Rw5G023450

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011026
Physical Location & Seq
Reverse (-)
33038792 .. 33042072
3281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G023450.1

Sequence Viewer

Length: 2970 bp
ATGGAAACTTTGGGTGCTTTCTTTGTTGAAGTTTGCAAGCCTGTAACCAAATTCATGTGGTCGAAGTTTGCCACCCTGAAAAAATTCCCAAAAAACATCCAAACTTTGAAGAAGGAGTTGCAAAGCCTCAGTTGCCGAAGGGATGAGATAACAGATGATATTGATATAGCAAAACTAGAAGGGAAGTTCCCAGCAAGGCAAGTTGTGGACTGGCTTAAGAAGGTTGAAGCCATTGAATGTGCTGTTGAGGAGGAGATTGACCGTAAAGTGCAGCCAGTTTTGCAAGAAGTTCATATTAATGCTTCTGTTAATGGCCAGGGATGTCTTTTTGACTCTAACATGCACCAGAGGTACCATCTTAGCAAAACTGCAGCAAAGAAGTGTGATGAGGTAAAACAATTGATTACTGAATCATACAATTTTCCTACCATGTGGGATGCTTTGAGGCTTCGTGATACACCCATTCAGCATATTCCGGCACCATCTCTTGTTGGTCAGAAAGCACAAGAGAAGCTAAAGCAGCTGATGGAATTTCTGGCTGATAATAAGATCAGAAGGATTGCTGTCTATGGAATGGGAGGTTCTGGGAAGACTACGCTTGTAAAAACCTTGAACAATCAGCTCCAGTCTTCTGCTTCAAAGTTATGTGATATGGTCATCTGGATCCCAGTCTCAAATGACTTGGATGTGAGTAAGGTTCAATCTCGAGTTGCTGAGAGATTGAACTTAGCAATGAATGCAGAACAGAGCATAGAGTGTAGGGCTTATAGATTACATCAAGCATTAGAGTCAGGAAAAAGGTATATGTTAATAATTTCATCTCCTTATGTGCAACGGTTCCTCCTTATTCTTGATGATGTTTGGGAGAAAATTGACTTGGACACTGTGGGGATACCACAAGGTGATGACCAAGCAAACTGCAAGATTGTAATGACAACTCGATCTCTTGCCGTGTGTAGGGAAATGATGACTGATAAGGAACTAAAGATGGAACTTCTGAATGAGGAAGAGGCTTGGAATTTATTTGCACGGAATGCAGGAATTGTAGTGGAGTCAGAACACATAAATCCTCTAGCAAGAGCAATTGCTAGCGAATGCGGTGGTTTACCACTGGCAATTGAGACTATGGGGAAGTCAATGAGGGACAAAACAATGATACAGCTATGGAAGAATGCAATATGCCAGTTGAAGCATTCAGCATCACATTATGGGAGCTTTGACAAGGTGCATCTGCGACTGGAATTGAGTTATAACTCTTTACCAAGTAAGATTCTTAAGAGGTGTTTCTTGTTTTGTTCATTGTACCCAGAAAATTTCTTAATTACTGCAAGAGAACTAATATGTGGCTGGATAGCGGATGGATTAATTATTGAGCATCCAACGTTAGAGGAGTCATTCAATGATGGGATTGCAAAAATTGAATATTTGAAAGACTCTTGCATGCTAGAACAGGGTGAATGCATTGGAACAGTAAAGATGCACAGTGTATTGAGGGAAGTAGCCTTGTGGATATCCTCAAATAGAAAAGAAACCGGGTTTTTCAGCAGCTCATTGCTGGGAATGCAAGAAAAGTTAGAGAGTTCTATCAGAGGAGTTTCATTTATGAAGAACTCTATAACCAGTTTGCCAAGTCGGTTCTCAGGGTGCTCCAACCTTACTGTATTGTTCCTCCAATGTAATCCTCTGTATAAAATTCCTGATGGTTTCTTTCGAGAACTTAGAGCCCTAAGATTCTTGAATCTAAGCAGCACTCAAATATCCTCCTTGCCTTCTTCTCTTCTTCAACTAGCAGAGCTACACACTCTTCTCTTAAGAGACTGTTCTTCCCTAGAAAATCTGCCTCAAATTGGAGCCCTTTATAAACTCCAGGTGCTTGATCTCTCTGGCACACACATAAGAGAACTTCCAAAAGACATGGGAACATTAACTCATTTGAGAGATCTGAACTTGTCGCGCACAAACCACTTAGAAAGTATCATAGCTGGAAGCATCTCAGGATTATACAGTCTTGAGGTCTTGGACATGTCTTCCAGTGCTTATAAGTGGGATTTGGAACGTAACGTAGAAGGAGCACCATTTGATGAAATTCTATCCTTACAGGGACTTTCAGTTCTTCACATAAGGCTAGACACAGTTAATTGTGTTGCTTTAGACTCGGATGGCTCTTGGTTTGGAAGATTGAAAGAGTTTTCTATTTGGATTGGTCCAAGGAGCTGCGATTCAAGCTATTTACCAACTCAGCATGATGAGAAAAGGGTCATACTTAGAGGGGTTGACCTCTTGCAAAGTGGCCTTGAGGGGTTATTGTACAGTGCAAGTTCCCTGGATCTGGTCACATGTGGAGGTATAAGTAGTTTATCTGATCTAATTTCCAGCAGGAAACTGGGTGGTCTGCCAAACTTGAAGTTTCTCACCATCTCAAATTGTGACTGCATTACTAGTTTGTTAATTGGCGAAGAAACTCTTGGGAGCACATTGTCAAATCTGGAGCATTTGACGCTCAATTGCCTAAACAACTTGGAAACTATGGTGGACAGGATAGTCCCTAGAGGATGCCTTGGCAAATTAAAGATGATTGAAGTGATGGGATGCCGAAGACTAAAGACTCTCATATCCTTCACTTTACTTCGACGGGTTCAAAATGTTGAAGAAATCAAGGTGAGTGATTGCAGAAGGATGAAGAAAATTATTGTAAACATCAATTTGTGCGAAACTCTTCCAAAGCTGAAATACATAGAACTAAGGAATATGGACAGCTTAACAGCTATTTGTTCAAAGGCCGCAAAGTGGTCTGCTCTGGAAAGGATTGAAGTGAGCGATTGCCCCAAGCTAGAGAAGCTTCCACTCAGAGCCCAGAATGCAGCAAATCTTAAAGAAATTAGAGGGGACTTGAGTTGGTGGAATGGTCTTAGATGGGAAAATGATGGAGATAAGATCAGTCTTGAGCAACGATTCCAAGCGTGTGCTTGA

Protein Analysis

989

Amino Acids

111.5

Weight (kDa)

7.19

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 169 - 345 2.6e-34 NB-ARC domain
LRR_14 PF23598 529 - 746 2.5e-18 Leucine-rich repeat region
LRR_8 PF13855 550 - 607 1.3e-08 Leucine rich repeat
LRR_8 PF13855 621 - 677 4.7e-07 Leucine rich repeat
LRR_RPS2 PF23247 848 - 947 5.8e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011026)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27190 AT4G27220 AT4G27220
fragaria_vesca FvH4_3g21750
malus_domestica MD11G1227400.v1.1 MD11G1227500.v1.1
prunus_persica Prupe.4G201800_v2.0.a1
pyrus_communis pycom11g20010
rosa_chinensis RchiOBHm_Chr5g0037231
rosa_multiflora Rmu_sc0005002.1_g000030
rosa_roxburghii Rroxscaffold_1G00043740
rosa_rugosa Rorug05G0163900 Rorug05G0163900
rosa_samantha Rh5AG253500 Rh5BG255500 Rh5CG288200 Rh5CG288300 Rh5DG263900
rosa_wichuraiana Rw5G023420 Rw5G023450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1251, 1860, 2040
AasI GACNNNNNNGTC 1 cut(s) 2540
Acc65I GGTACC 1 cut(s) 351
AccB1I GGYRCC 2 cut(s) 351, 478
AccII CGCG 1 cut(s) 1954
AciI CCGC 3 cut(s) 1098, 1355, 2781
AclI AACGTT 1 cut(s) 1382
AclWI GGATC 3 cut(s) 658, 671, 2334
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 7 cut(s) 50, 83, 530, 1018, 1312, 1692, 2085
AdeI CACNNNGTG 1 cut(s) 902
AfaI GTAC 3 cut(s) 353, 1304, 2309
