pycom11g20010

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0011026
Physical Location & Seq
Reverse (-)
22564004 .. 22567057
3054 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g20010.1

Sequence Viewer

Length: 2922 bp
ATGCAAGAAATCGTGGGTGCTATCATTGAAGTTAGTAGGTGTGTCGGCAACTTCACGTTGTCGCTGCTTTCCACCCTGAGAAAGTTCCAGAAAAACATCCAAACTTTGAGGTATGAGCGGCAGAAGCTCTCTCGCTGCAAGTATGGAAAAATAGAAGAGGTCAATTTATCAAGATTAGAAGGGAAGCAGCCCCCTTGGCAAGTTATGGATTGGCTTGAAAGGGTGGAGAAGATCGAACGTGAGGTGGAAGGGATTGAACATGAATCCAGGCTACTTTTGGAGGCAGATCCCATTGATGCTCCCTGTGGCCTGGGGTGTATGCTTGACTCTAACATGCGCCGCAAGTACCAACTTAGCAAAGCTGCAGCAAAGAAGCGTGATGAGGTGAGAAAACTTACTGAGGAGTCATGCAATCTTCCACCTATGGAAGATAGGAAGCCAAGTGATATAGCGGTTGAGCATATTCTGGCACCATCTCTTGTTGGTCAGAAAGCACCAGAGATCCTGAAGCAGCTTATGGTACTTTTGGCTGTTAAAGAGATCACAAGGATTTCTGTTTATGGAATGGGCGGTTCTGGGAAGACTACGCTAGTGAAAACCTTGAATAATCAGCTTGAATCTTCTGGCAGGGAGTTATTTGATATGGTCATTTGGGTCCCTGTGTCAAATGACCTGGATATGAAAAAGGTTCAATCACGAATTGCTGAGAGGTTGAATTTAGCACTGAATGCAGAAGAGAGCATCGAACGTAGAGCTAGCAAACTGCAGCAAGTATTGAAGTCCGGAAAGAGGTTCCTGTTCATTCTGGATGACGTTTGGGAGAAAATTGATTTGCACAGCGTGGGGATACCACAAGGCGATGGCCAAGCAAATTGTAAGATCGTTTTGACAACACGAAATCTTGACGTGTGCAGGGAAATGATGACCGATAAAGCACTAAAGATGGAACTTCTGACTGAAGAAGAGTCCTGGAGTTTATTTGCTCAGAATGCAGGAAATGTGGTGGAGTTAGAAGACATAAATCCTCTAGCTAAAGAAATTGCGAGGGAATGTGGTGGTTTGCCTCTGGCTATCGAGACTATGGGGAAGTCCATGAGAGACAAAACAATGATACAGCTATGGAGGAATGCACTCCGCCAACTGAAGCTTTCCGATCCACACTTCGGGAGCTTGGACAAGGTGTATCTGCGGTTGCAATTGAGCTACAATGCACTGCCAAGTAAGATTTATAAGTCGTGCTTCTTATCTTGTTCGCTGTTCCCGGAAAATTTCTCAATTCAGATAAGGGAACTAATATTGTTATGGTTATCTGATGGAATTATTGGGGAACGTCAAACTTTAGAGGAGTCCATCAATGATGGGCATGCTAAACTTGAATATTTGAAGGATTGTTGCATGTTAGAACAGGGTGAAGGCATTGGCACCGTAAAGATGCACAGTATATTGAGGGAGGTTTCCATTTGGATATCCTCAAATAAGAAAGAAACAGGGTTTTTCAGCAGCCCAGCGCAAGGAATGCTAAAGCTGAAAAAATCGGTCAGAAGAGCTTCGTTTATGAGCAATTCCATCAAGAGTTTGCCAGCTCAATTGCTTGGGGGATCCGACCTAACTGTGTTGTTCCTGCATTGTAATCTTTTTAATAGAATCCCTGATGGTTTCTTTCGAGAACATAGAGTGCTAAGATTCTTGAATTTAAGTAGCACTCTAATAACTTCCCTGCCTTCTTCTATTCTTCAGCTGGGGGAGCTAAATACTCTTCTCTTAAGAGACTGTCGTGCTCTAGAAATCCTACCCCCACTCGGAGCTCTTTATAAACTTCAAGTGCTCGATCTCTATGGAACTCGAATAAGAAAGCTTCCGAAAGACATGGGAAAGCTGATTCATCTGAGAGATCTCAATTTGTCACACACTCGCTTTTTACAAGTCATTGCATATGGAAGCATGTTGGGATTAATTAGTCTTGAAGTCTTAGACATGTCTTTCAGTGGTTATAACTGGGATGTGAAGCGTAATGCAGGAAGAGGAGCAGCATTTGATGAGTTATTATCCTTGCGGCGACTTTCTGTTGTTCACATAAGGCTAAATACAGTTGATTGCGTTGCCTTAGACTCTGCCGGTCCTTGGTTTGGAAGGTTGAAGGAGTACACCATACAGATTGGTCCAAATTCAATCTATCTACCGACTCAGCATGAGAAAAGAGTCATCCTTCGAGGGGTTGACCTCCTGCAACGAGGCTTAGAGGGGCTATTGTGCACAGCAAGTGCCCTGGAGCTGTTCACATGTGGAGGTATAAGTAGTTTATCTGATATAATTACTAACAGGAGTGTGTGTGGCTTGTCAAATTTGAAGTCGCTCACCATATCAAATTGTGACTGCATAACCACATTGTTAGTTGGCGAAAAAACAATTCGGAGCACATTGCCAAATTTGGAGCACTTAACTCTGAGTCATCTGGATAACTTAGCAACCTTGTTGGACAATATAGTCCCCAGAAGATCCCTTGGAAACTTAAAGACCATCAAGGTGGAGGGATGTCGACTTCTAAAGAACCTCATATCTTTTTCTTTACTTCGACTGGTTTCAAACCTCGAAGAAATCAAGGTGAGTGACTGCAGGAGGATGAAGCAGGTTATCGCAAGAGATTTCTATGAAAAGATTCCAAAGTTGAAAACCATAGAAGTCAGGGATATGGAAAGCTTAAGTAGTATCTGTTCAAGGGGAGCGGAACTGTCAGCTCTAGAGAGGATTGTGGTGAGCAACTGCCCCAGGGTGGTGAACCTTCCCTTCACAGCCCCTGATGCATTGACTATTAAAGAGATCAGAGGAGAATTAAACTGGTGGACAGGTCTTAAATGGCAAAATCGTGCAGATAAGATCAGTCTTCAGCAACGATTCCAGGCGTGCGCAGACACCGCAGTGTAG

Protein Analysis

974

Amino Acids

109.79

Weight (kDa)

8.87

Isoelectric Point (pI)

41.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 175 - 332 9.8e-41 NB-ARC domain
AAA_22 PF13401 184 - 272 7.2e-06 AAA domain
LRR_14 PF23598 514 - 689 1.2e-10 Leucine-rich repeat region
LRR_RPS2 PF23247 755 - 872 9e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 829 - 926 2.2e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011026)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27190 AT4G27220 AT4G27220
fragaria_vesca FvH4_3g21750
malus_domestica MD11G1227400.v1.1 MD11G1227500.v1.1
prunus_persica Prupe.4G201800_v2.0.a1
pyrus_communis pycom11g20010
rosa_chinensis RchiOBHm_Chr5g0037231
rosa_multiflora Rmu_sc0005002.1_g000030
rosa_roxburghii Rroxscaffold_1G00043740
rosa_rugosa Rorug05G0163900 Rorug05G0163900
rosa_samantha Rh5AG253500 Rh5BG255500 Rh5CG288200 Rh5CG288300 Rh5DG263900
rosa_wichuraiana Rw5G023420 Rw5G023450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1230, 1812, 1992
AasI GACNNNNNNGTC 1 cut(s) 2483
Acc16I TGCGCA 1 cut(s) 2905
Acc36I ACCTGC 1 cut(s) 2617
