AT4G29670

Thioredoxin-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0009979
Physical Location & Seq
Reverse (-)
14535407 .. 14537356
1950 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G29670.2

Sequence Viewer

Length: 708 bp
ATGGCTGGAGTTGTGCGATTAACGACGACGTCGGTTCAAGCCATTCGTGTCTCCTCGTCCTTCAGCTCCTTTGCCACCGCTCTTAATCCTCTGCAACCTTGCTTACCTCCAAACTCGAATCTGAATTCCGACAAGAGATTGCGTCTTCTCTCATCTTCTCCTTCCTGCTCGTCTTCTCATTACCACCCATCTTCTGGTCTCGGAAGCCATCTCCCCCTTAGACGACCCAAGAGCCAAGTGGTTCGTGTCAAGGTAGATGAAAATGTAGCCGAGACAGAGCCACCGAAATGGTGGGAGAGGAATGCTCCAAACATGGTCGATATTCATTCTACTGAAGAGTTCTTGAGCGCTTTAAGCGGTGCGGGTGAAAGATTAGTTATTGTAGAGTTTTATGGAACTTGGTGTGCTTCTTGCAGAGCATTGTTCCCAAAGCTTTGCAAGACAGCGGTGGAACATCCCGATATAGTGTTCCTTAAAGTAAACTTTGATGAGAATAAACCGATGTGCAAGAGTTTGAACGTGAGAGTGCTTCCTTTTTTCCACTTTTACCGTGGAGCTGATGGTCAGCTTGAGTCATTCTCATGTTCTCTTGCTAAGTTTCAGAAGATAAAAGATGCAATCCAACTGCACAATACCGATCGTTGCAGCCTCGGTCCAGCCAAGGTACCTGAAGGCTTAACATTAGCCAAACCAGCTGCATCAAGTTGA

Protein Analysis

235

Amino Acids

25.84

Weight (kDa)

9.06

Isoelectric Point (pI)

