FvH4_1g07270

Thioredoxin-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009979
Physical Location & Seq
Reverse (-)
3839250 .. 3841617
2368 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g07270.t1

Sequence Viewer

Length: 669 bp
ATGGCTGATGTTGTTCAATTACAATTCCATTCGCTTCGGTTCTCTTCGTCTCTGCTCACCTCTTTGAATTCTCTGCAACCGGGTCTCTTTGTTAATCAAAACCCATTTAGGAGGGCCTATGCTCTCTCTGATAAGAACCTTGCTCGCTTTGCTTACAGACCCAGAAAGCAGTTGGTGCATTTCAAGGTACATGCCGCTGTTGCTGAAACAGACCAGCCAAAATGGTGGGAAAGAAGTGTTCCAAATATGATTGACATACATTCTACTCAAGAATTCTTGAGTGCTTTAGGTCAAGCTGGAGATAGATTAGTTATTGTAGAATTCTATGGCACTTGGTGTGCTTCTTGCCGTGCATTATTTCCTAAGCTCTGCAGAACAGCCGAGGATCACCCTGAGATTTTATTCCTGAAAGTGAATTTTGATGAGAATAAGCCAATGTGCAAGAGTATGAACGTGAAGGTCCTTCCCTATTTCCACTTTTACCGTGGATCTGAAGGAAAACTGGAGTCCTTTTCGTGTTCACTAGCTAAGTTCCAGAAAATAAAGGATGCTATCCAATTACACAACACTGCTCGTTGCAGCATCGGCCCTCCACAGGGTGTTGGAGATCTGAAACTGGAACCTTCCTTGGTTCAGAAGGACACACCCTCAGAATCTGCTTCAGCATAG

Protein Analysis

223

Amino Acids

25.12

Weight (kDa)

