Rroxscaffold_1G00043740

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0011026
Physical Location & Seq
Forward (+)
62451111 .. 62455736
4626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00043740.1

Sequence Viewer

Length: 2985 bp
ATGGAAACTTTGGGTGCTTTCTTTGTTGAAGTTTGCAAGTCTTTAACCAAATTCATGTGGTCGAAGTTTGCCACCCTGAAAAAATACCCAAAAAACATCCAAACATTGAAGAAGGAGCTGCAAAGCCTCAGTTGCCGAAGGGATGAGATAACAGATGATATTGATATAGCAAAACTAGAAGGGAAGTTCCCAGCAAGGCAAGTTGTGGACTGGCTTAAGAAGGTTGAAGCCATCGAACGTGCTGTTGAGGAGGAGATTGACCGTAAAGTGCAGCCATTTTTGCAAGAAGTTCATATTGATGCTTCTGTTAATGGCCAGGGATGTCTTTTTGACTCTAACATGCGCCAGAGGTACCATCTTAGCAAAACTGCAGCAAAGAAGTATGATGAGGTAAAACAACTAATTACTGAATCATGCAATTTTCCTACTATGTGGGATGCTTTGAGGCTTCGTGATATACCCATTCAGCATATTCCGGCACCATCTCTTGTTGGTCAGAAAGCACAGGAGAAGCTAAAGCAACTGATGGAATTTCTGGCTGATACTAAGATCAGCAGGATTGCTGTCTATGGAATGGGAGGTTCTGGGAAGACTACGCTTGTAAAAACCTTGAACAATCAGCTCCAGTCTTCTGCTTCAAAGTTATGTGATATGGTCTTCTGGATCCCTGTCTCAAATGACTTGGATATGAGTAAGGTTCAATCTCGAGTTGCTGAGAGATTGAACTTAGCAATGAATGCAGAAGAGAGCATAGAGTGTAGGGCTTGTAGATTACATCAAGCATTAAAGTCAGGAAAAAGGTTCCTCCTTATTCTTGATGATGTTTGGGAGAAAATTGACTTGGATGCCGTGGGGATACCACAAGGTGATGACCAAGCAAACTGCAAGATCGTAATGACAACTCGATCTCTTGCCGTATGTAGGGAAATGATGACTGATAAGGAACTAAAGATGGAACTTCTGAATGAGGAAGAGGCTTGGAATTTATTTGCACGGAATGCAGGAATTGTAGTGGAGTCAGAACACATAAATCCTCTAGCAAGAGCAATTGCTAGCGAATGCGGTGGTTTGCCACTGGCAATTGAGACTATGGGGAAGTCAATGAGGGACAAAACAATGATACAGCTATGGAAGAATGCACTATGCCAGTTGAAGCATTCAGCATCACATTATGGGAGCTTTGACAAGGTGCATCTGCGACTGGAATTGAGTTATAACTCTTTACCAAGTAAGATTCTTAAGAGGTGTTTCTTGTTTTGTTCATTGTACCCAGAAAATTTCTTAATTACTGCAAGAGAACTAATATGTGGCTGGATAGCGGATGGATTAATTATTGAGCATCAAACGTTAGAGGAGTCATTCAATGATGGGATTGCAAAAATTGAATATTTGAAAGACTCTTGCATGCTAGAACAGGGTGAATGCATTGGAACAGTAAAGATGCACAGTGTATTGAGGGAAGTAGCCTTGTGGATATCCTCAAATAGAAAAGAAACCGGGTTTTTCAGCCGCTCATTGCTGGGAATGCAAGAAAAGTTGGAGAGTTCTATCAGAGGAGTTTCACTTATGGAGAAATCTATAACCAGTTTGCCAAGTCGTTTTTCAGGGTGCTCCAACCTGACTGTATTGTTCCTCCAATGTAATCCTCTGTATAAAATTCCTGATGGTTTCTTTCGAGAACTTAGAGCCCTAAGATTCTTGAATCTAAGCAGCACTCAAATATCTTCCTTGCCTTCTTCTCTTCTTCAACTAGCAGAGCTACACACTCTTCTCTTAAGAGACTGTTCTTCCCTAGAAAATCTGCCTCAAATTGGAGCTCTTTATAAACTCCAGGTGCTTGATCTCTCTGGCACTCGCATAAGAGAACTTCCAAAAGACATGGGAACGTTAACTCATTTGAGAGATCTAAACTTGTCGCGCACAAACCACTTAGAAAGTATCATAGCTGGAAGCATCTCAGGTTTATATAGTCTTGAGGCCTTGGACATGTCTTCCAGTGCTTATAAGTGGGATTTGGAACGTAACGTAGAAGGAGCACCATTTGATGAAATTCTATCCTTACAGGGACTTTCAGTTCTTCACATAAGGCTAGACACAGTTAATTGTGTTGCCTTAGACTCGGATGGCTCTTGGTTTGGAAGATTGAAAGAGTTTTCTATTTGGATTGGTCCAAGGAGCTGCGATTCAAGCTATTTACCAACTCAGCATGATGAGAAAAGGGTCATACTTAGAGGGGTTGACCTCTTGCAAAGTGGCCTCGAGGGGTTATTGTACAGTGCAAGTTCCCTGGATCTGGTCACATGTGGAGGCATAAGTAGTTTATCTGATCTAATTTCCAGCAGGAAACTGGGTGGCCTGCCAAACTTGAAGTTTCTCACCATCTCAAATTGTGACTGCATTACTAGTTTGTTAATTGGCGAAGAAACTCTTGGGAGCACATTGTCAAATCTGGAGCATTTGACCCTCAATTGCCTAAACAACTTGGAAACCATGGTGGACAGGATAGTCCCTAGAGGATGCCTTGGCAAATTAAAGAAGATTGAAGTGATGGGATGCCGAAGACTAAAGACTCTCATATCCTTCACTTTACTTCGACGGGTTCAAAATGTTGAAGAAATCAAGGTGAGTGACTGCAGAAGGATGAAGAAAATTATTGTAAACATCAATTTGTGCGAAACTCTTCCAAAGCTGAAATACATAGAACTAAGGAATATGGACAGCTTAACAGCTATTTGTTCAAAGGCTGCAAAGTGGTCTGCTCTGAAAAGGATTGAAGTGAGCGATTGCCCCAAGCTAGAGAAGCTTCCACTAAGAGCCCAGAATGCAGCAAATCTTAAAGAAATTAGAGGGGACTTGAGTTGGTGGAATGGTCTTAGATGGGAAAATGATGGAGACAAGATCAGTCTTGAGCAACGATTCCAAGCGTGTGCAGACTCCACACTGCTGTTAAGAGAGGATACAGAGGTAACAAAAATGTACTCATAA

Protein Analysis

994

Amino Acids

112.15

Weight (kDa)

7.62

Isoelectric Point (pI)

47.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 170 - 333 2e-38 NB-ARC domain
LRR_14 PF23598 517 - 734 3.7e-17 Leucine-rich repeat region
LRR_8 PF13855 538 - 595 2.2e-08 Leucine rich repeat
LRR_8 PF13855 609 - 665 1.7e-07 Leucine rich repeat
LRR_RPS2 PF23247 760 - 879 1.5e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 836 - 935 2.1e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011026)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27190 AT4G27220 AT4G27220
fragaria_vesca FvH4_3g21750
malus_domestica MD11G1227400.v1.1 MD11G1227500.v1.1
prunus_persica Prupe.4G201800_v2.0.a1
pyrus_communis pycom11g20010
rosa_chinensis RchiOBHm_Chr5g0037231
rosa_multiflora Rmu_sc0005002.1_g000030
rosa_roxburghii Rroxscaffold_1G00043740
rosa_rugosa Rorug05G0163900 Rorug05G0163900
rosa_samantha Rh5AG253500 Rh5BG255500 Rh5CG288200 Rh5CG288300 Rh5DG263900
rosa_wichuraiana Rw5G023420 Rw5G023450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1215, 1824, 2004
AasI GACNNNNNNGTC 1 cut(s) 2504
