MD02G1077400.v1.1

Thioredoxin-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009979
Physical Location & Seq
Reverse (-)
6093640 .. 6096500
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1077400.v1.1.491

Sequence Viewer

Length: 687 bp
ATGGCTGATGTCCTGAGATTACCATTCCATTCACTTCGTTTCTCTTCGTCTCTGCTCACCTCCTTCGCCTCCTCCGTAAGTTCTCTGCAACCGGGCCTCCCAGCCAATCAAAACCCATACAGGAGGGTTTATCCCCTTTCGTATTCGTCTGTTGCTCACTTCGCTTTCAGACCCAGAAAGCAGCTGGTTAATATCAAGGTACAGGCAACTGTTGCCGAAAGAGACAAGGACCAGCCAAAATGGTGGGAAAGAAGTGTTCCGAATATGGTCGACATTCATTCTACTCAAGAGCTTTTGAGTGCTTTAGGCCAAGCTGGTGATAGATTAGTTATTGTAGAATTCTACGGGACTTGGTGTGCTTCCTGCCGTGCATTGTTTCCTAAGCTCTGCAGAACAGCTGAAGACCACCCTGAGATTTTATTCCTGAAAGTGAATTTTGACGAGAACAAACCAATGTGCAAAAGTATGAACGTGAAGGTGCTCCCGTATTTCCACTTCTATCGTGGATCAGAGGGACAGCTTGAATCCTTTTCATGTTCACTTGCTAAGTTCCAGAAGTTAAAGGATGCTATTGCATTACACAACACAGCTCGTTGCAGCATTGGTCCTCCGAAGGGAGTTGGAGATCTTACACTGGAACCATCCTCAGCTCCGAAGGACAAGCCATCGGAATCTGCCTCGTCATAG

Protein Analysis

229

Amino Acids

25.5

Weight (kDa)

