AT4G25315

Protein of unknown function (DUF3128)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010339
Physical Location & Seq
Reverse (-)
12951109 .. 12952952
1844 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G25315.2

Sequence Viewer

Length: 384 bp
ATGAGCTCGGAGAACGAAGACTCTTCATCTGTGCGACGGCGCGTGTCCTGCACTAAATGCTTCGACGCTCTCTGGTTCTGCTATTGGATGTCTAGATGGTCCAACATAACCAGCAATGGCGATGACACAGGAAACGATTTGGCACCATTTTATCAAATGCAGCAGTATTACCGGGTTGGAAAACTTGATGATTGTACTAAGAAGTTCTCTGACCTCTTTGATTGTCTTTCCTTGAAGACAAAAAGAGCTTCTGAAGCCGAGAGAATTCTGGAAGAGCAAGAGAAAGCTGCTGCGGAAAAACATATCTGGATTATGCGAACACAGGAAGAAGCTGTAAGCCATTGGAATGAGACTTTTGGGCATTTGGAAGATCCAAATTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3128

Protein Analysis

127

Amino Acids

14.91

Weight (kDa)

5.05

Isoelectric Point (pI)

78.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PANTS-like PF11326 15 - 87 2.9e-19 Synaptic plasticity regulator PANTS-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010339)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25315 AT4G25315 AT4G25315
fragaria_vesca FvH4_3g30110
malus_domestica MD03G1133000.v1.1 MD14G1038500.v1.1
prunus_persica Prupe.6G117700_v2.0.a1
pyrus_communis pycom14g03310
rosa_chinensis RchiOBHm_Chr5g0055601
rosa_laevigata RLG00000034999
rosa_multiflora Rmu_sc0004818.1_g000002
rosa_roxburghii Rroxscaffold_1G00024680
rosa_rugosa Rorug05G0294200 Rorug05G0294300 Rorug05G0294300 Rorug05G0294400
rosa_samantha Rh5AG363600 Rh5BG375700 Rh5CG398200 Rh5DG388900
rosa_wichuraiana Rw5G034210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 142
AccII CGCG 1 cut(s) 42
AciI CCGC 1 cut(s) 293
AclWI GGATC 1 cut(s) 365
AcsI RAATTY 2 cut(s) 264, 376
AcuI CTGAAG 1 cut(s) 273
AfaI GTAC 1 cut(s) 196
AgsI TTSAA 1 cut(s) 235
AluBI AGCT 4 cut(s) 6, 248, 287, 332
AluI AGCT 4 cut(s) 6, 248, 287, 332
Alw21I GWGCWC 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 344
AlwI GGATC 1 cut(s) 365
ApeKI GCWGC 3 cut(s) 160, 287, 290
ApoI RAATTY 2 cut(s) 264, 376
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 1 cut(s) 99
AsuC2I CCSGG 1 cut(s) 173
AvaII GGWCC 1 cut(s) 99
BanI GGYRCC 1 cut(s) 142
BanII GRGCYC 1 cut(s) 8
BbsI GAAGAC 2 cut(s) 24, 242
Bbv12I GWGCWC 1 cut(s) 8
BbvI GCAGC 3 cut(s) 172, 274, 277
BccI CCATC 1 cut(s) 90
BceAI ACGGC 1 cut(s) 53
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 1 cut(s) 344
BfaI CTAG 1 cut(s) 93
BisI GCNGC 3 cut(s) 161, 288, 291
BlsI GCNGC 3 cut(s) 162, 289, 292
Bme1390I CCNGG 1 cut(s) 173
Bme18I GGWCC 1 cut(s) 99
BmgT120I GGNCC 1 cut(s) 99
BmiI GGNNCC 1 cut(s) 144
BmrFI CCNGG 1 cut(s) 173
BpiI GAAGAC 2 cut(s) 24, 242
BpuMI CCSGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 121
BseGI GGATG 1 cut(s) 93
BseMI GCAATG 1 cut(s) 121
BseXI GCAGC 3 cut(s) 172, 274, 277
BsgI GTGCAG 1 cut(s) 34
Bsh1236I CGCG 1 cut(s) 42
BshNI GGYRCC 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 8
BsiSI CCGG 1 cut(s) 172
BsmAI GTCTC 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 8
Bsp143I GATC 1 cut(s) 370
BspACI CCGC 1 cut(s) 293
BspFNI CGCG 1 cut(s) 42
BspLI GGNNCC 1 cut(s) 144
BspPI GGATC 1 cut(s) 365
BspQI GCTCTTC 1 cut(s) 267
BspT107I GGYRCC 1 cut(s) 142
BsrDI GCAATG 1 cut(s) 121
BssMI GATC 1 cut(s) 370
Bst6I CTCTTC 2 cut(s) 28, 267
BstAPI GCANNNNNTGC 1 cut(s) 57
BstDEI CTNAG 2 cut(s) 198, 381
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 1 cut(s) 42
