Rh6DG519700

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0011017
Physical Location & Seq
Reverse (-)
67000833 .. 67003442
2610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG519700.1

Sequence Viewer

Length: 492 bp
ATGGCGATGGCGGCTTGCATGTACTTCTCTAACGCCACGTCGTTCCGGCCAGTCCGATTCTCCGGCGCCGGAAATTACCAGAAGCCAACCAATTTTCTTGCCAGAAGGAAGTCGGTCTCCTGCGCGGGAAGTTCGCCGGAAATCGAGCTCGAATTCATAGCGCCGAAGCCGGCGAACGACGGGTCGTATGCGGTGGAGAAGGCGACGGCGATTAGTGGGGAGAAGCTCCTGAGGAGCATAATGTCGGATAACAAGATCCAGCTTTACGCCACGTATGGGAAGCTGATGAACTGTGGAGGTGTTGGAAGCTGTGGGACTTGCATTGTAGAGATTATTGATGGGAAGGATCTTTTGAATGAAAGAACGAATACTGAACTCAAATATTTGAAAAAGAAACCTGAATCTTGGAGACTAGCTTGCCAAACTATAGTGGGAAATAAAGAAAATTCTGGGAAGGTTCTTGTTCAGAGGATTCCCCAGTGGAAAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fer2

Protein Analysis

163

Amino Acids

17.94

Weight (kDa)

9.42

Isoelectric Point (pI)