AccB1I GGYRCC 2 cut(s) 469, 1421
AccBSI CCGCTC 2 cut(s) 118, 2723
AccI GTMKAC 1 cut(s) 2536
AccIII TCCGGA 1 cut(s) 782
AciI CCGC 9 cut(s) 118, 340, 452, 570, 1135, 1189, 2053, 2723, 2913
AclWI GGATC 6 cut(s) 281, 496, 1148, 1593, 1606, 2490
AcoI YGGCCR 1 cut(s) 862
AcsI RAATTY 6 cut(s) 715, 1267, 1690, 2164, 2341, 2425
AcuI CTGAAG 5 cut(s) 527, 978, 1163, 1718, 2867
AdeI CACNNNGTG 1 cut(s) 841
AfaI GTAC 3 cut(s) 347, 522, 2144
AfiI CCNNNNNNNGG 8 cut(s) 789, 1163, 1511, 1799, 2121, 2126, 2212, 2770
AflII CTTAAG 2 cut(s) 1762, 2698
AflIII ACRYGT 3 cut(s) 906, 1974, 2279
AjiI CACGTC 1 cut(s) 907
AjnI CCWGG 7 cut(s) 266, 309, 672, 968, 2265, 2765, 2895
AleI CACNNNNGTG 1 cut(s) 2915
Alw21I GWGCWC 6 cut(s) 1780, 1807, 1827, 2255, 2417, 2436
Alw26I GTCTC 3 cut(s) 1070, 1092, 1761
Alw44I GTGCAC 1 cut(s) 2251
AlwI GGATC 6 cut(s) 281, 496, 1148, 1593, 1606, 2490
AlwNI CAGNNNCTG 1 cut(s) 2795
Aor13HI TCCGGA 1 cut(s) 782
AoxI GGCC 2 cut(s) 307, 862
ApaLI GTGCAC 1 cut(s) 2251
ApeKI GCWGC 9 cut(s) 64, 135, 187, 362, 365, 511, 766, 1500, 2027
ApoI RAATTY 6 cut(s) 715, 1267, 1690, 2164, 2341, 2425
ArsI GACNNNNNNTTYG 2 cut(s) 2333, 2365
AseI ATTAAT 1 cut(s) 1952
Asp700I GAANNNNTTC 2 cut(s) 1546, 2655
AspLEI GCGC 3 cut(s) 339, 1510, 2906
AspS9I GGNCC 3 cut(s) 655, 2117, 2159
AsuC2I CCSGG 1 cut(s) 1262
AsuHPI GGTGA 6 cut(s) 397, 1421, 2347, 2614, 2764, 2785
AsuNHI GCTAGC 1 cut(s) 755
AvaII GGWCC 3 cut(s) 655, 2117, 2159
BaeGI GKGCMC 2 cut(s) 2255, 2266
BalI TGGCCA 1 cut(s) 864
BamHI GGATCC 1 cut(s) 1598
BanI GGYRCC 2 cut(s) 469, 1421
BanII GRGCYC 1 cut(s) 1807
BbsI GAAGAC 3 cut(s) 587, 1020, 2873
Bbv12I GWGCWC 6 cut(s) 1780, 1807, 1827, 2255, 2417, 2436
BbvI GCAGC 9 cut(s) 51, 122, 199, 349, 377, 523, 778, 1512, 2039
BccI CCATC 9 cut(s) 481, 854, 937, 1307, 1352, 1358, 1574, 1646, 2525
BciT130I CCWGG 7 cut(s) 268, 311, 674, 970, 2267, 2767, 2897
BciVI GTATCC 1 cut(s) 840
BcnI CCSGG 1 cut(s) 1262
BcoDI GTCTC 3 cut(s) 1070, 1092, 1761
BfaI CTAG 5 cut(s) 590, 756, 1028, 1781, 2738
BfmI CTRYAG 3 cut(s) 363, 764, 2611
BfrI CTTAAG 2 cut(s) 1762, 2698
BfuAI ACCTGC 1 cut(s) 2617
BfuI GTATCC 1 cut(s) 840
BglI GCCNNNNNGGC 1 cut(s) 196