AbsI CCTCGAGG 1 cut(s) 2258
Acc65I GGTACC 1 cut(s) 351
AccB1I GGYRCC 2 cut(s) 351, 478
AccBSI CCGCTC 1 cut(s) 1512
AccII CGCG 1 cut(s) 1918
AciI CCGC 3 cut(s) 1062, 1319, 1510
AclI AACGTT 2 cut(s) 1346, 1886
AclWI GGATC 3 cut(s) 658, 671, 2298
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 6 cut(s) 50, 530, 982, 1276, 1656, 2049
AdeI CACNNNGTG 1 cut(s) 866
AfaI GTAC 4 cut(s) 353, 1268, 2273, 2978
AfiI CCNNNNNNNGG 3 cut(s) 921, 1811, 2293
AflII CTTAAG 3 cut(s) 215, 1238, 1774
AflIII ACRYGT 2 cut(s) 1986, 2300
AhlI ACTAGT 1 cut(s) 2402
AjnI CCWGG 3 cut(s) 315, 1830, 2286
AjuI GAANNNNNNNTTGG 6 cut(s) 824, 856, 1585, 1617, 2412, 2444
AloI GAACNNNNNNTCC 2 cut(s) 2058, 2090
Alw21I GWGCWC 4 cut(s) 1613, 1819, 2038, 2438
Alw26I GTCTC 4 cut(s) 676, 1079, 1773, 2886
AlwI GGATC 3 cut(s) 658, 671, 2298
Ama87I CYCGRG 2 cut(s) 705, 2258
AoxI GGCC 4 cut(s) 313, 1977, 2254, 2353
ApeKI GCWGC 7 cut(s) 118, 271, 371, 1710, 2178, 2744, 2825
ApoI RAATTY 6 cut(s) 50, 530, 982, 1276, 1656, 2049
AseI ATTAAT 1 cut(s) 1328
Asp700I GAANNNNTTC 1 cut(s) 2679
Asp718I GGTACC 1 cut(s) 351
AspLEI GCGC 2 cut(s) 345, 1920
AspS9I GGNCC 1 cut(s) 2168
AsuC2I CCSGG 1 cut(s) 1498
AsuHPI GGTGA 4 cut(s) 878, 1430, 2368, 2635
AsuNHI GCTAGC 1 cut(s) 1052
AvaI CYCGRG 2 cut(s) 705, 2258
AvaII GGWCC 1 cut(s) 2168
BaeI ACNNNNGTAYC 2 cut(s) 2949, 2982
BalI TGGCCA 1 cut(s) 315
BamHI GGATCC 1 cut(s) 663
BanI GGYRCC 2 cut(s) 351, 478
BanII GRGCYC 3 cut(s) 1690, 1819, 2818
BbsI GAAGAC 5 cut(s) 596, 621, 649, 1983, 2566
Bbv12I GWGCWC 4 cut(s) 1613, 1819, 2038, 2438
BbvI GCAGC 7 cut(s) 105, 283, 383, 1722, 2165, 2731, 2837
BceAI ACGGC 2 cut(s) 833, 899
BciT130I CCWGG 3 cut(s) 317, 1832, 2288
BciVI GTATCC 2 cut(s) 849, 2950
BcnI CCSGG 1 cut(s) 1498
BcoDI GTCTC 4 cut(s) 676, 1079, 1773, 2886
BcuI ACTAGT 1 cut(s) 2402
BfmI CTRYAG 2 cut(s) 369, 2632
BfrI CTTAAG 3 cut(s) 215, 1238, 1774
BfuI GTATCC 2 cut(s) 849, 2950
BglII AGATCT 1 cut(s) 1903
BisI GCNGC 8 cut(s) 119, 272, 372, 1510, 1711, 2179, 2745, 2826
BlsI GCNGC 8 cut(s) 120, 273, 373, 1511, 1712, 2180, 2746, 2827
Bme1390I CCNGG 4 cut(s) 317, 1498, 1832, 2288
Bme18I GGWCC 1 cut(s) 2168
BmeT110I CYCGRG 2 cut(s) 705, 2258
BmgT120I GGNCC 1 cut(s) 2168
