Rh5AG253500

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0011026
Physical Location & Seq
Reverse (-)
32533147 .. 32537822
4676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG253500.1

Sequence Viewer

Length: 2970 bp
ATGGAAACTTTGGGTGCTTTCTTTGTTGAAGTTTGCAAGTCTGTAACCAAATTCATGTGGTCGAAGTTTGCCACCCTGAAAAAATTCCCAAAAAACATCCAAACTTTGAAGAAGGAGTTGCAAAGCCTCAGTTGCCGAAGGGATGAGATAACAGATGATATTGATATAGCAAAACTAGAAGGGAAGTTCCCAGCAAGGCAAGTTGTGGACTGGCTTAAGAAGGTTGAAGCCATCGAATGTGCTGTTGAGGAGGAGATTGACCGTAAAGTGCAGCCAGTTTTGCAAGAAGTTCATATTAATGCTTCTGTTAATGGCCAGGGATGTCTTTTTGACTCTAACATGCACCAGAGGTACCATCTTAGCAAAACTGCAGCAAAGAAGTGTGATGAGGTAAAACAATTAATTACTGAATCATACAATTTTCCTACCATGTGGGATGCTTTGAGGCTTCGTGATACACCCATTCAGCATATTCCAGCACCATCTCTTGTTGGTCAGAAAGCACAAGAGAAGCTAAAGCAGCTGATGGAATTTCTGGCTGATAATAAGATCAGAAGGATTGCTGTCTATGGAATGGGAGGTTCTGGGAAGACTACGCTTGTAAAAACCTTGAACAATCAGCTCCAGTCTTCTGCTTTAAAGTTATGTGATATGGTCATCTGGATCCCAGTCTCAAATGACTTGGATGTGAGTAAGGTTCAATCTCGAGTTGCTGAGAGATTGAACTTAGCAATGAATGCAGAACAGAGCATAGAGTGTAGGGCTTGTAGATTACATCAAGCATTAAAGTCAGGAAAAAGGTTCCTCCTTATTCTTGATGATGTTTGGGAGAAAATTGACTTGGACACTGTGGGGATACCACAAGGTGATGACCAAGCAAACTGCAAGATCGTAATGACAACTCGATCTCTTGCCGTGTGTAGGGAAATGATGACTGATAAGGAACTAAAGATGGAACTTCTGAATGAGGAAGAGGCTTGGAATTTATTTGCACGGAATGCAGGAATTGTAGTGGAGTCAGAACACATAAATCCTTTAGCAAGAGCAATTGCTAGCGAATGCGGTGGTTTACCACTGGCAATTGAGACTATGGGGAAGTCAATGAGGGACAAAACAATGATACAGCTATGGAAGAATGCACTATGCCAGTTGAAGCATTCAGCATCACATTATGGGAGCTTTGACAAGGTGCATCTGCGACTGGAATTGAGTTATAACTCTTTACCAAGTAAGATTCTTAAGAGGTGTTTCTTGTTTTGTTCATTGTACCCAGAAAATTTCTTAATTACTGCAAGAGAACTAATATGTGGCTGGATAGCGGATGGATTAATTATTGAGCATCCAACGTTAGAGGAGTCATTCAATGATGGGATTGCAAAAATTGAATATTTGAAAGACTCTTGCATGCTAGAACAGGGTGAATGCATTGGAACAGTAAAGATGCACAATGTATTGAGGGAAGTAGCCTTGTGGATATCCTCAAATAGAAAAGAAACCAGGTTTTTCAGCAGCTCATTGCTGGGAATGCAAGAAAAGTTGGAGAGTTCTATCAGAGGAGTTTCATTTATGAAGAACTCTATAACCAGTTTGCCAAGTCGGTTCTCAGGGTGCTCCAACCTTACTGTATTGTTCCTCCAATGTAATCCTCTGTATAAAATTCCTGATGGTTTCTTTCGAGAACTTGGAGCCCTAAGATTCTTGAATCTAAGCAGCACTCAAATATCCTCCTTGCCTTCTTCGCTTCTTCAACTAGCAGAGCTACACACTCTTCTCTTAAGAGACTGTTCTTCCCTAGAAAATCTGCCTCAAATTGGAGCCCTTTATAAACTCCAGGTGCTTGATCTCTCTGGCACACACATAAGAGAACTTCCAAAAGACATGGGAACATTAACTCATTTGAGAGATCTAAACTTGTCGCGCACAAACCACTTAGAAAGTATCATAGCTGGAAGCATCTCAGGATTATACAGTCTTGAGGCCTTGGACATGTCTTCCAGTGCTTATAAGTGGGATTTGGAACGTAACGTAGAAGGAGCACCATTTGATGAAATTCTATCCTTACAGGGACTTTCAGTTCTTCACATAAGGCTAGACACAGTTAATTGTGTTGCTTTAGACTCGGATGGCTCTTGGTTTGGAAGATTGAAAGAGTTTTCTATTTGGATTGGTCCAAGGAGCTGCGATTCAAGCTATTTACCAACTCAGCATGATGAGAAAAGGGTCATACTTAGAGGGGTTGACCTCTTGCAAAGTGGCCTTGAGGGGTTATTGTACAGTGCAAGTTCCCTGGATCTGGTCACATGTGGAGGTATAAGTAGTTTATCTGCTCTAATTTCCAGCAGGAAACTGGGTGGCCTGCCAAACTTGAAGTTTCTCACCATCTCAAATTGTGACTGCATTACTAGTTTGTTAATTGGCGAAGAAACTCTTGGGACCACATTCTCAAATCTGGAGCATTTGACCCTCAATTGCCTAAACAACTTGGAAACTATGGTGGACAGGATAGTCCCTAGAGGATGCCTTGGCAAATTAAAGATCATTGAAGTGATGGGATGCCGAAGACTAAAGACTCTCATATCCTTCACTTTACTTCGACGGGTTCAAAATGTTGAAGAAATCAAGGTGAGTGATTGCAGAAGGATGAAGAAAATTATTGTAAACATCAATTTGTGCGAAACTCTTCCAAAGCTGAAATACATAGAACTAAGGAATATGGACAGCTTAACAGCTATTTGTTCAAAGGCCGCAAAGTGGTCTGCTCTGGAAAGGATTGAAGTGAGCGATTGCCCCAAGCTAGAGAAGCTTCCACTCAGAGCCCAGAATGCAGCAAATCTTAGAGAAATTAGAGGGGACTTGAGTTGGTGGAATGGTCTTAGATGGGAAAATGATGGAGATAAGATCAGTCTTGAGCAACGATTCCAAGAGTGTGCAGACTCCACACTGCTGTTAAAAGAGGATACAGAGGCTTGA

Protein Analysis

989

Amino Acids

111.39

Weight (kDa)

6.82

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 169 - 333 2.5e-38 NB-ARC domain
AAA_22 PF13401 187 - 275 1e-05 AAA domain
LRR_14 PF23598 517 - 850 1.6e-16 Leucine-rich repeat region
LRR_8 PF13855 538 - 595 2.4e-08 Leucine rich repeat
LRR_8 PF13855 609 - 665 3.2e-07 Leucine rich repeat
LRR_RPS2 PF23247 836 - 935 2.9e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011026)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27190 AT4G27220 AT4G27220
fragaria_vesca FvH4_3g21750
malus_domestica MD11G1227400.v1.1 MD11G1227500.v1.1
prunus_persica Prupe.4G201800_v2.0.a1
pyrus_communis pycom11g20010
rosa_chinensis RchiOBHm_Chr5g0037231
rosa_multiflora Rmu_sc0005002.1_g000030
rosa_roxburghii Rroxscaffold_1G00043740
rosa_rugosa Rorug05G0163900 Rorug05G0163900
rosa_samantha Rh5AG253500 Rh5BG255500 Rh5CG288200 Rh5CG288300 Rh5DG263900
rosa_wichuraiana Rw5G023420 Rw5G023450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1215, 1824, 2004
AasI GACNNNNNNGTC 1 cut(s) 2504
Acc65I GGTACC 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 351
AccII CGCG 1 cut(s) 1918
AciI CCGC 3 cut(s) 1062, 1319, 2745
AclI AACGTT 1 cut(s) 1346
AclWI GGATC 3 cut(s) 658, 671, 2298
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 7 cut(s) 50, 83, 530, 982, 1276, 1656, 2049
AdeI CACNNNGTG 1 cut(s) 866
AfaI GTAC 3 cut(s) 353, 1268, 2273
AfiI CCNNNNNNNGG 4 cut(s) 921, 1811, 2293, 2751
AflII CTTAAG 3 cut(s) 215, 1238, 1774
AflIII ACRYGT 2 cut(s) 1986, 2300
AhlI ACTAGT 1 cut(s) 2402
AjnI CCWGG 4 cut(s) 315, 1496, 1830, 2286