9.03

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 87 - 174 3.4e-17 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 195
AclWI GGATC 2 cut(s) 393, 496
AcsI RAATTY 4 cut(s) 67, 272, 320, 415
AcuI CTGAAG 2 cut(s) 513, 645
AdeI CACNNNGTG 2 cut(s) 336, 599
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 2 cut(s) 595, 596
AgsI TTSAA 3 cut(s) 17, 67, 184
AluBI AGCT 3 cut(s) 296, 367, 527
AluI AGCT 3 cut(s) 296, 367, 527
Alw26I GTCTC 2 cut(s) 54, 89
AlwI GGATC 2 cut(s) 393, 496
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 2 cut(s) 114, 586
ApeKI GCWGC 1 cut(s) 579
ApoI RAATTY 4 cut(s) 67, 272, 320, 415
AspS9I GGNCC 3 cut(s) 114, 460, 587
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 49, 380
AvaII GGWCC 1 cut(s) 460
BbvI GCAGC 1 cut(s) 591
BceAI ACGGC 1 cut(s) 333
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 54, 89
BfaI CTAG 1 cut(s) 524
BfmI CTRYAG 1 cut(s) 370
BglII AGATCT 1 cut(s) 607
BisI GCNGC 2 cut(s) 195, 580
BlsI GCNGC 2 cut(s) 196, 581
Bme1390I CCNGG 1 cut(s) 81
Bme18I GGWCC 1 cut(s) 460
BmgT120I GGNCC 3 cut(s) 114, 460, 587
BmiI GGNNCC 1 cut(s) 621
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 538, 591
BpmI CTGGAG 2 cut(s) 318, 524
Bpu10I CCTNAGC 1 cut(s) 363
BpuEI CTTGAG 2 cut(s) 252, 298
BpuMI CCSGG 1 cut(s) 81
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 3 cut(s) 381, 484, 627
Bsc4I CCNNNNNNNGG 2 cut(s) 595, 596
Bse1I ACTGG 2 cut(s) 507, 621
BseDI CCNNGG 3 cut(s) 381, 484, 627
BseGI GGATG 1 cut(s) 553
BseLI CCNNNNNNNGG 2 cut(s) 595, 596
BseMII CTCAG 2 cut(s) 384, 663
BseNI ACTGG 2 cut(s) 507, 621
BseXI GCAGC 1 cut(s) 591
BshFI GGCC 2 cut(s) 116, 588
BsiSI CCGG 1 cut(s) 80
BslI CCNNNNNNNGG 2 cut(s) 595, 596
BsmAI GTCTC 2 cut(s) 54, 89
BsmBI CGTCTC 1 cut(s) 54
BsnI GGCC 2 cut(s) 116, 588
Bso31I GGTCTC 1 cut(s) 89
Bsp143I GATC 3 cut(s) 385, 488, 607
BspACI CCGC 1 cut(s) 195
BspANI GGCC 2 cut(s) 116, 588
BspCNI CTCAG 2 cut(s) 385, 662
BspLI GGNNCC 1 cut(s) 621
BspMAI CTGCAG 1 cut(s) 374
BspPI GGATC 2 cut(s) 393, 496
BspTNI GGTCTC 1 cut(s) 89
BsrI ACTGG 2 cut(s) 507, 621
BssECI CCNNGG 3 cut(s) 381, 484, 627
BssMI GATC 3 cut(s) 385, 488, 607
BssT1I CCWWGG 1 cut(s) 627
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 1 cut(s) 49
BstAPI GCANNNNNTGC 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 145
BstDEI CTNAG 4 cut(s) 363, 393, 528, 649
BstDSI CCRYGG 1 cut(s) 484
BstF5I GGATG 1 cut(s) 553
BstKTI GATC 3 cut(s) 388, 491, 610
BstMAI GTCTC 2 cut(s) 54, 89
BstMBI GATC 3 cut(s) 385, 488, 607
BstMWI GCNNNNNNNGC 4 cut(s) 149, 175, 200, 585
BstNSI RCATGY 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 370
BstV1I GCAGC 1 cut(s) 591
BstX2I RGATCY 2 cut(s) 488, 607
BstXI CCANNNNNNTGG 1 cut(s) 225
BstYI RGATCY 2 cut(s) 488, 607
BsuRI GGCC 2 cut(s) 116, 588
BtgI CCRYGG 1 cut(s) 484
BtsCI GGATG 1 cut(s) 553
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 1 cut(s) 567
Cac8I GCNNGC 1 cut(s) 145
CaiI CAGNNNCTG 1 cut(s) 656
Cfr13I GGNCC 3 cut(s) 114, 460, 587
Csp6I GTAC 1 cut(s) 188
CviAII CATG 1 cut(s) 191
CviJI RGCY 9 cut(s) 5, 116, 217, 296, 367, 380, 433, 527, 588
CviKI_1 RGCY 9 cut(s) 5, 116, 217, 296, 367, 380, 433, 527, 588
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 4 cut(s) 363, 393, 528, 649
DpnI GATC 3 cut(s) 387, 490, 609
DpnII GATC 3 cut(s) 385, 488, 607
