Rroxscaffold_2G00154870
ERF Family

Calcineurin-like metallo-phosphoesterase superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0010859
Physical Location & Seq
Reverse (-)
91339704 .. 91343073
3370 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154870.1

Sequence Viewer

Length: 882 bp
ATGGAGAAGAAACGTTTATGGGTTCCCACTATTGTGGCACAAGTGTGTCTCTGCTTTGGTCTCTACCTTGCTTTTCAACTGGGTCAGCCTCAGAACTCTGTGAACCTCCATAGAAGAAGTGAAAGCAGAGATCTTTACTTCATCAGCGTTAGAGGAGGCTTCAGACCTCTCAAGCAACAGACCCATCTTCTCAAACAGATGGAGAAGGTGGCAAAGAAGTACAAGGCAAAGTTTGTAGTGGACATCAGTGATCTTGGGAAAGATGATCCGCTCATGCAGAATGGTACCCTGCATTTCTCATCATTAAAGGTTCCTTGGTATACTACTACAGTTTCAGGCAGATATCGAGGGGGTTACTTCCAGAAGCAGATAAGACTACCTTACAAGAAAACCTTAGCCCTCATTGGTGTGGACACTGGGTCATTAGAGGGGAAGGTTTTAATCTCGGGATCATCAAGTGAGTTTGGATTCAATCAGTTACATTGGCTGACAAGGACGCTAGAGGAAACCAGTAGTGACTGGTGCATAGTTGTTGGATTCCACCCATTGGCTAAATGTGAAGATAGTGAAGAGGGAATGGAAGCAAAGCAAGTCTTTGAGACTCTACATCATAGTTTTATGAAATATGAAGTGAATGCATATATAAGTGAGCGGGGTTGCACTAGCGATGTCCAACAAGGTACTCTGAGCTACATTGGGAACCCGGGCTCAACAAATGCACTAGCTTTTGTAAATGAGACATCAATATTTAGTAGAGAACTGGAGGTCGAGTTTCTTCTTCACAGAGTCAGCTCGCTAGAAATTGTAACCTACTTTGTTAGCTTGAGTGGGGAAGTAGTGCATAAATCTGTACTCCATCAGAAGGGTAAAGCAGTCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

293

Amino Acids

33.18

Weight (kDa)

8.83

Isoelectric Point (pI)