45.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 108 - 191 8.3e-17 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 32
Acc65I GGTACC 1 cut(s) 664
AccB1I GGYRCC 1 cut(s) 664
AccB7I CCANNNNNTGG 1 cut(s) 194
AccBSI CCGCTC 1 cut(s) 80
AciI CCGC 4 cut(s) 78, 357, 362, 446
AcsI RAATTY 1 cut(s) 124
AcuI CTGAAG 3 cut(s) 46, 354, 690
AcyI GRCGYC 1 cut(s) 29
AfaI GTAC 1 cut(s) 666
AfeI AGCGCT 1 cut(s) 349
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 2 cut(s) 38, 517
AluBI AGCT 5 cut(s) 66, 433, 557, 568, 695
AluI AGCT 5 cut(s) 66, 433, 557, 568, 695
Alw26I GTCTC 3 cut(s) 55, 203, 266
Aor51HI AGCGCT 1 cut(s) 349
ApeKI GCWGC 2 cut(s) 645, 695
ApoI RAATTY 1 cut(s) 124
Asp718I GGTACC 1 cut(s) 664
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 1 cut(s) 653
AsuHPI GGTGA 1 cut(s) 377
AvaII GGWCC 1 cut(s) 653
BanI GGYRCC 1 cut(s) 664
BbsI GAAGAC 2 cut(s) 137, 165
BbvI GCAGC 2 cut(s) 657, 682
BccI CCATC 3 cut(s) 196, 216, 554
BcoDI GTCTC 3 cut(s) 55, 203, 266
BfoI RGCGCY 1 cut(s) 351
BisI GCNGC 2 cut(s) 646, 696
BlsI GCNGC 2 cut(s) 647, 697
Bme18I GGWCC 1 cut(s) 653
BmgT120I GGNCC 1 cut(s) 653
BmiI GGNNCC 1 cut(s) 666
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 2 cut(s) 137, 165
BplI GAGNNNNNCTC 4 cut(s) 289, 321, 563, 595
BpmI CTGGAG 1 cut(s) 27
BpuEI CTTGAG 2 cut(s) 364, 590
BsaHI GRCGYC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 203
BsaJI CCNNGG 3 cut(s) 550, 649, 660
Bsc4I CCNNNNNNNGG 1 cut(s) 194
BseDI CCNNGG 3 cut(s) 550, 649, 660
BseGI GGATG 1 cut(s) 454
BseLI CCNNNNNNNGG 1 cut(s) 194
BseRI GAGGAG 1 cut(s) 43
BseXI GCAGC 2 cut(s) 657, 682
BsgI GTGCAG 1 cut(s) 611
Bsh1285I CGRYCG 1 cut(s) 640
BshNI GGYRCC 1 cut(s) 664
BsiEI CGRYCG 1 cut(s) 640
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 3 cut(s) 55, 203, 266
BsmI GAATGC 1 cut(s) 307
Bso31I GGTCTC 1 cut(s) 203
Bsp143I GATC 1 cut(s) 637
BspACI CCGC 4 cut(s) 78, 357, 362, 446
BspLI GGNNCC 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 664
BspTNI GGTCTC 1 cut(s) 203
BsrBI CCGCTC 1 cut(s) 80
BssECI CCNNGG 3 cut(s) 550, 649, 660
BssMI GATC 1 cut(s) 637
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 1 cut(s) 660
Bst4CI ACNGT 1 cut(s) 551
Bst6I CTCTTC 1 cut(s) 330
BstACI GRCGYC 1 cut(s) 29
BstDEI CTNAG 2 cut(s) 218, 594
BstDSI CCRYGG 1 cut(s) 550
BstF5I GGATG 1 cut(s) 454
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 1 cut(s) 640
BstMAI GTCTC 3 cut(s) 55, 203, 266
BstMBI GATC 1 cut(s) 637
BstMCI CGRYCG 1 cut(s) 640
BstMWI GCNNNNNNNGC 2 cut(s) 354, 692
BstV1I GCAGC 2 cut(s) 657, 682
BstV2I GAAGAC 2 cut(s) 137, 165
BstXI CCANNNNNNTGG 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 550
BtsCI GGATG 1 cut(s) 454
CfoI GCGC 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 653
CseI GACGC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 665
CviAII CATG 2 cut(s) 313, 582
CviQI GTAC 1 cut(s) 665
DdeI CTNAG 2 cut(s) 218, 594
DpnI GATC 1 cut(s) 639
DpnII GATC 1 cut(s) 637
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Eco130I CCWWGG 1 cut(s) 660
Eco31I GGTCTC 1 cut(s) 203
Eco47I GGWCC 1 cut(s) 653
Eco47III AGCGCT 1 cut(s) 349
Eco57I CTGAAG 3 cut(s) 46, 354, 690
EcoRI GAATTC 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 660
ErhI CCWWGG 1 cut(s) 660
