Rh2BG080400

Thioredoxin-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009979
Physical Location & Seq
Reverse (-)
6102419 .. 6105066
2648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG080400.1

Sequence Viewer

Length: 591 bp
ATGGCTGATGTTGTTCGATTACAATTCCATTCGCTTCGGTTCTCTTCGTCTCTGCTCACCTCTTTGAATTCTCTGCAACCGGGTCTCTTTGTTAACCAAAACCCATTTAGGAGGGCCTATGCTCTCTCTGATTCTTCCCTTGCTCGCTTTGCTTACAGACCCAGAAAGCAGTTGGTACATTTCAAGGTACAGGCCGCTGTCACTGAAACAGACCAGCCAAAATGGTGGGAAAGAAGTGTTCCGAATATGATTGACATACATTCTACTCAAGAATTCTTGAGTGCTTTAGGTCAAGCCGGAGATAGATTAGTTATTGTAGAATTCTACGGCACTTGGTGTGGTTCTTGCCGTGCATTATTTCCTAAGCTCTGCAGAACAGCTGAGGATCACCCTGAGATTCTATTCCTGAAAGTGAATTTTGATGAGAATAAGCCAATGTGCAAGAGTATGAACGTGAAGGTCCTTCCCTATTTCCACTTTTACCGTGGATCTGAGGGACAGCTGGAATCCTTTTCATGTTCACTTGCTAAGGTAATTCTAAAGTTTCATTCTTTTATCAATCATGTTACAGAGAGCTTCCTTCTGTCCTAA

Protein Analysis

196

Amino Acids

22.55

Weight (kDa)