BmiI GGNNCC 4 cut(s) 353, 480, 665, 803
BmrFI CCNGG 4 cut(s) 317, 1498, 1832, 2288
BmrI ACTGGG 1 cut(s) 2357
BmtI GCTAGC 1 cut(s) 1056
BmuI ACTGGG 1 cut(s) 2357
BpiI GAAGAC 5 cut(s) 596, 621, 649, 1983, 2566
BpmI CTGGAG 3 cut(s) 608, 1814, 2471
BpuEI CTTGAG 3 cut(s) 1994, 2875, 2927
BpuMI CCSGG 1 cut(s) 1498
BsaJI CCNNGG 7 cut(s) 316, 849, 1980, 2171, 2286, 2490, 2521
Bsc4I CCNNNNNNNGG 3 cut(s) 921, 1811, 2293
Bse1I ACTGG 8 cut(s) 215, 625, 1080, 1147, 1206, 1584, 1995, 2352
Bse3DI GCAATG 2 cut(s) 738, 1514
BseBI CCWGG 3 cut(s) 317, 1832, 2288
BseDI CCNNGG 7 cut(s) 316, 849, 1980, 2171, 2286, 2490, 2521
BseLI CCNNNNNNNGG 3 cut(s) 921, 1811, 2293
BseMI GCAATG 2 cut(s) 738, 1514
BseMII CTCAG 4 cut(s) 142, 705, 1971, 2216
BseNI ACTGG 8 cut(s) 215, 625, 1080, 1147, 1206, 1584, 1995, 2352
BseRI GAGGAG 4 cut(s) 263, 266, 1367, 1569
BseXI GCAGC 7 cut(s) 105, 283, 383, 1722, 2165, 2731, 2837
BseYI CCCAGC 2 cut(s) 190, 1519
BsgI GTGCAG 2 cut(s) 290, 2949
Bsh1236I CGCG 1 cut(s) 1918
BshFI GGCC 4 cut(s) 315, 1979, 2256, 2355
BshNI GGYRCC 2 cut(s) 351, 478
BsiHKAI GWGCWC 4 cut(s) 1613, 1819, 2038, 2438
BsiHKCI CYCGRG 2 cut(s) 705, 2258
BsiSI CCGG 2 cut(s) 476, 1497
BslFI GGGAC 4 cut(s) 1121, 2079, 2492, 2864
BslI CCNNNNNNNGG 3 cut(s) 921, 1811, 2293
BsmAI GTCTC 4 cut(s) 676, 1079, 1773, 2886
BsmFI GGGAC 4 cut(s) 1121, 2079, 2492, 2864
BsmI GAATGC 8 cut(s) 742, 1003, 1064, 1141, 1156, 1427, 1530, 2827
BsnI GGCC 4 cut(s) 315, 1979, 2256, 2355
BsoBI CYCGRG 2 cut(s) 705, 2258
Bsp1286I GDGCHC 6 cut(s) 1613, 1690, 1819, 2038, 2438, 2818
Bsp1407I TGTACA 1 cut(s) 2271
Bsp143I GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2326, 2898
Bsp19I CCATGG 1 cut(s) 2490
BspACI CCGC 3 cut(s) 1062, 1319, 1510
BspANI GGCC 4 cut(s) 315, 1979, 2256, 2355
BspCNI CTCAG 4 cut(s) 141, 706, 1970, 2215
BspFNI CGCG 1 cut(s) 1918
BspLI GGNNCC 4 cut(s) 353, 480, 665, 803
BspMAI CTGCAG 2 cut(s) 373, 2636
BspOI GCTAGC 1 cut(s) 1056
BspPI GGATC 3 cut(s) 658, 671, 2298
BspT107I GGYRCC 2 cut(s) 351, 478
BspTI CTTAAG 3 cut(s) 215, 1238, 1774
BsrBI CCGCTC 1 cut(s) 1512
BsrDI GCAATG 2 cut(s) 738, 1514
BsrGI TGTACA 1 cut(s) 2271
BsrI ACTGG 8 cut(s) 215, 625, 1080, 1147, 1206, 1584, 1995, 2352