DraIII CACNNNGTG 2 cut(s) 336, 599
Eam1104I CTCTTC 1 cut(s) 49
EarI CTCTTC 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 627
Eco31I GGTCTC 1 cut(s) 89
Eco47I GGWCC 1 cut(s) 460
Eco57I CTGAAG 2 cut(s) 513, 645
EcoO109I RGGNCCY 2 cut(s) 114, 460
EcoRI GAATTC 3 cut(s) 67, 272, 320
EcoT14I CCWWGG 1 cut(s) 627
ErhI CCWWGG 1 cut(s) 627
Esp3I CGTCTC 1 cut(s) 54
FaeI CATG 1 cut(s) 194
FaiI YATR 7 cut(s) 120, 192, 248, 257, 327, 449, 667
FatI CATG 1 cut(s) 190
Fnu4HI GCNGC 2 cut(s) 195, 580
FokI GGATG 1 cut(s) 560
Fsp4HI GCNGC 2 cut(s) 195, 580
FspBI CTAG 1 cut(s) 524
GluI GCNGC 2 cut(s) 195, 580
GsuI CTGGAG 2 cut(s) 318, 524
HaeIII GGCC 2 cut(s) 116, 588
HapII CCGG 1 cut(s) 80
Hin1II CATG 1 cut(s) 194
HinfI GANTC 2 cut(s) 506, 653
HpaII CCGG 1 cut(s) 80
HphI GGTGA 2 cut(s) 49, 380
Hpy166II GTNNAC 1 cut(s) 521
Hpy188I TCNGA 5 cut(s) 130, 493, 612, 636, 652
Hpy188III TCNNGA 4 cut(s) 269, 277, 406, 535
Hpy8I GTNNAC 1 cut(s) 521
HpyAV CCTTC 5 cut(s) 451, 473, 488, 631, 633
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 6 cut(s) 76, 178, 353, 372, 441, 579
HpyF10VI GCNNNNNNNGC 4 cut(s) 149, 175, 200, 585
HpyF3I CTNAG 4 cut(s) 363, 393, 528, 649
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 1 cut(s) 194
Kzo9I GATC 3 cut(s) 385, 488, 607
Lsp1109I GCAGC 1 cut(s) 591
LweI GCATC 2 cut(s) 538, 591
MaeI CTAG 1 cut(s) 524
MaeII ACGT 1 cut(s) 453
MalI GATC 3 cut(s) 387, 490, 609
MboI GATC 3 cut(s) 385, 488, 607
MboII GAAGA 1 cut(s) 36
MflI RGATCY 2 cut(s) 488, 607
MluCI AATT 7 cut(s) 17, 23, 67, 272, 320, 415, 557
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 1 cut(s) 583
MnlI CCTC 5 cut(s) 70, 105, 376, 600, 658
MseI TTAA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 81
MwoI GCNNNNNNNGC 4 cut(s) 149, 175, 200, 585
NciI CCSGG 1 cut(s) 81
NdeII GATC 3 cut(s) 385, 488, 607
NlaIII CATG 1 cut(s) 194
NlaIV GGNNCC 1 cut(s) 621
NmeAIII GCCGAG 1 cut(s) 406
NspI RCATGY 1 cut(s) 194
PfeI GAWTC 1 cut(s) 653
PkrI GCNGC 2 cut(s) 196, 581
PleI GAGTC 1 cut(s) 514
PpsI GAGTC 1 cut(s) 514
PpuMI RGGWCCY 1 cut(s) 460
Psp5II RGGWCCY 1 cut(s) 460
PspN4I GGNNCC 1 cut(s) 621
PspPI GGNCC 3 cut(s) 114, 460, 587
PspPPI RGGWCCY 1 cut(s) 460
PstI CTGCAG 1 cut(s) 374
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 2 cut(s) 488, 607
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 2 cut(s) 195, 580
Sau3AI GATC 3 cut(s) 385, 488, 607
Sau96I GGNCC 3 cut(s) 114, 460, 587
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 1 cut(s) 81
SfaNI GCATC 2 cut(s) 538, 591
SfcI CTRYAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 460
SmlI CTYRAG 2 cut(s) 267, 277
SmoI CTYRAG 2 cut(s) 267, 277
Sse9I AATT 7 cut(s) 17, 23, 67, 272, 320, 415, 557
SsiI CCGC 1 cut(s) 195
SspMI CTAG 1 cut(s) 524
StyD4I CCNGG 1 cut(s) 79
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 1 cut(s) 485
TaiI ACGT 1 cut(s) 456
TasI AATT 7 cut(s) 17, 23, 67, 272, 320, 415, 557
TauI GCSGC 1 cut(s) 197
TfiI GAWTC 1 cut(s) 653
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TscAI CASTG 1 cut(s) 574
TseI GCWGC 1 cut(s) 579
TspDTI ATGAA 1 cut(s) 464
TspRI CASTG 1 cut(s) 574
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 4 cut(s) 67, 272, 320, 415
XceI RCATGY 1 cut(s) 194
XcmI CCANNNNNNNNNTGG 2 cut(s) 169, 482
XspI CTAG 1 cut(s) 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.