AT4G32590

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0011017
Physical Location & Seq
Forward (+)
15721289 .. 15722946
1658 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32590.5

Sequence Viewer

Length: 522 bp
ATGGCTTCGTTAGGTTTCAACCTCGGCTTCACCTTCTCCAATGCACAAATTCAGCAGCATCGAAAAGTTTCCGGAGGTGGAAGAGCTCGCGTAATCTCCTGTAACAGCTCTTCTTCTTCTTCTTCTCAAGCATCATCTCCTCAGGGGATTTCTGCAGTGACGCCTCTTGAGATAGAGCTCGAGTTCTTCGGTCCGAAGCCGGGTAGCGACGGGTCATATCCGGTAGATAAAGCAAAGGCTGTGAGTGGAGATAAGCTTCTCAGAAGCATTATGCAGGATAACAAAATTGAACTCTACGCTGCTTACGGGAAAGTGATGAACTGTGGTGGTGGAGGAAGCTGTGGAACTTGCATTGTGGAGATACTCGATGGCAGAGATCTTCTGAATGAGAGAACAGATACAGAAAACCGGTACCTGAAAAAGAAGCCGGAATCTTGGAGACTCGCATGTCAAACAATAGTAGGGAACAAAGAAAACTCAGGAAAGGTTGTCGTGCAGCGGATTCCACAGTGGAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

173

Amino Acids

18.66

Weight (kDa)

9.26

Isoelectric Point (pI)

