Rorug05G0294300

Protein of unknown function (DUF3128)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010339
Physical Location & Seq
Forward (+)
33941689 .. 33945299
3611 bp
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UTR
Exon/CDS
Intron
Rorug05G0294300.1

Sequence Viewer

Length: 699 bp
ATGGCATCTTCTTCGGACTCATGGATGAAGGAGTATAATGAAGCAGTAAAACTTGCTGATGATATCGGTGGTATGATATCTGAACGGAGTTCATTTCCCGCATCAGGTCCAGAAACTCAGCGTCATGTCTCTGCTATTCGGAGGAAAGTTACAATATTGGGGACTAGACTTGATAGCTTACAGTCCGAATTGTCAACACTTCCTGGAAAGCAGCCCATAACAGCCAAAGAGATGAATCGTCGGAAGGACATGGTTGCAAATTTGAGATCAAAAGTTAACCAGATGTCTTCAACGTTGACATCAAACCCTGGTAACAGAGACAGCTTGATTGGACCTGAAATAAGTAAAGCTGATGCCATGGGCCGAGCAGCTGGCCTGGACAACTATGGCCTTGTTGGTCTTCAGCGACAAGTTATGAAAGAGCAAGATGAGGGGCTTGACAAATTGGAGGAGACTGTAGTAAGCACAAAACATATTGCATTGGCAGTCAACGAGGAACTCACCCTGCACACTAGGCTTATTGATGATTTGGATGAACATGTGGATGTTACTAACTCCCGGCTACGGCGAGTGCAGAAGAACTTGGCAGCTTTGAACAAACGCACAAAGGGTGGTTGCTCGTGTATGTGCATGCTTTTAGCTGTTCTCGGTATTGTGGGCCTGGTTGCTGTGATATTTTTGCTGATCAAATACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3128

Protein Analysis

232

Amino Acids

25.45

Weight (kDa)