BssECI CCNNGG 7 cut(s) 316, 849, 1980, 2171, 2286, 2490, 2521
BssMI GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2326, 2898
BssT1I CCWWGG 4 cut(s) 1980, 2171, 2490, 2521
Bst2UI CCWGG 3 cut(s) 317, 1832, 2288
Bst4CI ACNGT 7 cut(s) 263, 1435, 1448, 1624, 1784, 2098, 2276
Bst6I CTCTTC 5 cut(s) 738, 966, 1746, 1773, 2685
BstAFI CTTAAG 3 cut(s) 215, 1238, 1774
BstAPI GCANNNNNTGC 2 cut(s) 737, 998
BstAUI TGTACA 1 cut(s) 2271
BstC8I GCNNGC 3 cut(s) 1054, 1406, 2357
BstDSI CCRYGG 2 cut(s) 849, 2490
BstFNI CGCG 1 cut(s) 1918
BstHHI GCGC 2 cut(s) 345, 1920
BstKTI GATC 9 cut(s) 552, 666, 891, 908, 1843, 1906, 2293, 2329, 2901
BstMAI GTCTC 4 cut(s) 676, 1079, 1773, 2886
BstMBI GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2326, 2898
BstMWI GCNNNNNNNGC 8 cut(s) 132, 280, 737, 998, 1525, 2187, 2800, 2822
BstNI CCWGG 3 cut(s) 317, 1832, 2288
BstNSI RCATGY 4 cut(s) 343, 1408, 1990, 2304
BstSCI CCNGG 4 cut(s) 315, 1496, 1830, 2286
BstSFI CTRYAG 2 cut(s) 369, 2632
BstUI CGCG 1 cut(s) 1918
BstV1I GCAGC 7 cut(s) 105, 283, 383, 1722, 2165, 2731, 2837
BstV2I GAAGAC 5 cut(s) 596, 621, 649, 1983, 2566
BstX2I RGATCY 3 cut(s) 663, 1903, 2290
BstYI RGATCY 3 cut(s) 663, 1903, 2290
BsuI GTATCC 2 cut(s) 849, 2950
BsuRI GGCC 4 cut(s) 315, 1979, 2256, 2355
BtgI CCRYGG 2 cut(s) 849, 2490
BtsI GCAGTG 1 cut(s) 2939
BtsIMutI CAGTG 5 cut(s) 1073, 1453, 2002, 2281, 2939
Cac8I GCNNGC 3 cut(s) 1054, 1406, 2357
CfoI GCGC 2 cut(s) 345, 1920
Cfr13I GGNCC 1 cut(s) 2168
Csp6I GTAC 4 cut(s) 352, 1267, 2272, 2977
CviAII CATG 9 cut(s) 55, 340, 414, 1405, 1879, 1987, 2207, 2301, 2491
CviQI GTAC 4 cut(s) 352, 1267, 2272, 2977
DpnI GATC 9 cut(s) 551, 665, 890, 907, 1842, 1905, 2292, 2328, 2900
DpnII GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2326, 2898
DraIII CACNNNGTG 1 cut(s) 866
DrdI GACNNNNNNGTC 1 cut(s) 2504
DseDI GACNNNNNNGTC 1 cut(s) 2504
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 5 cut(s) 738, 966, 1746, 1773, 2685
EarI CTCTTC 5 cut(s) 738, 966, 1746, 1773, 2685
Ecl136II GAGCTC 1 cut(s) 1817
Eco130I CCWWGG 4 cut(s) 1980, 2171, 2490, 2521
Eco147I AGGCCT 1 cut(s) 1979
Eco24I GRGCYC 3 cut(s) 1690, 1819, 2818