AjuI GAANNNNNNNTTGG 4 cut(s) 824, 856, 2412, 2444
AloI GAACNNNNNNTCC 2 cut(s) 2058, 2090
Alw21I GWGCWC 2 cut(s) 1613, 2038
Alw26I GTCTC 3 cut(s) 676, 1079, 1773
AlwI GGATC 3 cut(s) 658, 671, 2298
Ama87I CYCGRG 1 cut(s) 705
AoxI GGCC 5 cut(s) 313, 1977, 2254, 2353, 2742
ApeKI GCWGC 7 cut(s) 271, 371, 520, 1509, 1710, 2178, 2825
ApoI RAATTY 7 cut(s) 50, 83, 530, 982, 1276, 1656, 2049
AseI ATTAAT 3 cut(s) 297, 401, 1328
Asp700I GAANNNNTTC 2 cut(s) 83, 2679
Asp718I GGTACC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 1920
AspS9I GGNCC 2 cut(s) 2168, 2433
AsuHPI GGTGA 4 cut(s) 878, 1430, 2368, 2635
AsuNHI GCTAGC 1 cut(s) 1052
AvaI CYCGRG 1 cut(s) 705
AvaII GGWCC 2 cut(s) 2168, 2433
BalI TGGCCA 1 cut(s) 315
BamHI GGATCC 1 cut(s) 663
BanI GGYRCC 1 cut(s) 351
BanII GRGCYC 3 cut(s) 1690, 1819, 2818
BbsI GAAGAC 4 cut(s) 596, 621, 1983, 2566
Bbv12I GWGCWC 2 cut(s) 1613, 2038
BbvI GCAGC 7 cut(s) 283, 383, 532, 1521, 1722, 2165, 2837
BceAI ACGGC 1 cut(s) 899
BciT130I CCWGG 4 cut(s) 317, 1498, 1832, 2288
BciVI GTATCC 2 cut(s) 849, 2950
BcoDI GTCTC 3 cut(s) 676, 1079, 1773
BcuI ACTAGT 1 cut(s) 2402
BfaI CTAG 9 cut(s) 176, 1053, 1409, 1751, 1793, 2090, 2403, 2511, 2795
BfmI CTRYAG 1 cut(s) 369
BfrI CTTAAG 3 cut(s) 215, 1238, 1774
BfuI GTATCC 2 cut(s) 849, 2950
BglII AGATCT 1 cut(s) 1903
BisI GCNGC 8 cut(s) 272, 372, 521, 1510, 1711, 2179, 2745, 2826
BlsI GCNGC 8 cut(s) 273, 373, 522, 1511, 1712, 2180, 2746, 2827
Bme1390I CCNGG 4 cut(s) 317, 1498, 1832, 2288
Bme18I GGWCC 2 cut(s) 2168, 2433
BmeT110I CYCGRG 1 cut(s) 705
BmgT120I GGNCC 2 cut(s) 2168, 2433
BmiI GGNNCC 6 cut(s) 353, 665, 803, 1687, 1816, 2434
BmrFI CCNGG 4 cut(s) 317, 1498, 1832, 2288
BmrI ACTGGG 2 cut(s) 662, 2357
BmsI GCATC 8 cut(s) 427, 1172, 1201, 1348, 1431, 1962, 2507, 2543
BmtI GCTAGC 1 cut(s) 1056
BmuI ACTGGG 2 cut(s) 662, 2357
BpiI GAAGAC 4 cut(s) 596, 621, 1983, 2566
BpmI CTGGAG 3 cut(s) 608, 1814, 2471
BpuEI CTTGAG 4 cut(s) 1994, 2279, 2875, 2927
BsaJI CCNNGG 5 cut(s) 316, 1980, 2171, 2286, 2521
Bsc4I CCNNNNNNNGG 4 cut(s) 921, 1811, 2293, 2751
Bse3DI GCAATG 2 cut(s) 738, 1514
BseBI CCWGG 4 cut(s) 317, 1498, 1832, 2288
BseDI CCNNGG 5 cut(s) 316, 1980, 2171, 2286, 2521
BseLI CCNNNNNNNGG 4 cut(s) 921, 1811, 2293, 2751
BseMI GCAATG 2 cut(s) 738, 1514
BseMII CTCAG 6 cut(s) 142, 705, 1617, 1971, 2216, 2824
BseRI GAGGAG 4 cut(s) 263, 266, 1367, 1569
