MD03G1216700.v1.1

Universal stress protein family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006464
Physical Location & Seq
Reverse (-)
30093818 .. 30097602
3785 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1216700.v1.1.491

Sequence Viewer

Length: 798 bp
ATGAATCACCCTCAGCAGCCTCCTCAGCCCCAAGACCCGTCCGATCCCCAACTCCCCCACATCAGAATCCACCACCCCAACTCTCCCCGCCACCACTCCTCCTCCGCCGGCTCCGCCGCCACTCCCACCCCTACCGCCGGTGCCCGCCGCAAGCTCGGCGTCGCCGTCGACCTCTCTGAAGAGAGCGCCCACGCCGTCCGCTGGGCCGTCGATCACTACATCCGCCCCGGTGACGCCGTCATCCTCCTCCACGTCAGCCCCACCTCCGTCCTTTTCGGCGCCGACTGGGGCTCAGTCGACCTCTCCATCAACACCAACGACGACGTCGATTTCGTCGACAACCACGCCCTGCACGATTCCGTCAAGCAGAAGAAACTCGAGAACGACTTCGACGCCCTCACCGCCTCCAAGGTCGCCGATCTCGCCAAGCCCTTGAAGGAGGCGCAGATTCCGTACAAGATCCACATCGTCAAGGACCATGACATGAAGGAGCGGCTCTGCTTGGAGGTTGAGAGGCTAGGGCTCAGTGCCGTGATCATGGGGAGCCGAGGGGTCGGCGCCACCAAGCGTGGGACTGACGGTAGGCTTGGGAGCGTCAGCGATTATTGTGTTCATCACTGCGTTTGTCCCGTTGTTGTTGTCCGGTTTCCGGAGGATGACAGTAGCGGCGTTGCTGGTGGTGGTTCTGGTGGTCCTCCTGTTGCCGCGGCGGTTGTTGCCGTTAAAGAGAATGAGGAGGAAGAGGCCGTGATCAAGCCCGTGACGGTGGTAAAGGAGGAGCATAAGAAAGAAGCTTAG

Protein Analysis

266

Amino Acids

28.41

Weight (kDa)

5.94

Isoelectric Point (pI)

