AT4G30993
ERF Family

Calcineurin-like metallo-phosphoesterase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0010859
Physical Location & Seq
Forward (+)
15098041 .. 15100783
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30993.1

Sequence Viewer

Length: 630 bp
ATGAAACAATCGTCATGGGGTTGCACAATCTTCATCCAATTATCTCTATGTCTCATCATCTATGTGTCTCTGCATTCGGGTCACCCATTCTTCTTCTCTAGTGACGACGGTGACAATGCCACACCTCTAGATCTTCATTTCATCTCCGTTGCCGGCGGTTTCAGGCCTCTTAACCACCAAACTCGCCTTCTCCGATTGATGGAGAAAGTTGCAGAAACTTACAAGGCCAAATTTGTTGTGAGCTCAAGTGAACAAGGAGAAGAAGATCCATTCCTGCAAAATGCGACTCGACTGTCTTCTTCACTGAAACTTCCATGGTACACTAGAATGAAAGGATCTGGATATTTCAGAGAGCACATCAAAATGCCTTTTGGTGGATCCTTGGATGTTGTTTTCGTAGACACTGGCTCACTCAAGCAGGAAGTGCTTGGTGGAGCAGTGAATGCCTATATGATCAGTCAATTGAAAGGGTTGACAAGGATTCTAAAAGCAGTAGACGGAGACTGGCGTATTGTGGTTGGATCAGACCCGTTACTCGCCTATACTCTAACTAAGGAACCAGAGGAACCTAAGAGAGTGGCAAGGACATTTCATCAAATCATGACCAAATATGGAGTGGTAAGACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

23.49

Weight (kDa)

