Rw2G006740

Thioredoxin-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0009979
Physical Location & Seq
Forward (+)
6545559 .. 6548269
2711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006740.1

Sequence Viewer

Length: 669 bp
ATGGCTGATGTTGTTCGATTACAATTCCATTCGCTTCGGTTCTCTTCGTCTCTGCTCACCTCTTTGAATTCTCTGCAACCGGGTCTCTTTGTTAACCAAAACCCATTTAGGAGGGCCTATGCTCTCTCTGATTCTTCCCTTGCTTGCTTTGCTTACAGACCCAGAAAGCAGTTGGTACATTTCAAGGTACAGGCCGCTGTCACTGAAACAGACCAGCCAAAATGGTGGGAAAGAAGTGTTCCGAATATGATTGACATACATTCTACTCAAGAATTCTTGAGTGCTTTAGGTCAAGCTGGAGATAGATTAGTTATTGCAGAATTCTACGGCACTTGGTGTGGTTCTTGCCGTGCATTATTTCCTAAGCTCTGCAGAACAGCTGAGGATCACCCTGAGATTCTATTCCTGAAAGTGAATTTTGATGAGAATAAGCCAATGTGCAAGAGTATGAACGTGAAGGTCCTTCCCTATTTCCACTTTTACCGTGGATCTGAGGGACAGCTGGAATCCTTTTCATGTTCACTTGCTAAGTTCCAGAAAATAAAGGATGCTATTGAATTACACAACACTGCTCGTTGCAGCATCGGCCCTCCAAAGGGTGTTGGAGATCTTAAACTTGAACCTTCCTTGGTTCAGAAGGACAGACCCTCAGAATCTGCTTCAGCATAG

Protein Analysis

222

Amino Acids

25.07

Weight (kDa)

8.83

Isoelectric Point (pI)