BglII AGATCT 1 cut(s) 1891
Bme1390I CCNGG 8 cut(s) 268, 311, 674, 970, 1262, 2267, 2767, 2897
Bme18I GGWCC 3 cut(s) 655, 2117, 2159
BmgBI CACGTC 1 cut(s) 907
BmgT120I GGNCC 3 cut(s) 655, 2117, 2159
BmiI GGNNCC 6 cut(s) 471, 656, 657, 794, 1423, 1600
BmrFI CCNGG 8 cut(s) 268, 311, 674, 970, 1262, 2267, 2767, 2897
BmrI ACTGGG 1 cut(s) 2005
BmsI GCATC 4 cut(s) 286, 750, 1422, 2788
BmtI GCTAGC 1 cut(s) 759
BmuI ACTGGG 1 cut(s) 2005
BpiI GAAGAC 3 cut(s) 587, 1020, 2873
BpmI CTGGAG 2 cut(s) 991, 2288
BpuMI CCSGG 1 cut(s) 1262
BsaJI CCNNGG 7 cut(s) 194, 310, 2120, 2265, 2500, 2765, 2766
BsaWI WCCGGW 1 cut(s) 782
BsaXI ACNNNNNCTCC 2 cut(s) 2314, 2344
Bsc4I CCNNNNNNNGG 8 cut(s) 789, 1163, 1511, 1799, 2121, 2126, 2212, 2770
Bse118I RCCGGY 1 cut(s) 2114
Bse1I ACTGG 3 cut(s) 2000, 2580, 2840
Bse3DI GCAATG 2 cut(s) 1926, 2417
BseAI TCCGGA 1 cut(s) 782
BseBI CCWGG 7 cut(s) 268, 311, 674, 970, 2267, 2767, 2897
BseDI CCNNGG 7 cut(s) 194, 310, 2120, 2265, 2500, 2765, 2766
BseGI GGATG 6 cut(s) 96, 814, 2005, 2202, 2537, 2625
BseLI CCNNNNNNNGG 8 cut(s) 789, 1163, 1511, 1799, 2121, 2126, 2212, 2770
BseMI GCAATG 2 cut(s) 1926, 2417
BseMII CTCAG 7 cut(s) 68, 390, 696, 998, 1877, 2198, 2435
BseNI ACTGG 3 cut(s) 2000, 2580, 2840
BseRI GAGGAG 4 cut(s) 416, 1358, 2037, 2838
BseSI GKGCMC 2 cut(s) 2255, 2266
BseXI GCAGC 9 cut(s) 51, 122, 199, 349, 377, 523, 778, 1512, 2039
BseYI CCCAGC 2 cut(s) 1504, 1738
BsgI GTGCAG 2 cut(s) 931, 2886
BshFI GGCC 2 cut(s) 309, 864
BshNI GGYRCC 2 cut(s) 469, 1421
BsiHKAI GWGCWC 6 cut(s) 1780, 1807, 1827, 2255, 2417, 2436
BsiSI CCGG 3 cut(s) 783, 1262, 2115
BslFI GGGAC 2 cut(s) 641, 2471
BslI CCNNNNNNNGG 8 cut(s) 789, 1163, 1511, 1799, 2121, 2126, 2212, 2770
BsmAI GTCTC 3 cut(s) 1070, 1092, 1761
BsmFI GGGAC 2 cut(s) 641, 2471
BsmI GAATGC 4 cut(s) 733, 994, 1132, 1521
BsnI GGCC 2 cut(s) 309, 864
Bsp1286I GDGCHC 7 cut(s) 1780, 1807, 1827, 2255, 2266, 2417, 2436
Bsp13I TCCGGA 1 cut(s) 782
BspACI CCGC 9 cut(s) 118, 340, 452, 570, 1135, 1189, 2053, 2723, 2913
BspANI GGCC 2 cut(s) 309, 864
BspCNI CTCAG 7 cut(s) 69, 391, 697, 997, 1878, 2197, 2436
BspEI TCCGGA 1 cut(s) 782
BspLI GGNNCC 6 cut(s) 471, 656, 657, 794, 1423, 1600