9.04

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30993 AT4G30993 AT4G30993
fragaria_vesca FvH4_1g01190 FvH4_1g01190
malus_domestica MD02G1010000.v1.1 MD02G1010100.v1.1
prunus_persica Prupe.7G260000_v2.0.a1
pyrus_communis pycom02g00880 pycom15g13910
rosa_chinensis RchiOBHm_Chr2g0085991
rosa_laevigata RLG00000015732
rosa_multiflora Rmu_ssc0000355.1_g000024
rosa_roxburghii Rroxscaffold_2G00154870
rosa_rugosa Rorug01G0464900
rosa_samantha Rh2AG014100 Rh2BG014600 Rh2CG015100 Rh2DG016400
rosa_wichuraiana Rw2G001250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 292
AccI GTMKAC 2 cut(s) 399, 495
AciI CCGC 1 cut(s) 156
AclWI GGATC 5 cut(s) 260, 343, 372, 385, 529
AcsI RAATTY 1 cut(s) 230
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 199, 374
AgsI TTSAA 1 cut(s) 466
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AluBI AGCT 1 cut(s) 243
AluI AGCT 1 cut(s) 243
Alw21I GWGCWC 2 cut(s) 245, 357
Alw26I GTCTC 3 cut(s) 56, 72, 495
AlwI GGATC 5 cut(s) 260, 343, 372, 385, 529
AoxI GGCC 2 cut(s) 164, 225
ApoI RAATTY 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 74, 122
BamHI GGATCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 245
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 2 cut(s) 245, 357
BccI CCATC 1 cut(s) 193
BclI TGATCA 1 cut(s) 453
BcoDI GTCTC 3 cut(s) 56, 72, 495
BfaI CTAG 3 cut(s) 99, 128, 324
BglII AGATCT 1 cut(s) 130
BmiI GGNNCC 3 cut(s) 379, 558, 567
BpiI GAAGAC 1 cut(s) 288
BpuEI CTTGAG 2 cut(s) 229, 398
BsaJI CCNNGG 2 cut(s) 314, 381
Bsc4I CCNNNNNNNGG 2 cut(s) 199, 374
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 409, 509
BseDI CCNNGG 2 cut(s) 314, 381
BseGI GGATG 2 cut(s) 33, 391
BseLI CCNNNNNNNGG 2 cut(s) 199, 374
BseNI ACTGG 2 cut(s) 409, 509
BshFI GGCC 2 cut(s) 166, 227
BsiHKAI GWGCWC 2 cut(s) 245, 357
BsiSI CCGG 1 cut(s) 153
BslI CCNNNNNNNGG 2 cut(s) 199, 374
BsmAI GTCTC 3 cut(s) 56, 72, 495
BsmI GAATGC 2 cut(s) 73, 448
BsnI GGCC 2 cut(s) 166, 227
Bsp1286I GDGCHC 2 cut(s) 245, 357
Bsp143I GATC 6 cut(s) 130, 265, 335, 377, 453, 521
Bsp19I CCATGG 1 cut(s) 314
BspACI CCGC 1 cut(s) 156
BspANI GGCC 2 cut(s) 166, 227
BspHI TCATGA 1 cut(s) 600
BspLI GGNNCC 3 cut(s) 379, 558, 567
BspPI GGATC 5 cut(s) 260, 343, 372, 385, 529
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 409, 509
BssAI RCCGGY 1 cut(s) 152
BssECI CCNNGG 2 cut(s) 314, 381
BssMI GATC 6 cut(s) 130, 265, 335, 377, 453, 521
BssT1I CCWWGG 2 cut(s) 314, 381
Bst4CI ACNGT 2 cut(s) 110, 294
BstAPI GCANNNNNTGC 2 cut(s) 424, 443
BstC8I GCNNGC 1 cut(s) 154
BstDEI CTNAG 2 cut(s) 552, 570
BstDSI CCRYGG 1 cut(s) 314
BstEII GGTNACC 1 cut(s) 80
BstF5I GGATG 2 cut(s) 33, 391
BstKTI GATC 6 cut(s) 133, 268, 338, 380, 456, 524
BstMAI GTCTC 3 cut(s) 56, 72, 495
BstMBI GATC 6 cut(s) 130, 265, 335, 377, 453, 521
BstMWI GCNNNNNNNGC 2 cut(s) 424, 443
BstPI GGTNACC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 288
BstX2I RGATCY 4 cut(s) 130, 265, 335, 377
BstYI RGATCY 4 cut(s) 130, 265, 335, 377
BsuRI GGCC 2 cut(s) 166, 227
BtgI CCRYGG 1 cut(s) 314
BtsCI GGATG 2 cut(s) 33, 391
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 3 cut(s) 302, 402, 444
Cac8I GCNNGC 1 cut(s) 154
CciI TCATGA 1 cut(s) 600
Cfr10I RCCGGY 1 cut(s) 152
Csp6I GTAC 1 cut(s) 319
CviAII CATG 3 cut(s) 15, 315, 601
CviJI RGCY 4 cut(s) 166, 227, 243, 408
CviKI_1 RGCY 4 cut(s) 166, 227, 243, 408
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 2 cut(s) 552, 570
DpnI GATC 6 cut(s) 132, 267, 337, 379, 455, 523
DpnII GATC 6 cut(s) 130, 265, 335, 377, 453, 521
DrdI GACNNNNNNGTC 1 cut(s) 292
DseDI GACNNNNNNGTC 1 cut(s) 292
Ecl136II GAGCTC 1 cut(s) 243
Eco130I CCWWGG 2 cut(s) 314, 381