Eco32I GATATC 1 cut(s) 1476
Eco47I GGWCC 1 cut(s) 2168
Eco53kI GAGCTC 1 cut(s) 1817
Eco88I CYCGRG 2 cut(s) 705, 2258
EcoICRI GAGCTC 1 cut(s) 1817
EcoRII CCWGG 3 cut(s) 315, 1830, 2286
EcoRV GATATC 1 cut(s) 1476
EcoT14I CCWWGG 4 cut(s) 1980, 2171, 2490, 2521
EcoT22I ATGCAT 1 cut(s) 1427
EcoT38I GRGCYC 3 cut(s) 1690, 1819, 2818
ErhI CCWWGG 4 cut(s) 1980, 2171, 2490, 2521
FaeI CATG 9 cut(s) 58, 343, 417, 1408, 1882, 1990, 2210, 2304, 2494
FalI AAGNNNNNCTT 2 cut(s) 2412, 2444
FaqI GGGAC 4 cut(s) 1121, 2079, 2492, 2864
FatI CATG 9 cut(s) 54, 339, 413, 1404, 1878, 1986, 2206, 2300, 2490
Fnu4HI GCNGC 8 cut(s) 119, 272, 372, 1510, 1711, 2179, 2745, 2826
FriOI GRGCYC 3 cut(s) 1690, 1819, 2818
Fsp4HI GCNGC 8 cut(s) 119, 272, 372, 1510, 1711, 2179, 2745, 2826
GlaI GCGC 2 cut(s) 344, 1919
GluI GCNGC 8 cut(s) 119, 272, 372, 1510, 1711, 2179, 2745, 2826
GsaI CCCAGC 2 cut(s) 194, 1523
GsuI CTGGAG 3 cut(s) 608, 1814, 2471
HaeIII GGCC 4 cut(s) 315, 1979, 2256, 2355
HapII CCGG 2 cut(s) 476, 1497
HhaI GCGC 2 cut(s) 345, 1920
Hin1II CATG 9 cut(s) 58, 343, 417, 1408, 1882, 1990, 2210, 2304, 2494
Hin6I GCGC 2 cut(s) 343, 1918
HinP1I GCGC 2 cut(s) 343, 1918
HincII GTYRAC 2 cut(s) 1890, 2239
HindII GTYRAC 2 cut(s) 1890, 2239
HindIII AAGCTT 1 cut(s) 2801
HpaI GTTAAC 1 cut(s) 1890
HpaII CCGG 2 cut(s) 476, 1497
HphI GGTGA 4 cut(s) 878, 1430, 2368, 2635
Hpy166II GTNNAC 5 cut(s) 208, 1890, 2239, 2497, 2659
Hpy188I TCNGA 7 cut(s) 498, 963, 1021, 1553, 2122, 2326, 2763
Hpy8I GTNNAC 5 cut(s) 208, 1890, 2239, 2497, 2659
Hpy99I CGWCG 1 cut(s) 2598
HpyAV CCTTC 8 cut(s) 106, 132, 173, 214, 1743, 2024, 2590, 2631
HpyCH4III ACNGT 7 cut(s) 263, 1435, 1448, 1624, 1784, 2098, 2276
HpyCH4IV ACGT 5 cut(s) 238, 1346, 1886, 2020, 2025
HpyF10VI GCNNNNNNNGC 8 cut(s) 132, 280, 737, 998, 1525, 2187, 2800, 2822
HpySE526I ACGT 5 cut(s) 238, 1346, 1886, 2020, 2025
Hsp92II CATG 9 cut(s) 58, 343, 417, 1408, 1882, 1990, 2210, 2304, 2494
HspAI GCGC 2 cut(s) 343, 1918
KpnI GGTACC 1 cut(s) 355
KspAI GTTAAC 1 cut(s) 1890
Kzo9I GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2326, 2898
LmnI GCTCC 9 cut(s) 115, 627, 1176, 1616, 1814, 2033, 2175, 2433, 2452
Lsp1109I GCAGC 7 cut(s) 105, 283, 383, 1722, 2165, 2731, 2837