26.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010859)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30993 AT4G30993 AT4G30993
fragaria_vesca FvH4_1g01190 FvH4_1g01190
malus_domestica MD02G1010000.v1.1 MD02G1010100.v1.1
prunus_persica Prupe.7G260000_v2.0.a1
pyrus_communis pycom02g00880 pycom15g13910
rosa_chinensis RchiOBHm_Chr2g0085991
rosa_laevigata RLG00000015732
rosa_multiflora Rmu_ssc0000355.1_g000024
rosa_roxburghii Rroxscaffold_2G00154870
rosa_rugosa Rorug01G0464900
rosa_samantha Rh2AG014100 Rh2BG014600 Rh2CG015100 Rh2DG016400
rosa_wichuraiana Rw2G001250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 284
AccB1I GGYRCC 1 cut(s) 284
AccB7I CCANNNNNTGG 1 cut(s) 547
AccBSI CCGCTC 2 cut(s) 271, 652
AccI GTMKAC 1 cut(s) 320
AciI CCGC 2 cut(s) 269, 652
AclI AACGTT 1 cut(s) 13
AclWI GGATC 2 cut(s) 260, 457
AcuI CTGAAG 1 cut(s) 145
AfaI GTAC 4 cut(s) 221, 286, 682, 852
AfiI CCNNNNNNNGG 2 cut(s) 547, 862
AgsI TTSAA 2 cut(s) 77, 472
AhdI GACNNNNNGTC 1 cut(s) 418
AleI CACNNNNGTG 2 cut(s) 32, 43
AluBI AGCT 4 cut(s) 690, 725, 792, 822
AluI AGCT 4 cut(s) 690, 725, 792, 822
Alw26I GTCTC 4 cut(s) 53, 65, 593, 731
AlwI GGATC 2 cut(s) 260, 457
Ama87I CYCGRG 2 cut(s) 445, 703
Asp718I GGTACC 1 cut(s) 284
AsuC2I CCSGG 2 cut(s) 704, 705
AvaI CYCGRG 2 cut(s) 445, 703
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 710
BccI CCATC 3 cut(s) 192, 193, 864
BcnI CCSGG 2 cut(s) 704, 705
BcoDI GTCTC 4 cut(s) 53, 65, 593, 731
BfaI CTAG 4 cut(s) 500, 663, 722, 797
BfmI CTRYAG 1 cut(s) 327
BglII AGATCT 1 cut(s) 130
Bme1390I CCNGG 2 cut(s) 704, 705
BmeRI GACNNNNNGTC 1 cut(s) 418
BmeT110I CYCGRG 2 cut(s) 445, 703
BmiI GGNNCC 4 cut(s) 24, 286, 312, 701
BmrFI CCNGG 2 cut(s) 704, 705
BmrI ACTGGG 2 cut(s) 89, 426
BmuI ACTGGG 2 cut(s) 89, 426
BpmI CTGGAG 1 cut(s) 782
Bpu10I CCTNAGC 1 cut(s) 394
BpuEI CTTGAG 2 cut(s) 155, 844
BpuMI CCSGG 2 cut(s) 704, 705
BsaI GGTCTC 1 cut(s) 65
BsaJI CCNNGG 2 cut(s) 314, 703
BsaXI ACNNNNNCTCC 2 cut(s) 755, 785
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 862
Bse1I ACTGG 5 cut(s) 84, 421, 510, 524, 765
BseDI CCNNGG 2 cut(s) 314, 703
BseLI CCNNNNNNNGG 2 cut(s) 547, 862
BseMII CTCAG 2 cut(s) 104, 677
BseNI ACTGG 5 cut(s) 84, 421, 510, 524, 765
BseRI GAGGAG 1 cut(s) 168
BshNI GGYRCC 1 cut(s) 284
BsiHKCI CYCGRG 2 cut(s) 445, 703
BsiSI CCGG 1 cut(s) 704
BslI CCNNNNNNNGG 2 cut(s) 547, 862
BsmAI GTCTC 4 cut(s) 53, 65, 593, 731
BsmI GAATGC 1 cut(s) 640
Bso31I GGTCTC 1 cut(s) 65
BsoBI CYCGRG 2 cut(s) 445, 703
Bsp1286I GDGCHC 1 cut(s) 710
Bsp143I GATC 4 cut(s) 130, 250, 265, 449
BspACI CCGC 2 cut(s) 269, 652
BspCNI CTCAG 2 cut(s) 103, 678
BspLI GGNNCC 4 cut(s) 24, 286, 312, 701
BspPI GGATC 2 cut(s) 260, 457
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 65
BsrBI CCGCTC 2 cut(s) 271, 652
BsrI ACTGG 5 cut(s) 84, 421, 510, 524, 765
BssECI CCNNGG 2 cut(s) 314, 703
BssMI GATC 4 cut(s) 130, 250, 265, 449
BssNAI GTATAC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 314
Bst1107I GTATAC 1 cut(s) 321
Bst4CI ACNGT 1 cut(s) 331
Bst6I CTCTTC 1 cut(s) 564
BstC8I GCNNGC 1 cut(s) 794
BstDEI CTNAG 3 cut(s) 90, 394, 686
BstKTI GATC 4 cut(s) 133, 253, 268, 452
BstMAI GTCTC 4 cut(s) 53, 65, 593, 731
BstMBI GATC 4 cut(s) 130, 250, 265, 449
BstSCI CCNGG 2 cut(s) 702, 703
BstSFI CTRYAG 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 130
BstXI CCANNNNNNTGG 1 cut(s) 34
BstYI RGATCY 1 cut(s) 130
BstZ17I GTATAC 1 cut(s) 321
BtgZI GCGATG 1 cut(s) 681
BtsIMutI CAGTG 2 cut(s) 253, 414
Cac8I GCNNGC 1 cut(s) 794
Cfr9I CCCGGG 1 cut(s) 703
CseI GACGC 1 cut(s) 505
Csp6I GTAC 4 cut(s) 220, 285, 681, 851
CviAII CATG 2 cut(s) 274, 877
CviQI GTAC 4 cut(s) 220, 285, 681, 851
DdeI CTNAG 3 cut(s) 90, 394, 686