45.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32590 AT4G32590 AT4G32590 AT4G32590 AT4G32590
fragaria_vesca FvH4_2g30090 FvH4_2g30090
malus_domestica MD15G1116000.v1.1
prunus_persica Prupe.1G467300_v2.0.a1
pyrus_communis pycom15g10460
rosa_chinensis RchiOBHm_Chr6g0312831
rosa_laevigata RLG00000010294
rosa_multiflora Rmu_co8502815.1_g000001
rosa_roxburghii Rroxscaffold_7G00156450
rosa_rugosa Rorug06G0399900
rosa_samantha Rh6AG517100 Rh6BG528500 Rh6CG533200 Rh6DG519700
rosa_wichuraiana Rw6G044920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 447
Acc65I GGTACC 1 cut(s) 411
AccB1I GGYRCC 1 cut(s) 411
AccII CGCG 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 71
AciI CCGC 1 cut(s) 499
AcsI RAATTY 1 cut(s) 48
AcyI GRCGYC 1 cut(s) 161
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 1 cut(s) 200
AgeI ACCGGT 1 cut(s) 408
AgsI TTSAA 2 cut(s) 19, 290
AluBI AGCT 5 cut(s) 86, 108, 178, 256, 339
AluI AGCT 5 cut(s) 86, 108, 178, 256, 339
Alw21I GWGCWC 2 cut(s) 88, 180
Alw26I GTCTC 1 cut(s) 433
Ama87I CYCGRG 1 cut(s) 179
Aor13HI TCCGGA 1 cut(s) 71
ApeKI GCWGC 3 cut(s) 55, 299, 496
ApoI RAATTY 1 cut(s) 48
AsiGI ACCGGT 1 cut(s) 408
Asp700I GAANNNNTTC 1 cut(s) 67
Asp718I GGTACC 1 cut(s) 411
AspS9I GGNCC 1 cut(s) 191
AsuC2I CCSGG 1 cut(s) 201
AsuHPI GGTGA 1 cut(s) 22
AvaI CYCGRG 1 cut(s) 179
AvaII GGWCC 1 cut(s) 191
AxyI CCTNAGG 1 cut(s) 141
BanI GGYRCC 1 cut(s) 411
BanII GRGCYC 2 cut(s) 88, 180
BarI GAAGNNNNNNTAC 2 cut(s) 94, 126
Bbv12I GWGCWC 2 cut(s) 88, 180
BbvI GCAGC 3 cut(s) 67, 286, 508
BccI CCATC 1 cut(s) 362
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 433
BfmI CTRYAG 1 cut(s) 153
BglII AGATCT 1 cut(s) 376
BisI GCNGC 3 cut(s) 56, 300, 497
BlsI GCNGC 3 cut(s) 57, 301, 498
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 1 cut(s) 191
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 1 cut(s) 413
BmrFI CCNGG 1 cut(s) 201
BmsI GCATC 2 cut(s) 67, 140
BpuEI CTTGAG 2 cut(s) 111, 188
BpuMI CCSGG 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 22
BsaWI WCCGGW 3 cut(s) 71, 220, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 408
Bse21I CCTNAGG 1 cut(s) 141
BseAI TCCGGA 1 cut(s) 71
BseDI CCNNGG 1 cut(s) 22
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMII CTCAG 3 cut(s) 155, 274, 492
BseRI GAGGAG 1 cut(s) 129
BseXI GCAGC 3 cut(s) 67, 286, 508
BsgI GTGCAG 1 cut(s) 515
Bsh1236I CGCG 1 cut(s) 90
BshNI GGYRCC 1 cut(s) 411
BshTI ACCGGT 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 88, 180
BsiHKCI CYCGRG 1 cut(s) 179
BsiSI CCGG 5 cut(s) 72, 200, 221, 409, 428
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 1 cut(s) 433
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 88, 180
Bsp13I TCCGGA 1 cut(s) 71
Bsp143I GATC 1 cut(s) 376
BspACI CCGC 1 cut(s) 499
BspCNI CTCAG 3 cut(s) 154, 273, 491
BspEI TCCGGA 1 cut(s) 71
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 413
BspMAI CTGCAG 1 cut(s) 157
BspQI GCTCTTC 2 cut(s) 76, 115
BspT107I GGYRCC 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 408
BssAI RCCGGY 1 cut(s) 408
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 376
BssNI GRCGYC 1 cut(s) 161
Bst4CI ACNGT 2 cut(s) 323, 510
Bst6I CTCTTC 2 cut(s) 76, 115
BstACI GRCGYC 1 cut(s) 161
BstC8I GCNNGC 1 cut(s) 88
BstDEI CTNAG 3 cut(s) 141, 260, 478
BstFNI CGCG 1 cut(s) 90
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 1 cut(s) 433
BstMBI GATC 1 cut(s) 376
BstNSI RCATGY 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 199
BstSFI CTRYAG 1 cut(s) 153
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 67, 286, 508
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
Bsu36I CCTNAGG 1 cut(s) 141
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 162, 515
Cac8I GCNNGC 1 cut(s) 88
Cfr10I RCCGGY 1 cut(s) 408
Cfr13I GGNCC 1 cut(s) 191