BseXI GCAGC 7 cut(s) 283, 383, 532, 1521, 1722, 2165, 2837
BseYI CCCAGC 2 cut(s) 190, 1519
BsgI GTGCAG 2 cut(s) 290, 2949
Bsh1236I CGCG 1 cut(s) 1918
BshFI GGCC 5 cut(s) 315, 1979, 2256, 2355, 2744
BshNI GGYRCC 1 cut(s) 351
BsiHKAI GWGCWC 2 cut(s) 1613, 2038
BsiHKCI CYCGRG 1 cut(s) 705
BslFI GGGAC 5 cut(s) 1121, 2079, 2446, 2492, 2864
BslI CCNNNNNNNGG 4 cut(s) 921, 1811, 2293, 2751
BsmAI GTCTC 3 cut(s) 676, 1079, 1773
BsmFI GGGAC 5 cut(s) 1121, 2079, 2446, 2492, 2864
BsmI GAATGC 8 cut(s) 742, 1003, 1064, 1141, 1156, 1427, 1530, 2827
BsnI GGCC 5 cut(s) 315, 1979, 2256, 2355, 2744
BsoBI CYCGRG 1 cut(s) 705
Bsp1286I GDGCHC 5 cut(s) 1613, 1690, 1819, 2038, 2818
Bsp1407I TGTACA 1 cut(s) 2271
Bsp143I GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2535, 2898
BspACI CCGC 3 cut(s) 1062, 1319, 2745
BspANI GGCC 5 cut(s) 315, 1979, 2256, 2355, 2744
BspCNI CTCAG 6 cut(s) 141, 706, 1616, 1970, 2215, 2823
BspFNI CGCG 1 cut(s) 1918
BspLI GGNNCC 6 cut(s) 353, 665, 803, 1687, 1816, 2434
BspMAI CTGCAG 1 cut(s) 373
BspOI GCTAGC 1 cut(s) 1056
BspPI GGATC 3 cut(s) 658, 671, 2298
BspT107I GGYRCC 1 cut(s) 351
BspTI CTTAAG 3 cut(s) 215, 1238, 1774
BsrDI GCAATG 2 cut(s) 738, 1514
BsrGI TGTACA 1 cut(s) 2271
BssECI CCNNGG 5 cut(s) 316, 1980, 2171, 2286, 2521
BssMI GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2535, 2898
BssT1I CCWWGG 3 cut(s) 1980, 2171, 2521
Bst2UI CCWGG 4 cut(s) 317, 1498, 1832, 2288
Bst4CI ACNGT 8 cut(s) 263, 850, 1435, 1624, 1784, 1970, 2098, 2276
Bst6I CTCTTC 3 cut(s) 966, 1773, 2685
BstAFI CTTAAG 3 cut(s) 215, 1238, 1774
BstAPI GCANNNNNTGC 2 cut(s) 737, 998
BstAUI TGTACA 1 cut(s) 2271
BstC8I GCNNGC 3 cut(s) 1054, 1406, 2357
BstFNI CGCG 1 cut(s) 1918
BstHHI GCGC 1 cut(s) 1920
BstKTI GATC 9 cut(s) 552, 666, 891, 908, 1843, 1906, 2293, 2538, 2901
BstMAI GTCTC 3 cut(s) 676, 1079, 1773
BstMBI GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2535, 2898
BstNI CCWGG 4 cut(s) 317, 1498, 1832, 2288
BstNSI RCATGY 4 cut(s) 343, 1408, 1990, 2304
BstSCI CCNGG 4 cut(s) 315, 1496, 1830, 2286
BstSFI CTRYAG 1 cut(s) 369
BstUI CGCG 1 cut(s) 1918
BstV1I GCAGC 7 cut(s) 283, 383, 532, 1521, 1722, 2165, 2837
BstV2I GAAGAC 4 cut(s) 596, 621, 1983, 2566
BstX2I RGATCY 3 cut(s) 663, 1903, 2290
BstYI RGATCY 3 cut(s) 663, 1903, 2290
BsuI GTATCC 2 cut(s) 849, 2950
BsuRI GGCC 5 cut(s) 315, 1979, 2256, 2355, 2744
BtsI GCAGTG 1 cut(s) 2939
BtsIMutI CAGTG 5 cut(s) 846, 1073, 2002, 2281, 2939