AfiI CCNNNNNNNGG 4 cut(s) 957, 1847, 2329, 2787
AflII CTTAAG 3 cut(s) 215, 1274, 1810
AflIII ACRYGT 2 cut(s) 2022, 2336
AhlI ACTAGT 1 cut(s) 2438
AjnI CCWGG 3 cut(s) 315, 1866, 2322
AjuI GAANNNNNNNTTGG 4 cut(s) 860, 892, 2448, 2480
AloI GAACNNNNNNTCC 2 cut(s) 2094, 2126
Alw21I GWGCWC 3 cut(s) 1649, 2074, 2474
Alw26I GTCTC 3 cut(s) 676, 1115, 1809
AlwI GGATC 3 cut(s) 658, 671, 2334
Ama87I CYCGRG 1 cut(s) 705
AoxI GGCC 3 cut(s) 313, 2290, 2778
ApeKI GCWGC 7 cut(s) 271, 371, 520, 1545, 1746, 2214, 2861
ApoI RAATTY 7 cut(s) 50, 83, 530, 1018, 1312, 1692, 2085
AseI ATTAAT 2 cut(s) 297, 1364
Asp700I GAANNNNTTC 2 cut(s) 83, 2715
Asp718I GGTACC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 1956
AspS9I GGNCC 1 cut(s) 2204
AsuC2I CCSGG 1 cut(s) 1534
AsuHPI GGTGA 4 cut(s) 914, 1466, 2404, 2671
AsuNHI GCTAGC 1 cut(s) 1088
AvaI CYCGRG 1 cut(s) 705
AvaII GGWCC 1 cut(s) 2204
BalI TGGCCA 1 cut(s) 315
BamHI GGATCC 1 cut(s) 663
BanI GGYRCC 2 cut(s) 351, 478
BanII GRGCYC 3 cut(s) 1726, 1855, 2854
BbsI GAAGAC 4 cut(s) 596, 621, 2019, 2602
Bbv12I GWGCWC 3 cut(s) 1649, 2074, 2474
BbvI GCAGC 7 cut(s) 283, 383, 532, 1557, 1758, 2201, 2873
BceAI ACGGC 1 cut(s) 935
BciT130I CCWGG 3 cut(s) 317, 1868, 2324
BciVI GTATCC 1 cut(s) 885
BcnI CCSGG 1 cut(s) 1534
BcoDI GTCTC 3 cut(s) 676, 1115, 1809
BcuI ACTAGT 1 cut(s) 2438
BfmI CTRYAG 1 cut(s) 369
BfrI CTTAAG 3 cut(s) 215, 1274, 1810
BfuI GTATCC 1 cut(s) 885
BglII AGATCT 1 cut(s) 1939
BisI GCNGC 8 cut(s) 272, 372, 521, 1546, 1747, 2215, 2781, 2862
BlsI GCNGC 8 cut(s) 273, 373, 522, 1547, 1748, 2216, 2782, 2863
Bme1390I CCNGG 4 cut(s) 317, 1534, 1868, 2324
Bme18I GGWCC 1 cut(s) 2204
BmeT110I CYCGRG 1 cut(s) 705
BmgT120I GGNCC 1 cut(s) 2204
BmiI GGNNCC 5 cut(s) 353, 480, 665, 839, 1852
BmrFI CCNGG 4 cut(s) 317, 1534, 1868, 2324
BmrI ACTGGG 2 cut(s) 662, 2393
BmsI GCATC 8 cut(s) 427, 1208, 1237, 1384, 1467, 1998, 2543, 2579
BmtI GCTAGC 1 cut(s) 1092
BmuI ACTGGG 2 cut(s) 662, 2393
BpiI GAAGAC 4 cut(s) 596, 621, 2019, 2602
BpmI CTGGAG 3 cut(s) 608, 1850, 2507
BpuEI CTTGAG 4 cut(s) 2030, 2315, 2911, 2963
BpuMI CCSGG 1 cut(s) 1534
BsaJI CCNNGG 4 cut(s) 316, 2207, 2322, 2557
BsaXI ACNNNNNCTCC 2 cut(s) 825, 855
Bsc4I CCNNNNNNNGG 4 cut(s) 957, 1847, 2329, 2787
Bse3DI GCAATG 2 cut(s) 738, 1550