44.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 96 - 144 1.4e-06 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011017)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32590 AT4G32590 AT4G32590 AT4G32590 AT4G32590
fragaria_vesca FvH4_2g30090 FvH4_2g30090
malus_domestica MD15G1116000.v1.1
prunus_persica Prupe.1G467300_v2.0.a1
pyrus_communis pycom15g10460
rosa_chinensis RchiOBHm_Chr6g0312831
rosa_laevigata RLG00000010294
rosa_multiflora Rmu_co8502815.1_g000001
rosa_roxburghii Rroxscaffold_7G00156450
rosa_rugosa Rorug06G0399900
rosa_samantha Rh6AG517100 Rh6BG528500 Rh6CG533200 Rh6DG519700
rosa_wichuraiana Rw6G044920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 65
AccII CGCG 1 cut(s) 125
AciI CCGC 3 cut(s) 11, 125, 191
AclWI GGATC 2 cut(s) 250, 354
AcoI YGGCCR 1 cut(s) 47
AcsI RAATTY 2 cut(s) 152, 445
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 1 cut(s) 23
AfiI CCNNNNNNNGG 1 cut(s) 276
AgsI TTSAA 2 cut(s) 355, 388
AjiI CACGTC 1 cut(s) 39
AluBI AGCT 6 cut(s) 148, 226, 262, 283, 309, 416
AluI AGCT 6 cut(s) 148, 226, 262, 283, 309, 416
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 2 cut(s) 121, 403
AlwI GGATC 2 cut(s) 250, 354
AoxI GGCC 1 cut(s) 47
ApoI RAATTY 2 cut(s) 152, 445
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
AspLEI GCGC 3 cut(s) 68, 125, 163
AxyI CCTNAGG 1 cut(s) 230
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 1 cut(s) 332
BceAI ACGGC 1 cut(s) 222
BcoDI GTCTC 2 cut(s) 121, 403
BfaI CTAG 1 cut(s) 413
BfmI CTRYAG 1 cut(s) 426
BfoI RGCGCY 2 cut(s) 69, 164
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
BmgBI CACGTC 1 cut(s) 39
BmiI GGNNCC 1 cut(s) 67
BmrI ACTGGG 1 cut(s) 472
BmuI ACTGGG 1 cut(s) 472
BsaAI YACGTR 1 cut(s) 273
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 121
Bsc4I CCNNNNNNNGG 1 cut(s) 276
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 50, 478
Bse21I CCTNAGG 1 cut(s) 230
BseLI CCNNNNNNNGG 1 cut(s) 276
BseMII CTCAG 1 cut(s) 221
BseNI ACTGG 2 cut(s) 50, 478
BseRI GAGGAG 1 cut(s) 247
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 1 cut(s) 49
BshNI GGYRCC 1 cut(s) 65
BsiHKAI GWGCWC 1 cut(s) 150
BsiSI CCGG 5 cut(s) 46, 63, 69, 137, 170
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 1 cut(s) 276
BsmAI GTCTC 2 cut(s) 121, 403
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 1 cut(s) 49
Bso31I GGTCTC 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 2 cut(s) 255, 346
BspACI CCGC 3 cut(s) 11, 125, 191
BspANI GGCC 1 cut(s) 49
BspCNI CTCAG 1 cut(s) 222
BspFNI CGCG 1 cut(s) 125
BspLI GGNNCC 1 cut(s) 67
BspPI GGATC 2 cut(s) 250, 354
BspT107I GGYRCC 1 cut(s) 65
BspTNI GGTCTC 1 cut(s) 121
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 2 cut(s) 50, 478
BssAI RCCGGY 1 cut(s) 169
BssMI GATC 2 cut(s) 255, 346
BssNI GRCGYC 1 cut(s) 66
Bst4CI ACNGT 1 cut(s) 293
BstACI GRCGYC 1 cut(s) 66
BstBAI YACGTR 1 cut(s) 273
BstC8I GCNNGC 3 cut(s) 16, 171, 418
BstDEI CTNAG 1 cut(s) 230
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 2 cut(s) 69, 164
BstHHI GCGC 3 cut(s) 68, 125, 163
BstKTI GATC 2 cut(s) 258, 349
BstMAI GTCTC 2 cut(s) 121, 403
BstMBI GATC 2 cut(s) 255, 346
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNSI RCATGY 1 cut(s) 22
BstSFI CTRYAG 1 cut(s) 426
BstUI CGCG 1 cut(s) 125
BstX2I RGATCY 2 cut(s) 255, 346
BstYI RGATCY 2 cut(s) 255, 346
Bsu36I CCTNAGG 1 cut(s) 230
BsuRI GGCC 1 cut(s) 49
BtgZI GCGATG 1 cut(s) 20
BtrI CACGTC 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 485
Cac8I GCNNGC 3 cut(s) 16, 171, 418
CfoI GCGC 3 cut(s) 68, 125, 163
Cfr10I RCCGGY 1 cut(s) 169
Csp6I GTAC 1 cut(s) 22
CviAII CATG 1 cut(s) 19
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 1 cut(s) 230
DinI GGCGCC 1 cut(s) 67
DpnI GATC 2 cut(s) 257, 348
DpnII GATC 2 cut(s) 255, 346
EaeI YGGCCR 1 cut(s) 47
Ecl136II GAGCTC 1 cut(s) 148
Eco24I GRGCYC 1 cut(s) 150
Eco31I GGTCTC 1 cut(s) 121
Eco53kI GAGCTC 1 cut(s) 148
Eco81I CCTNAGG 1 cut(s) 230
EcoICRI GAGCTC 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 152
EcoT38I GRGCYC 1 cut(s) 150
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
FaeI CATG 1 cut(s) 22
FaiI YATR 6 cut(s) 20, 158, 189, 239, 276, 428
FaqI GGGAC 1 cut(s) 328
FatI CATG 1 cut(s) 18
FauI CCCGC 1 cut(s) 118
Fnu4HI GCNGC 1 cut(s) 12
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 1 cut(s) 413
GlaI GCGC 3 cut(s) 67, 124, 162
GluI GCNGC 1 cut(s) 12
HaeII RGCGCY 2 cut(s) 69, 164
HaeIII GGCC 1 cut(s) 49
HapII CCGG 5 cut(s) 46, 63, 69, 137, 170
HhaI GCGC 3 cut(s) 68, 125, 163
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 1 cut(s) 22
Hin6I GCGC 3 cut(s) 66, 123, 161
HinP1I GCGC 3 cut(s) 66, 123, 161
HinfI GANTC 3 cut(s) 57, 401, 472
HpaII CCGG 5 cut(s) 46, 63, 69, 137, 170
Hpy188I TCNGA 3 cut(s) 56, 247, 468
Hpy188III TCNNGA 1 cut(s) 229
Hpy99I CGWCG 3 cut(s) 43, 182, 208
HpyAV CCTTC 4 cut(s) 99, 193, 337, 448
HpyCH4III ACNGT 1 cut(s) 293
HpyCH4IV ACGT 2 cut(s) 38, 272
HpyCH4V TGCA 2 cut(s) 18, 321
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 1 cut(s) 230
HpySE526I ACGT 2 cut(s) 38, 272
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 1 cut(s) 22
HspAI GCGC 3 cut(s) 66, 123, 161
KasI GGCGCC 1 cut(s) 65
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
Kzo9I GATC 2 cut(s) 255, 346
LmnI GCTCC 2 cut(s) 231, 234
MaeI CTAG 1 cut(s) 413
MaeII ACGT 2 cut(s) 38, 272
MalI GATC 2 cut(s) 257, 348
MboI GATC 2 cut(s) 255, 346
MflI RGATCY 2 cut(s) 255, 346
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 4 cut(s) 73, 91, 152, 445
Mly113I GGCGCC 1 cut(s) 66
MmeI TCCRAC 2 cut(s) 225, 283
MnlI CCTC 3 cut(s) 225, 290, 462
MroNI GCCGGC 1 cut(s) 169
MspI CCGG 5 cut(s) 46, 63, 69, 137, 170
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 1 cut(s) 11
NaeI GCCGGC 1 cut(s) 171
NarI GGCGCC 1 cut(s) 66
NdeII GATC 2 cut(s) 255, 346
NgoMIV GCCGGC 1 cut(s) 169
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 67
NspI RCATGY 1 cut(s) 22
PdiI GCCGGC 1 cut(s) 171
PfeI GAWTC 3 cut(s) 57, 401, 472
PkrI GCNGC 1 cut(s) 13
PluTI GGCGCC 1 cut(s) 69
Ppu21I YACGTR 1 cut(s) 273
Psp124BI GAGCTC 1 cut(s) 150
PspN4I GGNNCC 1 cut(s) 67
PsuI RGATCY 2 cut(s) 255, 346
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
SacI GAGCTC 1 cut(s) 150
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 2 cut(s) 255, 346
SduI GDGCHC 1 cut(s) 150
SfcI CTRYAG 1 cut(s) 426
SfoI GGCGCC 1 cut(s) 67
Sse9I AATT 4 cut(s) 73, 91, 152, 445
SsiI CCGC 3 cut(s) 11, 125, 191
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 1 cut(s) 383
SspMI CTAG 1 cut(s) 413
SstI GAGCTC 1 cut(s) 150
TaaI ACNGT 1 cut(s) 293
TaiI ACGT 2 cut(s) 41, 275
TaqI TCGA 2 cut(s) 144, 150
TaqII GACCGA 1 cut(s) 103
TasI AATT 4 cut(s) 73, 91, 152, 445
TatI WGTACW 1 cut(s) 21
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 3 cut(s) 57, 401, 472
TscAI CASTG 1 cut(s) 485
TspDTI ATGAA 3 cut(s) 145, 302, 372
TspRI CASTG 1 cut(s) 485
XapI RAATTY 2 cut(s) 152, 445
XceI RCATGY 1 cut(s) 22
XspI CTAG 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.