AT4G26160

Thioredoxin-like 2-1, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009979
Physical Location & Seq
Forward (+)
13255251 .. 13256879
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G26160.1

Sequence Viewer

Length: 666 bp
ATGTCTCCGACGACGACATCGTTACGATCCCTAAGCTTCTCGCTTTACGCTTCTTCTAATTCTACTCCGATTTCTACACCAATTGAGGCCCGACAGTTACTGAGTTCATGCAATAGATTCTACGGTCTGTCCTCTTCGAGCTCTTCTTCCTCCCTTACTACTTCATCTTTGATCGGAAATCTTGTTTTCTCATCAAGGAATCAGTCTCTCTCCGTAAAGGTCCAAGCGTTAGCTGCTGAAACCGAACAGCCAAAATGGTGGGAGAGGAAAGCAGGGCCAAACATGATTGACATTACCTCAGCTGAGCAGTTTCTCAACGCGTTAAAAGATGCGGGAGATAGATTAGTTATCGTAGATTTTTACGGAACTTGGTGTGGTTCTTGCCGTGCAATGTTCCCAAAGCTCTGCAAAACGGCGAAAGAACACCCTAACATCTTGTTCCTTAAAGTAAACTTTGACGAGAACAAGTCTCTCTGCAAAAGCTTAAACGTCAAGGTGTTACCGTATTTCCACTTCTACCGCGGTGCTGATGGCCAAGTCGAATCCTTCTCATGCTCTCTTGCCAAGTTCCAGAAACTGAGAGAGGCGATAGAGAGACACAATGTAGGAAGTATCAGTAACATCTCTTCTTCAGCTTCTGAGAAAGTTGAAGATTCAAGTGAATAA

Protein Analysis

221

Amino Acids

24.35

Weight (kDa)

8.72

Isoelectric Point (pI)