BseBI CCWGG 3 cut(s) 317, 1868, 2324
BseDI CCNNGG 4 cut(s) 316, 2207, 2322, 2557
BseLI CCNNNNNNNGG 4 cut(s) 957, 1847, 2329, 2787
BseMI GCAATG 2 cut(s) 738, 1550
BseMII CTCAG 6 cut(s) 142, 705, 1653, 2007, 2252, 2860
BseRI GAGGAG 4 cut(s) 263, 266, 1403, 1605
BseXI GCAGC 7 cut(s) 283, 383, 532, 1557, 1758, 2201, 2873
BseYI CCCAGC 2 cut(s) 190, 1555
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 1 cut(s) 1954
BshFI GGCC 3 cut(s) 315, 2292, 2780
BshNI GGYRCC 2 cut(s) 351, 478
BsiHKAI GWGCWC 3 cut(s) 1649, 2074, 2474
BsiHKCI CYCGRG 1 cut(s) 705
BsiSI CCGG 2 cut(s) 476, 1533
BslFI GGGAC 4 cut(s) 1157, 2115, 2528, 2900
BslI CCNNNNNNNGG 4 cut(s) 957, 1847, 2329, 2787
BsmAI GTCTC 3 cut(s) 676, 1115, 1809
BsmFI GGGAC 4 cut(s) 1157, 2115, 2528, 2900
BsmI GAATGC 8 cut(s) 742, 1039, 1100, 1177, 1192, 1463, 1566, 2863
BsnI GGCC 3 cut(s) 315, 2292, 2780
BsoBI CYCGRG 1 cut(s) 705
Bsp1286I GDGCHC 6 cut(s) 1649, 1726, 1855, 2074, 2474, 2854
Bsp1407I TGTACA 1 cut(s) 2307
Bsp143I GATC 8 cut(s) 549, 663, 941, 1876, 1939, 2326, 2362, 2934
BspACI CCGC 3 cut(s) 1098, 1355, 2781
BspANI GGCC 3 cut(s) 315, 2292, 2780
BspCNI CTCAG 6 cut(s) 141, 706, 1652, 2006, 2251, 2859
BspFNI CGCG 1 cut(s) 1954
BspLI GGNNCC 5 cut(s) 353, 480, 665, 839, 1852
BspMAI CTGCAG 1 cut(s) 373
BspOI GCTAGC 1 cut(s) 1092
BspPI GGATC 3 cut(s) 658, 671, 2334
BspT107I GGYRCC 2 cut(s) 351, 478
BspTI CTTAAG 3 cut(s) 215, 1274, 1810
BsrDI GCAATG 2 cut(s) 738, 1550
BsrGI TGTACA 1 cut(s) 2307
BssECI CCNNGG 4 cut(s) 316, 2207, 2322, 2557
BssMI GATC 8 cut(s) 549, 663, 941, 1876, 1939, 2326, 2362, 2934
BssT1I CCWWGG 2 cut(s) 2207, 2557
Bst2UI CCWGG 3 cut(s) 317, 1868, 2324
Bst6I CTCTTC 4 cut(s) 1002, 1782, 1809, 2721
BstAFI CTTAAG 3 cut(s) 215, 1274, 1810
BstAPI GCANNNNNTGC 2 cut(s) 737, 1034
BstAUI TGTACA 1 cut(s) 2307
BstC8I GCNNGC 3 cut(s) 38, 1090, 1442
BstFNI CGCG 1 cut(s) 1954
BstHHI GCGC 1 cut(s) 1956
BstKTI GATC 8 cut(s) 552, 666, 944, 1879, 1942, 2329, 2365, 2937
BstMAI GTCTC 3 cut(s) 676, 1115, 1809
BstMBI GATC 8 cut(s) 549, 663, 941, 1876, 1939, 2326, 2362, 2934
BstNI CCWGG 3 cut(s) 317, 1868, 2324
BstNSI RCATGY 4 cut(s) 343, 1444, 2026, 2340
BstSCI CCNGG 4 cut(s) 315, 1532, 1866, 2322
BstSFI CTRYAG 1 cut(s) 369
BstUI CGCG 1 cut(s) 1954
BstV1I GCAGC 7 cut(s) 283, 383, 532, 1557, 1758, 2201, 2873