BspMAI CTGCAG 3 cut(s) 367, 768, 2615
BspMI ACCTGC 1 cut(s) 2617
BspOI GCTAGC 1 cut(s) 759
BspPI GGATC 6 cut(s) 281, 496, 1148, 1593, 1606, 2490
BspQI GCTCTTC 1 cut(s) 1537
BspT107I GGYRCC 2 cut(s) 469, 1421
BspTI CTTAAG 2 cut(s) 1762, 2698
BsrBI CCGCTC 2 cut(s) 118, 2723
BsrDI GCAATG 2 cut(s) 1926, 2417
BsrFI RCCGGY 1 cut(s) 2114
BsrI ACTGG 3 cut(s) 2000, 2580, 2840
BssAI RCCGGY 1 cut(s) 2114
BssECI CCNNGG 7 cut(s) 194, 310, 2120, 2265, 2500, 2765, 2766
BssT1I CCWWGG 3 cut(s) 194, 2120, 2500
Bst2UI CCWGG 7 cut(s) 268, 311, 674, 970, 2267, 2767, 2897
Bst4CI ACNGT 6 cut(s) 1426, 1439, 1612, 1772, 2089, 2730
Bst6I CTCTTC 6 cut(s) 150, 729, 957, 1537, 1761, 2014
BstAFI CTTAAG 2 cut(s) 1762, 2698
BstAPI GCANNNNNTGC 2 cut(s) 728, 1516
BstC8I GCNNGC 4 cut(s) 757, 1365, 1581, 2902
BstF5I GGATG 6 cut(s) 96, 814, 2005, 2202, 2537, 2625
BstHHI GCGC 3 cut(s) 339, 1510, 2906
BstMAI GTCTC 3 cut(s) 1070, 1092, 1761
BstMWI GCNNNNNNNGC 8 cut(s) 124, 196, 728, 989, 1516, 1744, 2798, 2912
BstNI CCWGG 7 cut(s) 268, 311, 674, 970, 2267, 2767, 2897
BstNSI RCATGY 6 cut(s) 337, 1367, 1399, 1945, 1978, 2283
BstSCI CCNGG 8 cut(s) 266, 309, 672, 968, 1260, 2265, 2765, 2895
BstSFI CTRYAG 3 cut(s) 363, 764, 2611
BstSLI GKGCMC 2 cut(s) 2255, 2266
BstV1I GCAGC 9 cut(s) 51, 122, 199, 349, 377, 523, 778, 1512, 2039
BstV2I GAAGAC 3 cut(s) 587, 1020, 2873
BstX2I RGATCY 5 cut(s) 286, 501, 1598, 1891, 2495
BstXI CCANNNNNNTGG 1 cut(s) 2524
BstYI RGATCY 5 cut(s) 286, 501, 1598, 1891, 2495
BsuI GTATCC 1 cut(s) 840
BsuRI GGCC 2 cut(s) 309, 864
BtgZI GCGATG 1 cut(s) 873
BtrI CACGTC 1 cut(s) 907
BtsCI GGATG 6 cut(s) 96, 814, 2005, 2202, 2537, 2625
BtsI GCAGTG 2 cut(s) 1211, 2922
BtsIMutI CAGTG 4 cut(s) 722, 1211, 1990, 2922
BveI ACCTGC 1 cut(s) 2617
Cac8I GCNNGC 4 cut(s) 757, 1365, 1581, 2902
CaiI CAGNNNCTG 1 cut(s) 2795
CfoI GCGC 3 cut(s) 339, 1510, 2906
Cfr10I RCCGGY 1 cut(s) 2114
Cfr13I GGNCC 3 cut(s) 655, 2117, 2159
Csp6I GTAC 3 cut(s) 346, 521, 2143
CviQI GTAC 3 cut(s) 346, 521, 2143
DraIII CACNNNGTG 1 cut(s) 841
DrdI GACNNNNNNGTC 1 cut(s) 2483
DseDI GACNNNNNNGTC 1 cut(s) 2483
EaeI YGGCCR 1 cut(s) 862
