AT4G27220

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011026
Physical Location & Seq
Reverse (-)
13633941 .. 13637119
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27220.1

Sequence Viewer

Length: 2760 bp
ATGTTCAGATCGAACGCAAGAGCGTTGAATAGAGCATTGGAGAGACTAAAGAATGTGCAGACCAAGGTGAATGAAGCTCTTAAGAGATCAGGGATCCAAGAGAAATCTTTGGAGCGAAAGCTGAGGATATGGCTAAGAAAGGTCGAAGAGAATGTGCCTCTTGGTGAGCTGATTTTGGAGAAACGCTCTTCATGTGCTATATGGCTGAGCGACAAAGATGTGGAAATTCTTGAGAAAGTCAAAAGGCTGGAGGAGCAAGGCCAAGATCTCATAAAAAAGATTTCAGTCAACAAGTCATCTAGAGAAATAGTTGAACGGGTCTTGGGGCCTTCATTTCATCCTCAAAAAACAGCACTGGAGATGTTGGATAAGCTAAAGGATTGTTTGAAGAAGAAGAATGTTCAAAAGATTGGTGTTTGGGGTATGGGCGGTGTTGGGAAGACAACACTTGTCAGGACGCTTAACAATGATCTCTTGAAATATGCTGCTACTCAACAGTTTGCTTTGGTAATTTGGGTGACAGTGTCGAAAGATTTTGACTTAAAAAGGGTCCAGATGGACATAGCCAAGAGACTGGGCAAGCGATTTACAAGGGAGCAAATGAATCAGCTAGGCTTAACTATTTGTGAGAGGCTAATAGATTTGAAAAATTTCCTCCTTATTCTTGACGATGTTTGGCACCCCATTGATTTGGACCAATTGGGTATTCCATTAGCATTGGAACGATCGAAGGATTCAAAGGTTGTTCTAACTTCAAGAAGACTAGAAGTGTGCCAACAGATGATGACTAATGAAAACATCAAAGTTGCATGCTTACAAGAGAAAGAAGCTTGGGAATTGTTTTGCCACAACGTTGGAGAAGTAGCAAACTCTGATAATGTTAAGCCCATTGCAAAGGATGTATCCCATGAATGTTGTGGATTGCCTTTGGCTATCATCACAATCGGGCGGACCCTGCGCGGAAAACCTCAAGTCGAGGTATGGAAACACACCTTGAATTTGCTAAAGAGATCAGCACCTTCTATCGATACTGAAGAAAAGATCTTTGGTACTTTAAAACTCAGCTACGACTTTCTTCAAGACAATATGAAAAGTTGCTTCCTTTTCTGTGCTTTATTTCCGGAGGACTACTCTATCAAAGTAAGTGAACTAATAATGTACTGGGTCGCGGAAGGATTGTTGGATGGACAACATCATTACGAGGACATGATGAATGAGGGAGTTACTTTGGTTGAGAGATTGAAGGACTCTTGCCTGTTAGAAGATGGTGATAGTTGTGATACAGTTAAAATGCACGATGTTGTGCGTGACTTTGCTATATGGTTCATGTCTTCTCAGGGTGAAGGATTTCATTCTCTTGTCATGGCCGGGAGAGGTCTAATAGAGTTTCCACAAGATAAGTTTGTTTCTTCAGTCCAGAGAGTTTCTTTGATGGCTAATAAGCTAGAAAGACTACCTAATAATGTGATAGAGGGTGTGGAAACATTAGTTTTACTACTACAAGGGAATTCCCATGTTAAGGAAGTTCCTAATGGGTTCTTACAAGCATTCCCAAACCTTAGAATCTTGGATCTCAGTGGTGTTCGTATAAGAACATTGCCAGACTCCTTTTCAAATCTTCATAGTTTGCGGTCTCTTGTGTTAAGAAACTGTAAAAAATTGAGGAACCTGCCTTCGCTAGAATCCCTTGTTAAACTCCAATTTCTTGATCTACATGAATCTGCCATTAGGGAACTTCCGAGAGGTCTTGAAGCGTTGAGCAGCTTAAGATACATTTGCGTTTCCAACACATATCAACTCCAGAGCATTCCGGCTGGAACTATTCTCCAGTTATCAAGTTTGGAGGTTCTAGACATGGCGGGGAGTGCTTATAGTTGGGGCATCAAGGGAGAAGAAAGAGAAGGACAAGCAACACTTGACGAGGTTACATGCCTCCCTCATTTGCAGTTTCTTGCCATTAAGCTCCTCGATGTCTTGTCCTTTTCCTATGAATTTGATTCTTTGACCAAAAGGCTAACAAAGTTCCAGTTTCTATTTTCACCTATTCGATCGGTTTCACCTCCTGGCACCGGTGAAGGTTGCCTAGCAATCAGTGATGTCAATGTCTCAAATGCATCTATTGGGTGGCTACTGCAACATGTGACATCACTAGACTTGAACTATTGTGAGGGTCTTAATGGTATGTTCGAGAACTTGGTAACGAAGAGCAAAAGTAGCTTTGTTGCCATGAAAGCACTGTCCATTCACTATTTTCCCAGCTTAAGCCTTGCGAGTGGTTGTGAGTCCCAACTGGATCTCTTTCCAAATCTCGAGGAACTTTCTTTGGACAATGTCAATCTGGAAAGCATCGGAGAACTTAATGGTTTCCTCGGGATGAGACTTCAGAAACTAAAACTACTTCAAGTCTCTGGCTGCCGACAGCTTAAACGTCTTTTCTCAGACCAAATCTTAGCAGGTACTCTGCCAAACCTACAAGAGATAAAGGTTGTCTCATGTTTGAGGTTGGAGGAGCTCTTCAATTTTTCTTCAGTGCCAGTTGATTTTTGTGCTGAGTCTTTGCTACCTAAGCTAACTGTCATAAAACTCAAATATCTTCCACAATTAAGGAGCTTATGCAACGACAGAGTGGTGCTCGAATCCCTGGAACACTTAGAAGTCGAATCTTGTGAAAGTTTAAAGAACTTACCCTTTGTCCCCGGCAATACTGGTATGATCAACGAACAAATGGCGTGGGAATACATGTCTAGGACTCTAGGGTAA

Protein Analysis

919

Amino Acids

103.91

Weight (kDa)

6.64

Isoelectric Point (pI)

47.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 120 - 287 5.6e-38 NB-ARC domain
WHD_DRP PF23559 372 - 439 5e-11 Disease resistance protein Winged helix domain
LRR_8 PF13855 498 - 551 2.2e-07 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 507 - 572 4.1e-07 R13L1/DRL21 LRRs
LRR_14 PF23598 514 - 678 9.8e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 770 - 895 7.6e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011026)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27190 AT4G27220 AT4G27220
fragaria_vesca FvH4_3g21750
malus_domestica MD11G1227400.v1.1 MD11G1227500.v1.1
prunus_persica Prupe.4G201800_v2.0.a1
pyrus_communis pycom11g20010
rosa_chinensis RchiOBHm_Chr5g0037231
rosa_multiflora Rmu_sc0005002.1_g000030
rosa_roxburghii Rroxscaffold_1G00043740
rosa_rugosa Rorug05G0163900 Rorug05G0163900
rosa_samantha Rh5AG253500 Rh5BG255500 Rh5CG288200 Rh5CG288300 Rh5DG263900
rosa_wichuraiana Rw5G023420 Rw5G023450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1677, 2444
AccB1I GGYRCC 2 cut(s) 678, 2066
AccII CGCG 2 cut(s) 960, 1169
AccIII TCCGGA 1 cut(s) 1120
AciI CCGC 6 cut(s) 429, 949, 960, 1169, 1630, 1859
AclI AACGTT 1 cut(s) 852
AclWI GGATC 4 cut(s) 88, 101, 1578, 2301
AcoI YGGCCR 1 cut(s) 1365
AcsI RAATTY 5 cut(s) 225, 649, 997, 1507, 1991
AcuI CTGAAG 4 cut(s) 1053, 1395, 2366, 2511
AfaI GTAC 3 cut(s) 1051, 1160, 2458
AfiI CCNNNNNNNGG 2 cut(s) 1519, 2069