8.74

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNARE PF05739 173 - 223 9.7e-11 SNARE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010339)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G25315 AT4G25315 AT4G25315
fragaria_vesca FvH4_3g30110
malus_domestica MD03G1133000.v1.1 MD14G1038500.v1.1
prunus_persica Prupe.6G117700_v2.0.a1
pyrus_communis pycom14g03310
rosa_chinensis RchiOBHm_Chr5g0055601
rosa_laevigata RLG00000034999
rosa_multiflora Rmu_sc0004818.1_g000002
rosa_roxburghii Rroxscaffold_1G00024680
rosa_rugosa Rorug05G0294200 Rorug05G0294300 Rorug05G0294300 Rorug05G0294400
rosa_samantha Rh5AG363600 Rh5BG375700 Rh5CG398200 Rh5DG388900
rosa_wichuraiana Rw5G034210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 99
AclI AACGTT 1 cut(s) 293
AcsI RAATTY 1 cut(s) 259
AcuI CTGAAG 1 cut(s) 386
AfiI CCNNNNNNNGG 2 cut(s) 104, 564
AflIII ACRYGT 1 cut(s) 538
AgsI TTSAA 2 cut(s) 291, 595
AjnI CCWGG 4 cut(s) 202, 307, 375, 660
AluBI AGCT 6 cut(s) 177, 324, 350, 371, 590, 641
AluI AGCT 6 cut(s) 177, 324, 350, 371, 590, 641
Alw26I GTCTC 3 cut(s) 133, 312, 446
AoxI GGCC 4 cut(s) 361, 373, 388, 658
ApeKI GCWGC 3 cut(s) 211, 368, 587
ApoI RAATTY 1 cut(s) 259
AspS9I GGNCC 4 cut(s) 107, 332, 361, 658
AsuC2I CCSGG 1 cut(s) 559
AsuHPI GGTGA 1 cut(s) 493
AvaII GGWCC 2 cut(s) 107, 332
BauI CACGAG 1 cut(s) 619
BbsI GAAGAC 2 cut(s) 279, 392
BbvI GCAGC 3 cut(s) 223, 380, 599
BceAI ACGGC 1 cut(s) 581
BcgI CGANNNNNNTGC 1 cut(s) 28
BciT130I CCWGG 4 cut(s) 204, 309, 377, 662
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 1 cut(s) 559
BcoDI GTCTC 3 cut(s) 133, 312, 446
BfaI CTAG 2 cut(s) 165, 513
BfmI CTRYAG 1 cut(s) 456
BisI GCNGC 3 cut(s) 212, 369, 588
BlsI GCNGC 3 cut(s) 213, 370, 589
Bme1390I CCNGG 5 cut(s) 204, 309, 377, 559, 662
Bme18I GGWCC 2 cut(s) 107, 332
BmgT120I GGNCC 4 cut(s) 107, 332, 361, 658
BmrFI CCNGG 5 cut(s) 204, 309, 377, 559, 662
BmsI GCATC 3 cut(s) 14, 110, 343
BpiI GAAGAC 2 cut(s) 279, 392
BpuMI CCSGG 1 cut(s) 559
BsaJI CCNNGG 2 cut(s) 307, 357
Bsc4I CCNNNNNNNGG 2 cut(s) 104, 564
BseBI CCWGG 4 cut(s) 204, 309, 377, 662
BseDI CCNNGG 2 cut(s) 307, 357
BseGI GGATG 3 cut(s) 30, 538, 550
BseLI CCNNNNNNNGG 2 cut(s) 104, 564
BseMII CTCAG 1 cut(s) 131
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 3 cut(s) 223, 380, 599
BsgI GTGCAG 2 cut(s) 491, 593
BshFI GGCC 4 cut(s) 363, 375, 390, 660
BsiSI CCGG 1 cut(s) 559
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 2 cut(s) 104, 564
BsmAI GTCTC 3 cut(s) 133, 312, 446
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 4 cut(s) 363, 375, 390, 660
Bsp143I GATC 2 cut(s) 266, 684
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 1 cut(s) 99
BspANI GGCC 4 cut(s) 363, 375, 390, 660
BspCNI CTCAG 1 cut(s) 130
BssECI CCNNGG 2 cut(s) 307, 357
BssMI GATC 2 cut(s) 266, 684
BssSI CACGAG 1 cut(s) 619
BssT1I CCWWGG 1 cut(s) 357
Bst2BI CACGAG 1 cut(s) 619
Bst2UI CCWGG 4 cut(s) 204, 309, 377, 662
Bst4CI ACNGT 2 cut(s) 183, 457
BstC8I GCNNGC 2 cut(s) 373, 632
BstDEI CTNAG 1 cut(s) 117
BstDSI CCRYGG 1 cut(s) 357
BstF5I GGATG 3 cut(s) 30, 538, 550
BstKTI GATC 2 cut(s) 269, 687
BstMAI GTCTC 3 cut(s) 133, 312, 446
BstMBI GATC 2 cut(s) 266, 684
BstMWI GCNNNNNNNGC 1 cut(s) 514
BstNI CCWGG 4 cut(s) 204, 309, 377, 662
BstNSI RCATGY 2 cut(s) 542, 634
BstSCI CCNGG 5 cut(s) 202, 307, 375, 557, 660
BstSFI CTRYAG 1 cut(s) 456
BstV1I GCAGC 3 cut(s) 223, 380, 599
BstV2I GAAGAC 2 cut(s) 279, 392
BsuRI GGCC 4 cut(s) 363, 375, 390, 660
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 3 cut(s) 30, 538, 550
Cac8I GCNNGC 2 cut(s) 373, 632
Cfr13I GGNCC 4 cut(s) 107, 332, 361, 658
CseI GACGC 1 cut(s) 110
CviAII CATG 6 cut(s) 21, 125, 250, 358, 539, 631
DdeI CTNAG 1 cut(s) 117
DpnI GATC 2 cut(s) 268, 686
DpnII GATC 2 cut(s) 266, 684