Cac8I GCNNGC 3 cut(s) 1054, 1406, 2357
CfoI GCGC 1 cut(s) 1920
Cfr13I GGNCC 2 cut(s) 2168, 2433
CsiI ACCWGGT 1 cut(s) 1496
Csp6I GTAC 3 cut(s) 352, 1267, 2272
CviAII CATG 8 cut(s) 55, 340, 430, 1405, 1879, 1987, 2207, 2301
CviQI GTAC 3 cut(s) 352, 1267, 2272
DpnI GATC 9 cut(s) 551, 665, 890, 907, 1842, 1905, 2292, 2537, 2900
DpnII GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2535, 2898
DraI TTTAAA 1 cut(s) 639
DraIII CACNNNGTG 1 cut(s) 866
DrdI GACNNNNNNGTC 1 cut(s) 2504
DseDI GACNNNNNNGTC 1 cut(s) 2504
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 3 cut(s) 966, 1773, 2685
EarI CTCTTC 3 cut(s) 966, 1773, 2685
Eco130I CCWWGG 3 cut(s) 1980, 2171, 2521
Eco147I AGGCCT 1 cut(s) 1979
Eco24I GRGCYC 3 cut(s) 1690, 1819, 2818
Eco32I GATATC 1 cut(s) 1476
Eco47I GGWCC 2 cut(s) 2168, 2433
Eco88I CYCGRG 1 cut(s) 705
EcoRII CCWGG 4 cut(s) 315, 1496, 1830, 2286
EcoRV GATATC 1 cut(s) 1476
EcoT14I CCWWGG 3 cut(s) 1980, 2171, 2521
EcoT22I ATGCAT 1 cut(s) 1427
EcoT38I GRGCYC 3 cut(s) 1690, 1819, 2818
ErhI CCWWGG 3 cut(s) 1980, 2171, 2521
FaeI CATG 8 cut(s) 58, 343, 433, 1408, 1882, 1990, 2210, 2304
FalI AAGNNNNNCTT 2 cut(s) 2412, 2444
FaqI GGGAC 5 cut(s) 1121, 2079, 2446, 2492, 2864
FatI CATG 8 cut(s) 54, 339, 429, 1404, 1878, 1986, 2206, 2300
Fnu4HI GCNGC 8 cut(s) 272, 372, 521, 1510, 1711, 2179, 2745, 2826
FriOI GRGCYC 3 cut(s) 1690, 1819, 2818
Fsp4HI GCNGC 8 cut(s) 272, 372, 521, 1510, 1711, 2179, 2745, 2826
FspBI CTAG 9 cut(s) 176, 1053, 1409, 1751, 1793, 2090, 2403, 2511, 2795
GlaI GCGC 1 cut(s) 1919
GluI GCNGC 8 cut(s) 272, 372, 521, 1510, 1711, 2179, 2745, 2826
GsaI CCCAGC 2 cut(s) 194, 1523
GsuI CTGGAG 3 cut(s) 608, 1814, 2471
HaeIII GGCC 5 cut(s) 315, 1979, 2256, 2355, 2744
HhaI GCGC 1 cut(s) 1920
Hin1II CATG 8 cut(s) 58, 343, 433, 1408, 1882, 1990, 2210, 2304
Hin6I GCGC 1 cut(s) 1918
HinP1I GCGC 1 cut(s) 1918
HincII GTYRAC 1 cut(s) 2239
HindII GTYRAC 1 cut(s) 2239
HindIII AAGCTT 1 cut(s) 2801
HphI GGTGA 4 cut(s) 878, 1430, 2368, 2635
Hpy166II GTNNAC 5 cut(s) 208, 1070, 2239, 2497, 2659
Hpy188I TCNGA 7 cut(s) 498, 554, 963, 1021, 1553, 2122, 2813
Hpy8I GTNNAC 5 cut(s) 208, 1070, 2239, 2497, 2659
Hpy99I CGWCG 1 cut(s) 2598
HpyAV CCTTC 9 cut(s) 106, 132, 173, 214, 549, 1743, 2024, 2590, 2631
HpyCH4III ACNGT 8 cut(s) 263, 850, 1435, 1624, 1784, 1970, 2098, 2276
HpyCH4IV ACGT 3 cut(s) 1346, 2020, 2025
HpySE526I ACGT 3 cut(s) 1346, 2020, 2025