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 1 cut(s) 373
BstMAI GTCTC 1 cut(s) 344
BstMBI GATC 1 cut(s) 370
BstMWI GCNNNNNNNGC 3 cut(s) 48, 57, 254
BstSCI CCNGG 1 cut(s) 171
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 3 cut(s) 172, 274, 277
BstV2I GAAGAC 2 cut(s) 24, 242
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BtgZI GCGATG 1 cut(s) 135
BtsCI GGATG 1 cut(s) 93
CfoI GCGC 1 cut(s) 42
Cfr13I GGNCC 1 cut(s) 99
CseI GACGC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 195
CviJI RGCY 6 cut(s) 6, 248, 257, 287, 332, 339
CviKI_1 RGCY 6 cut(s) 6, 248, 257, 287, 332, 339
CviQI GTAC 1 cut(s) 195
DdeI CTNAG 2 cut(s) 198, 381
DpnI GATC 1 cut(s) 372
DpnII GATC 1 cut(s) 370
Eam1104I CTCTTC 2 cut(s) 28, 267
EarI CTCTTC 2 cut(s) 28, 267
Ecl136II GAGCTC 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 99
Eco53kI GAGCTC 1 cut(s) 6
Eco57I CTGAAG 1 cut(s) 273
EcoICRI GAGCTC 1 cut(s) 6
EcoRI GAATTC 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 8
FaiI YATR 3 cut(s) 107, 303, 314
Fnu4HI GCNGC 3 cut(s) 161, 288, 291
FokI GGATG 1 cut(s) 100
FriOI GRGCYC 1 cut(s) 8
Fsp4HI GCNGC 3 cut(s) 161, 288, 291
FspBI CTAG 1 cut(s) 93
GlaI GCGC 1 cut(s) 41
GluI GCNGC 3 cut(s) 161, 288, 291
HapII CCGG 1 cut(s) 172
HgaI GACGC 1 cut(s) 74
HhaI GCGC 1 cut(s) 42
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HinfI GANTC 1 cut(s) 20
HpaII CCGG 1 cut(s) 172
Hpy188I TCNGA 3 cut(s) 10, 211, 253
Hpy188III TCNNGA 3 cut(s) 93, 269, 307
Hpy99I CGWCG 2 cut(s) 39, 68
HpyCH4V TGCA 2 cut(s) 51, 160
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 57, 254
HpyF3I CTNAG 2 cut(s) 198, 381
HspAI GCGC 1 cut(s) 40
Kzo9I GATC 1 cut(s) 370
LguI GCTCTTC 1 cut(s) 267
LpnPI CCDG 8 cut(s) 58, 61, 114, 124, 185, 254, 292, 308
Lsp1109I GCAGC 3 cut(s) 172, 274, 277
MaeI CTAG 1 cut(s) 93
MalI GATC 1 cut(s) 372
MboI GATC 1 cut(s) 370
MboII GAAGA 6 cut(s) 15, 29, 247, 284, 338, 380
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 1 cut(s) 8
MluCI AATT 2 cut(s) 264, 376
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 126, 157
MnlI CCTC 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 345
MspI CCGG 1 cut(s) 172
MspR9I CCNGG 1 cut(s) 173
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 3 cut(s) 48, 57, 254
NciI CCSGG 1 cut(s) 173
NdeII GATC 1 cut(s) 370
NlaIV GGNNCC 1 cut(s) 144
NmeAIII GCCGAG 1 cut(s) 283
PciSI GCTCTTC 1 cut(s) 267
PkrI GCNGC 3 cut(s) 162, 289, 292
PleI GAGTC 1 cut(s) 14
PpsI GAGTC 1 cut(s) 14
Psp124BI GAGCTC 1 cut(s) 8
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 99
PsuI RGATCY 1 cut(s) 370
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 267
SatI GCNGC 3 cut(s) 161, 288, 291
Sau3AI GATC 1 cut(s) 370
Sau96I GGNCC 1 cut(s) 99
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 1 cut(s) 173
SduI GDGCHC 1 cut(s) 8
SetI ASST 5 cut(s) 8, 216, 250, 289, 334
SinI GGWCC 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 345
Sse9I AATT 2 cut(s) 264, 376
SsiI CCGC 1 cut(s) 293
SspMI CTAG 1 cut(s) 93
SstI GAGCTC 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 171
TaqI TCGA 1 cut(s) 63
TasI AATT 2 cut(s) 264, 376
TatI WGTACW 1 cut(s) 194
TseI GCWGC 3 cut(s) 160, 287, 290
TspDTI ATGAA 1 cut(s) 15
VpaK11BI GGWCC 1 cut(s) 99
XapI RAATTY 2 cut(s) 264, 376
XbaI TCTAGA 1 cut(s) 92
XspI CTAG 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.