9.14

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 106 - 179 2.6e-16 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 270
AclWI GGATC 1 cut(s) 514
AcsI RAATTY 2 cut(s) 338, 433
AcuI CTGAAG 1 cut(s) 420
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 1 cut(s) 614
AgsI TTSAA 1 cut(s) 524
AluBI AGCT 8 cut(s) 184, 292, 314, 385, 398, 520, 590, 650
AluI AGCT 8 cut(s) 184, 292, 314, 385, 398, 520, 590, 650
Alw21I GWGCWC 1 cut(s) 483
Alw26I GTCTC 2 cut(s) 54, 216
AlwI GGATC 1 cut(s) 514
AoxI GGCC 2 cut(s) 94, 307
ApeKI GCWGC 2 cut(s) 181, 597
ApoI RAATTY 2 cut(s) 338, 433
AspS9I GGNCC 3 cut(s) 94, 229, 605
AsuC2I CCSGG 1 cut(s) 93
AsuHPI GGTGA 2 cut(s) 49, 329
AvaII GGWCC 2 cut(s) 229, 605
BarI GAAGNNNNNNTAC 2 cut(s) 479, 511
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 483
BbvCI CCTCAGC 1 cut(s) 646
BbvI GCAGC 2 cut(s) 193, 609
BccI CCATC 2 cut(s) 649, 673
BceAI ACGGC 1 cut(s) 351
BcnI CCSGG 1 cut(s) 93
BcoDI GTCTC 2 cut(s) 54, 216
BfmI CTRYAG 1 cut(s) 388
BglII AGATCT 1 cut(s) 625
BisI GCNGC 2 cut(s) 182, 598
BlsI GCNGC 2 cut(s) 183, 599
Bme1390I CCNGG 1 cut(s) 93
Bme18I GGWCC 2 cut(s) 229, 605
BmgT120I GGNCC 3 cut(s) 94, 229, 605
BmiI GGNNCC 1 cut(s) 639
BmrFI CCNGG 1 cut(s) 93
BmsI GCATC 1 cut(s) 556
BpiI GAAGAC 1 cut(s) 408
Bpu10I CCTNAGC 2 cut(s) 381, 646
BpuEI CTTGAG 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 93
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 1 cut(s) 614
Bse1I ACTGG 1 cut(s) 639
BseGI GGATG 2 cut(s) 571, 641
BseLI CCNNNNNNNGG 1 cut(s) 614
BseMII CTCAG 3 cut(s) 5, 402, 660
BseNI ACTGG 1 cut(s) 639
BseRI GAGGAG 1 cut(s) 61
BseXI GCAGC 2 cut(s) 193, 609
BseYI CCCAGC 1 cut(s) 100
BshFI GGCC 2 cut(s) 96, 309
BsiHKAI GWGCWC 1 cut(s) 483
BsiSI CCGG 1 cut(s) 92
BslFI GGGAC 2 cut(s) 361, 528
BslI CCNNNNNNNGG 1 cut(s) 614
BsmAI GTCTC 2 cut(s) 54, 216
BsmBI CGTCTC 1 cut(s) 54
BsmFI GGGAC 2 cut(s) 361, 528
BsnI GGCC 2 cut(s) 96, 309
Bsp1286I GDGCHC 1 cut(s) 483
Bsp143I GATC 2 cut(s) 506, 625
BspANI GGCC 2 cut(s) 96, 309
BspCNI CTCAG 3 cut(s) 6, 403, 659
BspLI GGNNCC 1 cut(s) 639
BspMAI CTGCAG 1 cut(s) 392
BspPI GGATC 1 cut(s) 514
BsrI ACTGG 1 cut(s) 639
BssMI GATC 2 cut(s) 506, 625
Bst4CI ACNGT 1 cut(s) 211
Bst6I CTCTTC 1 cut(s) 49
BstAPI GCANNNNNTGC 1 cut(s) 212
BstDEI CTNAG 5 cut(s) 14, 381, 411, 546, 646
BstF5I GGATG 2 cut(s) 571, 641
BstKTI GATC 2 cut(s) 509, 628
BstMAI GTCTC 2 cut(s) 54, 216
BstMBI GATC 2 cut(s) 506, 625
BstMWI GCNNNNNNNGC 2 cut(s) 161, 212
BstSCI CCNGG 1 cut(s) 91
BstSFI CTRYAG 1 cut(s) 388
BstV1I GCAGC 2 cut(s) 193, 609
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 625
BstXI CCANNNNNNTGG 1 cut(s) 243
BstYI RGATCY 1 cut(s) 625
BsuRI GGCC 2 cut(s) 96, 309
BtsCI GGATG 2 cut(s) 571, 641
BtsIMutI CAGTG 1 cut(s) 632
Cfr13I GGNCC 3 cut(s) 94, 229, 605
Csp6I GTAC 1 cut(s) 200
CviAII CATG 1 cut(s) 534
CviQI GTAC 1 cut(s) 200
DdeI CTNAG 5 cut(s) 14, 381, 411, 546, 646
DpnI GATC 2 cut(s) 508, 627
DpnII GATC 2 cut(s) 506, 625
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco47I GGWCC 2 cut(s) 229, 605
Eco57I CTGAAG 1 cut(s) 420
EcoRI GAATTC 1 cut(s) 338
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 1 cut(s) 537
FaiI YATR 5 cut(s) 118, 266, 467, 535, 685
FaqI GGGAC 2 cut(s) 361, 528
FatI CATG 1 cut(s) 533
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 182, 598
FokI GGATG 2 cut(s) 578, 628
Fsp4HI GCNGC 2 cut(s) 182, 598
GluI GCNGC 2 cut(s) 182, 598
GsaI CCCAGC 1 cut(s) 104
HaeIII GGCC 2 cut(s) 96, 309
HapII CCGG 1 cut(s) 92
Hin1II CATG 1 cut(s) 537
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 2 cut(s) 524, 671
HpaII CCGG 1 cut(s) 92
HphI GGTGA 2 cut(s) 49, 329
Hpy166II GTNNAC 2 cut(s) 271, 539
Hpy188I TCNGA 6 cut(s) 170, 261, 511, 612, 654, 670
Hpy188III TCNNGA 4 cut(s) 13, 287, 424, 553
Hpy8I GTNNAC 2 cut(s) 271, 539
HpyAV CCTTC 4 cut(s) 73, 469, 607, 649
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4IV ACGT 1 cut(s) 471
HpyCH4V TGCA 6 cut(s) 88, 371, 390, 459, 575, 597
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 212
HpyF3I CTNAG 5 cut(s) 14, 381, 411, 546, 646
HpySE526I ACGT 1 cut(s) 471
Hsp92II CATG 1 cut(s) 537
Kzo9I GATC 2 cut(s) 506, 625
LmnI GCTCC 2 cut(s) 486, 655
Lsp1109I GCAGC 2 cut(s) 193, 609
LweI GCATC 1 cut(s) 556
MaeII ACGT 1 cut(s) 471
MalI GATC 2 cut(s) 508, 627
MboI GATC 2 cut(s) 506, 625
MboII GAAGA 2 cut(s) 36, 413
MflI RGATCY 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 483
MluCI AATT 2 cut(s) 338, 433
MmeI TCCRAC 1 cut(s) 601
MnlI CCTC 8 cut(s) 70, 79, 82, 107, 117, 505, 618, 655
MseI TTAA 2 cut(s) 189, 560
MspA1I CMGCKG 2 cut(s) 184, 398
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 93
MwoI GCNNNNNNNGC 2 cut(s) 161, 212
NciI CCSGG 1 cut(s) 93
NdeII GATC 2 cut(s) 506, 625
NlaIII CATG 1 cut(s) 537
NlaIV GGNNCC 1 cut(s) 639
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 2 cut(s) 524, 671
PkrI GCNGC 2 cut(s) 183, 599
PspFI CCCAGC 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 639
PspPI GGNCC 3 cut(s) 94, 229, 605
PstI CTGCAG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 625
PvuII CAGCTG 2 cut(s) 184, 398
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SalI GTCGAC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 189, 560
SatI GCNGC 2 cut(s) 182, 598
Sau3AI GATC 2 cut(s) 506, 625
Sau96I GGNCC 3 cut(s) 94, 229, 605
ScrFI CCNGG 1 cut(s) 93
SduI GDGCHC 1 cut(s) 483
SfaNI GCATC 1 cut(s) 556
SfcI CTRYAG 1 cut(s) 388
SinI GGWCC 2 cut(s) 229, 605
SmlI CTYRAG 1 cut(s) 285
SmoI CTYRAG 1 cut(s) 285
Sse9I AATT 2 cut(s) 338, 433
StyD4I CCNGG 1 cut(s) 91
TaaI ACNGT 1 cut(s) 211
TaiI ACGT 1 cut(s) 474
TaqI TCGA 1 cut(s) 270
TasI AATT 2 cut(s) 338, 433
TfiI GAWTC 2 cut(s) 524, 671
Tru1I TTAA 2 cut(s) 189, 560
Tru9I TTAA 2 cut(s) 189, 560
TscAI CASTG 1 cut(s) 639
TseI GCWGC 2 cut(s) 181, 597
TspDTI ATGAA 3 cut(s) 266, 482, 522
TspGWI ACGGA 1 cut(s) 64
TspRI CASTG 1 cut(s) 639
VpaK11BI GGWCC 2 cut(s) 229, 605
XapI RAATTY 2 cut(s) 338, 433
XcmI CCANNNNNNNNNTGG 2 cut(s) 181, 500
XmiI GTMKAC 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.