AT4G27320

universal stress protein (USP) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0006464
Physical Location & Seq
Reverse (-)
13678417 .. 13680946
2530 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27320.2

Sequence Viewer

Length: 783 bp
ATGAATCCAGATTCCGATTATCCTCATCTCCCTAACATCAAGATCCACCATCCTTCATCTCCTCGTCACTCTCACCACCACTCTTCCTCCACTCCTTCCGCCGCTACTCCTACCCCAACCGCCGGTGCTCGCCGTAAGATCGGAGTCGCCGTTGACCTTTCCGAAGAAAGCGCTTTCGCTGTTCGCTGGGCTGTCGATCATTACATCCGTCCCGGAGACGCCGTCGTCATTCTCCACGTTTCTCCAACCTCCGTTCTCTTCGGCGCCGATTGGGGACCTCTCCCTCTCCAAACTCCTCCTCCTCCTTCCGCCGCTACCGATCCCGGAGCTCAGCCGAAGCCTAGTCAGGAAGATTTCGATGCGTTTACTTCTTCCAAAGTAGCGGATCTAGCGAAGCCGTTGAAGGAAGCTGGGTTTCCTCATAAGATCCATATAGTGAAAGATCACGATATGAGAGAGAGGCTTTGCTTAGAGACTGAAAGGCTTAATCTAAGCGCCGTGATAATGGGAAGCAGAGGATTTGGTGCTGAGAAGAGAGGAAGTGATGGCAAGCTTGGCTCTGTTAGTGATTATTGTGTTCACCATTGTGTTTGTCCTGTTGTTGTTGTTAGATATCCTGATGATCGTGATGGTCCTGCTCCTCCTGGGAATGTTGGAGCTACCAGGGAAGCTATTGTCACTGTTAAATCACGTAGGGATGATGACGATGATGATGATGAAGATCATGAGGCTAAGATTGCTGCTGCTGCTTCCGATCATCATGAACACATCAAAGATGAGTAA

Protein Analysis

260

Amino Acids

28.11

Weight (kDa)

