MD02G1010000.v1.1
ERF Family

Calcineurin-like metallo-phosphoesterase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010859
Physical Location & Seq
Forward (+)
660046 .. 661675
1630 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1010000.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGAGAAAAAACCCACTTGGGTTCTTACACTGGTGACGCAGGTGAGTCTGTGCTTTTGTCTCTACCTTGCTTTTCAGCTGGGTCAGCCTTGGAGCTCTGTCCGCCATGGCAGAAGTGAGAGAAGGCCGCTGGATCTTTACTTCATCAGTGTCGGAGGAGGATTCCGGCCTCCCACCCGACAGACCCATCTTCTCAAGCAGATGGAGAAGGTGGCAAAGAAGTACAAGGCAGAGTTTGTGGTGGACGTTAGCGATCTTGGGGAAGATGATCCGCTCATGCAGAATGTAAGATCTCGTAGGAAACTGTTACACGAATGGATAAAGTTGATAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

13.0

Weight (kDa)

9.59

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30993 AT4G30993 AT4G30993
fragaria_vesca FvH4_1g01190 FvH4_1g01190
malus_domestica MD02G1010000.v1.1 MD02G1010100.v1.1
prunus_persica Prupe.7G260000_v2.0.a1
pyrus_communis pycom02g00880 pycom15g13910
rosa_chinensis RchiOBHm_Chr2g0085991
rosa_laevigata RLG00000015732
rosa_multiflora Rmu_ssc0000355.1_g000024
rosa_roxburghii Rroxscaffold_2G00154870
rosa_rugosa Rorug01G0464900
rosa_samantha Rh2AG014100 Rh2BG014600 Rh2CG015100 Rh2DG016400
rosa_wichuraiana Rw2G001250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccBSI CCGCTC 1 cut(s) 274
AciI CCGC 3 cut(s) 103, 128, 272
AclWI GGATC 2 cut(s) 141, 263
AfaI GTAC 1 cut(s) 224
AluBI AGCT 2 cut(s) 79, 96
AluI AGCT 2 cut(s) 79, 96
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 1 cut(s) 65
AlwI GGATC 2 cut(s) 141, 263
AoxI GGCC 2 cut(s) 125, 167
AsuHPI GGTGA 2 cut(s) 46, 55
BanII GRGCYC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 98
BccI CCATC 2 cut(s) 195, 196
BcoDI GTCTC 1 cut(s) 65
BfuAI ACCTGC 1 cut(s) 31
BglII AGATCT 1 cut(s) 290
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
BpuEI CTTGAG 1 cut(s) 179
BsaJI CCNNGG 2 cut(s) 89, 106
Bse1I ACTGG 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 89, 106
BseNI ACTGG 1 cut(s) 36
BseRI GAGGAG 1 cut(s) 171
BseYI CCCAGC 1 cut(s) 79
BshFI GGCC 2 cut(s) 127, 169
BsiHKAI GWGCWC 1 cut(s) 98
BsiSI CCGG 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 65
BsnI GGCC 2 cut(s) 127, 169
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 4 cut(s) 133, 253, 268, 290
Bsp19I CCATGG 1 cut(s) 106
BspACI CCGC 3 cut(s) 103, 128, 272
BspANI GGCC 2 cut(s) 127, 169
BspMI ACCTGC 1 cut(s) 31
BspPI GGATC 2 cut(s) 141, 263
BsrBI CCGCTC 1 cut(s) 274
BsrI ACTGG 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 89, 106
BssMI GATC 4 cut(s) 133, 253, 268, 290
BssT1I CCWWGG 2 cut(s) 89, 106
Bst4CI ACNGT 1 cut(s) 306
BstDSI CCRYGG 1 cut(s) 106
BstKTI GATC 4 cut(s) 136, 256, 271, 293
BstMAI GTCTC 1 cut(s) 65
BstMBI GATC 4 cut(s) 133, 253, 268, 290
BstMWI GCNNNNNNNGC 2 cut(s) 85, 102
BstX2I RGATCY 2 cut(s) 133, 290
BstYI RGATCY 2 cut(s) 133, 290
BsuRI GGCC 2 cut(s) 127, 169
BtgI CCRYGG 1 cut(s) 106
BtsIMutI CAGTG 2 cut(s) 29, 154