AflII CTTAAG 3 cut(s) 80, 1765, 2260
AflIII ACRYGT 2 cut(s) 2137, 2739
AgeI ACCGGT 1 cut(s) 2069
AjnI CCWGG 2 cut(s) 2062, 2640
AjuI GAANNNNNNNTTGG 6 cut(s) 20, 52, 690, 722, 1029, 1061
Alw21I GWGCWC 2 cut(s) 2514, 2634
Alw26I GTCTC 7 cut(s) 37, 565, 1638, 2110, 2371, 2410, 2494
AlwI GGATC 4 cut(s) 88, 101, 1578, 2301
Ama87I CYCGRG 2 cut(s) 2309, 2369
Aor13HI TCCGGA 1 cut(s) 1120
AoxI GGCC 3 cut(s) 259, 326, 1365
ApeKI GCWGC 3 cut(s) 485, 1761, 2412
ApoI RAATTY 5 cut(s) 225, 649, 997, 1507, 1991
AsiGI ACCGGT 1 cut(s) 2069
Asp700I GAANNNNTTC 2 cut(s) 650, 1347
AspLEI GCGC 1 cut(s) 960
AspS9I GGNCC 4 cut(s) 326, 550, 694, 951
AsuC2I CCSGG 2 cut(s) 1369, 2697
AsuHPI GGTGA 8 cut(s) 79, 176, 529, 1280, 1352, 2031, 2049, 2084
AvaI CYCGRG 2 cut(s) 2309, 2369
AvaII GGWCC 3 cut(s) 550, 694, 951
BamHI GGATCC 1 cut(s) 93
BanI GGYRCC 2 cut(s) 678, 2066
BanII GRGCYC 1 cut(s) 2514
BbsI GAAGAC 3 cut(s) 446, 766, 1323
Bbv12I GWGCWC 2 cut(s) 2514, 2634
BbvCI CCTCAGC 1 cut(s) 122
BbvI GCAGC 3 cut(s) 472, 1773, 2399
BccI CCATC 4 cut(s) 550, 1178, 1259, 1426
BciT130I CCWGG 2 cut(s) 2064, 2642
BciVI GTATCC 1 cut(s) 913
BclI TGATCA 1 cut(s) 2712
BcnI CCSGG 2 cut(s) 1369, 2697
BcoDI GTCTC 7 cut(s) 37, 565, 1638, 2110, 2371, 2410, 2494
BfrI CTTAAG 3 cut(s) 80, 1765, 2260
BfuAI ACCTGC 2 cut(s) 1677, 2444
BfuI GTATCC 1 cut(s) 913
BglII AGATCT 2 cut(s) 265, 1041
BisI GCNGC 3 cut(s) 486, 1762, 2413
BlpI GCTNAGC 1 cut(s) 206
BlsI GCNGC 3 cut(s) 487, 1763, 2414
Bme1390I CCNGG 4 cut(s) 1369, 2064, 2642, 2697
Bme18I GGWCC 3 cut(s) 550, 694, 951
BmeT110I CYCGRG 2 cut(s) 2309, 2369
BmgT120I GGNCC 4 cut(s) 326, 550, 694, 951
BmiI GGNNCC 7 cut(s) 95, 327, 551, 680, 953, 1667, 2068
BmrFI CCNGG 4 cut(s) 1369, 2064, 2642, 2697
BmrI ACTGGG 2 cut(s) 584, 1171
BmsI GCATC 3 cut(s) 1890, 2123, 2355
BmuI ACTGGG 2 cut(s) 584, 1171
BpiI GAAGAC 3 cut(s) 446, 766, 1323
BplI GAGNNNNNCTC 6 cut(s) 170, 202, 1115, 1147, 2616, 2648
BpmI CTGGAG 4 cut(s) 269, 377, 1784, 1811
Bpu10I CCTNAGC 2 cut(s) 122, 2565
Bpu1102I GCTNAGC 1 cut(s) 206
BpuEI CTTGAG 2 cut(s) 251, 954
BpuMI CCSGG 2 cut(s) 1369, 2697
Bsa29I ATCGAT 1 cut(s) 1026
BsaI GGTCTC 1 cut(s) 1638
BsaJI CCNNGG 4 cut(s) 63, 2368, 2640, 2695
BsaWI WCCGGW 2 cut(s) 1120, 2069
Bsc4I CCNNNNNNNGG 2 cut(s) 1519, 2069