BstV2I GAAGAC 4 cut(s) 596, 621, 2019, 2602
BstX2I RGATCY 3 cut(s) 663, 1939, 2326
BstYI RGATCY 3 cut(s) 663, 1939, 2326
BsuI GTATCC 1 cut(s) 885
BsuRI GGCC 3 cut(s) 315, 2292, 2780
BtsIMutI CAGTG 5 cut(s) 882, 1109, 1489, 2038, 2317
Cac8I GCNNGC 3 cut(s) 38, 1090, 1442
CfoI GCGC 1 cut(s) 1956
Cfr13I GGNCC 1 cut(s) 2204
CseI GACGC 1 cut(s) 2506
Csp6I GTAC 3 cut(s) 352, 1303, 2308
CviAII CATG 8 cut(s) 55, 340, 430, 1441, 1915, 2023, 2243, 2337
CviQI GTAC 3 cut(s) 352, 1303, 2308
DpnI GATC 8 cut(s) 551, 665, 943, 1878, 1941, 2328, 2364, 2936
DpnII GATC 8 cut(s) 549, 663, 941, 1876, 1939, 2326, 2362, 2934
DraIII CACNNNGTG 1 cut(s) 902
DrdI GACNNNNNNGTC 1 cut(s) 2540
DseDI GACNNNNNNGTC 1 cut(s) 2540
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 4 cut(s) 1002, 1782, 1809, 2721
EarI CTCTTC 4 cut(s) 1002, 1782, 1809, 2721
Eco130I CCWWGG 2 cut(s) 2207, 2557
Eco24I GRGCYC 3 cut(s) 1726, 1855, 2854
Eco32I GATATC 1 cut(s) 1512
Eco47I GGWCC 1 cut(s) 2204
Eco88I CYCGRG 1 cut(s) 705
EcoRII CCWGG 3 cut(s) 315, 1866, 2322
EcoRV GATATC 1 cut(s) 1512
EcoT14I CCWWGG 2 cut(s) 2207, 2557
EcoT22I ATGCAT 1 cut(s) 1463
EcoT38I GRGCYC 3 cut(s) 1726, 1855, 2854
ErhI CCWWGG 2 cut(s) 2207, 2557
FaeI CATG 8 cut(s) 58, 343, 433, 1444, 1918, 2026, 2246, 2340
FalI AAGNNNNNCTT 2 cut(s) 2448, 2480
FaqI GGGAC 4 cut(s) 1157, 2115, 2528, 2900
FatI CATG 8 cut(s) 54, 339, 429, 1440, 1914, 2022, 2242, 2336
Fnu4HI GCNGC 8 cut(s) 272, 372, 521, 1546, 1747, 2215, 2781, 2862
FriOI GRGCYC 3 cut(s) 1726, 1855, 2854
Fsp4HI GCNGC 8 cut(s) 272, 372, 521, 1546, 1747, 2215, 2781, 2862
GlaI GCGC 1 cut(s) 1955
GluI GCNGC 8 cut(s) 272, 372, 521, 1546, 1747, 2215, 2781, 2862
GsaI CCCAGC 2 cut(s) 194, 1559
GsuI CTGGAG 3 cut(s) 608, 1850, 2507
HaeIII GGCC 3 cut(s) 315, 2292, 2780
HapII CCGG 2 cut(s) 476, 1533
HgaI GACGC 1 cut(s) 2506
HhaI GCGC 1 cut(s) 1956
Hin1II CATG 8 cut(s) 58, 343, 433, 1444, 1918, 2026, 2246, 2340
Hin6I GCGC 1 cut(s) 1954
HinP1I GCGC 1 cut(s) 1954
HincII GTYRAC 1 cut(s) 2275
HindII GTYRAC 1 cut(s) 2275
HindIII AAGCTT 1 cut(s) 2837
HpaII CCGG 2 cut(s) 476, 1533
HphI GGTGA 4 cut(s) 914, 1466, 2404, 2671
Hpy166II GTNNAC 5 cut(s) 208, 1106, 2275, 2533, 2695
Hpy188I TCNGA 9 cut(s) 498, 554, 999, 1057, 1589, 1944, 2158, 2362, 2849