43.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 50 - 215 4.1e-23 Universal stress protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 327
AccB1I GGYRCC 3 cut(s) 140, 278, 557
AccBSI CCGCTC 1 cut(s) 493
AccI GTMKAC 3 cut(s) 168, 297, 336
AccII CGCG 1 cut(s) 707
AccIII TCCGGA 1 cut(s) 649
AclWI GGATC 2 cut(s) 38, 454
AcuI CTGAAG 1 cut(s) 198
AcyI GRCGYC 6 cut(s) 159, 234, 279, 324, 393, 558
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 4 cut(s) 137, 201, 570, 649
AgsI TTSAA 1 cut(s) 436
AjiI CACGTC 1 cut(s) 253
AluBI AGCT 2 cut(s) 154, 794
AluI AGCT 2 cut(s) 154, 794
AlwI GGATC 2 cut(s) 38, 454
Ama87I CYCGRG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 649
AoxI GGCC 2 cut(s) 204, 744
ApeKI GCWGC 1 cut(s) 16
ArsI GACNNNNNNTTYG 2 cut(s) 314, 346
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 4 cut(s) 188, 281, 445, 560
AspS9I GGNCC 3 cut(s) 204, 475, 692
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 2 cut(s) 242, 391
AvaI CYCGRG 1 cut(s) 377
AvaII GGWCC 2 cut(s) 475, 692
BaeGI GKGCMC 1 cut(s) 145
BanI GGYRCC 3 cut(s) 140, 278, 557
BanII GRGCYC 2 cut(s) 293, 525
BbvCI CCTCAGC 2 cut(s) 12, 24
BbvI GCAGC 1 cut(s) 28
BccI CCATC 1 cut(s) 314
BceAI ACGGC 7 cut(s) 149, 179, 191, 221, 515, 704, 731
BclI TGATCA 2 cut(s) 534, 750
BcnI CCSGG 1 cut(s) 228
BfaI CTAG 1 cut(s) 518
BfoI RGCGCY 3 cut(s) 189, 282, 561
BisI GCNGC 7 cut(s) 17, 117, 148, 494, 667, 705, 708
BlsI GCNGC 7 cut(s) 18, 118, 149, 495, 668, 706, 709
Bme1390I CCNGG 1 cut(s) 228
Bme18I GGWCC 2 cut(s) 475, 692
BmeT110I CYCGRG 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 3 cut(s) 204, 475, 692
BmiI GGNNCC 5 cut(s) 112, 142, 280, 545, 559
BmrFI CCNGG 1 cut(s) 228
BmrI ACTGGG 1 cut(s) 295
BmuI ACTGGG 1 cut(s) 295
Bpu10I CCTNAGC 2 cut(s) 12, 24
BpuMI CCSGG 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 464
BsaHI GRCGYC 6 cut(s) 159, 234, 279, 324, 393, 558
BsaJI CCNNGG 4 cut(s) 226, 408, 547, 705
BsaWI WCCGGW 2 cut(s) 642, 649
BsaXI ACNNNNNCTCC 4 cut(s) 83, 86, 113, 116
Bsc4I CCNNNNNNNGG 4 cut(s) 137, 201, 570, 649
Bse118I RCCGGY 2 cut(s) 107, 137
Bse1I ACTGG 1 cut(s) 290
Bse8I GATNNNNATC 1 cut(s) 464
BseAI TCCGGA 1 cut(s) 649
BseDI CCNNGG 4 cut(s) 226, 408, 547, 705
BseGI GGATG 3 cut(s) 219, 240, 661
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 4 cut(s) 137, 201, 570, 649
BseMII CTCAG 4 cut(s) 26, 38, 306, 538
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 6 cut(s) 12, 88, 91, 236, 749, 791
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 1 cut(s) 28
BseYI CCCAGC 1 cut(s) 201
BsgI GTGCAG 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 707
BshFI GGCC 2 cut(s) 206, 746
BshNI GGYRCC 3 cut(s) 140, 278, 557
BsiHKCI CYCGRG 1 cut(s) 377
BsiSI CCGG 5 cut(s) 108, 138, 228, 643, 650
BslFI GGGAC 2 cut(s) 586, 612
BslI CCNNNNNNNGG 4 cut(s) 137, 201, 570, 649
BsmFI GGGAC 2 cut(s) 586, 612
BsnI GGCC 2 cut(s) 206, 746
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 145, 293, 525
Bsp13I TCCGGA 1 cut(s) 649
Bsp143I GATC 6 cut(s) 43, 211, 418, 459, 534, 750
BspANI GGCC 2 cut(s) 206, 746
BspCNI CTCAG 4 cut(s) 25, 37, 305, 537
BspEI TCCGGA 1 cut(s) 649
BspFNI CGCG 1 cut(s) 707
BspLI GGNNCC 5 cut(s) 112, 142, 280, 545, 559
BspPI GGATC 2 cut(s) 38, 454
BspT107I GGYRCC 3 cut(s) 140, 278, 557
BsrBI CCGCTC 1 cut(s) 493
BsrFI RCCGGY 2 cut(s) 107, 137
BsrI ACTGG 1 cut(s) 290
BssAI RCCGGY 2 cut(s) 107, 137