CpoI CGGWCCG 1 cut(s) 191
CseI GACGC 1 cut(s) 169
Csp6I GTAC 1 cut(s) 412
CspAI ACCGGT 1 cut(s) 408
CspI CGGWCCG 1 cut(s) 191
CviAII CATG 1 cut(s) 447
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 3 cut(s) 141, 260, 478
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
DrdI GACNNNNNNGTC 1 cut(s) 447
DseDI GACNNNNNNGTC 1 cut(s) 447
Eam1104I CTCTTC 2 cut(s) 76, 115
EarI CTCTTC 2 cut(s) 76, 115
Ecl136II GAGCTC 2 cut(s) 86, 178
Eco24I GRGCYC 2 cut(s) 88, 180
Eco47I GGWCC 1 cut(s) 191
Eco53kI GAGCTC 2 cut(s) 86, 178
Eco81I CCTNAGG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 179
EcoICRI GAGCTC 2 cut(s) 86, 178
EcoT38I GRGCYC 2 cut(s) 88, 180
FaeI CATG 1 cut(s) 450
FaiI YATR 3 cut(s) 217, 272, 448
FatI CATG 1 cut(s) 446
Fnu4HI GCNGC 3 cut(s) 56, 300, 497
FriOI GRGCYC 2 cut(s) 88, 180
Fsp4HI GCNGC 3 cut(s) 56, 300, 497
GluI GCNGC 3 cut(s) 56, 300, 497
HapII CCGG 5 cut(s) 72, 200, 221, 409, 428
HgaI GACGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 161
Hin1II CATG 1 cut(s) 450
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 3 cut(s) 431, 441, 502
HpaII CCGG 5 cut(s) 72, 200, 221, 409, 428
HphI GGTGA 1 cut(s) 22
Hpy188I TCNGA 3 cut(s) 195, 263, 384
Hpy188III TCNNGA 3 cut(s) 72, 167, 480
Hpy99I CGWCG 1 cut(s) 212
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 2 cut(s) 323, 510
HpyCH4V TGCA 5 cut(s) 44, 155, 274, 351, 496
HpyF3I CTNAG 3 cut(s) 141, 260, 478
Hsp92I GRCGYC 1 cut(s) 161
Hsp92II CATG 1 cut(s) 450
Kpn2I TCCGGA 1 cut(s) 71
KpnI GGTACC 1 cut(s) 415
Kzo9I GATC 1 cut(s) 376
LguI GCTCTTC 2 cut(s) 76, 115
Lsp1109I GCAGC 3 cut(s) 67, 286, 508
LweI GCATC 2 cut(s) 67, 140
MaeIII GTNAC 2 cut(s) 101, 157
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 8 cut(s) 93, 102, 105, 108, 111, 114, 178, 371
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 2 cut(s) 88, 180
MluCI AATT 2 cut(s) 48, 285
MlyI GAGTC 1 cut(s) 435
MnlI CCTC 5 cut(s) 32, 68, 150, 174, 326
MroI TCCGGA 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 67
MspA1I CMGCKG 1 cut(s) 499
MspI CCGG 5 cut(s) 72, 200, 221, 409, 428
MspR9I CCNGG 1 cut(s) 201
MvnI CGCG 1 cut(s) 90
NciI CCSGG 1 cut(s) 201
NdeII GATC 1 cut(s) 376
NlaIII CATG 1 cut(s) 450
NlaIV GGNNCC 1 cut(s) 413
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 450
PaeR7I CTCGAG 1 cut(s) 179
PciSI GCTCTTC 2 cut(s) 76, 115
PdmI GAANNNNTTC 1 cut(s) 67
PfeI GAWTC 2 cut(s) 431, 502
PinAI ACCGGT 1 cut(s) 408
PkrI GCNGC 3 cut(s) 57, 301, 498
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
Psp124BI GAGCTC 2 cut(s) 88, 180
PspN4I GGNNCC 1 cut(s) 413
PspPI GGNCC 1 cut(s) 191
PspXI VCTCGAGB 1 cut(s) 179
PstI CTGCAG 1 cut(s) 157
PsuI RGATCY 1 cut(s) 376
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
Rsr2I CGGWCCG 1 cut(s) 191
RsrII CGGWCCG 1 cut(s) 191
SacI GAGCTC 2 cut(s) 88, 180
SapI GCTCTTC 2 cut(s) 76, 115
SatI GCNGC 3 cut(s) 56, 300, 497
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 1 cut(s) 201
SduI GDGCHC 2 cut(s) 88, 180
SfaNI GCATC 2 cut(s) 67, 140
SfcI CTRYAG 1 cut(s) 153
Sfr274I CTCGAG 1 cut(s) 179
SinI GGWCC 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 179
SmlI CTYRAG 3 cut(s) 126, 167, 179
SmoI CTYRAG 3 cut(s) 126, 167, 179
Sse9I AATT 2 cut(s) 48, 285
SsiI CCGC 1 cut(s) 499
SstI GAGCTC 2 cut(s) 88, 180
StyD4I CCNGG 1 cut(s) 199
TaaI ACNGT 2 cut(s) 323, 510
TaqI TCGA 3 cut(s) 61, 180, 366
TaqII GACCGA 1 cut(s) 179
TasI AATT 2 cut(s) 48, 285
TfiI GAWTC 2 cut(s) 431, 502
TscAI CASTG 2 cut(s) 162, 515
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 3 cut(s) 55, 299, 496
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 1 cut(s) 332
TspRI CASTG 2 cut(s) 162, 515
VpaK11BI GGWCC 1 cut(s) 191
XapI RAATTY 1 cut(s) 48
XceI RCATGY 1 cut(s) 450
XhoI CTCGAG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.