Bse118I RCCGGY 1 cut(s) 2069
Bse1I ACTGG 8 cut(s) 360, 579, 1166, 1828, 2026, 2295, 2534, 2710
Bse3DI GCAATG 2 cut(s) 888, 1595
BseAI TCCGGA 1 cut(s) 1120
BseBI CCWGG 2 cut(s) 2064, 2642
BseCI ATCGAT 1 cut(s) 1026
BseDI CCNNGG 4 cut(s) 63, 2368, 2640, 2695
BseGI GGATG 4 cut(s) 337, 904, 1189, 2379
BseLI CCNNNNNNNGG 2 cut(s) 1519, 2069
BseMI GCAATG 2 cut(s) 888, 1595
BseMII CTCAG 7 cut(s) 113, 197, 1075, 1349, 1588, 2451, 2541
BseNI ACTGG 8 cut(s) 360, 579, 1166, 1828, 2026, 2295, 2534, 2710
BseRI GAGGAG 3 cut(s) 266, 1955, 2522
BseXI GCAGC 3 cut(s) 472, 1773, 2399
BseYI CCCAGC 1 cut(s) 2255
BsgI GTGCAG 1 cut(s) 77
Bsh1236I CGCG 2 cut(s) 960, 1169
Bsh1285I CGRYCG 2 cut(s) 728, 2051
BshFI GGCC 3 cut(s) 261, 328, 1367
BshNI GGYRCC 2 cut(s) 678, 2066
BshTI ACCGGT 1 cut(s) 2069
BshVI ATCGAT 1 cut(s) 1026
BsiEI CGRYCG 2 cut(s) 728, 2051
BsiHKAI GWGCWC 2 cut(s) 2514, 2634
BsiHKCI CYCGRG 2 cut(s) 2309, 2369
BsiSI CCGG 5 cut(s) 1121, 1368, 1811, 2070, 2697
BslFI GGGAC 2 cut(s) 2269, 2678
BslI CCNNNNNNNGG 2 cut(s) 1519, 2069
BsmAI GTCTC 7 cut(s) 37, 565, 1638, 2110, 2371, 2410, 2494
BsmFI GGGAC 2 cut(s) 2269, 2678
BsmI GAATGC 2 cut(s) 1547, 1806
BsnI GGCC 3 cut(s) 261, 328, 1367
Bso31I GGTCTC 1 cut(s) 1638
BsoBI CYCGRG 2 cut(s) 2309, 2369
Bsp1286I GDGCHC 2 cut(s) 2514, 2634
Bsp13I TCCGGA 1 cut(s) 1120
Bsp1720I GCTNAGC 1 cut(s) 206
BspACI CCGC 6 cut(s) 429, 949, 960, 1169, 1630, 1859
BspANI GGCC 3 cut(s) 261, 328, 1367
BspCNI CTCAG 7 cut(s) 114, 198, 1074, 1348, 1587, 2450, 2542
BspDI ATCGAT 1 cut(s) 1026
BspEI TCCGGA 1 cut(s) 1120
BspFNI CGCG 2 cut(s) 960, 1169
BspLI GGNNCC 7 cut(s) 95, 327, 551, 680, 953, 1667, 2068
BspMI ACCTGC 2 cut(s) 1677, 2444
BspPI GGATC 4 cut(s) 88, 101, 1578, 2301
BspQI GCTCTTC 3 cut(s) 193, 2198, 2519
BspT107I GGYRCC 2 cut(s) 678, 2066
BspTI CTTAAG 3 cut(s) 80, 1765, 2260
BspTNI GGTCTC 1 cut(s) 1638
BsrDI GCAATG 2 cut(s) 888, 1595
BsrFI RCCGGY 1 cut(s) 2069
BsrI ACTGG 8 cut(s) 360, 579, 1166, 1828, 2026, 2295, 2534, 2710
BssAI RCCGGY 1 cut(s) 2069
BssECI CCNNGG 4 cut(s) 63, 2368, 2640, 2695
BssT1I CCWWGG 1 cut(s) 63
Bst2UI CCWGG 2 cut(s) 2064, 2642
Bst4CI ACNGT 6 cut(s) 498, 523, 1285, 1652, 2238, 2575
Bst6I CTCTTC 4 cut(s) 141, 193, 2198, 2519
BstAFI CTTAAG 3 cut(s) 80, 1765, 2260