Eam1104I CTCTTC 6 cut(s) 150, 729, 957, 1537, 1761, 2014
EarI CTCTTC 6 cut(s) 150, 729, 957, 1537, 1761, 2014
EciI GGCGGA 1 cut(s) 1124
Ecl136II GAGCTC 1 cut(s) 1805
Eco130I CCWWGG 3 cut(s) 194, 2120, 2500
Eco24I GRGCYC 1 cut(s) 1807
Eco32I GATATC 1 cut(s) 1467
Eco47I GGWCC 3 cut(s) 655, 2117, 2159
Eco53kI GAGCTC 1 cut(s) 1805
Eco57I CTGAAG 5 cut(s) 527, 978, 1163, 1718, 2867
EcoICRI GAGCTC 1 cut(s) 1805
EcoO109I RGGNCCY 1 cut(s) 655
EcoRII CCWGG 7 cut(s) 266, 309, 672, 968, 2265, 2765, 2895
EcoRV GATATC 1 cut(s) 1467
EcoT14I CCWWGG 3 cut(s) 194, 2120, 2500
EcoT22I ATGCAT 1 cut(s) 2803
EcoT38I GRGCYC 1 cut(s) 1807
ErhI CCWWGG 3 cut(s) 194, 2120, 2500
FalI AAGNNNNNCTT 2 cut(s) 1223, 1255
FaqI GGGAC 2 cut(s) 641, 2471
FauNDI CATATG 1 cut(s) 1933
FblI GTMKAC 1 cut(s) 2536
FokI GGATG 6 cut(s) 83, 821, 2012, 2189, 2544, 2632
FriOI GRGCYC 1 cut(s) 1807
FspBI CTAG 5 cut(s) 590, 756, 1028, 1781, 2738
FspI TGCGCA 1 cut(s) 2905
GlaI GCGC 3 cut(s) 338, 1509, 2905
GsaI CCCAGC 2 cut(s) 1508, 1742
GsuI CTGGAG 2 cut(s) 991, 2288
HaeIII GGCC 2 cut(s) 309, 864
HapII CCGG 3 cut(s) 783, 1262, 2115
HhaI GCGC 3 cut(s) 339, 1510, 2906
Hin6I GCGC 3 cut(s) 337, 1508, 2904
HinP1I GCGC 3 cut(s) 337, 1508, 2904
HincII GTYRAC 2 cut(s) 2218, 2537
HindII GTYRAC 2 cut(s) 2218, 2537
HindIII AAGCTT 3 cut(s) 1145, 1853, 2695
HpaII CCGG 3 cut(s) 783, 1262, 2115
HphI GGTGA 6 cut(s) 397, 1421, 2347, 2614, 2764, 2785
Hpy166II GTNNAC 8 cut(s) 2071, 2145, 2218, 2253, 2277, 2537, 2776, 2841
Hpy8I GTNNAC 8 cut(s) 2071, 2145, 2218, 2253, 2277, 2537, 2776, 2841
HpyCH4III ACNGT 6 cut(s) 1426, 1439, 1612, 1772, 2089, 2730
HpyCH4IV ACGT 6 cut(s) 56, 238, 748, 813, 906, 1330
HpyF10VI GCNNNNNNNGC 8 cut(s) 124, 196, 728, 989, 1516, 1744, 2798, 2912
HpySE526I ACGT 6 cut(s) 56, 238, 748, 813, 906, 1330
HspAI GCGC 3 cut(s) 337, 1508, 2904
KflI GGGWCCC 1 cut(s) 655
Kpn2I TCCGGA 1 cut(s) 782
LguI GCTCTTC 1 cut(s) 1537
LmnI GCTCC 9 cut(s) 304, 1167, 1744, 1802, 2024, 2269, 2412, 2431, 2720
Lsp1109I GCAGC 9 cut(s) 51, 122, 199, 349, 377, 523, 778, 1512, 2039
LweI GCATC 4 cut(s) 286, 750, 1422, 2788
MaeI CTAG 5 cut(s) 590, 756, 1028, 1781, 2738