9.02

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 87 - 170 4.4e-18 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 195
AclWI GGATC 2 cut(s) 393, 496
AcsI RAATTY 4 cut(s) 67, 272, 320, 415
AdeI CACNNNGTG 1 cut(s) 336
AfaI GTAC 2 cut(s) 177, 189
AgsI TTSAA 2 cut(s) 67, 184
AluBI AGCT 4 cut(s) 367, 380, 502, 576
AluI AGCT 4 cut(s) 367, 380, 502, 576
Alw26I GTCTC 2 cut(s) 54, 89
AlwI GGATC 2 cut(s) 393, 496
AoxI GGCC 2 cut(s) 114, 192
ApoI RAATTY 4 cut(s) 67, 272, 320, 415
AspS9I GGNCC 2 cut(s) 114, 460
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 49, 380
AvaII GGWCC 1 cut(s) 460
BbvCI CCTCAGC 1 cut(s) 381
BceAI ACGGC 2 cut(s) 333, 343
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 54, 89
BfmI CTRYAG 1 cut(s) 370
BisI GCNGC 1 cut(s) 195
BlsI GCNGC 1 cut(s) 196
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 2 cut(s) 114, 460
BmrFI CCNGG 1 cut(s) 81
Bpu10I CCTNAGC 3 cut(s) 363, 381, 528
BpuEI CTTGAG 2 cut(s) 252, 298
BpuMI CCSGG 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 484
BseDI CCNNGG 1 cut(s) 484
BseMII CTCAG 3 cut(s) 372, 384, 483
BshFI GGCC 2 cut(s) 116, 194
BsiSI CCGG 2 cut(s) 80, 297
BslFI GGGAC 1 cut(s) 510
BsmAI GTCTC 2 cut(s) 54, 89
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 1 cut(s) 510
BsnI GGCC 2 cut(s) 116, 194
Bso31I GGTCTC 1 cut(s) 89
Bsp143I GATC 2 cut(s) 385, 488
BspACI CCGC 1 cut(s) 195
BspANI GGCC 2 cut(s) 116, 194
BspCNI CTCAG 3 cut(s) 373, 385, 484
BspMAI CTGCAG 1 cut(s) 374
BspPI GGATC 2 cut(s) 393, 496
BspTNI GGTCTC 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 484
BssMI GATC 2 cut(s) 385, 488
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 1 cut(s) 49
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 5 cut(s) 363, 381, 393, 492, 528
BstDSI CCRYGG 1 cut(s) 484
BstKTI GATC 2 cut(s) 388, 491
BstMAI GTCTC 2 cut(s) 54, 89
BstMBI GATC 2 cut(s) 385, 488
BstMWI GCNNNNNNNGC 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 370
BstX2I RGATCY 1 cut(s) 488
BstXI CCANNNNNNTGG 1 cut(s) 225
BstYI RGATCY 1 cut(s) 488
BsuRI GGCC 2 cut(s) 116, 194
BtgI CCRYGG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 201
Cac8I GCNNGC 1 cut(s) 145
Cfr13I GGNCC 2 cut(s) 114, 460
Csp6I GTAC 2 cut(s) 176, 188
CviAII CATG 2 cut(s) 516, 563
CviQI GTAC 2 cut(s) 176, 188
DdeI CTNAG 5 cut(s) 363, 381, 393, 492, 528
DpnI GATC 2 cut(s) 387, 490
DpnII GATC 2 cut(s) 385, 488
DraIII CACNNNGTG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 460
EcoO109I RGGNCCY 2 cut(s) 114, 460
EcoRI GAATTC 3 cut(s) 67, 272, 320
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 2 cut(s) 519, 566
FaiI YATR 6 cut(s) 120, 248, 257, 449, 517, 564
FaqI GGGAC 1 cut(s) 510
FatI CATG 2 cut(s) 515, 562
Fnu4HI GCNGC 1 cut(s) 195
Fsp4HI GCNGC 1 cut(s) 195
GluI GCNGC 1 cut(s) 195
HaeIII GGCC 2 cut(s) 116, 194
HapII CCGG 2 cut(s) 80, 297
Hin1II CATG 2 cut(s) 519, 566
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HinfI GANTC 3 cut(s) 131, 397, 506
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 2 cut(s) 80, 297
HphI GGTGA 2 cut(s) 49, 380
Hpy166II GTNNAC 2 cut(s) 94, 521
Hpy188I TCNGA 3 cut(s) 130, 243, 493
Hpy188III TCNNGA 3 cut(s) 269, 277, 406
Hpy8I GTNNAC 2 cut(s) 94, 521
HpyAV CCTTC 3 cut(s) 451, 473, 590
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 4 cut(s) 76, 353, 372, 441
HpyF10VI GCNNNNNNNGC 1 cut(s) 149
HpyF3I CTNAG 5 cut(s) 363, 381, 393, 492, 528
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 2 cut(s) 519, 566
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 2 cut(s) 385, 488
LpnPI CCDG 8 cut(s) 93, 175, 176, 227, 310, 405, 419, 488
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 2 cut(s) 199, 565
MalI GATC 2 cut(s) 387, 490
MboI GATC 2 cut(s) 385, 488
MboII GAAGA 2 cut(s) 36, 126
MflI RGATCY 1 cut(s) 488
MluCI AATT 6 cut(s) 23, 67, 272, 320, 415, 534
MnlI CCTC 4 cut(s) 70, 105, 376, 487
MseI TTAA 1 cut(s) 93
MspA1I CMGCKG 3 cut(s) 197, 380, 502
MspI CCGG 2 cut(s) 80, 297
MspR9I CCNGG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 149
NciI CCSGG 1 cut(s) 81
NdeII GATC 2 cut(s) 385, 488
NlaIII CATG 2 cut(s) 519, 566
NmuCI GTSAC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 131, 397, 506
PkrI GCNGC 1 cut(s) 196
PpuMI RGGWCCY 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 460
PspPI GGNCC 2 cut(s) 114, 460
PspPPI RGGWCCY 1 cut(s) 460
PstI CTGCAG 1 cut(s) 374
PsuI RGATCY 1 cut(s) 488
PvuII CAGCTG 2 cut(s) 380, 502
RsaI GTAC 2 cut(s) 177, 189
RsaNI GTAC 2 cut(s) 176, 188
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 1 cut(s) 195
Sau3AI GATC 2 cut(s) 385, 488
Sau96I GGNCC 2 cut(s) 114, 460
ScrFI CCNGG 1 cut(s) 81
SfcI CTRYAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 460
SmlI CTYRAG 2 cut(s) 267, 277
SmoI CTYRAG 2 cut(s) 267, 277
Sse9I AATT 6 cut(s) 23, 67, 272, 320, 415, 534
SsiI CCGC 1 cut(s) 195
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 1 cut(s) 485
TaiI ACGT 1 cut(s) 456
TaqI TCGA 1 cut(s) 16
TasI AATT 6 cut(s) 23, 67, 272, 320, 415, 534
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 3 cut(s) 131, 397, 506
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 1 cut(s) 208
TseFI GTSAC 1 cut(s) 199
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 3 cut(s) 464, 504, 536
TspRI CASTG 1 cut(s) 208
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 4 cut(s) 67, 272, 320, 415
XcmI CCANNNNNNNNNTGG 2 cut(s) 169, 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.