BssECI CCNNGG 4 cut(s) 226, 408, 547, 705
BssMI GATC 6 cut(s) 43, 211, 418, 459, 534, 750
BssNI GRCGYC 6 cut(s) 159, 234, 279, 324, 393, 558
BssT1I CCWWGG 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 581, 662, 766
Bst6I CTCTTC 2 cut(s) 174, 735
BstACI GRCGYC 6 cut(s) 159, 234, 279, 324, 393, 558
BstC8I GCNNGC 3 cut(s) 109, 145, 152
BstDEI CTNAG 5 cut(s) 12, 24, 292, 524, 795
BstDSI CCRYGG 1 cut(s) 705
BstF5I GGATG 3 cut(s) 219, 240, 661
BstFNI CGCG 1 cut(s) 707
BstH2I RGCGCY 3 cut(s) 189, 282, 561
BstHHI GCGC 4 cut(s) 188, 281, 445, 560
BstKTI GATC 6 cut(s) 46, 214, 421, 462, 537, 753
BstMBI GATC 6 cut(s) 43, 211, 418, 459, 534, 750
BstMWI GCNNNNNNNGC 6 cut(s) 25, 113, 156, 401, 422, 716
BstSCI CCNGG 1 cut(s) 226
BstSLI GKGCMC 1 cut(s) 145
BstUI CGCG 1 cut(s) 707
BstV1I GCAGC 1 cut(s) 28
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BsuRI GGCC 2 cut(s) 206, 746
BtgI CCRYGG 1 cut(s) 705
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 3 cut(s) 219, 240, 661
BtsI GCAGTG 1 cut(s) 616
BtsIMutI CAGTG 2 cut(s) 532, 616
Cac8I GCNNGC 3 cut(s) 109, 145, 152
CfoI GCGC 4 cut(s) 188, 281, 445, 560
Cfr10I RCCGGY 2 cut(s) 107, 137
Cfr13I GGNCC 3 cut(s) 204, 475, 692
Cfr42I CCGCGG 1 cut(s) 708
CseI GACGC 4 cut(s) 148, 242, 401, 583
Csp6I GTAC 1 cut(s) 454
CviAII CATG 3 cut(s) 479, 484, 538
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 5 cut(s) 12, 24, 292, 524, 795
DinI GGCGCC 2 cut(s) 280, 559
DpnI GATC 6 cut(s) 45, 213, 420, 461, 536, 752
DpnII GATC 6 cut(s) 43, 211, 418, 459, 534, 750
Eam1104I CTCTTC 2 cut(s) 174, 735
EarI CTCTTC 2 cut(s) 174, 735
EciI GGCGGA 3 cut(s) 94, 103, 212
Eco130I CCWWGG 1 cut(s) 408
Eco24I GRGCYC 2 cut(s) 293, 525
Eco47I GGWCC 2 cut(s) 475, 692
Eco57I CTGAAG 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 408
EcoT38I GRGCYC 2 cut(s) 293, 525
EgeI GGCGCC 2 cut(s) 280, 559
EheI GGCGCC 2 cut(s) 280, 559
ErhI CCWWGG 1 cut(s) 408
FaeI CATG 3 cut(s) 482, 487, 541
FaiI YATR 4 cut(s) 480, 485, 539, 783
FaqI GGGAC 2 cut(s) 586, 612
FatI CATG 3 cut(s) 478, 483, 537
FauI CCCGC 2 cut(s) 95, 152
FbaI TGATCA 2 cut(s) 534, 750
FblI GTMKAC 3 cut(s) 168, 297, 336
Fnu4HI GCNGC 7 cut(s) 17, 117, 148, 494, 667, 705, 708
FokI GGATG 3 cut(s) 206, 227, 668
FriOI GRGCYC 2 cut(s) 293, 525
Fsp4HI GCNGC 7 cut(s) 17, 117, 148, 494, 667, 705, 708
FspBI CTAG 1 cut(s) 518
GlaI GCGC 4 cut(s) 187, 280, 444, 559
GluI GCNGC 7 cut(s) 17, 117, 148, 494, 667, 705, 708
GsaI CCCAGC 1 cut(s) 205
HaeII RGCGCY 3 cut(s) 189, 282, 561
HaeIII GGCC 2 cut(s) 206, 746
HapII CCGG 5 cut(s) 108, 138, 228, 643, 650
HgaI GACGC 4 cut(s) 148, 242, 401, 583
HhaI GCGC 4 cut(s) 188, 281, 445, 560
Hin1I GRCGYC 6 cut(s) 159, 234, 279, 324, 393, 558
Hin1II CATG 3 cut(s) 482, 487, 541
Hin6I GCGC 4 cut(s) 186, 279, 443, 558
HinP1I GCGC 4 cut(s) 186, 279, 443, 558
HincII GTYRAC 3 cut(s) 169, 298, 337
HindII GTYRAC 3 cut(s) 169, 298, 337
HindIII AAGCTT 1 cut(s) 792
HinfI GANTC 4 cut(s) 4, 66, 356, 448
HpaII CCGG 5 cut(s) 108, 138, 228, 643, 650
HphI GGTGA 2 cut(s) 242, 391
Hpy166II GTNNAC 3 cut(s) 169, 298, 337
Hpy188I TCNGA 3 cut(s) 43, 65, 178
Hpy188III TCNNGA 2 cut(s) 379, 650
Hpy8I GTNNAC 3 cut(s) 169, 298, 337
Hpy99I CGWCG 8 cut(s) 164, 170, 212, 323, 326, 329, 338, 395
HpyAV CCTTC 2 cut(s) 430, 481
HpyCH4III ACNGT 3 cut(s) 581, 662, 766
HpyCH4IV ACGT 2 cut(s) 252, 324
HpyCH4V TGCA 1 cut(s) 352
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 113, 156, 401, 422, 716