BveI ACCTGC 1 cut(s) 31
CseI GACGC 1 cut(s) 46
Csp6I GTAC 1 cut(s) 223
CviAII CATG 2 cut(s) 107, 277
CviJI RGCY 5 cut(s) 79, 88, 96, 127, 169
CviKI_1 RGCY 5 cut(s) 79, 88, 96, 127, 169
CviQI GTAC 1 cut(s) 223
DpnI GATC 4 cut(s) 135, 255, 270, 292
DpnII GATC 4 cut(s) 133, 253, 268, 290
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 2 cut(s) 89, 106
Eco24I GRGCYC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 96
EcoICRI GAGCTC 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 89, 106
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 2 cut(s) 89, 106
FaeI CATG 2 cut(s) 110, 280
FaiI YATR 2 cut(s) 108, 278
FatI CATG 2 cut(s) 106, 276
Fnu4HI GCNGC 1 cut(s) 128
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 1 cut(s) 128
GluI GCNGC 1 cut(s) 128
GsaI CCCAGC 1 cut(s) 83
HaeIII GGCC 2 cut(s) 127, 169
HapII CCGG 1 cut(s) 166
HgaI GACGC 1 cut(s) 46
Hin1II CATG 2 cut(s) 110, 280
HinfI GANTC 2 cut(s) 46, 162
HpaII CCGG 1 cut(s) 166
HphI GGTGA 2 cut(s) 46, 55
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 155
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 2 cut(s) 117, 202
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 1 cut(s) 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 85, 102
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 2 cut(s) 110, 280
Kzo9I GATC 4 cut(s) 133, 253, 268, 290
LmnI GCTCC 1 cut(s) 93
LpnPI CCDG 5 cut(s) 17, 26, 65, 116, 179
MaeII ACGT 1 cut(s) 246
MaeIII GTNAC 2 cut(s) 34, 306
MalI GATC 4 cut(s) 135, 255, 270, 292
MbiI CCGCTC 1 cut(s) 274
MboI GATC 4 cut(s) 133, 253, 268, 290
MboII GAAGA 2 cut(s) 182, 275
MflI RGATCY 2 cut(s) 133, 290
MhlI GDGCHC 1 cut(s) 98
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 3 cut(s) 149, 152, 180
MspA1I CMGCKG 2 cut(s) 79, 130
MspI CCGG 1 cut(s) 166
MwoI GCNNNNNNNGC 2 cut(s) 85, 102
NcoI CCATGG 1 cut(s) 106
NdeII GATC 4 cut(s) 133, 253, 268, 290
NlaIII CATG 2 cut(s) 110, 280
NmuCI GTSAC 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 31
PfeI GAWTC 1 cut(s) 162
PkrI GCNGC 1 cut(s) 129
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
Psp124BI GAGCTC 1 cut(s) 98
PspFI CCCAGC 1 cut(s) 79
PsuI RGATCY 2 cut(s) 133, 290
PvuII CAGCTG 1 cut(s) 79
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
SacI GAGCTC 1 cut(s) 98
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 4 cut(s) 133, 253, 268, 290
SchI GAGTC 1 cut(s) 55
SduI GDGCHC 1 cut(s) 98
SetI ASST 6 cut(s) 45, 69, 81, 98, 213, 249
SmlI CTYRAG 1 cut(s) 194
SmoI CTYRAG 1 cut(s) 194
SsiI CCGC 3 cut(s) 103, 128, 272
SstI GAGCTC 1 cut(s) 98
StyI CCWWGG 2 cut(s) 89, 106
TaaI ACNGT 1 cut(s) 306
TaiI ACGT 1 cut(s) 249
TatI WGTACW 1 cut(s) 222
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 1 cut(s) 162
TscAI CASTG 2 cut(s) 36, 154
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 1 cut(s) 133
TspRI CASTG 2 cut(s) 36, 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.