42.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 87 - 172 5e-17 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 195
AclWI GGATC 2 cut(s) 393, 496
AcsI RAATTY 4 cut(s) 67, 272, 320, 415
AcuI CTGAAG 1 cut(s) 645
AdeI CACNNNGTG 1 cut(s) 336
AfaI GTAC 2 cut(s) 177, 189
AfiI CCNNNNNNNGG 2 cut(s) 595, 596
AgsI TTSAA 4 cut(s) 67, 184, 557, 620
AluBI AGCT 4 cut(s) 296, 367, 380, 502
AluI AGCT 4 cut(s) 296, 367, 380, 502
Alw26I GTCTC 2 cut(s) 54, 89
AlwI GGATC 2 cut(s) 393, 496
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 3 cut(s) 114, 192, 586
ApeKI GCWGC 1 cut(s) 579
ApoI RAATTY 4 cut(s) 67, 272, 320, 415
AspS9I GGNCC 3 cut(s) 114, 460, 587
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 49, 380
AvaII GGWCC 1 cut(s) 460
BbvCI CCTCAGC 1 cut(s) 381
BbvI GCAGC 1 cut(s) 591
BceAI ACGGC 2 cut(s) 333, 343
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 54, 89
BfmI CTRYAG 1 cut(s) 370
BglII AGATCT 1 cut(s) 607
BisI GCNGC 2 cut(s) 195, 580
BlsI GCNGC 2 cut(s) 196, 581
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 3 cut(s) 114, 460, 587
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 538, 591
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 2 cut(s) 363, 381
BpuEI CTTGAG 2 cut(s) 252, 298
BpuMI CCSGG 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 2 cut(s) 484, 627
Bsc4I CCNNNNNNNGG 2 cut(s) 595, 596
BseDI CCNNGG 2 cut(s) 484, 627
BseGI GGATG 1 cut(s) 553
BseLI CCNNNNNNNGG 2 cut(s) 595, 596
BseMII CTCAG 4 cut(s) 372, 384, 483, 663
BseXI GCAGC 1 cut(s) 591
BshFI GGCC 3 cut(s) 116, 194, 588
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 510
BslI CCNNNNNNNGG 2 cut(s) 595, 596
BsmAI GTCTC 2 cut(s) 54, 89
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 3 cut(s) 116, 194, 588
Bso31I GGTCTC 1 cut(s) 89
Bsp143I GATC 3 cut(s) 385, 488, 607
BspACI CCGC 1 cut(s) 195
BspANI GGCC 3 cut(s) 116, 194, 588
BspCNI CTCAG 4 cut(s) 373, 385, 484, 662
BspMAI CTGCAG 1 cut(s) 374
BspPI GGATC 2 cut(s) 393, 496
BspTNI GGTCTC 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 484, 627
BssMI GATC 3 cut(s) 385, 488, 607
BssT1I CCWWGG 1 cut(s) 627
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 6 cut(s) 363, 381, 393, 492, 528, 649
BstDSI CCRYGG 1 cut(s) 484
BstF5I GGATG 1 cut(s) 553
BstKTI GATC 3 cut(s) 388, 491, 610
BstMAI GTCTC 2 cut(s) 54, 89
BstMBI GATC 3 cut(s) 385, 488, 607
BstMWI GCNNNNNNNGC 2 cut(s) 149, 585
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 370
BstV1I GCAGC 1 cut(s) 591
BstX2I RGATCY 2 cut(s) 488, 607
BstXI CCANNNNNNTGG 1 cut(s) 225
BstYI RGATCY 2 cut(s) 488, 607
BsuRI GGCC 3 cut(s) 116, 194, 588
BtgI CCRYGG 1 cut(s) 484
BtsCI GGATG 1 cut(s) 553
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 2 cut(s) 201, 567
Cac8I GCNNGC 1 cut(s) 145
CaiI CAGNNNCTG 1 cut(s) 656
Cfr13I GGNCC 3 cut(s) 114, 460, 587
Csp6I GTAC 2 cut(s) 176, 188
CviAII CATG 1 cut(s) 516
CviQI GTAC 2 cut(s) 176, 188
DdeI CTNAG 6 cut(s) 363, 381, 393, 492, 528, 649
DpnI GATC 3 cut(s) 387, 490, 609
DpnII GATC 3 cut(s) 385, 488, 607
DraIII CACNNNGTG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 627
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 1 cut(s) 645
EcoO109I RGGNCCY 2 cut(s) 114, 460
EcoRI GAATTC 3 cut(s) 67, 272, 320
EcoT14I CCWWGG 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 627
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 1 cut(s) 519
FaiI YATR 6 cut(s) 120, 248, 257, 449, 517, 667
FaqI GGGAC 1 cut(s) 510
FatI CATG 1 cut(s) 515
Fnu4HI GCNGC 2 cut(s) 195, 580
FokI GGATG 1 cut(s) 560
Fsp4HI GCNGC 2 cut(s) 195, 580
GluI GCNGC 2 cut(s) 195, 580
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 3 cut(s) 116, 194, 588
HapII CCGG 1 cut(s) 80
Hin1II CATG 1 cut(s) 519
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 4 cut(s) 131, 397, 506, 653
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 49, 380
Hpy166II GTNNAC 2 cut(s) 94, 521
Hpy188I TCNGA 5 cut(s) 130, 243, 493, 636, 652
Hpy188III TCNNGA 4 cut(s) 269, 277, 406, 535
Hpy8I GTNNAC 2 cut(s) 94, 521
HpyAV CCTTC 4 cut(s) 451, 473, 631, 633
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 6 cut(s) 76, 317, 353, 372, 441, 579
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 585
HpyF3I CTNAG 6 cut(s) 363, 381, 393, 492, 528, 649
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 1 cut(s) 519
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 3 cut(s) 385, 488, 607
LpnPI CCDG 9 cut(s) 93, 175, 176, 227, 282, 405, 419, 488, 548
Lsp1109I GCAGC 1 cut(s) 591
LweI GCATC 2 cut(s) 538, 591
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 1 cut(s) 199
MalI GATC 3 cut(s) 387, 490, 609
MboI GATC 3 cut(s) 385, 488, 607
MboII GAAGA 2 cut(s) 36, 126
MflI RGATCY 2 cut(s) 488, 607
MluCI AATT 6 cut(s) 23, 67, 272, 320, 415, 557
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 6 cut(s) 70, 105, 376, 487, 600, 658
MseI TTAA 2 cut(s) 93, 612
MspA1I CMGCKG 3 cut(s) 197, 380, 502
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 81
MwoI GCNNNNNNNGC 2 cut(s) 149, 585
NciI CCSGG 1 cut(s) 81
NdeII GATC 3 cut(s) 385, 488, 607
NlaIII CATG 1 cut(s) 519
NmuCI GTSAC 1 cut(s) 199
PfeI GAWTC 4 cut(s) 131, 397, 506, 653
PkrI GCNGC 2 cut(s) 196, 581
PpuMI RGGWCCY 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 460
PspPI GGNCC 3 cut(s) 114, 460, 587
PspPPI RGGWCCY 1 cut(s) 460
PstI CTGCAG 1 cut(s) 374
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 2 cut(s) 488, 607
PvuII CAGCTG 2 cut(s) 380, 502
RsaI GTAC 2 cut(s) 177, 189
RsaNI GTAC 2 cut(s) 176, 188
SaqAI TTAA 2 cut(s) 93, 612
SatI GCNGC 2 cut(s) 195, 580
Sau3AI GATC 3 cut(s) 385, 488, 607
Sau96I GGNCC 3 cut(s) 114, 460, 587
ScrFI CCNGG 1 cut(s) 81
SfaNI GCATC 2 cut(s) 538, 591
SfcI CTRYAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 460
SmlI CTYRAG 2 cut(s) 267, 277
SmoI CTYRAG 2 cut(s) 267, 277
Sse9I AATT 6 cut(s) 23, 67, 272, 320, 415, 557
SsiI CCGC 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 79
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 1 cut(s) 485
TaiI ACGT 1 cut(s) 456
TaqI TCGA 1 cut(s) 16
TasI AATT 6 cut(s) 23, 67, 272, 320, 415, 557
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 4 cut(s) 131, 397, 506, 653
Tru1I TTAA 2 cut(s) 93, 612
Tru9I TTAA 2 cut(s) 93, 612
TscAI CASTG 2 cut(s) 208, 574
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 1 cut(s) 579
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 464, 504
TspRI CASTG 2 cut(s) 208, 574
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 4 cut(s) 67, 272, 320, 415
XcmI CCANNNNNNNNNTGG 2 cut(s) 169, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.