(s) 613, 1654
Psp5II RGGWCCY 1 cut(s) 2510
Psp6I CCWGG 2 cut(s) 64, 73
PspFI CCCAGC 2 cut(s) 1028, 2498
PspGI CCWGG 2 cut(s) 64, 73
PspPI GGNCC 8 cut(s) 71, 143, 529, 1421, 1744, 2130, 2235, 2510
PspPPI RGGWCCY 1 cut(s) 2510
PsrI GAACNNNNNNTAC 2 cut(s) 1566, 1598
PstI CTGCAG 3 cut(s) 691, 1105, 2074
PstNI CAGNNNCTG 2 cut(s) 824, 1100
PsuI RGATCY 2 cut(s) 406, 760
PvuII CAGCTG 1 cut(s) 1100
RsaI GTAC 5 cut(s) 214, 348, 1462, 1752, 1825
RsaNI GTAC 5 cut(s) 213, 347, 1461, 1751, 1824
RseI CAYNNNNRTG 3 cut(s) 1986, 2280, 2334
SacI GAGCTC 2 cut(s) 613, 1654
SaqAI TTAA 6 cut(s) 206, 387, 1233, 1296, 1866, 2536
Sau96I GGNCC 8 cut(s) 71, 143, 529, 1421, 1744, 2130, 2235, 2510
SchI GAGTC 3 cut(s) 503, 666, 1328
ScrFI CCNGG 6 cut(s) 17, 66, 75, 2134, 2181, 2239
SduI GDGCHC 3 cut(s) 613, 1654, 2458
SfaNI GCATC 6 cut(s) 836, 924, 1255, 1372, 1450, 2182
SfcI CTRYAG 4 cut(s) 687, 1101, 1676, 2070
SfuI TTCGAA 1 cut(s) 401
SinI GGWCC 5 cut(s) 529, 1421, 2130, 2235, 2510
SmiMI CAYNNNNRTG 3 cut(s) 1986, 2280, 2334
SmlI CTYRAG 3 cut(s) 386, 1116, 1793
SmoI CTYRAG 3 cut(s) 386, 1116, 1793
SphI GCATGC 1 cut(s) 1396
SseBI AGGCCT 1 cut(s) 1631
SstI GAGCTC 2 cut(s) 613, 1654
StuI AGGCCT 1 cut(s) 1631
StyD4I CCNGG 6 cut(s) 15, 64, 73, 2132, 2179, 2237
StyI CCWWGG 6 cut(s) 280, 591, 1173, 1378, 1485, 2513
TaaI ACNGT 4 cut(s) 130, 346, 1686, 2297
TaiI ACGT 3 cut(s) 1462, 1645, 1879
TaqI TCGA 6 cut(s) 401, 712, 967, 1044, 1648, 2258
TaqII GACCGA 1 cut(s) 2209
TatI WGTACW 1 cut(s) 346
TauI GCSGC 1 cut(s) 72
TfiI GAWTC 9 cut(s) 101, 672, 707, 851, 964, 1490, 2063, 2174, 2412
Tru1I TTAA 6 cut(s) 206, 387, 1233, 1296, 1866, 2536
Tru9I TTAA 6 cut(s) 206, 387, 1233, 1296, 1866, 2536
TscAI CASTG 4 cut(s) 316, 435, 849, 2079
TseFI GTSAC 1 cut(s) 2375
Tsp45I GTSAC 1 cut(s) 2375
TspDTI ATGAA 4 cut(s) 429, 1536, 1788, 2318
TspRI CASTG 4 cut(s) 316, 435, 849, 2079
Vha464I CTTAAG 1 cut(s) 386
VpaK11BI GGWCC 5 cut(s) 529, 1421, 2130, 2235, 2510
XagI CCTNNNNNAGG 1 cut(s) 1185
XapI RAATTY 1 cut(s) 271
XbaI TCTAGA 2 cut(s) 1343, 1612
XceI RCATGY 3 cut(s) 770, 1241, 1396
XcmI CCANNNNNNNNNTGG 1 cut(s) 62
XmiI GTMKAC 1 cut(s) 1322
XmnI GAANNNNTTC 2 cut(s) 1581, 1803
Zsp2I ATGCAT 1 cut(s) 1394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.