MD02G1010100.v1.1
ERF Family

Calcineurin-like metallo-phosphoesterase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010859
Physical Location & Seq
Forward (+)
661677 .. 668433
6757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1010100.v1.1.491

Sequence Viewer

Length: 594 bp
ATGCTGGATATATTCGCTTTACAGGGCACCCTCCATTTCTCGACACTGAAAGTTCCCTGGTACACAACCAGAGTTTCGGAAGGAAAAGGAGGAGGTTACTTCCAGAAGAAGATTAAGTTACCTTACGAGAAAACCTTGGATGTCATTGTGGTGGATACTGGATTATTTCAGCGATGTTGTAGAAACACAACGAAGCTTGAAGAAATTGTGAATGTTCATGAGTTGCGCGTAGTCGTTGGATTCCATCCACTGGCTATTTGTGAAGATGGTGAGGAGAGAATGGAAGAAACGCAAGTTTTCGAGTCTCTACGAAGTACTATGACGAAATTTGAAGTGAACGCATACTTAAGCGGGCAGGGTTGCGCTAACAGCCCCCGATCAAGTAGTCTGAGCTACATCAGGAACCCGGGCAAGATGGCAGCAGGTGCTCATCTTGCTTCCGTACATGAAACATCAGCATCTAGAAGAAAATTGGAAGACGGGTTTTTTCTTCATAGAGTGAGCTCATTAGAGATTGAAACCTACTTCGTTACATCAGCTGGAGAAGTTGTGCATAGATCTGTACTCCATCAGAGAGGAAAGGAGATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

22.17

Weight (kDa)

7.74

Isoelectric Point (pI)