Eco147I AGGCCT 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 243
Eco91I GGTNACC 1 cut(s) 80
EcoICRI GAGCTC 1 cut(s) 243
EcoO65I GGTNACC 1 cut(s) 80
EcoT14I CCWWGG 2 cut(s) 314, 381
EcoT38I GRGCYC 1 cut(s) 245
ErhI CCWWGG 2 cut(s) 314, 381
FaeI CATG 3 cut(s) 18, 318, 604
FaiI YATR 9 cut(s) 16, 49, 63, 316, 450, 452, 543, 602, 612
FatI CATG 3 cut(s) 14, 314, 600
FbaI TGATCA 1 cut(s) 453
FblI GTMKAC 2 cut(s) 399, 495
FokI GGATG 2 cut(s) 20, 398
FriOI GRGCYC 1 cut(s) 245
FspBI CTAG 3 cut(s) 99, 128, 324
HaeIII GGCC 2 cut(s) 166, 227
HapII CCGG 1 cut(s) 153
Hin1II CATG 3 cut(s) 18, 318, 604
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HinfI GANTC 2 cut(s) 286, 481
HpaII CCGG 1 cut(s) 153
HphI GGTGA 2 cut(s) 74, 122
Hpy166II GTNNAC 5 cut(s) 251, 321, 400, 474, 496
Hpy188I TCNGA 3 cut(s) 194, 350, 526
Hpy188III TCNNGA 3 cut(s) 128, 339, 601
Hpy8I GTNNAC 5 cut(s) 251, 321, 400, 474, 496
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 1 cut(s) 197
HpyCH4III ACNGT 2 cut(s) 110, 294
HpyCH4V TGCA 4 cut(s) 24, 73, 212, 277
HpyF10VI GCNNNNNNNGC 2 cut(s) 424, 443
HpyF3I CTNAG 2 cut(s) 552, 570
Hsp92II CATG 3 cut(s) 18, 318, 604
KroI GCCGGC 1 cut(s) 152
KroNI GCCGGC 1 cut(s) 154
Ksp22I TGATCA 1 cut(s) 453
Kzo9I GATC 6 cut(s) 130, 265, 335, 377, 453, 521
LmnI GCTCC 1 cut(s) 434
LpnPI CCDG 8 cut(s) 148, 166, 287, 324, 390, 404, 490, 573
MaeI CTAG 3 cut(s) 99, 128, 324
MaeIII GTNAC 4 cut(s) 80, 101, 110, 531
MalI GATC 6 cut(s) 132, 267, 337, 379, 455, 523
MboI GATC 6 cut(s) 130, 265, 335, 377, 453, 521
MboII GAAGA 8 cut(s) 22, 82, 85, 125, 272, 275, 288, 291
MfeI CAATTG 1 cut(s) 461
MflI RGATCY 4 cut(s) 130, 265, 335, 377
MhlI GDGCHC 2 cut(s) 245, 357
MluCI AATT 3 cut(s) 38, 230, 461
MlyI GAGTC 1 cut(s) 280
MmeI TCCRAC 1 cut(s) 499
MnlI CCTC 3 cut(s) 135, 177, 556
MroNI GCCGGC 1 cut(s) 152
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 3 cut(s) 62, 326, 362
MspI CCGG 1 cut(s) 153
MunI CAATTG 1 cut(s) 461
Mva1269I GAATGC 2 cut(s) 73, 448
MwoI GCNNNNNNNGC 2 cut(s) 424, 443
NaeI GCCGGC 1 cut(s) 154
NcoI CCATGG 1 cut(s) 314
NdeII GATC 6 cut(s) 130, 265, 335, 377, 453, 521
NgoMIV GCCGGC 1 cut(s) 152
NlaIII CATG 3 cut(s) 18, 318, 604
NlaIV GGNNCC 3 cut(s) 379, 558, 567
NmuCI GTSAC 3 cut(s) 80, 101, 110
PagI TCATGA 1 cut(s) 600
PceI AGGCCT 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 190
PctI GAATGC 2 cut(s) 73, 448
PdiI GCCGGC 1 cut(s) 154
PfeI GAWTC 1 cut(s) 481
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 80
PspN4I GGNNCC 3 cut(s) 379, 558, 567
PsuI RGATCY 4 cut(s) 130, 265, 335, 377
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 3 cut(s) 62, 326, 362
SacI GAGCTC 1 cut(s) 245
SaqAI TTAA 1 cut(s) 171
Sau3AI GATC 6 cut(s) 130, 265, 335, 377, 453, 521
SchI GAGTC 1 cut(s) 280
SduI GDGCHC 2 cut(s) 245, 357
SetI ASST 3 cut(s) 127, 245, 571
SmiMI CAYNNNNRTG 3 cut(s) 62, 326, 362
SmlI CTYRAG 2 cut(s) 244, 413
SmoI CTYRAG 2 cut(s) 244, 413
Sse9I AATT 3 cut(s) 38, 230, 461
SseBI AGGCCT 1 cut(s) 166
SsiI CCGC 1 cut(s) 156
SspMI CTAG 3 cut(s) 99, 128, 324
SstI GAGCTC 1 cut(s) 245
StuI AGGCCT 1 cut(s) 166
StyI CCWWGG 2 cut(s) 314, 381
TaaI ACNGT 2 cut(s) 110, 294
TaqI TCGA 1 cut(s) 289
TasI AATT 3 cut(s) 38, 230, 461
TfiI GAWTC 1 cut(s) 481
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 3 cut(s) 309, 409, 444
TseFI GTSAC 3 cut(s) 80, 101, 110
Tsp45I GTSAC 3 cut(s) 80, 101, 110
TspDTI ATGAA 6 cut(s) 17, 22, 125, 130, 344, 581
TspGWI ACGGA 2 cut(s) 136, 513
TspRI CASTG 3 cut(s) 309, 409, 444
XapI RAATTY 1 cut(s) 230
XbaI TCTAGA 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmiI GTMKAC 2 cut(s) 399, 495
XspI CTAG 3 cut(s) 99, 128, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.