Rw2G001250
ERF Family

Calcineurin-like metallo-phosphoesterase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010859
Physical Location & Seq
Forward (+)
1047027 .. 1049709
2683 bp
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UTR
Exon/CDS
Intron
Rw2G001250.1

Sequence Viewer

Length: 882 bp
ATGGAGAAGGAACGTTTATGGGTTCCCACTGTTGTGGCACAAGTGTGTCTCTGCTTTGGTCTCTACCTTGCTTTTCAACTGGGTCAGCCTCAGAACTCTGTGAACCTCCATAGAAGAAGTGAAAGCAGAGATCTTTACTTCATCAGCGTCAGAGGAGGCTTCAGACCTCTCAAGCAACAGACCCATCTTCTCAAACAGATGGAGAAGGTGGCAAAGAAGTACAAGGCAAAGTTTGTAGTGGACATCAGTGACCTTGGGAAAGATGATCCGCTCATGCAGAATGGTACCCTGCATTTCTCATCATTAAAGGTTCCCTGGTATACTACTACAGTTTCAGGCAGATATGGAGGGGGTTACTTCCAGAAGCAGATAAGACTACCTTCCAAGAAAACCTTAGACCTCATTGGTGTGGATACTGGGTCATTAGAGGGGAAGGTTTTAATCTCGGGATCATCAAGTGAGTTTGGATTCAATCAGTTACGTTGGCTGACAAAGACACTAGAGGAAACCAGTAGTGACTGGTGCATAGTTGTTGGATTCCACCCATTGGCTAAATGTGAAGATAGTGAAGAGGGAATGGAAGCAAACCAAGTCTTTGAGTCTCTACATCATAGTTTTATGAAATATGAAGTGAATGCATATATAAGTGGGCAGGGTTGCACTAGCGATGTCCAACAAGGTACTCTGAGCTACATTGGGAACCCCGGCTCAACAAATGCACTAGCTTTTGTAAATGAGACATCAATATTTAGTAGAGAACTGGAGGTCGAGTTTCTTCTTCACAGAGTCAGCTCGCTAGAAATTGTAACCTACTTTGTTAGCTTGAATGGGGAAGTAGTGCATAAATCTGTACTCCATCAGAAGGGTAAAGCAGTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

32.92

Weight (kDa)

7.67

Isoelectric Point (pI)