DpnI GATC 4 cut(s) 132, 252, 267, 451
DpnII GATC 4 cut(s) 130, 250, 265, 449
DriI GACNNNNNGTC 1 cut(s) 418
Eam1104I CTCTTC 1 cut(s) 564
Eam1105I GACNNNNNGTC 1 cut(s) 418
EarI CTCTTC 1 cut(s) 564
Eco130I CCWWGG 1 cut(s) 314
Eco24I GRGCYC 1 cut(s) 710
Eco31I GGTCTC 1 cut(s) 65
Eco32I GATATC 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 145
Eco88I CYCGRG 2 cut(s) 445, 703
EcoRV GATATC 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 314
EcoT22I ATGCAT 1 cut(s) 640
EcoT38I GRGCYC 1 cut(s) 710
ErhI CCWWGG 1 cut(s) 314
FaeI CATG 2 cut(s) 277, 880
FalI AAGNNNNNCTT 6 cut(s) 364, 396, 377, 409, 578, 610
FatI CATG 2 cut(s) 273, 876
FauI CCCGC 1 cut(s) 645
FblI GTMKAC 1 cut(s) 320
FriOI GRGCYC 1 cut(s) 710
FspBI CTAG 4 cut(s) 500, 663, 722, 797
GsuI CTGGAG 1 cut(s) 782
HapII CCGG 1 cut(s) 704
HgaI GACGC 1 cut(s) 505
Hin1II CATG 2 cut(s) 277, 880
HinfI GANTC 4 cut(s) 468, 537, 601, 786
HpaII CCGG 1 cut(s) 704
Hpy166II GTNNAC 4 cut(s) 103, 241, 321, 412
Hpy188I TCNGA 4 cut(s) 93, 164, 687, 861
Hpy188III TCNNGA 2 cut(s) 361, 447
Hpy8I GTNNAC 4 cut(s) 103, 241, 321, 412
HpyAV CCTTC 3 cut(s) 199, 427, 856
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 1 cut(s) 13
HpyCH4V TGCA 7 cut(s) 277, 292, 525, 638, 660, 719, 841
HpyF3I CTNAG 3 cut(s) 90, 394, 686
HpySE526I ACGT 1 cut(s) 13
Hsp92II CATG 2 cut(s) 277, 880
KpnI GGTACC 1 cut(s) 288
Kzo9I GATC 4 cut(s) 130, 250, 265, 449
LpnPI CCDG 9 cut(s) 65, 302, 321, 374, 402, 505, 523, 717, 746
MaeI CTAG 4 cut(s) 500, 663, 722, 797
MaeII ACGT 1 cut(s) 13
MaeIII GTNAC 4 cut(s) 353, 477, 515, 805
MalI GATC 4 cut(s) 132, 252, 267, 451
MbiI CCGCTC 2 cut(s) 271, 652
MboI GATC 4 cut(s) 130, 250, 265, 449
MboII GAAGA 7 cut(s) 19, 126, 179, 572, 581, 767, 770
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 1 cut(s) 710
MluCI AATT 1 cut(s) 801
MlyI GAGTC 2 cut(s) 595, 795
MmeI TCCRAC 2 cut(s) 514, 697
Mph1103I ATGCAT 1 cut(s) 640
MseI TTAA 2 cut(s) 305, 440
MslI CAYNNNNRTG 3 cut(s) 32, 43, 407
MspI CCGG 1 cut(s) 704
MspR9I CCNGG 2 cut(s) 704, 705
Mva1269I GAATGC 1 cut(s) 640
NciI CCSGG 2 cut(s) 704, 705
NdeII GATC 4 cut(s) 130, 250, 265, 449
NlaIII CATG 2 cut(s) 277, 880
NlaIV GGNNCC 4 cut(s) 24, 286, 312, 701
NmuCI GTSAC 1 cut(s) 515
NsiI ATGCAT 1 cut(s) 640
OliI CACNNNNGTG 2 cut(s) 32, 43
PctI GAATGC 1 cut(s) 640
PfeI GAWTC 2 cut(s) 468, 537
PflMI CCANNNNNTGG 1 cut(s) 547
PleI GAGTC 2 cut(s) 595, 794
PpsI GAGTC 2 cut(s) 595, 794
Psp1406I AACGTT 1 cut(s) 13
PspN4I GGNNCC 4 cut(s) 24, 286, 312, 701
PsuI RGATCY 1 cut(s) 130
RsaI GTAC 4 cut(s) 221, 286, 682, 852
RsaNI GTAC 4 cut(s) 220, 285, 681, 851
RseI CAYNNNNRTG 3 cut(s) 32, 43, 407
SaqAI TTAA 2 cut(s) 305, 440
Sau3AI GATC 4 cut(s) 130, 250, 265, 449
SchI GAGTC 2 cut(s) 595, 795
ScrFI CCNGG 2 cut(s) 704, 705
SduI GDGCHC 1 cut(s) 710
SfcI CTRYAG 1 cut(s) 327
SmaI CCCGGG 1 cut(s) 705
SmiMI CAYNNNNRTG 3 cut(s) 32, 43, 407
SmlI CTYRAG 2 cut(s) 170, 823
SmoI CTYRAG 2 cut(s) 170, 823
Sse9I AATT 1 cut(s) 801
SsiI CCGC 2 cut(s) 269, 652
SspI AATATT 1 cut(s) 747
SspMI CTAG 4 cut(s) 500, 663, 722, 797
StyD4I CCNGG 2 cut(s) 702, 703
StyI CCWWGG 1 cut(s) 314
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 1 cut(s) 16
TaqI TCGA 2 cut(s) 346, 768
TasI AATT 1 cut(s) 801
TatI WGTACW 2 cut(s) 219, 850
TfiI GAWTC 2 cut(s) 468, 537
Tru1I TTAA 2 cut(s) 305, 440
Tru9I TTAA 2 cut(s) 305, 440
TscAI CASTG 2 cut(s) 253, 421
TseFI GTSAC 1 cut(s) 515
Tsp45I GTSAC 1 cut(s) 515
TspDTI ATGAA 3 cut(s) 130, 635, 642
TspMI CCCGGG 1 cut(s) 703
TspRI CASTG 2 cut(s) 253, 421
Van91I CCANNNNNTGG 1 cut(s) 547
XmaI CCCGGG 1 cut(s) 703
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 4 cut(s) 500, 663, 722, 797
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.