MaeII ACGT 6 cut(s) 56, 238, 748, 813, 906, 1330
MaeIII GTNAC 3 cut(s) 1902, 2369, 2606
MbiI CCGCTC 2 cut(s) 118, 2723
MfeI CAATTG 2 cut(s) 1196, 1586
MflI RGATCY 5 cut(s) 286, 501, 1598, 1891, 2495
MhlI GDGCHC 7 cut(s) 1780, 1807, 1827, 2255, 2266, 2417, 2436
MlsI TGGCCA 1 cut(s) 864
MluNI TGGCCA 1 cut(s) 864
MlyI GAGTC 8 cut(s) 320, 413, 974, 1355, 2102, 2176, 2208, 2455
MmeI TCCRAC 2 cut(s) 1626, 2454
Mox20I TGGCCA 1 cut(s) 864
Mph1103I ATGCAT 1 cut(s) 2803
MroI TCCGGA 1 cut(s) 782
MroXI GAANNNNTTC 2 cut(s) 1546, 2655
MscI TGGCCA 1 cut(s) 864
MslI CAYNNNNRTG 2 cut(s) 2522, 2915
Msp20I TGGCCA 1 cut(s) 864
MspA1I CMGCKG 1 cut(s) 1738
MspCI CTTAAG 2 cut(s) 1762, 2698
MspI CCGG 3 cut(s) 783, 1262, 2115
MspR9I CCNGG 8 cut(s) 268, 311, 674, 970, 1262, 2267, 2767, 2897
MunI CAATTG 2 cut(s) 1196, 1586
Mva1269I GAATGC 4 cut(s) 733, 994, 1132, 1521
MvaI CCWGG 7 cut(s) 268, 311, 674, 970, 2267, 2767, 2897
MwoI GCNNNNNNNGC 8 cut(s) 124, 196, 728, 989, 1516, 1744, 2798, 2912
NciI CCSGG 1 cut(s) 1262
NdeI CATATG 1 cut(s) 1933
NheI GCTAGC 1 cut(s) 755
NlaIV GGNNCC 6 cut(s) 471, 656, 657, 794, 1423, 1600
NmuCI GTSAC 3 cut(s) 1902, 2369, 2606
NsbI TGCGCA 1 cut(s) 2905
NsiI ATGCAT 1 cut(s) 2803
NspI RCATGY 6 cut(s) 337, 1367, 1399, 1945, 1978, 2283
OliI CACNNNNGTG 1 cut(s) 2915
PaeI GCATGC 1 cut(s) 1367
PasI CCCWGGG 1 cut(s) 2766
PciI ACATGT 2 cut(s) 1974, 2279
PciSI GCTCTTC 1 cut(s) 1537
PctI GAATGC 4 cut(s) 733, 994, 1132, 1521
PdmI GAANNNNTTC 2 cut(s) 1546, 2655
PfeI GAWTC 7 cut(s) 263, 617, 1644, 1683, 1879, 2656, 2892
PfoI TCCNGGA 2 cut(s) 968, 1260
PleI GAGTC 8 cut(s) 320, 412, 973, 1354, 2102, 2176, 2207, 2454
PpsI GAGTC 8 cut(s) 320, 412, 973, 1354, 2102, 2176, 2207, 2454
PpuMI RGGWCCY 1 cut(s) 655
PscI ACATGT 2 cut(s) 1974, 2279
PshBI ATTAAT 1 cut(s) 1952
PsiI TTATAA 3 cut(s) 1230, 1812, 1992
Psp124BI GAGCTC 1 cut(s) 1807
Psp5II RGGWCCY 1 cut(s) 655
Psp6I CCWGG 7 cut(s) 266, 309, 672, 968, 2265, 2765, 2895
PspFI CCCAGC 2 cut(s) 1504, 1738
PspGI CCWGG 7 cut(s) 266, 309, 672, 968, 2265, 2765, 2895
PspN4I GGNNCC 6 cut(s) 471, 656, 657, 794, 1423, 1600
PspPI GGNCC 3 cut(s) 655, 2117, 2159
PspPPI RGGWCCY 1 cut(s) 655