35.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30993 AT4G30993 AT4G30993
fragaria_vesca FvH4_1g01190 FvH4_1g01190
malus_domestica MD02G1010000.v1.1 MD02G1010100.v1.1
prunus_persica Prupe.7G260000_v2.0.a1
pyrus_communis pycom02g00880 pycom15g13910
rosa_chinensis RchiOBHm_Chr2g0085991
rosa_laevigata RLG00000015732
rosa_multiflora Rmu_ssc0000355.1_g000024
rosa_roxburghii Rroxscaffold_2G00154870
rosa_rugosa Rorug01G0464900
rosa_samantha Rh2AG014100 Rh2BG014600 Rh2CG015100 Rh2DG016400
rosa_wichuraiana Rw2G001250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 413
Acc36I ACCTGC 1 cut(s) 413
AccB1I GGYRCC 1 cut(s) 26
AccB7I CCANNNNNTGG 1 cut(s) 250
AccII CGCG 1 cut(s) 228
AciI CCGC 1 cut(s) 351
AcsI RAATTY 1 cut(s) 326
AfaI GTAC 4 cut(s) 62, 316, 444, 564
AfiI CCNNNNNNNGG 1 cut(s) 250
AflII CTTAAG 1 cut(s) 346
AgsI TTSAA 3 cut(s) 200, 332, 518
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 4 cut(s) 196, 393, 504, 539
AluI AGCT 4 cut(s) 196, 393, 504, 539
Alw21I GWGCWC 2 cut(s) 430, 506
Alw26I GTCTC 1 cut(s) 309
Ama87I CYCGRG 1 cut(s) 406
ApeKI GCWGC 1 cut(s) 419
ApoI RAATTY 1 cut(s) 326
AspLEI GCGC 2 cut(s) 228, 365
AsuC2I CCSGG 2 cut(s) 407, 408
AsuHPI GGTGA 1 cut(s) 281
AvaI CYCGRG 1 cut(s) 406
BaeGI GKGCMC 1 cut(s) 29
BanI GGYRCC 1 cut(s) 26
BanII GRGCYC 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 483
Bbv12I GWGCWC 2 cut(s) 430, 506
BbvI GCAGC 1 cut(s) 431
BccI CCATC 4 cut(s) 252, 260, 409, 576
BciT130I CCWGG 1 cut(s) 58
BciVI GTATCC 1 cut(s) 148
BcnI CCSGG 2 cut(s) 407, 408
BcoDI GTCTC 1 cut(s) 309
BfaI CTAG 1 cut(s) 462
BfrI CTTAAG 1 cut(s) 346
BfuAI ACCTGC 1 cut(s) 413
BfuI GTATCC 1 cut(s) 148
BglII AGATCT 1 cut(s) 557
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
BmcAI AGTACT 1 cut(s) 316
Bme1390I CCNGG 3 cut(s) 58, 407, 408
BmeT110I CYCGRG 1 cut(s) 406
BmiI GGNNCC 2 cut(s) 28, 404
BmrFI CCNGG 3 cut(s) 58, 407, 408
BmsI GCATC 1 cut(s) 467
BpiI GAAGAC 1 cut(s) 483
BpmI CTGGAG 1 cut(s) 561
BpuMI CCSGG 2 cut(s) 407, 408
BsaJI CCNNGG 3 cut(s) 56, 135, 406
BsaXI ACNNNNNCTCC 3 cut(s) 534, 564, 575
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 2 cut(s) 163, 255
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 3 cut(s) 56, 135, 406
BseGI GGATG 2 cut(s) 145, 244
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 2 cut(s) 163, 255
BseRI GAGGAG 2 cut(s) 105, 287
BseSI GKGCMC 1 cut(s) 29
BseXI GCAGC 1 cut(s) 431
Bsh1236I CGCG 1 cut(s) 228
BshNI GGYRCC 1 cut(s) 26
BsiHKAI GWGCWC 2 cut(s) 430, 506
BsiHKCI CYCGRG 1 cut(s) 406
BsiSI CCGG 1 cut(s) 407
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 1 cut(s) 309
BsoBI CYCGRG 1 cut(s) 406
Bsp1286I GDGCHC 3 cut(s) 29, 430, 506
Bsp143I GATC 3 cut(s) 377, 557, 585
BspACI CCGC 1 cut(s) 351
BspCNI CTCAG 1 cut(s) 381
BspFNI CGCG 1 cut(s) 228
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 2 cut(s) 28, 404
BspMI ACCTGC 1 cut(s) 413
BspT107I GGYRCC 1 cut(s) 26
BspTI CTTAAG 1 cut(s) 346
BsrI ACTGG 2 cut(s) 163, 255
BssECI CCNNGG 3 cut(s) 56, 135, 406
BssMI GATC 3 cut(s) 377, 557, 585
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 1 cut(s) 58
BstAFI CTTAAG 1 cut(s) 346
BstAPI GCANNNNNTGC 1 cut(s) 425
BstC8I GCNNGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 389
BstF5I GGATG 2 cut(s) 145, 244
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 2 cut(s) 228, 365
BstKTI GATC 3 cut(s) 380, 560, 588
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 3 cut(s) 377, 557, 585
BstMWI GCNNNNNNNGC 3 cut(s) 369, 425, 434
BstNI CCWGG 1 cut(s) 58
BstSCI CCNGG 3 cut(s) 56, 405, 406
BstSLI GKGCMC 1 cut(s) 29
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 431
BstV2I GAAGAC 1 cut(s) 483
BstX2I RGATCY 1 cut(s) 557
BstYI RGATCY 1 cut(s) 557
BsuI GTATCC 1 cut(s) 148
BtgZI GCGATG 1 cut(s) 187
BtsCI GGATG 2 cut(s) 145, 244
BtsIMutI CAGTG 2 cut(s) 44, 248
BveI ACCTGC 1 cut(s) 413
Cac8I GCNNGC 1 cut(s) 353
CciI TCATGA 1 cut(s) 217
CfoI GCGC 2 cut(s) 228, 365
Cfr9I CCCGGG 1 cut(s) 406