MaeII ACGT 5 cut(s) 238, 1346, 1886, 2020, 2025
MaeIII GTNAC 5 cut(s) 2021, 2296, 2390, 2627, 2965
MalI GATC 9 cut(s) 551, 665, 890, 907, 1842, 1905, 2292, 2328, 2900
MbiI CCGCTC 1 cut(s) 1512
MboI GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2326, 2898
MfeI CAATTG 3 cut(s) 1047, 1080, 2467
MflI RGATCY 3 cut(s) 663, 1903, 2290
MhlI GDGCHC 6 cut(s) 1613, 1690, 1819, 2038, 2438, 2818
MlsI TGGCCA 1 cut(s) 315
MluNI TGGCCA 1 cut(s) 315
MlyI GAGTC 7 cut(s) 326, 1025, 1364, 1391, 2111, 2563, 2927
MmeI TCCRAC 2 cut(s) 1518, 1638
Mox20I TGGCCA 1 cut(s) 315
Mph1103I ATGCAT 1 cut(s) 1427
MroXI GAANNNNTTC 1 cut(s) 2679
MscI TGGCCA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 297
Msp20I TGGCCA 1 cut(s) 315
MspCI CTTAAG 3 cut(s) 215, 1238, 1774
MspI CCGG 2 cut(s) 476, 1497
MspR9I CCNGG 4 cut(s) 317, 1498, 1832, 2288
MunI CAATTG 3 cut(s) 1047, 1080, 2467
Mva1269I GAATGC 8 cut(s) 742, 1003, 1064, 1141, 1156, 1427, 1530, 2827
MvaI CCWGG 3 cut(s) 317, 1832, 2288
MvnI CGCG 1 cut(s) 1918
MwoI GCNNNNNNNGC 8 cut(s) 132, 280, 737, 998, 1525, 2187, 2800, 2822
NciI CCSGG 1 cut(s) 1498
NcoI CCATGG 1 cut(s) 2490
NdeII GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2326, 2898
NheI GCTAGC 1 cut(s) 1052
NlaIII CATG 9 cut(s) 58, 343, 417, 1408, 1882, 1990, 2210, 2304, 2494
NlaIV GGNNCC 4 cut(s) 353, 480, 665, 803
NmuCI GTSAC 3 cut(s) 2296, 2390, 2627
NsiI ATGCAT 1 cut(s) 1427
NspI RCATGY 4 cut(s) 343, 1408, 1990, 2304
PaeI GCATGC 1 cut(s) 1408
PaeR7I CTCGAG 2 cut(s) 705, 2258
PceI AGGCCT 1 cut(s) 1979
PciI ACATGT 2 cut(s) 1986, 2300
PctI GAATGC 8 cut(s) 742, 1003, 1064, 1141, 1156, 1427, 1530, 2827
PdmI GAANNNNTTC 1 cut(s) 2679
PfeI GAWTC 6 cut(s) 410, 1234, 1695, 1702, 2183, 2916
PkrI GCNGC 8 cut(s) 120, 273, 373, 1511, 1712, 2180, 2746, 2827
PleI GAGTC 7 cut(s) 326, 1024, 1363, 1391, 2111, 2563, 2927
PpsI GAGTC 7 cut(s) 326, 1024, 1363, 1391, 2111, 2563, 2927
PscI ACATGT 2 cut(s) 1986, 2300
PshBI ATTAAT 1 cut(s) 1328
PsiI TTATAA 3 cut(s) 1215, 1824, 2004
Psp124BI GAGCTC 1 cut(s) 1819
Psp1406I AACGTT 2 cut(s) 1346, 1886
Psp6I CCWGG 3 cut(s) 315, 1830, 2286
PspFI CCCAGC 2 cut(s) 190, 1519
PspGI CCWGG 3 cut(s) 315, 1830, 2286