29.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30993 AT4G30993 AT4G30993
fragaria_vesca FvH4_1g01190 FvH4_1g01190
malus_domestica MD02G1010000.v1.1 MD02G1010100.v1.1
prunus_persica Prupe.7G260000_v2.0.a1
pyrus_communis pycom02g00880 pycom15g13910
rosa_chinensis RchiOBHm_Chr2g0085991
rosa_laevigata RLG00000015732
rosa_multiflora Rmu_ssc0000355.1_g000024
rosa_roxburghii Rroxscaffold_2G00154870
rosa_rugosa Rorug01G0464900
rosa_samantha Rh2AG014100 Rh2BG014600 Rh2CG015100 Rh2DG016400
rosa_wichuraiana Rw2G001250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 284
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 1 cut(s) 271
AccI GTMKAC 1 cut(s) 320
AciI CCGC 1 cut(s) 269
AclI AACGTT 1 cut(s) 13
AclWI GGATC 2 cut(s) 260, 457
AcuI CTGAAG 1 cut(s) 145
AfaI GTAC 4 cut(s) 221, 286, 682, 852
AfiI CCNNNNNNNGG 2 cut(s) 547, 862
AgsI TTSAA 3 cut(s) 77, 472, 826
AjnI CCWGG 1 cut(s) 314
AleI CACNNNNGTG 2 cut(s) 32, 43
AluBI AGCT 4 cut(s) 690, 725, 792, 822
AluI AGCT 4 cut(s) 690, 725, 792, 822
Alw26I GTCTC 4 cut(s) 53, 65, 606, 731
AlwI GGATC 2 cut(s) 260, 457
Ama87I CYCGRG 1 cut(s) 445
Asp718I GGTACC 1 cut(s) 284
AsuC2I CCSGG 1 cut(s) 705
AvaI CYCGRG 1 cut(s) 445
BanI GGYRCC 1 cut(s) 284
BccI CCATC 3 cut(s) 192, 193, 864
BciT130I CCWGG 1 cut(s) 316
BciVI GTATCC 1 cut(s) 406
BcnI CCSGG 1 cut(s) 705
BcoDI GTCTC 4 cut(s) 53, 65, 606, 731
BfaI CTAG 4 cut(s) 500, 663, 722, 797
BfmI CTRYAG 1 cut(s) 327
BfuI GTATCC 1 cut(s) 406
BglII AGATCT 1 cut(s) 130
Bme1390I CCNGG 2 cut(s) 316, 705
BmeT110I CYCGRG 1 cut(s) 445
BmiI GGNNCC 4 cut(s) 24, 286, 312, 701
BmrFI CCNGG 2 cut(s) 316, 705
BmrI ACTGGG 2 cut(s) 89, 426
BmuI ACTGGG 2 cut(s) 89, 426
BpmI CTGGAG 1 cut(s) 782
BpuEI CTTGAG 1 cut(s) 155
BpuMI CCSGG 1 cut(s) 705
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 3 cut(s) 253, 314, 703
BsaXI ACNNNNNCTCC 2 cut(s) 755, 785
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 862
Bse1I ACTGG 5 cut(s) 84, 421, 510, 524, 765
BseBI CCWGG 1 cut(s) 316
BseDI CCNNGG 3 cut(s) 253, 314, 703
BseLI CCNNNNNNNGG 2 cut(s) 547, 862
BseMII CTCAG 2 cut(s) 104, 677
BseNI ACTGG 5 cut(s) 84, 421, 510, 524, 765
BseRI GAGGAG 1 cut(s) 168
BshNI GGYRCC 1 cut(s) 284
BsiHKCI CYCGRG 1 cut(s) 445
BsiSI CCGG 1 cut(s) 705
BslI CCNNNNNNNGG 2 cut(s) 547, 862
BsmAI GTCTC 4 cut(s) 53, 65, 606, 731
BsmI GAATGC 1 cut(s) 640
Bso31I GGTCTC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 445
Bsp143I GATC 3 cut(s) 130, 265, 449
BspACI CCGC 1 cut(s) 269
BspCNI CTCAG 2 cut(s) 103, 678
BspLI GGNNCC 4 cut(s) 24, 286, 312, 701
BspPI GGATC 2 cut(s) 260, 457
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 271
BsrI ACTGG 5 cut(s) 84, 421, 510, 524, 765
BssECI CCNNGG 3 cut(s) 253, 314, 703
BssMI GATC 3 cut(s) 130, 265, 449
BssNAI GTATAC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 253
Bst1107I GTATAC 1 cut(s) 321
Bst2UI CCWGG 1 cut(s) 316
Bst4CI ACNGT 2 cut(s) 31, 331
Bst6I CTCTTC 1 cut(s) 564
BstC8I GCNNGC 1 cut(s) 794
BstDEI CTNAG 3 cut(s) 90, 394, 686
BstKTI GATC 3 cut(s) 133, 268, 452
BstMAI GTCTC 4 cut(s) 53, 65, 606, 731
BstMBI GATC 3 cut(s) 130, 265, 449
BstNI CCWGG 1 cut(s) 316
BstSCI CCNGG 2 cut(s) 314, 703
BstSFI CTRYAG 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 130
BstXI CCANNNNNNTGG 1 cut(s) 34
BstYI RGATCY 1 cut(s) 130
BstZ17I GTATAC 1 cut(s) 321
BsuI GTATCC 1 cut(s) 406
BtgZI GCGATG 1 cut(s) 681
BtsIMutI CAGTG 2 cut(s) 27, 253
Cac8I GCNNGC 1 cut(s) 794
CseI GACGC 1 cut(s) 136