Hsp92II CATG 8 cut(s) 58, 343, 433, 1408, 1882, 1990, 2210, 2304
HspAI GCGC 1 cut(s) 1918
KpnI GGTACC 1 cut(s) 355
Kzo9I GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2535, 2898
LmnI GCTCC 8 cut(s) 627, 1176, 1616, 1685, 1814, 2033, 2175, 2452
Lsp1109I GCAGC 7 cut(s) 283, 383, 532, 1521, 1722, 2165, 2837
LweI GCATC 8 cut(s) 427, 1172, 1201, 1348, 1431, 1962, 2507, 2543
MabI ACCWGGT 1 cut(s) 1496
MaeI CTAG 9 cut(s) 176, 1053, 1409, 1751, 1793, 2090, 2403, 2511, 2795
MaeII ACGT 3 cut(s) 1346, 2020, 2025
MaeIII GTNAC 4 cut(s) 43, 2021, 2296, 2390
MalI GATC 9 cut(s) 551, 665, 890, 907, 1842, 1905, 2292, 2537, 2900
MboI GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2535, 2898
MfeI CAATTG 3 cut(s) 1047, 1080, 2467
MflI RGATCY 3 cut(s) 663, 1903, 2290
MhlI GDGCHC 5 cut(s) 1613, 1690, 1819, 2038, 2818
MlsI TGGCCA 1 cut(s) 315
MluNI TGGCCA 1 cut(s) 315
MlyI GAGTC 7 cut(s) 326, 1025, 1364, 1391, 2111, 2563, 2927
MmeI TCCRAC 3 cut(s) 1367, 1518, 1638
Mox20I TGGCCA 1 cut(s) 315
Mph1103I ATGCAT 1 cut(s) 1427
MroXI GAANNNNTTC 2 cut(s) 83, 2679
MscI TGGCCA 1 cut(s) 315
MslI CAYNNNNRTG 2 cut(s) 297, 2543
Msp20I TGGCCA 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 523
MspCI CTTAAG 3 cut(s) 215, 1238, 1774
MspR9I CCNGG 4 cut(s) 317, 1498, 1832, 2288
MunI CAATTG 3 cut(s) 1047, 1080, 2467
Mva1269I GAATGC 8 cut(s) 742, 1003, 1064, 1141, 1156, 1427, 1530, 2827
MvaI CCWGG 4 cut(s) 317, 1498, 1832, 2288
MvnI CGCG 1 cut(s) 1918
NdeII GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2535, 2898
NheI GCTAGC 1 cut(s) 1052
NlaIII CATG 8 cut(s) 58, 343, 433, 1408, 1882, 1990, 2210, 2304
NlaIV GGNNCC 6 cut(s) 353, 665, 803, 1687, 1816, 2434
NmuCI GTSAC 2 cut(s) 2296, 2390
NsiI ATGCAT 1 cut(s) 1427
NspI RCATGY 4 cut(s) 343, 1408, 1990, 2304
PaeI GCATGC 1 cut(s) 1408
PaeR7I CTCGAG 1 cut(s) 705
PceI AGGCCT 1 cut(s) 1979
PciI ACATGT 2 cut(s) 1986, 2300
PctI GAATGC 8 cut(s) 742, 1003, 1064, 1141, 1156, 1427, 1530, 2827
PdmI GAANNNNTTC 2 cut(s) 83, 2679
PfeI GAWTC 6 cut(s) 410, 1234, 1695, 1702, 2183, 2916
PkrI GCNGC 8 cut(s) 273, 373, 522, 1511, 1712, 2180, 2746, 2827
PleI GAGTC 7 cut(s) 326, 1024, 1363, 1391, 2111, 2563, 2927
PpsI GAGTC 7 cut(s) 326, 1024, 1363, 1391, 2111, 2563, 2927
PscI ACATGT 2 cut(s) 1986, 2300
PshBI ATTAAT 3 cut(s) 297, 401, 1328
PsiI TTATAA 3 cut(s) 1215, 1824, 2004
Psp1406I AACGTT 1 cut(s) 1346
Psp6I CCWGG 4 cut(s) 315, 1496, 1830, 2286