PspN4I GGNNCC 4 cut(s) 353, 480, 665, 803
PspPI GGNCC 1 cut(s) 2168
PspXI VCTCGAGB 1 cut(s) 2258
PstI CTGCAG 2 cut(s) 373, 2636
PsuI RGATCY 3 cut(s) 663, 1903, 2290
RsaI GTAC 4 cut(s) 353, 1268, 2273, 2978
RsaNI GTAC 4 cut(s) 352, 1267, 2272, 2977
RseI CAYNNNNRTG 1 cut(s) 297
SacI GAGCTC 1 cut(s) 1819
SatI GCNGC 8 cut(s) 119, 272, 372, 1510, 1711, 2179, 2745, 2826
Sau3AI GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2326, 2898
Sau96I GGNCC 1 cut(s) 2168
SchI GAGTC 7 cut(s) 326, 1025, 1364, 1391, 2111, 2563, 2927
ScrFI CCNGG 4 cut(s) 317, 1498, 1832, 2288
SduI GDGCHC 6 cut(s) 1613, 1690, 1819, 2038, 2438, 2818
SfcI CTRYAG 2 cut(s) 369, 2632
Sfr274I CTCGAG 2 cut(s) 705, 2258
SinI GGWCC 1 cut(s) 2168
SlaI CTCGAG 2 cut(s) 705, 2258
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 8 cut(s) 215, 705, 1238, 1774, 1973, 2258, 2854, 2906
SmoI CTYRAG 8 cut(s) 215, 705, 1238, 1774, 1973, 2258, 2854, 2906
SpeI ACTAGT 1 cut(s) 2402
SphI GCATGC 1 cut(s) 1408
SseBI AGGCCT 1 cut(s) 1979
SsiI CCGC 3 cut(s) 1062, 1319, 1510
SspI AATATT 1 cut(s) 1388
SstI GAGCTC 1 cut(s) 1819
StuI AGGCCT 1 cut(s) 1979
StyD4I CCNGG 4 cut(s) 315, 1496, 1830, 2286
StyI CCWWGG 4 cut(s) 1980, 2171, 2490, 2521
TaaI ACNGT 7 cut(s) 263, 1435, 1448, 1624, 1784, 2098, 2276
TaiI ACGT 5 cut(s) 241, 1349, 1889, 2023, 2028
TaqI TCGA 7 cut(s) 62, 234, 706, 904, 1675, 2259, 2593
TatI WGTACW 2 cut(s) 2271, 2976
TauI GCSGC 1 cut(s) 1512
TfiI GAWTC 6 cut(s) 410, 1234, 1695, 1702, 2183, 2916
TscAI CASTG 5 cut(s) 1080, 1453, 2002, 2281, 2946
TseFI GTSAC 3 cut(s) 2296, 2390, 2627
TseI GCWGC 7 cut(s) 118, 271, 371, 1710, 2178, 2744, 2825
Tsp45I GTSAC 3 cut(s) 2296, 2390, 2627
TspDTI ATGAA 6 cut(s) 43, 281, 749, 1251, 2061, 2657
TspGWI ACGGA 1 cut(s) 1009
TspRI CASTG 5 cut(s) 1080, 1453, 2002, 2281, 2946
Vha464I CTTAAG 3 cut(s) 215, 1238, 1774
VpaK11BI GGWCC 1 cut(s) 2168
VspI ATTAAT 1 cut(s) 1328
XapI RAATTY 6 cut(s) 50, 530, 982, 1276, 1656, 2049
XceI RCATGY 4 cut(s) 343, 1408, 1990, 2304
XcmI CCANNNNNNNNNTGG 1 cut(s) 2344
XhoI CTCGAG 2 cut(s) 705, 2258
XmnI GAANNNNTTC 1 cut(s) 2679
Zsp2I ATGCAT 1 cut(s) 1427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.