5.79

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 44 - 204 4.3e-25 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AccB1I GGYRCC 1 cut(s) 263
AciI CCGC 6 cut(s) 99, 102, 120, 309, 312, 383
AclWI GGATC 4 cut(s) 37, 314, 393, 421
AcyI GRCGYC 2 cut(s) 219, 264
AfeI AGCGCT 1 cut(s) 172
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 1 cut(s) 403
AjnI CCWGG 2 cut(s) 643, 662
AleI CACNNNNGTG 1 cut(s) 585
AluBI AGCT 5 cut(s) 329, 410, 553, 659, 671
AluI AGCT 5 cut(s) 329, 410, 553, 659, 671
Alw21I GWGCWC 2 cut(s) 130, 331
Alw26I GTCTC 2 cut(s) 210, 467
AlwI GGATC 4 cut(s) 37, 314, 393, 421
Aor51HI AGCGCT 1 cut(s) 172
ApeKI GCWGC 3 cut(s) 740, 743, 746
AspLEI GCGC 3 cut(s) 173, 266, 497
AspS9I GGNCC 2 cut(s) 275, 632
AsuC2I CCSGG 2 cut(s) 213, 324
AsuHPI GGTGA 2 cut(s) 65, 572
AvaII GGWCC 2 cut(s) 275, 632
BanI GGYRCC 1 cut(s) 263
BanII GRGCYC 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 130, 331
BbvI GCAGC 3 cut(s) 727, 730, 733
BccI CCATC 3 cut(s) 57, 539, 623
BceAI ACGGC 5 cut(s) 117, 134, 206, 382, 482
BciT130I CCWGG 2 cut(s) 645, 664
BcnI CCSGG 2 cut(s) 213, 324
BcoDI GTCTC 2 cut(s) 210, 467
BfaI CTAG 2 cut(s) 342, 389
BfoI RGCGCY 3 cut(s) 174, 267, 498
BisI GCNGC 5 cut(s) 102, 312, 741, 744, 747
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 5 cut(s) 103, 313, 742, 745, 748
Bme1390I CCNGG 4 cut(s) 213, 324, 645, 664
Bme18I GGWCC 2 cut(s) 275, 632
BmgT120I GGNCC 2 cut(s) 275, 632
BmiI GGNNCC 2 cut(s) 265, 276
BmrFI CCNGG 4 cut(s) 213, 324, 645, 664
BmsI GCATC 1 cut(s) 349
Bpu1102I GCTNAGC 1 cut(s) 330
BpuMI CCSGG 2 cut(s) 213, 324
BsaAI YACGTR 1 cut(s) 692
BsaBI GATNNNNATC 1 cut(s) 720
BsaHI GRCGYC 2 cut(s) 219, 264
BsaJI CCNNGG 2 cut(s) 644, 663
BsaXI ACNNNNNCTCC 8 cut(s) 71, 101, 135, 165, 207, 237, 283, 313
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse118I RCCGGY 1 cut(s) 122
Bse8I GATNNNNATC 1 cut(s) 720
BseBI CCWGG 2 cut(s) 645, 664
BseDI CCNNGG 2 cut(s) 644, 663
BseGI GGATG 3 cut(s) 49, 204, 703
BseJI GATNNNNATC 1 cut(s) 720
BseLI CCNNNNNNNGG 1 cut(s) 122
BseMII CTCAG 2 cut(s) 344, 519
BseRI GAGGAG 5 cut(s) 51, 285, 288, 291, 630
BseXI GCAGC 3 cut(s) 727, 730, 733
BseYI CCCAGC 2 cut(s) 186, 410
BshNI GGYRCC 1 cut(s) 263
BsiHKAI GWGCWC 2 cut(s) 130, 331
BsiSI CCGG 3 cut(s) 123, 213, 324
BslFI GGGAC 2 cut(s) 195, 288
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 2 cut(s) 210, 467
BsmBI CGTCTC 1 cut(s) 210
BsmFI GGGAC 2 cut(s) 195, 288
Bsp1286I GDGCHC 2 cut(s) 130, 331
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 6 cut(s) 99, 102, 120, 309, 312, 383
BspCNI CTCAG 2 cut(s) 343, 520
BspHI TCATGA 2 cut(s) 724, 760
BspLI GGNNCC 2 cut(s) 265, 276
BspPI GGATC 4 cut(s) 37, 314, 393, 421
BspT107I GGYRCC 1 cut(s) 263
BsrFI RCCGGY 1 cut(s) 122
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 2 cut(s) 644, 663
BssNI GRCGYC 2 cut(s) 219, 264
Bst2UI CCWGG 2 cut(s) 645, 664
Bst4CI ACNGT 1 cut(s) 682
Bst6I CTCTTC 3 cut(s) 88, 263, 527
BstACI GRCGYC 2 cut(s) 219, 264
BstBAI YACGTR 1 cut(s) 692
BstC8I GCNNGC 2 cut(s) 130, 551
BstDEI CTNAG 5 cut(s) 330, 469, 491, 528, 732
BstF5I GGATG 3 cut(s) 49, 204, 703
BstH2I RGCGCY 3 cut(s) 174, 267, 498
BstHHI GCGC 3 cut(s) 173, 266, 497
BstMAI GTCTC 2 cut(s) 210, 467
BstMWI GCNNNNNNNGC 4 cut(s) 389, 555, 737, 746
BstNI CCWGG 2 cut(s) 645, 664
BstSCI CCNGG 4 cut(s) 211, 322, 643, 662
BstV1I GCAGC 3 cut(s) 727, 730, 733
BstX2I RGATCY 3 cut(s) 42, 385, 426
BstYI RGATCY 3 cut(s) 42, 385, 426
BtsCI GGATG 3 cut(s) 49, 204, 703
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 2 cut(s) 130, 551
CciI TCATGA 2 cut(s) 724, 760
CfoI GCGC 3 cut(s) 173, 266, 497
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 2 cut(s) 275, 632
CseI GACGC 1 cut(s) 227
CviAII CATG 2 cut(s) 725, 761
DdeI CTNAG 5 cut(s) 330, 469, 491, 528, 732
DinI GGCGCC 1 cut(s) 265
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
Eam1104I CTCTTC 3 cut(s) 88, 263, 527
EarI CTCTTC 3 cut(s) 88, 263, 527
EciI GGCGGA 2 cut(s) 88, 298
Ecl136II GAGCTC 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 331
Eco32I GATATC 1 cut(s) 614
Eco47I GGWCC 2 cut(s) 275, 632
Eco47III AGCGCT 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 329