Hpy8I GTNNAC 5 cut(s) 208, 1106, 2275, 2533, 2695
Hpy99I CGWCG 1 cut(s) 2634
HpyAV CCTTC 9 cut(s) 106, 132, 173, 214, 549, 1779, 2060, 2626, 2667
HpyCH4IV ACGT 3 cut(s) 1382, 2056, 2061
HpySE526I ACGT 3 cut(s) 1382, 2056, 2061
Hsp92II CATG 8 cut(s) 58, 343, 433, 1444, 1918, 2026, 2246, 2340
HspAI GCGC 1 cut(s) 1954
KpnI GGTACC 1 cut(s) 355
Kzo9I GATC 8 cut(s) 549, 663, 941, 1876, 1939, 2326, 2362, 2934
LmnI GCTCC 8 cut(s) 627, 1212, 1652, 1850, 2069, 2211, 2469, 2488
Lsp1109I GCAGC 7 cut(s) 283, 383, 532, 1557, 1758, 2201, 2873
LweI GCATC 8 cut(s) 427, 1208, 1237, 1384, 1467, 1998, 2543, 2579
MaeII ACGT 3 cut(s) 1382, 2056, 2061
MaeIII GTNAC 4 cut(s) 43, 2057, 2332, 2426
MalI GATC 8 cut(s) 551, 665, 943, 1878, 1941, 2328, 2364, 2936
MboI GATC 8 cut(s) 549, 663, 941, 1876, 1939, 2326, 2362, 2934
MfeI CAATTG 4 cut(s) 398, 1083, 1116, 2503
MflI RGATCY 3 cut(s) 663, 1939, 2326
MhlI GDGCHC 6 cut(s) 1649, 1726, 1855, 2074, 2474, 2854
MlsI TGGCCA 1 cut(s) 315
MluNI TGGCCA 1 cut(s) 315
MlyI GAGTC 7 cut(s) 326, 797, 1061, 1400, 1427, 2147, 2599
MmeI TCCRAC 2 cut(s) 1403, 1674
Mox20I TGGCCA 1 cut(s) 315
Mph1103I ATGCAT 1 cut(s) 1463
MroXI GAANNNNTTC 2 cut(s) 83, 2715
MscI TGGCCA 1 cut(s) 315
MslI CAYNNNNRTG 1 cut(s) 297
Msp20I TGGCCA 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 523
MspCI CTTAAG 3 cut(s) 215, 1274, 1810
MspI CCGG 2 cut(s) 476, 1533
MspR9I CCNGG 4 cut(s) 317, 1534, 1868, 2324
MunI CAATTG 4 cut(s) 398, 1083, 1116, 2503
Mva1269I GAATGC 8 cut(s) 742, 1039, 1100, 1177, 1192, 1463, 1566, 2863
MvaI CCWGG 3 cut(s) 317, 1868, 2324
MvnI CGCG 1 cut(s) 1954
NciI CCSGG 1 cut(s) 1534
NdeII GATC 8 cut(s) 549, 663, 941, 1876, 1939, 2326, 2362, 2934
NheI GCTAGC 1 cut(s) 1088
NlaIII CATG 8 cut(s) 58, 343, 433, 1444, 1918, 2026, 2246, 2340
NlaIV GGNNCC 5 cut(s) 353, 480, 665, 839, 1852
NmuCI GTSAC 2 cut(s) 2332, 2426
NsiI ATGCAT 1 cut(s) 1463
NspI RCATGY 4 cut(s) 343, 1444, 2026, 2340
PaeI GCATGC 1 cut(s) 1444
PaeR7I CTCGAG 1 cut(s) 705
PciI ACATGT 2 cut(s) 2022, 2336
PctI GAATGC 8 cut(s) 742, 1039, 1100, 1177, 1192, 1463, 1566, 2863
PdmI GAANNNNTTC 2 cut(s) 83, 2715
PfeI GAWTC 6 cut(s) 410, 1270, 1731, 1738, 2219, 2952
PkrI GCNGC 8 cut(s) 273, 373, 522, 1547, 1748, 2216, 2782, 2863
PleI GAGTC 7 cut(s) 326, 796, 1060, 1399, 1427, 2147, 2599