PsrI GAACNNNNNNTAC 2 cut(s) 2695, 2727
PstI CTGCAG 3 cut(s) 367, 768, 2615
PstNI CAGNNNCTG 1 cut(s) 2795
PsuI RGATCY 5 cut(s) 286, 501, 1598, 1891, 2495
PvuII CAGCTG 1 cut(s) 1738
RsaI GTAC 3 cut(s) 347, 522, 2144
RsaNI GTAC 3 cut(s) 346, 521, 2143
RseI CAYNNNNRTG 2 cut(s) 2522, 2915
SacI GAGCTC 1 cut(s) 1807
SalI GTCGAC 1 cut(s) 2535
SapI GCTCTTC 1 cut(s) 1537
Sau96I GGNCC 3 cut(s) 655, 2117, 2159
SchI GAGTC 8 cut(s) 320, 413, 974, 1355, 2102, 2176, 2208, 2455
ScrFI CCNGG 8 cut(s) 268, 311, 674, 970, 1262, 2267, 2767, 2897
SduI GDGCHC 7 cut(s) 1780, 1807, 1827, 2255, 2266, 2417, 2436
SfaNI GCATC 4 cut(s) 286, 750, 1422, 2788
SfcI CTRYAG 3 cut(s) 363, 764, 2611
SinI GGWCC 3 cut(s) 655, 2117, 2159
SmiMI CAYNNNNRTG 2 cut(s) 2522, 2915
SmlI CTYRAG 2 cut(s) 1762, 2698
SmoI CTYRAG 2 cut(s) 1762, 2698
SphI GCATGC 1 cut(s) 1367
SsiI CCGC 9 cut(s) 118, 340, 452, 570, 1135, 1189, 2053, 2723, 2913
SspI AATATT 2 cut(s) 1296, 1379
SspMI CTAG 5 cut(s) 590, 756, 1028, 1781, 2738
SstI GAGCTC 1 cut(s) 1807
StyD4I CCNGG 8 cut(s) 266, 309, 672, 968, 1260, 2265, 2765, 2895
StyI CCWWGG 3 cut(s) 194, 2120, 2500
TaaI ACNGT 6 cut(s) 1426, 1439, 1612, 1772, 2089, 2730
TaiI ACGT 6 cut(s) 59, 241, 751, 816, 909, 1333
TaqII GACCGA 2 cut(s) 941, 1525
TatI WGTACW 1 cut(s) 2142
TauI GCSGC 3 cut(s) 121, 342, 2056
TfiI GAWTC 7 cut(s) 263, 617, 1644, 1683, 1879, 2656, 2892
TscAI CASTG 4 cut(s) 729, 1218, 1990, 2922
TseFI GTSAC 3 cut(s) 1902, 2369, 2606
TseI GCWGC 9 cut(s) 64, 135, 187, 362, 365, 511, 766, 1500, 2027
Tsp45I GTSAC 3 cut(s) 1902, 2369, 2606
TspDTI ATGAA 6 cut(s) 276, 695, 790, 1871, 2636, 2664
TspRI CASTG 4 cut(s) 729, 1218, 1990, 2922
Vha464I CTTAAG 2 cut(s) 1762, 2698
VneI GTGCAC 1 cut(s) 2251
VpaK11BI GGWCC 3 cut(s) 655, 2117, 2159
VspI ATTAAT 1 cut(s) 1952
XapI RAATTY 6 cut(s) 715, 1267, 1690, 2164, 2341, 2425
XbaI TCTAGA 2 cut(s) 1780, 2737
XceI RCATGY 6 cut(s) 337, 1367, 1399, 1945, 1978, 2283
XcmI CCANNNNNNNNNTGG 1 cut(s) 274
XmiI GTMKAC 1 cut(s) 2536
XmnI GAANNNNTTC 2 cut(s) 1546, 2655
XspI CTAG 5 cut(s) 590, 756, 1028, 1781, 2738
Zsp2I ATGCAT 1 cut(s) 2803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.