FaeI CATG 2 cut(s) 316, 585
FaiI YATR 4 cut(s) 314, 393, 464, 583
FatI CATG 2 cut(s) 312, 581
FauI CCCGC 1 cut(s) 355
Fnu4HI GCNGC 2 cut(s) 646, 696
FokI GGATG 1 cut(s) 441
Fsp4HI GCNGC 2 cut(s) 646, 696
GlaI GCGC 1 cut(s) 349
GluI GCNGC 2 cut(s) 646, 696
GsuI CTGGAG 1 cut(s) 27
HaeII RGCGCY 1 cut(s) 351
HgaI GACGC 1 cut(s) 131
HhaI GCGC 1 cut(s) 350
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 2 cut(s) 316, 585
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HindIII AAGCTT 1 cut(s) 431
HinfI GANTC 2 cut(s) 118, 572
HphI GGTGA 1 cut(s) 377
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 4 cut(s) 123, 130, 203, 603
Hpy188III TCNNGA 2 cut(s) 343, 458
Hpy8I GTNNAC 1 cut(s) 481
Hpy99I CGWCG 3 cut(s) 28, 31, 34
HpyAV CCTTC 3 cut(s) 70, 171, 665
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4IV ACGT 2 cut(s) 29, 519
HpyCH4V TGCA 8 cut(s) 94, 414, 438, 507, 617, 628, 645, 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 354, 692
HpyF3I CTNAG 2 cut(s) 218, 594
HpySE526I ACGT 2 cut(s) 29, 519
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 2 cut(s) 316, 585
HspAI GCGC 1 cut(s) 348
KpnI GGTACC 1 cut(s) 668
Kzo9I GATC 1 cut(s) 637
LmnI GCTCC 3 cut(s) 71, 310, 554
LpnPI CCDG 4 cut(s) 178, 180, 669, 681
Lsp1109I GCAGC 2 cut(s) 657, 682
LweI GCATC 1 cut(s) 604
MaeII ACGT 2 cut(s) 29, 519
MalI GATC 1 cut(s) 639
MbiI CCGCTC 1 cut(s) 80
MboI GATC 1 cut(s) 637
MboII GAAGA 6 cut(s) 137, 147, 165, 183, 347, 616
MluCI AATT 1 cut(s) 124
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 2 cut(s) 153, 646
MnlI CCTC 5 cut(s) 64, 99, 117, 291, 659
MseI TTAA 5 cut(s) 20, 84, 353, 474, 677
MslI CAYNNNNRTG 2 cut(s) 286, 580
MspA1I CMGCKG 2 cut(s) 446, 695
Mva1269I GAATGC 1 cut(s) 307
MwoI GCNNNNNNNGC 2 cut(s) 354, 692
NdeII GATC 1 cut(s) 637
NlaIII CATG 2 cut(s) 316, 585
NlaIV GGNNCC 1 cut(s) 666
NmeAIII GCCGAG 1 cut(s) 295
PctI GAATGC 1 cut(s) 307
PfeI GAWTC 1 cut(s) 118
PflFI GACNNNGTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 194
PkrI GCNGC 2 cut(s) 647, 697
Ple19I CGATCG 1 cut(s) 640
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
PspN4I GGNNCC 1 cut(s) 666
PspPI GGNCC 1 cut(s) 653
PsyI GACNNNGTC 1 cut(s) 28
PvuI CGATCG 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 1 cut(s) 666
RsaNI GTAC 1 cut(s) 665
RseI CAYNNNNRTG 2 cut(s) 286, 580
SaqAI TTAA 5 cut(s) 20, 84, 353, 474, 677
SatI GCNGC 2 cut(s) 646, 696
Sau3AI GATC 1 cut(s) 637
Sau96I GGNCC 1 cut(s) 653
SchI GAGTC 1 cut(s) 581
SfaNI GCATC 1 cut(s) 604
SinI GGWCC 1 cut(s) 653
SmiMI CAYNNNNRTG 2 cut(s) 286, 580
SmlI CTYRAG 2 cut(s) 343, 569
SmoI CTYRAG 2 cut(s) 343, 569
Sse9I AATT 1 cut(s) 124
SsiI CCGC 4 cut(s) 78, 357, 362, 446
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 1 cut(s) 551
TaiI ACGT 2 cut(s) 32, 522
TaqI TCGA 2 cut(s) 116, 318
TaqII GACCGA 1 cut(s) 641
TasI AATT 1 cut(s) 124
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 5 cut(s) 20, 84, 353, 474, 677
Tru9I TTAA 5 cut(s) 20, 84, 353, 474, 677
TseI GCWGC 2 cut(s) 645, 695
TspDTI ATGAA 2 cut(s) 273, 314
Tth111I GACNNNGTC 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 653
XapI RAATTY 1 cut(s) 124
XcmI CCANNNNNNNNNTGG 4 cut(s) 191, 235, 288, 548
ZraI GACGTC 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.