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 643, 662
EcoRV GATATC 1 cut(s) 614
EcoT38I GRGCYC 1 cut(s) 331
EgeI GGCGCC 1 cut(s) 265
EheI GGCGCC 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 2 cut(s) 728, 764
FaiI YATR 6 cut(s) 423, 432, 434, 452, 726, 762
FaqI GGGAC 2 cut(s) 195, 288
FatI CATG 2 cut(s) 724, 760
Fnu4HI GCNGC 5 cut(s) 102, 312, 741, 744, 747
FokI GGATG 3 cut(s) 36, 191, 710
FriOI GRGCYC 1 cut(s) 331
Fsp4HI GCNGC 5 cut(s) 102, 312, 741, 744, 747
FspBI CTAG 2 cut(s) 342, 389
GlaI GCGC 3 cut(s) 172, 265, 496
GluI GCNGC 5 cut(s) 102, 312, 741, 744, 747
GsaI CCCAGC 2 cut(s) 190, 414
HaeII RGCGCY 3 cut(s) 174, 267, 498
HapII CCGG 3 cut(s) 123, 213, 324
HgaI GACGC 1 cut(s) 227
HhaI GCGC 3 cut(s) 173, 266, 497
Hin1I GRCGYC 2 cut(s) 219, 264
Hin1II CATG 2 cut(s) 728, 764
Hin6I GCGC 3 cut(s) 171, 264, 495
HinP1I GCGC 3 cut(s) 171, 264, 495
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 3 cut(s) 4, 11, 144
HpaII CCGG 3 cut(s) 123, 213, 324
HphI GGTGA 2 cut(s) 65, 572
Hpy166II GTNNAC 3 cut(s) 154, 366, 580
Hpy188I TCNGA 4 cut(s) 16, 143, 163, 754
Hpy188III TCNNGA 8 cut(s) 8, 40, 347, 446, 617, 626, 725, 761
Hpy8I GTNNAC 3 cut(s) 154, 366, 580
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 4 cut(s) 63, 105, 315, 397
HpyCH4III ACNGT 1 cut(s) 682
HpyCH4IV ACGT 2 cut(s) 237, 691
HpyF10VI GCNNNNNNNGC 4 cut(s) 389, 555, 737, 746
HpyF3I CTNAG 5 cut(s) 330, 469, 491, 528, 732
HpySE526I ACGT 2 cut(s) 237, 691
Hsp92I GRCGYC 2 cut(s) 219, 264
Hsp92II CATG 2 cut(s) 728, 764
HspAI GCGC 3 cut(s) 171, 264, 495
KasI GGCGCC 1 cut(s) 263
LmnI GCTCC 3 cut(s) 326, 643, 656
Lsp1109I GCAGC 3 cut(s) 727, 730, 733
LweI GCATC 1 cut(s) 349
MaeI CTAG 2 cut(s) 342, 389
MaeII ACGT 2 cut(s) 237, 691
MaeIII GTNAC 2 cut(s) 65, 676
MboII GAAGA 7 cut(s) 75, 176, 250, 362, 363, 544, 731
MflI RGATCY 3 cut(s) 42, 385, 426
MhlI GDGCHC 2 cut(s) 130, 331
Mly113I GGCGCC 1 cut(s) 264
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 2 cut(s) 269, 634
MseI TTAA 2 cut(s) 486, 684
MslI CAYNNNNRTG 1 cut(s) 585
MspI CCGG 3 cut(s) 123, 213, 324
MspR9I CCNGG 4 cut(s) 213, 324, 645, 664
MvaI CCWGG 2 cut(s) 645, 664
MwoI GCNNNNNNNGC 4 cut(s) 389, 555, 737, 746
NarI GGCGCC 1 cut(s) 264
NciI CCSGG 2 cut(s) 213, 324
NlaIII CATG 2 cut(s) 728, 764
NlaIV GGNNCC 2 cut(s) 265, 276
NmuCI GTSAC 2 cut(s) 65, 676
OliI CACNNNNGTG 1 cut(s) 585
PagI TCATGA 2 cut(s) 724, 760
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 2 cut(s) 4, 11
PflFI GACNNNGTC 1 cut(s) 221
PfoI TCCNGGA 2 cut(s) 211, 322
PkrI GCNGC 5 cut(s) 103, 313, 742, 745, 748
PleI GAGTC 1 cut(s) 152
PluTI GGCGCC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 152
Ppu21I YACGTR 1 cut(s) 692
PpuMI RGGWCCY 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 331
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 643, 662
PspFI CCCAGC 2 cut(s) 186, 410
PspGI CCWGG 2 cut(s) 643, 662
PspN4I GGNNCC 2 cut(s) 265, 276
PspPI GGNCC 2 cut(s) 275, 632
PspPPI RGGWCCY 1 cut(s) 275
PsuI RGATCY 3 cut(s) 42, 385, 426
PsyI GACNNNGTC 1 cut(s) 221
RseI CAYNNNNRTG 1 cut(s) 585
SacI GAGCTC 1 cut(s) 331
SaqAI TTAA 2 cut(s) 486, 684
SatI GCNGC 5 cut(s) 102, 312, 741, 744, 747
Sau96I GGNCC 2 cut(s) 275, 632
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 4 cut(s) 213, 324, 645, 664
SduI GDGCHC 2 cut(s) 130, 331
SfaNI GCATC 1 cut(s) 349
SfoI GGCGCC 1 cut(s) 265
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 2 cut(s) 275, 632
SmiMI CAYNNNNRTG 1 cut(s) 585
SsiI CCGC 6 cut(s) 99, 102, 120, 309, 312, 383
SspDI GGCGCC 1 cut(s) 263
SspMI CTAG 2 cut(s) 342, 389
SstI GAGCTC 1 cut(s) 331
StyD4I CCNGG 4 cut(s) 211, 322, 643, 662
TaaI ACNGT 1 cut(s) 682
TaiI ACGT 2 cut(s) 240, 694
TaqI TCGA 2 cut(s) 195, 357
TauI GCSGC 2 cut(s) 104, 314
TfiI GAWTC 2 cut(s) 4, 11
Tru1I TTAA 2 cut(s) 486, 684
Tru9I TTAA 2 cut(s) 486, 684
TscAI CASTG 1 cut(s) 685
TseFI GTSAC 2 cut(s) 65, 676
TseI GCWGC 3 cut(s) 740, 743, 746
Tsp45I GTSAC 2 cut(s) 65, 676
TspDTI ATGAA 4 cut(s) 17, 45, 732, 777
TspGWI ACGGA 2 cut(s) 197, 241
TspRI CASTG 1 cut(s) 685
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 2 cut(s) 275, 632
XspI CTAG 2 cut(s) 342, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.