PpsI GAGTC 7 cut(s) 326, 796, 1060, 1399, 1427, 2147, 2599
PscI ACATGT 2 cut(s) 2022, 2336
PshBI ATTAAT 2 cut(s) 297, 1364
PsiI TTATAA 3 cut(s) 1251, 1860, 2040
Psp1406I AACGTT 1 cut(s) 1382
Psp6I CCWGG 3 cut(s) 315, 1866, 2322
PspFI CCCAGC 2 cut(s) 190, 1555
PspGI CCWGG 3 cut(s) 315, 1866, 2322
PspN4I GGNNCC 5 cut(s) 353, 480, 665, 839, 1852
PspPI GGNCC 1 cut(s) 2204
PstI CTGCAG 1 cut(s) 373
PsuI RGATCY 3 cut(s) 663, 1939, 2326
PvuII CAGCTG 1 cut(s) 523
RsaI GTAC 3 cut(s) 353, 1304, 2309
RsaNI GTAC 3 cut(s) 352, 1303, 2308
RseI CAYNNNNRTG 1 cut(s) 297
SatI GCNGC 8 cut(s) 272, 372, 521, 1546, 1747, 2215, 2781, 2862
Sau3AI GATC 8 cut(s) 549, 663, 941, 1876, 1939, 2326, 2362, 2934
Sau96I GGNCC 1 cut(s) 2204
SchI GAGTC 7 cut(s) 326, 797, 1061, 1400, 1427, 2147, 2599
ScrFI CCNGG 4 cut(s) 317, 1534, 1868, 2324
SduI GDGCHC 6 cut(s) 1649, 1726, 1855, 2074, 2474, 2854
SfaNI GCATC 8 cut(s) 427, 1208, 1237, 1384, 1467, 1998, 2543, 2579
SfcI CTRYAG 1 cut(s) 369
Sfr274I CTCGAG 1 cut(s) 705
SinI GGWCC 1 cut(s) 2204
SlaI CTCGAG 1 cut(s) 705
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 8 cut(s) 215, 705, 1274, 1810, 2009, 2294, 2890, 2942
SmoI CTYRAG 8 cut(s) 215, 705, 1274, 1810, 2009, 2294, 2890, 2942
SpeI ACTAGT 1 cut(s) 2438
SphI GCATGC 1 cut(s) 1444
SsiI CCGC 3 cut(s) 1098, 1355, 2781
SspI AATATT 1 cut(s) 1424
StyD4I CCNGG 4 cut(s) 315, 1532, 1866, 2322
StyI CCWWGG 2 cut(s) 2207, 2557
TaiI ACGT 3 cut(s) 1385, 2059, 2064
TaqI TCGA 5 cut(s) 62, 706, 940, 1711, 2629
TatI WGTACW 1 cut(s) 2307
TauI GCSGC 1 cut(s) 2783
TfiI GAWTC 6 cut(s) 410, 1270, 1731, 1738, 2219, 2952
TscAI CASTG 5 cut(s) 889, 1116, 1489, 2038, 2317
TseFI GTSAC 2 cut(s) 2332, 2426
TseI GCWGC 7 cut(s) 271, 371, 520, 1545, 1746, 2214, 2861
Tsp45I GTSAC 2 cut(s) 2332, 2426
TspDTI ATGAA 9 cut(s) 43, 281, 749, 807, 1287, 1587, 1619, 2097, 2693
TspGWI ACGGA 1 cut(s) 1045
TspRI CASTG 5 cut(s) 889, 1116, 1489, 2038, 2317
Vha464I CTTAAG 3 cut(s) 215, 1274, 1810
VpaK11BI GGWCC 1 cut(s) 2204
VspI ATTAAT 2 cut(s) 297, 1364
XapI RAATTY 7 cut(s) 50, 83, 530, 1018, 1312, 1692, 2085
XceI RCATGY 4 cut(s) 343, 1444, 2026, 2340
XcmI CCANNNNNNNNNTGG 1 cut(s) 2380
XhoI CTCGAG 1 cut(s) 705
XmnI GAANNNNTTC 2 cut(s) 83, 2715
Zsp2I ATGCAT 1 cut(s) 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.