BstC8I GCNNGC 2 cut(s) 581, 811
BstF5I GGATG 4 cut(s) 337, 904, 1189, 2379
BstFNI CGCG 2 cut(s) 960, 1169
BstHHI GCGC 1 cut(s) 960
BstMAI GTCTC 7 cut(s) 37, 565, 1638, 2110, 2371, 2410, 2494
BstMCI CGRYCG 2 cut(s) 728, 2051
BstMWI GCNNNNNNNGC 6 cut(s) 253, 955, 1865, 2214, 2231, 2566
BstNI CCWGG 2 cut(s) 2064, 2642
BstNSI RCATGY 4 cut(s) 813, 1932, 2141, 2743
BstSCI CCNGG 4 cut(s) 1367, 2062, 2640, 2695
BstUI CGCG 2 cut(s) 960, 1169
BstV1I GCAGC 3 cut(s) 472, 1773, 2399
BstV2I GAAGAC 3 cut(s) 446, 766, 1323
BstX2I RGATCY 5 cut(s) 93, 265, 1041, 1570, 2293
BstXI CCANNNNNNTGG 3 cut(s) 574, 691, 854
BstYI RGATCY 5 cut(s) 93, 265, 1041, 1570, 2293
Bsu15I ATCGAT 1 cut(s) 1026
BsuI GTATCC 1 cut(s) 913
BsuRI GGCC 3 cut(s) 261, 328, 1367
BsuTUI ATCGAT 1 cut(s) 1026
BtsCI GGATG 4 cut(s) 337, 904, 1189, 2379
BtsIMutI CAGTG 6 cut(s) 353, 528, 1582, 2098, 2234, 2535
BveI ACCTGC 2 cut(s) 1677, 2444
Cac8I GCNNGC 2 cut(s) 581, 811
CfoI GCGC 1 cut(s) 960
Cfr10I RCCGGY 1 cut(s) 2069
Cfr13I GGNCC 4 cut(s) 326, 550, 694, 951
ClaI ATCGAT 1 cut(s) 1026
CseI GACGC 1 cut(s) 466
Csp6I GTAC 3 cut(s) 1050, 1159, 2457
CspAI ACCGGT 1 cut(s) 2069
CspCI CAANNNNNGTGG 2 cut(s) 2711, 2746
CviQI GTAC 3 cut(s) 1050, 1159, 2457
DraI TTTAAA 2 cut(s) 1056, 2676
EaeI YGGCCR 1 cut(s) 1365
Eam1104I CTCTTC 4 cut(s) 141, 193, 2198, 2519
EarI CTCTTC 4 cut(s) 141, 193, 2198, 2519
EciI GGCGGA 1 cut(s) 964
Ecl136II GAGCTC 1 cut(s) 2512
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 2514
Eco31I GGTCTC 1 cut(s) 1638
Eco47I GGWCC 3 cut(s) 550, 694, 951
Eco53kI GAGCTC 1 cut(s) 2512
Eco57I CTGAAG 4 cut(s) 1053, 1395, 2366, 2511
Eco88I CYCGRG 2 cut(s) 2309, 2369
EcoICRI GAGCTC 1 cut(s) 2512
EcoO109I RGGNCCY 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 1507
EcoRII CCWGG 2 cut(s) 2062, 2640
EcoT14I CCWWGG 1 cut(s) 63
EcoT22I ATGCAT 1 cut(s) 2116
EcoT38I GRGCYC 1 cut(s) 2514
ErhI CCWWGG 1 cut(s) 63
FalI AAGNNNNNCTT 2 cut(s) 1899, 1931
FaqI GGGAC 2 cut(s) 2269, 2678
FauI CCCGC 1 cut(s) 1852
FbaI TGATCA 1 cut(s) 2712
Fnu4HI GCNGC 3 cut(s) 486, 1762, 2413
FokI GGATG 4 cut(s) 324, 911, 1196, 2386
FriOI GRGCYC 1 cut(s) 2514
Fsp4HI GCNGC 3 cut(s) 486, 1762, 2413
GlaI GCGC 1 cut(s) 959
GluI GCNGC 3 cut(s) 486, 1762, 2413