Csp6I GTAC 4 cut(s) 220, 285, 681, 851
CviAII CATG 2 cut(s) 274, 877
CviJI RGCY 9 cut(s) 88, 159, 487, 551, 690, 708, 725, 792, 822
CviKI_1 RGCY 9 cut(s) 88, 159, 487, 551, 690, 708, 725, 792, 822
CviQI GTAC 4 cut(s) 220, 285, 681, 851
DdeI CTNAG 3 cut(s) 90, 394, 686
DpnI GATC 3 cut(s) 132, 267, 451
DpnII GATC 3 cut(s) 130, 265, 449
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 253
EcoT22I ATGCAT 1 cut(s) 640
ErhI CCWWGG 1 cut(s) 253
FaeI CATG 2 cut(s) 277, 880
FalI AAGNNNNNCTT 4 cut(s) 364, 396, 377, 409
FatI CATG 2 cut(s) 273, 876
FblI GTMKAC 1 cut(s) 320
FspBI CTAG 4 cut(s) 500, 663, 722, 797
GsuI CTGGAG 1 cut(s) 782
HapII CCGG 1 cut(s) 705
HgaI GACGC 1 cut(s) 136
Hin1II CATG 2 cut(s) 277, 880
HinfI GANTC 4 cut(s) 468, 537, 599, 786
HpaII CCGG 1 cut(s) 705
Hpy166II GTNNAC 3 cut(s) 103, 241, 321
Hpy188I TCNGA 5 cut(s) 93, 152, 164, 687, 861
Hpy188III TCNNGA 2 cut(s) 361, 447
Hpy8I GTNNAC 3 cut(s) 103, 241, 321
HpyAV CCTTC 4 cut(s) 199, 390, 427, 856
HpyCH4III ACNGT 2 cut(s) 31, 331
HpyCH4IV ACGT 2 cut(s) 13, 481
HpyCH4V TGCA 7 cut(s) 277, 292, 525, 638, 660, 719, 841
HpyF3I CTNAG 3 cut(s) 90, 394, 686
HpySE526I ACGT 2 cut(s) 13, 481
Hsp92II CATG 2 cut(s) 277, 880
KpnI GGTACC 1 cut(s) 288
Kzo9I GATC 3 cut(s) 130, 265, 449
MaeI CTAG 4 cut(s) 500, 663, 722, 797
MaeII ACGT 2 cut(s) 13, 481
MaeIII GTNAC 5 cut(s) 248, 353, 477, 515, 805
MalI GATC 3 cut(s) 132, 267, 451
MbiI CCGCTC 1 cut(s) 271
MboI GATC 3 cut(s) 130, 265, 449
MboII GAAGA 6 cut(s) 126, 179, 572, 581, 767, 770
MflI RGATCY 1 cut(s) 130
MluCI AATT 1 cut(s) 801
MlyI GAGTC 2 cut(s) 608, 795
MmeI TCCRAC 2 cut(s) 514, 697
Mph1103I ATGCAT 1 cut(s) 640
MseI TTAA 2 cut(s) 305, 440
MslI CAYNNNNRTG 3 cut(s) 32, 43, 407
MspI CCGG 1 cut(s) 705
MspR9I CCNGG 2 cut(s) 316, 705
Mva1269I GAATGC 1 cut(s) 640
MvaI CCWGG 1 cut(s) 316
NciI CCSGG 1 cut(s) 705
NdeII GATC 3 cut(s) 130, 265, 449
NlaIII CATG 2 cut(s) 277, 880
NlaIV GGNNCC 4 cut(s) 24, 286, 312, 701
NmuCI GTSAC 2 cut(s) 248, 515
NsiI ATGCAT 1 cut(s) 640
OliI CACNNNNGTG 2 cut(s) 32, 43
PctI GAATGC 1 cut(s) 640
PfeI GAWTC 2 cut(s) 468, 537
PflMI CCANNNNNTGG 1 cut(s) 547
PleI GAGTC 2 cut(s) 607, 794
PpsI GAGTC 2 cut(s) 607, 794
Psp1406I AACGTT 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 314
PspGI CCWGG 1 cut(s) 314
PspN4I GGNNCC 4 cut(s) 24, 286, 312, 701
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 4 cut(s) 221, 286, 682, 852
RsaNI GTAC 4 cut(s) 220, 285, 681, 851
RseI CAYNNNNRTG 3 cut(s) 32, 43, 407
SaqAI TTAA 2 cut(s) 305, 440
Sau3AI GATC 3 cut(s) 130, 265, 449
SchI GAGTC 2 cut(s) 608, 795
ScrFI CCNGG 2 cut(s) 316, 705
SfcI CTRYAG 1 cut(s) 327
SmiMI CAYNNNNRTG 3 cut(s) 32, 43, 407
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 1 cut(s) 801
SsiI CCGC 1 cut(s) 269
SspI AATATT 1 cut(s) 747
SspMI CTAG 4 cut(s) 500, 663, 722, 797
StyD4I CCNGG 2 cut(s) 314, 703
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 2 cut(s) 31, 331
TaiI ACGT 2 cut(s) 16, 484
TaqI TCGA 1 cut(s) 768
TasI AATT 1 cut(s) 801
TatI WGTACW 2 cut(s) 219, 850
TfiI GAWTC 2 cut(s) 468, 537
Tru1I TTAA 2 cut(s) 305, 440
Tru9I TTAA 2 cut(s) 305, 440
TscAI CASTG 2 cut(s) 34, 253
TseFI GTSAC 2 cut(s) 248, 515
Tsp45I GTSAC 2 cut(s) 248, 515
TspDTI ATGAA 3 cut(s) 130, 635, 642
TspRI CASTG 2 cut(s) 34, 253
Van91I CCANNNNNTGG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 4 cut(s) 500, 663, 722, 797
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.