Eco130I CCWWGG 1 cut(s) 357
Eco32I GATATC 2 cut(s) 64, 78
Eco47I GGWCC 2 cut(s) 107, 332
Eco57I CTGAAG 1 cut(s) 386
EcoRII CCWGG 4 cut(s) 202, 307, 375, 660
EcoRV GATATC 2 cut(s) 64, 78
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 6 cut(s) 24, 128, 253, 361, 542, 634
FaqI GGGAC 1 cut(s) 175
FatI CATG 6 cut(s) 20, 124, 249, 357, 538, 630
FauI CCCGC 1 cut(s) 106
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 3 cut(s) 212, 369, 588
FokI GGATG 3 cut(s) 37, 545, 557
Fsp4HI GCNGC 3 cut(s) 212, 369, 588
FspBI CTAG 2 cut(s) 165, 513
GluI GCNGC 3 cut(s) 212, 369, 588
HaeIII GGCC 4 cut(s) 363, 375, 390, 660
HapII CCGG 1 cut(s) 559
HgaI GACGC 1 cut(s) 110
Hin1II CATG 6 cut(s) 24, 128, 253, 361, 542, 634
HincII GTYRAC 4 cut(s) 195, 277, 297, 490
HindII GTYRAC 4 cut(s) 195, 277, 297, 490
HinfI GANTC 2 cut(s) 17, 235
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 1 cut(s) 559
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 4 cut(s) 195, 277, 297, 490
Hpy188I TCNGA 5 cut(s) 16, 82, 141, 187, 243
Hpy188III TCNNGA 1 cut(s) 110
Hpy8I GTNNAC 4 cut(s) 195, 277, 297, 490
Hpy99I CGWCG 1 cut(s) 243
HpyAV CCTTC 2 cut(s) 22, 238
HpyCH4III ACNGT 2 cut(s) 183, 457
HpyCH4IV ACGT 1 cut(s) 293
HpyCH4V TGCA 5 cut(s) 257, 479, 508, 574, 630
HpyF10VI GCNNNNNNNGC 1 cut(s) 514
HpyF3I CTNAG 1 cut(s) 117
HpySE526I ACGT 1 cut(s) 293
Hsp92II CATG 6 cut(s) 24, 128, 253, 361, 542, 634
Ksp22I TGATCA 1 cut(s) 684
KspAI GTTAAC 1 cut(s) 277
Kzo9I GATC 2 cut(s) 266, 684
Lsp1109I GCAGC 3 cut(s) 223, 380, 599
LweI GCATC 3 cut(s) 14, 110, 343
MaeI CTAG 2 cut(s) 165, 513
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 3 cut(s) 148, 311, 547
MalI GATC 2 cut(s) 268, 686
MboI GATC 2 cut(s) 266, 684
MboII GAAGA 4 cut(s) 3, 279, 392, 589
MluCI AATT 3 cut(s) 188, 259, 443
MlyI GAGTC 1 cut(s) 11
MmeI TCCRAC 1 cut(s) 221
MnlI CCTC 4 cut(s) 135, 424, 442, 487
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 371
MspI CCGG 1 cut(s) 559
MspR9I CCNGG 5 cut(s) 204, 309, 377, 559, 662
MvaI CCWGG 4 cut(s) 204, 309, 377, 662
MwoI GCNNNNNNNGC 1 cut(s) 514
NciI CCSGG 1 cut(s) 559
NcoI CCATGG 1 cut(s) 357
NdeII GATC 2 cut(s) 266, 684
NlaIII CATG 6 cut(s) 24, 128, 253, 361, 542, 634
NmeAIII GCCGAG 1 cut(s) 389
NspI RCATGY 2 cut(s) 542, 634
PaeI GCATGC 1 cut(s) 634
PciI ACATGT 1 cut(s) 538
PfeI GAWTC 1 cut(s) 235
PfoI TCCNGGA 1 cut(s) 202
PkrI GCNGC 3 cut(s) 213, 370, 589
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
PscI ACATGT 1 cut(s) 538
Psp1406I AACGTT 1 cut(s) 293
Psp6I CCWGG 4 cut(s) 202, 307, 375, 660
PspGI CCWGG 4 cut(s) 202, 307, 375, 660
PspPI GGNCC 4 cut(s) 107, 332, 361, 658
PvuII CAGCTG 1 cut(s) 371
RseI CAYNNNNRTG 1 cut(s) 543
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 3 cut(s) 212, 369, 588
Sau3AI GATC 2 cut(s) 266, 684
Sau96I GGNCC 4 cut(s) 107, 332, 361, 658
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 5 cut(s) 204, 309, 377, 559, 662
SetI ASST 9 cut(s) 109, 179, 296, 326, 337, 352, 373, 592, 643
SfaNI GCATC 3 cut(s) 14, 110, 343
SfcI CTRYAG 1 cut(s) 456
SinI GGWCC 2 cut(s) 107, 332
SmiMI CAYNNNNRTG 1 cut(s) 543
SphI GCATGC 1 cut(s) 634
Sse9I AATT 3 cut(s) 188, 259, 443
SsiI CCGC 1 cut(s) 99
SspI AATATT 1 cut(s) 156
SspMI CTAG 2 cut(s) 165, 513
StyD4I CCNGG 5 cut(s) 202, 307, 375, 557, 660
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 2 cut(s) 183, 457
TaiI ACGT 1 cut(s) 296
TasI AATT 3 cut(s) 188, 259, 443
TfiI GAWTC 1 cut(s) 235
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TseI GCWGC 3 cut(s) 211, 368, 587
TspDTI ATGAA 6 cut(s) 41, 54, 81, 248, 431, 549
TspGWI ACGGA 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 107, 332
XapI RAATTY 1 cut(s) 259
XceI RCATGY 2 cut(s) 542, 634
XspI CTAG 2 cut(s) 165, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.