GsaI CCCAGC 1 cut(s) 2259
GsuI CTGGAG 4 cut(s) 269, 377, 1784, 1811
HaeIII GGCC 3 cut(s) 261, 328, 1367
HapII CCGG 5 cut(s) 1121, 1368, 1811, 2070, 2697
HgaI GACGC 1 cut(s) 466
HhaI GCGC 1 cut(s) 960
Hin6I GCGC 1 cut(s) 958
HinP1I GCGC 1 cut(s) 958
HincII GTYRAC 1 cut(s) 289
HindII GTYRAC 1 cut(s) 289
HindIII AAGCTT 1 cut(s) 828
HpaII CCGG 5 cut(s) 1121, 1368, 1811, 2070, 2697
HphI GGTGA 8 cut(s) 79, 176, 529, 1280, 1352, 2031, 2049, 2084
Hpy166II GTNNAC 2 cut(s) 289, 1148
Hpy188I TCNGA 6 cut(s) 8, 874, 1740, 2351, 2385, 2440
Hpy8I GTNNAC 2 cut(s) 289, 1148
HpyAV CCTTC 9 cut(s) 339, 724, 1029, 1166, 1237, 1337, 1683, 1895, 2069
HpyCH4III ACNGT 6 cut(s) 498, 523, 1285, 1652, 2238, 2575
HpyCH4IV ACGT 2 cut(s) 852, 2428
HpyCH4V TGCA 8 cut(s) 58, 809, 893, 1294, 1945, 2114, 2134, 2616
HpyF10VI GCNNNNNNNGC 6 cut(s) 253, 955, 1865, 2214, 2231, 2566
HpySE526I ACGT 2 cut(s) 852, 2428
HspAI GCGC 1 cut(s) 958
Kpn2I TCCGGA 1 cut(s) 1120
Ksp22I TGATCA 1 cut(s) 2712
LguI GCTCTTC 3 cut(s) 193, 2198, 2519
LmnI GCTCC 6 cut(s) 112, 253, 595, 1968, 2509, 2607
Lsp1109I GCAGC 3 cut(s) 472, 1773, 2399
LweI GCATC 3 cut(s) 1890, 2123, 2355
MaeII ACGT 2 cut(s) 852, 2428
MaeIII GTNAC 6 cut(s) 517, 1222, 1307, 1924, 2140, 2197
MfeI CAATTG 1 cut(s) 698
MflI RGATCY 5 cut(s) 93, 265, 1041, 1570, 2293
MhlI GDGCHC 2 cut(s) 2514, 2634
MlyI GAGTC 5 cut(s) 1241, 1598, 2291, 2561, 2743
MmeI TCCRAC 5 cut(s) 345, 835, 1161, 1809, 2484
Mph1103I ATGCAT 1 cut(s) 2116
MroI TCCGGA 1 cut(s) 1120
MroXI GAANNNNTTC 2 cut(s) 650, 1347
MspCI CTTAAG 3 cut(s) 80, 1765, 2260
MspI CCGG 5 cut(s) 1121, 1368, 1811, 2070, 2697
MspR9I CCNGG 4 cut(s) 1369, 2064, 2642, 2697
MunI CAATTG 1 cut(s) 698
Mva1269I GAATGC 2 cut(s) 1547, 1806
MvaI CCWGG 2 cut(s) 2064, 2642
MvnI CGCG 2 cut(s) 960, 1169
MwoI GCNNNNNNNGC 6 cut(s) 253, 955, 1865, 2214, 2231, 2566
NciI CCSGG 2 cut(s) 1369, 2697
NlaIV GGNNCC 7 cut(s) 95, 327, 551, 680, 953, 1667, 2068
NmuCI GTSAC 3 cut(s) 517, 1307, 2140
NsiI ATGCAT 1 cut(s) 2116
NspI RCATGY 4 cut(s) 813, 1932, 2141, 2743
PaeI GCATGC 1 cut(s) 813
PaeR7I CTCGAG 1 cut(s) 2309
PciI ACATGT 2 cut(s) 2137, 2739
PciSI GCTCTTC 3 cut(s) 193, 2198, 2519
PctI GAATGC 2 cut(s) 1547, 1806
PdmI GAANNNNTTC 2 cut(s) 650, 1347
PfeI GAWTC 8 cut(s) 604, 734, 1563, 1682, 1718, 1997, 2636, 2660