Csp6I GTAC 4 cut(s) 61, 315, 443, 563
CviAII CATG 3 cut(s) 218, 446, 589
CviJI RGCY 6 cut(s) 196, 254, 372, 393, 504, 539
CviKI_1 RGCY 6 cut(s) 196, 254, 372, 393, 504, 539
CviQI GTAC 4 cut(s) 61, 315, 443, 563
DdeI CTNAG 1 cut(s) 389
DpnI GATC 3 cut(s) 379, 559, 587
DpnII GATC 3 cut(s) 377, 557, 585
Ecl136II GAGCTC 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 506
Eco53kI GAGCTC 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 406
EcoICRI GAGCTC 1 cut(s) 504
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 506
ErhI CCWWGG 1 cut(s) 135
FaeI CATG 3 cut(s) 221, 449, 592
FaiI YATR 8 cut(s) 11, 219, 320, 343, 447, 495, 555, 590
FatI CATG 3 cut(s) 217, 445, 588
FauI CCCGC 1 cut(s) 344
Fnu4HI GCNGC 1 cut(s) 420
FokI GGATG 2 cut(s) 152, 231
FriOI GRGCYC 1 cut(s) 506
Fsp4HI GCNGC 1 cut(s) 420
FspBI CTAG 1 cut(s) 462
GlaI GCGC 2 cut(s) 227, 364
GluI GCNGC 1 cut(s) 420
GsuI CTGGAG 1 cut(s) 561
HapII CCGG 1 cut(s) 407
HhaI GCGC 2 cut(s) 228, 365
Hin1II CATG 3 cut(s) 221, 449, 592
Hin6I GCGC 2 cut(s) 226, 363
HinP1I GCGC 2 cut(s) 226, 363
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 2 cut(s) 240, 302
HpaII CCGG 1 cut(s) 407
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 2 cut(s) 63, 337
Hpy188I TCNGA 3 cut(s) 79, 390, 573
Hpy188III TCNNGA 5 cut(s) 40, 103, 218, 400, 462
Hpy8I GTNNAC 2 cut(s) 63, 337
HpyAV CCTTC 1 cut(s) 74
HpyCH4V TGCA 1 cut(s) 553
HpyF10VI GCNNNNNNNGC 3 cut(s) 369, 425, 434
HpyF3I CTNAG 1 cut(s) 389
Hsp92II CATG 3 cut(s) 221, 449, 592
HspAI GCGC 2 cut(s) 226, 363
Kzo9I GATC 3 cut(s) 377, 557, 585
Lsp1109I GCAGC 1 cut(s) 431
LweI GCATC 1 cut(s) 467
MaeI CTAG 1 cut(s) 462
MaeIII GTNAC 3 cut(s) 95, 117, 529
MalI GATC 3 cut(s) 379, 559, 587
MboI GATC 3 cut(s) 377, 557, 585
MboII GAAGA 8 cut(s) 118, 121, 212, 275, 296, 477, 482, 488
MflI RGATCY 1 cut(s) 557
MhlI GDGCHC 3 cut(s) 29, 430, 506
MluCI AATT 3 cut(s) 204, 326, 470
MlyI GAGTC 1 cut(s) 311
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 5 cut(s) 41, 83, 86, 265, 569
MseI TTAA 2 cut(s) 114, 347
MslI CAYNNNNRTG 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 539
MspCI CTTAAG 1 cut(s) 346
MspI CCGG 1 cut(s) 407
MspR9I CCNGG 3 cut(s) 58, 407, 408
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 3 cut(s) 369, 425, 434
NciI CCSGG 2 cut(s) 407, 408
NdeII GATC 3 cut(s) 377, 557, 585
NlaIII CATG 3 cut(s) 221, 449, 592
NlaIV GGNNCC 2 cut(s) 28, 404
PagI TCATGA 1 cut(s) 217
PaqCI CACCTGC 1 cut(s) 413
PfeI GAWTC 1 cut(s) 240
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 1 cut(s) 421
PleI GAGTC 1 cut(s) 310
PpsI GAGTC 1 cut(s) 310
Psp124BI GAGCTC 1 cut(s) 506
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 2 cut(s) 28, 404
PsuI RGATCY 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 4 cut(s) 62, 316, 444, 564
RsaNI GTAC 4 cut(s) 61, 315, 443, 563
RseI CAYNNNNRTG 1 cut(s) 149
SacI GAGCTC 1 cut(s) 506
SaqAI TTAA 2 cut(s) 114, 347
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 3 cut(s) 377, 557, 585
ScaI AGTACT 1 cut(s) 316
SchI GAGTC 1 cut(s) 311
ScrFI CCNGG 3 cut(s) 58, 407, 408
SduI GDGCHC 3 cut(s) 29, 430, 506
SetI ASST 9 cut(s) 97, 124, 137, 198, 395, 427, 506, 524, 541
SfaNI GCATC 1 cut(s) 467
SmaI CCCGGG 1 cut(s) 408
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 3 cut(s) 204, 326, 470
SsiI CCGC 1 cut(s) 351
SspMI CTAG 1 cut(s) 462
SstI GAGCTC 1 cut(s) 506
StyD4I CCNGG 3 cut(s) 56, 405, 406
StyI CCWWGG 1 cut(s) 135
TaqI TCGA 2 cut(s) 41, 300
TasI AATT 3 cut(s) 204, 326, 470
TatI WGTACW 2 cut(s) 314, 562
TfiI GAWTC 1 cut(s) 240
Tru1I TTAA 2 cut(s) 114, 347
Tru9I TTAA 2 cut(s) 114, 347
TscAI CASTG 2 cut(s) 51, 255
TseI GCWGC 1 cut(s) 419
TspDTI ATGAA 3 cut(s) 206, 462, 482
TspGWI ACGGA 1 cut(s) 430
TspMI CCCGGG 1 cut(s) 406
TspRI CASTG 2 cut(s) 51, 255
Van91I CCANNNNNTGG 1 cut(s) 250
Vha464I CTTAAG 1 cut(s) 346
XapI RAATTY 1 cut(s) 326
XbaI TCTAGA 1 cut(s) 461
XmaI CCCGGG 1 cut(s) 406
XspI CTAG 1 cut(s) 462
ZrmI AGTACT 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.