PspFI CCCAGC 2 cut(s) 190, 1519
PspGI CCWGG 4 cut(s) 315, 1496, 1830, 2286
PspN4I GGNNCC 6 cut(s) 353, 665, 803, 1687, 1816, 2434
PspPI GGNCC 2 cut(s) 2168, 2433
PstI CTGCAG 1 cut(s) 373
PsuI RGATCY 3 cut(s) 663, 1903, 2290
PvuII CAGCTG 1 cut(s) 523
RsaI GTAC 3 cut(s) 353, 1268, 2273
RsaNI GTAC 3 cut(s) 352, 1267, 2272
RseI CAYNNNNRTG 2 cut(s) 297, 2543
SatI GCNGC 8 cut(s) 272, 372, 521, 1510, 1711, 2179, 2745, 2826
Sau3AI GATC 9 cut(s) 549, 663, 888, 905, 1840, 1903, 2290, 2535, 2898
Sau96I GGNCC 2 cut(s) 2168, 2433
SchI GAGTC 7 cut(s) 326, 1025, 1364, 1391, 2111, 2563, 2927
ScrFI CCNGG 4 cut(s) 317, 1498, 1832, 2288
SduI GDGCHC 5 cut(s) 1613, 1690, 1819, 2038, 2818
SexAI ACCWGGT 1 cut(s) 1496
SfaNI GCATC 8 cut(s) 427, 1172, 1201, 1348, 1431, 1962, 2507, 2543
SfcI CTRYAG 1 cut(s) 369
Sfr274I CTCGAG 1 cut(s) 705
SinI GGWCC 2 cut(s) 2168, 2433
SlaI CTCGAG 1 cut(s) 705
SmiMI CAYNNNNRTG 2 cut(s) 297, 2543
SmlI CTYRAG 8 cut(s) 215, 705, 1238, 1774, 1973, 2258, 2854, 2906
SmoI CTYRAG 8 cut(s) 215, 705, 1238, 1774, 1973, 2258, 2854, 2906
SpeI ACTAGT 1 cut(s) 2402
SphI GCATGC 1 cut(s) 1408
SseBI AGGCCT 1 cut(s) 1979
SsiI CCGC 3 cut(s) 1062, 1319, 2745
SspI AATATT 1 cut(s) 1388
SspMI CTAG 9 cut(s) 176, 1053, 1409, 1751, 1793, 2090, 2403, 2511, 2795
StuI AGGCCT 1 cut(s) 1979
StyD4I CCNGG 4 cut(s) 315, 1496, 1830, 2286
StyI CCWWGG 3 cut(s) 1980, 2171, 2521
TaaI ACNGT 8 cut(s) 263, 850, 1435, 1624, 1784, 1970, 2098, 2276
TaiI ACGT 3 cut(s) 1349, 2023, 2028
TaqI TCGA 6 cut(s) 62, 234, 706, 904, 1675, 2593
TatI WGTACW 1 cut(s) 2271
TauI GCSGC 1 cut(s) 2747
TfiI GAWTC 6 cut(s) 410, 1234, 1695, 1702, 2183, 2916
TscAI CASTG 5 cut(s) 853, 1080, 2002, 2281, 2946
TseFI GTSAC 2 cut(s) 2296, 2390
TseI GCWGC 7 cut(s) 271, 371, 520, 1509, 1710, 2178, 2825
Tsp45I GTSAC 2 cut(s) 2296, 2390
TspDTI ATGAA 8 cut(s) 43, 281, 749, 1251, 1551, 1583, 2061, 2657
TspGWI ACGGA 1 cut(s) 1009
TspRI CASTG 5 cut(s) 853, 1080, 2002, 2281, 2946
Vha464I CTTAAG 3 cut(s) 215, 1238, 1774
VpaK11BI GGWCC 2 cut(s) 2168, 2433
VspI ATTAAT 3 cut(s) 297, 401, 1328
XapI RAATTY 7 cut(s) 50, 83, 530, 982, 1276, 1656, 2049
XceI RCATGY 4 cut(s) 343, 1408, 1990, 2304
XcmI CCANNNNNNNNNTGG 1 cut(s) 2344
XhoI CTCGAG 1 cut(s) 705
XmnI GAANNNNTTC 2 cut(s) 83, 2679
XspI CTAG 9 cut(s) 176, 1053, 1409, 1751, 1793, 2090, 2403, 2511, 2795
Zsp2I ATGCAT 1 cut(s) 1427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.