53.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 99 - 186 6.4e-20 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 320, 522
AciI CCGC 3 cut(s) 332, 520, 522
AclWI GGATC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 532
AcuI CTGAAG 1 cut(s) 615
AflIII ACRYGT 1 cut(s) 318
AgsI TTSAA 2 cut(s) 650, 657
AluBI AGCT 7 cut(s) 36, 141, 233, 302, 403, 483, 635
AluI AGCT 7 cut(s) 36, 141, 233, 302, 403, 483, 635
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 4 cut(s) 9, 210, 474, 589
AlwI GGATC 1 cut(s) 21
AlwNI CAGNNNCTG 3 cut(s) 100, 577, 638
AoxI GGCC 3 cut(s) 87, 275, 532
ApeKI GCWGC 1 cut(s) 233
AspS9I GGNCC 3 cut(s) 88, 220, 275
AvaII GGWCC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 534
BanII GRGCYC 1 cut(s) 143
BarI GAAGNNNNNNTAC 4 cut(s) 497, 529, 610, 642
Bbv12I GWGCWC 1 cut(s) 143
BbvCI CCTCAGC 1 cut(s) 298
BbvI GCAGC 1 cut(s) 220
BccI CCATC 1 cut(s) 524
BceAI ACGGC 2 cut(s) 369, 429
BcoDI GTCTC 4 cut(s) 9, 210, 474, 589
BisI GCNGC 1 cut(s) 234
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 1 cut(s) 235
Bme18I GGWCC 1 cut(s) 220
BmgT120I GGNCC 3 cut(s) 88, 220, 275
BmsI GCATC 1 cut(s) 319
Bpu10I CCTNAGC 2 cut(s) 32, 298
Bpu1102I GCTNAGC 1 cut(s) 303
BsaJI CCNNGG 1 cut(s) 520
Bse3DI GCAATG 1 cut(s) 396
BseDI CCNNGG 1 cut(s) 520
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 5 cut(s) 92, 294, 312, 569, 630
BseXI GCAGC 1 cut(s) 220
Bsh1236I CGCG 2 cut(s) 320, 522
BshFI GGCC 3 cut(s) 89, 277, 534
BsiHKAI GWGCWC 1 cut(s) 143
BsmAI GTCTC 4 cut(s) 9, 210, 474, 589
BsnI GGCC 3 cut(s) 89, 277, 534
Bsp1286I GDGCHC 1 cut(s) 143
Bsp143I GATC 2 cut(s) 26, 171
Bsp1720I GCTNAGC 1 cut(s) 303
BspACI CCGC 3 cut(s) 332, 520, 522
BspANI GGCC 3 cut(s) 89, 277, 534
BspCNI CTCAG 5 cut(s) 93, 295, 311, 570, 631
BspFNI CGCG 2 cut(s) 320, 522
BspPI GGATC 1 cut(s) 21
BspQI GCTCTTC 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 396
BssECI CCNNGG 1 cut(s) 520
BssMI GATC 2 cut(s) 26, 171
Bst4CI ACNGT 3 cut(s) 96, 125, 504
Bst6I CTCTTC 3 cut(s) 139, 148, 631
BstDEI CTNAG 6 cut(s) 32, 101, 298, 303, 578, 639
BstDSI CCRYGG 1 cut(s) 520
BstFNI CGCG 2 cut(s) 320, 522
BstKTI GATC 2 cut(s) 29, 174
BstMAI GTCTC 4 cut(s) 9, 210, 474, 589
BstMBI GATC 2 cut(s) 26, 171
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstUI CGCG 2 cut(s) 320, 522
BstV1I GCAGC 1 cut(s) 220
BstXI CCANNNNNNTGG 1 cut(s) 258
BsuRI GGCC 3 cut(s) 89, 277, 534
BtgI CCRYGG 1 cut(s) 520
CaiI CAGNNNCTG 3 cut(s) 100, 577, 638
Cfr13I GGNCC 3 cut(s) 88, 220, 275
Cfr42I CCGCGG 1 cut(s) 523
CviAII CATG 3 cut(s) 108, 283, 552
DdeI CTNAG 6 cut(s) 32, 101, 298, 303, 578, 639
DpnI GATC 2 cut(s) 28, 173
DpnII GATC 2 cut(s) 26, 171
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 3 cut(s) 139, 148, 631
EarI CTCTTC 3 cut(s) 139, 148, 631
Ecl136II GAGCTC 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 143
Eco47I GGWCC 1 cut(s) 220
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 615
EcoICRI GAGCTC 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 143
FaeI CATG 3 cut(s) 111, 286, 555
FaiI YATR 3 cut(s) 109, 284, 553
FatI CATG 3 cut(s) 107, 282, 551
FauI CCCGC 1 cut(s) 325
Fnu4HI GCNGC 1 cut(s) 234
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 1 cut(s) 234
GluI GCNGC 1 cut(s) 234
HaeIII GGCC 3 cut(s) 89, 277, 534
Hin1II CATG 3 cut(s) 111, 286, 555
HindIII AAGCTT 2 cut(s) 34, 481
HinfI GANTC 4 cut(s) 117, 199, 542, 653
Hpy166II GTNNAC 1 cut(s) 451
Hpy188I TCNGA 4 cut(s) 9, 69, 176, 640
Hpy188III TCNNGA 1 cut(s) 571
Hpy8I GTNNAC 1 cut(s) 451
Hpy99I CGWCG 2 cut(s) 13, 16
HpyAV CCTTC 1 cut(s) 556
HpyCH4III ACNGT 3 cut(s) 96, 125, 504
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 4 cut(s) 111, 389, 408, 477
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 6 cut(s) 32, 101, 298, 303, 578, 639
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 3 cut(s) 111, 286, 555
KspI CCGCGG 1 cut(s) 523
Kzo9I GATC 2 cut(s) 26, 171
LguI GCTCTTC 1 cut(s) 148
LpnPI CCDG 2 cut(s) 258, 584
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 1 cut(s) 319
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 4 cut(s) 21, 96, 498, 617
MalI GATC 2 cut(s) 28, 173
MboI GATC 2 cut(s) 26, 171
MboII GAAGA 7 cut(s) 45, 126, 135, 138, 618, 621, 662
MfeI CAATTG 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 143
MlsI TGGCCA 1 cut(s) 534
MluCI AATT 2 cut(s) 58, 81
MluI ACGCGT 1 cut(s) 318
MluNI TGGCCA 1 cut(s) 534
MmeI TCCRAC 1 cut(s) 32
MnlI CCTC 6 cut(s) 79, 142, 160, 258, 307, 577
Mox20I TGGCCA 1 cut(s) 534
MscI TGGCCA 1 cut(s) 534
MseI TTAA 3 cut(s) 323, 444, 485
Msp20I TGGCCA 1 cut(s) 534
MspA1I CMGCKG 2 cut(s) 302, 522
MunI CAATTG 1 cut(s) 81
MvnI CGCG 2 cut(s) 320, 522
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeII GATC 2 cut(s) 26, 171
NlaIII CATG 3 cut(s) 111, 286, 555
PciSI GCTCTTC 1 cut(s) 148
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PfeI GAWTC 4 cut(s) 117, 199, 542, 653
PkrI GCNGC 1 cut(s) 235
Psp124BI GAGCTC 1 cut(s) 143
PspPI GGNCC 3 cut(s) 88, 220, 275
PstNI CAGNNNCTG 3 cut(s) 100, 577, 638
PvuII CAGCTG 1 cut(s) 302
SacI GAGCTC 1 cut(s) 143
SacII CCGCGG 1 cut(s) 523
SapI GCTCTTC 1 cut(s) 148
SaqAI TTAA 3 cut(s) 323, 444, 485
SatI GCNGC 1 cut(s) 234
Sau3AI GATC 2 cut(s) 26, 171
Sau96I GGNCC 3 cut(s) 88, 220, 275
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 1 cut(s) 319
Sfr303I CCGCGG 1 cut(s) 523
SgrBI CCGCGG 1 cut(s) 523
SinI GGWCC 1 cut(s) 220
Sse9I AATT 2 cut(s) 58, 81
SsiI CCGC 3 cut(s) 332, 520, 522
SstI GAGCTC 1 cut(s) 143
TaaI ACNGT 3 cut(s) 96, 125, 504
TaiI ACGT 1 cut(s) 492
TaqI TCGA 2 cut(s) 137, 540
TasI AATT 2 cut(s) 58, 81
TfiI GAWTC 4 cut(s) 117, 199, 542, 653
Tru1I TTAA 3 cut(s) 323, 444, 485
Tru9I TTAA 3 cut(s) 323, 444, 485
TseI GCWGC 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 96, 153
TspGWI ACGGA 2 cut(s) 202, 378
VpaK11BI GGWCC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.