HpyF3I CTNAG 5 cut(s) 12, 24, 292, 524, 795
HpySE526I ACGT 2 cut(s) 252, 324
Hsp92I GRCGYC 6 cut(s) 159, 234, 279, 324, 393, 558
Hsp92II CATG 3 cut(s) 482, 487, 541
HspAI GCGC 4 cut(s) 186, 279, 443, 558
KasI GGCGCC 2 cut(s) 278, 557
Kpn2I TCCGGA 1 cut(s) 649
KroI GCCGGC 1 cut(s) 107
KroNI GCCGGC 1 cut(s) 109
Ksp22I TGATCA 2 cut(s) 534, 750
KspI CCGCGG 1 cut(s) 708
Kzo9I GATC 6 cut(s) 43, 211, 418, 459, 534, 750
LmnI GCTCC 5 cut(s) 116, 490, 543, 591, 778
Lsp1109I GCAGC 1 cut(s) 28
MaeI CTAG 1 cut(s) 518
MaeII ACGT 2 cut(s) 252, 324
MaeIII GTNAC 2 cut(s) 230, 760
MalI GATC 6 cut(s) 45, 213, 420, 461, 536, 752
MbiI CCGCTC 1 cut(s) 493
MboI GATC 6 cut(s) 43, 211, 418, 459, 534, 750
MboII GAAGA 3 cut(s) 191, 382, 752
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 3 cut(s) 145, 293, 525
Mly113I GGCGCC 2 cut(s) 279, 558
MroI TCCGGA 1 cut(s) 649
MroNI GCCGGC 1 cut(s) 107
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 1 cut(s) 723
MspA1I CMGCKG 2 cut(s) 201, 707
MspI CCGG 5 cut(s) 108, 138, 228, 643, 650
MspR9I CCNGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 707
MwoI GCNNNNNNNGC 6 cut(s) 25, 113, 156, 401, 422, 716
NaeI GCCGGC 1 cut(s) 109
NarI GGCGCC 2 cut(s) 279, 558
NciI CCSGG 1 cut(s) 228
NdeII GATC 6 cut(s) 43, 211, 418, 459, 534, 750
NgoMIV GCCGGC 1 cut(s) 107
NlaIII CATG 3 cut(s) 482, 487, 541
NlaIV GGNNCC 5 cut(s) 112, 142, 280, 545, 559
NmeAIII GCCGAG 2 cut(s) 135, 572
NmuCI GTSAC 2 cut(s) 230, 760
PaeR7I CTCGAG 1 cut(s) 377
PcsI WCGNNNNNNNCGW 5 cut(s) 162, 324, 330, 333, 351
PdiI GCCGGC 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 4 cut(s) 4, 66, 356, 448
PflFI GACNNNGTC 2 cut(s) 236, 323
PkrI GCNGC 7 cut(s) 18, 118, 149, 495, 668, 706, 709
PluTI GGCGCC 2 cut(s) 282, 561
PspFI CCCAGC 1 cut(s) 201
PspN4I GGNNCC 5 cut(s) 112, 142, 280, 545, 559
PspPI GGNCC 3 cut(s) 204, 475, 692
PsuI RGATCY 1 cut(s) 459
PsyI GACNNNGTC 2 cut(s) 236, 323
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SacII CCGCGG 1 cut(s) 708
SalI GTCGAC 3 cut(s) 167, 296, 335
SaqAI TTAA 1 cut(s) 723
SatI GCNGC 7 cut(s) 17, 117, 148, 494, 667, 705, 708
Sau3AI GATC 6 cut(s) 43, 211, 418, 459, 534, 750
Sau96I GGNCC 3 cut(s) 204, 475, 692
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 3 cut(s) 145, 293, 525
SetI ASST 9 cut(s) 156, 174, 255, 266, 303, 327, 414, 510, 796
SfoI GGCGCC 2 cut(s) 280, 559
Sfr274I CTCGAG 1 cut(s) 377
Sfr303I CCGCGG 1 cut(s) 708
SgrAI CRCCGGYG 1 cut(s) 137
SgrBI CCGCGG 1 cut(s) 708
SinI GGWCC 2 cut(s) 475, 692
SlaI CTCGAG 1 cut(s) 377
SmlI CTYRAG 1 cut(s) 377
SmoI CTYRAG 1 cut(s) 377
SspDI GGCGCC 2 cut(s) 278, 557
SspMI CTAG 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 3 cut(s) 581, 662, 766
TaiI ACGT 2 cut(s) 255, 327
TaqI TCGA 7 cut(s) 168, 210, 297, 327, 336, 378, 390
TauI GCSGC 6 cut(s) 119, 150, 496, 669, 707, 710
TfiI GAWTC 4 cut(s) 4, 66, 356, 448
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TscAI CASTG 2 cut(s) 532, 623
TseFI GTSAC 2 cut(s) 230, 760
TseI GCWGC 1 cut(s) 16
Tsp45I GTSAC 2 cut(s) 230, 760
TspDTI ATGAA 3 cut(s) 17, 500, 602
TspGWI ACGGA 3 cut(s) 256, 349, 441
TspRI CASTG 2 cut(s) 532, 623
Tth111I GACNNNGTC 2 cut(s) 236, 323
VpaK11BI GGWCC 2 cut(s) 475, 692
XhoI CTCGAG 1 cut(s) 377
XmiI GTMKAC 3 cut(s) 168, 297, 336
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 1 cut(s) 518
ZraI GACGTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.