PflFI GACNNNGTC 2 cut(s) 523, 2330
PinAI ACCGGT 1 cut(s) 2069
PkrI GCNGC 3 cut(s) 487, 1763, 2414
Ple19I CGATCG 2 cut(s) 728, 2051
PleI GAGTC 5 cut(s) 1241, 1598, 2290, 2560, 2743
PpsI GAGTC 5 cut(s) 1241, 1598, 2290, 2560, 2743
PscI ACATGT 2 cut(s) 2137, 2739
Psp124BI GAGCTC 1 cut(s) 2514
Psp1406I AACGTT 1 cut(s) 852
Psp6I CCWGG 2 cut(s) 2062, 2640
PspFI CCCAGC 1 cut(s) 2255
PspGI CCWGG 2 cut(s) 2062, 2640
PspN4I GGNNCC 7 cut(s) 95, 327, 551, 680, 953, 1667, 2068
PspPI GGNCC 4 cut(s) 326, 550, 694, 951
PsuI RGATCY 5 cut(s) 93, 265, 1041, 1570, 2293
PsyI GACNNNGTC 2 cut(s) 523, 2330
PvuI CGATCG 2 cut(s) 728, 2051
RsaI GTAC 3 cut(s) 1051, 1160, 2458
RsaNI GTAC 3 cut(s) 1050, 1159, 2457
SacI GAGCTC 1 cut(s) 2514
SapI GCTCTTC 3 cut(s) 193, 2198, 2519
SatI GCNGC 3 cut(s) 486, 1762, 2413
Sau96I GGNCC 4 cut(s) 326, 550, 694, 951
SchI GAGTC 5 cut(s) 1241, 1598, 2291, 2561, 2743
ScrFI CCNGG 4 cut(s) 1369, 2064, 2642, 2697
SduI GDGCHC 2 cut(s) 2514, 2634
SfaNI GCATC 3 cut(s) 1890, 2123, 2355
Sfr274I CTCGAG 1 cut(s) 2309
SgrAI CRCCGGYG 1 cut(s) 2069
SinI GGWCC 3 cut(s) 550, 694, 951
SlaI CTCGAG 1 cut(s) 2309
SmlI CTYRAG 6 cut(s) 80, 230, 969, 1765, 2260, 2309
SmoI CTYRAG 6 cut(s) 80, 230, 969, 1765, 2260, 2309
SphI GCATGC 1 cut(s) 813
SsiI CCGC 6 cut(s) 429, 949, 960, 1169, 1630, 1859
SstI GAGCTC 1 cut(s) 2514
StyD4I CCNGG 4 cut(s) 1367, 2062, 2640, 2695
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 6 cut(s) 498, 523, 1285, 1652, 2238, 2575
TaiI ACGT 2 cut(s) 855, 2431
TatI WGTACW 1 cut(s) 1158
TfiI GAWTC 8 cut(s) 604, 734, 1563, 1682, 1718, 1997, 2636, 2660
TscAI CASTG 6 cut(s) 360, 528, 1582, 2098, 2241, 2535
TseFI GTSAC 3 cut(s) 517, 1307, 2140
TseI GCWGC 3 cut(s) 485, 1761, 2412
Tsp45I GTSAC 3 cut(s) 517, 1307, 2140
TspRI CASTG 6 cut(s) 360, 528, 1582, 2098, 2241, 2535
Tth111I GACNNNGTC 2 cut(s) 523, 2330
Vha464I CTTAAG 3 cut(s) 80, 1765, 2260
VpaK11BI GGWCC 3 cut(s) 550, 694, 951
XapI RAATTY 5 cut(s) 225, 649, 997, 1507, 1991
XbaI TCTAGA 2 cut(s) 299, 1849
XceI RCATGY 4 cut(s) 813, 1932, 2141, 2743
XcmI CCANNNNNNNNNTGG 1 cut(s) 914
XhoI CTCGAG 1 cut(s) 2309
XmnI GAANNNNTTC 2 cut(s) 650, 1347
Zsp2I ATGCAT 1 cut(s) 2116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.